Belongs to the STXBP unc-18 SEC1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1714 |
| AccBSI | CCGCTC | 1 cut(s) | 569 |
| AccII | CGCG | 2 cut(s) | 176, 1549 |
| AciI | CCGC | 5 cut(s) | 36, 567, 616, 1106, 1300 |
| AclWI | GGATC | 6 cut(s) | 704, 929, 936, 1224, 1438, 1916 |
| AcoI | YGGCCR | 2 cut(s) | 564, 1730 |
| AcsI | RAATTY | 6 cut(s) | 49, 445, 602, 1256, 1337, 1738 |
| AcuI | CTGAAG | 2 cut(s) | 343, 1493 |
| AdeI | CACNNNGTG | 1 cut(s) | 1664 |
| AfaI | GTAC | 1 cut(s) | 135 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1526, 1749 |
| AflIII | ACRYGT | 2 cut(s) | 174, 315 |
| AjnI | CCWGG | 2 cut(s) | 125, 471 |
| AleI | CACNNNNGTG | 1 cut(s) | 1044 |
| Alw21I | GWGCWC | 2 cut(s) | 331, 1971 |
| Alw26I | GTCTC | 2 cut(s) | 713, 740 |
| AlwI | GGATC | 6 cut(s) | 704, 929, 936, 1224, 1438, 1916 |
| Ama87I | CYCGRG | 1 cut(s) | 1059 |
| AoxI | GGCC | 2 cut(s) | 564, 1730 |
| ApeKI | GCWGC | 7 cut(s) | 428, 626, 689, 1028, 1319, 1540, 1667 |
| ApoI | RAATTY | 6 cut(s) | 49, 445, 602, 1256, 1337, 1738 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 90, 1896 |
| AspS9I | GGNCC | 2 cut(s) | 96, 896 |
| AsuC2I | CCSGG | 1 cut(s) | 1178 |
| AsuHPI | GGTGA | 4 cut(s) | 475, 1298, 1358, 1607 |
| AvaI | CYCGRG | 1 cut(s) | 1059 |
| AvaII | GGWCC | 2 cut(s) | 96, 896 |
| BaeGI | GKGCMC | 2 cut(s) | 1717, 1729 |
| BanI | GGYRCC | 1 cut(s) | 1714 |
| BanII | GRGCYC | 1 cut(s) | 1971 |
| BauI | CACGAG | 1 cut(s) | 570 |
| BbsI | GAAGAC | 2 cut(s) | 228, 244 |
| Bbv12I | GWGCWC | 2 cut(s) | 331, 1971 |
| BbvCI | CCTCAGC | 1 cut(s) | 1883 |
| BbvI | GCAGC | 7 cut(s) | 415, 613, 676, 1015, 1331, 1552, 1679 |
| BccI | CCATC | 6 cut(s) | 556, 848, 1084, 1568, 1742, 1795 |
| BceAI | ACGGC | 3 cut(s) | 51, 1745, 1974 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1063, 1097 |
| BciT130I | CCWGG | 2 cut(s) | 127, 473 |
| BclI | TGATCA | 2 cut(s) | 297, 1251 |
| BcnI | CCSGG | 1 cut(s) | 1178 |
| BcoDI | GTCTC | 2 cut(s) | 713, 740 |
| BfaI | CTAG | 4 cut(s) | 978, 1785, 1842, 1901 |
| BfmI | CTRYAG | 2 cut(s) | 462, 783 |
| BglII | AGATCT | 1 cut(s) | 779 |
| Bme1390I | CCNGG | 3 cut(s) | 127, 473, 1178 |
| Bme18I | GGWCC | 2 cut(s) | 96, 896 |
| BmeT110I | CYCGRG | 1 cut(s) | 1059 |
| BmgT120I | GGNCC | 2 cut(s) | 96, 896 |
