pycom03g09430

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001971
Physical Location & Seq
Forward (+)
7983590 .. 7995727
12138 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3639 bp
ATGAGCAAGTCTTTAGTAGACTATGGAGAGCAAAAGTCATGTGCAGTGACCACTGCAAGACGTCTTGCTGATTTTCTTGGTGATACGATGGTCAAAGAGAAAGGACTGCGTTGCCAGTATATAGTTGCATGTGAACCAAAGGGCACACCTGTTAGTGAGCGTGCTGTTCCAGTTGCAATATTTGAAACAGATGCTGAAATTATGAAGTTTTATGTACGGAGGTGGTGCAAGGTTTCATCAGATGTTGGCATTCGATCCATTATTGACTGGACCTATTACAAACAACGCCTTGGTTCAGCAATTCAGAAAATTGTCACTATTCCGGCTGCAATGCAAAAGGTTGCAAATCCAGTTCCTAGAGTAGCTCATCCTGATTGGCTGCATAAGAAGGTTCGTGAGAAGGAGGACAAATTTCGCCAGCGCAAGTTGGTTGATATCTTCAAGTCATTGAACAGGGATCATATGCTAGAAAAGAGCAGTGATGCTATTGACTCTAGTCATGTGCTTGATCAAGAGATTGTTGATGATTTGGAAGACTTCAACAAAAAAAACAGTTCCATAAATGGACCCAGACCAATTGTTCGCTGTTATGAAGTCAATCGCAAGAAAAATTCAGTTGAAACAACTTGCCAAATAGATTCACAGCAACAACAAACTAGTAATAACGAAAATGAGCGTCAAGAGCAAAATGGTGTCCCTTTACAGTGCATTGATAGAAATATGGATTATCATGGTTGGCTTGAAACGAAGAAGAGAAAATGGAAAGATACTCTTGACAGGAGGAAAAAGCAAAGATTGAATGATGCAAGGTCTGCTCACCATGATAATAATGAGCTGCTTGGTGGTGGTAGAAATAAGAAAGGAACTCAGGGAAAAATAGGTGTGAATTCATATTTTAGGAGGCATGAAGAAGCCCTAACACGCTGTCATTGGCAGATATTACAACTAGTGCCCGGTTCTCAGGGTGACCAATTTTACGCTTGGGTGGTTATTGAAGGAATCATGCTTAAGATCCCTGTTACTGTTCCAAGAGTTTTTTACATTAATTCTAAAGCACCTATTGAAAAAATATTTAATAAAATATCTGAAGAAATACCTCCAGTAAAGGATGTCAAAAAGGCTCTTCCTCATGGAAGGCATATCTACAACCTATATGAGGCTACAATTGATGAGGGTCAGTTTAGGACAATTAGAAAAAAGCTTGCAGCTCTTCTTGGCGATCCAGAAGTTGAGGGAATATATGAAACAAGGGTACCCTTGGAGTTCAATGCAATCATTCAGATTGGTTGTGTTTGCAAAGTGGATAAAACTGCTAAGAAAAGAAATGTCCAAGATGGTTGGAGTGTGAGTGAACTGCACATGAAGACAACTACAGAGTGCTCATATCTGGATCAATCCATCTCTTTCTTTTACCTGTATCAGAGCACATCTGAAGGGCGAGCTATATATATTGCCTATTTTCCTGCATCAACAACAATAAAGGTTGTGGTGGTAAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTGGAAAAATTATTTCGTGATGCTTGCCAAGCAGTATCAGTTCAGCCACCTCCTAGGAATGGTATCATTATAAAGGTGGACTATGTTGGATTCGTCAAGGATGCGGAAACAGTTTTGCACCGTTCAATTAATGAGTACAGACATGAATATCCTGGACCTACAATTGCTGTTATCGAATGCCCAAATTTTCAAATGATGAAGTCAGGTATAGGAGCTCTTGATGATTTTCCGTGTGTGAGCATTCCCTCTAATGCTCGTGATAGTCTCTATCAGGTTCTTGGGTGGCAGCAAGTGGCTGCAAAAATTGGAATGCAGCGTTGTGCTGCATCACCTCAGTGGTTGAATGAGAGAATTTCACTCTCAAGATATGCCCATGTTCCATTGGGAAATTTCGAAGTTGACTGGCTTTTACATACAGCAGATATCTTCTTTTCAAGAGCACTACGTGATCAACAACATGTGCTTTGGATGTCTGATGATGGTATTCCAGATCTAGGAGGCATTAATGAAGAAGATTCATGTTTTGCTGATGAGGTCTGTGAACTTAATTTTTCTTACCCTGGAGCATATAGGAAGGTCACTGTGGAGCTAAAGATACATCATCTAGCTGTAAATGCTCTTTTAAAAAGCAACCAGGTGAATGAAATGGAAGGAGGTTCTTTGCTAGGATTTGAATCAGACGTGAACTCCAGAGTACATACTTTTGATGCACATGTTGGCTTTGATGAAGCTACCTCATGTGCTCCTACATTCCGTGTCCTGAAACAGTTGATTCAGAGGTGTCTTTCAAATGCAGTTACATCTGGAAATGTGTACGCTGATGCACTGTTGCAACATTTATATCGATGGCTTTCCAGCCCCCAGTCTAAGCTTCATGATCCATCTCTTCACCGCATACTTCATAAGGTCATGAAAAAGGTGCTTGCAATTTTAATGACTGAGTTCCGCAAATTGGGTGCCAAGATCATGTTCGCAAATTTCTACAAGGTTATTATAGACACAGGAAAACTTGAGATCGATCTTTCAGCTGCCAAAGCGTATTGTGATAGCTTGCTTAAAACTCTGCAAACGAGAGAACTATTTGAGTGGATAGAGCTTGAACCACTACACTTCTGGCATTCTTTGCTTTTCATGGATCAGTTTAACTATGGTGGAATTCCGGTTAGAGCTGAAGGAAGCATGCATGGTGAAGCTCAATTGGATATTGTATCAAGCTGGAATATTGCTGAATACTTGCCCAAGAAAATTCAGGACCACTTTGTTTATATTGTCTCTCAGTATCTGTATATTCCCTGGGATTATGCACAGAAGCAGGCAGCAGTTAGAACATCTTTACAGAGTGATAGCATTTGCACGCCATCAATCACTGTAGCAGATGCTGAGACTTTTGAGTCTCATATGATAGATTTCCTTAAAGGACAGATTGGCTCATATTTTACAGATAAACTTCTGGGAATTGTTCATGATACCATGCTTCATATGAAGGAACTGAAAAAATCAGAGAATGACCGTGATACATCTCTTGAGCTGCCACAAATTGCTGGTAATATCCACCGAGGGGATGCAGCACTGGAGTTCATCAAGCACGTCTGCGGTGTTTTCTCCATTGACCAAAATGTTGTCCATGATGTTTTGGTAATGAGAAAGAATCTGTTGAAGATTGTACGTGTGAGGGAGTTTGCCCCCGAAGCAGAGTTTCATGATCCCGGCCCAACCTTCACTTTACCAAATGTCATTTGCAGCTACTGCAACGATTGTAGAGACCTGGACTTGTGCCGTGACTCAGCTCTTCTAGCTGGGGAATGGCGTTGCTGTGTACCACAATGCGGGCAGCTTTATGACAAAGAGGTAATGGAGAACGCCCTTCTTCAGATTGTGCGGCAGAGAGAGCGTCTTTACCACCTACAAGATCTCGAATGCCTCAGGTGTCACCAAGTGAAAGCTGCACGCTTAGCAGAGCAATGTGTATGCTCTGGCTCATATAAGTGCAGGCAAAACGCAACTGAATTTCGTACCCAGATGGAGTTAATTTGGAAGATTGCCATTCATCAGAAATTTCAACTTCTCCAAGAGTGCACTGAATGGATTTTAGAAGTAAATAACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

1213

Amino Acids

138.74

Weight (kDa)

7.08

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
