Rw4G000420

ribosomal protein import into nucleus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004228
Physical Location & Seq
Forward (+)
1004646 .. 1007855
3210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000420.1

Sequence Viewer

Length: 1551 bp
ATGATGGAGTCGGAGTTTCCTCGGGAATTGCAAACACAAGCCTACGGAATTCTGAGCTCCAACGATGTTGAGCCATTGGAGACACTCTTTTCTCACGTGTTTGGCTCCCAAGAACCCCAAAGGTCCCAAGCTTACACCTTTTTACATTGTTGCAAAAAGCACTTCCCACATTTGCTCTTCATAAAACTCTTTTTTGTTATTAGATGTAGCCCTGTCTCTGACACTAGGTCCAGGTCTGCGCTTGTGCTTCGTTATCTCAAAATCTGTGACCTTTGGCCTACCTTGTCAGTAATTGCTCAAAATTACATGAAATCCCACTTCCTTGTTTGTCTTCAAGAGGAAGTTTCAATGCCCACTTTGAGACTACTTTGTGGCATTGCTGGCAATGAGTGGCCAGAGCTCACGGAGTTTTTGTTGAATTCTATTCAATCGGATTATGAGAAGTTACAACAATCTGCATTATGGGTGTTCGCTTATTTACCAAAAGGGTTCCGTCCGGTTGTTTGTGAAGCTTTACTTCCTATCCTTGAACCTATACACTCGGCAATATTACAAGCGTTGAGTTCCCCCAATGCAGATATTCAGGTTACAGCATTTGGTGCTGTGGTCAGTTTGATAGGTTTGTTTACGAGTTCAAAGGGGCGGAACTGGTTTAATGACCTTTTGAGAGCAATGATGTTGGGACTGTTTAACTTGTTGAGTCACTTGAAAGAAGAATATGCTCGTGGGGCTGTCAATGAGTTAATAATGCTGGTCATGGATGAGCCACAAATCTTGAAGCCTTACCTTAACCATCTAGTGCTCGACATGCTTAATGTGCTAGAGTGTAAAAAAGTGACACATGAAACGAAACTGTTTGTTGACAAGTTCTCGCTCGCAATGGCAGAAGCCACGGATTTGGCACCTATGATGAGAGAACTACCTCATGAAACAATGTTTAGGCTGCTCACGACCTCAATGCAACTGCTTCTCTCTATAAAGGATGACAATGCATGGTACAACACTGAAAGCGAGCAGGGTGAGAATGATGGAAAGATGGATGTATACAAATCTGGGATTTTGTTCCTGAGGAAACTTTCAACGGCCTTAGATGAAAAAAGAATACTTCCTATATGTTTTGAATTGTTCTTGGGGCACATGGAGGCTACCGAGTGGAAGAAACGTTATGCGGGGATTTCTTTGCTAGCAGTGATTGCCGAGGAGTGCTCAGATGAAATGGTTATGAGGAAAGACTGTTTGGACCAAGTTATTGCCATTATTTTGAAGTCATTCAAAGATCCTCATCCTCGAGTTTGTTGGGCAGCTTTTAATTTTATGCAGCAGCCTACAAATCTGGTCCAGACAATGCAAATTCTCTATCATTTGAAGATTGTCTATGCACTGGACGCTGCACTTGACCAACACCGGAACCCAACAGTCAAGGAACAAGTTGTCTCAGCTTTACAATACTTGTTAAAGAATGTTCCTTCAGATAGCTTAAGAATGCACACAGACTTGGATACTATATTCTGGAAGTTGCAGAAAGAACCACAGGTAGTTACAGCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

516

Amino Acids

59.16

Weight (kDa)

