Rh4DG005900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004228
Physical Location & Seq
Forward (+)
1048197 .. 1057351
9155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG005900.1

Sequence Viewer

Length: 477 bp
ATGGCAAGGTTTTTCCTGTGGCCCTTTCTTAGGGTTGAGGTCGTTGTTCAGCCGCGGGTTCTTGTCGTACCACCTCCAATTGTGGTCGTATCACCTCCTCCTGGTCCTGTGAAATTCGGTTTCTGGGAAGCTGTCACTGCTTTCTCGGGCACCATTGTTGCGGTATCGTCTGGTTTAACGCTTGCCAGCCAGAGGAAGCTTCTAGATTTGGAGTTTGGAAGGGTCAAAAGAGAAGTCTGCAAGGAGCAGAGGGAAGTGGAAGCTGCGAACAGAGAAGTCAGTAGGGAGAATCGCGAGATTGAAGCTGCAGAAAGAGAAGCCCGGAGGGAGGCGAGAGAAGTGGAGGCCGCTCACAGGGAAGTTGCCAGGTCTCAGAGAGAGATTGAGGCCTCAGAGAGAGAAGCCAAAAGGGAGAAGAACCGCTGGTGGAAGTCCAATGAGGAGCATCTAATCAACCTCTCCTATCGCCTCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

18.29

Weight (kDa)

