pycom02g15360

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001679
Physical Location & Seq
Reverse (-)
12168130 .. 12170037
1908 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15360.1

Sequence Viewer

Length: 1278 bp
ATGACTTCAAATGTGCCTGTCCCTTCCAATCTGCTCATACCTATCAAGAAATCCCTCTACCCTTATGGCTTGTCTGGTCCTTCTAATCCCACCTCAATAGCGTGGGGCGCTTACCATCTGGGATTTTCTGGCAACAACGATCCTGAACCTGGGAGGTGTCGCCGAACAGATGGGAAGAAATGGCGTTGCTCGAGAGATGCTGTTGCAGATCAGAAGTATTGTGAAAGGCACATCAACAGGGGACGCCATCGTTCAAGAAAGCCTGTGGAAGGCCAGCCTGGCCAGGCCGTCTCTGGGACCAGTAATTCAAAGAGGGTACCTATGAATTCGCCAATGTCAACGTCAGTGATGCCAAGCAGTGGTGCATCCAGCAGCCTCACAACCGCGCAGCAGCAATTCAAAAGCTTGCAGCCTGCTGTTGCTGCCAATCCTTCTGCAGATGCCTTTGTCAACAGGATGCAGGGGCAACAGGGAGTTTCCGTGATGTCTGCCCCTACCCTTAGGCTGAAATCCAACAATTCTACTTCTAACATTGGGAAACAAGAATTCCTAGTGGAAGAAGCCTCCCAAGCAGAGTTTGGACTTGTTTCTACTGATTCACTTCTCAACCCTTCACATAGGAACTCTTACATTTCTAAAGACTTTAGTGGTTCTTTCCTACACTTCGGTGATCAAGATACTCAAGACCATCATCCGCTTCGCCAGTTCATGGATGATTGGCCCAAAGACCAATCCTGTCACTCAGTCTCTACTTGGCCTGAAGAAGTAAAATCAGACTGGACTCAGCTATCAATGTCAATCCCTATGGCATCCTCAGAGTTCTCATCATCCTCGTCCTCGCCAAAGCATGAGAAACTTGCGCTCTCACCGCTGAGGCTATCCCGGGAGTTTGAGCCTGCCCAAATGAATTTGGGAATAAGCAAGGACATTAGCGAATCAAGCCAGAAGCAAACTAATTGGATACCTATCACTTGGGGAAATTCAATGGGGGGTCCCTTAGGAGAGGTCCTAACCAAAACCACCAGTGGTGGCGGCAAGATCTGCATTAATTCAAAATCCCCGCTTAGCCTCTTAAATGAAGGGTGGGATGGCAGCCCTCAGTTGGGATCTTCCCCAACTGGCGTCTTGCAGAAGTCAACTTTCTGTTCGCTTTCAAACAGCAGCTCAGGGAGCAGCCCGAGAGGAGGTGAGAACAAGAAGAGTAGTCTCGACGGGGCGAGCATTTATGATGACGTGCTAGGTTCAACCTTTGCAAGTTCCTCAGTTCCATCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QLQ WRC

Protein Analysis

426

Amino Acids

45.69

Weight (kDa)

8.8

Isoelectric Point (pI)

