Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Forward (+)

17195650 .. 17198147

Gene Structure 2498 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1815 bp

ATGGACACCAAAATCGGTTCCCTCGACGTTTGCAAGCCGGCGTGCACCGACGTCGGCAGCCTCCCAAACGGCGCCGCTTCGGCAATCCAAAGCACTGCCCCCTCCACCGTCAATTCCTCTGACGCCACTCTGGGTCGCCACATCGCCCGCCGACTCGTCCAAATCGGCGTCACTGACGTGTTCACTGTCCCCGGTGACTTTAACTTGACCCTCCTCGACCACCTCATCGCCGAGCCCGGGCTCACCAACATCGGCTGCTGCAACGAACTCAACGCCGGGTACGCTGCTGACGGCTACGCTCGGTCGCGGGGAGTCGGGGCGTGTGCTGTTACTTTCACTGTGGGTGGGCTCAGTGTTCTCAATGCTATCGCCGGAGCTTACAGTGAGAATCTGCCATTGATTTGTATAGTTGGAGGACCCAACTCGATCGATCACGGGACGCACAGGATTCTTCACCACACTATTGGGTTACCGGATTTTAGCCAAGAGCTGAGATGCTTCCAGACCGTGACTTGCTATCAGGCTGTGGTAAATAATCTGGAAGATGCTCATGAAATGATTGATACCGCAATTTCAACCGCCTTGAAAGAAAGCAAGCCTGTTTATATCAGCATAAGCTGCAACTTGGCTGGAATTCCTCATCCTACTTTTAGCCGGGATCCTGTTCCGTTCTCATTGTCTCCAAGATTGAGCAATCATTTGGGCTTAGAGGCTGCCGTGGAGGCGGCTGCAGAGTTCTTGAACAAGGCAGTGAAGCCGGTTATGGTAGGGGGGCCTAAACTTCGAGTTGCACATGCTGGCAATGCCTTTGTTGAACTAGCTGATGCTAGTGGTTATGCTCTTGCTGTCATGCCATCTGCAAAGGGCCTTGTGCCAGAGCACCACCCCCATTTCATTGGAACATACTGGGGTGCTGTGAGCACTGCCTTTTGCGCCGAGATTGTGGAGTCTGCAGATGCATACTTGTTTGCTGGACCGATTTTCAACGACTACAGCTCCGTTGGATACTCTCTGCTTCTCAAGAAAGAGAAGGCAATTGTGGTGCAGCCCGATCGCGTGACGATAGGAAATGGCCCTTCATTTGGTTGTGTTCTGATGAAGGATTTCCTCCGAGCTCTGGCAAAGAGGCTCAAGCACAACAAAACTGCTCATGAGAACTACAGCAGGATCTTTGTTCCCAATGGACACCCTCTAAAGTCTGCACCGAAAGAACCTTTGAGGGTTAATGTTCTGTTCCAACACATCCAGAATTTGCTGTCAAGTGAAACTGCTGTGATTGCTGAGACAGGGGACTCATGGTTTAACTGCCAGAAACTGAAATTGCCGGCTGGTTGCGGGTATGAGTTCCAGATGCAGTATGGATCAATTGGTTGGTCAGTTGGAGCAACTCTTGGGTATGCTCAAGCTGTTCCTGAGAAGCGTGTGATTGCTTTCATCGGCGATGGGAGTTTCCAGGTGACAGTTCAAGATGTGTCCACCATGATCCGAAATGGGCAGAAGAACATCATCTTCCTGATAAACAACGGCGGATACACAATTGAGGTGGAGATCCATGACGGACCCTACAATGTGATTAAGAACTGGAACTACACTGGACTAGTTGATGCCATCCACAACGGGGAGGGCAAGTGCTGGACAACCAAGGTCCGTTGCGAAGAGGAGCTGATTGAAGCGATTGAGACTGCAACCGGGGCGAAGAAGGATAGCTTGTGCTTCATTGAGGTGATAGCCCACAAGGACGATACCAGCAAAGAGTTGCTTGAGTGGGGGTCTAGGGTTTCTGCTGCCAACAGCCGCCCACCCAACCCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

605

Amino Acids

64.82

Weight (kDa)

6.06

Isoelectric Point (pI)

32.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	43 - 148	2.1e-29	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	240 - 359	9.3e-23	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	451 - 575	8.2e-16	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	54
