pycom04g11920

ATP-dependent zinc metalloprotease FTSH 10, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002584
Physical Location & Seq
Reverse (-)
14901600 .. 14905652
4053 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2223 bp
ATGTCGAATGTGAAGTCTGTTTTCGGAATCCCTAGAATTCGTCGCTTGTTCTCCAGCCAAGGTCCCGGGAAGAAAAATTACGAAAATTATTATCCGAAAAATAAGAAGGAAATTCCGAAAGGGGGTGAACAGAAAACGGAGTCCAAAGAGGGCTCAAGTGCAGGTGGTCAGGGGAATGCCCAGGGACAGTTTTCGAAGCAGATTCAAGATTTAATTGCTCCCTTAATGTTCATTTGCTTTATTGTCACATCATTATTCCTTAATCCTCATCAAGCGAAGGAGATAAGTTTCCAAGAATTTAAAAACAAGCTACTTGAACCTGGGTTGGTTGATCACATTGAAGTGGCAAACAAATCAGTTGCAAAAGTGTATGTGAGGAGCTCCCCACGCGAAAAAGTTCATAGTGATGATTCTGTTAGTAGTCCTGTTAAAGGTTCTCCTTCCGAAGGAAATGTAACCCAGTATAAATATTATTTTAACATTGGGAGTGTTGAATCTTTCGAAGAAAAGTTGGAGGAAGCTCAAGAAGCTTTAGGGATTGACCGTCATGATTTTGTTCCTGTAATTTATGCATCCCAAGTAATGTGGTTGCAAGAGTTGTTAAGGTATGGGCCAACAGTATTGCTTCTGGGAGCCCTTTGGTTCATGAGCCGAAAAATGCCTAACATTGGTGGTCCAGGTGGAACAGGTGGCCGAGGCATATTCAACATAGGTAAAGCACAGATCACGAAGTTGGACAAGAATGCCAAAAACAAGGTTTACTTTAAAGATGTAGCTGGCTGTGATGAGGCTAAGCAAGAAATAATGGAGTTTGTGCACTTTCTAAAAAATCCTAAGAAATATGAGGAATTGGGAGCAAAAATTCCAAAAGGTGCTCTTCTTGTTGGCCCTCCTGGAACAGGAAAGACGCTTCTTGCAAAGGCAACTGCTGGTGAATCTGGGGTGCCTTTTCTATCTATATCTGGGTCAGATTTTATGGAAATGTTTGTTGGTGTTGGTCCGTCAAGGGTTCGAAGCTTATTTCAAGAGGCAAGACAGTGCGCACCCAGTATAATATTTATTGATGAGATCGATGCAATTGGCCGGGCAAGAGGGCGTGGAGGCTTTTCAGGTGGCCATGACGAGCGTGAAAGTACTCTCAATCAATTGCTTGTAGAAATGGATGGATTTGGAACCACTGCTGGAGTTGTTGTACTTGCTGGCACTAATAGGCCTGATATTCTGGATAAAGCTCTCTTGAGGCCAGGCCGGTTTGACCGTCAAATTACTATTGATAAACCAGACATCAAGGGTCGTAACCAGATTTTTCAAATATATCTGAGCAAGCTCAAGCTGGATCTGGAACCATCATTTTACTCTGAGAGGCTTGCTGCTCTCACTCCTGGATTTGCTGGAGCAGACATAGCAAACGTCTGCAATGAAGCTGCCTTGATTGCTGCAAGAAATGAGAGTCCAAAGATCACCATGAAACATTTTGAAGCGGCTATAGACAGGGTGATAGGCGGCCTAGAAAAGAAGAACAAGGTTATAAGCAAGCTGGAAAGACGGACTGTTGCTTACCATGAATCTGGTCATGCTGTTGCGGGTTGGTTCCTGGAACACGCAGAACCTTTGCTTAAAGTAACTATTGTTCCGCGTGGCACTGCAGCACTGGGGTTTGCTCAGTATGTGCCCAATGAAAATCTTCTTATGACCAAAGAGCAGCTTTTTGATATGACATGCATGACCCTTGGTGGCCGAGCTGCTGAGCAGGTTTTGTTGGGGAAGATTTCAACTGGAGCGCAGAACGACTTGGAGAAAGTGACCAAAATGACTTATGCTCAAGTTGCAGTGTATGGCTTCAGTGACAAGGTAGGTCTTCTTTCGTTTCCTCAGAGGGAGGATTCATTTGAGATGACCAAGCCATACAGTAGCAAAACTGGTGCAATAATTGACAGTGAAGTTAGAGAATGGGTGGGTAAAGCATACTTCCGCACTGTCCAGTTGATAGAGGAGCACAAAGAGCATGTAGGACAGATTGCAGAGCTGTTGCTGGAAAAGGAAGTCCTTCACCAAGAGGATTTGATTCGAGTACTGGGAGAACGCCCATTCAAGTCGAATGAGCCCACAAACTATGACAGATTCAAGGAAGGGTTCCAAGAAGAAGATAAGGAGGCTAAAGAGACCAATGCGAAGGATGGTCGGTCGCCACCCATTCAACCGGATGTCGTTGTGCCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FtsH_ext Peptidase_M41

Protein Analysis

741

Amino Acids

81.81

Weight (kDa)

7.99

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsH_ext PF06480 75 - 182 9.2e-10 FtsH Extracellular
AAA PF00004 293 - 425 9.6e-43 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 454 - 494 2e-13 AAA+ lid domain
Peptidase_M41 PF01434 509 - 690 1.1e-64 Peptidase family M41
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1529
AarI CACCTGC 1 cut(s) 152
Acc16I TGCGCA 1 cut(s) 1042
Acc36I ACCTGC 2 cut(s) 152, 1741
AccB1I GGYRCC 1 cut(s) 943
AccII CGCG 2 cut(s) 390, 1636
AciI CCGC 5 cut(s) 1481, 1503, 1583, 1634, 1972
AclWI GGATC 1 cut(s) 1344
AcoI YGGCCR 4 cut(s) 691, 1081, 1114, 1735
AcsI RAATTY 4 cut(s) 36, 111, 296, 861
AcuI CTGAAG 1 cut(s) 1825
AfaI GTAC 3 cut(s) 1135, 1194, 2073
AfiI CCNNNNNNNGG 5 cut(s) 122, 431, 446, 668, 899
AjnI CCWGG 7 cut(s) 180, 319, 676, 892, 1243, 1381, 1593
AjuI GAANNNNNNNTTGG 2 cut(s) 972, 1004
Alw21I GWGCWC 4 cut(s) 383, 819, 877, 1998
Alw26I GTCTC 1 cut(s) 2156
Alw44I GTGCAC 1 cut(s) 815
AlwI GGATC 1 cut(s) 1344
Ama87I CYCGRG 1 cut(s) 65
ApaLI GTGCAC 1 cut(s) 815
ApeKI GCWGC 6 cut(s) 1370, 1424, 1436, 1646, 1702, 1742
ApoI RAATTY 4 cut(s) 36, 111, 296, 861
ArsI GACNNNNNNTTYG 1 cut(s) 31
Asp700I GAANNNNTTC 3 cut(s) 396, 1683, 2046
AspLEI GCGC 2 cut(s) 1043, 1783
AspS9I GGNCC 5 cut(s) 62, 611, 674, 887, 998
AsuC2I CCSGG 3 cut(s) 66, 67, 1085
AsuHPI GGTGA 5 cut(s) 137, 944, 1453, 1507, 2042
AsuII TTCGAA 3 cut(s) 194, 501, 1012
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 3 cut(s) 62, 674, 998
BaeGI GKGCMC 2 cut(s) 819, 1674
BalI TGGCCA 1 cut(s) 1116
BanI GGYRCC 1 cut(s) 943
BanII GRGCYC 4 cut(s) 155, 383, 637, 2106
BarI GAAGNNNNNNTAC 2 cut(s) 62, 94
