Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0003299

Physical Location & Seq

Genomic Coordinates Forward (+)

17249675 .. 17252276

Gene Structure 2602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGGACACCAAAATCGGTTCCCTCGACGTTTGCAAGCCGGCTTGCACCGACGTCGGCAGCCTCCCGAACGGCGCCACTTCGGCAATCCAAAGCACTGCCCCCTCCACCGTCAACTCCTCAGATGCCACTCTGGGTCGCCACATCGCCCGCCGACTCGTCCAAATCGGCGTTACTGACGTGTTCACTGTCCCTGGTGACTTTAACTTGACCCTCCTCGACCACCTCATCGCGGAGCCCGGGCTCACCAACATCGGCTGCTGCAACGAACTCAACGCCGGGTACGCTACTGACGGCTACGCTAGGTCGCGGGGAGTCGGGGCGTGTGTTGTTACTTTCACTGTGGGTGGGCTCAGTGTTCTCAATGCTATCGCCGGAGCTTACAGTGAGAATCTGCCATTGATTTGTATAGTTGGAGGACCCAACTCGAACGATTACGGGACGCACAGGATTCTTCACCACACTATTGGGTTACCGGATTTTAACCAAGAGCTGAGATGCTTCCAGACCGTCACTTGCTATCAGGCTGTGGTAAATAATCTGGAAGATGCTCATGAAATGATTGATACCGCAATTTCAACCGCCTTGAAAGAAAGCAAGCCTGTTTATATCAGCATAAGCTGCAACTTGGCTGGAATTCCTCATCCTACTTTTAGCCGGGATCCTGTTCCGTTCTCATTGTCTCCAAGATTGAGCAATCATTTGGGCTTAAAGGCTGCCGTGGAGGCGGCTGCAGAGTTCTTGAACAAGGCAGTGAAGCCGGTTATGGTAGGGGGGCCTAAACTTCGAGTTGCACACGCTGGCGATGCCTTTGTTGAACTAGCTGACGCTAGTGGTTATGCTCTGGCTGTCATGCCATCTGCAAAGGGGCTTGTGCCAGAGCACCACCCCCATTTCATTGGAACATACTGGGGTGCTGTGAGCACTACCTTTTGCGCCGAGATTGTGGAGTCTGCAGATGCATACTTGTTTGATGGACCGATTTTCAATGACTACAGCTCTGTTGGATACTCTCTGCTTCTGAAGAAAGAGAAGGCAATTGTGGTGCAGCCTGATCGCGTGACCATAGAAAATGGCCCTTCATTTGGTTGTGTTCTCATGAAGGATTTCCTCCGAGCTCTGGCAAAGAGGCTCAAGCGCAACAAAACTGCTCATGAGAACTACAGCAGATCGATCTTTGTTCCCAATGGACGTCCTCTAAAGTCTGCACCGAAAGAACCTTTGAGGGTTAATGTTCTGTTCCACCACATCCAGAATACGCTGTCAAGTGAAACTGCTGTGATTGCTGATACCGGGGACTCATGGTTTAACTGCCAGAAACTGAAATTGCCGGCTGGTTGCGGGTATGAGTTCCAAATGCAGTATGGATCAATCTATGAACTAATCCATGGTTGGTCAGTTGGAGCTACTCTTGGGTATGCTCAAGCTGTTCCTGAGAAGCGTGTGATTGCTTTCATCGGCGATGGGAGTTTCCAGGTGACTGTTCAAGATGTGTCCACCATGATCCGAAATGGGCAGAAGAACATCATCTTCCTGATAAACAACGGCGGATACACAATTGGGGTGGTGATCCATGACGGACCCTACAATGTGATCAAGAACTGGAACTACACTGGACTAGTTGATGCCATCCACAACGGGGAGGGCAAGTGCTGGACAACCAAGGTCCGTTGCGAAGAGGAGCTGATTGAAGCGATTGAGACTGCAACCGGGGCGAAGAAGGATAGCTTGTGCTTCATTGAGGTGATAGCCCACAAGGATGATACCAGCAAAGAGTTGCTTCAGTGGGGGTCTAGGGTTTCTGCTGCCAACAGCCGCCCACCAACCCTCAGTAAAGTCTCCCATCTCGTTTCCTTTCATCATCGTAGCGTCTTTCGATCTGGAAGAAGAAAAAAAATTAGTATTTGTTCCTTCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0008150 GO:0009628 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0034059 GO:0036293 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0070482