AccB1I	GGYRCC	1 cut(s)	71
AccII	CGCG	2 cut(s)	307, 1056
AciI	CCGC	9 cut(s)	75, 148, 307, 567, 579, 725, 1335, 1527, 1795
AclWI	GGATC	6 cut(s)	653, 666, 1175, 1369, 1477, 1543
AcsI	RAATTY	2 cut(s)	633, 1249
AcyI	GRCGYC	4 cut(s)	51, 72, 123, 168
AfaI	GTAC	1 cut(s)	281
AfiI	CCNNNNNNNGG	4 cut(s)	237, 1082, 1117, 1618
AflIII	ACRYGT	1 cut(s)	177
AgsI	TTSAA	7 cut(s)	576, 586, 742, 815, 985, 1466, 1670
AhlI	ACTAGT	1 cut(s)	1597
AjiI	CACGTC	1 cut(s)	178
AjnI	CCWGG	1 cut(s)	1452
AleI	CACNNNNGTG	1 cut(s)	176
AluBI	AGCT	9 cut(s)	377, 490, 618, 821, 996, 1115, 1406, 1663, 1707
AluI	AGCT	9 cut(s)	377, 490, 618, 821, 996, 1115, 1406, 1663, 1707
Alw21I	GWGCWC	4 cut(s)	47, 882, 923, 1117
Alw26I	GTCTC	3 cut(s)	684, 1277, 1673
Alw44I	GTGCAC	1 cut(s)	43
AlwI	GGATC	6 cut(s)	653, 666, 1175, 1369, 1477, 1543
AlwNI	CAGNNNCTG	1 cut(s)	1315
Ama87I	CYCGRG	1 cut(s)	236
AoxI	GGCC	3 cut(s)	773, 865, 1072
ApaLI	GTGCAC	1 cut(s)	43
ApeKI	GCWGC	9 cut(s)	57, 255, 258, 284, 618, 713, 728, 1045, 1784
ApoI	RAATTY	2 cut(s)	633, 1249
Asp700I	GAANNNNTTC	1 cut(s)	1103
AspLEI	GCGC	2 cut(s)	74, 935
AspS9I	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
AsuC2I	CCSGG	6 cut(s)	192, 237, 238, 277, 656, 1690
AsuHPI	GGTGA	5 cut(s)	206, 235, 446, 1468, 1735
AvaI	CYCGRG	1 cut(s)	236
AvaII	GGWCC	4 cut(s)	416, 974, 1559, 1645
BaeGI	GKGCMC	1 cut(s)	47
BamHI	GGATCC	1 cut(s)	658
BanI	GGYRCC	1 cut(s)	71
BanII	GRGCYC	4 cut(s)	237, 243, 351, 1117
Bbv12I	GWGCWC	4 cut(s)	47, 882, 923, 1117
BbvI	GCAGC	9 cut(s)	69, 242, 245, 271, 605, 700, 715, 1057, 1771
BccI	CCATC	3 cut(s)	862, 1436, 1616
BceAI	ACGGC	4 cut(s)	85, 307, 701, 1540
BcgI	CGANNNNNNTGC	4 cut(s)	34, 68, 1034, 1068
BciT130I	CCWGG	1 cut(s)	1454
BciVI	GTATCC	2 cut(s)	998, 1523
BcnI	CCSGG	6 cut(s)	192, 237, 238, 277, 656, 1690
BcoDI	GTCTC	3 cut(s)	684, 1277, 1673
BcuI	ACTAGT	1 cut(s)	1597
BfaI	CTAG	4 cut(s)	818, 828, 1598, 1773
BfmI	CTRYAG	4 cut(s)	729, 951, 991, 1159
BfoI	RGCGCY	1 cut(s)	75
BfuI	GTATCC	2 cut(s)	998, 1523
BglI	GCCNNNNNGGC	2 cut(s)	80, 722
Bme1390I	CCNGG	7 cut(s)	192, 237, 238, 277, 656, 1454, 1690
Bme18I	GGWCC	4 cut(s)	416, 974, 1559, 1645
BmeT110I	CYCGRG	1 cut(s)	236
BmgBI	CACGTC	1 cut(s)	178
BmgT120I	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
