RLG00000000972

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002383
Physical Location & Seq
Reverse (-)
8690367 .. 8695139
4773 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000972

Sequence Viewer

Length: 1179 bp
ATGGGTCAGTATACCCAAACCTTAAACCCTCCCAAAACCCTCATCCCCAAGCTCCCAAATCCCAACCCCTCTTTCTCCTCCTTCAAAACCCCAAAACCCAAATCCAAAAGCTTCCCAACACTCTCGGTCTCGGCCATGGCGGCCCAAAATCAACCGCCCCTGGATGCTCTGCTCAACGGTGCTCGCAAACAAGAGTTGCTTGGCGCCATTGGGACTTCATTGTCTAACTGTCTTTCTGAAACCAACCTCCACCTCACAGTTCCAGGCCTCAAATCCAAAATCAGAGGCAAGGTTAGGGATGTTTATGACAGTGGGGATTATCTAGTTATGGTCACCACAGATCGGCAGAGTGCATTTGATAGAATTCTTGCTTCCATTCCCTTTAAAGGCCAGGTCCTTAATGAAACGAGTTTATGGTGGTTTGACAAAACTCAACATATAACGTCAAATGCAGTTGTATCAGTCCCAGACGAAAATGTAACAATTGTAAAGAAATGTTCTGTTTTTCCGGTGGAGTTTGTGGTCAGAGGTTTTGTGACTGGAAGTACTGATACATCATTATGGACGGTCTACAGAAATGGCATTCGAAATTACTGTGGCAATGTACTTCCAGATGGCTTGGTAAAAAGCCAAAAGCTTCCTGCAAATATACTCACACCAACAACTAAGGCTGCAGATCATGATGTTCCTGTTACTCCAGATGAGATAATTGAACGTGGACTGATGAGTCAAGCCGATTATGATGAAGTAAGTCAGAAAGCATTAAGCTTGTTCGAGTATGGACAGCGCGTGGCTTTGGAGCATGGGTTGATATTGGTAGACACAAAGTATGAATTTGGAAAGGGACAGGATGGTTCCATACATTTGATTGATGAGGTGCATACGCCTGATTCAAGCAGATATTGGATTGCACATACTTATGGGGAACGTTTTCGAAATGGTCTTGAACCTGAGAATGTTGATAAGGAGTTCTTGAGGTTGTGGTTTAAAGATCATTGCAATCCATATGAAGATGAGGTCCTCCCTGATGCCCCTGAAGAACTTGTTCGTGAACTATCTTGGCGATATATTTTCTTGTATGAGACAATTACAAAATCAAAATTCAAGATGCCTTCAATAGAGGAGCCAATACATGATCGAATATCTCGCAATGTGGCACAAGCACTGTCATCTCTATAA

Protein Analysis

393

Amino Acids

44.06

Weight (kDa)

