pycom01g10170

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002430
Physical Location & Seq
Forward (+)
10994673 .. 10996272
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 783 bp
ATGGCCGATGAGAAGGAGCACGCTGGATCGCCGGTGGAGGATAAGCCATCGCTGTTGGAGAAGATAGGCGACAAGATCCACGTCGGTGATCACGATTCGTCGTCTTCCTCGTCTTCCGACTCGGATAACGAGAAATCGGAGAAGAAGACCGAGTCGGTGAAGGCCAAGGTTTTCCGGATTTTCGGCCGTGAGAGGCCGGTTCACCATGTGCTCGGCGGTGGAAAGCCTGCTGATGTGTTCTTGTGGAGAAACAAGAAGGTTTCGGCTGGAGTTCTCGGCGGAACCACCGCCATCTGGGTCCTCTTCGAGTTGCTTGAGTACCACCTGCTTACACTTGTTGGCCACATTCTAATTGGTGCCCTGGTAATCTTGTTCCTCTGGTCCAATGCCCATGCGTTTATCCACAAGACTCCCCCTCATATCCCGAAAGTTCACCTTCCTGAAGAACCATTCCTGCAGGTTGCATCTGAGCTGAGAGTTCAAATCAACTGTGCATTTGCACTGCTTCATGATATTGCATCTGGGAGAGACTTGAAAAAATTCCTCGCTGTTGTTGCTGGCTTGTGGGTTCTATCTGTTGTGGGCAAATGGTGCAACTTCCTGACATTGTTTTACATATGCTTTGTTTTGCTGCACACCGTGCCCGTCATTTATGAGAAGTACGAAGACAAGATCGACCCGCTTGCAGAGAAGGCGACTATCGAGTTCAAAAAGCAGTATGCCGTGTTTGATGCCAAGGTTTTGAGTAAGATTCCGCATGGTCTAAAAAGCAAGAGGTCTTAG

Protein Analysis

261

Amino Acids

29.03

Weight (kDa)

