MD16G1260600.v1.1

tetratricopeptide repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003372
Physical Location & Seq
Forward (+)
32127103 .. 32138508
11406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1260600.v1.1.491

Sequence Viewer

Length: 762 bp
ATGCTGATAAAGGAAGAAGAAGGAGAGAATGAATGCCATAGCGATATGAATCCTCCAAATCCCTCCTCCTCCTCTTCCACCGCCACCAACAGAGATGCCTCCGATGGCTTTGAAACTGCCAGCGACGGCGAACTCAACGACGACGAAATCCCTCAACAACTCCATCTCCAAGACGAACAACAAAAAACTCCCTCTCAGAACGACGACTACGTTGAATCGAAGCAGAAAGCGTTTGAAGAAGCAAGTGATGTGAAAATTGAAGGAAATAGACTATTTGGCAGTGGGCAATACCAGGAGGCACTAACACAGTATGAGCTTGCTTTACACCTTGCACCTGATATGCCAGCGTCTGTCGAATTACGTTCCATTTGTCATTTAAACAGTGCAGTATGCTTCTCGAAATTGGAAAAGTACGAGGACGCAATCAAGGAATGCACAAAAGCGCTTGAACTCAATCCTTCATATATGAAAGCTCTGCTTAGAAGAGCAGAAACTCATGAAAAGCTCGAGCATTTTGAAGAGGCTATTGTTGATATGAAAAGTGTCTTAGAACTTGATCCTTCGAATGACCAAGCCAAAATGGCCATTCGCCGTTTGGGGCCGCTAGCTGAAGAAAAGAGAGAAAAGATGAAAGAGGAAATGATAGGGAAGCTGAAAGAAATGGGCAACTCGCTTTTGGGCCGTTTTGGAATGAGCGTCGACAACTTTAAATCTGTCAAAGATCCAAACACTGGCGCGTATTCTCTTTCATTCCAACGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_1 TPR_2 TPR_8

Protein Analysis

254

Amino Acids

28.49

Weight (kDa)

4.76

Isoelectric Point (pI)

