Rroxscaffold_5G00339280

Polyubiquitin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005187
Physical Location & Seq
Reverse (-)
7630887 .. 7632388
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00339280.1

Sequence Viewer

Length: 852 bp
ATGGAAACACTGAGACACCGTAGACCCTCAAAGCGAAGATACCGAATACTCCCATCAAGTTTCTCTGGTTCATCTCAAGAACAGTTTTATCATAATGGACTTGAAGAAGACCAAATACCAGTTAGTCAAGGCATTCAGCAGGAACCCAGTTGCCAAAAACTTCATCAGAATTCAGTGAGAATGAAAATATATGTCAAAATCCCATCAAATCAGAAGAGCAGTGTATTTGAAGCAAAAGAATACAATATCATCAATGACATCAAATCCATGATTCATTTGAAGGAGGGAATCCAATCAAATCAGTACACTTTGGTTTACGGTGGCAAAATACTTCAAGATTATAGGACATTGGCCTCGCTTAACATTCGAACAGAGTCAACCCTTCATATGATTTTTAACCCAAAAGATGTGATGTCAGTTTTTGTGAAAACACCAAGTGGAAAGATTGTGGAATTTGAAGTTAAGGTCTTGTACACTGTCCGTGATGTCAAGCAGATTGTTGAGAGCTTTATAGGATGTTCTGTTGTTGATTGTAGCATGGTTTATGAAGGAAATGAGCTGCAAGATTTCAAGACCTTGGCTTTCTACAACATTGAAGAAAATTCCACTCTAGAGGTGAAGCCTTCTTGGATTCAGATATTTGTCAAGACATGGAGTGGAAAAACCATTACACTTGATGTGACACGATCAAATACTGTAAGAGAGGTGAAGGAGAAGATATTTTGTAAGGTTAGAGTGCCAACAATTCGTCAGAGTATCGTATTTTCTGGAAAACGCCTTGAAGATAAATGCTCTCTTGCAAGTTACAACATTCAGAAACAGTCGACTCTCCACATGGTTATGGCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

283

Amino Acids

32.77

Weight (kDa)