5.87

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_IPO5 PF25780 128 - 267 1.1e-12 IPO5-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1549
AccB1I GGYRCC 1 cut(s) 901
AccI GTMKAC 1 cut(s) 1044
AciI CCGC 2 cut(s) 643, 1169
AclI AACGTT 1 cut(s) 1162
AclWI GGATC 1 cut(s) 1271
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 3 cut(s) 48, 418, 1350
AcuI CTGAAG 1 cut(s) 1452
AcvI CACGTG 1 cut(s) 97
AfaI GTAC 1 cut(s) 998
AflII CTTAAG 1 cut(s) 1477
AflIII ACRYGT 1 cut(s) 96
AhdI GACNNNNNGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 230
AjuI GAANNNNNNNTTGG 2 cut(s) 1220, 1252
AluBI AGCT 8 cut(s) 57, 131, 400, 512, 1304, 1439, 1476, 1544
AluI AGCT 8 cut(s) 57, 131, 400, 512, 1304, 1439, 1476, 1544
Alw21I GWGCWC 4 cut(s) 59, 402, 804, 1208
Alw26I GTCTC 4 cut(s) 74, 220, 355, 1438
AlwI GGATC 1 cut(s) 1271
Ama87I CYCGRG 2 cut(s) 21, 1287
AoxI GGCC 3 cut(s) 275, 392, 1083
ApeKI GCWGC 5 cut(s) 943, 1301, 1318, 1321, 1388
ApoI RAATTY 3 cut(s) 48, 418, 1350
Asp700I GAANNNNTTC 1 cut(s) 1268
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 4 cut(s) 123, 228, 1240, 1336
AsuHPI GGTGA 1 cut(s) 1031
AsuNHI GCTAGC 1 cut(s) 1183
AvaI CYCGRG 2 cut(s) 21, 1287
AvaII GGWCC 4 cut(s) 123, 228, 1240, 1336
AxyI CCTNAGG 1 cut(s) 1067
BaeGI GKGCMC 1 cut(s) 1137
BalI TGGCCA 1 cut(s) 394
BanI GGYRCC 1 cut(s) 901
BanII GRGCYC 2 cut(s) 59, 402
BauI CACGAG 1 cut(s) 723
BbrPI CACGTG 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 323
Bbv12I GWGCWC 4 cut(s) 59, 402, 804, 1208
BbvI GCAGC 5 cut(s) 930, 1313, 1330, 1333, 1375
BccI CCATC 3 cut(s) 801, 1022, 1030
BceAI ACGGC 1 cut(s) 1098
BcgI CGANNNNNNTGC 2 cut(s) 940, 974
BciT130I CCWGG 1 cut(s) 232
BciVI GTATCC 1 cut(s) 1492
BcoDI GTCTC 4 cut(s) 74, 220, 355, 1438
BfaI CTAG 4 cut(s) 225, 797, 821, 1184
BfrI CTTAAG 1 cut(s) 1477
BfuI GTATCC 1 cut(s) 1492
BisI GCNGC 5 cut(s) 944, 1302, 1319, 1322, 1389
BlsI GCNGC 5 cut(s) 945, 1303, 1320, 1323, 1390
Bme1390I CCNGG 1 cut(s) 232
Bme18I GGWCC 4 cut(s) 123, 228, 1240, 1336
BmeRI GACNNNNNGTC 1 cut(s) 226
BmeT110I CYCGRG 2 cut(s) 21, 1287
BmgT120I GGNCC 4 cut(s) 123, 228, 1240, 1336
BmiI GGNNCC 5 cut(s) 106, 125, 491, 903, 1409
BmrFI CCNGG 1 cut(s) 232
BmtI GCTAGC 1 cut(s) 1187
BpiI GAAGAC 1 cut(s) 323
BplI GAGNNNNNCTC 2 cut(s) 1190, 1222
BsaAI YACGTR 1 cut(s) 97
BsaBI GATNNNNATC 1 cut(s) 1281
BsaJI CCNNGG 3 cut(s) 20, 891, 1197
BsaWI WCCGGW 2 cut(s) 496, 1404
BsaXI ACNNNNNCTCC 3 cut(s) 29, 398, 428
Bse1I ACTGG 2 cut(s) 653, 1386
Bse21I CCTNAGG 1 cut(s) 1067
Bse3DI GCAATG 4 cut(s) 375, 391, 678, 885
Bse8I GATNNNNATC 1 cut(s) 1281
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 3 cut(s) 20, 891, 1197
BseGI GGATG 4 cut(s) 766, 988, 1045, 1282
BseJI GATNNNNATC 1 cut(s) 1281
BseMI GCAATG 4 cut(s) 375, 391, 678, 885
BseMII CTCAG 4 cut(s) 44, 1058, 1221, 1449