8.6

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 77 - 232 2.4e-60 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 333
Acc36I ACCTGC 2 cut(s) 333, 448
AccB1I GGYRCC 1 cut(s) 356
AccIII TCCGGA 1 cut(s) 174
AciI CCGC 5 cut(s) 216, 279, 288, 680, 755
AclWI GGATC 2 cut(s) 34, 70
AcoI YGGCCR 3 cut(s) 3, 184, 340
AcsI RAATTY 1 cut(s) 539
AcuI CTGAAG 1 cut(s) 462
AdeI CACNNNGTG 2 cut(s) 208, 640
AfaI GTAC 2 cut(s) 320, 662
AfiI CCNNNNNNNGG 1 cut(s) 294
AgsI TTSAA 3 cut(s) 482, 535, 709
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 1 cut(s) 360
AleI CACNNNNGTG 1 cut(s) 84
AluBI AGCT 1 cut(s) 472
AluI AGCT 1 cut(s) 472
Alw21I GWGCWC 2 cut(s) 21, 213
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 2 cut(s) 34, 70
Aor13HI TCCGGA 1 cut(s) 174
AoxI GGCC 5 cut(s) 3, 162, 184, 194, 340
ApeKI GCWGC 1 cut(s) 631
ApoI RAATTY 1 cut(s) 539
Asp700I GAANNNNTTC 1 cut(s) 539
AspS9I GGNCC 2 cut(s) 298, 381
AsuHPI GGTGA 4 cut(s) 98, 169, 194, 425
AvaII GGWCC 2 cut(s) 298, 381
BaeGI GKGCMC 2 cut(s) 361, 645
BalI TGGCCA 1 cut(s) 342
BanI GGYRCC 1 cut(s) 356
BbsI GAAGAC 4 cut(s) 96, 105, 152, 672
Bbv12I GWGCWC 2 cut(s) 21, 213
BbvI GCAGC 1 cut(s) 618
BccI CCATC 2 cut(s) 55, 299
BceAI ACGGC 2 cut(s) 171, 707
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BciT130I CCWGG 1 cut(s) 362
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 455
BfuAI ACCTGC 2 cut(s) 333, 448
BisI GCNGC 1 cut(s) 632
BlsI GCNGC 1 cut(s) 633
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 2 cut(s) 298, 381
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 298, 381
BmiI GGNNCC 3 cut(s) 283, 299, 358
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 3 cut(s) 473, 527, 721
BpiI GAAGAC 4 cut(s) 96, 105, 152, 672
BpmI CTGGAG 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 335
BsaJI CCNNGG 3 cut(s) 165, 360, 735
BsaWI WCCGGW 1 cut(s) 174
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse118I RCCGGY 2 cut(s) 31, 196
BseAI TCCGGA 1 cut(s) 174
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 165, 360, 735
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 2 cut(s) 459, 464
BseSI GKGCMC 2 cut(s) 361, 645
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 1 cut(s) 618
BsgI GTGCAG 1 cut(s) 617
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 5 cut(s) 5, 164, 186, 196, 342
BshNI GGYRCC 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 2 cut(s) 21, 213
BsiSI CCGG 3 cut(s) 32, 175, 197
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 522
BsnI GGCC 5 cut(s) 5, 164, 186, 196, 342
Bsp1286I GDGCHC 4 cut(s) 21, 213, 361, 645
Bsp13I TCCGGA 1 cut(s) 174
Bsp143I GATC 4 cut(s) 26, 75, 88, 672
BspACI CCGC 5 cut(s) 216, 279, 288, 680, 755
BspANI GGCC 5 cut(s) 5, 164, 186, 196, 342
BspCNI CTCAG 2 cut(s) 460, 465
BspEI TCCGGA 1 cut(s) 174
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 283, 299, 358
BspMAI CTGCAG 1 cut(s) 459
BspMI ACCTGC 2 cut(s) 333, 448
BspPI GGATC 2 cut(s) 34, 70
BspT107I GGYRCC 1 cut(s) 356
BsrFI RCCGGY 2 cut(s) 31, 196
BssAI RCCGGY 2 cut(s) 31, 196
BssECI CCNNGG 3 cut(s) 165, 360, 735
BssMI GATC 4 cut(s) 26, 75, 88, 672
BssT1I CCWWGG 2 cut(s) 165, 735
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 491, 640
Bst6I CTCTTC 1 cut(s) 308
BstAPI GCANNNNNTGC 2 cut(s) 591, 640
BstC8I GCNNGC 4 cut(s) 21, 228, 559, 684
BstDEI CTNAG 3 cut(s) 468, 473, 780
BstKTI GATC 4 cut(s) 29, 78, 91, 675
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 4 cut(s) 26, 75, 88, 672
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 4 cut(s) 554, 591, 640, 692
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
BstSFI CTRYAG 1 cut(s) 455
BstSLI GKGCMC 2 cut(s) 361, 645
BstV1I GCAGC 1 cut(s) 618
BstV2I GAAGAC 4 cut(s) 96, 105, 152, 672
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BstZI CGGCCG 1 cut(s) 184
BsuRI GGCC 5 cut(s) 5, 164, 186, 196, 342
BtgZI GCGATG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 82
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 1 cut(s) 500
BveI ACCTGC 2 cut(s) 333, 448
Cac8I GCNNGC 4 cut(s) 21, 228, 559, 684
CciI TCATGA 1 cut(s) 508
Cfr10I RCCGGY 2 cut(s) 31, 196
Cfr13I GGNCC 2 cut(s) 298, 381
Csp6I GTAC 2 cut(s) 319, 661
CviAII CATG 4 cut(s) 206, 392, 509, 758
CviQI GTAC 2 cut(s) 319, 661
DdeI CTNAG 3 cut(s) 468, 473, 780
DpnI GATC 4 cut(s) 28, 77, 90, 674
DpnII GATC 4 cut(s) 26, 75, 88, 672
DraIII CACNNNGTG 2 cut(s) 208, 640
