pycom03g00120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002354
Physical Location & Seq
Reverse (-)
134410 .. 138224
3815 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1107 bp
ATGGGCCTGGTGGTTGCAAAACGTGCGAGATTTGACCCAAAAAAAAAAGAGGCGGCCATAACTGGGCAATTGGTTCTCCATCCAAATTTTGATTTCTTAATTTCCGACAAAACCACAAAATTCCTCAGCCTCATCCCTCATCCGGTCATCCCCCATCCCCCAGAACCAAAGAAGCAAAGAAGCAAAGAAGCAAAGAAGCAAAGAAGCAAAGAAGCAAAGAAGCAAAGAAGCAAAGAAGCAACGAAGATGAAGGCAGCTCACCCACTGGTGCTAGTTTTTGTGGGTTTGCTTGCGCTTGCAGTAGCACCTTCCACTTCAGCTTCCACAGTGCCAGCTTTCCTCTGGTCCCCTCATTATCATCAAGTCAAGGCAGCCGTAAATTATCAGACAATATCCCCCAAGGATTTAGCAAAGTCTCTTCTTTCCGAAGGTGGCTGGTCCAACGTACTGTGTTCCGCAAATAAAGTTCATCAGCCTTTGGAGCTGGCTCTTGTCTTCGTGGGTGGAGAGTTGCAATCTTCAGATATTCCTGCAAACAGACATGCAGATCCAGCTCTTGTGGACTTCCTCAAAACCTCTTTCTCAGGATCCAATTTCTCCATGGCCTTCCCTTTTGTTGCTGCACCGGAGGAGGATTCTATGGAAAATTCTTTGGTGTTAGAGATTTCAGAGACTTGTGGGCAGGATTTGGGATTCAGTAATGTTACTTTATTTGGGTCATGCTCTATTGAGGGTGAAAATTTTCAAAAGCTCGCAAATGTGCAATCATTCCATGATTATCTTGTTTCAGGGGTGGAGAAGAGATCCAAAGGGGAAGTAGATTTGGTTGTGTTCTGCCATAAACGATCTGAATCTTCCAAAGAACTTGACCAAACACATTCTGAGGGTAAAGTTTTTTCTGACCTTGTTAATTCCATGGATCGATCGTTAATGCAAAGAAATGCATCAGCCAATTCGACGGCCTGTGATGAAGTTTGTCAGATAAAATCATCTCTTCTAGAGGGACTTCTAGTTGGAATCGTTTTGCTTATAATCTTAATATCGGGCATTTGCTGTATGATGGGGATTGACACTCCGACGAGATTTGAGTTGCCCCAAGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

369

Amino Acids

40.2

Weight (kDa)

7.16

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ac45-VOA1_TM PF20520 332 - 363 1.1e-08 V0 complex accessory subunit Ac45/VOA1 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1031
AciI CCGC 2 cut(s) 53, 456
AclWI GGATC 5 cut(s) 542, 582, 595, 798, 927
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 4 cut(s) 85, 119, 646, 739
AcuI CTGAAG 2 cut(s) 300, 504
AfaI GTAC 1 cut(s) 447
AfiI CCNNNNNNNGG 2 cut(s) 63, 142
AgsI TTSAA 1 cut(s) 746
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 6 cut(s) 257, 320, 335, 484, 554, 751
AluI AGCT 6 cut(s) 257, 320, 335, 484, 554, 751
Alw26I GTCTC 2 cut(s) 420, 665
AlwI GGATC 5 cut(s) 542, 582, 595, 798, 927
AoxI GGCC 4 cut(s) 4, 54, 603, 960
ApeKI GCWGC 3 cut(s) 254, 371, 620
ApoI RAATTY 4 cut(s) 85, 119, 646, 739
Asp700I GAANNNNTTC 1 cut(s) 741
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 3 cut(s) 4, 345, 438
AsuHPI GGTGA 2 cut(s) 251, 746
AvaII GGWCC 2 cut(s) 345, 438