POL2_thumb PF22634 1 - 112 1.2e-44 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 530 - 896 4.9e-114 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 1073 - 1139 2.8e-19 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 1145 - 1201 1.7e-13 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1601
AasI GACNNNNNNGTC 1 cut(s) 2920
AatII GACGTC 1 cut(s) 64
Acc65I GGTACC 1 cut(s) 1252
AccB1I GGYRCC 2 cut(s) 1252, 2486
AccI GTMKAC 1 cut(s) 18
AciI CCGC 6 cut(s) 1634, 2422, 2476, 3123, 3357, 3409
AclWI GGATC 8 cut(s) 249, 465, 1006, 1214, 1398, 2402, 2673, 3227
AcuI CTGAAG 4 cut(s) 1107, 1452, 2721, 3383
AcyI GRCGYC 1 cut(s) 61
AdeI CACNNNGTG 2 cut(s) 1976, 3466
AfaI GTAC 8 cut(s) 216, 1254, 1667, 2226, 2345, 3195, 3348, 3544
AfiI CCNNNNNNNGG 6 cut(s) 1156, 1589, 2024, 2482, 3088, 3356
AflII CTTAAG 1 cut(s) 1007
AflIII ACRYGT 3 cut(s) 1987, 2242, 3196
AhlI ACTAGT 2 cut(s) 656, 946
AjiI CACGTC 2 cut(s) 2212, 3118
AjnI CCWGG 5 cut(s) 1681, 2089, 2163, 2822, 3294
AjuI GAANNNNNNNTTGG 2 cut(s) 2483, 2515
AleI CACNNNNGTG 1 cut(s) 1864
Alw21I GWGCWC 6 cut(s) 1382, 1427, 1747, 1972, 2275, 3608
Alw26I GTCTC 5 cut(s) 1799, 2806, 2906, 2928, 3285
Alw44I GTGCAC 1 cut(s) 3604
AlwI GGATC 8 cut(s) 249, 465, 1006, 1214, 1398, 2402, 2673, 3227
AlwNI CAGNNNCTG 4 cut(s) 194, 2812, 2909, 3276
AoxI GGCC 1 cut(s) 3238
ApaLI GTGCAC 1 cut(s) 3604
ArsI GACNNNNNNTTYG 2 cut(s) 564, 596
AseI ATTAAT 3 cut(s) 1044, 1659, 2034
Asp718I GGTACC 1 cut(s) 1252
AspA2I CCTAGG 1 cut(s) 1583
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 5 cut(s) 270, 566, 1685, 2782, 3239
AsuC2I CCSGG 2 cut(s) 954, 3237
AsuHPI GGTGA 9 cut(s) 92, 809, 977, 1512, 1851, 2179, 2411, 2729, 3452
AsuII TTCGAA 1 cut(s) 1923
AvaII GGWCC 4 cut(s) 270, 566, 1685, 2782
AvrII CCTAGG 1 cut(s) 1583
AxyI CCTNAGG 1 cut(s) 3452
BaeGI GKGCMC 3 cut(s) 146, 954, 3608
BaeI ACNNNNGTAYC 4 cut(s) 75, 108, 1236, 1269
BanI GGYRCC 2 cut(s) 1252, 2486
BanII GRGCYC 1 cut(s) 1747
BauI CACGAG 1 cut(s) 1785
BbsI GAAGAC 2 cut(s) 540, 1370
Bbv12I GWGCWC 6 cut(s) 1382, 1427, 1747, 1972, 2275, 3608
BccI CCATC 9 cut(s) 82, 1328, 1406, 1531, 2003, 2370, 2419, 2896, 3544
BceAI ACGGC 1 cut(s) 3291
BciT130I CCWGG 5 cut(s) 1683, 2091, 2165, 2824, 3296
BclI TGATCA 2 cut(s) 508, 1978