BmiI	GGNNCC	6 cut(s)	19, 73, 418, 660, 774, 1561
BmrFI	CCNGG	7 cut(s)	192, 237, 238, 277, 656, 1454, 1690
BmrI	ACTGGG	1 cut(s)	916
BmsI	GCATC	6 cut(s)	485, 535, 814, 946, 1341, 1594
BmuI	ACTGGG	1 cut(s)	916
BpuEI	CTTGAG	4 cut(s)	1004, 1115, 1386, 1781
BpuMI	CCSGG	6 cut(s)	192, 237, 238, 277, 656, 1690
Bsa29I	ATCGAT	1 cut(s)	429
BsaHI	GRCGYC	4 cut(s)	51, 72, 123, 168
BsaJI	CCNNGG	5 cut(s)	190, 236, 717, 1641, 1689
BsaWI	WCCGGW	1 cut(s)	472
BsaXI	ACNNNNNCTCC	4 cut(s)	980, 1010, 1613, 1643
Bsc4I	CCNNNNNNNGG	4 cut(s)	237, 1082, 1117, 1618
Bse118I	RCCGGY	3 cut(s)	37, 757, 1324
Bse1I	ACTGG	3 cut(s)	911, 1586, 1597
Bse3DI	GCAATG	1 cut(s)	808
BseBI	CCWGG	1 cut(s)	1454
BseCI	ATCGAT	1 cut(s)	429
BseDI	CCNNGG	5 cut(s)	190, 236, 717, 1641, 1689
BseGI	GGATG	3 cut(s)	640, 1242, 1608
BseLI	CCNNNNNNNGG	4 cut(s)	237, 1082, 1117, 1618
BseMI	GCAATG	1 cut(s)	808
BseMII	CTCAG	4 cut(s)	364, 482, 1272, 1404
BseNI	ACTGG	3 cut(s)	911, 1586, 1597
BseRI	GAGGAG	2 cut(s)	203, 1673
BseSI	GKGCMC	1 cut(s)	47
BseXI	GCAGC	9 cut(s)	69, 242, 245, 271, 605, 700, 715, 1057, 1771
BsgI	GTGCAG	2 cut(s)	1064, 1185
Bsh1236I	CGCG	2 cut(s)	307, 1056
Bsh1285I	CGRYCG	3 cut(s)	305, 429, 1054
BshFI	GGCC	3 cut(s)	775, 867, 1074
BshNI	GGYRCC	1 cut(s)	71
BshVI	ATCGAT	1 cut(s)	429
BsiEI	CGRYCG	3 cut(s)	305, 429, 1054
BsiHKAI	GWGCWC	4 cut(s)	47, 882, 923, 1117
BsiHKCI	CYCGRG	1 cut(s)	236
BslFI	GGGAC	3 cut(s)	173, 451, 1304
BslI	CCNNNNNNNGG	4 cut(s)	237, 1082, 1117, 1618
BsmAI	GTCTC	3 cut(s)	684, 1277, 1673
BsmFI	GGGAC	3 cut(s)	173, 451, 1304
BsnI	GGCC	3 cut(s)	775, 867, 1074
BsoBI	CYCGRG	1 cut(s)	236
Bsp1286I	GDGCHC	7 cut(s)	47, 237, 243, 351, 882, 923, 1117
Bsp143I	GATC	8 cut(s)	426, 430, 658, 1051, 1167, 1361, 1482, 1548
BspACI	CCGC	9 cut(s)	75, 148, 307, 567, 579, 725, 1335, 1527, 1795
BspANI	GGCC	3 cut(s)	775, 867, 1074
BspCNI	CTCAG	4 cut(s)	363, 483, 1273, 1405
BspDI	ATCGAT	1 cut(s)	429
BspFNI	CGCG	2 cut(s)	307, 1056
BspHI	TCATGA	2 cut(s)	550, 1150
BspLI	GGNNCC	6 cut(s)	19, 73, 418, 660, 774, 1561
BspMAI	CTGCAG	2 cut(s)	733, 955
BspPI	GGATC	6 cut(s)	653, 666, 1175, 1369, 1477, 1543
BspT107I	GGYRCC	1 cut(s)	71
BsrDI	GCAATG	1 cut(s)	808
BsrFI	RCCGGY	3 cut(s)	37, 757, 1324
BsrI	ACTGG	3 cut(s)	911, 1586, 1597
BssAI	RCCGGY	3 cut(s)	37, 757, 1324
BssECI	CCNNGG	5 cut(s)	190, 236, 717, 1641, 1689