KEGG Pathways

Metabolic & Signaling

ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

639

Amino Acids

69.02

Weight (kDa)

7.29

Isoelectric Point (pI)

34.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	43 - 148	1.3e-28	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	240 - 358	2e-21	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	461 - 581	1.7e-14	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003299)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33070 AT5G01320 AT5G01330
fragaria_vesca	FvH4_6g11100
malus_domestica	MD04G1159900.v1.1 MD04G1160100.v1.1 MD12G1172400.v1.1 MD12G1172500.v1.1 MD12G1173200.v1.1
prunus_persica	Prupe.6G280800_v2.0.a1 Prupe.6G280800_v2.0.a1
pyrus_communis	pycom04g14330 pycom04g14350 pycom12g16360 pycom12g16370 pycom17g25190
rosa_chinensis	RchiOBHm_Chr3g0462531 RchiOBHm_Chr7g0223061
rosa_laevigata	RLG00000024851 RLG00000024852
rosa_multiflora	Rmu_sc0003624.1_g000016 Rmu_sc0011346.1_g000001 Rmu_sc0025654.1_g000001
rosa_roxburghii	Rroxscaffold_5G00335980 Rroxscaffold_6G00417420 Rroxscaffold_6G00417440 Rroxscaffold_6G00417450
rosa_rugosa	Rorug03G0057300 Rorug03G0057400 Rorug03G0141600
rosa_samantha	Rh3AG115300 Rh3BG118700 Rh3CG122100 Rh3DG120800 Rh4AG098400
rosa_wichuraiana	Rw3G009760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	54, 1192
AccB1I	GGYRCC	1 cut(s)	71
AccII	CGCG	3 cut(s)	230, 307, 1056
AciI	CCGC	9 cut(s)	148, 230, 307, 567, 579, 725, 1338, 1545, 1813
AclWI	GGATC	5 cut(s)	653, 666, 1372, 1495, 1561
AcsI	RAATTY	1 cut(s)	633
AcuI	CTGAAG	2 cut(s)	1040, 1763
AcyI	GRCGYC	3 cut(s)	51, 72, 1189
AfaI	GTAC	1 cut(s)	281
AfiI	CCNNNNNNNGG	4 cut(s)	229, 1082, 1117, 1636
AflIII	ACRYGT	1 cut(s)	177
AgsI	TTSAA	7 cut(s)	576, 586, 742, 815, 985, 1484, 1688
AhlI	ACTAGT	1 cut(s)	1615
AjiI	CACGTC	1 cut(s)	178
AjnI	CCWGG	2 cut(s)	190, 1470
Alw21I	GWGCWC	3 cut(s)	882, 923, 1117
Alw26I	GTCTC	3 cut(s)	684, 1691, 1840
AlwI	GGATC	5 cut(s)	653, 666, 1372, 1495, 1561
AlwNI	CAGNNNCTG	1 cut(s)	1318
Ama87I	CYCGRG	1 cut(s)	236
AoxI	GGCC	2 cut(s)	773, 1072
ApeKI	GCWGC	8 cut(s)	57, 255, 258, 618, 713, 728, 1045, 1802
ApoI	RAATTY	1 cut(s)	633
Asp700I	GAANNNNTTC	1 cut(s)	1103
AspLEI	GCGC	3 cut(s)	74, 935, 1137