BamHI GGATCC 1 cut(s) 587
BbsI GAAGAC 1 cut(s) 487
BbvCI CCTCAGC 1 cut(s) 125
BbvI GCAGC 3 cut(s) 266, 383, 607
BccI CCATC 3 cut(s) 87, 162, 1054
BceAI ACGGC 2 cut(s) 359, 975
BciT130I CCWGG 1 cut(s) 8
BcoDI GTCTC 2 cut(s) 420, 665
BfaI CTAG 3 cut(s) 272, 998, 1010
BisI GCNGC 4 cut(s) 54, 255, 372, 621
BlsI GCNGC 4 cut(s) 55, 256, 373, 622
Bme1390I CCNGG 1 cut(s) 8
Bme18I GGWCC 2 cut(s) 345, 438
BmgT120I GGNCC 3 cut(s) 4, 345, 438
BmiI GGNNCC 2 cut(s) 347, 589
BmrFI CCNGG 1 cut(s) 8
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 953
BmuI ACTGGG 1 cut(s) 72
BpiI GAAGAC 1 cut(s) 487
Bpu10I CCTNAGC 1 cut(s) 125
Bsa29I ATCGAT 1 cut(s) 922
BsaBI GATNNNNATC 1 cut(s) 850
BsaJI CCNNGG 3 cut(s) 399, 600, 915
BsaWI WCCGGW 2 cut(s) 142, 625
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 142
Bse1I ACTGG 2 cut(s) 67, 270
Bse8I GATNNNNATC 1 cut(s) 850
BseBI CCWGG 1 cut(s) 8
BseCI ATCGAT 1 cut(s) 922
BseDI CCNNGG 3 cut(s) 399, 600, 915
BseGI GGATG 5 cut(s) 79, 132, 139, 147, 154
BseJI GATNNNNATC 1 cut(s) 850
BseLI CCNNNNNNNGG 2 cut(s) 63, 142
BseMII CTCAG 3 cut(s) 139, 597, 873
BseNI ACTGG 2 cut(s) 67, 270
BseRI GAGGAG 1 cut(s) 644
BseXI GCAGC 3 cut(s) 266, 383, 607
BsgI GTGCAG 1 cut(s) 606
Bsh1285I CGRYCG 1 cut(s) 926
BshFI GGCC 4 cut(s) 6, 56, 605, 962
BshVI ATCGAT 1 cut(s) 922
BsiEI CGRYCG 1 cut(s) 926
BsiSI CCGG 2 cut(s) 143, 626
BslFI GGGAC 2 cut(s) 331, 1017
BslI CCNNNNNNNGG 2 cut(s) 63, 142
BsmAI GTCTC 2 cut(s) 420, 665
BsmFI GGGAC 2 cut(s) 331, 1017
BsnI GGCC 4 cut(s) 6, 56, 605, 962
Bsp143I GATC 6 cut(s) 547, 587, 803, 845, 919, 923
Bsp19I CCATGG 2 cut(s) 600, 915
BspACI CCGC 2 cut(s) 53, 456
BspANI GGCC 4 cut(s) 6, 56, 605, 962
BspCNI CTCAG 3 cut(s) 138, 596, 874
BspDI ATCGAT 1 cut(s) 922
BspLI GGNNCC 2 cut(s) 347, 589
BspPI GGATC 5 cut(s) 542, 582, 595, 798, 927
BsrI ACTGG 2 cut(s) 67, 270
BssECI CCNNGG 3 cut(s) 399, 600, 915
BssMI GATC 6 cut(s) 547, 587, 803, 845, 919, 923
BssT1I CCWWGG 3 cut(s) 399, 600, 915
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 2 cut(s) 328, 450
Bst6I CTCTTC 3 cut(s) 423, 794, 999
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 5 cut(s) 291, 297, 333, 486, 753
BstDEI CTNAG 3 cut(s) 125, 583, 882
BstDSI CCRYGG 2 cut(s) 600, 915
BstF5I GGATG 5 cut(s) 79, 132, 139, 147, 154
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 6 cut(s) 550, 590, 806, 848, 922, 926
