MD15G1379800.v1.1

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002383
Physical Location & Seq
Reverse (-)
46720042 .. 46724017
3976 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1379800.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGCCCAGTATACTCCAACCTTAAACCCTCCCAAAGCCCTAATCCTCAAGCGCTCAAATCCCGATCCCTCTTTCTCTTCCTTCTCAACATTCACAACCCCAAAACCCAAATCCATTAACTTCCCAAAATTCTCCATGTCAGTCACGGCGGCCCAAAACCAGCAGCACTACCAGGAACCGCCGTCCTTGGAAGCTCTAATCAACGGTGCTCGCAAAGAAGAGGTGCTTGGCGTTATCAAGAGTTCGTCGTTTCACTGCCTCTCCGAAACCAACCTCCACCTCACAGTTCCTGGCCTCAAATCCAAAATTAGAGGCAAGGTTAGAGATGTTTATGACAATGGGGATTATCTAGTTATGGTTACGACGGATAGGCAGAGTGCATTTGACAGAATTCTTGCTTCCATTCCCTTCAAAGGACAGGTCCTTAATGAGACAAGTTTATGGTGGTTTGACAAAACTCAACACATAACTTCGAACGCAGTTGTTTCGGCTCCAGATGAAAATGTAACAATTGCAAAGAAATGTTCTGTTTTTCCCGTTGAGTTTGTGGTCAGAGGTTTTGTGACGGGAAGTACTGATACGTCATTATGGACGGTCTACAAAAATGGTGCTCGAAATTACTGTGGCAATGTACTCCCAGATGGCTTGGTAAAAAGCCAAAAGCTTCCTGCAAATATACTCACACCGACAACTAAGGCTGCAGATCATGATGTTCCCGTTACCCCAGATGAGATAATTGAACGCGGACTAATGACTCAAGCTGAGTATGATGAAGTAAGCAGGAAAGCACTAAGCCTGTTTGAGTACGGACAGCATGTGGCATTGGAACATGGCTTGATATTGGTAGACACAAAATATGAATTCGGAAAGGGCCAGGATGGTTCCATTCTTTTGATTGATGAGGTGCATACACCTGATTCAAGTAGATATTGGATTGCACATACTTATGAGGAGCGATTTCAGAATGGTCTTGAACCTGAAAATGTTGATAAAGAGTTCTTGAGGTTGTGGTTCAAAGATCACTGCAATCCTTACGAAGATGAGGTCCTCCCTGATGCTCCTGAGGAACTTGTTTGTGAACTAGCTTGGCGATACATTTTCCTGTACGAGACAATAACAAAATCAAAATTCAAGTTGCCCTCCACAGAGGAACCAATACATGATCGGATATCACGAAATGTTGCGCGGGCGTTGTCATCTCTGGCTCGATAA

Protein Analysis

404

Amino Acids

45.51

Weight (kDa)