9.29

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AccBSI CCGCTC 1 cut(s) 350
AccII CGCG 2 cut(s) 55, 294
AciI CCGC 5 cut(s) 53, 55, 161, 348, 421
AcsI RAATTY 1 cut(s) 113
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 5 cut(s) 30, 101, 192, 328, 354
AgsI TTSAA 1 cut(s) 302
AjnI CCWGG 2 cut(s) 100, 365
AluBI AGCT 4 cut(s) 131, 199, 263, 305
AluI AGCT 4 cut(s) 131, 199, 263, 305
Alw26I GTCTC 1 cut(s) 375
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 3 cut(s) 20, 345, 387
ApeKI GCWGC 2 cut(s) 263, 305
ApoI RAATTY 1 cut(s) 113
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
AspS9I GGNCC 2 cut(s) 21, 104
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 84
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 1 cut(s) 104
BaeGI GKGCMC 1 cut(s) 152
BanI GGYRCC 1 cut(s) 149
BbvI GCAGC 2 cut(s) 250, 292
BciT130I CCWGG 2 cut(s) 102, 367
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 375
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 306
BisI GCNGC 4 cut(s) 53, 264, 306, 348
BlsI GCNGC 4 cut(s) 54, 265, 307, 349
Bme1390I CCNGG 3 cut(s) 102, 322, 367
Bme18I GGWCC 1 cut(s) 104
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 21, 104
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 3 cut(s) 102, 322, 367
BmsI GCATC 1 cut(s) 454
BpuMI CCSGG 1 cut(s) 322
BsaI GGTCTC 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 53
Bsc4I CCNNNNNNNGG 5 cut(s) 30, 101, 192, 328, 354
BseBI CCWGG 2 cut(s) 102, 367
BseDI CCNNGG 1 cut(s) 53
BseLI CCNNNNNNNGG 5 cut(s) 30, 101, 192, 328, 354
BseMII CTCAG 2 cut(s) 386, 405
BseRI GAGGAG 2 cut(s) 87, 455
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 2 cut(s) 250, 292
Bsh1236I CGCG 2 cut(s) 55, 294
BshFI GGCC 3 cut(s) 22, 347, 389
BshNI GGYRCC 1 cut(s) 149
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 1 cut(s) 322
BslI CCNNNNNNNGG 5 cut(s) 30, 101, 192, 328, 354
BsmAI GTCTC 1 cut(s) 375
BsnI GGCC 3 cut(s) 22, 347, 389
Bso31I GGTCTC 1 cut(s) 375
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 1 cut(s) 152
Bsp68I TCGCGA 1 cut(s) 294
BspACI CCGC 5 cut(s) 53, 55, 161, 348, 421
BspANI GGCC 3 cut(s) 22, 347, 389
BspCNI CTCAG 2 cut(s) 385, 404
BspFNI CGCG 2 cut(s) 55, 294
BspLI GGNNCC 1 cut(s) 151
BspMAI CTGCAG 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 375
BsrBI CCGCTC 1 cut(s) 350
BssECI CCNNGG 1 cut(s) 53
Bst2UI CCWGG 2 cut(s) 102, 367
BstC8I GCNNGC 2 cut(s) 183, 187
BstDEI CTNAG 3 cut(s) 29, 372, 391
BstDSI CCRYGG 1 cut(s) 53
BstENI CCTNNNNNAGG 1 cut(s) 28
BstFNI CGCG 2 cut(s) 55, 294
BstMAI GTCTC 1 cut(s) 375
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstNI CCWGG 2 cut(s) 102, 367
BstSCI CCNGG 3 cut(s) 100, 320, 365
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 152
BstUI CGCG 2 cut(s) 55, 294
BstV1I GCAGC 2 cut(s) 250, 292
BsuRI GGCC 3 cut(s) 22, 347, 389
BtgI CCRYGG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
BtuMI TCGCGA 1 cut(s) 294
Cac8I GCNNGC 2 cut(s) 183, 187
Cfr13I GGNCC 2 cut(s) 21, 104
Cfr42I CCGCGG 1 cut(s) 56
Csp6I GTAC 1 cut(s) 68
CviQI GTAC 1 cut(s) 68
DdeI CTNAG 3 cut(s) 29, 372, 391
Eco147I AGGCCT 1 cut(s) 389
Eco31I GGTCTC 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 28
EcoRII CCWGG 2 cut(s) 100, 365
FaiI YATR 1 cut(s) 475
FauI CCCGC 1 cut(s) 48
Fnu4HI GCNGC 4 cut(s) 53, 264, 306, 348
Fsp4HI GCNGC 4 cut(s) 53, 264, 306, 348
FspBI CTAG 1 cut(s) 203
GluI GCNGC 4 cut(s) 53, 264, 306, 348
HaeIII GGCC 3 cut(s) 22, 347, 389
HapII CCGG 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 1 cut(s) 289
HpaII CCGG 1 cut(s) 322
HphI GGTGA 1 cut(s) 84
Hpy188I TCNGA 2 cut(s) 375, 394
Hpy188III TCNNGA 2 cut(s) 203, 293
HpyAV CCTTC 1 cut(s) 213
HpyCH4V TGCA 2 cut(s) 240, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 3 cut(s) 29, 372, 391
KspI CCGCGG 1 cut(s) 56
LmnI GCTCC 2 cut(s) 244, 442
Lsp1109I GCAGC 2 cut(s) 250, 292
LweI GCATC 1 cut(s) 454
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 133
MbiI CCGCTC 1 cut(s) 350
MboII GAAGA 1 cut(s) 427
MfeI CAATTG 1 cut(s) 78
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 2 cut(s) 78, 113
MseI TTAA 1 cut(s) 176
MspA1I CMGCKG 2 cut(s) 55, 423
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 3 cut(s) 102, 322, 367
MunI CAATTG 1 cut(s) 78
MvaI CCWGG 2 cut(s) 102, 367
MvnI CGCG 2 cut(s) 55, 294
MwoI GCNNNNNNNGC 1 cut(s) 137
NciI CCSGG 1 cut(s) 322
NlaIV GGNNCC 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 133
NruI TCGCGA 1 cut(s) 294
PceI AGGCCT 1 cut(s) 389
PfeI GAWTC 1 cut(s) 289
PkrI GCNGC 4 cut(s) 54, 265, 307, 349
Psp6I CCWGG 2 cut(s) 100, 365
PspGI CCWGG 2 cut(s) 100, 365
PspN4I GGNNCC 1 cut(s) 151
PspPI GGNCC 2 cut(s) 21, 104
PstI CTGCAG 1 cut(s) 310
RruI TCGCGA 1 cut(s) 294
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
SacII CCGCGG 1 cut(s) 56
SaqAI TTAA 1 cut(s) 176
SatI GCNGC 4 cut(s) 53, 264, 306, 348
Sau96I GGNCC 2 cut(s) 21, 104
ScrFI CCNGG 3 cut(s) 102, 322, 367
SduI GDGCHC 1 cut(s) 152
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 1 cut(s) 306
Sfr303I CCGCGG 1 cut(s) 56
SgrBI CCGCGG 1 cut(s) 56
SinI GGWCC 1 cut(s) 104
Sse9I AATT 2 cut(s) 78, 113
SseBI AGGCCT 1 cut(s) 389
SsiI CCGC 5 cut(s) 53, 55, 161, 348, 421
SspMI CTAG 1 cut(s) 203
StuI AGGCCT 1 cut(s) 389
StyD4I CCNGG 3 cut(s) 100, 320, 365
TasI AATT 2 cut(s) 78, 113
TauI GCSGC 2 cut(s) 55, 350
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 2 cut(s) 263, 305
Tsp45I GTSAC 1 cut(s) 133
TspRI CASTG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 104
XagI CCTNNNNNAGG 1 cut(s) 28
XapI RAATTY 1 cut(s) 113
XbaI TCTAGA 1 cut(s) 202
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.