Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0001124

Physical Location & Seq

Genomic Coordinates Forward (+)

4795279 .. 4797076

Gene Structure 1798 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0063500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGGCGCAGAACCCATTTCGCTTCTTCGTCTTGACCTTCTCAGCTCTCATCTTCACTACCCTTTTACCTCCCACACTCTCCCGCACCGTAGTATCAGACACCACTGTTCTCGACGTCGCCGGTTCTCTCAGCCAGGCCCATGACGTCATCTCCTTCGACCCGCAAACCCTCAAGCCTTTGGACCACCAAGAAGCAGCTGAGCTACAACCACAAGCCCAAGCCCAGGCCCAGCTCAACTCCTCCTCGGTCTCGTCGCTCACTCTCCAGCTCCACCCCCGTGAGTCGCTCTACACCTCCCAGCACAAGGACTACAAGTCGTTGGTCCGCACCCGCCTCGCCCGCGACTCGGCCCGAGTCGACTCGCTCAACACCAAGCTCCAGTTTGCGCTGCAGGGGGTGAACCGGGCGGATCTGGAGCCCATGCAGACGGAGATCCGACCCGAGGATTTGTCCACTCCGGTTACTTCCGGGACCAGCATGGGAAGCGGCGAGTACTTCACACGCGTCGGCGTCGGAAACCCGGCGAAGCAGTTTTACATGGTGTTGGATACCGGAAGTGACGTCAATTGGCTCCAATGCCAGCCTTGCTCCGACTGTTACCAGCAAGCCGACCCGGTTTTCAACCCCACCGGATCCTCCACCTTCCGCCAGCTCTCGTGCGACGCGCCGCAATGCTCCGCTCTCCAGGTCTCCGCCTGCCGCAACGGCAAGTGCCTCTACCAGGTCTCCTACGGCGACGGCTCGTACACCGTCGGCGACTACGTCACCGAAACGGTGTCGTTCGGGAAGTCCGGCGCGATCAACACCGTCGCGATGGGATGCGGCCACGACAACGAGGGTTTGTTCGTCGCGGCCGCCGGACTGCTCGGACTCGGCGGCGGTTCTCTCTCACTTCCTTCGCAAGTTAAAGCTTCGTCGCTGTCGTACTGCCTCGTCAACCGCGACTCCAGCGCGTCGTCCACACTCGAGTTCAACTCTGCCGCGCCCCGCGACTCCGTCATCGCGCCGCTGCTGAAGAACCGCAAACTCGGAACCTTCTACTATGTCGGACTCACCGGGTTCAGCGTCGGCGGCCGTCCGGTGTCGATTCCGCCGTCTCTTTTTCAGATGGACGAAGCCGGCAACGGCGGGATCATCGTCGACTCGGGAACCGCCATCACTCGGCTGCAGACGGGGGCCTACAACGCGCTCCGCGACGCGTTCAAGCGACTCGCGCCTGACCTTACTCCCGCGGGCACCTTCGCGCTGTTCGACACGTGTTACGATCTCTCGGGAAAAACTAGCGTGCGGGTCCCAACGGTGTCGTTTCTATTTTCGGGTGGGAAAAGCTTTCCTCTCCCCGCCAAGAACTACTTGATTCCGGTGGACAACTCGGGCACTTTCTGCTTCGCCTTCGCTCCGACGTCGTCGGCTCTGTCCATCATCGGAAACGTCCAGCAGCAAGGGACACGTGTCAGTTACGACTTGGCGAACAACCGCGTCGGATTCTCTCCAAATAAGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005886 GO:0016020 GO:0019898 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04721 map04721

Pfam Domains

Protein Families

Sec1

Protein Analysis

501

Amino Acids

53.48

Weight (kDa)

6.23

Isoelectric Point (pI)