54.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRC PF08879 48 - 90 7.3e-21 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 316
AccB1I GGYRCC 1 cut(s) 316
AccB7I CCANNNNNTGG 2 cut(s) 359, 709
AccII CGCG 1 cut(s) 386
AciI CCGC 5 cut(s) 384, 695, 869, 1032, 1061
AclWI GGATC 2 cut(s) 134, 1114
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 4 cut(s) 325, 545, 907, 979
AcuI CTGAAG 1 cut(s) 780
AcyI GRCGYC 2 cut(s) 244, 1122
AfaI GTAC 1 cut(s) 318
AfiI CCNNNNNNNGG 8 cut(s) 149, 269, 294, 359, 709, 1102, 1103, 1184
AgsI TTSAA 8 cut(s) 9, 255, 309, 400, 984, 1053, 1155, 1245
AjiI CACGTC 1 cut(s) 1234
AjnI CCWGG 3 cut(s) 148, 277, 282
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 3 cut(s) 405, 787, 1164
AluI AGCT 3 cut(s) 405, 787, 1164
Alw26I GTCTC 3 cut(s) 295, 751, 1211
AlwI GGATC 2 cut(s) 134, 1114
Ama87I CYCGRG 3 cut(s) 190, 882, 1177
AoxI GGCC 5 cut(s) 271, 280, 285, 719, 755
ApeKI GCWGC 8 cut(s) 372, 388, 391, 409, 422, 1092, 1161, 1173
ApoI RAATTY 4 cut(s) 325, 545, 907, 979
AseI ATTAAT 1 cut(s) 1047
Asp718I GGTACC 1 cut(s) 316
AspLEI GCGC 3 cut(s) 110, 388, 862
AspS9I GGNCC 5 cut(s) 77, 297, 720, 992, 1006
AsuC2I CCSGG 2 cut(s) 883, 884
AsuHPI GGTGA 3 cut(s) 680, 858, 1199
AvaI CYCGRG 3 cut(s) 190, 882, 1177
AvaII GGWCC 4 cut(s) 77, 297, 992, 1006
AxyI CCTNAGG 2 cut(s) 500, 997
BalI TGGCCA 1 cut(s) 282
BanI GGYRCC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 872
BbvI GCAGC 8 cut(s) 384, 400, 403, 409, 421, 1104, 1173, 1185
BccI CCATC 6 cut(s) 123, 164, 255, 696, 1082, 1276
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 3 cut(s) 150, 279, 284
BciVI GTATCC 1 cut(s) 954
BclI TGATCA 1 cut(s) 670
BcnI CCSGG 2 cut(s) 883, 884
BcoDI GTCTC 3 cut(s) 295, 751, 1211
BfaI CTAG 2 cut(s) 551, 1238
BfmI CTRYAG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 111
BfuI GTATCC 1 cut(s) 954
BglI GCCNNNNNGGC 1 cut(s) 279
BglII AGATCT 1 cut(s) 1038
BisI GCNGC 9 cut(s) 373, 389, 392, 410, 423, 1033, 1093, 1162, 1174
BlpI GCTNAGC 1 cut(s) 1064
BlsI GCNGC 9 cut(s) 374, 390, 393, 411, 424, 1034, 1094, 1163, 1175
Bme1390I CCNGG 5 cut(s) 150, 279, 284, 883, 884
Bme18I GGWCC 4 cut(s) 77, 297, 992, 1006
BmeT110I CYCGRG 3 cut(s) 190, 882, 1177
BmgBI CACGTC 1 cut(s) 1234
BmgT120I GGNCC 5 cut(s) 77, 297, 720, 992, 1006
BmiI GGNNCC 4 cut(s) 298, 318, 993, 994
BmrFI CCNGG 5 cut(s) 150, 279, 284, 883, 884
BmsI GCATC 6 cut(s) 187, 339, 374, 430, 447, 818
Bpu10I CCTNAGC 2 cut(s) 872, 1165
Bpu1102I GCTNAGC 1 cut(s) 1064
BpuEI CTTGAG 1 cut(s) 666
BpuMI CCSGG 2 cut(s) 883, 884
BsaBI GATNNNNATC 1 cut(s) 965
BsaHI GRCGYC 2 cut(s) 244, 1122
BsaJI CCNNGG 2 cut(s) 149, 882
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bsc4I CCNNNNNNNGG 8 cut(s) 149, 269, 294, 359, 709, 1102, 1103, 1184
Bse1I ACTGG 5 cut(s) 300, 703, 782, 1023, 1123
Bse21I CCTNAGG 2 cut(s) 500, 997