| BmiI | GGNNCC | 4 cut(s) | 879, 1716, 1837, 1906 |
| BmrFI | CCNGG | 3 cut(s) | 127, 473, 1178 |
| BmsI | GCATC | 7 cut(s) | 250, 394, 631, 814, 964, 1045, 1222 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1635 |
| BpiI | GAAGAC | 2 cut(s) | 228, 244 |
| BpmI | CTGGAG | 3 cut(s) | 1229, 1668, 1970 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1586, 1853, 1883 |
| BpuEI | CTTGAG | 1 cut(s) | 968 |
| BpuMI | CCSGG | 1 cut(s) | 1178 |
| BsaBI | GATNNNNATC | 1 cut(s) | 933 |
| BsaJI | CCNNGG | 2 cut(s) | 255, 1042 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1526, 1749 |
| Bse1I | ACTGG | 3 cut(s) | 895, 1288, 1685 |
| Bse3DI | GCAATG | 2 cut(s) | 245, 1100 |
| Bse8I | GATNNNNATC | 1 cut(s) | 933 |
| BseBI | CCWGG | 2 cut(s) | 127, 473 |
| BseDI | CCNNGG | 2 cut(s) | 255, 1042 |
| BseGI | GGATG | 5 cut(s) | 265, 409, 526, 859, 1984 |
| BseJI | GATNNNNATC | 1 cut(s) | 933 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1526, 1749 |
| BseMI | GCAATG | 2 cut(s) | 245, 1100 |
| BseMII | CTCAG | 4 cut(s) | 735, 894, 1323, 1874 |
| BseNI | ACTGG | 3 cut(s) | 895, 1288, 1685 |
| BseRI | GAGGAG | 3 cut(s) | 539, 1897, 1900 |
| BseSI | GKGCMC | 2 cut(s) | 1717, 1729 |
| BseX3I | CGGCCG | 1 cut(s) | 1730 |
| BseXI | GCAGC | 7 cut(s) | 415, 613, 676, 1015, 1331, 1552, 1679 |
| BseYI | CCCAGC | 1 cut(s) | 692 |
| BsgI | GTGCAG | 3 cut(s) | 1014, 1686, 1911 |
| Bsh1236I | CGCG | 2 cut(s) | 176, 1549 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1733 |
| BshFI | GGCC | 2 cut(s) | 566, 1732 |
| BshNI | GGYRCC | 1 cut(s) | 1714 |
| BsiEI | CGRYCG | 1 cut(s) | 1733 |
| BsiHKAI | GWGCWC | 2 cut(s) | 331, 1971 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1059 |
| BsiSI | CCGG | 1 cut(s) | 1177 |
| BslFI | GGGAC | 4 cut(s) | 125, 1091, 1666, 1818 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1526, 1749 |
| BsmAI | GTCTC | 2 cut(s) | 713, 740 |
| BsmFI | GGGAC | 4 cut(s) | 125, 1091, 1666, 1818 |
| BsmI | GAATGC | 1 cut(s) | 1405 |
| BsnI | GGCC | 2 cut(s) | 566, 1732 |
| BsoBI | CYCGRG | 1 cut(s) | 1059 |
| Bsp1286I | GDGCHC | 4 cut(s) | 331, 1717, 1729, 1971 |
| Bsp19I | CCATGG | 2 cut(s) | 255, 1042 |
| BspACI | CCGC | 5 cut(s) | 36, 567, 616, 1106, 1300 |
| BspANI | GGCC | 2 cut(s) | 566, 1732 |