AspS9I	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
AsuC2I	CCSGG	6 cut(s)	237, 238, 277, 656, 1291, 1708
AsuHPI	GGTGA	6 cut(s)	206, 235, 446, 1486, 1576, 1753
AvaI	CYCGRG	1 cut(s)	236
AvaII	GGWCC	4 cut(s)	416, 974, 1577, 1663
BamHI	GGATCC	1 cut(s)	658
BanI	GGYRCC	1 cut(s)	71
BanII	GRGCYC	4 cut(s)	237, 243, 351, 1117
Bbv12I	GWGCWC	3 cut(s)	882, 923, 1117
BbvI	GCAGC	8 cut(s)	69, 242, 245, 605, 700, 715, 1057, 1789
BccI	CCATC	5 cut(s)	862, 965, 1454, 1634, 1848
BceAI	ACGGC	4 cut(s)	85, 307, 701, 1558
BcgI	CGANNNNNNTGC	4 cut(s)	34, 68, 1034, 1068
BciT130I	CCWGG	2 cut(s)	192, 1472
BciVI	GTATCC	2 cut(s)	998, 1541
BclI	TGATCA	1 cut(s)	1590
BcnI	CCSGG	6 cut(s)	237, 238, 277, 656, 1291, 1708
BcoDI	GTCTC	3 cut(s)	684, 1691, 1840
BcuI	ACTAGT	1 cut(s)	1615
BfaI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
BfmI	CTRYAG	4 cut(s)	729, 951, 991, 1159
BfoI	RGCGCY	1 cut(s)	75
BfuI	GTATCC	2 cut(s)	998, 1541
BglI	GCCNNNNNGGC	2 cut(s)	80, 722
Bme1390I	CCNGG	8 cut(s)	192, 237, 238, 277, 656, 1291, 1472, 1708
Bme18I	GGWCC	4 cut(s)	416, 974, 1577, 1663
BmeT110I	CYCGRG	1 cut(s)	236
BmgBI	CACGTC	1 cut(s)	178
BmgT120I	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
BmiI	GGNNCC	7 cut(s)	19, 73, 234, 418, 660, 774, 1579
BmrFI	CCNGG	8 cut(s)	192, 237, 238, 277, 656, 1291, 1472, 1708
BmrI	ACTGGG	1 cut(s)	916
BmsI	GCATC	6 cut(s)	112, 485, 535, 793, 946, 1612
BmuI	ACTGGG	1 cut(s)	916
BpuEI	CTTGAG	2 cut(s)	1115, 1404
BpuMI	CCSGG	6 cut(s)	237, 238, 277, 656, 1291, 1708
Bsa29I	ATCGAT	1 cut(s)	1169
BsaHI	GRCGYC	3 cut(s)	51, 72, 1189
BsaJI	CCNNGG	7 cut(s)	190, 236, 717, 1290, 1384, 1659, 1707
BsaWI	WCCGGW	1 cut(s)	472
BsaXI	ACNNNNNCTCC	2 cut(s)	1631, 1661
Bsc4I	CCNNNNNNNGG	4 cut(s)	229, 1082, 1117, 1636
Bse118I	RCCGGY	3 cut(s)	37, 757, 1327
Bse1I	ACTGG	3 cut(s)	911, 1604, 1615
BseBI	CCWGG	2 cut(s)	192, 1472
BseCI	ATCGAT	1 cut(s)	1169
BseDI	CCNNGG	7 cut(s)	190, 236, 717, 1290, 1384, 1659, 1707
BseGI	GGATG	4 cut(s)	640, 1245, 1626, 1762
BseLI	CCNNNNNNNGG	4 cut(s)	229, 1082, 1117, 1636
BseMII	CTCAG	5 cut(s)	132, 364, 482, 1422, 1840
BseNI	ACTGG	3 cut(s)	911, 1604, 1615
BseRI	GAGGAG	3 cut(s)	106, 203, 1691
BseXI	GCAGC	8 cut(s)	69, 242, 245, 605, 700, 715, 1057, 1789
BsgI	GTGCAG	2 cut(s)	1064, 1188
