Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004021

Physical Location & Seq

Genomic Coordinates Reverse (-)

31739867 .. 31741372

Gene Structure 1506 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1313900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGGATCCACCTGTGGCACAAACCATCACCGACCCTTCAAGCGTCACTGATTTTGTTATAAGCAAAAGTCATGGAGTAAAGGGTCTTTCAGAAATGGGACTAAAAGCCCTCCCCAAGCAATATATCCAACCCCTTGAAGAAAGAATTACTACTATGAATGTTGTGGCCACAACAAACCCTGGAGAGTACATTCCCATCATCGACATGTCTAACTCCGATGACCCAAAAGTTGCAGAAGCAATTTGCAATGCAGCGGAGAAGTGGGGGTTCTTTCAACTAGTGAATCATGGGGTGCCAATTGAAGAGCTTGAGAATGTGATGGAAGCAACTCATCGCTTCTTCGAGTTGCCAGCCGAGAAGAAGACTAAGTATTCGGTTGAGCATTCGGCCTCAAAAAATGTGCGGTTTGGAACGAGCTTTAGTCCTCATGCAGAGAAGGCTTTGGAGTGGAAAGATTGGCTTAGTCTATTCTATGTGTCCGAGGATGAGGCCTCTGCATTCTGGCCTCCTGCATGCAAGGCTGAAGTGCTGGAATACATGAAGAAGACCGACATTCTTACAAAACAAATCCTAGAAATGCTAATGAAAAGACTAAACGTGGAAGAACTTGACCAAGAAAAGGAACATCTTCTAATGGGATCCAAGCGGATTAACCTTAACTACTACCCGGTGTGTCCAAACCCTGAGCTCACGGTTGGAGTCGGCCGTCACTCCGATGTCTCAACACTCACAATTCTCCTCCAAGACCACATCGGTGGACTTTACGTCCGCGGAACCGATAAGGAGAGCTGGGTTCATGTTCCGCCAGTGAAGGGGTCGTTGGTGATCAACATCGGCGATGCACTGCAAATAATGAGCAACGGACGATACAAGAGCATCGAGCATCGAGTCGCAGCCAATGGATGTAAGAACAGGATTTCGGTTCCCATTTTTGTCAACCCAAGGCCGTCGGATTTGATCGCTCCGCTGCCGGAAGTTCTTGCAAGTGGGGAGAAAGCAGTGTATAAACAAGTTCTCTACTCTGACTATGTCAGACATTTCTTCCGCAAGGCTCATGATGGGAAGGGTACAATTGACTTTGCCAAAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0003824 GO:0006725 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009636 GO:0009698 GO:0009699 GO:0009804 GO:0009805 GO:0009987 GO:0010035 GO:0010421 GO:0012501 GO:0016491 GO:0018130 GO:0019438 GO:0019748 GO:0033554 GO:0034599 GO:0034614 GO:0035690 GO:0036473 GO:0036474 GO:0042221 GO:0042493 GO:0042542 GO:0044237 GO:0044249 GO:0044550 GO:0046483 GO:0046677 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0070301 GO:0070887 GO:0071236 GO:0071704 GO:0097237 GO:0097468 GO:1901360 GO:1901362 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00940 ko01110 ko04110 ko04111 ko04114 ko04120 ko04141 ko04310 ko04341 ko04350 ko04710 ko05168 ko05200 map00940 map01110 map04110 map04111 map04114 map04120 map04141 map04310 map04341 map04350 map04710 map05168 map05200

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

364

Amino Acids

40.7

Weight (kDa)

6.08

Isoelectric Point (pI)