| BspCNI | CTCAG | 4 cut(s) | 736, 895, 1324, 1875 |
| BspFNI | CGCG | 2 cut(s) | 176, 1549 |
| BspHI | TCATGA | 2 cut(s) | 711, 931 |
| BspLI | GGNNCC | 4 cut(s) | 879, 1716, 1837, 1906 |
| BspPI | GGATC | 6 cut(s) | 704, 929, 936, 1224, 1438, 1916 |
| BspQI | GCTCTTC | 1 cut(s) | 319 |
| BspT107I | GGYRCC | 1 cut(s) | 1714 |
| BsrBI | CCGCTC | 1 cut(s) | 569 |
| BsrDI | GCAATG | 2 cut(s) | 245, 1100 |
| BsrI | ACTGG | 3 cut(s) | 895, 1288, 1685 |
| BssECI | CCNNGG | 2 cut(s) | 255, 1042 |
| BssSI | CACGAG | 1 cut(s) | 570 |
| BssT1I | CCWWGG | 2 cut(s) | 255, 1042 |
| Bst2BI | CACGAG | 1 cut(s) | 570 |
| Bst2UI | CCWGG | 2 cut(s) | 127, 473 |
| Bst4CI | ACNGT | 5 cut(s) | 157, 412, 583, 1115, 1602 |
| Bst6I | CTCTTC | 1 cut(s) | 319 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1055 |
| BstC8I | GCNNGC | 3 cut(s) | 178, 687, 1779 |
| BstDEI | CTNAG | 8 cut(s) | 744, 903, 1332, 1586, 1605, 1853, 1878, 1883 |
| BstDSI | CCRYGG | 2 cut(s) | 255, 1042 |
| BstF5I | GGATG | 5 cut(s) | 265, 409, 526, 859, 1984 |
| BstFNI | CGCG | 2 cut(s) | 176, 1549 |
| BstMAI | GTCTC | 2 cut(s) | 713, 740 |
| BstMCI | CGRYCG | 1 cut(s) | 1733 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 241, 575, 968, 1055, 1546 |
| BstNI | CCWGG | 2 cut(s) | 127, 473 |
| BstNSI | RCATGY | 4 cut(s) | 319, 552, 759, 1781 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1635 |
| BstSCI | CCNGG | 3 cut(s) | 125, 471, 1176 |
| BstSFI | CTRYAG | 2 cut(s) | 462, 783 |
| BstSLI | GKGCMC | 2 cut(s) | 1717, 1729 |
| BstUI | CGCG | 2 cut(s) | 176, 1549 |
| BstV1I | GCAGC | 7 cut(s) | 415, 613, 676, 1015, 1331, 1552, 1679 |
| BstV2I | GAAGAC | 2 cut(s) | 228, 244 |
| BstX2I | RGATCY | 3 cut(s) | 696, 779, 1216 |
| BstYI | RGATCY | 3 cut(s) | 696, 779, 1216 |
| BstZI | CGGCCG | 1 cut(s) | 1730 |
| BsuRI | GGCC | 2 cut(s) | 566, 1732 |
| BtgI | CCRYGG | 2 cut(s) | 255, 1042 |
| BtsCI | GGATG | 5 cut(s) | 265, 409, 526, 859, 1984 |
| BtsIMutI | CAGTG | 1 cut(s) | 1120 |
| Cac8I | GCNNGC | 3 cut(s) | 178, 687, 1779 |
| CciI | TCATGA | 2 cut(s) | 711, 931 |
| Cfr13I | GGNCC | 2 cut(s) | 96, 896 |
| Csp6I | GTAC | 1 cut(s) | 134 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 568, 603 |
| CviQI | GTAC | 1 cut(s) | 134 |
| DdeI | CTNAG | 8 cut(s) | 744, 903, 1332, 1586, 1605, 1853, 1878, 1883 |