BseNI ACTGG 2 cut(s) 653, 1386
BseRI GAGGAG 1 cut(s) 1214
BseSI GKGCMC 1 cut(s) 1137
BseXI GCAGC 5 cut(s) 930, 1313, 1330, 1333, 1375
BsgI GTGCAG 1 cut(s) 1374
BshFI GGCC 3 cut(s) 277, 394, 1085
BshNI GGYRCC 1 cut(s) 901
BsiHKAI GWGCWC 4 cut(s) 59, 402, 804, 1208
BsiHKCI CYCGRG 2 cut(s) 21, 1287
BsiSI CCGG 2 cut(s) 497, 1405
BslFI GGGAC 2 cut(s) 109, 696
BsmAI GTCTC 4 cut(s) 74, 220, 355, 1438
BsmFI GGGAC 2 cut(s) 109, 696
BsmI GAATGC 1 cut(s) 1488
BsnI GGCC 3 cut(s) 277, 394, 1085
BsoBI CYCGRG 2 cut(s) 21, 1287
Bsp1286I GDGCHC 5 cut(s) 59, 402, 804, 1137, 1208
Bsp143I GATC 1 cut(s) 1276
BspACI CCGC 2 cut(s) 643, 1169
BspANI GGCC 3 cut(s) 277, 394, 1085
BspCNI CTCAG 4 cut(s) 45, 1059, 1220, 1448
BspHI TCATGA 1 cut(s) 925
BspLI GGNNCC 5 cut(s) 106, 125, 491, 903, 1409
BspOI GCTAGC 1 cut(s) 1187
BspPI GGATC 1 cut(s) 1271
BspQI GCTCTTC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 901
BspTI CTTAAG 1 cut(s) 1477
BsrDI GCAATG 4 cut(s) 375, 391, 678, 885
BsrI ACTGG 2 cut(s) 653, 1386
BssECI CCNNGG 3 cut(s) 20, 891, 1197
BssMI GATC 1 cut(s) 1276
BssNAI GTATAC 1 cut(s) 1045
BssSI CACGAG 1 cut(s) 723
Bst1107I GTATAC 1 cut(s) 1045
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 4 cut(s) 687, 855, 1235, 1417
Bst6I CTCTTC 1 cut(s) 182
BstAFI CTTAAG 1 cut(s) 1477
BstAPI GCANNNNNTGC 2 cut(s) 599, 1193
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 4 cut(s) 382, 876, 1013, 1185
BstDEI CTNAG 5 cut(s) 53, 1067, 1087, 1207, 1435
BstDSI CCRYGG 1 cut(s) 891
BstF5I GGATG 4 cut(s) 766, 988, 1045, 1282
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 1 cut(s) 1279
BstMAI GTCTC 4 cut(s) 74, 220, 355, 1438
BstMBI GATC 1 cut(s) 1276
BstMWI GCNNNNNNNGC 7 cut(s) 381, 599, 728, 808, 817, 1193, 1385
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 1 cut(s) 230
BstSLI GKGCMC 1 cut(s) 1137
BstV1I GCAGC 5 cut(s) 930, 1313, 1330, 1333, 1375
BstV2I GAAGAC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 1276
BstXI CCANNNNNNTGG 1 cut(s) 898
BstYI RGATCY 1 cut(s) 1276
BstZ17I GTATAC 1 cut(s) 1045
Bsu36I CCTNAGG 1 cut(s) 1067
BsuI GTATCC 1 cut(s) 1492
BsuRI GGCC 3 cut(s) 277, 394, 1085
BtgI CCRYGG 1 cut(s) 891
BtsCI GGATG 4 cut(s) 766, 988, 1045, 1282
BtsI GCAGTG 1 cut(s) 1194
BtsIMutI CAGTG 3 cut(s) 1002, 1194, 1379
Cac8I GCNNGC 4 cut(s) 382, 876, 1013, 1185
CciI TCATGA 1 cut(s) 925
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 4 cut(s) 123, 228, 1240, 1336
CseI GACGC 1 cut(s) 1394
Csp6I GTAC 1 cut(s) 997
CspCI CAANNNNNGTGG 2 cut(s) 756, 791
CviAII CATG 7 cut(s) 307, 757, 808, 842, 926, 993, 1138
CviQI GTAC 1 cut(s) 997
DdeI CTNAG 5 cut(s) 53, 1067, 1087, 1207, 1435
DpnI GATC 1 cut(s) 1278
DpnII GATC 1 cut(s) 1276
DriI GACNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 182
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 182