39.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	64 - 168	1.5e-25	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	218 - 314	4.6e-31	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004021)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55290 AT3G13610
fragaria_vesca	FvH4_3g45310
malus_domestica	MD03G1006400.v1.1 MD15G1313900.v1.1
prunus_persica	Prupe.6G006000_v2.0.a1
pyrus_communis	pycom03g00660
rosa_chinensis	RchiOBHm_Chr5g0082981 RchiOBHm_Chr5g0082991 RchiOBHm_Chr5g0083061 RchiOBHm_Chr5g0083081
rosa_laevigata	RLG00000028679 RLG00000037007 RLG00000037009
rosa_multiflora	Rmu_sc0003727.1_g000002 Rmu_sc0003727.1_g000007 Rmu_sc0003727.1_g000008
rosa_roxburghii	Rroxscaffold_1G00001420 Rroxscaffold_1G00001430 Rroxscaffold_1G00001440
rosa_rugosa	Rorug05G0481900
rosa_samantha	Rh5AG531500 Rh5AG531600 Rh5BG558600 Rh5BG558700 Rh5CG581500 Rh5CG581600 Rh5DG567300 Rh5DG567400 Rh5DG567600
rosa_wichuraiana	Rw5G049670 Rw5G049700 Rw5G049710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	59
AccB1I	GGYRCC	1 cut(s)	292
AccII	CGCG	1 cut(s)	771
AciI	CCGC	8 cut(s)	254, 403, 646, 769, 771, 803, 965, 1045
AclWI	GGATC	3 cut(s)	12, 633, 646
AcoI	YGGCCR	2 cut(s)	165, 703
AcuI	CTGAAG	1 cut(s)	543
AfaI	GTAC	2 cut(s)	188, 1069
AfiI	CCNNNNNNNGG	2 cut(s)	619, 812
AflIII	ACRYGT	1 cut(s)	204
AgsI	TTSAA	4 cut(s)	39, 137, 275, 302
AhdI	GACNNNNNGTC	1 cut(s)	764
AhlI	ACTAGT	1 cut(s)	277
AjnI	CCWGG	1 cut(s)	178
AjuI	GAANNNNNNNTTGG	2 cut(s)	803, 835
AleI	CACNNNNGTG	1 cut(s)	753
AluBI	AGCT	4 cut(s)	307, 417, 688, 789
AluI	AGCT	4 cut(s)	307, 417, 688, 789
Alw21I	GWGCWC	1 cut(s)	690
Alw26I	GTCTC	1 cut(s)	724
AlwI	GGATC	3 cut(s)	12, 633, 646
AoxI	GGCC	6 cut(s)	165, 387, 489, 503, 703, 944
ApeKI	GCWGC	3 cut(s)	251, 893, 967
ArsI	GACNNNNNNTTYG	2 cut(s)	355, 387
Asp700I	GAANNNNTTC	1 cut(s)	627
AsuC2I	CCSGG	1 cut(s)	668
AsuHPI	GGTGA	2 cut(s)	19, 835
BalI	TGGCCA	1 cut(s)	167
BamHI	GGATCC	2 cut(s)	4, 638
BanI	GGYRCC	1 cut(s)	292
BanII	GRGCYC	1 cut(s)	690
BbsI	GAAGAC	2 cut(s)	368, 551
Bbv12I	GWGCWC	1 cut(s)	690
BbvI	GCAGC	3 cut(s)	263, 905, 954
BccI	CCATC	4 cut(s)	32, 203, 313, 1052
BceAI	ACGGC	2 cut(s)	690, 931
BciT130I	CCWGG	1 cut(s)	180