BssMI	GATC	8 cut(s)	426, 430, 658, 1051, 1167, 1361, 1482, 1548
BssNI	GRCGYC	4 cut(s)	51, 72, 123, 168
BssT1I	CCWWGG	1 cut(s)	1641
Bst2UI	CCWGG	1 cut(s)	1454
Bst4CI	ACNGT	6 cut(s)	109, 187, 340, 383, 508, 1462
Bst6I	CTCTTC	1 cut(s)	1650
BstACI	GRCGYC	4 cut(s)	51, 72, 123, 168
BstAPI	GCANNNNNTGC	1 cut(s)	618
BstC8I	GCNNGC	7 cut(s)	35, 39, 43, 148, 596, 799, 1326
BstDEI	CTNAG	6 cut(s)	350, 491, 706, 1281, 1413, 1809
BstDSI	CCRYGG	1 cut(s)	717
BstEII	GGTNACC	1 cut(s)	468
BstF5I	GGATG	3 cut(s)	640, 1242, 1608
BstFNI	CGCG	2 cut(s)	307, 1056
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	2 cut(s)	74, 935
BstKTI	GATC	8 cut(s)	429, 433, 661, 1054, 1170, 1364, 1485, 1551
BstMAI	GTCTC	3 cut(s)	684, 1277, 1673
BstMBI	GATC	8 cut(s)	426, 430, 658, 1051, 1167, 1361, 1482, 1548
BstMCI	CGRYCG	3 cut(s)	305, 429, 1054
BstMWI	GCNNNNNNNGC	8 cut(s)	80, 281, 618, 722, 803, 932, 1277, 1691
BstNI	CCWGG	1 cut(s)	1454
BstNSI	RCATGY	1 cut(s)	797
BstPI	GGTNACC	1 cut(s)	468
BstSCI	CCNGG	7 cut(s)	190, 235, 236, 275, 654, 1452, 1688
BstSFI	CTRYAG	4 cut(s)	729, 951, 991, 1159
BstSLI	GKGCMC	1 cut(s)	47
BstUI	CGCG	2 cut(s)	307, 1056
BstV1I	GCAGC	9 cut(s)	69, 242, 245, 271, 605, 700, 715, 1057, 1771
BstX2I	RGATCY	3 cut(s)	658, 1167, 1548
BstXI	CCANNNNNNTGG	2 cut(s)	464, 896
BstYI	RGATCY	3 cut(s)	658, 1167, 1548
Bsu15I	ATCGAT	1 cut(s)	429
BsuI	GTATCC	2 cut(s)	998, 1523
BsuRI	GGCC	3 cut(s)	775, 867, 1074
BsuTUI	ATCGAT	1 cut(s)	429
BtgI	CCRYGG	1 cut(s)	717
BtgZI	GCGATG	3 cut(s)	127, 211, 1455
BtrI	CACGTC	1 cut(s)	178
BtsCI	GGATG	3 cut(s)	640, 1242, 1608
BtsI	GCAGTG	3 cut(s)	93, 756, 921
BtsIMutI	CAGTG	9 cut(s)	93, 171, 183, 336, 358, 388, 756, 921, 1590
Cac8I	GCNNGC	7 cut(s)	35, 39, 43, 148, 596, 799, 1326
CaiI	CAGNNNCTG	1 cut(s)	1315
CciI	TCATGA	2 cut(s)	550, 1150
CfoI	GCGC	2 cut(s)	74, 935
Cfr10I	RCCGGY	3 cut(s)	37, 757, 1324
Cfr13I	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
Cfr9I	CCCGGG	1 cut(s)	236
ClaI	ATCGAT	1 cut(s)	429
CseI	GACGC	3 cut(s)	131, 157, 448
Csp6I	GTAC	1 cut(s)	280
CspCI	CAANNNNNGTGG	2 cut(s)	1524, 1559
CviAII	CATG	7 cut(s)	551, 794, 850, 1151, 1296, 1480, 1553
CviQI	GTAC	1 cut(s)	280
DdeI	CTNAG	6 cut(s)	350, 491, 706, 1281, 1413, 1809
DinI	GGCGCC	1 cut(s)	73
DpnI	GATC	8 cut(s)	428, 432, 660, 1053, 1169, 1363, 1484, 1550
