Rw4G000410

ribosomal protein import into nucleus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004228
Physical Location & Seq
Forward (+)
966933 .. 970368
3436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000410.1

Sequence Viewer

Length: 1581 bp
ATGATGGAGTCGGAGTTTCCTCAGGAATTGCAAACACAAGCCTACGGAATTCTGAGCTCCAACGATGTTGAGCCATTGGAAACACTCTTTTCTCACGTGTTTGGCTCCCAAGAACCCCAAAGGTCCCAAGCTTACACCTTTTTACATTGTTGCAAAAAGCACTTCCCACATTTGCTCTTCATAAAACTCTTTTTTGTTATTAGATGTAGCCCTGTCTCTGACACTAGGTCCAGGTCTGCGCTTGTGCTTCGTTATCTCAAAATCTGTGACCTTTGGCCTAACTTGTCTCTAATTGCTCAAAATTACATGAAATCCCACTTCCTTGTTTGTCTTCAAGAGGAAGTTTCAATGCCCACTTTGAGACTACTTTGTGGCATTGTCTCTGAAATGGGCTCTGAGATTTCCAAGGCTGGCAATGAGTGGCCAGAGCTGATGGAGTTTCTGTTGAACTCTATTCAATCGGATTATGAAAAGTTGCAACAATGTGCATTATGGGTGTTGGCTTATTTGCCAAAAGGGTTCCGTCCGGTTGTTTGTGAAGCTTTGGTTCCTAGCCTTGAACCTTTACACTCGGCAATATTACAAGCCTTGAGTTCCTCCAATGCTGATATTCAGGTTACTGCATTTGGTGCTGTGGTTAGTTTGATAGGTTTGTTTACGAGTTCAAAGGGGCGGAACTGGTTTAATGACCTTTTGAGAGCAATGATGGTGGGACTGTTTAACTTGTTGAGTCACTTGAAAGAAGAATATGCTCGTGGGGCTGTCAATGAGTTAATAATGCTGGTCATGGATGAGCCTCAGATGTTGAAGCCATACCTTAACCATCTAGTACTTGACATGCTTAATGTGCTAGAGTGTAAAAAAGTGACAGATGAAACGAAACTGTTTGTTGACAAGTTCTTGCTCGCAATGGCAGAAGCCACGGATTTGGCACCTATGATGAGAGAGCTACCTCATGAAACAATGGTTAGGCTGCTCACGACCTCAATGCAACTGCTTCTCTCTATAAAGGAGGACAATGCATGGTACAACACGGAAAGCGAGCAGGGTGAGAATGATGGAAAGATGGATGTATACAAATCTGGAATTTTGTTCCTGAGAAAACTTTCAACGGCCTTAGGTGAAAAAAGAATACTTCCTATATGTTTTGAATTGTTCTTAGGGCACATGGAGGCTACCGAGTGGAAGAAACGTCATGCAGGGATTTCTTTGCTAGCAGTGATTGCCGAAGAGTGCTCAGATGAAATGGTTATGAGGAAAGACTGTTTGGACCAAGTTACTGCCATTATTTTGAAGTCATTCAAAGATCCTCATCCTCGAGTTTGTTGGGCAGCTTTTAATTTTATGCAACAGCCTACAAATCTGGTCCAGGCAATGCAAATACTCTATCATTTGAAGATTGTCTATGCACTGGACGCTGCACTTGACCAACACCGGAACCCAACAGTCAAGGAACAAGTTGTCTCAGCTTTACAATACTTGTTAAAGAATGTTCCTTCAGATAGCATACGAATGCACAAAGACTTGGATACTATATTGTGGAAGTTGCAGAAAGAACCACAGGTAGTTACAGCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

526

Amino Acids

60.01

Weight (kDa)

