RchiOBHm_Chr5g0038751

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001124
Physical Location & Seq
Reverse (-)
33527771 .. 33535657
7887 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31735

Sequence Viewer

Length: 1629 bp
ATGTATACAGTTATACACTTAGCTATCAAGATAGCTATCACTGTTGGAGATGCTCTTATACACCACCAACGGAAATTTGATTATTGCATGAAATGCTTCGGTCTTGCAAAGCCGGGAACTCAAAATCAACATGGAAGGTACAAATATGATCTATGTTTTTGTTTTTGTTTTTGCGGTGTTTTGGAGTATGAAAAGATTGTTATGTTGCTAGAGCGCATAGATAAGGAAAGGCAGCGATTTGCCTCCATGGATGCAATATATTTCATCCGGCCTTCTAAAGAGAATGTTAGATTCTTCTTGTCAGACATGTCGGGAAGTGCACCTTTATATAGGAAGGCGTTTGTTTTTTTCACTTCTCCTATATCGAAAGATTTGGTTGGGCAGATAAAGGGGGATGGAAGTCTAACAAGTCGCATATCTGCATTGAGTGAGGGTTTTGTTACCAATAATGAGAGAGCTTTAGCGCAGTTTTATGGGGACAAGGACGATTCACGCGAAGCCGAAGCGTGTTTGAGTGAGATGGCTGCACGTGTAGCCACAGTCTTTGCTTCTTTAAGGGAATTTCCTATTGTTCGCTACCGAGCTGCCAATAGGATTGATGCCACCATGGAGACCACTTTTCGTGATTTAATTCCTACAAAGCTTGCTGCTGGTGTCTGGGATTGTCTCATGCATTACAAAAGAACAATTCCAAATTTCCCTCAGATAGAAACATGCGAGTTACTTATCCTTGACAGATCTGTGGATCAGATTGCGCCAATCATCCACGAGTGGACATACGATGCCTTGTGTCATGACTTGTTAGATATGGAAGGAAATAAATATGTTGCGAGCATAAACGGTGGTCCTCCTGACAAGAAAGATGTTCTTCTAGAGGAGCGTGATCCTGTCTGGCTTGAACTTCGCGATCATCATTTAGGATTTACCAGCCTTATTGTGGAAGATAGCTTCAATTGTGCAATGGTTCTCAAATTTGACTTAGATGGGCAATGTCCTGAGCTATCTTCAAGGGAATTGAAAGAGATAACTGAAGCATTGCCACAGTATGTTGAACTAAAAGAAAAGCTCTCCCTTCATATAGAGCTGCGCTTGTTGATGATTCTTGCTGCCATCTATCCTGAGAAATTTGAGGATGAAGAAGATCTACATATGATGATGAAGTACGCATATTTACTACCCCAGGATATGACTGTTGTGAGGAATATGAAGTTGATTGGGGAGAGCAGAGAAGGCTCATCTGGAATTTTTCCTCTGAAGAAAGAGCGTCCAAGAGAAGATGAATGGCTTTATTCGCGCTTTTTTCCGATGATAGAGGAACTTATAGAAAAGCTTAGCAATGGTGCACTTTCAGAGGAATTATATCCATGTGTGAATCCCAACGCTCGTTTACTGAGATCGAGGAGGACACCAATGTGGGCCCACCGCCGGAATTCCGATAATGGGTTTTCAAGGGATGCATCTACTGATTTTGAAAAGATGGGCCGACGCATATTTGTAGTTATAGTAGGTGGAGCTACTAGATCAGAGCTTAGGGTTTGTCGGGAACTCACAGCAAAGCTCAAAAGGGAAGTTATTCTGGGCTCCTCATCTCTTGACGATCCTGCAACTTTTGTTACGGTATACTATTAG

Protein Analysis

542

Amino Acids

62.39

Weight (kDa)