6.03

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAICAR_synt PF01259 94 - 339 1.8e-68 SAICAR synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 220, 726
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 3 cut(s) 11, 570, 819
AccII CGCG 1 cut(s) 789
AciI CCGC 2 cut(s) 140, 155
AclI AACGTT 1 cut(s) 928
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 3 cut(s) 363, 833, 1100
AcuI CTGAAG 1 cut(s) 1056
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 2 cut(s) 547, 606
AfiI CCNNNNNNNGG 2 cut(s) 342, 386
AgsI TTSAA 6 cut(s) 85, 713, 894, 947, 1105, 1116
AjnI CCWGG 3 cut(s) 159, 262, 390
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 4 cut(s) 52, 111, 637, 768
AluI AGCT 4 cut(s) 52, 111, 637, 768
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 133, 1076
AoxI GGCC 4 cut(s) 132, 141, 265, 388
ApeKI GCWGC 1 cut(s) 671
ApoI RAATTY 3 cut(s) 363, 833, 1100
Asp700I GAANNNNTTC 2 cut(s) 930, 1044
AspLEI GCGC 2 cut(s) 206, 789
AspS9I GGNCC 3 cut(s) 142, 394, 1018
AsuHPI GGTGA 1 cut(s) 325
AsuII TTCGAA 2 cut(s) 586, 934
AvaII GGWCC 2 cut(s) 394, 1018
BanI GGYRCC 1 cut(s) 203
BarI GAAGNNNNNNTAC 2 cut(s) 535, 567
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 658
BccI CCATC 2 cut(s) 608, 845
BciT130I CCWGG 3 cut(s) 161, 264, 392
BcoDI GTCTC 2 cut(s) 133, 1076
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 2 cut(s) 571, 672
BfoI RGCGCY 1 cut(s) 207
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 2 cut(s) 141, 672
BlsI GCNGC 2 cut(s) 142, 673
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 3 cut(s) 161, 264, 392
Bme18I GGWCC 2 cut(s) 394, 1018
BmgT120I GGNCC 3 cut(s) 142, 394, 1018
BmiI GGNNCC 3 cut(s) 205, 856, 1125
BmrFI CCNGG 3 cut(s) 161, 264, 392
BmsI GCATC 3 cut(s) 154, 1018, 1098
BpmI CTGGAG 1 cut(s) 681
Bpu14I TTCGAA 2 cut(s) 586, 934
BpuEI CTTGAG 1 cut(s) 994
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 135, 159
BsaWI WCCGGW 1 cut(s) 508
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 386
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 3 cut(s) 607, 994, 1156
BseBI CCWGG 3 cut(s) 161, 264, 392
BseDI CCNNGG 2 cut(s) 135, 159
BseGI GGATG 4 cut(s) 42, 169, 304, 856
BseLI CCNNNNNNNGG 2 cut(s) 342, 386
BseMI GCAATG 3 cut(s) 607, 994, 1156
BseMII CTCAG 1 cut(s) 942
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 2 cut(s) 67, 1136
BseXI GCAGC 1 cut(s) 658
Bsh1236I CGCG 1 cut(s) 789
BshFI GGCC 4 cut(s) 134, 143, 267, 390
BshNI GGYRCC 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 1 cut(s) 509
BslFI GGGAC 3 cut(s) 226, 449, 858
BslI CCNNNNNNNGG 2 cut(s) 342, 386
BsmAI GTCTC 2 cut(s) 133, 1076
BsmFI GGGAC 3 cut(s) 226, 449, 858
BsmI GAATGC 1 cut(s) 582
BsnI GGCC 4 cut(s) 134, 143, 267, 390
Bso31I GGTCTC 1 cut(s) 133
Bsp119I TTCGAA 2 cut(s) 586, 934
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 4 cut(s) 340, 676, 991, 1135
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 2 cut(s) 140, 155
BspANI GGCC 4 cut(s) 134, 143, 267, 390
BspCNI CTCAG 1 cut(s) 943
BspFNI CGCG 1 cut(s) 789
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 3 cut(s) 205, 856, 1125
BspMAI CTGCAG 1 cut(s) 676
BspT104I TTCGAA 2 cut(s) 586, 934
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 3 cut(s) 607, 994, 1156
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 2 cut(s) 135, 159
BssMI GATC 4 cut(s) 340, 676, 991, 1135
BssNAI GTATAC 1 cut(s) 12