5.8

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_IPO5 PF25780 123 - 277 3.3e-15 IPO5-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1579
AccB1I GGYRCC 1 cut(s) 931
AccI GTMKAC 1 cut(s) 1074
AciI CCGC 1 cut(s) 673
AclWI GGATC 1 cut(s) 1301
AcoI YGGCCR 1 cut(s) 422
AcsI RAATTY 2 cut(s) 48, 1086
AcuI CTGAAG 1 cut(s) 1482
AcvI CACGTG 1 cut(s) 97
AfaI GTAC 2 cut(s) 831, 1028
AflIII ACRYGT 1 cut(s) 96
AhdI GACNNNNNGTC 1 cut(s) 226
AjnI CCWGG 2 cut(s) 230, 1368
AjuI GAANNNNNNNTTGG 2 cut(s) 1250, 1282
AluBI AGCT 8 cut(s) 57, 131, 430, 542, 949, 1334, 1469, 1574
AluI AGCT 8 cut(s) 57, 131, 430, 542, 949, 1334, 1469, 1574
Alw21I GWGCWC 2 cut(s) 59, 1238
Alw26I GTCTC 5 cut(s) 220, 291, 355, 385, 1468
AlwI GGATC 1 cut(s) 1301
Ama87I CYCGRG 1 cut(s) 1317
AoxI GGCC 3 cut(s) 275, 422, 1113
ApeKI GCWGC 3 cut(s) 973, 1331, 1418
ApoI RAATTY 2 cut(s) 48, 1086
Asp700I GAANNNNTTC 2 cut(s) 1105, 1298
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 4 cut(s) 123, 228, 1270, 1366
AsuHPI GGTGA 2 cut(s) 1061, 1133
AsuNHI GCTAGC 1 cut(s) 1213
AvaI CYCGRG 1 cut(s) 1317
AvaII GGWCC 4 cut(s) 123, 228, 1270, 1366
AxyI CCTNAGG 2 cut(s) 21, 1117
BaeGI GKGCMC 1 cut(s) 1167
BalI TGGCCA 1 cut(s) 424
BanI GGYRCC 1 cut(s) 931
BanII GRGCYC 2 cut(s) 59, 395
BauI CACGAG 1 cut(s) 753
BbrPI CACGTG 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 323
Bbv12I GWGCWC 2 cut(s) 59, 1238
BbvI GCAGC 3 cut(s) 960, 1343, 1405
BccI CCATC 5 cut(s) 427, 700, 831, 1052, 1060
BceAI ACGGC 1 cut(s) 1128
BcgI CGANNNNNNTGC 2 cut(s) 970, 1004
BciT130I CCWGG 2 cut(s) 232, 1370
BciVI GTATCC 1 cut(s) 1522
BcoDI GTCTC 5 cut(s) 220, 291, 355, 385, 1468
BfaI CTAG 5 cut(s) 225, 552, 827, 851, 1214
BfuI GTATCC 1 cut(s) 1522
BisI GCNGC 3 cut(s) 974, 1332, 1419
BlsI GCNGC 3 cut(s) 975, 1333, 1420
BmcAI AGTACT 1 cut(s) 831
Bme1390I CCNGG 2 cut(s) 232, 1370
Bme18I GGWCC 4 cut(s) 123, 228, 1270, 1366
BmeRI GACNNNNNGTC 1 cut(s) 226
BmeT110I CYCGRG 1 cut(s) 1317
BmgT120I GGNCC 4 cut(s) 123, 228, 1270, 1366
BmiI GGNNCC 6 cut(s) 106, 125, 521, 549, 933, 1439
BmrFI CCNGG 2 cut(s) 232, 1370
BmtI GCTAGC 1 cut(s) 1217
BpiI GAAGAC 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 610
BsaAI YACGTR 1 cut(s) 97
BsaBI GATNNNNATC 1 cut(s) 1311
BsaJI CCNNGG 2 cut(s) 405, 921
BsaWI WCCGGW 2 cut(s) 526, 1434
BsaXI ACNNNNNCTCC 3 cut(s) 29, 428, 458
Bse1I ACTGG 2 cut(s) 683, 1416
Bse21I CCTNAGG 2 cut(s) 21, 1117
Bse3DI GCAATG 4 cut(s) 421, 708, 915, 1380
Bse8I GATNNNNATC 1 cut(s) 1311
BseBI CCWGG 2 cut(s) 232, 1370
BseDI CCNNGG 2 cut(s) 405, 921
BseGI GGATG 3 cut(s) 796, 1075, 1312
BseJI GATNNNNATC 1 cut(s) 1311
BseMI GCAATG 4 cut(s) 421, 708, 915, 1380
BseMII CTCAG 7 cut(s) 35, 44, 387, 812, 1088, 1251, 1479
BseNI ACTGG 2 cut(s) 683, 1416
BseSI GKGCMC 1 cut(s) 1167
BseXI GCAGC 3 cut(s) 960, 1343, 1405
BsgI GTGCAG 1 cut(s) 1404
