MD14G1220800.v1.1

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003632
Physical Location & Seq
Forward (+)
30261974 .. 30265215
3242 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1220800.v1.1.491

Sequence Viewer

Length: 651 bp
ATGGATGTGAGGAGAATAGTGGTAGTAGTTGAAGAAGTGGAAGCAGCAAGAACAGCTCTTCGATGGGCACTCCACAACCTGGTTAGGTTTGGAGACTGGGTAACTCTGGTTCATGTTTTTCCAACCTCAAGATCCAGAAACAAGAACAAATCCAGGCTTCTCCGCTTGAAAGGCTACCAGTTGACCCTCTCCTTCAAAGAAATCTGCAACACCTTCCCCAACACCAAGGTTGAAATGATTGTCACAGAAGGTGATCAAGATGGGATGAAGATTGCAGCTTTGGTCAGAGAGATTGGGGCTTCAGCTCTTGTTCTTGGACTCCATGATCACAGCTTTCTCTACAAGTTGGCAATGTCCCACGGCAGTACTAGTGTTGCAAACAACTTGAATTGCAGAGTTCTGGCCATCAAGCAACCACCACCTCCCTCATCACCATTAAGTAGTAAGAGGACAAGTAGTAGTAATACCAGTGGAGCTGCCACACCAGCCCTCCTCGATAGTTCAACCAACAACATGGACTTTTCACAGATTGACATTGCTGGATTACGTGTCCCTGATGTTCCTCAACCAAAAGTTCCATACAGAATCTGCCCAAACCCTTATGCAATCTTTTGGAAATCAAGGAAGTCAAGGAGAAAGAGAAATACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

217

Amino Acids

24.08

Weight (kDa)

10.25

Isoelectric Point (pI)