5.86

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 72 - 362 1.9e-50 Sec1 family
Sec1 PF00995 362 - 538 3.6e-19 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 5, 1620
AccII CGCG 3 cut(s) 495, 906, 1294
AciI CCGC 2 cut(s) 174, 1423
AclWI GGATC 3 cut(s) 753, 878, 1592
AcsI RAATTY 7 cut(s) 74, 560, 694, 971, 1124, 1242, 1429
AcuI CTGAAG 2 cut(s) 1050, 1274
AcvI CACGTG 1 cut(s) 530
AfaI GTAC 2 cut(s) 140, 1163
AfiI CCNNNNNNNGG 3 cut(s) 937, 1425, 1440
AflIII ACRYGT 2 cut(s) 306, 529
AgsI TTSAA 7 cut(s) 899, 952, 1008, 1018, 1052, 1449, 1472
AjnI CCWGG 1 cut(s) 1179
AleI CACNNNNGTG 1 cut(s) 1411
Alw21I GWGCWC 2 cut(s) 322, 1345
Alw26I GTCTC 2 cut(s) 605, 671
Alw44I GTGCAC 2 cut(s) 318, 1341
AlwI GGATC 3 cut(s) 753, 878, 1592
AoxI GGCC 3 cut(s) 269, 1416, 1480
ApaI GGGCCC 1 cut(s) 1420
ApaLI GTGCAC 2 cut(s) 318, 1341
ApeKI GCWGC 6 cut(s) 232, 524, 584, 647, 1084, 1106
ApoI RAATTY 7 cut(s) 74, 560, 694, 971, 1124, 1242, 1429
Asp700I GAANNNNTTC 2 cut(s) 95, 1572
AspLEI GCGC 5 cut(s) 216, 466, 757, 1089, 1296
AspS9I GGNCC 4 cut(s) 845, 1416, 1417, 1480
AsuC2I CCSGG 1 cut(s) 114
AvaII GGWCC 1 cut(s) 845
BaeGI GKGCMC 3 cut(s) 322, 1345, 1420
BanII GRGCYC 2 cut(s) 1420, 1583
BarI GAAGNNNNNNTAC 2 cut(s) 1128, 1160
BauI CACGAG 1 cut(s) 767
BbrPI CACGTG 1 cut(s) 530
Bbv12I GWGCWC 2 cut(s) 322, 1345
BbvI GCAGC 6 cut(s) 244, 511, 571, 634, 1071, 1093
BccI CCATC 5 cut(s) 389, 514, 977, 1118, 1471
BciT130I CCWGG 1 cut(s) 1181
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 2 cut(s) 605, 671
BfaI CTAG 3 cut(s) 209, 872, 1518
BglII AGATCT 2 cut(s) 737, 1141
BisI GCNGC 6 cut(s) 233, 525, 585, 648, 1085, 1107
BlpI GCTNAGC 1 cut(s) 1331
BlsI GCNGC 6 cut(s) 234, 526, 586, 649, 1086, 1108
Bme1390I CCNGG 2 cut(s) 114, 1181
Bme18I GGWCC 1 cut(s) 845
BmgT120I GGNCC 4 cut(s) 845, 1416, 1417, 1480
BmiI GGNNCC 2 cut(s) 1418, 1582
BmrFI CCNGG 2 cut(s) 114, 1181
BmsI GCATC 6 cut(s) 40, 241, 589, 772, 1444, 1466
Bpu10I CCTNAGC 2 cut(s) 996, 1529
Bpu1102I GCTNAGC 1 cut(s) 1331
BpuMI CCSGG 1 cut(s) 114
BsaAI YACGTR 1 cut(s) 530
BsaBI GATNNNNATC 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 605
BsaJI CCNNGG 3 cut(s) 246, 606, 1179
Bsc4I CCNNNNNNNGG 3 cut(s) 937, 1425, 1440
Bse3DI GCAATG 4 cut(s) 966, 995, 1034, 1342
Bse8I GATNNNNATC 1 cut(s) 35
BseBI CCWGG 1 cut(s) 1181
BseDI CCNNGG 3 cut(s) 246, 606, 1179
BseGI GGATG 6 cut(s) 256, 264, 400, 762, 1138, 1459
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 3 cut(s) 937, 1425, 1440
BseMI GCAATG 4 cut(s) 966, 995, 1034, 1342
BseMII CTCAG 4 cut(s) 716, 987, 1110, 1382
BseRI GAGGAG 3 cut(s) 890, 1414, 1573
BseSI GKGCMC 3 cut(s) 322, 1345, 1420
BseXI GCAGC 6 cut(s) 244, 511, 571, 634, 1071, 1093
BsgI GTGCAG 1 cut(s) 510