5.82

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAICAR_synt PF01259 103 - 348 2e-69 SAICAR synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 11, 597, 846
AccII CGCG 2 cut(s) 744, 1186
AciI CCGC 4 cut(s) 149, 179, 744, 1186
AclWI GGATC 1 cut(s) 59
AcsI RAATTY 4 cut(s) 128, 390, 860, 1127
AfaI GTAC 4 cut(s) 574, 633, 806, 1106
AfeI AGCGCT 1 cut(s) 53
AfiI CCNNNNNNNGG 1 cut(s) 413
AgsI TTSAA 6 cut(s) 412, 740, 921, 974, 1015, 1132
AjnI CCWGG 3 cut(s) 171, 289, 873
AjuI GAANNNNNNNTTGG 2 cut(s) 210, 242
AluBI AGCT 4 cut(s) 194, 664, 761, 1085
AluI AGCT 4 cut(s) 194, 664, 761, 1085
Alw21I GWGCWC 2 cut(s) 211, 613
Alw26I GTCTC 2 cut(s) 425, 1103
AlwI GGATC 1 cut(s) 59
AlwNI CAGNNNCTG 1 cut(s) 290
Aor51HI AGCGCT 1 cut(s) 53
AoxI GGCC 4 cut(s) 3, 150, 292, 871
ApeKI GCWGC 2 cut(s) 163, 698
ApoI RAATTY 4 cut(s) 128, 390, 860, 1127
ArsI GACNNNNNNTTYG 4 cut(s) 405, 437, 1029, 1061
AspLEI GCGC 2 cut(s) 54, 1186
AspS9I GGNCC 5 cut(s) 4, 151, 421, 871, 1045
AsuII TTCGAA 1 cut(s) 473
AvaII GGWCC 2 cut(s) 421, 1045
AxyI CCTNAGG 1 cut(s) 1062
BarI GAAGNNNNNNTAC 2 cut(s) 562, 594
Bbv12I GWGCWC 2 cut(s) 211, 613
BbvI GCAGC 2 cut(s) 175, 685
BccI CCATC 2 cut(s) 635, 872
BceAI ACGGC 2 cut(s) 162, 166
BcgI CGANNNNNNTGC 2 cut(s) 468, 502
BciT130I CCWGG 3 cut(s) 173, 291, 875
BcoDI GTCTC 2 cut(s) 425, 1103
BfaI CTAG 2 cut(s) 350, 1082
BfmI CTRYAG 1 cut(s) 699
BfoI RGCGCY 1 cut(s) 55
BisI GCNGC 3 cut(s) 150, 164, 699
BlsI GCNGC 3 cut(s) 151, 165, 700
BmcAI AGTACT 1 cut(s) 574
Bme1390I CCNGG 3 cut(s) 173, 291, 875
Bme18I GGWCC 2 cut(s) 421, 1045
BmgT120I GGNCC 5 cut(s) 4, 151, 421, 871, 1045
BmiI GGNNCC 4 cut(s) 177, 492, 883, 1152
BmrFI CCNGG 3 cut(s) 173, 291, 875
BmsI GCATC 1 cut(s) 1045
BpmI CTGGAG 1 cut(s) 477
Bpu14I TTCGAA 1 cut(s) 473
BpuEI CTTGAG 3 cut(s) 32, 741, 1021
BsaJI CCNNGG 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse1I ACTGG 1 cut(s) 7
Bse21I CCTNAGG 1 cut(s) 1062
Bse3DI GCAATG 1 cut(s) 634
BseBI CCWGG 3 cut(s) 173, 291, 875
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 883
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMI GCAATG 1 cut(s) 634
BseMII CTCAG 2 cut(s) 753, 1053
BseNI ACTGG 1 cut(s) 7
BseRI GAGGAG 1 cut(s) 965
BseXI GCAGC 2 cut(s) 175, 685
Bsh1236I CGCG 2 cut(s) 744, 1186
BshFI GGCC 4 cut(s) 5, 152, 294, 873
BsiHKAI GWGCWC 2 cut(s) 211, 613
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 2 cut(s) 425, 1103
BsnI GGCC 4 cut(s) 5, 152, 294, 873
Bsp119I TTCGAA 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 211, 613
Bsp143I GATC 4 cut(s) 64, 703, 1018, 1162
BspACI CCGC 4 cut(s) 149, 179, 744, 1186
BspANI GGCC 4 cut(s) 5, 152, 294, 873
BspCNI CTCAG 2 cut(s) 754, 1054
BspFNI CGCG 2 cut(s) 744, 1186
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 4 cut(s) 177, 492, 883, 1152
BspMAI CTGCAG 1 cut(s) 703
BspPI GGATC 1 cut(s) 59
BspT104I TTCGAA 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 634
BsrI ACTGG 1 cut(s) 7