BclI	TGATCA	1 cut(s)	825
BcnI	CCSGG	1 cut(s)	668
BcoDI	GTCTC	1 cut(s)	724
BcuI	ACTAGT	1 cut(s)	277
BfaI	CTAG	2 cut(s)	278, 572
BisI	GCNGC	3 cut(s)	252, 894, 968
BlsI	GCNGC	3 cut(s)	253, 895, 969
Bme1390I	CCNGG	2 cut(s)	180, 668
BmeRI	GACNNNNNGTC	1 cut(s)	764
BmiI	GGNNCC	5 cut(s)	6, 294, 640, 775, 924
BmrFI	CCNGG	2 cut(s)	180, 668
BmsI	GCATC	3 cut(s)	829, 885, 892
BpiI	GAAGAC	2 cut(s)	368, 551
BpmI	CTGGAG	1 cut(s)	201
Bpu10I	CCTNAGC	1 cut(s)	684
BpuEI	CTTGAG	1 cut(s)	329
BpuMI	CCSGG	1 cut(s)	668
BsaBI	GATNNNNATC	1 cut(s)	830
BsaJI	CCNNGG	4 cut(s)	178, 480, 769, 941
Bsc4I	CCNNNNNNNGG	2 cut(s)	619, 812
Bse1I	ACTGG	1 cut(s)	806
Bse3DI	GCAATG	1 cut(s)	253
Bse8I	GATNNNNATC	1 cut(s)	830
BseBI	CCWGG	1 cut(s)	180
BseDI	CCNNGG	4 cut(s)	178, 480, 769, 941
BseGI	GGATG	2 cut(s)	490, 908
BseJI	GATNNNNATC	1 cut(s)	830
BseLI	CCNNNNNNNGG	2 cut(s)	619, 812
BseMI	GCAATG	1 cut(s)	253
BseMII	CTCAG	1 cut(s)	675
BseNI	ACTGG	1 cut(s)	806
BseRI	GAGGAG	1 cut(s)	728
BseX3I	CGGCCG	1 cut(s)	703
BseXI	GCAGC	3 cut(s)	263, 905, 954
BseYI	CCCAGC	1 cut(s)	789
Bsh1236I	CGCG	1 cut(s)	771
Bsh1285I	CGRYCG	1 cut(s)	706
BshFI	GGCC	6 cut(s)	167, 389, 491, 505, 705, 946
BshNI	GGYRCC	1 cut(s)	292
BsiEI	CGRYCG	1 cut(s)	706
BsiHKAI	GWGCWC	1 cut(s)	690
BsiSI	CCGG	2 cut(s)	668, 971
BslFI	GGGAC	1 cut(s)	111
BslI	CCNNNNNNNGG	2 cut(s)	619, 812
BsmAI	GTCTC	1 cut(s)	724
BsmFI	GGGAC	1 cut(s)	111
BsmI	GAATGC	2 cut(s)	382, 497
BsnI	GGCC	6 cut(s)	167, 389, 491, 505, 705, 946
Bsp1286I	GDGCHC	1 cut(s)	690
Bsp143I	GATC	4 cut(s)	4, 638, 825, 957
BspACI	CCGC	8 cut(s)	254, 403, 646, 769, 771, 803, 965, 1045
BspANI	GGCC	6 cut(s)	167, 389, 491, 505, 705, 946
BspCNI	CTCAG	1 cut(s)	676
BspFNI	CGCG	1 cut(s)	771
BspHI	TCATGA	1 cut(s)	1054
BspLI	GGNNCC	5 cut(s)	6, 294, 640, 775, 924
BspPI	GGATC	3 cut(s)	12, 633, 646
BspQI	GCTCTTC	1 cut(s)	297
BspT107I	GGYRCC	1 cut(s)	292
BsrDI	GCAATG	1 cut(s)	253
BsrI	ACTGG	1 cut(s)	806
BssECI	CCNNGG	4 cut(s)	178, 480, 769, 941
BssMI	GATC	4 cut(s)	4, 638, 825, 957
BssT1I	CCWWGG	1 cut(s)	941
Bst2UI	CCWGG	1 cut(s)	180
Bst4CI	ACNGT	1 cut(s)	694
Bst6I	CTCTTC	1 cut(s)	297
BstC8I	GCNNGC	2 cut(s)	351, 514
BstDEI	CTNAG	3 cut(s)	366, 461, 684