Bsh1236I CGCG 3 cut(s) 495, 906, 1294
BshFI GGCC 3 cut(s) 271, 1418, 1482
BsiHKAI GWGCWC 2 cut(s) 322, 1345
BsiSI CCGG 3 cut(s) 113, 268, 1426
BslFI GGGAC 1 cut(s) 491
BslI CCNNNNNNNGG 3 cut(s) 937, 1425, 1440
BsmAI GTCTC 2 cut(s) 605, 671
BsmFI GGGAC 1 cut(s) 491
BsnI GGCC 3 cut(s) 271, 1418, 1482
Bso31I GGTCTC 1 cut(s) 605
Bsp120I GGGCCC 1 cut(s) 1416
Bsp1286I GDGCHC 4 cut(s) 322, 1345, 1420, 1583
Bsp143I GATC 9 cut(s) 148, 737, 745, 883, 907, 1141, 1394, 1520, 1597
Bsp1720I GCTNAGC 1 cut(s) 1331
Bsp19I CCATGG 2 cut(s) 246, 606
Bsp68I TCGCGA 1 cut(s) 906
BspACI CCGC 2 cut(s) 174, 1423
BspANI GGCC 3 cut(s) 271, 1418, 1482
BspCNI CTCAG 4 cut(s) 715, 988, 1111, 1383
BspFNI CGCG 3 cut(s) 495, 906, 1294
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 2 cut(s) 1418, 1582
BspPI GGATC 3 cut(s) 753, 878, 1592
BspTNI GGTCTC 1 cut(s) 605
BsrDI GCAATG 4 cut(s) 966, 995, 1034, 1342
BssECI CCNNGG 3 cut(s) 246, 606, 1179
BssMI GATC 9 cut(s) 148, 737, 745, 883, 907, 1141, 1394, 1520, 1597
BssNAI GTATAC 2 cut(s) 6, 1621
BssSI CACGAG 1 cut(s) 767
BssT1I CCWWGG 2 cut(s) 246, 606
Bst1107I GTATAC 2 cut(s) 6, 1621
Bst2BI CACGAG 1 cut(s) 767
Bst2UI CCWGG 1 cut(s) 1181
Bst4CI ACNGT 7 cut(s) 10, 43, 541, 842, 1044, 1192, 1618
BstAPI GCANNNNNTGC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 530
BstC8I GCNNGC 2 cut(s) 645, 832
BstDEI CTNAG 8 cut(s) 19, 702, 979, 996, 1119, 1331, 1391, 1529
BstDSI CCRYGG 2 cut(s) 246, 606
BstF5I GGATG 6 cut(s) 256, 264, 400, 762, 1138, 1459
BstFNI CGCG 3 cut(s) 495, 906, 1294
BstHHI GCGC 5 cut(s) 216, 466, 757, 1089, 1296
BstKTI GATC 9 cut(s) 151, 740, 748, 886, 910, 1144, 1397, 1523, 1600
BstMAI GTCTC 2 cut(s) 605, 671
BstMBI GATC 9 cut(s) 148, 737, 745, 883, 907, 1141, 1394, 1520, 1597
BstMWI GCNNNNNNNGC 4 cut(s) 93, 533, 1230, 1291
BstNI CCWGG 1 cut(s) 1181
BstNSI RCATGY 2 cut(s) 310, 717
BstSCI CCNGG 2 cut(s) 112, 1179
BstSLI GKGCMC 3 cut(s) 322, 1345, 1420
BstUI CGCG 3 cut(s) 495, 906, 1294
BstV1I GCAGC 6 cut(s) 244, 511, 571, 634, 1071, 1093
BstX2I RGATCY 2 cut(s) 737, 1141
BstYI RGATCY 2 cut(s) 737, 1141
BstZ17I GTATAC 2 cut(s) 6, 1621
BsuRI GGCC 3 cut(s) 271, 1418, 1482
BtgI CCRYGG 2 cut(s) 246, 606
BtsCI GGATG 6 cut(s) 256, 264, 400, 762, 1138, 1459
BtsIMutI CAGTG 1 cut(s) 39
BtuMI TCGCGA 1 cut(s) 906
Cac8I GCNNGC 2 cut(s) 645, 832
CciI TCATGA 1 cut(s) 793
CfoI GCGC 5 cut(s) 216, 466, 757, 1089, 1296
Cfr13I GGNCC 4 cut(s) 845, 1416, 1417, 1480
CseI GACGC 2 cut(s) 1253, 1494
Csp6I GTAC 2 cut(s) 139, 1162
CspCI CAANNNNNGTGG 2 cut(s) 526, 561
CviAII CATG 9 cut(s) 88, 131, 247, 307, 607, 670, 714, 794, 1365
CviQI GTAC 2 cut(s) 139, 1162
DdeI CTNAG 8 cut(s) 19, 702, 979, 996, 1119, 1331, 1391, 1529