BcnI CCSGG 2 cut(s) 954, 3237
BcoDI GTCTC 5 cut(s) 1799, 2806, 2906, 2928, 3285
BcuI ACTAGT 2 cut(s) 656, 946
BfmI CTRYAG 2 cut(s) 1371, 2898
BfrI CTTAAG 1 cut(s) 1007
BglII AGATCT 2 cut(s) 2020, 3439
BlnI CCTAGG 1 cut(s) 1583
BlpI GCTNAGC 1 cut(s) 3481
Bme1390I CCNGG 7 cut(s) 954, 1683, 2091, 2165, 2824, 3237, 3296
Bme18I GGWCC 4 cut(s) 270, 566, 1685, 2782
BmgBI CACGTC 2 cut(s) 2212, 3118
BmgT120I GGNCC 5 cut(s) 270, 566, 1685, 2782, 3239
BmiI GGNNCC 3 cut(s) 568, 1254, 2488
BmrFI CCNGG 7 cut(s) 954, 1683, 2091, 2165, 2824, 3237, 3296
BmrI ACTGGG 1 cut(s) 2386
BmuI ACTGGG 1 cut(s) 2386
BoxI GACNNNNGTC 1 cut(s) 495
BpiI GAAGAC 2 cut(s) 540, 1370
BpmI CTGGAG 4 cut(s) 1083, 2112, 2203, 3122
Bpu1102I GCTNAGC 1 cut(s) 3481
Bpu14I TTCGAA 1 cut(s) 1923
BpuEI CTTGAG 3 cut(s) 1876, 2561, 3074
BpuMI CCSGG 2 cut(s) 954, 3237
Bsa29I ATCGAT 2 cut(s) 2374, 2547
BsaAI YACGTR 2 cut(s) 1976, 3197
BsaBI GATNNNNATC 1 cut(s) 2203
BsaHI GRCGYC 1 cut(s) 61
BsaI GGTCTC 1 cut(s) 3285
BsaJI CCNNGG 7 cut(s) 289, 1257, 1583, 2089, 2822, 2823, 3085
BsaWI WCCGGW 1 cut(s) 2689
BsaXI ACNNNNNCTCC 4 cut(s) 1333, 1363, 2201, 2231
Bsc4I CCNNNNNNNGG 6 cut(s) 1156, 1589, 2024, 2482, 3088, 3356
Bse1I ACTGG 8 cut(s) 115, 170, 272, 350, 1100, 1937, 2392, 3105
Bse21I CCTNAGG 1 cut(s) 3452
Bse3DI GCAATG 2 cut(s) 336, 3497
Bse8I GATNNNNATC 1 cut(s) 2203
BseBI CCWGG 5 cut(s) 1683, 2091, 2165, 2824, 3296
BseCI ATCGAT 2 cut(s) 2374, 2547
BseDI CCNNGG 7 cut(s) 289, 1257, 1583, 2089, 2822, 2823, 3085
BseGI GGATG 5 cut(s) 367, 1114, 1636, 2004, 3097
BseJI GATNNNNATC 1 cut(s) 2203
BseLI CCNNNNNNNGG 6 cut(s) 1156, 1589, 2024, 2482, 3088, 3356
BseMI GCAATG 2 cut(s) 336, 3497
BseMII CTCAG 8 cut(s) 881, 974, 1877, 2460, 2819, 2901, 3327, 3466
BseNI ACTGG 8 cut(s) 115, 170, 272, 350, 1100, 1937, 2392, 3105
BseSI GKGCMC 3 cut(s) 146, 954, 3608
BseYI CCCAGC 1 cut(s) 3326
BsgI GTGCAG 4 cut(s) 63, 1340, 3459, 3538
BshFI GGCC 1 cut(s) 3240
BshNI GGYRCC 2 cut(s) 1252, 2486
BshVI ATCGAT 2 cut(s) 2374, 2547
BsiHKAI GWGCWC 6 cut(s) 1382, 1427, 1747, 1972, 2275, 3608
BsiSI CCGG 4 cut(s) 323, 954, 2690, 3237