| DraI | TTTAAA | 1 cut(s) | 1626 |
| DraIII | CACNNNGTG | 1 cut(s) | 1664 |
| EaeI | YGGCCR | 2 cut(s) | 564, 1730 |
| EagI | CGGCCG | 1 cut(s) | 1730 |
| Eam1104I | CTCTTC | 1 cut(s) | 319 |
| EarI | CTCTTC | 1 cut(s) | 319 |
| Ecl136II | GAGCTC | 1 cut(s) | 1969 |
| EclXI | CGGCCG | 1 cut(s) | 1730 |
| Eco130I | CCWWGG | 2 cut(s) | 255, 1042 |
| Eco24I | GRGCYC | 1 cut(s) | 1971 |
| Eco47I | GGWCC | 2 cut(s) | 96, 896 |
| Eco52I | CGGCCG | 1 cut(s) | 1730 |
| Eco53kI | GAGCTC | 1 cut(s) | 1969 |
| Eco57I | CTGAAG | 2 cut(s) | 343, 1493 |
| Eco88I | CYCGRG | 1 cut(s) | 1059 |
| EcoICRI | GAGCTC | 1 cut(s) | 1969 |
| EcoRI | GAATTC | 1 cut(s) | 1738 |
| EcoRII | CCWGG | 2 cut(s) | 125, 471 |
| EcoT14I | CCWWGG | 2 cut(s) | 255, 1042 |
| EcoT22I | ATGCAT | 1 cut(s) | 1488 |
| EcoT38I | GRGCYC | 1 cut(s) | 1971 |
| ErhI | CCWWGG | 2 cut(s) | 255, 1042 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 903, 935, 1627, 1659, 1765, 1797 |
| FaqI | GGGAC | 4 cut(s) | 125, 1091, 1666, 1818 |
| FauNDI | CATATG | 1 cut(s) | 820 |
| FbaI | TGATCA | 2 cut(s) | 297, 1251 |
| FokI | GGATG | 5 cut(s) | 272, 416, 533, 866, 1991 |
| FriOI | GRGCYC | 1 cut(s) | 1971 |
| FspBI | CTAG | 4 cut(s) | 978, 1785, 1842, 1901 |
| GsaI | CCCAGC | 1 cut(s) | 696 |
| GsuI | CTGGAG | 3 cut(s) | 1229, 1668, 1970 |
| HaeIII | GGCC | 2 cut(s) | 566, 1732 |
| HapII | CCGG | 1 cut(s) | 1177 |
| HincII | GTYRAC | 1 cut(s) | 1165 |
| HindII | GTYRAC | 1 cut(s) | 1165 |
| HindIII | AAGCTT | 1 cut(s) | 683 |
| HinfI | GANTC | 6 cut(s) | 14, 20, 113, 530, 1312, 1763 |
| HpaI | GTTAAC | 1 cut(s) | 1165 |
| HpaII | CCGG | 1 cut(s) | 1177 |
| HphI | GGTGA | 4 cut(s) | 475, 1298, 1358, 1607 |
| Hpy166II | GTNNAC | 4 cut(s) | 899, 1118, 1165, 1598 |
| Hpy188I | TCNGA | 9 cut(s) | 13, 19, 313, 735, 792, 1438, 1473, 1744, 1849 |
| Hpy8I | GTNNAC | 4 cut(s) | 899, 1118, 1165, 1598 |
| Hpy99I | CGWCG | 1 cut(s) | 28 |
| HpyAV | CCTTC | 7 cut(s) | 124, 197, 337, 394, 1148, 1345, 1520 |
| HpyCH4III | ACNGT | 5 cut(s) | 157, 412, 583, 1115, 1602 |
| HpyCH4IV | ACGT | 2 cut(s) | 1498, 1516 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 241, 575, 968, 1055, 1546 |
| HpyF3I | CTNAG | 8 cut(s) | 744, 903, 1332, 1586, 1605, 1853, 1878, 1883 |
| HpySE526I | ACGT | 2 cut(s) | 1498, 1516 |