BbsI GAAGAC 1 cut(s) 1850
Bbv12I GWGCWC 4 cut(s) 383, 819, 877, 1998
BbvI GCAGC 6 cut(s) 1357, 1411, 1423, 1658, 1714, 1729
BccI CCATC 3 cut(s) 1157, 1354, 2171
BciT130I CCWGG 7 cut(s) 182, 321, 678, 894, 1245, 1383, 1595
BclI TGATCA 1 cut(s) 331
BcnI CCSGG 3 cut(s) 66, 67, 1085
BcoDI GTCTC 1 cut(s) 2156
BfaI CTAG 2 cut(s) 33, 1508
BfmI CTRYAG 2 cut(s) 1485, 1644
BfuAI ACCTGC 2 cut(s) 152, 1741
BisI GCNGC 8 cut(s) 1371, 1425, 1437, 1482, 1504, 1647, 1703, 1743
BlpI GCTNAGC 2 cut(s) 792, 1746
BlsI GCNGC 8 cut(s) 1372, 1426, 1438, 1483, 1505, 1648, 1704, 1744
BmcAI AGTACT 2 cut(s) 1135, 2073
Bme18I GGWCC 3 cut(s) 62, 674, 998
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 5 cut(s) 62, 611, 674, 887, 998
BmiI GGNNCC 7 cut(s) 64, 634, 945, 1174, 1344, 1592, 2135
BmrI ACTGGG 4 cut(s) 454, 1041, 1661, 2084
BmsI GCATC 2 cut(s) 581, 1063
BmuI ACTGGG 4 cut(s) 454, 1041, 1661, 2084
BpiI GAAGAC 1 cut(s) 1850
BpmI CTGGAG 4 cut(s) 37, 1203, 1413, 1797
Bpu1102I GCTNAGC 2 cut(s) 792, 1746
Bpu14I TTCGAA 3 cut(s) 194, 501, 1012
BpuEI CTTGAG 5 cut(s) 139, 507, 1258, 1313, 1806
BpuMI CCSGG 3 cut(s) 66, 67, 1085
Bsa29I ATCGAT 1 cut(s) 1071
BsaI GGTCTC 1 cut(s) 2156
BsaJI CCNNGG 7 cut(s) 58, 65, 180, 181, 320, 694, 1729
BsaWI WCCGGW 1 cut(s) 2200
BsaXI ACNNNNNCTCC 2 cut(s) 1176, 1206
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 431, 446, 668, 899
Bse118I RCCGGY 1 cut(s) 1248
Bse1I ACTGG 7 cut(s) 460, 1047, 1656, 1780, 1924, 1981, 2079
Bse3DI GCAATG 1 cut(s) 1423
BseBI CCWGG 7 cut(s) 182, 321, 678, 894, 1245, 1383, 1595
BseCI ATCGAT 1 cut(s) 1071
BseDI CCNNGG 7 cut(s) 58, 65, 180, 181, 320, 694, 1729
BseGI GGATG 4 cut(s) 572, 1168, 2182, 2209
BseLI CCNNNNNNNGG 5 cut(s) 122, 431, 446, 668, 899
BseMI GCAATG 1 cut(s) 1423
BseMII CTCAG 5 cut(s) 1310, 1350, 1676, 1737, 1886
BseNI ACTGG 7 cut(s) 460, 1047, 1656, 1780, 1924, 1981, 2079
BseRI GAGGAG 2 cut(s) 391, 2006
BseSI GKGCMC 2 cut(s) 819, 1674
BseXI GCAGC 6 cut(s) 1357, 1411, 1423, 1658, 1714, 1729
BsgI GTGCAG 1 cut(s) 180
Bsh1236I CGCG 2 cut(s) 390, 1636
Bsh1285I CGRYCG 1 cut(s) 2186
BshNI GGYRCC 1 cut(s) 943
BshVI ATCGAT 1 cut(s) 1071