37.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	164 - 297	2.3e-09	Eukaryotic aspartyl protease
TAXi_N	PF14543	165 - 324	4e-46	Xylanase inhibitor N-terminal
TAXi_C	PF14541	347 - 497	4.5e-36	Xylanase inhibitor C-terminal
Asp	PF00026	377 - 498	2.6e-08	Eukaryotic aspartyl protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001124)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12360 AT4G12120
fragaria_vesca	FvH4_2g27020 FvH4_2g27020 FvH4_2g27020 FvH4_5g00770 FvH4_5g00770 FvH4_5g00770 FvH4_5g00770 FvH4_5g00770 FvH4_5g29992 FvH4_5g29992 FvH4_5g29992 FvH4_5g29992 FvH4_5g29992 FvH4_5g29992 FvH4_5g29992
malus_domestica	MD04G1079000.v1.1 MD06G1073800.v1.1
prunus_persica	Prupe.5G080200_v2.0.a1 Prupe.5G080200_v2.0.a1 Prupe.5G080200_v2.0.a1
pyrus_communis	pycom04g07080 pycom06g05270
rosa_chinensis	RchiOBHm_Chr5g0038751 RchiOBHm_Chr6g0295901 RchiOBHm_Chr7g0201411
rosa_laevigata	RLG00000003677 RLG00000011758 RLG00000033865
rosa_multiflora	Rmu_sc0001469.1_g000008 Rmu_sc0005722.1_g000002
rosa_roxburghii	Rroxscaffold_1G00042410 Rroxscaffold_3G00255300 Rroxscaffold_7G00172070
rosa_rugosa	Rorug05G0173300.1 Rorug05G0173400 Rorug05G0173500 Rorug06G0255000 Rorug06G0255000 Rorug07G0063400 Rorug07G0063500
rosa_samantha	Rh5AG260700 Rh5BG264400 Rh5CG297100 Rh5DG272800 Rh6AG368700 Rh6BG376400 Rh6CG382600 Rh6DG369200 Rh7AG190300 Rh7BG192700 Rh7CG201800 Rh7DG195300
rosa_wichuraiana	Rw5G024070 Rw5G024360 Rw6G032150 Rw7G016710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	4 cut(s)	117, 147, 564, 1406
AccB1I	GGYRCC	1 cut(s)	1235
AccBSI	CCGCTC	1 cut(s)	680
AccI	GTMKAC	2 cut(s)	357, 1140
AclWI	GGATC	5 cut(s)	417, 427, 627, 640, 1139
AcoI	YGGCCR	3 cut(s)	823, 852, 1072
AcuI	CTGAAG	1 cut(s)	1034
AcvI	CACGTG	2 cut(s)	1257, 1451
AcyI	GRCGYC	5 cut(s)	114, 144, 510, 561, 1403
AfaI	GTAC	3 cut(s)	494, 746, 926
AfiI	CCNNNNNNNGG	6 cut(s)	223, 304, 346, 442, 721, 1161
AflIII	ACRYGT	6 cut(s)	502, 1197, 1254, 1256, 1448, 1450
AgsI	TTSAA	3 cut(s)	622, 973, 1204
AhdI	GACNNNNNGTC	1 cut(s)	313
AjnI	CCWGG	4 cut(s)	132, 222, 684, 720
AleI	CACNNNNGTG	1 cut(s)	276
AluBI	AGCT	9 cut(s)	44, 197, 202, 232, 268, 376, 652, 911, 1329
AluI	AGCT	9 cut(s)	44, 197, 202, 232, 268, 376, 652, 911, 1329
Alw26I	GTCTC	4 cut(s)	253, 694, 730, 1101
AlwI	GGATC	5 cut(s)	417, 427, 627, 640, 1139
Ama87I	CYCGRG	6 cut(s)	351, 440, 965, 1144, 1270, 1372