Bsh1236I	CGCG	3 cut(s)	230, 307, 1056
BshFI	GGCC	2 cut(s)	775, 1074
BshNI	GGYRCC	1 cut(s)	71
BshVI	ATCGAT	1 cut(s)	1169
BsiHKAI	GWGCWC	3 cut(s)	882, 923, 1117
BsiHKCI	CYCGRG	1 cut(s)	236
BslFI	GGGAC	3 cut(s)	173, 451, 1307
BslI	CCNNNNNNNGG	4 cut(s)	229, 1082, 1117, 1636
BsmAI	GTCTC	3 cut(s)	684, 1691, 1840
BsmFI	GGGAC	3 cut(s)	173, 451, 1307
BsnI	GGCC	2 cut(s)	775, 1074
BsoBI	CYCGRG	1 cut(s)	236
Bsp1286I	GDGCHC	6 cut(s)	237, 243, 351, 882, 923, 1117
Bsp143I	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
Bsp19I	CCATGG	1 cut(s)	1384
BspACI	CCGC	9 cut(s)	148, 230, 307, 567, 579, 725, 1338, 1545, 1813
BspANI	GGCC	2 cut(s)	775, 1074
BspCNI	CTCAG	5 cut(s)	131, 363, 483, 1423, 1839
BspDI	ATCGAT	1 cut(s)	1169
BspFNI	CGCG	3 cut(s)	230, 307, 1056
BspHI	TCATGA	3 cut(s)	550, 1095, 1150
BspLI	GGNNCC	7 cut(s)	19, 73, 234, 418, 660, 774, 1579
BspMAI	CTGCAG	2 cut(s)	733, 955
BspPI	GGATC	5 cut(s)	653, 666, 1372, 1495, 1561
BspT107I	GGYRCC	1 cut(s)	71
BsrFI	RCCGGY	3 cut(s)	37, 757, 1327
BsrI	ACTGG	3 cut(s)	911, 1604, 1615
BssAI	RCCGGY	3 cut(s)	37, 757, 1327
BssECI	CCNNGG	7 cut(s)	190, 236, 717, 1290, 1384, 1659, 1707
BssMI	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
BssNI	GRCGYC	3 cut(s)	51, 72, 1189
BssT1I	CCWWGG	2 cut(s)	1384, 1659
Bst2UI	CCWGG	2 cut(s)	192, 1472
Bst4CI	ACNGT	6 cut(s)	109, 187, 340, 383, 508, 1480
Bst6I	CTCTTC	1 cut(s)	1668
BstACI	GRCGYC	3 cut(s)	51, 72, 1189
BstAPI	GCANNNNNTGC	1 cut(s)	618
BstC8I	GCNNGC	7 cut(s)	35, 39, 43, 148, 596, 799, 1329
BstDEI	CTNAG	5 cut(s)	118, 350, 491, 1431, 1826
BstDSI	CCRYGG	2 cut(s)	717, 1384
BstEII	GGTNACC	1 cut(s)	468
BstF5I	GGATG	4 cut(s)	640, 1245, 1626, 1762
BstFNI	CGCG	3 cut(s)	230, 307, 1056
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	3 cut(s)	74, 935, 1137
BstKTI	GATC	9 cut(s)	661, 1054, 1169, 1173, 1367, 1503, 1569, 1593, 1877
BstMAI	GTCTC	3 cut(s)	684, 1691, 1840
BstMBI	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
BstMWI	GCNNNNNNNGC	7 cut(s)	80, 281, 618, 722, 803, 1280, 1709
BstNI	CCWGG	2 cut(s)	192, 1472
BstPI	GGTNACC	1 cut(s)	468
BstSCI	CCNGG	8 cut(s)	190, 235, 236, 275, 654, 1289, 1470, 1706
BstSFI	CTRYAG	4 cut(s)	729, 951, 991, 1159
BstUI	CGCG	3 cut(s)	230, 307, 1056