| Ksp22I | TGATCA | 2 cut(s) | 297, 1251 |
| KspAI | GTTAAC | 1 cut(s) | 1165 |
| LguI | GCTCTTC | 1 cut(s) | 319 |
| LmnI | GCTCC | 5 cut(s) | 368, 1180, 1210, 1835, 1910 |
| Lsp1109I | GCAGC | 7 cut(s) | 415, 613, 676, 1015, 1331, 1552, 1679 |
| LweI | GCATC | 7 cut(s) | 250, 394, 631, 814, 964, 1045, 1222 |
| MaeI | CTAG | 4 cut(s) | 978, 1785, 1842, 1901 |
| MaeII | ACGT | 2 cut(s) | 1498, 1516 |
| MaeIII | GTNAC | 6 cut(s) | 68, 389, 481, 633, 833, 1355 |
| MbiI | CCGCTC | 1 cut(s) | 569 |
| MboII | GAAGA | 8 cut(s) | 233, 249, 265, 336, 349, 1504, 1747, 1810 |
| MflI | RGATCY | 3 cut(s) | 696, 779, 1216 |
| MhlI | GDGCHC | 4 cut(s) | 331, 1717, 1729, 1971 |
| MluI | ACGCGT | 1 cut(s) | 174 |
| MlyI | GAGTC | 2 cut(s) | 8, 107 |
| MmeI | TCCRAC | 3 cut(s) | 36, 336, 1664 |
| Mph1103I | ATGCAT | 1 cut(s) | 1488 |
| MroXI | GAANNNNTTC | 2 cut(s) | 90, 1896 |
| MseI | TTAA | 8 cut(s) | 159, 416, 596, 671, 845, 1164, 1625, 1772 |
| MslI | CAYNNNNRTG | 3 cut(s) | 650, 1044, 1720 |
| MspA1I | CMGCKG | 1 cut(s) | 1207 |
| MspI | CCGG | 1 cut(s) | 1177 |
| MspR9I | CCNGG | 3 cut(s) | 127, 473, 1178 |
| Mva1269I | GAATGC | 1 cut(s) | 1405 |
| MvaI | CCWGG | 2 cut(s) | 127, 473 |
| MvnI | CGCG | 2 cut(s) | 176, 1549 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 241, 575, 968, 1055, 1546 |
| NciI | CCSGG | 1 cut(s) | 1178 |
| NcoI | CCATGG | 2 cut(s) | 255, 1042 |
| NdeI | CATATG | 1 cut(s) | 820 |
| NlaIV | GGNNCC | 4 cut(s) | 879, 1716, 1837, 1906 |
| NmuCI | GTSAC | 6 cut(s) | 68, 389, 481, 633, 833, 1355 |
| NsiI | ATGCAT | 1 cut(s) | 1488 |
| NspI | RCATGY | 4 cut(s) | 319, 552, 759, 1781 |
| OliI | CACNNNNGTG | 1 cut(s) | 1044 |
| PaeI | GCATGC | 1 cut(s) | 1781 |
| PagI | TCATGA | 2 cut(s) | 711, 931 |
| PciI | ACATGT | 1 cut(s) | 315 |
| PciSI | GCTCTTC | 1 cut(s) | 319 |
| PctI | GAATGC | 1 cut(s) | 1405 |
| PdmI | GAANNNNTTC | 2 cut(s) | 90, 1896 |
| PfeI | GAWTC | 4 cut(s) | 20, 530, 1312, 1763 |
| PfoI | TCCNGGA | 1 cut(s) | 1176 |
| PleI | GAGTC | 2 cut(s) | 8, 107 |
| PpsI | GAGTC | 2 cut(s) | 8, 107 |
| PscI | ACATGT | 1 cut(s) | 315 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1635 |
| Psp124BI | GAGCTC | 1 cut(s) | 1971 |
| Psp6I | CCWGG | 2 cut(s) | 125, 471 |
| PspFI | CCCAGC | 1 cut(s) | 692 |
