RLG00000029972

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002383
Physical Location & Seq
Forward (+)
52467467 .. 52473257
5791 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029972

Sequence Viewer

Length: 1185 bp
ATGGCGGCTCAGTATACCCAAACCTTAAACCCTAAACCCAAAACCCTCATCCCCAAGCTCCCAAATCCCAACCCCTCTTTCTCCTCCTTCAAAACCCCAAAACCCAAATCCAAAAGCTTCTCAACACTCTCTGTCTCGGCCGTGGCGGCCCAAAATCAACCGCCTCTGGATACTCTGCTCAACGGTGCTCGCAAACAAGAGGTGCTTGGCGCCATTGGGACTTCATTGTCTAACTGTCTTTCCGAAACCAACCTCCACCTCACAGTTCCTGGCCTGAAATCCAAAACCAGAGGCAAGGTTAGGGATGTTTACGACAGTGGGGATTATCTAGTTATGGTCACCACGGATCGGCAGAGTGCATTTGATAGAATTCTTGCTTCCATTCCCTTTAAAGGCCAGGTCCTTAATGAAACAAGTTTATGGTGGTTTGACAAAACTCAACATATAACGTCAAATGCTGTTGTATCGGTCCCAGATGAAAATGTAACAATTGCAAAGAAATGTTCTGTTTTCCCGGTGGAGTTTGTGGTCAGAGGTTTTGTGACTGGAAGTACTGATACATCATTATGGACGGTCTATAGAAGTGGTGTTCAAAATTACTGTGGCAATGTACTTCCAGACGGCTTGGTAAAAAGCCAAAAGCTTCCTGCAAATATACTCACACCAACAACTAAGGCTGCAGATCATGATGTTCCTGTTACTCCAGATGAGATAATTGAACGTGGACTGATGAGTCGAGCCGATTATGATGAAGTAAGTCGGAAAGCATTAAGCTTGTTTGAGTATGGTCAGCGTGTGGCTTTGGAGCATGGGTTGATATTGGTAGACACAAAGTATGAATTTGGAAAGGGACAGGATGGTTCCATACATTTGATTGATGAGGTGCATACACCTGATTCAAGTAGATATTGGATTGCACATACTTATGAGGAACGTTTTCAGAATGGTCTTGAGCCTGAGAATGTTGATAAGGAGTTCTTGAGGTTGTGGTTTAAAGATCATTGCAATCCATATGAAGATGAGGTCCTCCCTGATGCCCCTGAAGAACTTGTTCGTGAACTATCTTGGCGATATATTTTCTTGTATGAGACAATTACAAAATCAAAATTCAAGATGCCTTCAACAGAGGAGCCAATACATGATCGAATATCTCGCAATGTGGCACAAGCACTGTCATCTCTGTAA

Protein Analysis

395

Amino Acids

44.23

Weight (kDa)