AoxI	GGCC	7 cut(s)	135, 225, 348, 823, 852, 1072, 1176
ApeKI	GCWGC	5 cut(s)	194, 388, 1009, 1165, 1438
AspS9I	GGNCC	8 cut(s)	136, 181, 226, 322, 349, 471, 1176, 1291
AsuC2I	CCSGG	5 cut(s)	404, 469, 521, 614, 1057
AsuHPI	GGTGA	3 cut(s)	409, 757, 1045
AvaI	CYCGRG	6 cut(s)	351, 440, 965, 1144, 1270, 1372
AvaII	GGWCC	4 cut(s)	181, 322, 471, 1291
BaeGI	GKGCMC	2 cut(s)	1238, 1379
BaeI	ACNNNNGTAYC	2 cut(s)	75, 108
BamHI	GGATCC	1 cut(s)	632
BanI	GGYRCC	1 cut(s)	1235
BanII	GRGCYC	1 cut(s)	420
BarI	GAAGNNNNNNTAC	2 cut(s)	518, 550
BauI	CACGAG	1 cut(s)	655
BbrPI	CACGTG	2 cut(s)	1257, 1451
BbvI	GCAGC	5 cut(s)	206, 375, 996, 1152, 1450
BccI	CCATC	4 cut(s)	808, 1102, 1163, 1427
BceAI	ACGGC	6 cut(s)	721, 748, 754, 1059, 1078, 1141
BcgI	CGANNNNNNTGC	2 cut(s)	316, 350
BciT130I	CCWGG	4 cut(s)	134, 224, 686, 722
BciVI	GTATCC	1 cut(s)	541
BcnI	CCSGG	5 cut(s)	404, 469, 521, 614, 1057
BcoDI	GTCTC	4 cut(s)	253, 694, 730, 1101
BfaI	CTAG	1 cut(s)	1281
BfmI	CTRYAG	2 cut(s)	389, 1166
BfuI	GTATCC	1 cut(s)	541
BglI	GCCNNNNNGGC	1 cut(s)	705
BlpI	GCTNAGC	1 cut(s)	198
BmcAI	AGTACT	1 cut(s)	494
Bme1390I	CCNGG	9 cut(s)	134, 224, 404, 469, 521, 614, 686, 722, 1057
Bme18I	GGWCC	4 cut(s)	181, 322, 471, 1291
BmeRI	GACNNNNNGTC	1 cut(s)	313
BmeT110I	CYCGRG	6 cut(s)	351, 440, 965, 1144, 1270, 1372
BmgT120I	GGNCC	8 cut(s)	136, 181, 226, 322, 349, 471, 1176, 1291
BmrFI	CCNGG	9 cut(s)	134, 224, 404, 469, 521, 614, 686, 722, 1057
BmsI	GCATC	1 cut(s)	809
BoxI	GACNNNNGTC	1 cut(s)	1451
BplI	GAGNNNNNCTC	2 cut(s)	959, 991
BpmI	CTGGAG	5 cut(s)	248, 362, 434, 668, 931
Bpu1102I	GCTNAGC	1 cut(s)	198
BpuEI	CTTGAG	1 cut(s)	155
BpuMI	CCSGG	5 cut(s)	404, 469, 521, 614, 1057
BsaAI	YACGTR	2 cut(s)	1257, 1451
BsaHI	GRCGYC	5 cut(s)	114, 144, 510, 561, 1403
BsaI	GGTCTC	3 cut(s)	253, 694, 730
BsaJI	CCNNGG	4 cut(s)	222, 243, 441, 1230
BsaWI	WCCGGW	5 cut(s)	457, 551, 629, 1078, 1360
BsaXI	ACNNNNNCTCC	6 cut(s)	134, 164, 710, 740, 929, 959
Bsc4I	CCNNNNNNNGG	6 cut(s)	223, 304, 346, 442, 721, 1161
Bse118I	RCCGGY	2 cut(s)	119, 1118
Bse1I	ACTGG	1 cut(s)	379
Bse3DI	GCAATG	1 cut(s)	677
BseBI	CCWGG	4 cut(s)	134, 224, 686, 722
BseDI	CCNNGG	4 cut(s)	222, 243, 441, 1230
BseGI	GGATG	1 cut(s)	824
BseLI	CCNNNNNNNGG	6 cut(s)	223, 304, 346, 442, 721, 1161
BseMI	GCAATG	1 cut(s)	677
BseMII	CTCAG	3 cut(s)	54, 142, 189
BseNI	ACTGG	1 cut(s)	379