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 4 cut(s) 64, 703, 1018, 1162
BssNAI GTATAC 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 186
Bst1107I GTATAC 1 cut(s) 12
Bst2UI CCWGG 3 cut(s) 173, 291, 875
Bst4CI ACNGT 4 cut(s) 206, 286, 595, 623
Bst6I CTCTTC 2 cut(s) 82, 213
BstBI TTCGAA 1 cut(s) 473
BstC8I GCNNGC 2 cut(s) 211, 1188
BstDEI CTNAG 4 cut(s) 693, 762, 791, 1062
BstF5I GGATG 1 cut(s) 883
BstFNI CGCG 2 cut(s) 744, 1186
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 2 cut(s) 54, 1186
BstKTI GATC 4 cut(s) 67, 706, 1021, 1165
BstMAI GTCTC 2 cut(s) 425, 1103
BstMBI GATC 4 cut(s) 64, 703, 1018, 1162
BstNI CCWGG 3 cut(s) 173, 291, 875
BstNSI RCATGY 1 cut(s) 818
BstSCI CCNGG 3 cut(s) 171, 289, 873
BstSFI CTRYAG 1 cut(s) 699
BstUI CGCG 2 cut(s) 744, 1186
BstV1I GCAGC 2 cut(s) 175, 685
BstZ17I GTATAC 1 cut(s) 12
Bsu36I CCTNAGG 1 cut(s) 1062
BsuRI GGCC 4 cut(s) 5, 152, 294, 873
BtsCI GGATG 1 cut(s) 883
BtsI GCAGTG 2 cut(s) 253, 1021
BtsIMutI CAGTG 2 cut(s) 253, 1021
Cac8I GCNNGC 2 cut(s) 211, 1188
CaiI CAGNNNCTG 1 cut(s) 290
CciI TCATGA 1 cut(s) 706
CfoI GCGC 2 cut(s) 54, 1186
Cfr13I GGNCC 5 cut(s) 4, 151, 421, 871, 1045
Csp6I GTAC 4 cut(s) 573, 632, 805, 1105
CviAII CATG 5 cut(s) 136, 707, 815, 830, 1160
CviQI GTAC 4 cut(s) 573, 632, 805, 1105
DdeI CTNAG 4 cut(s) 693, 762, 791, 1062
DpnI GATC 4 cut(s) 66, 705, 1020, 1164
DpnII GATC 4 cut(s) 64, 703, 1018, 1162
Eam1104I CTCTTC 2 cut(s) 82, 213
EarI CTCTTC 2 cut(s) 82, 213
Eco130I CCWWGG 1 cut(s) 186
Eco32I GATATC 1 cut(s) 1170
Eco47I GGWCC 2 cut(s) 421, 1045
Eco47III AGCGCT 1 cut(s) 53
Eco81I CCTNAGG 1 cut(s) 1062
EcoO109I RGGNCCY 2 cut(s) 421, 1045
EcoRI GAATTC 2 cut(s) 390, 860
EcoRII CCWGG 3 cut(s) 171, 289, 873
EcoRV GATATC 1 cut(s) 1170
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 5 cut(s) 139, 710, 818, 833, 1163
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FatI CATG 5 cut(s) 135, 706, 814, 829, 1159
FauI CCCGC 1 cut(s) 1179
FblI GTMKAC 3 cut(s) 11, 597, 846
Fnu4HI GCNGC 3 cut(s) 150, 164, 699
FokI GGATG 1 cut(s) 890
Fsp4HI GCNGC 3 cut(s) 150, 164, 699
FspBI CTAG 2 cut(s) 350, 1082
GlaI GCGC 2 cut(s) 53, 1185
GluI GCNGC 3 cut(s) 150, 164, 699
GsuI CTGGAG 1 cut(s) 477
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 4 cut(s) 5, 152, 294, 873
HhaI GCGC 2 cut(s) 54, 1186
Hin1II CATG 5 cut(s) 139, 710, 818, 833, 1163
Hin6I GCGC 2 cut(s) 52, 1184
HinP1I GCGC 2 cut(s) 52, 1184
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 2 cut(s) 754, 917
Hpy166II GTNNAC 4 cut(s) 12, 598, 847, 1079
Hpy188I TCNGA 5 cut(s) 265, 554, 866, 963, 1167
Hpy188III TCNNGA 8 cut(s) 62, 238, 494, 707, 971, 1000, 1061, 1173
Hpy8I GTNNAC 4 cut(s) 12, 598, 847, 1079
Hpy99I CGWCG 2 cut(s) 250, 367
HpyAV CCTTC 2 cut(s) 91, 418
HpyCH4III ACNGT 4 cut(s) 206, 286, 595, 623
HpyCH4IV ACGT 1 cut(s) 581
HpyCH4V TGCA 7 cut(s) 380, 515, 671, 701, 907, 938, 1026
HpyF3I CTNAG 4 cut(s) 693, 762, 791, 1062
HpySE526I ACGT 1 cut(s) 581
Hsp92II CATG 5 cut(s) 139, 710, 818, 833, 1163