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 135
Bst1107I GTATAC 1 cut(s) 12
Bst2UI CCWGG 3 cut(s) 161, 264, 392
Bst4CI ACNGT 7 cut(s) 179, 230, 259, 311, 568, 596, 1167
BstACI GRCGYC 1 cut(s) 204
BstBI TTCGAA 2 cut(s) 586, 934
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 2 cut(s) 666, 951
BstDSI CCRYGG 1 cut(s) 135
BstEII GGTNACC 1 cut(s) 331
BstF5I GGATG 4 cut(s) 42, 169, 304, 856
BstFNI CGCG 1 cut(s) 789
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 2 cut(s) 206, 789
BstKTI GATC 4 cut(s) 343, 679, 994, 1138
BstMAI GTCTC 2 cut(s) 133, 1076
BstMBI GATC 4 cut(s) 340, 676, 991, 1135
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNI CCWGG 3 cut(s) 161, 264, 392
BstPI GGTNACC 1 cut(s) 331
BstSCI CCNGG 3 cut(s) 159, 262, 390
BstSFI CTRYAG 2 cut(s) 571, 672
BstUI CGCG 1 cut(s) 789
BstV1I GCAGC 1 cut(s) 658
BstZ17I GTATAC 1 cut(s) 12
BsuRI GGCC 4 cut(s) 134, 143, 267, 390
BtgI CCRYGG 1 cut(s) 135
BtsCI GGATG 4 cut(s) 42, 169, 304, 856
BtsIMutI CAGTG 2 cut(s) 316, 1163
Cac8I GCNNGC 1 cut(s) 184
CciI TCATGA 1 cut(s) 679
CfoI GCGC 2 cut(s) 206, 789
Cfr13I GGNCC 3 cut(s) 142, 394, 1018
Csp6I GTAC 2 cut(s) 546, 605
CviAII CATG 4 cut(s) 136, 680, 803, 1133
CviQI GTAC 2 cut(s) 546, 605
DdeI CTNAG 2 cut(s) 666, 951
DinI GGCGCC 1 cut(s) 205
DpnI GATC 4 cut(s) 342, 678, 993, 1137
DpnII GATC 4 cut(s) 340, 676, 991, 1135
DraI TTTAAA 2 cut(s) 385, 988
DrdI GACNNNNNNGTC 2 cut(s) 220, 726
DseDI GACNNNNNNGTC 2 cut(s) 220, 726
EaeI YGGCCR 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 135
Eco147I AGGCCT 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 133
Eco47I GGWCC 2 cut(s) 394, 1018
Eco57I CTGAAG 1 cut(s) 1056
Eco91I GGTNACC 1 cut(s) 331
EcoO109I RGGNCCY 2 cut(s) 394, 1018
EcoO65I GGTNACC 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 363
EcoRII CCWGG 3 cut(s) 159, 262, 390
EcoT14I CCWWGG 1 cut(s) 135
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 135
FaeI CATG 4 cut(s) 139, 683, 806, 1136
FalI AAGNNNNNCTT 4 cut(s) 183, 215, 956, 988
FaqI GGGAC 3 cut(s) 226, 449, 858
FatI CATG 4 cut(s) 135, 679, 802, 1132
FauNDI CATATG 1 cut(s) 1006
FblI GTMKAC 3 cut(s) 11, 570, 819
Fnu4HI GCNGC 2 cut(s) 141, 672
FokI GGATG 4 cut(s) 29, 176, 311, 863
Fsp4HI GCNGC 2 cut(s) 141, 672
FspBI CTAG 1 cut(s) 323
GlaI GCGC 2 cut(s) 205, 788
GluI GCNGC 2 cut(s) 141, 672
GsuI CTGGAG 1 cut(s) 681
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 4 cut(s) 134, 143, 267, 390
HapII CCGG 1 cut(s) 509
HhaI GCGC 2 cut(s) 206, 789
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 4 cut(s) 139, 683, 806, 1136
Hin6I GCGC 2 cut(s) 204, 787
HinP1I GCGC 2 cut(s) 204, 787
HindIII AAGCTT 3 cut(s) 109, 635, 766
HinfI GANTC 2 cut(s) 727, 890
HpaII CCGG 1 cut(s) 509
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 5 cut(s) 12, 571, 719, 820, 1052
Hpy188I TCNGA 4 cut(s) 238, 284, 527, 756
Hpy188III TCNNGA 7 cut(s) 611, 680, 698, 944, 973, 1049, 1105
Hpy8I GTNNAC 5 cut(s) 12, 571, 719, 820, 1052
HpyAV CCTTC 2 cut(s) 91, 1122
HpyCH4III ACNGT 7 cut(s) 179, 230, 259, 311, 568, 596, 1167
HpyCH4IV ACGT 3 cut(s) 443, 715, 928
HpyCH4V TGCA 7 cut(s) 353, 452, 644, 674, 880, 911, 999
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 2 cut(s) 666, 951
HpySE526I ACGT 3 cut(s) 443, 715, 928
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 4 cut(s) 139, 683, 806, 1136
HspAI GCGC 2 cut(s) 204, 787
KasI GGCGCC 1 cut(s) 203
Kzo9I GATC 4 cut(s) 340, 676, 991, 1135