50.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_1 PF00515 124 - 155 3e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 731
AccI GTMKAC 1 cut(s) 699
AccII CGCG 1 cut(s) 737
AciI CCGC 2 cut(s) 81, 602
AclWI GGATC 2 cut(s) 551, 716
AcoI YGGCCR 1 cut(s) 582
AcuI CTGAAG 1 cut(s) 630
AfaI GTAC 1 cut(s) 413
AfeI AGCGCT 1 cut(s) 444
AfiI CCNNNNNNNGG 1 cut(s) 731
AgsI TTSAA 6 cut(s) 113, 215, 236, 260, 449, 518
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 5 cut(s) 316, 473, 505, 608, 652
AluI AGCT 5 cut(s) 316, 473, 505, 608, 652
AlwI GGATC 2 cut(s) 551, 716
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 506
Aor51HI AGCGCT 1 cut(s) 444
AoxI GGCC 3 cut(s) 582, 599, 679
AspLEI GCGC 2 cut(s) 445, 737
AspS9I GGNCC 2 cut(s) 599, 679
AsuII TTCGAA 1 cut(s) 563
AsuNHI GCTAGC 1 cut(s) 604
AvaI CYCGRG 1 cut(s) 506
BalI TGGCCA 1 cut(s) 584
BccI CCATC 2 cut(s) 98, 171
BceAI ACGGC 3 cut(s) 142, 576, 666
BciT130I CCWGG 1 cut(s) 293
BfaI CTAG 2 cut(s) 605, 760
BfoI RGCGCY 1 cut(s) 446
BglI GCCNNNNNGGC 1 cut(s) 581
BisI GCNGC 1 cut(s) 602
BlsI GCNGC 1 cut(s) 603
Bme1390I CCNGG 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 2 cut(s) 599, 679
BmiI GGNNCC 1 cut(s) 600
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 85
BmtI GCTAGC 1 cut(s) 608
Bpu14I TTCGAA 1 cut(s) 563
BsaBI GATNNNNATC 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 731
Bse1I ACTGG 1 cut(s) 736
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 1 cut(s) 293
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 731
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 1 cut(s) 736
BseRI GAGGAG 3 cut(s) 55, 58, 61
BsgI GTGCAG 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 737
BshFI GGCC 3 cut(s) 584, 601, 681
BsiHKCI CYCGRG 1 cut(s) 506
BslI CCNNNNNNNGG 1 cut(s) 731
BsmI GAATGC 2 cut(s) 38, 437
BsnI GGCC 3 cut(s) 584, 601, 681
BsoBI CYCGRG 1 cut(s) 506
Bsp119I TTCGAA 1 cut(s) 563
Bsp143I GATC 2 cut(s) 556, 721
BspACI CCGC 2 cut(s) 81, 602
BspANI GGCC 3 cut(s) 584, 601, 681
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 1 cut(s) 737
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 1 cut(s) 600
BspOI GCTAGC 1 cut(s) 608
BspPI GGATC 2 cut(s) 551, 716
BspQI GCTCTTC 1 cut(s) 478
BspT104I TTCGAA 1 cut(s) 563
BsrI ACTGG 1 cut(s) 736
BssMI GATC 2 cut(s) 556, 721
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 309, 383
Bst6I CTCTTC 3 cut(s) 79, 478, 513
BstBI TTCGAA 1 cut(s) 563
BstC8I GCNNGC 4 cut(s) 121, 318, 345, 606
BstDEI CTNAG 3 cut(s) 195, 479, 547
BstFNI CGCG 1 cut(s) 737
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 2 cut(s) 445, 737
BstKTI GATC 2 cut(s) 559, 724
BstMBI GATC 2 cut(s) 556, 721
BstMWI GCNNNNNNNGC 1 cut(s) 581
BstNI CCWGG 1 cut(s) 293
BstSCI CCNGG 1 cut(s) 291
BstUI CGCG 1 cut(s) 737
BstX2I RGATCY 1 cut(s) 721
BstYI RGATCY 1 cut(s) 721
BsuRI GGCC 3 cut(s) 584, 601, 681
BtsI GCAGTG 1 cut(s) 286
BtsIMutI CAGTG 3 cut(s) 286, 388, 729
Cac8I GCNNGC 4 cut(s) 121, 318, 345, 606
CaiI CAGNNNCTG 1 cut(s) 350
CciI TCATGA 1 cut(s) 496
CfoI GCGC 2 cut(s) 445, 737
Cfr13I GGNCC 2 cut(s) 599, 679
CseI GACGC 3 cut(s) 336, 428, 685
Csp6I GTAC 1 cut(s) 412
CviAII CATG 1 cut(s) 497
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 3 cut(s) 195, 479, 547
DpnI GATC 2 cut(s) 558, 723
DpnII GATC 2 cut(s) 556, 721
DraI TTTAAA 2 cut(s) 378, 709
EaeI YGGCCR 1 cut(s) 582
Eam1104I CTCTTC 3 cut(s) 79, 478, 513
EarI CTCTTC 3 cut(s) 79, 478, 513
Eco47III AGCGCT 1 cut(s) 444
Eco57I CTGAAG 1 cut(s) 630