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 6 cut(s) 1156, 1589, 2024, 2482, 3088, 3356
BsmAI GTCTC 5 cut(s) 1799, 2806, 2906, 2928, 3285
BsmFI GGGAC 1 cut(s) 680
BsmI GAATGC 6 cut(s) 249, 1712, 1770, 1845, 2647, 3452
BsnI GGCC 1 cut(s) 3240
Bso31I GGTCTC 1 cut(s) 3285
Bsp119I TTCGAA 1 cut(s) 1923
Bsp1286I GDGCHC 8 cut(s) 146, 954, 1382, 1427, 1747, 1972, 2275, 3608
Bsp1720I GCTNAGC 1 cut(s) 3481
BspACI CCGC 6 cut(s) 1634, 2422, 2476, 3123, 3357, 3409
BspANI GGCC 1 cut(s) 3240
BspCNI CTCAG 8 cut(s) 880, 973, 1876, 2461, 2818, 2902, 3326, 3465
BspDI ATCGAT 2 cut(s) 2374, 2547
BspHI TCATGA 4 cut(s) 2404, 2439, 2992, 3229
BspLI GGNNCC 3 cut(s) 568, 1254, 2488
BspPI GGATC 8 cut(s) 249, 465, 1006, 1214, 1398, 2402, 2673, 3227
BspQI GCTCTTC 3 cut(s) 1128, 1215, 3324
BspT104I TTCGAA 1 cut(s) 1923
BspT107I GGYRCC 2 cut(s) 1252, 2486
BspTI CTTAAG 1 cut(s) 1007
BspTNI GGTCTC 1 cut(s) 3285
BsrDI GCAATG 2 cut(s) 336, 3497
BsrI ACTGG 8 cut(s) 115, 170, 272, 350, 1100, 1937, 2392, 3105
BssECI CCNNGG 7 cut(s) 289, 1257, 1583, 2089, 2822, 2823, 3085
BssNI GRCGYC 1 cut(s) 61
BssSI CACGAG 1 cut(s) 1785
BssT1I CCWWGG 3 cut(s) 289, 1257, 1583
Bst2BI CACGAG 1 cut(s) 1785
Bst2UI CCWGG 5 cut(s) 1683, 2091, 2165, 2824, 3296
Bst6I CTCTTC 5 cut(s) 746, 1128, 1215, 2421, 3324
BstACI GRCGYC 1 cut(s) 61
BstAFI CTTAAG 1 cut(s) 1007
BstAPI GCANNNNNTGC 3 cut(s) 812, 2653, 3276
BstBAI YACGTR 2 cut(s) 1976, 3197
BstBI TTCGAA 1 cut(s) 1923
BstEII GGTNACC 1 cut(s) 965
BstENI CCTNNNNNAGG 1 cut(s) 1154
BstF5I GGATG 5 cut(s) 367, 1114, 1636, 2004, 3097
BstHHI GCGC 1 cut(s) 423
BstMAI GTCTC 5 cut(s) 1799, 2806, 2906, 2928, 3285
BstNI CCWGG 5 cut(s) 1683, 2091, 2165, 2824, 3296
BstNSI RCATGY 4 cut(s) 132, 1991, 2246, 2713
BstPAI GACNNNNGTC 1 cut(s) 495
BstPI GGTNACC 1 cut(s) 965
BstSCI CCNGG 7 cut(s) 952, 1681, 2089, 2163, 2822, 3235, 3294
BstSFI CTRYAG 2 cut(s) 1371, 2898
BstSLI GKGCMC 3 cut(s) 146, 954, 3608
BstV2I GAAGAC 2 cut(s) 540, 1370
BstX2I RGATCY 3 cut(s) 1011, 2020, 3439
BstYI RGATCY 3 cut(s) 1011, 2020, 3439
Bsu15I ATCGAT 2 cut(s) 2374, 2547