BseRI	GAGGAG	2 cut(s)	229, 232
BseSI	GKGCMC	2 cut(s)	1238, 1379
BseX3I	CGGCCG	2 cut(s)	852, 1072
BseXI	GCAGC	5 cut(s)	206, 375, 996, 1152, 1450
BseYI	CCCAGC	2 cut(s)	228, 297
Bsh1285I	CGRYCG	2 cut(s)	855, 1075
BshFI	GGCC	7 cut(s)	137, 227, 350, 825, 854, 1074, 1178
BshNI	GGYRCC	1 cut(s)	1235
BsiEI	CGRYCG	2 cut(s)	855, 1075
BsiHKCI	CYCGRG	6 cut(s)	351, 440, 965, 1144, 1270, 1372
BslFI	GGGAC	3 cut(s)	484, 1277, 1459
BslI	CCNNNNNNNGG	6 cut(s)	223, 304, 346, 442, 721, 1161
BsmAI	GTCTC	4 cut(s)	253, 694, 730, 1101
BsmBI	CGTCTC	1 cut(s)	1101
BsmFI	GGGAC	3 cut(s)	484, 1277, 1459
BsnI	GGCC	7 cut(s)	137, 227, 350, 825, 854, 1074, 1178
Bso31I	GGTCTC	3 cut(s)	253, 694, 730
BsoBI	CYCGRG	6 cut(s)	351, 440, 965, 1144, 1270, 1372
Bsp1286I	GDGCHC	3 cut(s)	420, 1238, 1379
Bsp143I	GATC	6 cut(s)	409, 432, 632, 798, 1131, 1264
Bsp1720I	GCTNAGC	1 cut(s)	198
Bsp68I	TCGCGA	1 cut(s)	812
BspANI	GGCC	7 cut(s)	137, 227, 350, 825, 854, 1074, 1178
BspCNI	CTCAG	3 cut(s)	53, 141, 190
BspMAI	CTGCAG	2 cut(s)	393, 1170
BspPI	GGATC	5 cut(s)	417, 427, 627, 640, 1139
BspT107I	GGYRCC	1 cut(s)	1235
BspTNI	GGTCTC	3 cut(s)	253, 694, 730
BsrBI	CCGCTC	1 cut(s)	680
BsrDI	GCAATG	1 cut(s)	677
BsrFI	RCCGGY	2 cut(s)	119, 1118
BsrI	ACTGG	1 cut(s)	379
BssAI	RCCGGY	2 cut(s)	119, 1118
BssECI	CCNNGG	4 cut(s)	222, 243, 441, 1230
BssMI	GATC	6 cut(s)	409, 432, 632, 798, 1131, 1264
BssNI	GRCGYC	5 cut(s)	114, 144, 510, 561, 1403
BssSI	CACGAG	1 cut(s)	655
Bst2BI	CACGAG	1 cut(s)	655
Bst2UI	CCWGG	4 cut(s)	134, 224, 686, 722
Bst4CI	ACNGT	7 cut(s)	88, 106, 596, 751, 775, 808, 1300
BstACI	GRCGYC	5 cut(s)	114, 144, 510, 561, 1403
BstAPI	GCANNNNNTGC	1 cut(s)	1383
BstBAI	YACGTR	2 cut(s)	1257, 1451
BstC8I	GCNNGC	8 cut(s)	340, 581, 606, 650, 697, 1120, 1234, 1286
BstDEI	CTNAG	3 cut(s)	40, 128, 198
BstDSI	CCRYGG	1 cut(s)	1230
BstENI	CCTNNNNNAGG	1 cut(s)	719
BstF5I	GGATG	1 cut(s)	824
BstKTI	GATC	6 cut(s)	412, 435, 635, 801, 1134, 1267
BstMAI	GTCTC	4 cut(s)	253, 694, 730, 1101
BstMBI	GATC	6 cut(s)	409, 432, 632, 798, 1131, 1264
BstMCI	CGRYCG	2 cut(s)	855, 1075
BstMWI	GCNNNNNNNGC	9 cut(s)	339, 483, 585, 705, 948, 1071, 1184, 1213, 1383
BstNI	CCWGG	4 cut(s)	134, 224, 686, 722
BstPAI	GACNNNNGTC	1 cut(s)	1451
BstSCI	CCNGG	9 cut(s)	132, 222, 402, 467, 519, 612, 684, 720, 1055
BstSFI	CTRYAG	2 cut(s)	389, 1166
BstSLI	GKGCMC	2 cut(s)	1238, 1379