DpnII	GATC	8 cut(s)	426, 430, 658, 1051, 1167, 1361, 1482, 1548
Eam1104I	CTCTTC	1 cut(s)	1650
EarI	CTCTTC	1 cut(s)	1650
EciI	GGCGGA	1 cut(s)	1542
Ecl136II	GAGCTC	1 cut(s)	1115
Eco130I	CCWWGG	1 cut(s)	1641
Eco24I	GRGCYC	4 cut(s)	237, 243, 351, 1117
Eco47I	GGWCC	4 cut(s)	416, 974, 1559, 1645
Eco53kI	GAGCTC	1 cut(s)	1115
Eco88I	CYCGRG	1 cut(s)	236
Eco91I	GGTNACC	1 cut(s)	468
EcoICRI	GAGCTC	1 cut(s)	1115
EcoO109I	RGGNCCY	3 cut(s)	416, 773, 865
EcoO65I	GGTNACC	1 cut(s)	468
EcoRI	GAATTC	1 cut(s)	633
EcoRII	CCWGG	1 cut(s)	1452
EcoT14I	CCWWGG	1 cut(s)	1641
EcoT22I	ATGCAT	1 cut(s)	961
EcoT38I	GRGCYC	4 cut(s)	237, 243, 351, 1117
EgeI	GGCGCC	1 cut(s)	73
EheI	GGCGCC	1 cut(s)	73
ErhI	CCWWGG	1 cut(s)	1641
FaeI	CATG	7 cut(s)	554, 797, 853, 1154, 1299, 1483, 1556
FalI	AAGNNNNNCTT	4 cut(s)	1691, 1723, 1743, 1775
FaqI	GGGAC	3 cut(s)	173, 451, 1304
FatI	CATG	7 cut(s)	550, 793, 849, 1150, 1295, 1479, 1552
FauI	CCCGC	3 cut(s)	155, 300, 1328
FokI	GGATG	3 cut(s)	627, 1229, 1595
FriOI	GRGCYC	4 cut(s)	237, 243, 351, 1117
FspBI	CTAG	4 cut(s)	818, 828, 1598, 1773
GlaI	GCGC	2 cut(s)	73, 934
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	3 cut(s)	775, 867, 1074
HgaI	GACGC	3 cut(s)	131, 157, 448
HhaI	GCGC	2 cut(s)	74, 935
Hin1I	GRCGYC	4 cut(s)	51, 72, 123, 168
Hin1II	CATG	7 cut(s)	554, 797, 853, 1154, 1299, 1483, 1556
Hin6I	GCGC	2 cut(s)	72, 933
HinP1I	GCGC	2 cut(s)	72, 933
HinfI	GANTC	6 cut(s)	153, 312, 388, 448, 947, 1292
HphI	GGTGA	5 cut(s)	206, 235, 446, 1468, 1735
Hpy166II	GTNNAC	3 cut(s)	45, 183, 1476
Hpy188I	TCNGA	4 cut(s)	121, 1095, 1112, 1487
Hpy8I	GTNNAC	3 cut(s)	45, 183, 1476
Hpy99I	CGWCG	3 cut(s)	29, 53, 56
HpyAV	CCTTC	4 cut(s)	1024, 1086, 1093, 1693
HpyCH4III	ACNGT	6 cut(s)	109, 187, 340, 383, 508, 1462
HpyCH4IV	ACGT	3 cut(s)	27, 51, 177
HpyF10VI	GCNNNNNNNGC	8 cut(s)	80, 281, 618, 722, 803, 932, 1277, 1691
HpyF3I	CTNAG	6 cut(s)	350, 491, 706, 1281, 1413, 1809
HpySE526I	ACGT	3 cut(s)	27, 51, 177
Hsp92I	GRCGYC	4 cut(s)	51, 72, 123, 168
Hsp92II	CATG	7 cut(s)	554, 797, 853, 1154, 1299, 1483, 1556
HspAI	GCGC	2 cut(s)	72, 933
KasI	GGCGCC	1 cut(s)	71
KroI	GCCGGC	2 cut(s)	37, 1324
KroNI	GCCGGC	2 cut(s)	39, 1326
Kzo9I	GATC	8 cut(s)	426, 430, 658, 1051, 1167, 1361, 1482, 1548
LmnI	GCTCC	4 cut(s)	374, 1001, 1382, 1660
Lsp1109I	GCAGC	9 cut(s)	69, 242, 245, 271, 605, 700, 715, 1057, 1771
