RchiOBHm_Chr4g0394221

Polyubiquitin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005187
Physical Location & Seq
Reverse (-)
9827475 .. 9828652
1178 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36673

Sequence Viewer

Length: 990 bp
ATGGAAACACTGAGACACCGTAGACCCTCAAAGCGAAGATACCGAATACTCCCATCAAGTTTCTCTGGTTCATCTCAAGAACAGAGAAATTTACTGTGGAAGATCATGAAAAAGGTATCTTTAAAGTTCAAGAAATTTGTCACAGTTGAAAAATTAAAAGCCATAGTATTCCACAGCAAGAAAAGCAATTCAGGAAAGTTCCAGGAGCCTCTTATGGATAGTTTTAATCATAATGGACTTGAAGAAGACCAAATACCAGTCAGTCAAGGCATTCAGCAGGAACCCAGTTGCCAAAAACTTCACCAGAATTCAGTGAGAATGAAAATATATGTCAAAATCCCATCAAACCAGAAGAGCAGTGTATTTGAAGCAAAAGAGTACAATATCATCAATGACATCAAATCCATGATTCATTTGAAGGAGGGAATCAAATCAAATCAGTACACTTTGGTTTACGGTGGCAAAATACTACAAGATTATAGGACATTGGCATCGCTTAACATTCGAACAGAGTCAACCCTTCATATGATTTTTAATCCAAGGGATGTGATGTCAGTTTTTGTGAAAACACCAAGTGGAAAGATTGTGGAATTTGAAGTTAAGGTTTTGTACACTGTCCGTGATGTCAAGCAGATTGTTGAGAGCTTTATAGGATGTCCTGTTGTTGATTGTAGTATGGTTTATGAAGGAAATGAGCTGCAAGATTTCAAGACCTTGGCTTTCTACAACATTGAAGAAAATTCCACTCTAGAGGTGAAGCCTTCTTGGATTCAGATATTTGTCAAGACATGGAGTGGAAAAACCATTACACTTGATGTGACACGATCAAATACTGTAAGAGAGGTGAAGGAGAAGATATTTTGTAAGGTTAGAGTGCCAACAATTCGTCAGAGTATCGTATTTTCTGGAAAACGCCTTGAAGATAAATGCTCTCTTGCAAGTTACAACATTCAGAAACAGTCGACTCTCCACATGGTTATGGCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

329

Amino Acids

38.18

Weight (kDa)

