pycom04g00450

ribosomal protein import into nucleus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004228
Physical Location & Seq
Reverse (-)
420584 .. 422508
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00450.1

Sequence Viewer

Length: 1479 bp
ATGGAGCCGGTATTTTCTCAGCAATTGCAAATGCAAGCCTACGCAACTCTGAGTTCGAACGATGTGCTGCCAATGGAGACGCTCCTTTCTCATCTCTTCAGCCCTCAAGAGCCCCAAAAATCTCAGGCCTATAGCCTTCTACATTGTTGCAGAAAGCACTTCCCGGATTTGCTTTTCATCAAGGTTTTCTTTGTGATTAGGAACGGGCCTTCCGCAGAGATTCGCACCAGGTCTGCGCTCGTTCTCCGGTTTGTGCTCGGTGACCTTTGGCCCAAGTTGTCTTTGAATGCCCAAATTAACCTTAAAAATTTATTTCTTGTTCGCCTTCAAGAAGAGGGCTCATTGCCCATTTTGAGGATTCTCTGTCTCATTGCATCTGAAATGGCAGTGGAGATTTCTAGGACTGGCGATGAGTGGCCGGAGCTCATTGACTATTTGTTGATGTCTTTTCAGTCGGACTCCGAAAGGTTTCAGCTTTCTGCTTTGTGGGTGTGTGCTTTTTTCCCAAAAGTGCATCGTCCGGTTATTTGTGAAACGTTAGCTCCTAGCATTGAACCTACCCACTCGGCATTTTTGCGTATGTTGAATTCGGAAAATGCTGATATTCAGGTTGCCACATTTGGTGCTGTGGTTAGTTTGATTCATTTGTTTTCGAATTCATTAGGGCGGAATTGGTTTCATGACCTTTTGAGAGGGATGATGGTTGGACTGTTTAATTTGTTAAGTCACTTGAAGGAAGACTATGCTCGCGGGGCTCTCAAGGAGTTAATAATGTTGGTAATGGAAGAGCCACAACTCTTGAAGCCATACCTTAACGAACTAGTACTTGATATGCTTAAGATAGTAGACAGTGAACAAGTGACAGAGGGAACCAAAGTGTTCGTTCACAAATTCTTGCTGACCGTAGTAGAAGCAAGCGATTTGGCACTTACAATGAGGGGACTACCAAATCAGACTTTGGTTAAACTGCTGGCAGTCCCAATGAAACTGCTTCTCTGTATAAATGATGACTATGCATGTTACAACACGGAAAGCGAGCAGGCTGAGAATGCTGGGAAGATAGATCTTGGAATTGCATACCTGACAAAAATATCAACTGCCTTAGGTGAAAAGACAATGACCCCCATAGCTTTTGAGTTGTTCTTGGAGTACATGGATGCTTCCGATTGGAAGAAGCGCCACGCAGGAATTTCTATGCTAGCAGTGATTGCAAAGGAGTGCTCAGAGGAGATGGTACTGATGAAAAATTATTTGGAACAAGTTACGACCATTATTTTGAAATCATTCCAAGATCCCCAGTCTCAAGTTTGTTGTGCAGCTTTTAATTTTATGCAACTGCCTATAATCCTGATTGAGGCAATGCAGATTCTTCATCATTTGAGGATTGTGCCTGCACTAGTTAGTGCACTTGACCAACATTCGATTCCAAGATTAAAGGTGCGGGTTTATGAATTTACTATCTGTTATGTTATTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

493

Amino Acids

55.87

Weight (kDa)