53.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 4 - 137 1.7e-14 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 79
AciI CCGC 1 cut(s) 163
AclWI GGATC 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 402
AcuI CTGAAG 1 cut(s) 285
AfaI GTAC 1 cut(s) 367
AfiI CCNNNNNNNGG 1 cut(s) 79
AflIII ACRYGT 1 cut(s) 547
AgsI TTSAA 6 cut(s) 32, 169, 196, 233, 388, 504
AhlI ACTAGT 1 cut(s) 368
AjnI CCWGG 2 cut(s) 78, 152
AluBI AGCT 5 cut(s) 56, 278, 305, 333, 476
AluI AGCT 5 cut(s) 56, 278, 305, 333, 476
Alw26I GTCTC 1 cut(s) 87
AlwI GGATC 1 cut(s) 126
AlwNI CAGNNNCTG 1 cut(s) 588
AoxI GGCC 1 cut(s) 402
ApeKI GCWGC 3 cut(s) 44, 275, 476
AsuHPI GGTGA 2 cut(s) 263, 423
BaeGI GKGCMC 1 cut(s) 70
BalI TGGCCA 1 cut(s) 404
BbvI GCAGC 3 cut(s) 56, 287, 463
BccI CCATC 3 cut(s) 57, 254, 413
BceAI ACGGC 1 cut(s) 376
BciT130I CCWGG 2 cut(s) 80, 154
BclI TGATCA 2 cut(s) 253, 325
BcoDI GTCTC 1 cut(s) 87
BcuI ACTAGT 1 cut(s) 368
BfaI CTAG 1 cut(s) 369
BisI GCNGC 3 cut(s) 45, 276, 477
BlsI GCNGC 3 cut(s) 46, 277, 478
BmcAI AGTACT 1 cut(s) 367
Bme1390I CCNGG 2 cut(s) 80, 154
BmrFI CCNGG 2 cut(s) 80, 154
BmrI ACTGGG 1 cut(s) 106
BmuI ACTGGG 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 112
BsaAI YACGTR 1 cut(s) 548
BsaJI CCNNGG 2 cut(s) 225, 358
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse1I ACTGG 3 cut(s) 101, 178, 468
Bse3DI GCAATG 2 cut(s) 357, 534
BseBI CCWGG 2 cut(s) 80, 154
BseDI CCNNGG 2 cut(s) 225, 358
BseGI GGATG 2 cut(s) 10, 270
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMI GCAATG 2 cut(s) 357, 534
BseNI ACTGG 3 cut(s) 101, 178, 468
BseRI GAGGAG 2 cut(s) 25, 482
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 3 cut(s) 56, 287, 463
BshFI GGCC 1 cut(s) 404
BslFI GGGAC 2 cut(s) 340, 536
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 1 cut(s) 87
BsmFI GGGAC 2 cut(s) 340, 536
BsnI GGCC 1 cut(s) 404
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 3 cut(s) 131, 253, 325
BspACI CCGC 1 cut(s) 163
BspANI GGCC 1 cut(s) 404
BspPI GGATC 1 cut(s) 126
BspQI GCTCTTC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 357, 534
BsrI ACTGG 3 cut(s) 101, 178, 468
BssECI CCNNGG 2 cut(s) 225, 358
BssMI GATC 3 cut(s) 131, 253, 325
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 2 cut(s) 80, 154
Bst6I CTCTTC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 548
BstDSI CCRYGG 1 cut(s) 358
BstF5I GGATG 2 cut(s) 10, 270
BstKTI GATC 3 cut(s) 134, 256, 328
BstMAI GTCTC 1 cut(s) 87
BstMBI GATC 3 cut(s) 131, 253, 325
BstMWI GCNNNNNNNGC 3 cut(s) 53, 171, 485
BstNI CCWGG 2 cut(s) 80, 154
BstSCI CCNGG 2 cut(s) 78, 152
BstSLI GKGCMC 1 cut(s) 70
BstV1I GCAGC 3 cut(s) 56, 287, 463
BstX2I RGATCY 1 cut(s) 131
BstXI CCANNNNNNTGG 1 cut(s) 514
BstYI RGATCY 1 cut(s) 131
BsuRI GGCC 1 cut(s) 404
BtgI CCRYGG 1 cut(s) 358
BtsCI GGATG 2 cut(s) 10, 270
BtsIMutI CAGTG 1 cut(s) 475
CaiI CAGNNNCTG 1 cut(s) 588
CsiI ACCWGGT 1 cut(s) 78
Csp6I GTAC 1 cut(s) 366
CviAII CATG 3 cut(s) 113, 323, 514
CviQI GTAC 1 cut(s) 366
DpnI GATC 3 cut(s) 133, 255, 327
DpnII GATC 3 cut(s) 131, 253, 325
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 225
Eco57I CTGAAG 1 cut(s) 285
EcoRII CCWGG 2 cut(s) 78, 152
EcoT14I CCWWGG 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 3 cut(s) 116, 326, 517
FaiI YATR 6 cut(s) 114, 324, 515, 580, 603, 649
FaqI GGGAC 2 cut(s) 340, 536
FatI CATG 3 cut(s) 112, 322, 513
FbaI TGATCA 2 cut(s) 253, 325
Fnu4HI GCNGC 3 cut(s) 45, 276, 477
FokI GGATG 2 cut(s) 17, 277
Fsp4HI GCNGC 3 cut(s) 45, 276, 477
FspBI CTAG 1 cut(s) 369
GluI GCNGC 3 cut(s) 45, 276, 477
HaeIII GGCC 1 cut(s) 404
Hin1II CATG 3 cut(s) 116, 326, 517
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HinfI GANTC 2 cut(s) 318, 585
HphI GGTGA 2 cut(s) 263, 423
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 1 cut(s) 287
Hpy188III TCNNGA 3 cut(s) 129, 135, 257
Hpy8I GTNNAC 1 cut(s) 183
HpyAV CCTTC 3 cut(s) 202, 223, 242
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 5 cut(s) 207, 275, 377, 393, 605
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 171, 485
HpySE526I ACGT 1 cut(s) 547
Hsp92II CATG 3 cut(s) 116, 326, 517
Ksp22I TGATCA 2 cut(s) 253, 325
Kzo9I GATC 3 cut(s) 131, 253, 325
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 1 cut(s) 473
Lsp1109I GCAGC 3 cut(s) 56, 287, 463
MabI ACCWGGT 1 cut(s) 78
MaeI CTAG 1 cut(s) 369
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 2 cut(s) 100, 241
MalI GATC 3 cut(s) 133, 255, 327
MboI GATC 3 cut(s) 131, 253, 325
MboII GAAGA 3 cut(s) 44, 50, 280
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 1 cut(s) 70
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 1 cut(s) 388
MluNI TGGCCA 1 cut(s) 404
MlyI GAGTC 1 cut(s) 312
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 9 cut(s) 3, 136, 197, 432, 436, 441, 500, 503, 573
Mox20I TGGCCA 1 cut(s) 404
MscI TGGCCA 1 cut(s) 404
MseI TTAA 1 cut(s) 437
Msp20I TGGCCA 1 cut(s) 404
MspR9I CCNGG 2 cut(s) 80, 154
MvaI CCWGG 2 cut(s) 80, 154
MwoI GCNNNNNNNGC 3 cut(s) 53, 171, 485
NdeII GATC 3 cut(s) 131, 253, 325
NlaIII CATG 3 cut(s) 116, 326, 517
NmuCI GTSAC 1 cut(s) 241
PciSI GCTCTTC 1 cut(s) 63
PfeI GAWTC 1 cut(s) 585
PflMI CCANNNNNTGG 1 cut(s) 79
PkrI GCNGC 3 cut(s) 46, 277, 478
PleI GAGTC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 312
Ppu21I YACGTR 1 cut(s) 548
Psp6I CCWGG 2 cut(s) 78, 152
PspGI CCWGG 2 cut(s) 78, 152
PsrI GAACNNNNNNTAC 2 cut(s) 93, 125
PstNI CAGNNNCTG 1 cut(s) 588
PsuI RGATCY 1 cut(s) 131
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 3 cut(s) 45, 276, 477
Sau3AI GATC 3 cut(s) 131, 253, 325
ScaI AGTACT 1 cut(s) 367
SchI GAGTC 1 cut(s) 312
ScrFI CCNGG 2 cut(s) 80, 154
SduI GDGCHC 1 cut(s) 70
SexAI ACCWGGT 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
SpeI ACTAGT 1 cut(s) 368
Sse9I AATT 1 cut(s) 388
SsiI CCGC 1 cut(s) 163
SspMI CTAG 1 cut(s) 369
StyD4I CCNGG 2 cut(s) 78, 152
StyI CCWWGG 1 cut(s) 225
TaiI ACGT 1 cut(s) 550
TaqI TCGA 2 cut(s) 61, 495
TasI AATT 1 cut(s) 388
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 1 cut(s) 585
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 1 cut(s) 475
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 3 cut(s) 44, 275, 476
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 2 cut(s) 101, 281
TspRI CASTG 1 cut(s) 475
Van91I CCANNNNNTGG 1 cut(s) 79
XspI CTAG 1 cut(s) 369
ZrmI AGTACT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.