9.69

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 109 - 180 1.1e-12 Ubiquitin family
Rad60-SLD PF11976 184 - 252 1.5e-06 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 186 - 252 1.6e-11 Ubiquitin family
Rad60-SLD PF11976 257 - 326 1.3e-08 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 259 - 327 3.6e-21 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 22, 962
AcsI RAATTY 5 cut(s) 88, 134, 307, 590, 739
AdeI CACNNNGTG 1 cut(s) 575
AfaI GTAC 3 cut(s) 380, 443, 611
AgsI TTSAA 9 cut(s) 130, 149, 242, 368, 418, 596, 709, 734, 920
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 2 cut(s) 645, 697
AluI AGCT 2 cut(s) 645, 697
Alw26I GTCTC 1 cut(s) 7
ApeKI GCWGC 1 cut(s) 697
ApoI RAATTY 5 cut(s) 88, 134, 307, 590, 739
AsuHPI GGTGA 3 cut(s) 293, 766, 856
AsuII TTCGAA 1 cut(s) 505
BarI GAAGNNNNNNTAC 2 cut(s) 237, 269
BbsI GAAGAC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 684
BccI CCATC 2 cut(s) 61, 349
BciT130I CCWGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 7
BfaI CTAG 1 cut(s) 749
BisI GCNGC 1 cut(s) 698
BlsI GCNGC 1 cut(s) 699
Bme1390I CCNGG 1 cut(s) 203
BmiI GGNNCC 2 cut(s) 207, 282
BmrFI CCNGG 1 cut(s) 203
BmrI ACTGGG 1 cut(s) 279
BmsI GCATC 1 cut(s) 500
BmuI ACTGGG 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 505
BpuEI CTTGAG 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 539, 714
Bse1I ACTGG 2 cut(s) 257, 285
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 539, 714
BseGI GGATG 2 cut(s) 550, 659
BseNI ACTGG 2 cut(s) 257, 285
BseXI GCAGC 1 cut(s) 684
BsmAI GTCTC 1 cut(s) 7
BsmI GAATGC 1 cut(s) 270
Bsp119I TTCGAA 1 cut(s) 505
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 2 cut(s) 102, 824
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 2 cut(s) 207, 282
BspQI GCTCTTC 1 cut(s) 347
BspT104I TTCGAA 1 cut(s) 505
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 2 cut(s) 257, 285
BssECI CCNNGG 2 cut(s) 539, 714
BssMI GATC 2 cut(s) 102, 824
BssT1I CCWWGG 2 cut(s) 539, 714
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 7 cut(s) 20, 96, 145, 458, 616, 835, 960
Bst6I CTCTTC 1 cut(s) 347
BstAUI TGTACA 1 cut(s) 609
BstBI TTCGAA 1 cut(s) 505
BstDEI CTNAG 1 cut(s) 11
BstF5I GGATG 2 cut(s) 550, 659
BstKTI GATC 2 cut(s) 105, 827
BstMAI GTCTC 1 cut(s) 7
BstMBI GATC 2 cut(s) 102, 824
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 684
BstV2I GAAGAC 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 2 cut(s) 550, 659
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 4 cut(s) 8, 318, 364, 612
CciI TCATGA 1 cut(s) 105
Csp6I GTAC 3 cut(s) 379, 442, 610
CviAII CATG 4 cut(s) 106, 406, 789, 973
CviJI RGCY 7 cut(s) 161, 208, 645, 697, 719, 760, 983
CviKI_1 RGCY 7 cut(s) 161, 208, 645, 697, 719, 760, 983
CviQI GTAC 3 cut(s) 379, 442, 610
DdeI CTNAG 1 cut(s) 11
DpnI GATC 2 cut(s) 104, 826
DpnII GATC 2 cut(s) 102, 824
DraI TTTAAA 1 cut(s) 123
DraIII CACNNNGTG 1 cut(s) 575
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 539, 714
EcoRI GAATTC 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 539, 714
ErhI CCWWGG 2 cut(s) 539, 714
FaeI CATG 4 cut(s) 109, 409, 792, 976
FatI CATG 4 cut(s) 105, 405, 788, 972
FauNDI CATATG 1 cut(s) 525
FblI GTMKAC 2 cut(s) 22, 962
Fnu4HI GCNGC 1 cut(s) 698