| PspGI | CCWGG | 2 cut(s) | 125, 471 |
| PspN4I | GGNNCC | 4 cut(s) | 879, 1716, 1837, 1906 |
| PspPI | GGNCC | 2 cut(s) | 96, 896 |
| PsuI | RGATCY | 3 cut(s) | 696, 779, 1216 |
| PvuII | CAGCTG | 1 cut(s) | 1207 |
| RsaI | GTAC | 1 cut(s) | 135 |
| RsaNI | GTAC | 1 cut(s) | 134 |
| RseI | CAYNNNNRTG | 3 cut(s) | 650, 1044, 1720 |
| SacI | GAGCTC | 1 cut(s) | 1971 |
| SapI | GCTCTTC | 1 cut(s) | 319 |
| SaqAI | TTAA | 8 cut(s) | 159, 416, 596, 671, 845, 1164, 1625, 1772 |
| Sau96I | GGNCC | 2 cut(s) | 96, 896 |
| SchI | GAGTC | 2 cut(s) | 8, 107 |
| ScrFI | CCNGG | 3 cut(s) | 127, 473, 1178 |
| SduI | GDGCHC | 4 cut(s) | 331, 1717, 1729, 1971 |
| SfaNI | GCATC | 7 cut(s) | 250, 394, 631, 814, 964, 1045, 1222 |
| SfcI | CTRYAG | 2 cut(s) | 462, 783 |
| SinI | GGWCC | 2 cut(s) | 96, 896 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 650, 1044, 1720 |
| SmlI | CTYRAG | 1 cut(s) | 947 |
| SmoI | CTYRAG | 1 cut(s) | 947 |
| SphI | GCATGC | 1 cut(s) | 1781 |
| SsiI | CCGC | 5 cut(s) | 36, 567, 616, 1106, 1300 |
| SspI | AATATT | 1 cut(s) | 455 |
| SspMI | CTAG | 4 cut(s) | 978, 1785, 1842, 1901 |
| SstI | GAGCTC | 1 cut(s) | 1971 |
| StyD4I | CCNGG | 3 cut(s) | 125, 471, 1176 |
| StyI | CCWWGG | 2 cut(s) | 255, 1042 |
| TaaI | ACNGT | 5 cut(s) | 157, 412, 583, 1115, 1602 |
| TaiI | ACGT | 2 cut(s) | 1501, 1519 |
| TaqI | TCGA | 2 cut(s) | 622, 1192 |
| TaqII | GACCGA | 2 cut(s) | 84, 87 |
| TauI | GCSGC | 3 cut(s) | 39, 569, 1108 |
| TfiI | GAWTC | 4 cut(s) | 20, 530, 1312, 1763 |
| Tru1I | TTAA | 8 cut(s) | 159, 416, 596, 671, 845, 1164, 1625, 1772 |
| Tru9I | TTAA | 8 cut(s) | 159, 416, 596, 671, 845, 1164, 1625, 1772 |
| TscAI | CASTG | 1 cut(s) | 1120 |
| TseFI | GTSAC | 6 cut(s) | 68, 389, 481, 633, 833, 1355 |
| TseI | GCWGC | 7 cut(s) | 428, 626, 689, 1028, 1319, 1540, 1667 |
| Tsp45I | GTSAC | 6 cut(s) | 68, 389, 481, 633, 833, 1355 |
| TspDTI | ATGAA | 9 cut(s) | 201, 458, 728, 825, 1000, 1130, 1256, 1382, 1412 |
| TspRI | CASTG | 1 cut(s) | 1120 |
| VpaK11BI | GGWCC | 2 cut(s) | 96, 896 |
| XapI | RAATTY | 6 cut(s) | 49, 445, 602, 1256, 1337, 1738 |
| XceI | RCATGY | 4 cut(s) | 319, 552, 759, 1781 |
| XmnI | GAANNNNTTC | 2 cut(s) | 90, 1896 |
| XspI | CTAG | 4 cut(s) | 978, 1785, 1842, 1901 |
| Zsp2I | ATGCAT | 1 cut(s) | 1488 |