HspAI GCGC 2 cut(s) 52, 1184
Kzo9I GATC 4 cut(s) 64, 703, 1018, 1162
LmnI GCTCC 3 cut(s) 496, 952, 1063
Lsp1109I GCAGC 2 cut(s) 175, 685
LweI GCATC 1 cut(s) 1045
MaeI CTAG 2 cut(s) 350, 1082
MaeII ACGT 1 cut(s) 581
MaeIII GTNAC 5 cut(s) 142, 358, 505, 562, 718
MalI GATC 4 cut(s) 66, 705, 1020, 1164
MboI GATC 4 cut(s) 64, 703, 1018, 1162
MboII GAAGA 3 cut(s) 69, 230, 1049
MfeI CAATTG 1 cut(s) 510
MhlI GDGCHC 2 cut(s) 211, 613
MluCI AATT 8 cut(s) 128, 306, 390, 510, 616, 735, 860, 1127
MlyI GAGTC 1 cut(s) 748
MmeI TCCRAC 1 cut(s) 41
MseI TTAA 3 cut(s) 23, 117, 426
MslI CAYNNNNRTG 1 cut(s) 945
MspR9I CCNGG 3 cut(s) 173, 291, 875
MunI CAATTG 1 cut(s) 510
MvaI CCWGG 3 cut(s) 173, 291, 875
MvnI CGCG 2 cut(s) 744, 1186
NdeII GATC 4 cut(s) 64, 703, 1018, 1162
NlaIII CATG 5 cut(s) 139, 710, 818, 833, 1163
NlaIV GGNNCC 4 cut(s) 177, 492, 883, 1152
NmuCI GTSAC 2 cut(s) 142, 562
NspI RCATGY 1 cut(s) 818
NspV TTCGAA 1 cut(s) 473
PagI TCATGA 1 cut(s) 706
PcsI WCGNNNNNNNCGW 1 cut(s) 1171
PfeI GAWTC 1 cut(s) 917
PkrI GCNGC 3 cut(s) 151, 165, 700
PleI GAGTC 1 cut(s) 748
PpsI GAGTC 1 cut(s) 748
PpuMI RGGWCCY 2 cut(s) 421, 1045
Psp5II RGGWCCY 2 cut(s) 421, 1045
Psp6I CCWGG 3 cut(s) 171, 289, 873
PspGI CCWGG 3 cut(s) 171, 289, 873
PspN4I GGNNCC 4 cut(s) 177, 492, 883, 1152
PspPI GGNCC 5 cut(s) 4, 151, 421, 871, 1045
PspPPI RGGWCCY 2 cut(s) 421, 1045
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 290
RsaI GTAC 4 cut(s) 574, 633, 806, 1106
RsaNI GTAC 4 cut(s) 573, 632, 805, 1105
RseI CAYNNNNRTG 1 cut(s) 945
SaqAI TTAA 3 cut(s) 23, 117, 426
SatI GCNGC 3 cut(s) 150, 164, 699
Sau3AI GATC 4 cut(s) 64, 703, 1018, 1162
Sau96I GGNCC 5 cut(s) 4, 151, 421, 871, 1045
ScaI AGTACT 1 cut(s) 574
SchI GAGTC 1 cut(s) 748
ScrFI CCNGG 3 cut(s) 173, 291, 875
SduI GDGCHC 2 cut(s) 211, 613
SfaNI GCATC 1 cut(s) 1045
SfcI CTRYAG 1 cut(s) 699
SfuI TTCGAA 1 cut(s) 473
SinI GGWCC 2 cut(s) 421, 1045
SmiMI CAYNNNNRTG 1 cut(s) 945
SmlI CTYRAG 3 cut(s) 47, 756, 1000
SmoI CTYRAG 3 cut(s) 47, 756, 1000
Sse9I AATT 8 cut(s) 128, 306, 390, 510, 616, 735, 860, 1127
SsiI CCGC 4 cut(s) 149, 179, 744, 1186
SspMI CTAG 2 cut(s) 350, 1082
StyD4I CCNGG 3 cut(s) 171, 289, 873
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 4 cut(s) 206, 286, 595, 623
TaiI ACGT 1 cut(s) 584
TaqI TCGA 3 cut(s) 473, 613, 1207
TasI AATT 8 cut(s) 128, 306, 390, 510, 616, 735, 860, 1127
TatI WGTACW 2 cut(s) 572, 631
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 1 cut(s) 917
Tru1I TTAA 3 cut(s) 23, 117, 426
Tru9I TTAA 3 cut(s) 23, 117, 426
TscAI CASTG 2 cut(s) 260, 1028
TseFI GTSAC 2 cut(s) 142, 562
TseI GCWGC 2 cut(s) 163, 698
Tsp45I GTSAC 2 cut(s) 142, 562
TspDTI ATGAA 3 cut(s) 513, 786, 873
TspGWI ACGGA 2 cut(s) 380, 822
TspRI CASTG 2 cut(s) 260, 1028
VpaK11BI GGWCC 2 cut(s) 421, 1045
XapI RAATTY 4 cut(s) 128, 390, 860, 1127
XceI RCATGY 1 cut(s) 818
XmiI GTMKAC 3 cut(s) 11, 597, 846
XspI CTAG 2 cut(s) 350, 1082
ZrmI AGTACT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.