Bsu36I CCTNAGG 1 cut(s) 3452
BsuRI GGCC 1 cut(s) 3240
BsuTUI ATCGAT 2 cut(s) 2374, 2547
BtrI CACGTC 2 cut(s) 2212, 3118
BtsCI GGATG 5 cut(s) 367, 1114, 1636, 2004, 3097
BtsI GCAGTG 3 cut(s) 51, 51, 484
CaiI CAGNNNCTG 4 cut(s) 194, 2812, 2909, 3276
CciI TCATGA 4 cut(s) 2404, 2439, 2992, 3229
CfoI GCGC 1 cut(s) 423
Cfr13I GGNCC 5 cut(s) 270, 566, 1685, 2782, 3239
ClaI ATCGAT 2 cut(s) 2374, 2547
CseI GACGC 2 cut(s) 665, 3410
CsiI ACCWGGT 1 cut(s) 2163
Csp6I GTAC 8 cut(s) 215, 1253, 1666, 2225, 2344, 3194, 3347, 3543
CviQI GTAC 8 cut(s) 215, 1253, 1666, 2225, 2344, 3194, 3347, 3543
DraI TTTAAA 1 cut(s) 2154
DraIII CACNNNGTG 2 cut(s) 1976, 3466
DrdI GACNNNNNNGTC 1 cut(s) 2920
DseDI GACNNNNNNGTC 1 cut(s) 2920
Eam1104I CTCTTC 5 cut(s) 746, 1128, 1215, 2421, 3324
EarI CTCTTC 5 cut(s) 746, 1128, 1215, 2421, 3324
Ecl136II GAGCTC 1 cut(s) 1745
Eco130I CCWWGG 3 cut(s) 289, 1257, 1583
Eco24I GRGCYC 1 cut(s) 1747
Eco31I GGTCTC 1 cut(s) 3285
Eco32I GATATC 2 cut(s) 436, 1954
Eco47I GGWCC 4 cut(s) 270, 566, 1685, 2782
Eco53kI GAGCTC 1 cut(s) 1745
Eco57I CTGAAG 4 cut(s) 1107, 1452, 2721, 3383
Eco81I CCTNAGG 1 cut(s) 3452
Eco91I GGTNACC 1 cut(s) 965
EcoICRI GAGCTC 1 cut(s) 1745
EcoNI CCTNNNNNAGG 1 cut(s) 1154
EcoO65I GGTNACC 1 cut(s) 965
EcoRI GAATTC 2 cut(s) 886, 2685
EcoRII CCWGG 5 cut(s) 1681, 2089, 2163, 2822, 3294
EcoRV GATATC 2 cut(s) 436, 1954
EcoT14I CCWWGG 3 cut(s) 289, 1257, 1583
EcoT22I ATGCAT 1 cut(s) 2715
EcoT38I GRGCYC 1 cut(s) 1747
ErhI CCWWGG 3 cut(s) 289, 1257, 1583
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 1 cut(s) 680
FauI CCCGC 1 cut(s) 3350
FauNDI CATATG 3 cut(s) 462, 2928, 3009
FbaI TGATCA 2 cut(s) 508, 1978
FblI GTMKAC 1 cut(s) 18
FokI GGATG 5 cut(s) 354, 1121, 1643, 2011, 3104
FriOI GRGCYC 1 cut(s) 1747
GlaI GCGC 1 cut(s) 422
GsaI CCCAGC 1 cut(s) 3330
GsuI CTGGAG 4 cut(s) 1083, 2112, 2203, 3122
HaeIII GGCC 1 cut(s) 3240
HapII CCGG 4 cut(s) 323, 954, 2690, 3237
HgaI GACGC 2 cut(s) 665, 3410
HhaI GCGC 1 cut(s) 423
Hin1I GRCGYC 1 cut(s) 61
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HincII GTYRAC 1 cut(s) 1930
HindII GTYRAC 1 cut(s) 1930