BsiEI CGRYCG 1 cut(s) 2186
BsiHKAI GWGCWC 4 cut(s) 383, 819, 877, 1998
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 4 cut(s) 66, 1084, 1249, 2201
BslFI GGGAC 2 cut(s) 48, 198
BslI CCNNNNNNNGG 5 cut(s) 122, 431, 446, 668, 899
BsmAI GTCTC 1 cut(s) 2156
BsmFI GGGAC 2 cut(s) 48, 198
BsmI GAATGC 2 cut(s) 181, 748
Bso31I GGTCTC 1 cut(s) 2156
BsoBI CYCGRG 1 cut(s) 65
Bsp119I TTCGAA 3 cut(s) 194, 501, 1012
Bsp1286I GDGCHC 8 cut(s) 155, 383, 637, 819, 877, 1674, 1998, 2106
Bsp143I GATC 5 cut(s) 331, 723, 1068, 1336, 1458
Bsp1720I GCTNAGC 2 cut(s) 792, 1746
BspACI CCGC 5 cut(s) 1481, 1503, 1583, 1634, 1972
BspCNI CTCAG 5 cut(s) 1311, 1351, 1675, 1738, 1885
BspDI ATCGAT 1 cut(s) 1071
BspFNI CGCG 2 cut(s) 390, 1636
BspHI TCATGA 2 cut(s) 547, 645
BspLI GGNNCC 7 cut(s) 64, 634, 945, 1174, 1344, 1592, 2135
BspMAI CTGCAG 1 cut(s) 1648
BspMI ACCTGC 2 cut(s) 152, 1741
BspPI GGATC 1 cut(s) 1344
BspQI GCTCTTC 1 cut(s) 882
BspT104I TTCGAA 3 cut(s) 194, 501, 1012
BspT107I GGYRCC 1 cut(s) 943
BspTNI GGTCTC 1 cut(s) 2156
BsrDI GCAATG 1 cut(s) 1423
BsrFI RCCGGY 1 cut(s) 1248
BsrI ACTGG 7 cut(s) 460, 1047, 1656, 1780, 1924, 1981, 2079
BssAI RCCGGY 1 cut(s) 1248
BssECI CCNNGG 7 cut(s) 58, 65, 180, 181, 320, 694, 1729
BssMI GATC 5 cut(s) 331, 723, 1068, 1336, 1458
BssT1I CCWWGG 2 cut(s) 58, 1729
Bst2UI CCWGG 7 cut(s) 182, 321, 678, 894, 1245, 1383, 1595
Bst4CI ACNGT 9 cut(s) 189, 545, 619, 1038, 1259, 1552, 1910, 1937, 1978
Bst6I CTCTTC 1 cut(s) 882
BstBI TTCGAA 3 cut(s) 194, 501, 1012
BstC8I GCNNGC 6 cut(s) 778, 1201, 1325, 1368, 1535, 2217
BstDEI CTNAG 7 cut(s) 792, 834, 1319, 1359, 1662, 1746, 1872
BstENI CCTNNNNNAGG 3 cut(s) 429, 444, 897
BstF5I GGATG 4 cut(s) 572, 1168, 2182, 2209
BstFNI CGCG 2 cut(s) 390, 1636
BstHHI GCGC 2 cut(s) 1043, 1783
BstKTI GATC 5 cut(s) 334, 726, 1071, 1339, 1461
BstMAI GTCTC 1 cut(s) 2156
BstMBI GATC 5 cut(s) 331, 723, 1068, 1336, 1458
BstMCI CGRYCG 1 cut(s) 2186
BstMWI GCNNNNNNNGC 6 cut(s) 387, 527, 1403, 1433, 1826, 2002
BstNI CCWGG 7 cut(s) 182, 321, 678, 894, 1245, 1383, 1595
BstNSI RCATGY 2 cut(s) 1722, 2009
BstSFI CTRYAG 2 cut(s) 1485, 1644