BshFI GGCC 3 cut(s) 277, 424, 1115
BshNI GGYRCC 1 cut(s) 931
BsiHKAI GWGCWC 2 cut(s) 59, 1238
BsiHKCI CYCGRG 1 cut(s) 1317
BsiSI CCGG 2 cut(s) 527, 1435
BslFI GGGAC 2 cut(s) 109, 726
BsmAI GTCTC 5 cut(s) 220, 291, 355, 385, 1468
BsmFI GGGAC 2 cut(s) 109, 726
BsmI GAATGC 1 cut(s) 1518
BsnI GGCC 3 cut(s) 277, 424, 1115
BsoBI CYCGRG 1 cut(s) 1317
Bsp1286I GDGCHC 4 cut(s) 59, 395, 1167, 1238
Bsp143I GATC 1 cut(s) 1306
BspACI CCGC 1 cut(s) 673
BspANI GGCC 3 cut(s) 277, 424, 1115
BspCNI CTCAG 7 cut(s) 34, 45, 388, 811, 1089, 1250, 1478
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 6 cut(s) 106, 125, 521, 549, 933, 1439
BspOI GCTAGC 1 cut(s) 1217
BspPI GGATC 1 cut(s) 1301
BspQI GCTCTTC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 931
BsrDI GCAATG 4 cut(s) 421, 708, 915, 1380
BsrI ACTGG 2 cut(s) 683, 1416
BssECI CCNNGG 2 cut(s) 405, 921
BssMI GATC 1 cut(s) 1306
BssNAI GTATAC 1 cut(s) 1075
BssSI CACGAG 1 cut(s) 753
BssT1I CCWWGG 1 cut(s) 405
Bst1107I GTATAC 1 cut(s) 1075
Bst2BI CACGAG 1 cut(s) 753
Bst2UI CCWGG 2 cut(s) 232, 1370
Bst4CI ACNGT 4 cut(s) 717, 885, 1265, 1447
Bst6I CTCTTC 2 cut(s) 182, 1224
BstAPI GCANNNNNTGC 2 cut(s) 629, 1223
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 4 cut(s) 412, 906, 1043, 1215
BstDEI CTNAG 9 cut(s) 21, 53, 396, 798, 1097, 1117, 1159, 1237, 1465
BstDSI CCRYGG 1 cut(s) 921
BstF5I GGATG 3 cut(s) 796, 1075, 1312
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 1 cut(s) 1309
BstMAI GTCTC 5 cut(s) 220, 291, 355, 385, 1468
BstMBI GATC 1 cut(s) 1306
BstMWI GCNNNNNNNGC 5 cut(s) 629, 758, 847, 1223, 1415
BstNI CCWGG 2 cut(s) 232, 1370
BstNSI RCATGY 1 cut(s) 841
BstSCI CCNGG 2 cut(s) 230, 1368
BstSLI GKGCMC 1 cut(s) 1167
BstV1I GCAGC 3 cut(s) 960, 1343, 1405
BstV2I GAAGAC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 1306
BstXI CCANNNNNNTGG 1 cut(s) 928
BstYI RGATCY 1 cut(s) 1306
BstZ17I GTATAC 1 cut(s) 1075
Bsu36I CCTNAGG 2 cut(s) 21, 1117
BsuI GTATCC 1 cut(s) 1522
BsuRI GGCC 3 cut(s) 277, 424, 1115
BtgI CCRYGG 1 cut(s) 921
BtsCI GGATG 3 cut(s) 796, 1075, 1312
BtsI GCAGTG 1 cut(s) 1224
BtsIMutI CAGTG 2 cut(s) 1224, 1409
Cac8I GCNNGC 4 cut(s) 412, 906, 1043, 1215
CciI TCATGA 1 cut(s) 955
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 4 cut(s) 123, 228, 1270, 1366
CseI GACGC 1 cut(s) 1424
Csp6I GTAC 2 cut(s) 830, 1027
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviAII CATG 7 cut(s) 307, 787, 838, 956, 1023, 1168, 1196
CviQI GTAC 2 cut(s) 830, 1027
DdeI CTNAG 9 cut(s) 21, 53, 396, 798, 1097, 1117, 1159, 1237, 1465
DpnI GATC 1 cut(s) 1308
DpnII GATC 1 cut(s) 1306
DriI GACNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 422
Eam1104I CTCTTC 2 cut(s) 182, 1224
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 2 cut(s) 182, 1224
EciI GGCGGA 1 cut(s) 688