BstDSI	CCRYGG	1 cut(s)	769
BstF5I	GGATG	2 cut(s)	490, 908
BstFNI	CGCG	1 cut(s)	771
BstKTI	GATC	4 cut(s)	7, 641, 828, 960
BstMAI	GTCTC	1 cut(s)	724
BstMBI	GATC	4 cut(s)	4, 638, 825, 957
BstMCI	CGRYCG	1 cut(s)	706
BstMWI	GCNNNNNNNGC	2 cut(s)	437, 518
BstNI	CCWGG	1 cut(s)	180
BstNSI	RCATGY	2 cut(s)	208, 516
BstSCI	CCNGG	2 cut(s)	178, 666
BstUI	CGCG	1 cut(s)	771
BstV1I	GCAGC	3 cut(s)	263, 905, 954
BstV2I	GAAGAC	2 cut(s)	368, 551
BstX2I	RGATCY	2 cut(s)	4, 638
BstXI	CCANNNNNNTGG	1 cut(s)	755
BstYI	RGATCY	2 cut(s)	4, 638
BstZI	CGGCCG	1 cut(s)	703
BsuRI	GGCC	6 cut(s)	167, 389, 491, 505, 705, 946
BtgI	CCRYGG	1 cut(s)	769
BtgZI	GCGATG	2 cut(s)	317, 852
BtsCI	GGATG	2 cut(s)	490, 908
BtsI	GCAGTG	2 cut(s)	842, 1005
BtsIMutI	CAGTG	4 cut(s)	45, 813, 842, 1005
Cac8I	GCNNGC	2 cut(s)	351, 514
CciI	TCATGA	1 cut(s)	1054
Cfr42I	CCGCGG	1 cut(s)	772
CseI	GACGC	1 cut(s)	31
Csp6I	GTAC	2 cut(s)	187, 1068
CviAII	CATG	9 cut(s)	71, 205, 287, 428, 513, 538, 797, 1055, 1089
CviQI	GTAC	2 cut(s)	187, 1068
DdeI	CTNAG	3 cut(s)	366, 461, 684
DpnI	GATC	4 cut(s)	6, 640, 827, 959
DpnII	GATC	4 cut(s)	4, 638, 825, 957
DriI	GACNNNNNGTC	1 cut(s)	764
EaeI	YGGCCR	2 cut(s)	165, 703
EagI	CGGCCG	1 cut(s)	703
Eam1104I	CTCTTC	1 cut(s)	297
Eam1105I	GACNNNNNGTC	1 cut(s)	764
EarI	CTCTTC	1 cut(s)	297
EciI	GGCGGA	1 cut(s)	792
Ecl136II	GAGCTC	1 cut(s)	688
EclXI	CGGCCG	1 cut(s)	703
Eco130I	CCWWGG	1 cut(s)	941
Eco147I	AGGCCT	1 cut(s)	491
Eco24I	GRGCYC	1 cut(s)	690
Eco52I	CGGCCG	1 cut(s)	703
Eco53kI	GAGCTC	1 cut(s)	688
Eco57I	CTGAAG	1 cut(s)	543
EcoICRI	GAGCTC	1 cut(s)	688
EcoRII	CCWGG	1 cut(s)	178
EcoT14I	CCWWGG	1 cut(s)	941
EcoT38I	GRGCYC	1 cut(s)	690
ErhI	CCWWGG	1 cut(s)	941
FaeI	CATG	9 cut(s)	74, 208, 290, 431, 516, 541, 800, 1058, 1092
FalI	AAGNNNNNCTT	2 cut(s)	444, 476
FaqI	GGGAC	1 cut(s)	111
FatI	CATG	9 cut(s)	70, 204, 286, 427, 512, 537, 796, 1054, 1088
FbaI	TGATCA	1 cut(s)	825
Fnu4HI	GCNGC	3 cut(s)	252, 894, 968
FokI	GGATG	2 cut(s)	497, 915
FriOI	GRGCYC	1 cut(s)	690
Fsp4HI	GCNGC	3 cut(s)	252, 894, 968
FspBI	CTAG	2 cut(s)	278, 572
GluI	GCNGC	3 cut(s)	252, 894, 968
GsaI	CCCAGC	1 cut(s)	793
GsuI	CTGGAG	1 cut(s)	201
HaeIII	GGCC	6 cut(s)	167, 389, 491, 505, 705, 946