6.03

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAICAR_synt PF01259 96 - 341 2.5e-68 SAICAR synthetase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 226, 732
AccB1I GGYRCC 1 cut(s) 209
AccI GTMKAC 2 cut(s) 14, 825
AciI CCGC 3 cut(s) 5, 146, 161
AclI AACGTT 1 cut(s) 934
AclWI GGATC 1 cut(s) 354
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 3 cut(s) 369, 839, 1106
AcuI CTGAAG 1 cut(s) 1062
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 2 cut(s) 553, 612
AfiI CCNNNNNNNGG 2 cut(s) 348, 392
AgsI TTSAA 6 cut(s) 91, 593, 719, 900, 1111, 1122
AjnI CCWGG 2 cut(s) 268, 396
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AluBI AGCT 4 cut(s) 58, 117, 643, 774
AluI AGCT 4 cut(s) 58, 117, 643, 774
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 2 cut(s) 139, 1082
AlwI GGATC 1 cut(s) 354
AlwNI CAGNNNCTG 1 cut(s) 269
AoxI GGCC 4 cut(s) 138, 147, 271, 394
ApeKI GCWGC 1 cut(s) 677
ApoI RAATTY 3 cut(s) 369, 839, 1106
Asp700I GAANNNNTTC 2 cut(s) 936, 1050
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 4 cut(s) 148, 400, 469, 1024
AsuC2I CCSGG 1 cut(s) 515
AsuHPI GGTGA 1 cut(s) 331
AvaII GGWCC 3 cut(s) 400, 469, 1024
BanI GGYRCC 1 cut(s) 209
BarI GAAGNNNNNNTAC 2 cut(s) 541, 573
Bbv12I GWGCWC 1 cut(s) 190
BbvI GCAGC 1 cut(s) 664
BccI CCATC 1 cut(s) 851
BceAI ACGGC 2 cut(s) 125, 637
BciT130I CCWGG 2 cut(s) 270, 398
BciVI GTATCC 1 cut(s) 163
BcnI CCSGG 1 cut(s) 515
BcoDI GTCTC 2 cut(s) 139, 1082
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 2 cut(s) 577, 678
BfoI RGCGCY 1 cut(s) 213
BfuI GTATCC 1 cut(s) 163
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 3 cut(s) 6, 147, 678
BlsI GCNGC 3 cut(s) 7, 148, 679
BmcAI AGTACT 1 cut(s) 553
Bme1390I CCNGG 3 cut(s) 270, 398, 515
Bme18I GGWCC 3 cut(s) 400, 469, 1024
BmgT120I GGNCC 4 cut(s) 148, 400, 469, 1024
BmiI GGNNCC 4 cut(s) 211, 471, 862, 1131
BmrFI CCNGG 3 cut(s) 270, 398, 515
BmsI GCATC 2 cut(s) 1024, 1104
BpmI CTGGAG 1 cut(s) 687
BpuEI CTTGAG 2 cut(s) 971, 1000
BpuMI CCSGG 1 cut(s) 515
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 2 cut(s) 141, 342
BsaXI ACNNNNNCTCC 2 cut(s) 797, 827
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 392
Bse1I ACTGG 1 cut(s) 550
Bse3DI GCAATG 3 cut(s) 613, 1000, 1162
BseBI CCWGG 2 cut(s) 270, 398
BseDI CCNNGG 2 cut(s) 141, 342
BseGI GGATG 3 cut(s) 48, 310, 862
BseLI CCNNNNNNNGG 2 cut(s) 348, 392
BseMI GCAATG 3 cut(s) 613, 1000, 1162
BseMII CTCAG 2 cut(s) 23, 948
BseNI ACTGG 1 cut(s) 550
BseRI GAGGAG 2 cut(s) 73, 1142
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 1 cut(s) 664
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 4 cut(s) 140, 149, 273, 396
BshNI GGYRCC 1 cut(s) 209
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 190
BsiSI CCGG 1 cut(s) 515
BslFI GGGAC 3 cut(s) 232, 455, 864
BslI CCNNNNNNNGG 2 cut(s) 348, 392
BsmAI GTCTC 2 cut(s) 139, 1082
BsmFI GGGAC 3 cut(s) 232, 455, 864
BsnI GGCC 4 cut(s) 140, 149, 273, 396
Bsp1286I GDGCHC 1 cut(s) 190
Bsp143I GATC 4 cut(s) 346, 682, 997, 1141
BspACI CCGC 3 cut(s) 5, 146, 161
BspANI GGCC 4 cut(s) 140, 149, 273, 396
BspCNI CTCAG 2 cut(s) 22, 949
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 4 cut(s) 211, 471, 862, 1131
BspMAI CTGCAG 1 cut(s) 682
BspPI GGATC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 209