Bse8I GATNNNNATC 1 cut(s) 965
BseBI CCWGG 3 cut(s) 150, 279, 284
BseDI CCNNGG 2 cut(s) 149, 882
BseGI GGATG 8 cut(s) 365, 462, 691, 718, 809, 827, 1093, 1268
BseJI GATNNNNATC 1 cut(s) 965
BseLI CCNNNNNNNGG 8 cut(s) 149, 269, 294, 359, 709, 1102, 1103, 1184
BseMII CTCAG 7 cut(s) 756, 797, 828, 863, 1112, 1179, 1275
BseNI ACTGG 5 cut(s) 300, 703, 782, 1023, 1123
BseRI GAGGAG 1 cut(s) 1197
BseXI GCAGC 8 cut(s) 384, 400, 403, 409, 421, 1104, 1173, 1185
Bsh1236I CGCG 1 cut(s) 386
BshFI GGCC 5 cut(s) 273, 282, 287, 721, 757
BshNI GGYRCC 1 cut(s) 316
BsiHKCI CYCGRG 3 cut(s) 190, 882, 1177
BsiSI CCGG 1 cut(s) 883
BslFI GGGAC 4 cut(s) 5, 255, 310, 978
BslI CCNNNNNNNGG 8 cut(s) 149, 269, 294, 359, 709, 1102, 1103, 1184
BsmAI GTCTC 3 cut(s) 295, 751, 1211
BsmBI CGTCTC 1 cut(s) 295
BsmFI GGGAC 4 cut(s) 5, 255, 310, 978
BsnI GGCC 5 cut(s) 273, 282, 287, 721, 757
BsoBI CYCGRG 3 cut(s) 190, 882, 1177
Bsp143I GATC 5 cut(s) 139, 208, 670, 1038, 1106
Bsp1720I GCTNAGC 1 cut(s) 1064
BspACI CCGC 5 cut(s) 384, 695, 869, 1032, 1061
BspANI GGCC 5 cut(s) 273, 282, 287, 721, 757
BspCNI CTCAG 7 cut(s) 755, 796, 827, 864, 1111, 1178, 1274
BspFNI CGCG 1 cut(s) 386
BspLI GGNNCC 4 cut(s) 298, 318, 993, 994
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 2 cut(s) 134, 1114
BspT107I GGYRCC 1 cut(s) 316
BsrI ACTGG 5 cut(s) 300, 703, 782, 1023, 1123
BssECI CCNNGG 2 cut(s) 149, 882
BssMI GATC 5 cut(s) 139, 208, 670, 1038, 1106
BssNI GRCGYC 2 cut(s) 244, 1122
Bst2UI CCWGG 3 cut(s) 150, 279, 284
Bst6I CTCTTC 1 cut(s) 1193
BstACI GRCGYC 2 cut(s) 244, 1122
BstAPI GCANNNNNTGC 1 cut(s) 1041
BstC8I GCNNGC 5 cut(s) 275, 407, 414, 897, 1219
BstENI CCTNNNNNAGG 1 cut(s) 267
BstF5I GGATG 8 cut(s) 365, 462, 691, 718, 809, 827, 1093, 1268
BstFNI CGCG 1 cut(s) 386
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 3 cut(s) 110, 388, 862
BstKTI GATC 5 cut(s) 142, 211, 673, 1041, 1109
BstMAI GTCTC 3 cut(s) 295, 751, 1211
BstMBI GATC 5 cut(s) 139, 208, 670, 1038, 1106
BstMWI GCNNNNNNNGC 8 cut(s) 107, 279, 422, 569, 868, 939, 1041, 1170
BstNI CCWGG 3 cut(s) 150, 279, 284
BstSCI CCNGG 5 cut(s) 148, 277, 282, 881, 882
BstSFI CTRYAG 1 cut(s) 435
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 8 cut(s) 384, 400, 403, 409, 421, 1104, 1173, 1185
BstX2I RGATCY 2 cut(s) 1038, 1106
BstYI RGATCY 2 cut(s) 1038, 1106
Bsu36I CCTNAGG 2 cut(s) 500, 997
BsuI GTATCC 1 cut(s) 954
BsuRI GGCC 5 cut(s) 273, 282, 287, 721, 757
BtrI CACGTC 1 cut(s) 1234
BtsCI GGATG 8 cut(s) 365, 462, 691, 718, 809, 827, 1093, 1268
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 3 cut(s) 351, 364, 1030
Cac8I GCNNGC 5 cut(s) 275, 407, 414, 897, 1219
CfoI GCGC 3 cut(s) 110, 388, 862
Cfr13I GGNCC 5 cut(s) 77, 297, 720, 992, 1006
Cfr9I CCCGGG 1 cut(s) 882
CseI GACGC 2 cut(s) 252, 1111
Csp6I GTAC 1 cut(s) 317
CviAII CATG 2 cut(s) 709, 848
CviQI GTAC 1 cut(s) 317