HapII	CCGG	2 cut(s)	668, 971
HgaI	GACGC	1 cut(s)	31
Hin1II	CATG	9 cut(s)	74, 208, 290, 431, 516, 541, 800, 1058, 1092
HincII	GTYRAC	1 cut(s)	937
HindII	GTYRAC	1 cut(s)	937
HinfI	GANTC	3 cut(s)	283, 699, 888
HpaII	CCGG	2 cut(s)	668, 971
HphI	GGTGA	2 cut(s)	19, 835
Hpy166II	GTNNAC	2 cut(s)	758, 937
Hpy188I	TCNGA	7 cut(s)	91, 217, 481, 715, 952, 1024, 1034
Hpy188III	TCNNGA	1 cut(s)	1055
Hpy8I	GTNNAC	2 cut(s)	758, 937
Hpy99I	CGWCG	1 cut(s)	952
HpyAV	CCTTC	4 cut(s)	45, 430, 805, 1057
HpyCH4III	ACNGT	1 cut(s)	694
HpyCH4IV	ACGT	2 cut(s)	597, 765
HpyF10VI	GCNNNNNNNGC	2 cut(s)	437, 518
HpyF3I	CTNAG	3 cut(s)	366, 461, 684
HpySE526I	ACGT	2 cut(s)	597, 765
Hsp92II	CATG	9 cut(s)	74, 208, 290, 431, 516, 541, 800, 1058, 1092
Ksp22I	TGATCA	1 cut(s)	825
KspI	CCGCGG	1 cut(s)	772
Kzo9I	GATC	4 cut(s)	4, 638, 825, 957
LguI	GCTCTTC	1 cut(s)	297
LmnI	GCTCC	1 cut(s)	967
Lsp1109I	GCAGC	3 cut(s)	263, 905, 954
LweI	GCATC	3 cut(s)	829, 885, 892
MaeI	CTAG	2 cut(s)	278, 572
MaeII	ACGT	2 cut(s)	597, 765
MaeIII	GTNAC	2 cut(s)	43, 707
MalI	GATC	4 cut(s)	6, 640, 827, 959
MboI	GATC	4 cut(s)	4, 638, 825, 957
MfeI	CAATTG	2 cut(s)	297, 1071
MflI	RGATCY	2 cut(s)	4, 638
MhlI	GDGCHC	1 cut(s)	690
MlsI	TGGCCA	1 cut(s)	167
MluCI	AATT	5 cut(s)	144, 240, 297, 732, 1071
MluNI	TGGCCA	1 cut(s)	167
MlyI	GAGTC	2 cut(s)	708, 897
MmeI	TCCRAC	3 cut(s)	151, 676, 930
MnlI	CCTC	8 cut(s)	119, 400, 435, 475, 481, 502, 516, 749
Mox20I	TGGCCA	1 cut(s)	167
MroXI	GAANNNNTTC	1 cut(s)	627
MscI	TGGCCA	1 cut(s)	167
MseI	TTAA	2 cut(s)	651, 657
MslI	CAYNNNNRTG	3 cut(s)	203, 714, 753
Msp20I	TGGCCA	1 cut(s)	167
MspA1I	CMGCKG	3 cut(s)	254, 771, 967
MspI	CCGG	2 cut(s)	668, 971
MspR9I	CCNGG	2 cut(s)	180, 668
MunI	CAATTG	2 cut(s)	297, 1071
Mva1269I	GAATGC	2 cut(s)	382, 497
MvaI	CCWGG	1 cut(s)	180
MvnI	CGCG	1 cut(s)	771
MwoI	GCNNNNNNNGC	2 cut(s)	437, 518
NciI	CCSGG	1 cut(s)	668
NdeII	GATC	4 cut(s)	4, 638, 825, 957
NlaIII	CATG	9 cut(s)	74, 208, 290, 431, 516, 541, 800, 1058, 1092
NlaIV	GGNNCC	5 cut(s)	6, 294, 640, 775, 924
NmeAIII	GCCGAG	1 cut(s)	379
NmuCI	GTSAC	2 cut(s)	43, 707
NspI	RCATGY	2 cut(s)	208, 516
OliI	CACNNNNGTG	1 cut(s)	753
PaeI	GCATGC	1 cut(s)	516