BstV1I	GCAGC	8 cut(s)	69, 242, 245, 605, 700, 715, 1057, 1789
BstX2I	RGATCY	1 cut(s)	658
BstXI	CCANNNNNNTGG	2 cut(s)	464, 896
BstYI	RGATCY	1 cut(s)	658
Bsu15I	ATCGAT	1 cut(s)	1169
BsuI	GTATCC	2 cut(s)	998, 1541
BsuRI	GGCC	2 cut(s)	775, 1074
BsuTUI	ATCGAT	1 cut(s)	1169
BtgI	CCRYGG	2 cut(s)	717, 1384
BtgZI	GCGATG	4 cut(s)	127, 211, 816, 1473
BtrI	CACGTC	1 cut(s)	178
BtsCI	GGATG	4 cut(s)	640, 1245, 1626, 1762
BtsI	GCAGTG	2 cut(s)	93, 756
BtsIMutI	CAGTG	8 cut(s)	93, 183, 336, 358, 388, 756, 1608, 1787
Cac8I	GCNNGC	7 cut(s)	35, 39, 43, 148, 596, 799, 1329
CaiI	CAGNNNCTG	1 cut(s)	1318
CciI	TCATGA	3 cut(s)	550, 1095, 1150
CfoI	GCGC	3 cut(s)	74, 935, 1137
Cfr10I	RCCGGY	3 cut(s)	37, 757, 1327
Cfr13I	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
Cfr9I	CCCGGG	1 cut(s)	236
ClaI	ATCGAT	1 cut(s)	1169
CseI	GACGC	3 cut(s)	448, 833, 1855
Csp6I	GTAC	1 cut(s)	280
CspCI	CAANNNNNGTGG	2 cut(s)	1542, 1577
CviAII	CATG	8 cut(s)	551, 850, 1096, 1151, 1299, 1385, 1498, 1571
CviQI	GTAC	1 cut(s)	280
DdeI	CTNAG	5 cut(s)	118, 350, 491, 1431, 1826
DinI	GGCGCC	1 cut(s)	73
DpnI	GATC	9 cut(s)	660, 1053, 1168, 1172, 1366, 1502, 1568, 1592, 1876
DpnII	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
Eam1104I	CTCTTC	1 cut(s)	1668
EarI	CTCTTC	1 cut(s)	1668
EciI	GGCGGA	1 cut(s)	1560
Ecl136II	GAGCTC	1 cut(s)	1115
Eco130I	CCWWGG	2 cut(s)	1384, 1659
Eco24I	GRGCYC	4 cut(s)	237, 243, 351, 1117
Eco47I	GGWCC	4 cut(s)	416, 974, 1577, 1663
Eco53kI	GAGCTC	1 cut(s)	1115
Eco57I	CTGAAG	2 cut(s)	1040, 1763
Eco88I	CYCGRG	1 cut(s)	236
Eco91I	GGTNACC	1 cut(s)	468
EcoICRI	GAGCTC	1 cut(s)	1115
EcoO109I	RGGNCCY	2 cut(s)	416, 773
EcoO65I	GGTNACC	1 cut(s)	468
EcoRI	GAATTC	1 cut(s)	633
EcoRII	CCWGG	2 cut(s)	190, 1470
EcoT14I	CCWWGG	2 cut(s)	1384, 1659
EcoT22I	ATGCAT	1 cut(s)	961
EcoT38I	GRGCYC	4 cut(s)	237, 243, 351, 1117
EgeI	GGCGCC	1 cut(s)	73
EheI	GGCGCC	1 cut(s)	73
ErhI	CCWWGG	2 cut(s)	1384, 1659
FaeI	CATG	8 cut(s)	554, 853, 1099, 1154, 1302, 1388, 1501, 1574
FalI	AAGNNNNNCTT	4 cut(s)	1709, 1741, 1761, 1793
FaqI	GGGAC	3 cut(s)	173, 451, 1307
FatI	CATG	8 cut(s)	550, 849, 1095, 1150, 1298, 1384, 1497, 1570
FauI	CCCGC	3 cut(s)	155, 300, 1331
FbaI	TGATCA	1 cut(s)	1590
FokI	GGATG	4 cut(s)	627, 1232, 1613, 1769
FriOI	GRGCYC	4 cut(s)	237, 243, 351, 1117