HindIII AAGCTT 2 cut(s) 1199, 2399
HpaII CCGG 4 cut(s) 323, 954, 2690, 3237
HphI GGTGA 9 cut(s) 92, 809, 977, 1512, 1851, 2179, 2411, 2729, 3452
HpyCH4IV ACGT 5 cut(s) 61, 1975, 2211, 3117, 3196
HpySE526I ACGT 5 cut(s) 61, 1975, 2211, 3117, 3196
Hsp92I GRCGYC 1 cut(s) 61
HspAI GCGC 1 cut(s) 421
KpnI GGTACC 1 cut(s) 1256
Ksp22I TGATCA 2 cut(s) 508, 1978
LguI GCTCTTC 3 cut(s) 1128, 1215, 3324
LmnI GCTCC 4 cut(s) 1742, 2093, 2116, 2278
MabI ACCWGGT 1 cut(s) 2163
MaeII ACGT 5 cut(s) 61, 1975, 2211, 3117, 3196
MaeIII GTNAC 9 cut(s) 46, 313, 965, 1018, 1518, 2107, 2326, 3308, 3458
MfeI CAATTG 4 cut(s) 576, 1164, 1692, 2726
MflI RGATCY 3 cut(s) 1011, 2020, 3439
MhlI GDGCHC 8 cut(s) 146, 954, 1382, 1427, 1747, 1972, 2275, 3608
MlyI GAGTC 3 cut(s) 485, 2930, 3305
MmeI TCCRAC 2 cut(s) 1319, 1597
Mph1103I ATGCAT 1 cut(s) 2715
MslI CAYNNNNRTG 2 cut(s) 1864, 3514
MspA1I CMGCKG 1 cut(s) 2558
MspCI CTTAAG 1 cut(s) 1007
MspI CCGG 4 cut(s) 323, 954, 2690, 3237
MspR9I CCNGG 7 cut(s) 954, 1683, 2091, 2165, 2824, 3237, 3296
MunI CAATTG 4 cut(s) 576, 1164, 1692, 2726
Mva1269I GAATGC 6 cut(s) 249, 1712, 1770, 1845, 2647, 3452
MvaI CCWGG 5 cut(s) 1683, 2091, 2165, 2824, 3296
NciI CCSGG 2 cut(s) 954, 3237
NdeI CATATG 3 cut(s) 462, 2928, 3009
NlaIV GGNNCC 3 cut(s) 568, 1254, 2488
NmuCI GTSAC 7 cut(s) 46, 313, 965, 1518, 2107, 3308, 3458
NsiI ATGCAT 1 cut(s) 2715
NspI RCATGY 4 cut(s) 132, 1991, 2246, 2713
NspV TTCGAA 1 cut(s) 1923
OliI CACNNNNGTG 1 cut(s) 1864
PaeI GCATGC 1 cut(s) 2713
PagI TCATGA 4 cut(s) 2404, 2439, 2992, 3229
PasI CCCWGGG 1 cut(s) 2823
PciI ACATGT 2 cut(s) 1987, 2242
PciSI GCTCTTC 3 cut(s) 1128, 1215, 3324
PctI GAATGC 6 cut(s) 249, 1712, 1770, 1845, 2647, 3452
PfeI GAWTC 7 cut(s) 638, 999, 1620, 2045, 2204, 2302, 3178
PfoI TCCNGGA 1 cut(s) 1681
PleI GAGTC 3 cut(s) 485, 2929, 3305
PpsI GAGTC 3 cut(s) 485, 2929, 3305
Ppu21I YACGTR 2 cut(s) 1976, 3197
PscI ACATGT 2 cut(s) 1987, 2242
PshAI GACNNNNGTC 1 cut(s) 495
PshBI ATTAAT 3 cut(s) 1044, 1659, 2034
PsiI TTATAA 1 cut(s) 1601
Psp124BI GAGCTC 1 cut(s) 1747
Psp6I CCWGG 5 cut(s) 1681, 2089, 2163, 2822, 3294