5.67

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_IPO5 PF25780 121 - 275 2.5e-11 IPO5-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 846
AccII CGCG 1 cut(s) 750
AciI CCGC 4 cut(s) 213, 667, 750, 1441
AclI AACGTT 1 cut(s) 536
AclWI GGATC 1 cut(s) 1286
AcoI YGGCCR 1 cut(s) 416
AcsI RAATTY 6 cut(s) 307, 586, 655, 890, 1188, 1451
AcuI CTGAAG 1 cut(s) 82
AfaI GTAC 3 cut(s) 825, 1151, 1236
AfiI CCNNNNNNNGG 2 cut(s) 354, 1354
AflII CTTAAG 1 cut(s) 836
AgsI TTSAA 7 cut(s) 286, 329, 554, 586, 733, 802, 1279
AhlI ACTAGT 2 cut(s) 820, 1396
AjnI CCWGG 1 cut(s) 227
AjuI GAANNNNNNNTTGG 4 cut(s) 1235, 1267, 1407, 1439
AloI GAACNNNNNNTCC 2 cut(s) 194, 226
AluBI AGCT 5 cut(s) 424, 475, 542, 1130, 1319
AluI AGCT 5 cut(s) 424, 475, 542, 1130, 1319
Alw21I GWGCWC 4 cut(s) 258, 426, 1223, 1408
Alw26I GTCTC 3 cut(s) 71, 371, 1305
Alw44I GTGCAC 1 cut(s) 1404
AlwI GGATC 1 cut(s) 1286
AoxI GGCC 4 cut(s) 126, 206, 269, 416
ApaLI GTGCAC 1 cut(s) 1404
ApeKI GCWGC 2 cut(s) 67, 1316
ApoI RAATTY 6 cut(s) 307, 586, 655, 890, 1188, 1451
ArsI GACNNNNNNTTYG 2 cut(s) 1258, 1290
Asp700I GAANNNNTTC 2 cut(s) 468, 1283
AspLEI GCGC 2 cut(s) 238, 1179
AspS9I GGNCC 2 cut(s) 206, 270
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 2 cut(s) 272, 1118
AsuII TTCGAA 2 cut(s) 56, 653
AsuNHI GCTAGC 1 cut(s) 1198
AxyI CCTNAGG 1 cut(s) 1102
BaeGI GKGCMC 1 cut(s) 1408
BanII GRGCYC 4 cut(s) 114, 341, 426, 757
BbsI GAAGAC 1 cut(s) 744
Bbv12I GWGCWC 4 cut(s) 258, 426, 1223, 1408
BbvI GCAGC 2 cut(s) 54, 1328
BccI CCATC 2 cut(s) 694, 1225
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciT130I CCWGG 1 cut(s) 229
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 3 cut(s) 71, 371, 1305
BcuI ACTAGT 2 cut(s) 820, 1396
BfaI CTAG 5 cut(s) 399, 546, 821, 1199, 1397
BfmI CTRYAG 1 cut(s) 130
BfoI RGCGCY 1 cut(s) 1180
BfrI CTTAAG 1 cut(s) 836
BglII AGATCT 1 cut(s) 1063
BisI GCNGC 2 cut(s) 68, 1317
BlsI GCNGC 2 cut(s) 69, 1318
BmcAI AGTACT 1 cut(s) 825
Bme1390I CCNGG 2 cut(s) 164, 229
BmgT120I GGNCC 2 cut(s) 206, 270
BmiI GGNNCC 2 cut(s) 6, 871
BmrFI CCNGG 2 cut(s) 164, 229
BmrI ACTGGG 1 cut(s) 1291
BmsI GCATC 3 cut(s) 383, 523, 1147
BmtI GCTAGC 1 cut(s) 1202
BmuI ACTGGG 1 cut(s) 1291
BpiI GAAGAC 1 cut(s) 744
Bpu14I TTCGAA 2 cut(s) 56, 653
BpuEI CTTGAG 3 cut(s) 90, 743, 1287
BpuMI CCSGG 1 cut(s) 164
BsaWI WCCGGW 2 cut(s) 246, 520
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 2 cut(s) 354, 1354
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 2 cut(s) 409, 1297
Bse21I CCTNAGG 1 cut(s) 1102
Bse3DI GCAATG 3 cut(s) 341, 369, 1365
BseBI CCWGG 1 cut(s) 229
BseGI GGATG 2 cut(s) 702, 1162
BseLI CCNNNNNNNGG 2 cut(s) 354, 1354
BseMI GCAATG 3 cut(s) 341, 369, 1365
BseMII CTCAG 5 cut(s) 32, 41, 137, 1035, 1236
BseNI ACTGG 2 cut(s) 409, 1297