BstSLI GKGCMC 2 cut(s) 819, 1674
BstUI CGCG 2 cut(s) 390, 1636
BstV1I GCAGC 6 cut(s) 1357, 1411, 1423, 1658, 1714, 1729
BstV2I GAAGAC 1 cut(s) 1850
BstX2I RGATCY 1 cut(s) 1336
BstXI CCANNNNNNTGG 1 cut(s) 1568
BstYI RGATCY 1 cut(s) 1336
Bsu15I ATCGAT 1 cut(s) 1071
BsuTUI ATCGAT 1 cut(s) 1071
BtsCI GGATG 4 cut(s) 572, 1168, 2182, 2209
BtsI GCAGTG 3 cut(s) 1176, 1641, 1836
BtsIMutI CAGTG 8 cut(s) 1043, 1176, 1641, 1649, 1836, 1849, 1942, 1974
BveI ACCTGC 2 cut(s) 152, 1741
Cac8I GCNNGC 6 cut(s) 778, 1201, 1325, 1368, 1535, 2217
CciI TCATGA 2 cut(s) 547, 645
CfoI GCGC 2 cut(s) 1043, 1783
Cfr10I RCCGGY 1 cut(s) 1248
Cfr13I GGNCC 5 cut(s) 62, 611, 674, 887, 998
Cfr9I CCCGGG 1 cut(s) 65
ClaI ATCGAT 1 cut(s) 1071
CseI GACGC 1 cut(s) 916
Csp6I GTAC 3 cut(s) 1134, 1193, 2072
CspCI CAANNNNNGTGG 2 cut(s) 566, 601
CviAII CATG 9 cut(s) 548, 646, 1118, 1465, 1562, 1574, 1719, 1723, 2006
CviQI GTAC 3 cut(s) 1134, 1193, 2072
DdeI CTNAG 7 cut(s) 792, 834, 1319, 1359, 1662, 1746, 1872
DpnI GATC 5 cut(s) 333, 725, 1070, 1338, 1460
DpnII GATC 5 cut(s) 331, 723, 1068, 1336, 1458
DraI TTTAAA 2 cut(s) 301, 766
EaeI YGGCCR 4 cut(s) 691, 1081, 1114, 1735
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
Ecl136II GAGCTC 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 58, 1729
Eco147I AGGCCT 1 cut(s) 1213
Eco24I GRGCYC 4 cut(s) 155, 383, 637, 2106
Eco31I GGTCTC 1 cut(s) 2156
Eco47I GGWCC 3 cut(s) 62, 674, 998
Eco53kI GAGCTC 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 1825
Eco88I CYCGRG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 381
EcoNI CCTNNNNNAGG 3 cut(s) 429, 444, 897
EcoO109I RGGNCCY 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 36
EcoRII CCWGG 7 cut(s) 180, 319, 676, 892, 1243, 1381, 1593
EcoT14I CCWWGG 2 cut(s) 58, 1729
EcoT22I ATGCAT 2 cut(s) 574, 1724
EcoT38I GRGCYC 4 cut(s) 155, 383, 637, 2106
ErhI CCWWGG 2 cut(s) 58, 1729
FaeI CATG 9 cut(s) 551, 649, 1121, 1468, 1565, 1577, 1722, 1726, 2009
FalI AAGNNNNNCTT 6 cut(s) 746, 778, 861, 893, 1689, 1721
FaqI GGGAC 2 cut(s) 48, 198
FatI CATG 9 cut(s) 547, 645, 1117, 1464, 1561, 1573, 1718, 1722, 2005