EaeI YGGCCR 3 cut(s) 3, 184, 340
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
EciI GGCGGA 1 cut(s) 294
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 2 cut(s) 165, 735
Eco47I GGWCC 2 cut(s) 298, 381
Eco52I CGGCCG 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 462
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 2 cut(s) 165, 735
ErhI CCWWGG 2 cut(s) 165, 735
FaeI CATG 4 cut(s) 209, 395, 512, 761
FaiI YATR 9 cut(s) 207, 393, 420, 510, 617, 619, 654, 720, 759
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FatI CATG 4 cut(s) 205, 391, 508, 757
FauI CCCGC 1 cut(s) 687
FauNDI CATATG 1 cut(s) 617
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 1 cut(s) 632
Fsp4HI GCNGC 1 cut(s) 632
GluI GCNGC 1 cut(s) 632
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 5 cut(s) 5, 164, 186, 196, 342
HapII CCGG 3 cut(s) 32, 175, 197
Hin1II CATG 4 cut(s) 209, 395, 512, 761
HinfI GANTC 5 cut(s) 95, 119, 152, 409, 751
HpaII CCGG 3 cut(s) 32, 175, 197
HphI GGTGA 4 cut(s) 98, 169, 194, 425
Hpy166II GTNNAC 2 cut(s) 202, 433
Hpy188I TCNGA 4 cut(s) 118, 124, 139, 469
Hpy188III TCNNGA 6 cut(s) 92, 175, 424, 440, 509, 601
Hpy8I GTNNAC 2 cut(s) 202, 433
Hpy99I CGWCG 2 cut(s) 86, 103
HpyAV CCTTC 5 cut(s) 7, 154, 250, 446, 685
HpyCH4III ACNGT 2 cut(s) 491, 640
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 8 cut(s) 457, 464, 494, 500, 518, 594, 634, 686
HpyF10VI GCNNNNNNNGC 4 cut(s) 554, 591, 640, 692
HpyF3I CTNAG 3 cut(s) 468, 473, 780
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 4 cut(s) 209, 395, 512, 761
Kpn2I TCCGGA 1 cut(s) 174
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 4 cut(s) 26, 75, 88, 672
LmnI GCTCC 1 cut(s) 16
Lsp1109I GCAGC 1 cut(s) 618
LweI GCATC 3 cut(s) 473, 527, 721
MaeII ACGT 1 cut(s) 81
MalI GATC 4 cut(s) 28, 77, 90, 674
MboI GATC 4 cut(s) 26, 75, 88, 672
MboII GAAGA 8 cut(s) 73, 96, 105, 154, 157, 295, 455, 677
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 4 cut(s) 21, 213, 361, 645
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 2 cut(s) 351, 539
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 3 cut(s) 113, 161, 403
MmeI TCCRAC 2 cut(s) 36, 141
MnlI CCTC 8 cut(s) 31, 118, 186, 311, 386, 426, 554, 768
Mox20I TGGCCA 1 cut(s) 342
MroI TCCGGA 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 539
MscI TGGCCA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 84
Msp20I TGGCCA 1 cut(s) 342
MspI CCGG 3 cut(s) 32, 175, 197
MspR9I CCNGG 1 cut(s) 362
MvaI CCWGG 1 cut(s) 362
MwoI GCNNNNNNNGC 4 cut(s) 554, 591, 640, 692
NdeI CATATG 1 cut(s) 617
NdeII GATC 4 cut(s) 26, 75, 88, 672
NlaIII CATG 4 cut(s) 209, 395, 512, 761
NlaIV GGNNCC 3 cut(s) 283, 299, 358
NmeAIII GCCGAG 2 cut(s) 192, 255
OliI CACNNNNGTG 1 cut(s) 84
PagI TCATGA 1 cut(s) 508
PaqCI CACCTGC 1 cut(s) 333
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 107
PdmI GAANNNNTTC 1 cut(s) 539
PfeI GAWTC 2 cut(s) 95, 751
PflFI GACNNNGTC 1 cut(s) 151
PkrI GCNGC 1 cut(s) 633
PleI GAGTC 3 cut(s) 113, 160, 403
PpsI GAGTC 3 cut(s) 113, 160, 403
PpuMI RGGWCCY 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 283, 299, 358
PspPI GGNCC 2 cut(s) 298, 381
PspPPI RGGWCCY 1 cut(s) 298
PstI CTGCAG 1 cut(s) 459
PsuI RGATCY 1 cut(s) 75
PsyI GACNNNGTC 1 cut(s) 151
RsaI GTAC 2 cut(s) 320, 662
RsaNI GTAC 2 cut(s) 319, 661
RseI CAYNNNNRTG 1 cut(s) 84
SatI GCNGC 1 cut(s) 632
Sau3AI GATC 4 cut(s) 26, 75, 88, 672
Sau96I GGNCC 2 cut(s) 298, 381
SbfI CCTGCAGG 1 cut(s) 459
SchI GAGTC 3 cut(s) 113, 161, 403
ScrFI CCNGG 1 cut(s) 362
SdaI CCTGCAGG 1 cut(s) 459
SduI GDGCHC 4 cut(s) 21, 213, 361, 645
SetI ASST 9 cut(s) 84, 171, 261, 327, 438, 462, 474, 741, 779
SfaNI GCATC 3 cut(s) 473, 527, 721
SfcI CTRYAG 1 cut(s) 455
SgrAI CRCCGGYG 1 cut(s) 31
SinI GGWCC 2 cut(s) 298, 381
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse8387I CCTGCAGG 1 cut(s) 459
Sse9I AATT 2 cut(s) 351, 539
SsiI CCGC 5 cut(s) 216, 279, 288, 680, 755
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 2 cut(s) 165, 735
TaaI ACNGT 2 cut(s) 491, 640
TaiI ACGT 1 cut(s) 84
TaqI TCGA 3 cut(s) 306, 675, 702
TaqII GACCGA 1 cut(s) 164
TasI AATT 2 cut(s) 351, 539
TfiI GAWTC 2 cut(s) 95, 751
TscAI CASTG 1 cut(s) 507
TseI GCWGC 1 cut(s) 631
TspDTI ATGAA 1 cut(s) 497
TspRI CASTG 1 cut(s) 507
Tth111I GACNNNGTC 1 cut(s) 151
VpaK11BI GGWCC 2 cut(s) 298, 381
XapI RAATTY 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.