Eco88I CYCGRG 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 291
FaeI CATG 1 cut(s) 500
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FatI CATG 1 cut(s) 496
FblI GTMKAC 1 cut(s) 699
Fnu4HI GCNGC 1 cut(s) 602
Fsp4HI GCNGC 1 cut(s) 602
FspBI CTAG 2 cut(s) 605, 760
GlaI GCGC 2 cut(s) 444, 736
GluI GCNGC 1 cut(s) 602
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 3 cut(s) 584, 601, 681
HgaI GACGC 3 cut(s) 336, 428, 685
HhaI GCGC 2 cut(s) 445, 737
Hin1II CATG 1 cut(s) 500
Hin6I GCGC 2 cut(s) 443, 735
HinP1I GCGC 2 cut(s) 443, 735
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HinfI GANTC 2 cut(s) 49, 215
Hpy166II GTNNAC 1 cut(s) 700
Hpy188I TCNGA 2 cut(s) 103, 198
Hpy188III TCNNGA 2 cut(s) 397, 497
Hpy8I GTNNAC 1 cut(s) 700
Hpy99I CGWCG 5 cut(s) 128, 143, 146, 206, 701
HpyAV CCTTC 4 cut(s) 14, 254, 468, 570
HpyCH4III ACNGT 2 cut(s) 309, 383
HpyCH4IV ACGT 2 cut(s) 210, 361
HpyCH4V TGCA 3 cut(s) 332, 386, 435
HpyF10VI GCNNNNNNNGC 1 cut(s) 581
HpyF3I CTNAG 3 cut(s) 195, 479, 547
HpySE526I ACGT 2 cut(s) 210, 361
Hsp92II CATG 1 cut(s) 500
HspAI GCGC 2 cut(s) 443, 735
Kzo9I GATC 2 cut(s) 556, 721
LguI GCTCTTC 1 cut(s) 478
LpnPI CCDG 6 cut(s) 133, 278, 305, 348, 357, 717
LweI GCATC 1 cut(s) 85
MaeI CTAG 2 cut(s) 605, 760
MaeII ACGT 2 cut(s) 210, 361
MalI GATC 2 cut(s) 558, 723
MboI GATC 2 cut(s) 556, 721
MboII GAAGA 7 cut(s) 26, 29, 66, 248, 495, 530, 623
MflI RGATCY 1 cut(s) 721
MlsI TGGCCA 1 cut(s) 584
MluCI AATT 3 cut(s) 255, 356, 401
MluNI TGGCCA 1 cut(s) 584
Mox20I TGGCCA 1 cut(s) 584
MscI TGGCCA 1 cut(s) 584
MseI TTAA 2 cut(s) 377, 708
Msp20I TGGCCA 1 cut(s) 584
MspR9I CCNGG 1 cut(s) 293
Mva1269I GAATGC 2 cut(s) 38, 437
MvaI CCWGG 1 cut(s) 293
MvnI CGCG 1 cut(s) 737
MwoI GCNNNNNNNGC 1 cut(s) 581
NdeII GATC 2 cut(s) 556, 721
NheI GCTAGC 1 cut(s) 604
NlaIII CATG 1 cut(s) 500
NlaIV GGNNCC 1 cut(s) 600
NspV TTCGAA 1 cut(s) 563
PaeR7I CTCGAG 1 cut(s) 506
PagI TCATGA 1 cut(s) 496
PciSI GCTCTTC 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 2 cut(s) 38, 437
PfeI GAWTC 2 cut(s) 49, 215
PflMI CCANNNNNTGG 1 cut(s) 731
PkrI GCNGC 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 1 cut(s) 600
PspPI GGNCC 2 cut(s) 599, 679
PspXI VCTCGAGB 1 cut(s) 506
PsrI GAACNNNNNNTAC 2 cut(s) 191, 223
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 721
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
SalI GTCGAC 1 cut(s) 698
SapI GCTCTTC 1 cut(s) 478
SaqAI TTAA 2 cut(s) 377, 708
SatI GCNGC 1 cut(s) 602
Sau3AI GATC 2 cut(s) 556, 721
Sau96I GGNCC 2 cut(s) 599, 679
ScrFI CCNGG 1 cut(s) 293
SetI ASST 9 cut(s) 213, 318, 330, 337, 364, 475, 507, 610, 654
SfaNI GCATC 1 cut(s) 85
Sfr274I CTCGAG 1 cut(s) 506
SfuI TTCGAA 1 cut(s) 563
SlaI CTCGAG 1 cut(s) 506
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 3 cut(s) 255, 356, 401
SsiI CCGC 2 cut(s) 81, 602
SspMI CTAG 2 cut(s) 605, 760
StyD4I CCNGG 1 cut(s) 291
TaaI ACNGT 2 cut(s) 309, 383
TaiI ACGT 2 cut(s) 213, 364
TaqI TCGA 6 cut(s) 218, 354, 398, 507, 563, 699
TasI AATT 3 cut(s) 255, 356, 401
TauI GCSGC 1 cut(s) 604
TfiI GAWTC 2 cut(s) 49, 215
Tru1I TTAA 2 cut(s) 377, 708
Tru9I TTAA 2 cut(s) 377, 708
TscAI CASTG 3 cut(s) 286, 388, 736
TspDTI ATGAA 8 cut(s) 45, 62, 450, 482, 513, 551, 644, 738
TspRI CASTG 3 cut(s) 286, 388, 736
Van91I CCANNNNNTGG 1 cut(s) 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XhoI CTCGAG 1 cut(s) 506
XmiI GTMKAC 1 cut(s) 699
XspI CTAG 2 cut(s) 605, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.