FauI CCCGC 1 cut(s) 1576
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 8 cut(s) 1371, 1425, 1437, 1482, 1504, 1647, 1703, 1743
FokI GGATG 4 cut(s) 559, 1175, 2189, 2216
FriOI GRGCYC 4 cut(s) 155, 383, 637, 2106
Fsp4HI GCNGC 8 cut(s) 1371, 1425, 1437, 1482, 1504, 1647, 1703, 1743
FspAI RTGCGCAY 1 cut(s) 1042
FspBI CTAG 2 cut(s) 33, 1508
FspI TGCGCA 1 cut(s) 1042
GlaI GCGC 2 cut(s) 1042, 1782
GluI GCNGC 8 cut(s) 1371, 1425, 1437, 1482, 1504, 1647, 1703, 1743
GsuI CTGGAG 4 cut(s) 37, 1203, 1413, 1797
HapII CCGG 4 cut(s) 66, 1084, 1249, 2201
HgaI GACGC 1 cut(s) 916
HhaI GCGC 2 cut(s) 1043, 1783
Hin1II CATG 9 cut(s) 551, 649, 1121, 1468, 1565, 1577, 1722, 1726, 2009
Hin6I GCGC 2 cut(s) 1041, 1781
HinP1I GCGC 2 cut(s) 1041, 1781
HindIII AAGCTT 2 cut(s) 528, 1015
HpaII CCGG 4 cut(s) 66, 1084, 1249, 2201
HphI GGTGA 5 cut(s) 137, 944, 1453, 1507, 2042
Hpy166II GTNNAC 3 cut(s) 128, 760, 817
Hpy188I TCNGA 8 cut(s) 26, 96, 117, 445, 970, 1320, 1360, 1875
Hpy188III TCNNGA 9 cut(s) 206, 524, 548, 646, 727, 1025, 1223, 1237, 1340
Hpy8I GTNNAC 3 cut(s) 128, 760, 817
Hpy99I CGWCG 1 cut(s) 45
HpyAV CCTTC 7 cut(s) 100, 271, 440, 450, 2057, 2123, 2167
HpyCH4III ACNGT 9 cut(s) 189, 545, 619, 1038, 1259, 1552, 1910, 1937, 1978
HpyCH4IV ACGT 1 cut(s) 1410
HpyF10VI GCNNNNNNNGC 6 cut(s) 387, 527, 1403, 1433, 1826, 2002
HpyF3I CTNAG 7 cut(s) 792, 834, 1319, 1359, 1662, 1746, 1872
HpySE526I ACGT 1 cut(s) 1410
Hsp92II CATG 9 cut(s) 551, 649, 1121, 1468, 1565, 1577, 1722, 1726, 2009
HspAI GCGC 2 cut(s) 1041, 1781
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 5 cut(s) 331, 723, 1068, 1336, 1458
LguI GCTCTTC 1 cut(s) 882
LmnI GCTCC 8 cut(s) 223, 378, 386, 632, 854, 1394, 1778, 1993
Lsp1109I GCAGC 6 cut(s) 1357, 1411, 1423, 1658, 1714, 1729
LweI GCATC 2 cut(s) 581, 1063
MaeI CTAG 2 cut(s) 33, 1508
MaeII ACGT 1 cut(s) 1410
MaeIII GTNAC 6 cut(s) 244, 454, 1295, 1621, 1801, 1844
MalI GATC 5 cut(s) 333, 725, 1070, 1338, 1460
MboI GATC 5 cut(s) 331, 723, 1068, 1336, 1458
MboII GAAGA 9 cut(s) 82, 515, 869, 1528, 1676, 1777, 1850, 2153, 2156
MfeI CAATTG 2 cut(s) 1077, 1145
MflI RGATCY 1 cut(s) 1336