Ecl136II GAGCTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 2 cut(s) 59, 395
Eco47I GGWCC 4 cut(s) 123, 228, 1270, 1366
Eco53kI GAGCTC 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 1482
Eco72I CACGTG 1 cut(s) 97
Eco81I CCTNAGG 2 cut(s) 21, 1117
Eco88I CYCGRG 1 cut(s) 1317
EcoICRI GAGCTC 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 123
EcoRI GAATTC 1 cut(s) 48
EcoRII CCWGG 2 cut(s) 230, 1368
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 1 cut(s) 1024
EcoT38I GRGCYC 2 cut(s) 59, 395
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 7 cut(s) 310, 790, 841, 959, 1026, 1171, 1199
FaqI GGGAC 2 cut(s) 109, 726
FatI CATG 7 cut(s) 306, 786, 837, 955, 1022, 1167, 1195
FblI GTMKAC 1 cut(s) 1074
Fnu4HI GCNGC 3 cut(s) 974, 1332, 1419
FokI GGATG 3 cut(s) 803, 1082, 1299
FriOI GRGCYC 2 cut(s) 59, 395
Fsp4HI GCNGC 3 cut(s) 974, 1332, 1419
FspBI CTAG 5 cut(s) 225, 552, 827, 851, 1214
GlaI GCGC 1 cut(s) 240
GluI GCNGC 3 cut(s) 974, 1332, 1419
HaeIII GGCC 3 cut(s) 277, 424, 1115
HapII CCGG 2 cut(s) 527, 1435
HgaI GACGC 1 cut(s) 1424
HhaI GCGC 1 cut(s) 241
Hin1II CATG 7 cut(s) 310, 790, 841, 959, 1026, 1171, 1199
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 2 cut(s) 129, 540
HinfI GANTC 2 cut(s) 8, 730
HpaII CCGG 2 cut(s) 527, 1435
HphI GGTGA 2 cut(s) 1061, 1133
Hpy166II GTNNAC 3 cut(s) 657, 892, 1075
Hpy188I TCNGA 9 cut(s) 13, 54, 220, 385, 397, 463, 801, 1240, 1501
Hpy188III TCNNGA 6 cut(s) 23, 335, 956, 979, 1083, 1096
Hpy8I GTNNAC 3 cut(s) 657, 892, 1075
HpyAV CCTTC 1 cut(s) 1506
HpyCH4III ACNGT 4 cut(s) 717, 885, 1265, 1447
HpyCH4IV ACGT 2 cut(s) 96, 1192
HpyF10VI GCNNNNNNNGC 5 cut(s) 629, 758, 847, 1223, 1415
HpyF3I CTNAG 9 cut(s) 21, 53, 396, 798, 1097, 1117, 1159, 1237, 1465
HpySE526I ACGT 2 cut(s) 96, 1192
Hsp92II CATG 7 cut(s) 310, 790, 841, 959, 1026, 1171, 1199
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 1 cut(s) 1306
LguI GCTCTTC 1 cut(s) 182
LmnI GCTCC 2 cut(s) 62, 110
Lsp1109I GCAGC 3 cut(s) 960, 1343, 1405
MaeI CTAG 5 cut(s) 225, 552, 827, 851, 1214
MaeII ACGT 2 cut(s) 96, 1192
MaeIII GTNAC 6 cut(s) 266, 616, 731, 865, 1276, 1567
MalI GATC 1 cut(s) 1308
MboI GATC 1 cut(s) 1306
MboII GAAGA 6 cut(s) 169, 323, 755, 1198, 1241, 1408
MflI RGATCY 1 cut(s) 1306
MhlI GDGCHC 4 cut(s) 59, 395, 1167, 1238
MlsI TGGCCA 1 cut(s) 424
MluCI AATT 7 cut(s) 26, 48, 291, 301, 1086, 1151, 1339
MluNI TGGCCA 1 cut(s) 424
MlyI GAGTC 2 cut(s) 17, 739
MmeI TCCRAC 1 cut(s) 84
Mox20I TGGCCA 1 cut(s) 424
Mph1103I ATGCAT 1 cut(s) 1024
MroXI GAANNNNTTC 2 cut(s) 1105, 1298
MscI TGGCCA 1 cut(s) 424
MseI TTAA 7 cut(s) 684, 720, 773, 819, 843, 1338, 1484
MslI CAYNNNNRTG 1 cut(s) 1511
Msp20I TGGCCA 1 cut(s) 424
MspI CCGG 2 cut(s) 527, 1435
MspR9I CCNGG 2 cut(s) 232, 1370
Mva1269I GAATGC 1 cut(s) 1518
MvaI CCWGG 2 cut(s) 232, 1370
MwoI GCNNNNNNNGC 5 cut(s) 629, 758, 847, 1223, 1415