BseRI GAGGAG 1 cut(s) 1241
BseSI GKGCMC 1 cut(s) 1408
BseXI GCAGC 2 cut(s) 54, 1328
BseYI CCCAGC 1 cut(s) 1052
BsgI GTGCAG 2 cut(s) 1335, 1377
Bsh1236I CGCG 1 cut(s) 750
BshFI GGCC 4 cut(s) 128, 208, 271, 418
BsiHKAI GWGCWC 4 cut(s) 258, 426, 1223, 1408
BsiSI CCGG 5 cut(s) 8, 164, 247, 419, 521
BslFI GGGAC 2 cut(s) 954, 962
BslI CCNNNNNNNGG 2 cut(s) 354, 1354
BsmAI GTCTC 3 cut(s) 71, 371, 1305
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 2 cut(s) 954, 962
BsmI GAATGC 2 cut(s) 292, 1054
BsnI GGCC 4 cut(s) 128, 208, 271, 418
Bsp119I TTCGAA 2 cut(s) 56, 653
Bsp1286I GDGCHC 7 cut(s) 114, 258, 341, 426, 757, 1223, 1408
Bsp143I GATC 2 cut(s) 1063, 1291
BspACI CCGC 4 cut(s) 213, 667, 750, 1441
BspANI GGCC 4 cut(s) 128, 208, 271, 418
BspCNI CTCAG 5 cut(s) 31, 42, 136, 1036, 1235
BspFNI CGCG 1 cut(s) 750
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 2 cut(s) 6, 871
BspOI GCTAGC 1 cut(s) 1202
BspPI GGATC 1 cut(s) 1286
BspQI GCTCTTC 1 cut(s) 780
BspT104I TTCGAA 2 cut(s) 56, 653
BspTI CTTAAG 1 cut(s) 836
BsrDI GCAATG 3 cut(s) 341, 369, 1365
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 2 cut(s) 409, 1297
BssAI RCCGGY 1 cut(s) 7
BssMI GATC 2 cut(s) 1063, 1291
Bst2UI CCWGG 1 cut(s) 229
Bst4CI ACNGT 3 cut(s) 711, 851, 904
Bst6I CTCTTC 3 cut(s) 101, 327, 780
BstAFI CTTAAG 1 cut(s) 836
BstAPI GCANNNNNTGC 1 cut(s) 1208
BstBI TTCGAA 2 cut(s) 56, 653
BstC8I GCNNGC 8 cut(s) 36, 748, 916, 972, 1037, 1041, 1200, 1392
BstDEI CTNAG 6 cut(s) 18, 50, 123, 1044, 1102, 1222
BstEII GGTNACC 1 cut(s) 260
BstENI CCTNNNNNAGG 1 cut(s) 1352
BstF5I GGATG 2 cut(s) 702, 1162
BstFNI CGCG 1 cut(s) 750
BstH2I RGCGCY 1 cut(s) 1180
BstHHI GCGC 2 cut(s) 238, 1179
BstKTI GATC 2 cut(s) 1066, 1294
BstMAI GTCTC 3 cut(s) 71, 371, 1305
BstMBI GATC 2 cut(s) 1063, 1291
BstMWI GCNNNNNNNGC 3 cut(s) 752, 1049, 1208
BstNI CCWGG 1 cut(s) 229
BstNSI RCATGY 1 cut(s) 1020
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 162, 227
BstSFI CTRYAG 1 cut(s) 130
BstSLI GKGCMC 1 cut(s) 1408
BstUI CGCG 1 cut(s) 750
BstV1I GCAGC 2 cut(s) 54, 1328
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 2 cut(s) 1063, 1291
BstYI RGATCY 2 cut(s) 1063, 1291
Bsu36I CCTNAGG 1 cut(s) 1102
BsuRI GGCC 4 cut(s) 128, 208, 271, 418
BtgZI GCGATG 1 cut(s) 423
BtsCI GGATG 2 cut(s) 702, 1162
BtsI GCAGTG 2 cut(s) 393, 1209
BtsIMutI CAGTG 3 cut(s) 393, 856, 1209
Cac8I GCNNGC 8 cut(s) 36, 748, 916, 972, 1037, 1041, 1200, 1392
CciI TCATGA 1 cut(s) 679
CfoI GCGC 2 cut(s) 238, 1179
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 206, 270
CseI GACGC 1 cut(s) 88
CsiI ACCWGGT 1 cut(s) 227
Csp6I GTAC 3 cut(s) 824, 1150, 1235
CspCI CAANNNNNGTGG 2 cut(s) 780, 815
CviAII CATG 3 cut(s) 680, 1017, 1153
CviQI GTAC 3 cut(s) 824, 1150, 1235
DdeI CTNAG 6 cut(s) 18, 50, 123, 1044, 1102, 1222