DpnI GATC 5 cut(s) 141, 210, 672, 1040, 1108
DpnII GATC 5 cut(s) 139, 208, 670, 1038, 1106
EaeI YGGCCR 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 1193
EarI CTCTTC 1 cut(s) 1193
Eco47I GGWCC 4 cut(s) 77, 297, 992, 1006
Eco57I CTGAAG 1 cut(s) 780
Eco81I CCTNAGG 2 cut(s) 500, 997
Eco88I CYCGRG 3 cut(s) 190, 882, 1177
EcoNI CCTNNNNNAGG 1 cut(s) 267
EcoO109I RGGNCCY 2 cut(s) 992, 1006
EcoRI GAATTC 2 cut(s) 325, 545
EcoRII CCWGG 3 cut(s) 148, 277, 282
Esp3I CGTCTC 1 cut(s) 295
FaeI CATG 2 cut(s) 712, 851
FaiI YATR 8 cut(s) 38, 66, 323, 618, 710, 806, 849, 1227
FaqI GGGAC 4 cut(s) 5, 255, 310, 978
FatI CATG 2 cut(s) 708, 847
FauI CCCGC 1 cut(s) 1068
FbaI TGATCA 1 cut(s) 670
Fnu4HI GCNGC 9 cut(s) 373, 389, 392, 410, 423, 1033, 1093, 1162, 1174
FokI GGATG 8 cut(s) 352, 469, 678, 725, 796, 814, 1100, 1255
Fsp4HI GCNGC 9 cut(s) 373, 389, 392, 410, 423, 1033, 1093, 1162, 1174
FspBI CTAG 2 cut(s) 551, 1238
GlaI GCGC 3 cut(s) 109, 387, 861
GluI GCNGC 9 cut(s) 373, 389, 392, 410, 423, 1033, 1093, 1162, 1174
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 5 cut(s) 273, 282, 287, 721, 757
HapII CCGG 1 cut(s) 883
HgaI GACGC 2 cut(s) 252, 1111
HhaI GCGC 3 cut(s) 110, 388, 862
Hin1I GRCGYC 2 cut(s) 244, 1122
Hin1II CATG 2 cut(s) 712, 851
Hin6I GCGC 3 cut(s) 108, 386, 860
HinP1I GCGC 3 cut(s) 108, 386, 860
HincII GTYRAC 3 cut(s) 339, 451, 1137
HindII GTYRAC 3 cut(s) 339, 451, 1137
HindIII AAGCTT 1 cut(s) 403
HinfI GANTC 3 cut(s) 596, 781, 935
HpaII CCGG 1 cut(s) 883
HphI GGTGA 3 cut(s) 680, 858, 1199
Hpy166II GTNNAC 3 cut(s) 339, 451, 1137
Hpy188I TCNGA 3 cut(s) 213, 775, 817
Hpy188III TCNNGA 7 cut(s) 46, 143, 192, 255, 674, 683, 1208
Hpy8I GTNNAC 3 cut(s) 339, 451, 1137
Hpy99I CGWCG 1 cut(s) 1214
HpyAV CCTTC 6 cut(s) 33, 90, 263, 441, 621, 1073
HpyCH4IV ACGT 2 cut(s) 341, 1233
HpyCH4V TGCA 8 cut(s) 206, 365, 409, 437, 460, 1044, 1129, 1253
HpyF10VI GCNNNNNNNGC 8 cut(s) 107, 279, 422, 569, 868, 939, 1041, 1170
HpySE526I ACGT 2 cut(s) 341, 1233
Hsp92I GRCGYC 2 cut(s) 244, 1122
Hsp92II CATG 2 cut(s) 712, 851
HspAI GCGC 3 cut(s) 108, 386, 860
KflI GGGWCCC 1 cut(s) 992
KpnI GGTACC 1 cut(s) 320
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 5 cut(s) 139, 208, 670, 1038, 1106
LmnI GCTCC 1 cut(s) 1170
Lsp1109I GCAGC 8 cut(s) 384, 400, 403, 409, 421, 1104, 1173, 1185
LweI GCATC 6 cut(s) 187, 339, 374, 430, 447, 818
MaeI CTAG 2 cut(s) 551, 1238
MaeII ACGT 2 cut(s) 341, 1233
MaeIII GTNAC 1 cut(s) 737
MalI GATC 5 cut(s) 141, 210, 672, 1040, 1108
MboI GATC 5 cut(s) 139, 208, 670, 1038, 1106
MboII GAAGA 5 cut(s) 187, 569, 773, 1101, 1210
MflI RGATCY 2 cut(s) 1038, 1106
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 9 cut(s) 304, 325, 395, 517, 545, 907, 955, 979, 1048
MluNI TGGCCA 1 cut(s) 282
MlyI GAGTC 1 cut(s) 775
MmeI TCCRAC 1 cut(s) 537
Mox20I TGGCCA 1 cut(s) 282