LweI	GCATC	6 cut(s)	485, 535, 814, 946, 1341, 1594
MaeI	CTAG	4 cut(s)	818, 828, 1598, 1773
MaeII	ACGT	3 cut(s)	27, 51, 177
MaeIII	GTNAC	7 cut(s)	169, 194, 328, 468, 508, 1057, 1456
MalI	GATC	8 cut(s)	428, 432, 660, 1053, 1169, 1363, 1484, 1550
MboI	GATC	8 cut(s)	426, 430, 658, 1051, 1167, 1361, 1482, 1548
MboII	GAAGA	6 cut(s)	443, 554, 1501, 1510, 1667, 1708
MfeI	CAATTG	3 cut(s)	1035, 1365, 1536
MflI	RGATCY	3 cut(s)	658, 1167, 1548
MhlI	GDGCHC	7 cut(s)	47, 237, 243, 351, 882, 923, 1117
MluCI	AATT	8 cut(s)	112, 570, 633, 1035, 1249, 1319, 1365, 1536
Mly113I	GGCGCC	1 cut(s)	72
MlyI	GAGTC	4 cut(s)	147, 321, 956, 1286
MmeI	TCCRAC	4 cut(s)	391, 982, 1261, 1360
Mph1103I	ATGCAT	1 cut(s)	961
MroNI	GCCGGC	2 cut(s)	37, 1324
MroXI	GAANNNNTTC	1 cut(s)	1103
MseI	TTAA	4 cut(s)	201, 1224, 1302, 1575
MslI	CAYNNNNRTG	2 cut(s)	176, 1721
MspR9I	CCNGG	7 cut(s)	192, 237, 238, 277, 656, 1454, 1690
MunI	CAATTG	3 cut(s)	1035, 1365, 1536
MvaI	CCWGG	1 cut(s)	1454
MvnI	CGCG	2 cut(s)	307, 1056
MwoI	GCNNNNNNNGC	8 cut(s)	80, 281, 618, 722, 803, 932, 1277, 1691
NaeI	GCCGGC	2 cut(s)	39, 1326
NarI	GGCGCC	1 cut(s)	72
NciI	CCSGG	6 cut(s)	192, 237, 238, 277, 656, 1690
NdeII	GATC	8 cut(s)	426, 430, 658, 1051, 1167, 1361, 1482, 1548
NgoMIV	GCCGGC	2 cut(s)	37, 1324
NlaIII	CATG	7 cut(s)	554, 797, 853, 1154, 1299, 1483, 1556
NlaIV	GGNNCC	6 cut(s)	19, 73, 418, 660, 774, 1561
NmeAIII	GCCGAG	2 cut(s)	256, 961
NmuCI	GTSAC	5 cut(s)	169, 194, 508, 1057, 1456
NsiI	ATGCAT	1 cut(s)	961
NspI	RCATGY	1 cut(s)	797
OliI	CACNNNNGTG	1 cut(s)	176
PagI	TCATGA	2 cut(s)	550, 1150
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PdiI	GCCGGC	2 cut(s)	39, 1326
PdmI	GAANNNNTTC	1 cut(s)	1103
PfeI	GAWTC	2 cut(s)	388, 448
Ple19I	CGATCG	2 cut(s)	429, 1054
PleI	GAGTC	4 cut(s)	147, 320, 955, 1286
PluTI	GGCGCC	1 cut(s)	75
PpsI	GAGTC	4 cut(s)	147, 320, 955, 1286
PpuMI	RGGWCCY	1 cut(s)	416
Psp124BI	GAGCTC	1 cut(s)	1117
Psp5II	RGGWCCY	1 cut(s)	416
Psp6I	CCWGG	1 cut(s)	1452
PspEI	GGTNACC	1 cut(s)	468
PspGI	CCWGG	1 cut(s)	1452
PspN4I	GGNNCC	6 cut(s)	19, 73, 418, 660, 774, 1561
PspPI	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
PspPPI	RGGWCCY	1 cut(s)	416
PsrI	GAACNNNNNNTAC	2 cut(s)	1571, 1603
PstI	CTGCAG	2 cut(s)	733, 955
PstNI	CAGNNNCTG	1 cut(s)	1315
PsuI	RGATCY	3 cut(s)	658, 1167, 1548