DpnI GATC 9 cut(s) 150, 739, 747, 885, 909, 1143, 1396, 1522, 1599
DpnII GATC 9 cut(s) 148, 737, 745, 883, 907, 1141, 1394, 1520, 1597
Eco130I CCWWGG 2 cut(s) 246, 606
Eco24I GRGCYC 2 cut(s) 1420, 1583
Eco31I GGTCTC 1 cut(s) 605
Eco47I GGWCC 1 cut(s) 845
Eco57I CTGAAG 2 cut(s) 1050, 1274
Eco72I CACGTG 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 1429
EcoRII CCWGG 1 cut(s) 1179
EcoT14I CCWWGG 2 cut(s) 246, 606
EcoT22I ATGCAT 2 cut(s) 675, 1459
EcoT38I GRGCYC 2 cut(s) 1420, 1583
ErhI CCWWGG 2 cut(s) 246, 606
FaeI CATG 9 cut(s) 91, 134, 250, 310, 610, 673, 717, 797, 1368
FalI AAGNNNNNCTT 4 cut(s) 307, 339, 852, 884
FaqI GGGAC 1 cut(s) 491
FatI CATG 9 cut(s) 87, 130, 246, 306, 606, 669, 713, 793, 1364
FauNDI CATATG 1 cut(s) 1149
FblI GTMKAC 2 cut(s) 5, 1620
Fnu4HI GCNGC 6 cut(s) 233, 525, 585, 648, 1085, 1107
FokI GGATG 6 cut(s) 251, 263, 407, 749, 1145, 1466
FriOI GRGCYC 2 cut(s) 1420, 1583
Fsp4HI GCNGC 6 cut(s) 233, 525, 585, 648, 1085, 1107
FspBI CTAG 3 cut(s) 209, 872, 1518
GlaI GCGC 5 cut(s) 215, 465, 756, 1088, 1295
GluI GCNGC 6 cut(s) 233, 525, 585, 648, 1085, 1107
HaeIII GGCC 3 cut(s) 271, 1418, 1482
HapII CCGG 3 cut(s) 113, 268, 1426
HgaI GACGC 2 cut(s) 1253, 1494
HhaI GCGC 5 cut(s) 216, 466, 757, 1089, 1296
Hin1II CATG 9 cut(s) 91, 134, 250, 310, 610, 673, 717, 797, 1368
Hin6I GCGC 5 cut(s) 214, 464, 755, 1087, 1294
HinP1I GCGC 5 cut(s) 214, 464, 755, 1087, 1294
HindIII AAGCTT 2 cut(s) 641, 1328
HinfI GANTC 4 cut(s) 291, 488, 1099, 1372
HpaII CCGG 3 cut(s) 113, 268, 1426
Hpy166II GTNNAC 6 cut(s) 6, 320, 774, 1343, 1388, 1621
Hpy188I TCNGA 8 cut(s) 304, 705, 750, 1254, 1305, 1351, 1435, 1525
Hpy8I GTNNAC 6 cut(s) 6, 320, 774, 1343, 1388, 1621
Hpy99I CGWCG 1 cut(s) 1488
HpyAV CCTTC 6 cut(s) 129, 282, 328, 806, 1082, 1223
HpyCH4III ACNGT 7 cut(s) 10, 43, 541, 842, 1044, 1192, 1618
HpyCH4IV ACGT 1 cut(s) 529
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 533, 1230, 1291
HpyF3I CTNAG 8 cut(s) 19, 702, 979, 996, 1119, 1331, 1391, 1529
HpySE526I ACGT 1 cut(s) 529
Hsp92II CATG 9 cut(s) 91, 134, 250, 310, 610, 673, 717, 797, 1368
HspAI GCGC 5 cut(s) 214, 464, 755, 1087, 1294
Kzo9I GATC 9 cut(s) 148, 737, 745, 883, 907, 1141, 1394, 1520, 1597
LmnI GCTCC 3 cut(s) 877, 1511, 1586
Lsp1109I GCAGC 6 cut(s) 244, 511, 571, 634, 1071, 1093
LweI GCATC 6 cut(s) 40, 241, 589, 772, 1444, 1466
MaeI CTAG 3 cut(s) 209, 872, 1518
MaeII ACGT 1 cut(s) 529
MaeIII GTNAC 3 cut(s) 439, 720, 1612
MalI GATC 9 cut(s) 150, 739, 747, 885, 909, 1143, 1396, 1522, 1599
MboI GATC 9 cut(s) 148, 737, 745, 883, 907, 1141, 1394, 1520, 1597
MboII GAAGA 8 cut(s) 286, 860, 953, 996, 1148, 1151, 1267, 1286
MfeI CAATTG 1 cut(s) 952
MflI RGATCY 2 cut(s) 737, 1141
MhlI GDGCHC 4 cut(s) 322, 1345, 1420, 1583