BsrDI GCAATG 3 cut(s) 613, 1000, 1162
BsrI ACTGG 1 cut(s) 550
BssECI CCNNGG 2 cut(s) 141, 342
BssMI GATC 4 cut(s) 346, 682, 997, 1141
BssNAI GTATAC 1 cut(s) 15
BssNI GRCGYC 1 cut(s) 210
Bst1107I GTATAC 1 cut(s) 15
Bst2UI CCWGG 2 cut(s) 270, 398
Bst4CI ACNGT 7 cut(s) 185, 236, 265, 317, 574, 602, 1173
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 3 cut(s) 9, 672, 957
BstDSI CCRYGG 2 cut(s) 141, 342
BstEII GGTNACC 1 cut(s) 337
BstF5I GGATG 3 cut(s) 48, 310, 862
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 4 cut(s) 349, 685, 1000, 1144
BstMAI GTCTC 2 cut(s) 139, 1082
BstMBI GATC 4 cut(s) 346, 682, 997, 1141
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 2 cut(s) 270, 398
BstPI GGTNACC 1 cut(s) 337
BstSCI CCNGG 3 cut(s) 268, 396, 513
BstSFI CTRYAG 2 cut(s) 577, 678
BstV1I GCAGC 1 cut(s) 664
BstZ17I GTATAC 1 cut(s) 15
BstZI CGGCCG 1 cut(s) 138
BsuI GTATCC 1 cut(s) 163
BsuRI GGCC 4 cut(s) 140, 149, 273, 396
BtgI CCRYGG 2 cut(s) 141, 342
BtsCI GGATG 3 cut(s) 48, 310, 862
BtsIMutI CAGTG 2 cut(s) 322, 1169
Cac8I GCNNGC 1 cut(s) 190
CaiI CAGNNNCTG 1 cut(s) 269
CciI TCATGA 1 cut(s) 685
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 4 cut(s) 148, 400, 469, 1024
Csp6I GTAC 2 cut(s) 552, 611
CviAII CATG 3 cut(s) 686, 809, 1139
CviQI GTAC 2 cut(s) 552, 611
DdeI CTNAG 3 cut(s) 9, 672, 957
DinI GGCGCC 1 cut(s) 211
DpnI GATC 4 cut(s) 348, 684, 999, 1143
DpnII GATC 4 cut(s) 346, 682, 997, 1141
DraI TTTAAA 2 cut(s) 391, 994
DrdI GACNNNNNNGTC 2 cut(s) 226, 732
DseDI GACNNNNNNGTC 2 cut(s) 226, 732
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
EclXI CGGCCG 1 cut(s) 138
Eco47I GGWCC 3 cut(s) 400, 469, 1024
Eco52I CGGCCG 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 1062
Eco91I GGTNACC 1 cut(s) 337
EcoO109I RGGNCCY 2 cut(s) 400, 1024
EcoO65I GGTNACC 1 cut(s) 337
EcoRI GAATTC 1 cut(s) 369
EcoRII CCWGG 2 cut(s) 268, 396
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
FaeI CATG 3 cut(s) 689, 812, 1142
FalI AAGNNNNNCTT 4 cut(s) 189, 221, 962, 994
FaqI GGGAC 3 cut(s) 232, 455, 864
FatI CATG 3 cut(s) 685, 808, 1138
FauNDI CATATG 1 cut(s) 1012
FblI GTMKAC 2 cut(s) 14, 825
Fnu4HI GCNGC 3 cut(s) 6, 147, 678
FokI GGATG 3 cut(s) 35, 317, 869
Fsp4HI GCNGC 3 cut(s) 6, 147, 678
FspBI CTAG 1 cut(s) 329
GlaI GCGC 1 cut(s) 211
GluI GCNGC 3 cut(s) 6, 147, 678
GsuI CTGGAG 1 cut(s) 687
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 4 cut(s) 140, 149, 273, 396
HapII CCGG 1 cut(s) 515
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 3 cut(s) 689, 812, 1142
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 3 cut(s) 115, 641, 772
HinfI GANTC 2 cut(s) 733, 896
HpaII CCGG 1 cut(s) 515
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 5 cut(s) 15, 310, 725, 826, 1058
Hpy188I TCNGA 4 cut(s) 244, 533, 762, 942
Hpy188III TCNNGA 8 cut(s) 167, 617, 686, 704, 950, 979, 1055, 1111
Hpy8I GTNNAC 5 cut(s) 15, 310, 725, 826, 1058
HpyAV CCTTC 2 cut(s) 97, 1128
HpyCH4III ACNGT 7 cut(s) 185, 236, 265, 317, 574, 602, 1173
HpyCH4IV ACGT 3 cut(s) 449, 721, 934
HpyCH4V TGCA 7 cut(s) 359, 494, 650, 680, 886, 917, 1005
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 3 cut(s) 9, 672, 957
HpySE526I ACGT 3 cut(s) 449, 721, 934
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 3 cut(s) 689, 812, 1142