PvuI	CGATCG	2 cut(s)	429, 1054
RsaI	GTAC	1 cut(s)	281
RsaNI	GTAC	1 cut(s)	280
RseI	CAYNNNNRTG	2 cut(s)	176, 1721
SacI	GAGCTC	1 cut(s)	1117
SaqAI	TTAA	4 cut(s)	201, 1224, 1302, 1575
Sau3AI	GATC	8 cut(s)	426, 430, 658, 1051, 1167, 1361, 1482, 1548
Sau96I	GGNCC	7 cut(s)	416, 773, 865, 974, 1073, 1559, 1645
SchI	GAGTC	4 cut(s)	147, 321, 956, 1286
ScrFI	CCNGG	7 cut(s)	192, 237, 238, 277, 656, 1454, 1690
SduI	GDGCHC	7 cut(s)	47, 237, 243, 351, 882, 923, 1117
SfaNI	GCATC	6 cut(s)	485, 535, 814, 946, 1341, 1594
SfcI	CTRYAG	4 cut(s)	729, 951, 991, 1159
SfoI	GGCGCC	1 cut(s)	73
SinI	GGWCC	4 cut(s)	416, 974, 1559, 1645
SmaI	CCCGGG	1 cut(s)	238
SmiMI	CAYNNNNRTG	2 cut(s)	176, 1721
SmlI	CTYRAG	4 cut(s)	1019, 1130, 1401, 1760
SmoI	CTYRAG	4 cut(s)	1019, 1130, 1401, 1760
SpeI	ACTAGT	1 cut(s)	1597
SrfI	GCCCGGGC	1 cut(s)	238
Sse9I	AATT	8 cut(s)	112, 570, 633, 1035, 1249, 1319, 1365, 1536
SsiI	CCGC	9 cut(s)	75, 148, 307, 567, 579, 725, 1335, 1527, 1795
SspDI	GGCGCC	1 cut(s)	71
SspMI	CTAG	4 cut(s)	818, 828, 1598, 1773
SstI	GAGCTC	1 cut(s)	1117
StyD4I	CCNGG	7 cut(s)	190, 235, 236, 275, 654, 1452, 1688
StyI	CCWWGG	1 cut(s)	1641
TaaI	ACNGT	6 cut(s)	109, 187, 340, 383, 508, 1462
TaiI	ACGT	3 cut(s)	30, 54, 180
TaqI	TCGA	5 cut(s)	24, 216, 425, 429, 784
TaqII	GACCGA	2 cut(s)	291, 991
TasI	AATT	8 cut(s)	112, 570, 633, 1035, 1249, 1319, 1365, 1536
TauI	GCSGC	3 cut(s)	77, 728, 1797
TfiI	GAWTC	2 cut(s)	388, 448
Tru1I	TTAA	4 cut(s)	201, 1224, 1302, 1575
Tru9I	TTAA	4 cut(s)	201, 1224, 1302, 1575
TscAI	CASTG	9 cut(s)	100, 178, 190, 343, 358, 388, 756, 928, 1597
TseFI	GTSAC	5 cut(s)	169, 194, 508, 1057, 1456
TseI	GCWGC	9 cut(s)	57, 255, 258, 284, 618, 713, 728, 1045, 1784
Tsp45I	GTSAC	5 cut(s)	169, 194, 508, 1057, 1456
TspDTI	ATGAA	6 cut(s)	567, 883, 1068, 1112, 1423, 1705
TspGWI	ACGGA	4 cut(s)	657, 988, 1572, 1637
TspMI	CCCGGG	1 cut(s)	236
TspRI	CASTG	9 cut(s)	100, 178, 190, 343, 358, 388, 756, 928, 1597
VneI	GTGCAC	1 cut(s)	43
VpaK11BI	GGWCC	4 cut(s)	416, 974, 1559, 1645
XapI	RAATTY	2 cut(s)	633, 1249
XceI	RCATGY	1 cut(s)	797
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1355
XmaI	CCCGGG	1 cut(s)	236
XmnI	GAANNNNTTC	1 cut(s)	1103
XspI	CTAG	4 cut(s)	818, 828, 1598, 1773
ZraI	GACGTC	1 cut(s)	52
Zsp2I	ATGCAT	1 cut(s)	961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.