BstMAI GTCTC 2 cut(s) 420, 665
BstMBI GATC 6 cut(s) 547, 587, 803, 845, 919, 923
BstMCI CGRYCG 1 cut(s) 926
BstMWI GCNNNNNNNGC 3 cut(s) 23, 481, 551
BstNI CCWGG 1 cut(s) 8
BstNSI RCATGY 1 cut(s) 545
BstSCI CCNGG 1 cut(s) 6
BstV1I GCAGC 3 cut(s) 266, 383, 607
BstV2I GAAGAC 1 cut(s) 487
BstX2I RGATCY 3 cut(s) 547, 587, 803
BstYI RGATCY 3 cut(s) 547, 587, 803
Bsu15I ATCGAT 1 cut(s) 922
BsuRI GGCC 4 cut(s) 6, 56, 605, 962
BsuTUI ATCGAT 1 cut(s) 922
BtgI CCRYGG 2 cut(s) 600, 915
BtsCI GGATG 5 cut(s) 79, 132, 139, 147, 154
BtsIMutI CAGTG 2 cut(s) 263, 333
Cac8I GCNNGC 5 cut(s) 291, 297, 333, 486, 753
CfoI GCGC 1 cut(s) 295
Cfr13I GGNCC 3 cut(s) 4, 345, 438
ClaI ATCGAT 1 cut(s) 922
Csp6I GTAC 1 cut(s) 446
CviAII CATG 5 cut(s) 542, 601, 720, 773, 916
CviQI GTAC 1 cut(s) 446
DdeI CTNAG 3 cut(s) 125, 583, 882
DpnI GATC 6 cut(s) 549, 589, 805, 847, 921, 925
DpnII GATC 6 cut(s) 547, 587, 803, 845, 919, 923
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 3 cut(s) 423, 794, 999
EarI CTCTTC 3 cut(s) 423, 794, 999
Eco130I CCWWGG 3 cut(s) 399, 600, 915
Eco47I GGWCC 2 cut(s) 345, 438
Eco57I CTGAAG 2 cut(s) 300, 504
EcoRII CCWGG 1 cut(s) 6
EcoT14I CCWWGG 3 cut(s) 399, 600, 915
EcoT22I ATGCAT 1 cut(s) 946
ErhI CCWWGG 3 cut(s) 399, 600, 915
FaeI CATG 5 cut(s) 545, 604, 723, 776, 919
FaqI GGGAC 2 cut(s) 331, 1017
FatI CATG 5 cut(s) 541, 600, 719, 772, 915
Fnu4HI GCNGC 4 cut(s) 54, 255, 372, 621
FokI GGATG 5 cut(s) 66, 119, 126, 134, 141
Fsp4HI GCNGC 4 cut(s) 54, 255, 372, 621
FspBI CTAG 3 cut(s) 272, 998, 1010
GlaI GCGC 1 cut(s) 294
GluI GCNGC 4 cut(s) 54, 255, 372, 621
HaeIII GGCC 4 cut(s) 6, 56, 605, 962
HapII CCGG 2 cut(s) 143, 626
HhaI GCGC 1 cut(s) 295
Hin1II CATG 5 cut(s) 545, 604, 723, 776, 919
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HinfI GANTC 5 cut(s) 635, 693, 851, 1017, 1100
HpaII CCGG 2 cut(s) 143, 626
HphI GGTGA 2 cut(s) 251, 746
Hpy166II GTNNAC 1 cut(s) 562
Hpy188III TCNNGA 3 cut(s) 585, 998, 1104
Hpy8I GTNNAC 1 cut(s) 562
Hpy99I CGWCG 2 cut(s) 961, 1081
HpyAV CCTTC 4 cut(s) 244, 318, 422, 616
HpyCH4III ACNGT 2 cut(s) 328, 450
HpyCH4IV ACGT 2 cut(s) 22, 444
HpyCH4V TGCA 9 cut(s) 17, 299, 514, 533, 545, 623, 763, 934, 944
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 481, 551
HpyF3I CTNAG 3 cut(s) 125, 583, 882
HpySE526I ACGT 2 cut(s) 22, 444
Hsp92II CATG 5 cut(s) 545, 604, 723, 776, 919