MmeI TCCRAC 1 cut(s) 25
Mph1103I ATGCAT 2 cut(s) 675, 1459
MroXI GAANNNNTTC 2 cut(s) 95, 1572
MseI TTAA 2 cut(s) 554, 629
MslI CAYNNNNRTG 1 cut(s) 1411
MspI CCGG 3 cut(s) 113, 268, 1426
MspR9I CCNGG 2 cut(s) 114, 1181
MunI CAATTG 1 cut(s) 952
MvaI CCWGG 1 cut(s) 1181
MvnI CGCG 3 cut(s) 495, 906, 1294
MwoI GCNNNNNNNGC 4 cut(s) 93, 533, 1230, 1291
NciI CCSGG 1 cut(s) 114
NcoI CCATGG 2 cut(s) 246, 606
NdeI CATATG 1 cut(s) 1149
NdeII GATC 9 cut(s) 148, 737, 745, 883, 907, 1141, 1394, 1520, 1597
NlaIII CATG 9 cut(s) 91, 134, 250, 310, 610, 673, 717, 797, 1368
NlaIV GGNNCC 2 cut(s) 1418, 1582
NruI TCGCGA 1 cut(s) 906
NsiI ATGCAT 2 cut(s) 675, 1459
NspI RCATGY 2 cut(s) 310, 717
OliI CACNNNNGTG 1 cut(s) 1411
PagI TCATGA 1 cut(s) 793
PciI ACATGT 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PdmI GAANNNNTTC 2 cut(s) 95, 1572
PfeI GAWTC 4 cut(s) 291, 488, 1099, 1372
PkrI GCNGC 6 cut(s) 234, 526, 586, 649, 1086, 1108
PmaCI CACGTG 1 cut(s) 530
PmlI CACGTG 1 cut(s) 530
Ppu21I YACGTR 1 cut(s) 530
PscI ACATGT 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 1179
PspCI CACGTG 1 cut(s) 530
PspGI CCWGG 1 cut(s) 1179
PspN4I GGNNCC 2 cut(s) 1418, 1582
PspOMI GGGCCC 1 cut(s) 1416
PspPI GGNCC 4 cut(s) 845, 1416, 1417, 1480
PsuI RGATCY 2 cut(s) 737, 1141
RruI TCGCGA 1 cut(s) 906
RsaI GTAC 2 cut(s) 140, 1163
RsaNI GTAC 2 cut(s) 139, 1162
RseI CAYNNNNRTG 1 cut(s) 1411
SaqAI TTAA 2 cut(s) 554, 629
SatI GCNGC 6 cut(s) 233, 525, 585, 648, 1085, 1107
Sau3AI GATC 9 cut(s) 148, 737, 745, 883, 907, 1141, 1394, 1520, 1597
Sau96I GGNCC 4 cut(s) 845, 1416, 1417, 1480
ScrFI CCNGG 2 cut(s) 114, 1181
SduI GDGCHC 4 cut(s) 322, 1345, 1420, 1583
SfaNI GCATC 6 cut(s) 40, 241, 589, 772, 1444, 1466
SinI GGWCC 1 cut(s) 845
SmiMI CAYNNNNRTG 1 cut(s) 1411
SsiI CCGC 2 cut(s) 174, 1423
SspMI CTAG 3 cut(s) 209, 872, 1518
StyD4I CCNGG 2 cut(s) 112, 1179
StyI CCWWGG 2 cut(s) 246, 606
TaaI ACNGT 7 cut(s) 10, 43, 541, 842, 1044, 1192, 1618
TaiI ACGT 1 cut(s) 532
TaqI TCGA 2 cut(s) 365, 1397
TaqII GACCGA 1 cut(s) 89
TfiI GAWTC 4 cut(s) 291, 488, 1099, 1372
Tru1I TTAA 2 cut(s) 554, 629
Tru9I TTAA 2 cut(s) 554, 629
TscAI CASTG 1 cut(s) 46
TseI GCWGC 6 cut(s) 232, 524, 584, 647, 1084, 1106
TspDTI ATGAA 8 cut(s) 104, 204, 253, 1064, 1149, 1172, 1220, 1293
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 1 cut(s) 46
VneI GTGCAC 2 cut(s) 318, 1341
VpaK11BI GGWCC 1 cut(s) 845
XapI RAATTY 7 cut(s) 74, 560, 694, 971, 1124, 1242, 1429
XbaI TCTAGA 1 cut(s) 871
XceI RCATGY 2 cut(s) 310, 717
XcmI CCANNNNNNNNNTGG 1 cut(s) 934
XmiI GTMKAC 2 cut(s) 5, 1620
XmnI GAANNNNTTC 2 cut(s) 95, 1572
XspI CTAG 3 cut(s) 209, 872, 1518
Zsp2I ATGCAT 2 cut(s) 675, 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.