MscI TGGCCA 1 cut(s) 282
MseI TTAA 2 cut(s) 1047, 1073
MslI CAYNNNNRTG 1 cut(s) 666
Msp20I TGGCCA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 871
MspI CCGG 1 cut(s) 883
MspR9I CCNGG 5 cut(s) 150, 279, 284, 883, 884
MvaI CCWGG 3 cut(s) 150, 279, 284
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 8 cut(s) 107, 279, 422, 569, 868, 939, 1041, 1170
NciI CCSGG 2 cut(s) 883, 884
NdeII GATC 5 cut(s) 139, 208, 670, 1038, 1106
NlaIII CATG 2 cut(s) 712, 851
NlaIV GGNNCC 4 cut(s) 298, 318, 993, 994
NmuCI GTSAC 1 cut(s) 737
OliI CACNNNNGTG 1 cut(s) 666
PaeR7I CTCGAG 1 cut(s) 190
PfeI GAWTC 2 cut(s) 596, 935
PflMI CCANNNNNTGG 2 cut(s) 359, 709
PkrI GCNGC 9 cut(s) 374, 390, 393, 411, 424, 1034, 1094, 1163, 1175
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
PpuMI RGGWCCY 2 cut(s) 992, 1006
PshBI ATTAAT 1 cut(s) 1047
Psp5II RGGWCCY 2 cut(s) 992, 1006
Psp6I CCWGG 3 cut(s) 148, 277, 282
PspGI CCWGG 3 cut(s) 148, 277, 282
PspN4I GGNNCC 4 cut(s) 298, 318, 993, 994
PspPI GGNCC 5 cut(s) 77, 297, 720, 992, 1006
PspPPI RGGWCCY 2 cut(s) 992, 1006
PstI CTGCAG 1 cut(s) 439
PsuI RGATCY 2 cut(s) 1038, 1106
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 666
SaqAI TTAA 2 cut(s) 1047, 1073
SatI GCNGC 9 cut(s) 373, 389, 392, 410, 423, 1033, 1093, 1162, 1174
Sau3AI GATC 5 cut(s) 139, 208, 670, 1038, 1106
Sau96I GGNCC 5 cut(s) 77, 297, 720, 992, 1006
SchI GAGTC 1 cut(s) 775
ScrFI CCNGG 5 cut(s) 150, 279, 284, 883, 884
SfaNI GCATC 6 cut(s) 187, 339, 374, 430, 447, 818
SfcI CTRYAG 1 cut(s) 435
SfiI GGCCNNNNNGGCC 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 190
SinI GGWCC 4 cut(s) 77, 297, 992, 1006
SlaI CTCGAG 1 cut(s) 190
SmaI CCCGGG 1 cut(s) 884
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 2 cut(s) 190, 681
SmoI CTYRAG 2 cut(s) 190, 681
Sse9I AATT 9 cut(s) 304, 325, 395, 517, 545, 907, 955, 979, 1048
SsiI CCGC 5 cut(s) 384, 695, 869, 1032, 1061
SspMI CTAG 2 cut(s) 551, 1238
StyD4I CCNGG 5 cut(s) 148, 277, 282, 881, 882
TaiI ACGT 2 cut(s) 344, 1236
TaqI TCGA 2 cut(s) 191, 1209
TasI AATT 9 cut(s) 304, 325, 395, 517, 545, 907, 955, 979, 1048
TauI GCSGC 1 cut(s) 1035
TfiI GAWTC 2 cut(s) 596, 935
Tru1I TTAA 2 cut(s) 1047, 1073
Tru9I TTAA 2 cut(s) 1047, 1073
TscAI CASTG 3 cut(s) 351, 364, 1030
TseFI GTSAC 1 cut(s) 737
TseI GCWGC 8 cut(s) 372, 388, 391, 409, 422, 1092, 1161, 1173
Tsp45I GTSAC 1 cut(s) 737
TspDTI ATGAA 4 cut(s) 338, 697, 920, 1092
TspGWI ACGGA 1 cut(s) 469
TspMI CCCGGG 1 cut(s) 882
TspRI CASTG 3 cut(s) 351, 364, 1030
Van91I CCANNNNNTGG 2 cut(s) 359, 709
VpaK11BI GGWCC 4 cut(s) 77, 297, 992, 1006
VspI ATTAAT 1 cut(s) 1047
XagI CCTNNNNNAGG 1 cut(s) 267
XapI RAATTY 4 cut(s) 325, 545, 907, 979
XcmI CCANNNNNNNNNTGG 2 cut(s) 290, 575
XhoI CTCGAG 1 cut(s) 190
XmaI CCCGGG 1 cut(s) 882
XspI CTAG 2 cut(s) 551, 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.