BstV1I	GCAGC	5 cut(s)	206, 375, 996, 1152, 1450
BstX2I	RGATCY	3 cut(s)	409, 432, 632
BstYI	RGATCY	3 cut(s)	409, 432, 632
BstZI	CGGCCG	2 cut(s)	852, 1072
BsuI	GTATCC	1 cut(s)	541
BsuRI	GGCC	7 cut(s)	137, 227, 350, 825, 854, 1074, 1178
BtgI	CCRYGG	1 cut(s)	1230
BtgZI	GCGATG	2 cut(s)	827, 985
BtsCI	GGATG	1 cut(s)	824
BtsIMutI	CAGTG	1 cut(s)	102
BtuMI	TCGCGA	1 cut(s)	812
Cac8I	GCNNGC	8 cut(s)	340, 581, 606, 650, 697, 1120, 1234, 1286
CciNI	GCGGCCGC	1 cut(s)	852
Cfr10I	RCCGGY	2 cut(s)	119, 1118
Cfr13I	GGNCC	8 cut(s)	136, 181, 226, 322, 349, 471, 1176, 1291
Cfr42I	CCGCGG	1 cut(s)	1233
CseI	GACGC	7 cut(s)	493, 499, 671, 942, 1054, 1205, 1468
CsiI	ACCWGGT	1 cut(s)	720
Csp6I	GTAC	3 cut(s)	493, 745, 925
CviAII	CATG	4 cut(s)	140, 421, 478, 538
CviQI	GTAC	3 cut(s)	493, 745, 925
DdeI	CTNAG	3 cut(s)	40, 128, 198
DpnI	GATC	6 cut(s)	411, 434, 634, 800, 1133, 1266
DpnII	GATC	6 cut(s)	409, 432, 632, 798, 1131, 1264
DriI	GACNNNNNGTC	1 cut(s)	313
EaeI	YGGCCR	3 cut(s)	823, 852, 1072
EagI	CGGCCG	2 cut(s)	852, 1072
Eam1105I	GACNNNNNGTC	1 cut(s)	313
EciI	GGCGGA	4 cut(s)	422, 635, 682, 1080
EclXI	CGGCCG	2 cut(s)	852, 1072
Eco24I	GRGCYC	1 cut(s)	420
Eco31I	GGTCTC	3 cut(s)	253, 694, 730
Eco47I	GGWCC	4 cut(s)	181, 322, 471, 1291
Eco52I	CGGCCG	2 cut(s)	852, 1072
Eco57I	CTGAAG	1 cut(s)	1034
Eco72I	CACGTG	2 cut(s)	1257, 1451
Eco88I	CYCGRG	6 cut(s)	351, 440, 965, 1144, 1270, 1372
EcoNI	CCTNNNNNAGG	1 cut(s)	719
EcoO109I	RGGNCCY	2 cut(s)	1176, 1291
EcoRII	CCWGG	4 cut(s)	132, 222, 684, 720
EcoT38I	GRGCYC	1 cut(s)	420
Esp3I	CGTCTC	1 cut(s)	1101
FaeI	CATG	4 cut(s)	143, 424, 481, 541
FaiI	YATR	5 cut(s)	141, 422, 479, 539, 1044
FalI	AAGNNNNNCTT	2 cut(s)	1337, 1369
FaqI	GGGAC	3 cut(s)	484, 1277, 1459
FatI	CATG	4 cut(s)	139, 420, 477, 537
FblI	GTMKAC	2 cut(s)	357, 1140
FokI	GGATG	1 cut(s)	831
FriOI	GRGCYC	1 cut(s)	420
FspBI	CTAG	1 cut(s)	1281
GsaI	CCCAGC	2 cut(s)	232, 301
GsuI	CTGGAG	5 cut(s)	248, 362, 434, 668, 931
HaeIII	GGCC	7 cut(s)	137, 227, 350, 825, 854, 1074, 1178
HgaI	GACGC	7 cut(s)	493, 499, 671, 942, 1054, 1205, 1468
Hin1I	GRCGYC	5 cut(s)	114, 144, 510, 561, 1403
Hin1II	CATG	4 cut(s)	143, 424, 481, 541
HincII	GTYRAC	3 cut(s)	358, 937, 1141
HindII	GTYRAC	3 cut(s)	358, 937, 1141
HindIII	AAGCTT	2 cut(s)	909, 1327
HphI	GGTGA	3 cut(s)	409, 757, 1045
Hpy166II	GTNNAC	8 cut(s)	358, 400, 453, 747, 937, 960, 1141, 1366