EciI GGCGGA 1 cut(s) 658
Ecl136II GAGCTC 2 cut(s) 57, 400
Eco24I GRGCYC 2 cut(s) 59, 402
Eco47I GGWCC 4 cut(s) 123, 228, 1240, 1336
Eco53kI GAGCTC 2 cut(s) 57, 400
Eco57I CTGAAG 1 cut(s) 1452
Eco72I CACGTG 1 cut(s) 97
Eco81I CCTNAGG 1 cut(s) 1067
Eco88I CYCGRG 2 cut(s) 21, 1287
EcoICRI GAGCTC 2 cut(s) 57, 400
EcoO109I RGGNCCY 1 cut(s) 123
EcoRI GAATTC 2 cut(s) 48, 418
EcoRII CCWGG 1 cut(s) 230
EcoT22I ATGCAT 1 cut(s) 994
EcoT38I GRGCYC 2 cut(s) 59, 402
FaeI CATG 7 cut(s) 310, 760, 811, 845, 929, 996, 1141
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FaqI GGGAC 2 cut(s) 109, 696
FatI CATG 7 cut(s) 306, 756, 807, 841, 925, 992, 1137
FauI CCCGC 1 cut(s) 1162
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 5 cut(s) 944, 1302, 1319, 1322, 1389
FokI GGATG 4 cut(s) 773, 995, 1052, 1269
FriOI GRGCYC 2 cut(s) 59, 402
Fsp4HI GCNGC 5 cut(s) 944, 1302, 1319, 1322, 1389
FspBI CTAG 4 cut(s) 225, 797, 821, 1184
GlaI GCGC 1 cut(s) 240
GluI GCNGC 5 cut(s) 944, 1302, 1319, 1322, 1389
HaeIII GGCC 3 cut(s) 277, 394, 1085
HapII CCGG 2 cut(s) 497, 1405
HgaI GACGC 1 cut(s) 1394
HhaI GCGC 1 cut(s) 241
Hin1II CATG 7 cut(s) 310, 760, 811, 845, 929, 996, 1141
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HindIII AAGCTT 2 cut(s) 129, 510
HinfI GANTC 2 cut(s) 8, 700
HpaII CCGG 2 cut(s) 497, 1405
HphI GGTGA 1 cut(s) 1031
Hpy166II GTNNAC 3 cut(s) 627, 862, 1045
Hpy188I TCNGA 6 cut(s) 13, 54, 220, 433, 1210, 1471
Hpy188III TCNNGA 8 cut(s) 23, 335, 775, 926, 949, 1066, 1339, 1510
Hpy8I GTNNAC 3 cut(s) 627, 862, 1045
HpyAV CCTTC 1 cut(s) 1476
HpyCH4III ACNGT 4 cut(s) 687, 855, 1235, 1417
HpyCH4IV ACGT 2 cut(s) 96, 1162
HpyF10VI GCNNNNNNNGC 7 cut(s) 381, 599, 728, 808, 817, 1193, 1385
HpyF3I CTNAG 5 cut(s) 53, 1067, 1087, 1207, 1435
HpySE526I ACGT 2 cut(s) 96, 1162
Hsp92II CATG 7 cut(s) 310, 760, 811, 845, 929, 996, 1141
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 1 cut(s) 1276
LguI GCTCTTC 1 cut(s) 182
LmnI GCTCC 2 cut(s) 62, 110
Lsp1109I GCAGC 5 cut(s) 930, 1313, 1330, 1333, 1375
MaeI CTAG 4 cut(s) 225, 797, 821, 1184
MaeII ACGT 2 cut(s) 96, 1162
MaeIII GTNAC 6 cut(s) 266, 444, 586, 701, 835, 1537
MalI GATC 1 cut(s) 1278
MboI GATC 1 cut(s) 1276
MboII GAAGA 5 cut(s) 169, 323, 725, 1168, 1378
MflI RGATCY 1 cut(s) 1276
MhlI GDGCHC 5 cut(s) 59, 402, 804, 1137, 1208
MlsI TGGCCA 1 cut(s) 394
MluCI AATT 8 cut(s) 26, 48, 291, 301, 418, 1121, 1309, 1350
MluNI TGGCCA 1 cut(s) 394
MlyI GAGTC 2 cut(s) 17, 709
MmeI TCCRAC 1 cut(s) 84
Mox20I TGGCCA 1 cut(s) 394
Mph1103I ATGCAT 1 cut(s) 994
MroXI GAANNNNTTC 1 cut(s) 1268
MscI TGGCCA 1 cut(s) 394
MseI TTAA 8 cut(s) 654, 690, 743, 789, 813, 1308, 1454, 1478
Msp20I TGGCCA 1 cut(s) 394
MspCI CTTAAG 1 cut(s) 1477
MspI CCGG 2 cut(s) 497, 1405
MspR9I CCNGG 1 cut(s) 232