NdeII GATC 1 cut(s) 1306
NheI GCTAGC 1 cut(s) 1213
NlaIII CATG 7 cut(s) 310, 790, 841, 959, 1026, 1171, 1199
NlaIV GGNNCC 6 cut(s) 106, 125, 521, 549, 933, 1439
NmeAIII GCCGAG 1 cut(s) 551
NmuCI GTSAC 3 cut(s) 266, 731, 865
NsiI ATGCAT 1 cut(s) 1024
NspI RCATGY 1 cut(s) 841
PaeR7I CTCGAG 1 cut(s) 1317
PagI TCATGA 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 182
PctI GAATGC 1 cut(s) 1518
PdmI GAANNNNTTC 2 cut(s) 1105, 1298
PkrI GCNGC 3 cut(s) 975, 1333, 1420
PleI GAGTC 2 cut(s) 16, 738
PmaCI CACGTG 1 cut(s) 97
PmlI CACGTG 1 cut(s) 97
PpsI GAGTC 2 cut(s) 16, 738
Ppu21I YACGTR 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 123
PsiI TTATAA 1 cut(s) 1579
Psp124BI GAGCTC 1 cut(s) 59
Psp5II RGGWCCY 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 230, 1368
PspCI CACGTG 1 cut(s) 97
PspGI CCWGG 2 cut(s) 230, 1368
PspN4I GGNNCC 6 cut(s) 106, 125, 521, 549, 933, 1439
PspPI GGNCC 4 cut(s) 123, 228, 1270, 1366
PspPPI RGGWCCY 1 cut(s) 123
PspXI VCTCGAGB 1 cut(s) 1317
PsuI RGATCY 1 cut(s) 1306
RsaI GTAC 2 cut(s) 831, 1028
RsaNI GTAC 2 cut(s) 830, 1027
RseI CAYNNNNRTG 1 cut(s) 1511
SacI GAGCTC 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 182
SaqAI TTAA 7 cut(s) 684, 720, 773, 819, 843, 1338, 1484
SatI GCNGC 3 cut(s) 974, 1332, 1419
Sau3AI GATC 1 cut(s) 1306
Sau96I GGNCC 4 cut(s) 123, 228, 1270, 1366
ScaI AGTACT 1 cut(s) 831
SchI GAGTC 2 cut(s) 17, 739
ScrFI CCNGG 2 cut(s) 232, 1370
SduI GDGCHC 4 cut(s) 59, 395, 1167, 1238
Sfr274I CTCGAG 1 cut(s) 1317
SinI GGWCC 4 cut(s) 123, 228, 1270, 1366
SlaI CTCGAG 1 cut(s) 1317
SmiMI CAYNNNNRTG 1 cut(s) 1511
SmlI CTYRAG 2 cut(s) 589, 1317
SmoI CTYRAG 2 cut(s) 589, 1317
Sse9I AATT 7 cut(s) 26, 48, 291, 301, 1086, 1151, 1339
SsiI CCGC 1 cut(s) 673
SspI AATATT 1 cut(s) 579
SspMI CTAG 5 cut(s) 225, 552, 827, 851, 1214
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 2 cut(s) 230, 1368
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 4 cut(s) 717, 885, 1265, 1447
TaiI ACGT 2 cut(s) 99, 1195
TaqI TCGA 1 cut(s) 1318
TasI AATT 7 cut(s) 26, 48, 291, 301, 1086, 1151, 1339
TatI WGTACW 1 cut(s) 829
Tru1I TTAA 7 cut(s) 684, 720, 773, 819, 843, 1338, 1484
Tru9I TTAA 7 cut(s) 684, 720, 773, 819, 843, 1338, 1484
TscAI CASTG 2 cut(s) 1224, 1416
TseFI GTSAC 3 cut(s) 266, 731, 865
TseI GCWGC 3 cut(s) 973, 1331, 1418
Tsp45I GTSAC 3 cut(s) 266, 731, 865
TspDTI ATGAA 6 cut(s) 169, 323, 483, 888, 972, 1257
TspGWI ACGGA 4 cut(s) 60, 512, 938, 1049
TspRI CASTG 2 cut(s) 1224, 1416
VpaK11BI GGWCC 4 cut(s) 123, 228, 1270, 1366
XapI RAATTY 2 cut(s) 48, 1086
XceI RCATGY 1 cut(s) 841
XhoI CTCGAG 1 cut(s) 1317
XmiI GTMKAC 1 cut(s) 1074
XmnI GAANNNNTTC 2 cut(s) 1105, 1298
XspI CTAG 5 cut(s) 225, 552, 827, 851, 1214
ZrmI AGTACT 1 cut(s) 831
Zsp2I ATGCAT 1 cut(s) 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.