FokI GGATG 2 cut(s) 557, 666
Fsp4HI GCNGC 1 cut(s) 698
FspBI CTAG 1 cut(s) 749
GluI GCNGC 1 cut(s) 698
Hin1II CATG 4 cut(s) 109, 409, 792, 976
HincII GTYRAC 2 cut(s) 516, 963
HindII GTYRAC 2 cut(s) 516, 963
HinfI GANTC 5 cut(s) 409, 426, 512, 769, 964
HphI GGTGA 3 cut(s) 293, 766, 856
Hpy166II GTNNAC 6 cut(s) 23, 444, 454, 516, 612, 963
Hpy188I TCNGA 3 cut(s) 774, 891, 954
Hpy188III TCNNGA 8 cut(s) 77, 106, 130, 192, 709, 749, 784, 906
Hpy8I GTNNAC 6 cut(s) 23, 444, 454, 516, 612, 963
HpyAV CCTTC 5 cut(s) 412, 530, 680, 771, 841
HpyCH4III ACNGT 7 cut(s) 20, 96, 145, 458, 616, 835, 960
HpyCH4V TGCA 2 cut(s) 700, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 1 cut(s) 11
Hsp92II CATG 4 cut(s) 109, 409, 792, 976
Kzo9I GATC 2 cut(s) 102, 824
LguI GCTCTTC 1 cut(s) 347
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 1 cut(s) 684
LweI GCATC 1 cut(s) 500
MaeI CTAG 1 cut(s) 749
MaeIII GTNAC 3 cut(s) 139, 817, 941
MalI GATC 2 cut(s) 104, 826
MboI GATC 2 cut(s) 102, 824
MboII GAAGA 8 cut(s) 48, 112, 254, 257, 364, 746, 865, 932
MluCI AATT 8 cut(s) 88, 134, 152, 187, 307, 590, 739, 882
MlyI GAGTC 2 cut(s) 521, 958
MnlI CCTC 5 cut(s) 37, 219, 415, 745, 835
MseI TTAA 6 cut(s) 122, 155, 225, 498, 534, 600
MslI CAYNNNNRTG 1 cut(s) 977
MspR9I CCNGG 1 cut(s) 203
Mva1269I GAATGC 1 cut(s) 270
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 1 cut(s) 183
NdeI CATATG 1 cut(s) 525
NdeII GATC 2 cut(s) 102, 824
NlaIII CATG 4 cut(s) 109, 409, 792, 976
NlaIV GGNNCC 2 cut(s) 207, 282
NmuCI GTSAC 2 cut(s) 139, 817
NspV TTCGAA 1 cut(s) 505
PagI TCATGA 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 347
PctI GAATGC 1 cut(s) 270
PfeI GAWTC 3 cut(s) 409, 426, 769
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 1 cut(s) 699
PleI GAGTC 2 cut(s) 520, 958
PpsI GAGTC 2 cut(s) 520, 958
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 207, 282
RsaI GTAC 3 cut(s) 380, 443, 611
RsaNI GTAC 3 cut(s) 379, 442, 610
RseI CAYNNNNRTG 1 cut(s) 977
SalI GTCGAC 1 cut(s) 961
SapI GCTCTTC 1 cut(s) 347
SaqAI TTAA 6 cut(s) 122, 155, 225, 498, 534, 600
SatI GCNGC 1 cut(s) 698
Sau3AI GATC 2 cut(s) 102, 824
SchI GAGTC 2 cut(s) 521, 958
ScrFI CCNGG 1 cut(s) 203
SetI ASST 8 cut(s) 117, 606, 647, 699, 716, 756, 846, 870
SfaNI GCATC 1 cut(s) 500
SfuI TTCGAA 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 977
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 8 cut(s) 88, 134, 152, 187, 307, 590, 739, 882
SspMI CTAG 1 cut(s) 749
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 2 cut(s) 539, 714
TaaI ACNGT 7 cut(s) 20, 96, 145, 458, 616, 835, 960
TaqI TCGA 2 cut(s) 505, 962
TasI AATT 8 cut(s) 88, 134, 152, 187, 307, 590, 739, 882
TatI WGTACW 3 cut(s) 378, 441, 609
TfiI GAWTC 3 cut(s) 409, 426, 769
Tru1I TTAA 6 cut(s) 122, 155, 225, 498, 534, 600
Tru9I TTAA 6 cut(s) 122, 155, 225, 498, 534, 600
TscAI CASTG 4 cut(s) 15, 318, 364, 619
TseFI GTSAC 2 cut(s) 139, 817
TseI GCWGC 1 cut(s) 697
Tsp45I GTSAC 2 cut(s) 139, 817
TspDTI ATGAA 6 cut(s) 60, 122, 335, 401, 512, 699
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 4 cut(s) 15, 318, 364, 619
XapI RAATTY 5 cut(s) 88, 134, 307, 590, 739
XbaI TCTAGA 1 cut(s) 748
XmiI GTMKAC 2 cut(s) 22, 962
XspI CTAG 1 cut(s) 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.