MhlI GDGCHC 8 cut(s) 155, 383, 637, 819, 877, 1674, 1998, 2106
MlsI TGGCCA 1 cut(s) 1116
MluNI TGGCCA 1 cut(s) 1116
MlyI GAGTC 2 cut(s) 149, 1459
MmeI TCCRAC 2 cut(s) 492, 714
Mox20I TGGCCA 1 cut(s) 1116
Mph1103I ATGCAT 2 cut(s) 574, 1724
MroXI GAANNNNTTC 3 cut(s) 396, 1683, 2046
MscI TGGCCA 1 cut(s) 1116
MseI TTAA 9 cut(s) 212, 224, 261, 300, 429, 477, 602, 765, 1617
MslI CAYNNNNRTG 2 cut(s) 341, 405
Msp20I TGGCCA 1 cut(s) 1116
MspI CCGG 4 cut(s) 66, 1084, 1249, 2201
MunI CAATTG 2 cut(s) 1077, 1145
Mva1269I GAATGC 2 cut(s) 181, 748
MvaI CCWGG 7 cut(s) 182, 321, 678, 894, 1245, 1383, 1595
MvnI CGCG 2 cut(s) 390, 1636
MwoI GCNNNNNNNGC 6 cut(s) 387, 527, 1403, 1433, 1826, 2002
NciI CCSGG 3 cut(s) 66, 67, 1085
NdeII GATC 5 cut(s) 331, 723, 1068, 1336, 1458
NlaIII CATG 9 cut(s) 551, 649, 1121, 1468, 1565, 1577, 1722, 1726, 2009
NlaIV GGNNCC 7 cut(s) 64, 634, 945, 1174, 1344, 1592, 2135
NmeAIII GCCGAG 2 cut(s) 719, 1763
NmuCI GTSAC 3 cut(s) 244, 1801, 1844
NsbI TGCGCA 1 cut(s) 1042
NsiI ATGCAT 2 cut(s) 574, 1724
NspI RCATGY 2 cut(s) 1722, 2009
NspV TTCGAA 3 cut(s) 194, 501, 1012
PagI TCATGA 2 cut(s) 547, 645
PaqCI CACCTGC 1 cut(s) 152
PasI CCCWGGG 1 cut(s) 181
PceI AGGCCT 1 cut(s) 1213
PciSI GCTCTTC 1 cut(s) 882
PctI GAATGC 2 cut(s) 181, 748
PdmI GAANNNNTTC 3 cut(s) 396, 1683, 2046
PfeI GAWTC 9 cut(s) 27, 202, 410, 494, 935, 1565, 1883, 2065, 2121
PfoI TCCNGGA 3 cut(s) 892, 1381, 1593
PkrI GCNGC 8 cut(s) 1372, 1426, 1438, 1483, 1505, 1648, 1704, 1744
PleI GAGTC 2 cut(s) 148, 1458
PpsI GAGTC 2 cut(s) 148, 1458
PpuMI RGGWCCY 1 cut(s) 62
PsiI TTATAA 1 cut(s) 1529
Psp124BI GAGCTC 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 62
Psp6I CCWGG 7 cut(s) 180, 319, 676, 892, 1243, 1381, 1593
PspGI CCWGG 7 cut(s) 180, 319, 676, 892, 1243, 1381, 1593
PspN4I GGNNCC 7 cut(s) 64, 634, 945, 1174, 1344, 1592, 2135
PspPI GGNCC 5 cut(s) 62, 611, 674, 887, 998
PspPPI RGGWCCY 1 cut(s) 62
PsrI GAACNNNNNNTAC 2 cut(s) 1614, 1646
PstI CTGCAG 1 cut(s) 1648
PsuI RGATCY 1 cut(s) 1336
RsaI GTAC 3 cut(s) 1135, 1194, 2073
RsaNI GTAC 3 cut(s) 1134, 1193, 2072
RseI CAYNNNNRTG 2 cut(s) 341, 405
SacI GAGCTC 1 cut(s) 383