LmnI GCTCC 3 cut(s) 57, 799, 1123
Lsp1109I GCAGC 1 cut(s) 658
LweI GCATC 3 cut(s) 154, 1018, 1098
MaeI CTAG 1 cut(s) 323
MaeII ACGT 3 cut(s) 443, 715, 928
MaeIII GTNAC 4 cut(s) 331, 478, 535, 691
MalI GATC 4 cut(s) 342, 678, 993, 1137
MboI GATC 4 cut(s) 340, 676, 991, 1135
MboII GAAGA 2 cut(s) 1022, 1049
MfeI CAATTG 1 cut(s) 483
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 7 cut(s) 363, 483, 589, 708, 833, 1086, 1100
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 1 cut(s) 736
MroXI GAANNNNTTC 2 cut(s) 930, 1044
MseI TTAA 5 cut(s) 23, 384, 399, 764, 987
MslI CAYNNNNRTG 2 cut(s) 559, 918
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 3 cut(s) 161, 264, 392
MunI CAATTG 1 cut(s) 483
Mva1269I GAATGC 1 cut(s) 582
MvaI CCWGG 3 cut(s) 161, 264, 392
MvnI CGCG 1 cut(s) 789
MwoI GCNNNNNNNGC 1 cut(s) 140
NarI GGCGCC 1 cut(s) 204
NcoI CCATGG 1 cut(s) 135
NdeI CATATG 1 cut(s) 1006
NdeII GATC 4 cut(s) 340, 676, 991, 1135
NlaIII CATG 4 cut(s) 139, 683, 806, 1136
NlaIV GGNNCC 3 cut(s) 205, 856, 1125
NmeAIII GCCGAG 1 cut(s) 110
NmuCI GTSAC 2 cut(s) 331, 535
NspV TTCGAA 2 cut(s) 586, 934
PagI TCATGA 1 cut(s) 679
PceI AGGCCT 1 cut(s) 267
PctI GAATGC 1 cut(s) 582
PdmI GAANNNNTTC 2 cut(s) 930, 1044
PfeI GAWTC 1 cut(s) 890
PkrI GCNGC 2 cut(s) 142, 673
PleI GAGTC 1 cut(s) 735
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 735
PpuMI RGGWCCY 2 cut(s) 394, 1018
Psp1406I AACGTT 1 cut(s) 928
Psp5II RGGWCCY 2 cut(s) 394, 1018
Psp6I CCWGG 3 cut(s) 159, 262, 390
PspEI GGTNACC 1 cut(s) 331
PspGI CCWGG 3 cut(s) 159, 262, 390
PspN4I GGNNCC 3 cut(s) 205, 856, 1125
PspPI GGNCC 3 cut(s) 142, 394, 1018
PspPPI RGGWCCY 2 cut(s) 394, 1018
PstI CTGCAG 1 cut(s) 676
RsaI GTAC 2 cut(s) 547, 606
RsaNI GTAC 2 cut(s) 546, 605
RseI CAYNNNNRTG 2 cut(s) 559, 918
SaqAI TTAA 5 cut(s) 23, 384, 399, 764, 987
SatI GCNGC 2 cut(s) 141, 672
Sau3AI GATC 4 cut(s) 340, 676, 991, 1135
Sau96I GGNCC 3 cut(s) 142, 394, 1018
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 1 cut(s) 736
ScrFI CCNGG 3 cut(s) 161, 264, 392
SduI GDGCHC 1 cut(s) 184
SfaNI GCATC 3 cut(s) 154, 1018, 1098
SfcI CTRYAG 2 cut(s) 571, 672
SfiI GGCCNNNNNGGCC 1 cut(s) 140
SfoI GGCGCC 1 cut(s) 205
SfuI TTCGAA 2 cut(s) 586, 934
SinI GGWCC 2 cut(s) 394, 1018
SmiMI CAYNNNNRTG 2 cut(s) 559, 918
SmlI CTYRAG 1 cut(s) 973
SmoI CTYRAG 1 cut(s) 973
Sse9I AATT 7 cut(s) 363, 483, 589, 708, 833, 1086, 1100
SseBI AGGCCT 1 cut(s) 267
SsiI CCGC 2 cut(s) 140, 155
SspDI GGCGCC 1 cut(s) 203
SspMI CTAG 1 cut(s) 323
StuI AGGCCT 1 cut(s) 267
StyD4I CCNGG 3 cut(s) 159, 262, 390
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 7 cut(s) 179, 230, 259, 311, 568, 596, 1167
TaiI ACGT 3 cut(s) 446, 718, 931
TaqI TCGA 4 cut(s) 586, 774, 934, 1138
TaqII GACCGA 1 cut(s) 115
TasI AATT 7 cut(s) 363, 483, 589, 708, 833, 1086, 1100
TatI WGTACW 2 cut(s) 545, 604
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 890
Tru1I TTAA 5 cut(s) 23, 384, 399, 764, 987
Tru9I TTAA 5 cut(s) 23, 384, 399, 764, 987
TscAI CASTG 2 cut(s) 316, 1170
TseFI GTSAC 2 cut(s) 331, 535
TseI GCWGC 1 cut(s) 671
Tsp45I GTSAC 2 cut(s) 331, 535
TspDTI ATGAA 5 cut(s) 207, 417, 759, 846, 1023
TspRI CASTG 2 cut(s) 316, 1170
VpaK11BI GGWCC 2 cut(s) 394, 1018
XapI RAATTY 3 cut(s) 363, 833, 1100
XmiI GTMKAC 3 cut(s) 11, 570, 819
XmnI GAANNNNTTC 2 cut(s) 930, 1044
XspI CTAG 1 cut(s) 323
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.