PagI	TCATGA	1 cut(s)	1054
PceI	AGGCCT	1 cut(s)	491
PciI	ACATGT	1 cut(s)	204
PciSI	GCTCTTC	1 cut(s)	297
PctI	GAATGC	2 cut(s)	382, 497
PdmI	GAANNNNTTC	1 cut(s)	627
PfeI	GAWTC	1 cut(s)	283
PflFI	GACNNNGTC	1 cut(s)	1028
PkrI	GCNGC	3 cut(s)	253, 895, 969
PleI	GAGTC	2 cut(s)	707, 896
PpsI	GAGTC	2 cut(s)	707, 896
PscI	ACATGT	1 cut(s)	204
PsiI	TTATAA	1 cut(s)	59
Psp124BI	GAGCTC	1 cut(s)	690
Psp6I	CCWGG	1 cut(s)	178
PspFI	CCCAGC	1 cut(s)	789
PspGI	CCWGG	1 cut(s)	178
PspN4I	GGNNCC	5 cut(s)	6, 294, 640, 775, 924
PsuI	RGATCY	2 cut(s)	4, 638
PsyI	GACNNNGTC	1 cut(s)	1028
RsaI	GTAC	2 cut(s)	188, 1069
RsaNI	GTAC	2 cut(s)	187, 1068
RseI	CAYNNNNRTG	3 cut(s)	203, 714, 753
SacI	GAGCTC	1 cut(s)	690
SacII	CCGCGG	1 cut(s)	772
SapI	GCTCTTC	1 cut(s)	297
SaqAI	TTAA	2 cut(s)	651, 657
SatI	GCNGC	3 cut(s)	252, 894, 968
Sau3AI	GATC	4 cut(s)	4, 638, 825, 957
SchI	GAGTC	2 cut(s)	708, 897
ScrFI	CCNGG	2 cut(s)	180, 668
SduI	GDGCHC	1 cut(s)	690
SetI	ASST	8 cut(s)	13, 309, 419, 600, 657, 690, 768, 791
SfaNI	GCATC	3 cut(s)	829, 885, 892
Sfr303I	CCGCGG	1 cut(s)	772
SgrBI	CCGCGG	1 cut(s)	772
SmiMI	CAYNNNNRTG	3 cut(s)	203, 714, 753
SmlI	CTYRAG	1 cut(s)	308
SmoI	CTYRAG	1 cut(s)	308
SpeI	ACTAGT	1 cut(s)	277
SphI	GCATGC	1 cut(s)	516
Sse9I	AATT	5 cut(s)	144, 240, 297, 732, 1071
SseBI	AGGCCT	1 cut(s)	491
SsiI	CCGC	8 cut(s)	254, 403, 646, 769, 771, 803, 965, 1045
SspMI	CTAG	2 cut(s)	278, 572
SstI	GAGCTC	1 cut(s)	690
StuI	AGGCCT	1 cut(s)	491
StyD4I	CCNGG	2 cut(s)	178, 666
StyI	CCWWGG	1 cut(s)	941
TaaI	ACNGT	1 cut(s)	694
TaiI	ACGT	2 cut(s)	600, 768
TaqI	TCGA	4 cut(s)	201, 342, 879, 886
TaqII	GACCGA	1 cut(s)	563
TasI	AATT	5 cut(s)	144, 240, 297, 732, 1071
TatI	WGTACW	1 cut(s)	186
TfiI	GAWTC	1 cut(s)	283
Tru1I	TTAA	2 cut(s)	651, 657
Tru9I	TTAA	2 cut(s)	651, 657
TscAI	CASTG	4 cut(s)	52, 813, 849, 1005
TseFI	GTSAC	2 cut(s)	43, 707
TseI	GCWGC	3 cut(s)	251, 893, 967
Tsp45I	GTSAC	2 cut(s)	43, 707
TspDTI	ATGAA	4 cut(s)	170, 554, 599, 785
TspGWI	ACGGA	1 cut(s)	876
TspRI	CASTG	4 cut(s)	52, 813, 849, 1005
Tth111I	GACNNNGTC	1 cut(s)	1028
XceI	RCATGY	2 cut(s)	208, 516
XmnI	GAANNNNTTC	1 cut(s)	627
XspI	CTAG	2 cut(s)	278, 572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.