HspAI GCGC 1 cut(s) 210
KasI GGCGCC 1 cut(s) 209
Kzo9I GATC 4 cut(s) 346, 682, 997, 1141
LmnI GCTCC 3 cut(s) 63, 805, 1129
Lsp1109I GCAGC 1 cut(s) 664
LweI GCATC 2 cut(s) 1024, 1104
MaeI CTAG 1 cut(s) 329
MaeII ACGT 3 cut(s) 449, 721, 934
MaeIII GTNAC 4 cut(s) 337, 484, 541, 697
MalI GATC 4 cut(s) 348, 684, 999, 1143
MboI GATC 4 cut(s) 346, 682, 997, 1141
MboII GAAGA 2 cut(s) 1028, 1055
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 7 cut(s) 369, 489, 595, 714, 839, 1092, 1106
Mly113I GGCGCC 1 cut(s) 210
MlyI GAGTC 1 cut(s) 742
MmeI TCCRAC 1 cut(s) 740
MroXI GAANNNNTTC 2 cut(s) 936, 1050
MseI TTAA 5 cut(s) 26, 390, 405, 770, 993
MslI CAYNNNNRTG 2 cut(s) 565, 924
MspI CCGG 1 cut(s) 515
MspR9I CCNGG 3 cut(s) 270, 398, 515
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 2 cut(s) 270, 398
MwoI GCNNNNNNNGC 1 cut(s) 146
NarI GGCGCC 1 cut(s) 210
NciI CCSGG 1 cut(s) 515
NdeI CATATG 1 cut(s) 1012
NdeII GATC 4 cut(s) 346, 682, 997, 1141
NlaIII CATG 3 cut(s) 689, 812, 1142
NlaIV GGNNCC 4 cut(s) 211, 471, 862, 1131
NmeAIII GCCGAG 1 cut(s) 116
NmuCI GTSAC 2 cut(s) 337, 541
PagI TCATGA 1 cut(s) 685
PdmI GAANNNNTTC 2 cut(s) 936, 1050
PfeI GAWTC 1 cut(s) 896
PkrI GCNGC 3 cut(s) 7, 148, 679
PleI GAGTC 1 cut(s) 741
PluTI GGCGCC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 741
PpuMI RGGWCCY 2 cut(s) 400, 1024
Psp1406I AACGTT 1 cut(s) 934
Psp5II RGGWCCY 2 cut(s) 400, 1024
Psp6I CCWGG 2 cut(s) 268, 396
PspEI GGTNACC 1 cut(s) 337
PspGI CCWGG 2 cut(s) 268, 396
PspN4I GGNNCC 4 cut(s) 211, 471, 862, 1131
PspPI GGNCC 4 cut(s) 148, 400, 469, 1024
PspPPI RGGWCCY 2 cut(s) 400, 1024
PstI CTGCAG 1 cut(s) 682
PstNI CAGNNNCTG 1 cut(s) 269
RsaI GTAC 2 cut(s) 553, 612
RsaNI GTAC 2 cut(s) 552, 611
RseI CAYNNNNRTG 2 cut(s) 565, 924
SaqAI TTAA 5 cut(s) 26, 390, 405, 770, 993
SatI GCNGC 3 cut(s) 6, 147, 678
Sau3AI GATC 4 cut(s) 346, 682, 997, 1141
Sau96I GGNCC 4 cut(s) 148, 400, 469, 1024
ScaI AGTACT 1 cut(s) 553
SchI GAGTC 1 cut(s) 742
ScrFI CCNGG 3 cut(s) 270, 398, 515
SduI GDGCHC 1 cut(s) 190
SfaNI GCATC 2 cut(s) 1024, 1104
SfcI CTRYAG 2 cut(s) 577, 678
SfiI GGCCNNNNNGGCC 1 cut(s) 146
SfoI GGCGCC 1 cut(s) 211
SinI GGWCC 3 cut(s) 400, 469, 1024
SmiMI CAYNNNNRTG 2 cut(s) 565, 924
SmlI CTYRAG 2 cut(s) 950, 979
SmoI CTYRAG 2 cut(s) 950, 979
Sse9I AATT 7 cut(s) 369, 489, 595, 714, 839, 1092, 1106
SsiI CCGC 3 cut(s) 5, 146, 161
SspDI GGCGCC 1 cut(s) 209
SspMI CTAG 1 cut(s) 329
StyD4I CCNGG 3 cut(s) 268, 396, 513
TaaI ACNGT 7 cut(s) 185, 236, 265, 317, 574, 602, 1173
TaiI ACGT 3 cut(s) 452, 724, 937
TaqI TCGA 2 cut(s) 736, 1144
TaqII GACCGA 1 cut(s) 457
TasI AATT 7 cut(s) 369, 489, 595, 714, 839, 1092, 1106
TatI WGTACW 2 cut(s) 551, 610
TauI GCSGC 2 cut(s) 8, 149
TfiI GAWTC 1 cut(s) 896
Tru1I TTAA 5 cut(s) 26, 390, 405, 770, 993
Tru9I TTAA 5 cut(s) 26, 390, 405, 770, 993
TscAI CASTG 2 cut(s) 322, 1176
TseFI GTSAC 2 cut(s) 337, 541
TseI GCWGC 1 cut(s) 677
Tsp45I GTSAC 2 cut(s) 337, 541
TspDTI ATGAA 6 cut(s) 213, 423, 492, 765, 852, 1029
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 2 cut(s) 322, 1176
VpaK11BI GGWCC 3 cut(s) 400, 469, 1024
XapI RAATTY 3 cut(s) 369, 839, 1106
XmiI GTMKAC 2 cut(s) 14, 825
XmnI GAANNNNTTC 2 cut(s) 936, 1050
XspI CTAG 1 cut(s) 329
ZrmI AGTACT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.