MD16G1221600.v1.1

Polyubiquitin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005187
Physical Location & Seq
Reverse (-)
22135538 .. 22135786
249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1221600.v1.1.491

Sequence Viewer

Length: 249 bp
ATGTGGAGTGGGAAAACCATTACACTTGACGTGACAGGAAAGAGCACTGTCAAAGAGGTGAAGGACAAGATATTTTGCAAGGTTAGAGTGCCAGTTCGTGTTCAGAGTATTGTAGTTGCCGGTAAACGCCTTGATGATGAATGCTGTCTTTCAAGTTACAACATTCAGAAAGGCTCCACTCTCCACATGGTTTTGGGTTGGCGAACCAAATTCCCTTATCATTCGTCATTCACAAGAAATTCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

83

Amino Acids

9.35

Weight (kDa)

9.96

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 2 - 65 2e-18 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 209, 238
AgsI TTSAA 2 cut(s) 153, 243
AjiI CACGTC 1 cut(s) 31
Alw21I GWGCWC 1 cut(s) 47
ApoI RAATTY 2 cut(s) 209, 238
AsuHPI GGTGA 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 31
BmiI GGNNCC 1 cut(s) 175
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 1 cut(s) 120
BsmI GAATGC 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 47
BspLI GGNNCC 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 119
Bst4CI ACNGT 1 cut(s) 49
BtrI CACGTC 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 119
CviAII CATG 1 cut(s) 187
CviJI RGCY 1 cut(s) 174
CviKI_1 RGCY 1 cut(s) 174
FaeI CATG 1 cut(s) 190
FaiI YATR 1 cut(s) 188
FatI CATG 1 cut(s) 186
HapII CCGG 1 cut(s) 120
Hin1II CATG 1 cut(s) 190
HpaII CCGG 1 cut(s) 120
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 1 cut(s) 125
Hpy188I TCNGA 2 cut(s) 105, 168
Hpy188III TCNNGA 1 cut(s) 243
Hpy8I GTNNAC 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 1 cut(s) 78
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 1 cut(s) 190
LmnI GCTCC 1 cut(s) 179
LpnPI CCDG 3 cut(s) 21, 105, 133
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 2 cut(s) 31, 155
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 2 cut(s) 209, 238
MnlI CCTC 1 cut(s) 49
MspI CCGG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 146
NlaIII CATG 1 cut(s) 190
NlaIV GGNNCC 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 31
PctI GAATGC 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 175
SduI GDGCHC 1 cut(s) 47
SetI ASST 3 cut(s) 33, 60, 84
Sse9I AATT 2 cut(s) 209, 238
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 1 cut(s) 33
TasI AATT 2 cut(s) 209, 238
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 1 cut(s) 31
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 1 cut(s) 153
TspRI CASTG 1 cut(s) 52
XapI RAATTY 2 cut(s) 209, 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.