SapI GCTCTTC 1 cut(s) 882
SaqAI TTAA 9 cut(s) 212, 224, 261, 300, 429, 477, 602, 765, 1617
SatI GCNGC 8 cut(s) 1371, 1425, 1437, 1482, 1504, 1647, 1703, 1743
Sau3AI GATC 5 cut(s) 331, 723, 1068, 1336, 1458
Sau96I GGNCC 5 cut(s) 62, 611, 674, 887, 998
ScaI AGTACT 2 cut(s) 1135, 2073
SchI GAGTC 2 cut(s) 149, 1459
SduI GDGCHC 8 cut(s) 155, 383, 637, 819, 877, 1674, 1998, 2106
SfaNI GCATC 2 cut(s) 581, 1063
SfcI CTRYAG 2 cut(s) 1485, 1644
SfuI TTCGAA 3 cut(s) 194, 501, 1012
SinI GGWCC 3 cut(s) 62, 674, 998
SmaI CCCGGG 1 cut(s) 67
SmiMI CAYNNNNRTG 2 cut(s) 341, 405
SmlI CTYRAG 5 cut(s) 154, 522, 1237, 1328, 1821
SmoI CTYRAG 5 cut(s) 154, 522, 1237, 1328, 1821
SseBI AGGCCT 1 cut(s) 1213
SsiI CCGC 5 cut(s) 1481, 1503, 1583, 1634, 1972
SspI AATATT 2 cut(s) 470, 1056
SspMI CTAG 2 cut(s) 33, 1508
SstI GAGCTC 1 cut(s) 383
StuI AGGCCT 1 cut(s) 1213
StyI CCWWGG 2 cut(s) 58, 1729
TaaI ACNGT 9 cut(s) 189, 545, 619, 1038, 1259, 1552, 1910, 1937, 1978
TaiI ACGT 1 cut(s) 1413
TaqI TCGA 7 cut(s) 5, 194, 501, 1012, 1071, 2068, 2096
TaqII GACCGA 1 cut(s) 2172
TatI WGTACW 3 cut(s) 1133, 1192, 2071
TauI GCSGC 2 cut(s) 1484, 1506
TfiI GAWTC 9 cut(s) 27, 202, 410, 494, 935, 1565, 1883, 2065, 2121
Tru1I TTAA 9 cut(s) 212, 224, 261, 300, 429, 477, 602, 765, 1617
Tru9I TTAA 9 cut(s) 212, 224, 261, 300, 429, 477, 602, 765, 1617
TscAI CASTG 8 cut(s) 1043, 1183, 1648, 1656, 1836, 1849, 1942, 1981
TseFI GTSAC 3 cut(s) 244, 1801, 1844
TseI GCWGC 6 cut(s) 1370, 1424, 1436, 1646, 1702, 1742
Tsp45I GTSAC 3 cut(s) 244, 1801, 1844
TspDTI ATGAA 8 cut(s) 220, 389, 634, 1434, 1481, 1578, 1692, 1875
TspGWI ACGGA 3 cut(s) 152, 990, 1561
TspMI CCCGGG 1 cut(s) 65
TspRI CASTG 8 cut(s) 1043, 1183, 1648, 1656, 1836, 1849, 1942, 1981
VneI GTGCAC 1 cut(s) 815
VpaK11BI GGWCC 3 cut(s) 62, 674, 998
XagI CCTNNNNNAGG 3 cut(s) 429, 444, 897
XapI RAATTY 4 cut(s) 36, 111, 296, 861
XceI RCATGY 2 cut(s) 1722, 2009
XmaI CCCGGG 1 cut(s) 65
XmnI GAANNNNTTC 3 cut(s) 396, 1683, 2046
XspI CTAG 2 cut(s) 33, 1508
ZrmI AGTACT 2 cut(s) 1135, 2073
Zsp2I ATGCAT 2 cut(s) 574, 1724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.