PspEI GGTNACC 1 cut(s) 965
PspFI CCCAGC 1 cut(s) 3326
PspGI CCWGG 5 cut(s) 1681, 2089, 2163, 2822, 3294
PspN4I GGNNCC 3 cut(s) 568, 1254, 2488
PspPI GGNCC 5 cut(s) 270, 566, 1685, 2782, 3239
PstNI CAGNNNCTG 4 cut(s) 194, 2812, 2909, 3276
PsuI RGATCY 3 cut(s) 1011, 2020, 3439
PvuII CAGCTG 1 cut(s) 2558
RsaI GTAC 8 cut(s) 216, 1254, 1667, 2226, 2345, 3195, 3348, 3544
RsaNI GTAC 8 cut(s) 215, 1253, 1666, 2225, 2344, 3194, 3347, 3543
RseI CAYNNNNRTG 2 cut(s) 1864, 3514
SacI GAGCTC 1 cut(s) 1747
SapI GCTCTTC 3 cut(s) 1128, 1215, 3324
Sau96I GGNCC 5 cut(s) 270, 566, 1685, 2782, 3239
SchI GAGTC 3 cut(s) 485, 2930, 3305
ScrFI CCNGG 7 cut(s) 954, 1683, 2091, 2165, 2824, 3237, 3296
SduI GDGCHC 8 cut(s) 146, 954, 1382, 1427, 1747, 1972, 2275, 3608
SexAI ACCWGGT 1 cut(s) 2163
SfcI CTRYAG 2 cut(s) 1371, 2898
SfuI TTCGAA 1 cut(s) 1923
SinI GGWCC 4 cut(s) 270, 566, 1685, 2782
SmiMI CAYNNNNRTG 2 cut(s) 1864, 3514
SmlI CTYRAG 4 cut(s) 1007, 1891, 2540, 3053
SmoI CTYRAG 4 cut(s) 1007, 1891, 2540, 3053
SpeI ACTAGT 2 cut(s) 656, 946
SphI GCATGC 1 cut(s) 2713
SsiI CCGC 6 cut(s) 1634, 2422, 2476, 3123, 3357, 3409
SspI AATATT 3 cut(s) 180, 1071, 2752
SstI GAGCTC 1 cut(s) 1747
StyD4I CCNGG 7 cut(s) 952, 1681, 2089, 2163, 2822, 3235, 3294
StyI CCWWGG 3 cut(s) 289, 1257, 1583
TaiI ACGT 5 cut(s) 64, 1978, 2214, 3120, 3199
TaqI TCGA 6 cut(s) 253, 1704, 1923, 2374, 2547, 3444
TatI WGTACW 2 cut(s) 1665, 2224
TauI GCSGC 1 cut(s) 3412
TfiI GAWTC 7 cut(s) 638, 999, 1620, 2045, 2204, 2302, 3178
TseFI GTSAC 7 cut(s) 46, 313, 965, 1518, 2107, 3308, 3458
Tsp45I GTSAC 7 cut(s) 46, 313, 965, 1518, 2107, 3308, 3458
TspGWI ACGGA 3 cut(s) 232, 1749, 2273
Vha464I CTTAAG 1 cut(s) 1007
VneI GTGCAC 1 cut(s) 3604
VpaK11BI GGWCC 4 cut(s) 270, 566, 1685, 2782
VspI ATTAAT 3 cut(s) 1044, 1659, 2034
XagI CCTNNNNNAGG 1 cut(s) 1154
XceI RCATGY 4 cut(s) 132, 1991, 2246, 2713
XcmI CCANNNNNNNNNTGG 1 cut(s) 2640
XmaJI CCTAGG 1 cut(s) 1583
XmiI GTMKAC 1 cut(s) 18
ZraI GACGTC 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 2715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.