Hpy188III	TCNNGA	7 cut(s)	31, 110, 413, 784, 811, 1146, 1272
Hpy8I	GTNNAC	8 cut(s)	358, 400, 453, 747, 937, 960, 1141, 1366
HpyAV	CCTTC	7 cut(s)	46, 163, 652, 906, 1045, 1249, 1402
HpyCH4III	ACNGT	7 cut(s)	88, 106, 596, 751, 775, 808, 1300
HpyCH4IV	ACGT	8 cut(s)	114, 144, 561, 762, 1256, 1403, 1431, 1450
HpyCH4V	TGCA	3 cut(s)	391, 424, 1168
HpyF10VI	GCNNNNNNNGC	9 cut(s)	339, 483, 585, 705, 948, 1071, 1184, 1213, 1383
HpyF3I	CTNAG	3 cut(s)	40, 128, 198
HpySE526I	ACGT	8 cut(s)	114, 144, 561, 762, 1256, 1403, 1431, 1450
Hsp92I	GRCGYC	5 cut(s)	114, 144, 510, 561, 1403
Hsp92II	CATG	4 cut(s)	143, 424, 481, 541
KflI	GGGWCCC	1 cut(s)	1291
KroI	GCCGGC	1 cut(s)	1118
KroNI	GCCGGC	1 cut(s)	1120
KspI	CCGCGG	1 cut(s)	1233
Kzo9I	GATC	6 cut(s)	409, 432, 632, 798, 1131, 1264
LmnI	GCTCC	8 cut(s)	273, 381, 415, 576, 593, 680, 1194, 1402
Lsp1109I	GCAGC	5 cut(s)	206, 375, 996, 1152, 1450
LweI	GCATC	1 cut(s)	809
MabI	ACCWGGT	1 cut(s)	720
MaeI	CTAG	1 cut(s)	1281
MaeII	ACGT	8 cut(s)	114, 144, 561, 762, 1256, 1403, 1431, 1450
MaeIII	GTNAC	6 cut(s)	460, 557, 596, 763, 1259, 1457
MalI	GATC	6 cut(s)	411, 434, 634, 800, 1133, 1266
MbiI	CCGCTC	1 cut(s)	680
MboI	GATC	6 cut(s)	409, 432, 632, 798, 1131, 1264
MboII	GAAGA	3 cut(s)	16, 43, 1027
MfeI	CAATTG	1 cut(s)	565
MflI	RGATCY	3 cut(s)	409, 432, 632
MhlI	GDGCHC	3 cut(s)	420, 1238, 1379
MluCI	AATT	1 cut(s)	565
MluI	ACGCGT	2 cut(s)	502, 1197
MmeI	TCCRAC	7 cut(s)	460, 493, 525, 615, 1027, 1424, 1462
MroNI	GCCGGC	1 cut(s)	1118
MseI	TTAA	2 cut(s)	906, 1504
MslI	CAYNNNNRTG	1 cut(s)	276
MspA1I	CMGCKG	3 cut(s)	197, 1009, 1232
MspR9I	CCNGG	9 cut(s)	134, 224, 404, 469, 521, 614, 686, 722, 1057
MunI	CAATTG	1 cut(s)	565
MvaI	CCWGG	4 cut(s)	134, 224, 686, 722
MwoI	GCNNNNNNNGC	9 cut(s)	339, 483, 585, 705, 948, 1071, 1184, 1213, 1383
NaeI	GCCGGC	1 cut(s)	1120
NciI	CCSGG	5 cut(s)	404, 469, 521, 614, 1057
NdeII	GATC	6 cut(s)	409, 432, 632, 798, 1131, 1264
NgoMIV	GCCGGC	1 cut(s)	1118
NlaIII	CATG	4 cut(s)	143, 424, 481, 541
NmeAIII	GCCGAG	3 cut(s)	326, 852, 1141
NmuCI	GTSAC	2 cut(s)	557, 763
NotI	GCGGCCGC	1 cut(s)	852
NruI	TCGCGA	1 cut(s)	812
OliI	CACNNNNGTG	1 cut(s)	276
PaeR7I	CTCGAG	1 cut(s)	965
PcsI	WCGNNNNNNNCGW	6 cut(s)	759, 920, 939, 1091, 1191, 1248
PdiI	GCCGGC	1 cut(s)	1120
PfeI	GAWTC	3 cut(s)	1087, 1357, 1485
PflFI	GACNNNGTC	3 cut(s)	761, 995, 1405
PfoI	TCCNGGA	1 cut(s)	467