9.29

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 63 - 134 6.5e-13 Ubiquitin family
Rad60-SLD PF11976 138 - 206 1.7e-06 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 140 - 206 7.8e-11 Ubiquitin family
Rad60-SLD PF11976 211 - 280 9.8e-09 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 213 - 281 2.8e-21 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 22, 824
AcsI RAATTY 3 cut(s) 169, 452, 601
AdeI CACNNNGTG 1 cut(s) 437
AfaI GTAC 2 cut(s) 305, 473
AgsI TTSAA 8 cut(s) 104, 230, 280, 335, 458, 571, 596, 782
AluBI AGCT 2 cut(s) 507, 559
AluI AGCT 2 cut(s) 507, 559
Alw26I GTCTC 1 cut(s) 7
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 1 cut(s) 559
ApoI RAATTY 3 cut(s) 169, 452, 601
AsuHPI GGTGA 2 cut(s) 628, 718
AsuII TTCGAA 1 cut(s) 367
BarI GAAGNNNNNNTAC 2 cut(s) 99, 131
BbsI GAAGAC 1 cut(s) 114
BbvI GCAGC 1 cut(s) 546
BccI CCATC 2 cut(s) 61, 211
BcoDI GTCTC 1 cut(s) 7
BfaI CTAG 1 cut(s) 611
BisI GCNGC 1 cut(s) 560
BlsI GCNGC 1 cut(s) 561
BmiI GGNNCC 1 cut(s) 144
BmrI ACTGGG 1 cut(s) 141
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 114
Bpu14I TTCGAA 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 576
Bse1I ACTGG 2 cut(s) 119, 147
BseDI CCNNGG 1 cut(s) 576
BseGI GGATG 1 cut(s) 521
BseNI ACTGG 2 cut(s) 119, 147
BseXI GCAGC 1 cut(s) 546
BshFI GGCC 1 cut(s) 353
BsmAI GTCTC 1 cut(s) 7
BsmI GAATGC 1 cut(s) 132
BsnI GGCC 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 367
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 1 cut(s) 686
BspANI GGCC 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 144
BspQI GCTCTTC 1 cut(s) 209
BspT104I TTCGAA 1 cut(s) 367
BsrGI TGTACA 1 cut(s) 471
BsrI ACTGG 2 cut(s) 119, 147
BssECI CCNNGG 1 cut(s) 576
BssMI GATC 1 cut(s) 686
BssT1I CCWWGG 1 cut(s) 576
Bst4CI ACNGT 6 cut(s) 20, 84, 320, 478, 697, 822
Bst6I CTCTTC 1 cut(s) 209
BstAUI TGTACA 1 cut(s) 471
BstBI TTCGAA 1 cut(s) 367
BstDEI CTNAG 1 cut(s) 11
BstF5I GGATG 1 cut(s) 521
BstKTI GATC 1 cut(s) 689
BstMAI GTCTC 1 cut(s) 7
BstMBI GATC 1 cut(s) 686
BstV1I GCAGC 1 cut(s) 546
BstV2I GAAGAC 1 cut(s) 114
BsuRI GGCC 1 cut(s) 353
BtsCI GGATG 1 cut(s) 521
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 4 cut(s) 8, 180, 226, 474
Csp6I GTAC 2 cut(s) 304, 472
CviAII CATG 4 cut(s) 268, 538, 651, 835
CviJI RGCY 6 cut(s) 353, 507, 559, 581, 622, 845
CviKI_1 RGCY 6 cut(s) 353, 507, 559, 581, 622, 845
CviQI GTAC 2 cut(s) 304, 472
DdeI CTNAG 1 cut(s) 11
DpnI GATC 1 cut(s) 688
DpnII GATC 1 cut(s) 686
DraIII CACNNNGTG 1 cut(s) 437
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 576
EcoRI GAATTC 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 576
FaeI CATG 4 cut(s) 271, 541, 654, 838
FatI CATG 4 cut(s) 267, 537, 650, 834
FauNDI CATATG 1 cut(s) 387
FblI GTMKAC 2 cut(s) 22, 824
Fnu4HI GCNGC 1 cut(s) 560
FokI GGATG 1 cut(s) 528
Fsp4HI GCNGC 1 cut(s) 560
FspBI CTAG 1 cut(s) 611
GluI GCNGC 1 cut(s) 560
HaeIII GGCC 1 cut(s) 353
Hin1II CATG 4 cut(s) 271, 541, 654, 838
HincII GTYRAC 2 cut(s) 378, 825
HindII GTYRAC 2 cut(s) 378, 825
HinfI GANTC 5 cut(s) 271, 288, 374, 631, 826
HphI GGTGA 2 cut(s) 628, 718
Hpy166II GTNNAC 6 cut(s) 23, 306, 316, 378, 474, 825
Hpy188I TCNGA 5 cut(s) 168, 213, 636, 753, 816
Hpy188III TCNNGA 6 cut(s) 77, 335, 571, 611, 646, 768
Hpy8I GTNNAC 6 cut(s) 23, 306, 316, 378, 474, 825
HpyAV CCTTC 5 cut(s) 274, 392, 542, 633, 703
HpyCH4III ACNGT 6 cut(s) 20, 84, 320, 478, 697, 822
HpyCH4V TGCA 2 cut(s) 562, 800
HpyF3I CTNAG 1 cut(s) 11
Hsp92II CATG 4 cut(s) 271, 541, 654, 838
Kzo9I GATC 1 cut(s) 686
LguI GCTCTTC 1 cut(s) 209
LpnPI CCDG 5 cut(s) 51, 125, 132, 160, 753
Lsp1109I GCAGC 1 cut(s) 546
MaeI CTAG 1 cut(s) 611
MaeIII GTNAC 2 cut(s) 679, 803
MalI GATC 1 cut(s) 688
MboI GATC 1 cut(s) 686
MboII GAAGA 7 cut(s) 48, 116, 119, 226, 608, 727, 794
MluCI AATT 4 cut(s) 169, 452, 601, 744
MlyI GAGTC 2 cut(s) 383, 820
MnlI CCTC 5 cut(s) 37, 277, 364, 607, 697
MseI TTAA 3 cut(s) 360, 396, 462
MslI CAYNNNNRTG 1 cut(s) 839
Mva1269I GAATGC 1 cut(s) 132
NdeI CATATG 1 cut(s) 387
NdeII GATC 1 cut(s) 686
NlaIII CATG 4 cut(s) 271, 541, 654, 838
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 679
NspV TTCGAA 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 209
PctI GAATGC 1 cut(s) 132
PfeI GAWTC 3 cut(s) 271, 288, 631
PkrI GCNGC 1 cut(s) 561
PleI GAGTC 2 cut(s) 382, 820
PpsI GAGTC 2 cut(s) 382, 820
PspN4I GGNNCC 1 cut(s) 144
RsaI GTAC 2 cut(s) 305, 473
RsaNI GTAC 2 cut(s) 304, 472
RseI CAYNNNNRTG 1 cut(s) 839
SalI GTCGAC 1 cut(s) 823
SapI GCTCTTC 1 cut(s) 209
SaqAI TTAA 3 cut(s) 360, 396, 462
SatI GCNGC 1 cut(s) 560
Sau3AI GATC 1 cut(s) 686
SchI GAGTC 2 cut(s) 383, 820
SetI ASST 7 cut(s) 468, 509, 561, 578, 618, 708, 732
SfuI TTCGAA 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 839
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 4 cut(s) 169, 452, 601, 744
SspMI CTAG 1 cut(s) 611
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 6 cut(s) 20, 84, 320, 478, 697, 822
TaqI TCGA 2 cut(s) 367, 824
TasI AATT 4 cut(s) 169, 452, 601, 744
TatI WGTACW 2 cut(s) 303, 471
TfiI GAWTC 3 cut(s) 271, 288, 631
Tru1I TTAA 3 cut(s) 360, 396, 462
Tru9I TTAA 3 cut(s) 360, 396, 462
TscAI CASTG 4 cut(s) 15, 180, 226, 481
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 1 cut(s) 559
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 6 cut(s) 60, 152, 197, 263, 374, 561
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 4 cut(s) 15, 180, 226, 481
XapI RAATTY 3 cut(s) 169, 452, 601
XbaI TCTAGA 1 cut(s) 610
XmiI GTMKAC 2 cut(s) 22, 824
XspI CTAG 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.