DpnI GATC 2 cut(s) 1065, 1293
DpnII GATC 2 cut(s) 1063, 1291
EaeI YGGCCR 1 cut(s) 416
Eam1104I CTCTTC 3 cut(s) 101, 327, 780
EarI CTCTTC 3 cut(s) 101, 327, 780
EciI GGCGGA 1 cut(s) 682
Ecl136II GAGCTC 1 cut(s) 424
Eco147I AGGCCT 1 cut(s) 128
Eco24I GRGCYC 4 cut(s) 114, 341, 426, 757
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 82
Eco81I CCTNAGG 1 cut(s) 1102
Eco91I GGTNACC 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 1352
EcoO65I GGTNACC 1 cut(s) 260
EcoRI GAATTC 2 cut(s) 586, 655
EcoRII CCWGG 1 cut(s) 227
EcoT22I ATGCAT 1 cut(s) 1018
EcoT38I GRGCYC 4 cut(s) 114, 341, 426, 757
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 3 cut(s) 683, 1020, 1156
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 2 cut(s) 954, 962
FatI CATG 3 cut(s) 679, 1016, 1152
FauI CCCGC 2 cut(s) 743, 1434
FblI GTMKAC 1 cut(s) 846
Fnu4HI GCNGC 2 cut(s) 68, 1317
FokI GGATG 2 cut(s) 709, 1169
FriOI GRGCYC 4 cut(s) 114, 341, 426, 757
Fsp4HI GCNGC 2 cut(s) 68, 1317
FspBI CTAG 5 cut(s) 399, 546, 821, 1199, 1397
GlaI GCGC 2 cut(s) 237, 1178
GluI GCNGC 2 cut(s) 68, 1317
GsaI CCCAGC 1 cut(s) 1056
HaeII RGCGCY 1 cut(s) 1180
HaeIII GGCC 4 cut(s) 128, 208, 271, 418
HapII CCGG 5 cut(s) 8, 164, 247, 419, 521
HgaI GACGC 1 cut(s) 88
HhaI GCGC 2 cut(s) 238, 1179
Hin1II CATG 3 cut(s) 683, 1020, 1156
Hin6I GCGC 2 cut(s) 236, 1177
HinP1I GCGC 2 cut(s) 236, 1177
HinfI GANTC 6 cut(s) 220, 358, 458, 640, 1366, 1423
HpaII CCGG 5 cut(s) 8, 164, 247, 419, 521
HphI GGTGA 2 cut(s) 272, 1118
Hpy166II GTNNAC 4 cut(s) 847, 854, 886, 1406
Hpy188I TCNGA 8 cut(s) 51, 379, 457, 463, 592, 954, 1165, 1225
Hpy188III TCNNGA 5 cut(s) 107, 329, 680, 799, 1348
Hpy8I GTNNAC 4 cut(s) 847, 854, 886, 1406
HpyAV CCTTC 4 cut(s) 146, 219, 335, 727
HpyCH4III ACNGT 3 cut(s) 711, 851, 904
HpyCH4IV ACGT 1 cut(s) 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 752, 1049, 1208
HpyF3I CTNAG 6 cut(s) 18, 50, 123, 1044, 1102, 1222
HpySE526I ACGT 1 cut(s) 536
Hsp92II CATG 3 cut(s) 683, 1020, 1156
HspAI GCGC 2 cut(s) 236, 1177
Kzo9I GATC 2 cut(s) 1063, 1291
LguI GCTCTTC 1 cut(s) 780
LmnI GCTCC 4 cut(s) 4, 87, 421, 547
Lsp1109I GCAGC 2 cut(s) 54, 1328
LweI GCATC 3 cut(s) 383, 523, 1147
MabI ACCWGGT 1 cut(s) 227
MaeI CTAG 5 cut(s) 399, 546, 821, 1199, 1397
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 5 cut(s) 260, 725, 859, 1019, 1261
MalI GATC 2 cut(s) 1065, 1293
MboI GATC 2 cut(s) 1063, 1291
MboII GAAGA 7 cut(s) 88, 344, 749, 797, 1069, 1183, 1361
MfeI CAATTG 1 cut(s) 23
MflI RGATCY 2 cut(s) 1063, 1291
MhlI GDGCHC 7 cut(s) 114, 258, 341, 426, 757, 1223, 1408
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 2 cut(s) 435, 685
MnlI CCTC 9 cut(s) 114, 328, 348, 686, 859, 930, 1219, 1348, 1374
Mph1103I ATGCAT 1 cut(s) 1018