HspAI GCGC 1 cut(s) 293
Kzo9I GATC 6 cut(s) 547, 587, 803, 845, 919, 923
LmnI GCTCC 1 cut(s) 481
Lsp1109I GCAGC 3 cut(s) 266, 383, 607
LweI GCATC 1 cut(s) 953
MaeI CTAG 3 cut(s) 272, 998, 1010
MaeII ACGT 2 cut(s) 22, 444
MaeIII GTNAC 1 cut(s) 703
MalI GATC 6 cut(s) 549, 589, 805, 847, 921, 925
MboI GATC 6 cut(s) 547, 587, 803, 845, 919, 923
MboII GAAGA 7 cut(s) 256, 410, 487, 510, 811, 846, 986
MfeI CAATTG 1 cut(s) 68
MflI RGATCY 3 cut(s) 547, 587, 803
MmeI TCCRAC 4 cut(s) 129, 465, 994, 1100
Mph1103I ATGCAT 1 cut(s) 946
MroXI GAANNNNTTC 1 cut(s) 741
MseI TTAA 4 cut(s) 98, 909, 929, 1037
MspI CCGG 2 cut(s) 143, 626
MspR9I CCNGG 1 cut(s) 8
MunI CAATTG 1 cut(s) 68
MvaI CCWGG 1 cut(s) 8
MwoI GCNNNNNNNGC 3 cut(s) 23, 481, 551
NcoI CCATGG 2 cut(s) 600, 915
NdeII GATC 6 cut(s) 547, 587, 803, 845, 919, 923
NlaIII CATG 5 cut(s) 545, 604, 723, 776, 919
NlaIV GGNNCC 2 cut(s) 347, 589
NsiI ATGCAT 1 cut(s) 946
NspI RCATGY 1 cut(s) 545
PdmI GAANNNNTTC 1 cut(s) 741
PfeI GAWTC 4 cut(s) 635, 693, 851, 1017
PkrI GCNGC 4 cut(s) 55, 256, 373, 622
Ple19I CGATCG 1 cut(s) 926
PsiI TTATAA 1 cut(s) 1031
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 2 cut(s) 347, 589
PspPI GGNCC 3 cut(s) 4, 345, 438
PsuI RGATCY 3 cut(s) 547, 587, 803
PvuI CGATCG 1 cut(s) 926
RsaI GTAC 1 cut(s) 447
RsaNI GTAC 1 cut(s) 446
SaqAI TTAA 4 cut(s) 98, 909, 929, 1037
SatI GCNGC 4 cut(s) 54, 255, 372, 621
Sau3AI GATC 6 cut(s) 547, 587, 803, 845, 919, 923
Sau96I GGNCC 3 cut(s) 4, 345, 438
ScrFI CCNGG 1 cut(s) 8
SfaNI GCATC 1 cut(s) 953
SinI GGWCC 2 cut(s) 345, 438
SsiI CCGC 2 cut(s) 53, 456
SspMI CTAG 3 cut(s) 272, 998, 1010
StyD4I CCNGG 1 cut(s) 6
StyI CCWWGG 3 cut(s) 399, 600, 915
TaaI ACNGT 2 cut(s) 328, 450
TaiI ACGT 2 cut(s) 25, 447
TaqI TCGA 2 cut(s) 922, 956
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 4 cut(s) 635, 693, 851, 1017
Tru1I TTAA 4 cut(s) 98, 909, 929, 1037
Tru9I TTAA 4 cut(s) 98, 909, 929, 1037
TscAI CASTG 2 cut(s) 270, 333
TseI GCWGC 3 cut(s) 254, 371, 620
TspDTI ATGAA 3 cut(s) 263, 458, 984
TspRI CASTG 2 cut(s) 270, 333
VpaK11BI GGWCC 2 cut(s) 345, 438
XapI RAATTY 4 cut(s) 85, 119, 646, 739
XbaI TCTAGA 1 cut(s) 997
XceI RCATGY 1 cut(s) 545
XcmI CCANNNNNNNNNTGG 2 cut(s) 339, 598
XmnI GAANNNNTTC 1 cut(s) 741
XspI CTAG 3 cut(s) 272, 998, 1010
Zsp2I ATGCAT 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.