PmaCI	CACGTG	2 cut(s)	1257, 1451
PmlI	CACGTG	2 cut(s)	1257, 1451
Ppu21I	YACGTR	2 cut(s)	1257, 1451
PpuMI	RGGWCCY	1 cut(s)	1291
PshAI	GACNNNNGTC	1 cut(s)	1451
Psp5II	RGGWCCY	1 cut(s)	1291
Psp6I	CCWGG	4 cut(s)	132, 222, 684, 720
PspCI	CACGTG	2 cut(s)	1257, 1451
PspFI	CCCAGC	2 cut(s)	228, 297
PspGI	CCWGG	4 cut(s)	132, 222, 684, 720
PspPI	GGNCC	8 cut(s)	136, 181, 226, 322, 349, 471, 1176, 1291
PspPPI	RGGWCCY	1 cut(s)	1291
PspXI	VCTCGAGB	1 cut(s)	965
PstI	CTGCAG	2 cut(s)	393, 1170
PsuI	RGATCY	3 cut(s)	409, 432, 632
PsyI	GACNNNGTC	3 cut(s)	761, 995, 1405
PvuII	CAGCTG	1 cut(s)	197
RruI	TCGCGA	1 cut(s)	812
RsaI	GTAC	3 cut(s)	494, 746, 926
RsaNI	GTAC	3 cut(s)	493, 745, 925
RseI	CAYNNNNRTG	1 cut(s)	276
SacII	CCGCGG	1 cut(s)	1233
SalI	GTCGAC	2 cut(s)	356, 1139
SaqAI	TTAA	2 cut(s)	906, 1504
Sau3AI	GATC	6 cut(s)	409, 432, 632, 798, 1131, 1264
Sau96I	GGNCC	8 cut(s)	136, 181, 226, 322, 349, 471, 1176, 1291
ScaI	AGTACT	1 cut(s)	494
ScrFI	CCNGG	9 cut(s)	134, 224, 404, 469, 521, 614, 686, 722, 1057
SduI	GDGCHC	3 cut(s)	420, 1238, 1379
SexAI	ACCWGGT	1 cut(s)	720
SfaNI	GCATC	1 cut(s)	809
SfcI	CTRYAG	2 cut(s)	389, 1166
Sfr274I	CTCGAG	1 cut(s)	965
Sfr303I	CCGCGG	1 cut(s)	1233
SgrBI	CCGCGG	1 cut(s)	1233
SinI	GGWCC	4 cut(s)	181, 322, 471, 1291
SlaI	CTCGAG	1 cut(s)	965
SmiMI	CAYNNNNRTG	1 cut(s)	276
SmlI	CTYRAG	2 cut(s)	170, 965
SmoI	CTYRAG	2 cut(s)	170, 965
Sse9I	AATT	1 cut(s)	565
SspMI	CTAG	1 cut(s)	1281
StyD4I	CCNGG	9 cut(s)	132, 222, 402, 467, 519, 612, 684, 720, 1055
TaaI	ACNGT	7 cut(s)	88, 106, 596, 751, 775, 808, 1300
TaiI	ACGT	8 cut(s)	117, 147, 564, 765, 1259, 1406, 1434, 1453
TaqI	TCGA	7 cut(s)	111, 156, 357, 966, 1085, 1140, 1251
TaqII	GACCGA	1 cut(s)	235
TasI	AATT	1 cut(s)	565
TatI	WGTACW	1 cut(s)	492
TfiI	GAWTC	3 cut(s)	1087, 1357, 1485
Tru1I	TTAA	2 cut(s)	906, 1504
Tru9I	TTAA	2 cut(s)	906, 1504
TscAI	CASTG	1 cut(s)	109
TseFI	GTSAC	2 cut(s)	557, 763
TseI	GCWGC	5 cut(s)	194, 388, 1009, 1165, 1438
Tsp45I	GTSAC	2 cut(s)	557, 763
TspGWI	ACGGA	2 cut(s)	443, 985
TspRI	CASTG	1 cut(s)	109
Tth111I	GACNNNGTC	3 cut(s)	761, 995, 1405
VpaK11BI	GGWCC	4 cut(s)	181, 322, 471, 1291
XagI	CCTNNNNNAGG	1 cut(s)	719
XhoI	CTCGAG	1 cut(s)	965
XmiI	GTMKAC	2 cut(s)	357, 1140
XspI	CTAG	1 cut(s)	1281
ZraI	GACGTC	4 cut(s)	115, 145, 562, 1404
ZrmI	AGTACT	1 cut(s)	494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.