MroXI GAANNNNTTC 2 cut(s) 468, 1283
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 5 cut(s) 8, 164, 247, 419, 521
MspR9I CCNGG 2 cut(s) 164, 229
MunI CAATTG 1 cut(s) 23
Mva1269I GAATGC 2 cut(s) 292, 1054
MvaI CCWGG 1 cut(s) 229
MvnI CGCG 1 cut(s) 750
MwoI GCNNNNNNNGC 3 cut(s) 752, 1049, 1208
NciI CCSGG 1 cut(s) 164
NdeII GATC 2 cut(s) 1063, 1291
NheI GCTAGC 1 cut(s) 1198
NlaIII CATG 3 cut(s) 683, 1020, 1156
NlaIV GGNNCC 2 cut(s) 6, 871
NmeAIII GCCGAG 1 cut(s) 545
NmuCI GTSAC 3 cut(s) 260, 725, 859
NsiI ATGCAT 1 cut(s) 1018
NspI RCATGY 1 cut(s) 1020
NspV TTCGAA 2 cut(s) 56, 653
PagI TCATGA 1 cut(s) 679
PceI AGGCCT 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 780
PctI GAATGC 2 cut(s) 292, 1054
PdmI GAANNNNTTC 2 cut(s) 468, 1283
PfeI GAWTC 5 cut(s) 220, 358, 640, 1366, 1423
PfoI TCCNGGA 1 cut(s) 162
PkrI GCNGC 2 cut(s) 69, 1318
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
Psp124BI GAGCTC 1 cut(s) 426
Psp1406I AACGTT 1 cut(s) 536
Psp6I CCWGG 1 cut(s) 227
PspEI GGTNACC 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 1052
PspGI CCWGG 1 cut(s) 227
PspN4I GGNNCC 2 cut(s) 6, 871
PspPI GGNCC 2 cut(s) 206, 270
PsuI RGATCY 2 cut(s) 1063, 1291
RsaI GTAC 3 cut(s) 825, 1151, 1236
RsaNI GTAC 3 cut(s) 824, 1150, 1235
SacI GAGCTC 1 cut(s) 426
SapI GCTCTTC 1 cut(s) 780
SatI GCNGC 2 cut(s) 68, 1317
Sau3AI GATC 2 cut(s) 1063, 1291
Sau96I GGNCC 2 cut(s) 206, 270
ScaI AGTACT 1 cut(s) 825
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 164, 229
SduI GDGCHC 7 cut(s) 114, 258, 341, 426, 757, 1223, 1408
SexAI ACCWGGT 1 cut(s) 227
SfaNI GCATC 3 cut(s) 383, 523, 1147
SfcI CTRYAG 1 cut(s) 130
SfuI TTCGAA 2 cut(s) 56, 653
SmlI CTYRAG 4 cut(s) 105, 758, 836, 1302
SmoI CTYRAG 4 cut(s) 105, 758, 836, 1302
SpeI ACTAGT 2 cut(s) 820, 1396
SseBI AGGCCT 1 cut(s) 128
SsiI CCGC 4 cut(s) 213, 667, 750, 1441
SspMI CTAG 5 cut(s) 399, 546, 821, 1199, 1397
SstI GAGCTC 1 cut(s) 426
StuI AGGCCT 1 cut(s) 128
StyD4I CCNGG 2 cut(s) 162, 227
TaaI ACNGT 3 cut(s) 711, 851, 904
TaiI ACGT 1 cut(s) 539
TaqI TCGA 3 cut(s) 56, 653, 1421
TatI WGTACW 2 cut(s) 823, 1149
TfiI GAWTC 5 cut(s) 220, 358, 640, 1366, 1423
TscAI CASTG 3 cut(s) 393, 856, 1209
TseFI GTSAC 3 cut(s) 260, 725, 859
TseI GCWGC 2 cut(s) 67, 1316
Tsp45I GTSAC 3 cut(s) 260, 725, 859
TspDTI ATGAA 8 cut(s) 166, 632, 648, 668, 998, 1256, 1361, 1464
TspGWI ACGGA 1 cut(s) 1043
TspRI CASTG 3 cut(s) 393, 856, 1209
Vha464I CTTAAG 1 cut(s) 836
VneI GTGCAC 1 cut(s) 1404
XagI CCTNNNNNAGG 1 cut(s) 1352
XapI RAATTY 6 cut(s) 307, 586, 655, 890, 1188, 1451
XceI RCATGY 1 cut(s) 1020
XmiI GTMKAC 1 cut(s) 846
XmnI GAANNNNTTC 2 cut(s) 468, 1283
XspI CTAG 5 cut(s) 399, 546, 821, 1199, 1397
ZrmI AGTACT 1 cut(s) 825
Zsp2I ATGCAT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.