Mva1269I GAATGC 1 cut(s) 1488
MvaI CCWGG 1 cut(s) 232
MwoI GCNNNNNNNGC 7 cut(s) 381, 599, 728, 808, 817, 1193, 1385
NdeII GATC 1 cut(s) 1276
NheI GCTAGC 1 cut(s) 1183
NlaIII CATG 7 cut(s) 310, 760, 811, 845, 929, 996, 1141
NlaIV GGNNCC 5 cut(s) 106, 125, 491, 903, 1409
NmeAIII GCCGAG 2 cut(s) 521, 1222
NmuCI GTSAC 3 cut(s) 266, 701, 835
NsiI ATGCAT 1 cut(s) 994
NspI RCATGY 1 cut(s) 811
PaeR7I CTCGAG 1 cut(s) 1287
PagI TCATGA 1 cut(s) 925
PciSI GCTCTTC 1 cut(s) 182
PctI GAATGC 1 cut(s) 1488
PdmI GAANNNNTTC 1 cut(s) 1268
PkrI GCNGC 5 cut(s) 945, 1303, 1320, 1323, 1390
PleI GAGTC 2 cut(s) 16, 708
PmaCI CACGTG 1 cut(s) 97
PmlI CACGTG 1 cut(s) 97
PpsI GAGTC 2 cut(s) 16, 708
Ppu21I YACGTR 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 123
PsiI TTATAA 1 cut(s) 1549
Psp124BI GAGCTC 2 cut(s) 59, 402
Psp1406I AACGTT 1 cut(s) 1162
Psp5II RGGWCCY 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 230
PspCI CACGTG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 230
PspN4I GGNNCC 5 cut(s) 106, 125, 491, 903, 1409
PspPI GGNCC 4 cut(s) 123, 228, 1240, 1336
PspPPI RGGWCCY 1 cut(s) 123
PspXI VCTCGAGB 1 cut(s) 1287
PsuI RGATCY 1 cut(s) 1276
RsaI GTAC 1 cut(s) 998
RsaNI GTAC 1 cut(s) 997
SacI GAGCTC 2 cut(s) 59, 402
SapI GCTCTTC 1 cut(s) 182
SaqAI TTAA 8 cut(s) 654, 690, 743, 789, 813, 1308, 1454, 1478
SatI GCNGC 5 cut(s) 944, 1302, 1319, 1322, 1389
Sau3AI GATC 1 cut(s) 1276
Sau96I GGNCC 4 cut(s) 123, 228, 1240, 1336
SchI GAGTC 2 cut(s) 17, 709
ScrFI CCNGG 1 cut(s) 232
SduI GDGCHC 5 cut(s) 59, 402, 804, 1137, 1208
Sfr274I CTCGAG 1 cut(s) 1287
SinI GGWCC 4 cut(s) 123, 228, 1240, 1336
SlaI CTCGAG 1 cut(s) 1287
SmlI CTYRAG 2 cut(s) 1287, 1477
SmoI CTYRAG 2 cut(s) 1287, 1477
Sse9I AATT 8 cut(s) 26, 48, 291, 301, 418, 1121, 1309, 1350
SsiI CCGC 2 cut(s) 643, 1169
SspI AATATT 1 cut(s) 549
SspMI CTAG 4 cut(s) 225, 797, 821, 1184
SstI GAGCTC 2 cut(s) 59, 402
StyD4I CCNGG 1 cut(s) 230
TaaI ACNGT 4 cut(s) 687, 855, 1235, 1417
TaiI ACGT 2 cut(s) 99, 1165
TaqI TCGA 2 cut(s) 804, 1288
TasI AATT 8 cut(s) 26, 48, 291, 301, 418, 1121, 1309, 1350
Tru1I TTAA 8 cut(s) 654, 690, 743, 789, 813, 1308, 1454, 1478
Tru9I TTAA 8 cut(s) 654, 690, 743, 789, 813, 1308, 1454, 1478
TscAI CASTG 3 cut(s) 1009, 1194, 1386
TseFI GTSAC 3 cut(s) 266, 701, 835
TseI GCWGC 5 cut(s) 943, 1301, 1318, 1321, 1388
Tsp45I GTSAC 3 cut(s) 266, 701, 835
TspDTI ATGAA 6 cut(s) 169, 323, 858, 942, 1107, 1227
TspGWI ACGGA 4 cut(s) 60, 419, 482, 908
TspRI CASTG 3 cut(s) 1009, 1194, 1386
Vha464I CTTAAG 1 cut(s) 1477
VpaK11BI GGWCC 4 cut(s) 123, 228, 1240, 1336
XapI RAATTY 3 cut(s) 48, 418, 1350
XceI RCATGY 1 cut(s) 811
XhoI CTCGAG 1 cut(s) 1287
XmiI GTMKAC 1 cut(s) 1044
XmnI GAANNNNTTC 1 cut(s) 1268
XspI CTAG 4 cut(s) 225, 797, 821, 1184
Zsp2I ATGCAT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.