FspBI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
GlaI	GCGC	3 cut(s)	73, 934, 1136
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	2 cut(s)	775, 1074
HgaI	GACGC	3 cut(s)	448, 833, 1855
HhaI	GCGC	3 cut(s)	74, 935, 1137
Hin1I	GRCGYC	3 cut(s)	51, 72, 1189
Hin1II	CATG	8 cut(s)	554, 853, 1099, 1154, 1302, 1388, 1501, 1574
Hin6I	GCGC	3 cut(s)	72, 933, 1135
HinP1I	GCGC	3 cut(s)	72, 933, 1135
HincII	GTYRAC	1 cut(s)	112
HindII	GTYRAC	1 cut(s)	112
HinfI	GANTC	6 cut(s)	153, 312, 388, 448, 947, 1295
HphI	GGTGA	6 cut(s)	206, 235, 446, 1486, 1576, 1753
Hpy166II	GTNNAC	3 cut(s)	112, 183, 1494
Hpy188I	TCNGA	4 cut(s)	121, 1020, 1112, 1505
Hpy8I	GTNNAC	3 cut(s)	112, 183, 1494
Hpy99I	CGWCG	3 cut(s)	29, 53, 56
HpyAV	CCTTC	4 cut(s)	1024, 1086, 1093, 1711
HpyCH4III	ACNGT	6 cut(s)	109, 187, 340, 383, 508, 1480
HpyCH4IV	ACGT	4 cut(s)	27, 51, 177, 1189
HpyF10VI	GCNNNNNNNGC	7 cut(s)	80, 281, 618, 722, 803, 1280, 1709
HpyF3I	CTNAG	5 cut(s)	118, 350, 491, 1431, 1826
HpySE526I	ACGT	4 cut(s)	27, 51, 177, 1189
Hsp92I	GRCGYC	3 cut(s)	51, 72, 1189
Hsp92II	CATG	8 cut(s)	554, 853, 1099, 1154, 1302, 1388, 1501, 1574
HspAI	GCGC	3 cut(s)	72, 933, 1135
KasI	GGCGCC	1 cut(s)	71
KroI	GCCGGC	2 cut(s)	37, 1327
KroNI	GCCGGC	2 cut(s)	39, 1329
Ksp22I	TGATCA	1 cut(s)	1590
Kzo9I	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
LmnI	GCTCC	4 cut(s)	232, 374, 1400, 1678
Lsp1109I	GCAGC	8 cut(s)	69, 242, 245, 605, 700, 715, 1057, 1789
LweI	GCATC	6 cut(s)	112, 485, 535, 793, 946, 1612
MaeI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
MaeII	ACGT	4 cut(s)	27, 51, 177, 1189
MaeIII	GTNAC	7 cut(s)	169, 194, 328, 468, 508, 1057, 1474
MalI	GATC	9 cut(s)	660, 1053, 1168, 1172, 1366, 1502, 1568, 1592, 1876
MboI	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
MboII	GAAGA	9 cut(s)	443, 554, 1033, 1519, 1528, 1685, 1726, 1893, 1896
MfeI	CAATTG	2 cut(s)	1035, 1554
MflI	RGATCY	1 cut(s)	658
MhlI	GDGCHC	6 cut(s)	237, 243, 351, 882, 923, 1117
MluCI	AATT	6 cut(s)	570, 633, 1035, 1322, 1554, 1893
Mly113I	GGCGCC	1 cut(s)	72
MlyI	GAGTC	4 cut(s)	147, 321, 956, 1289
MmeI	TCCRAC	3 cut(s)	391, 982, 1378
Mph1103I	ATGCAT	1 cut(s)	961
MroNI	GCCGGC	2 cut(s)	37, 1327
MroXI	GAANNNNTTC	1 cut(s)	1103
MseI	TTAA	5 cut(s)	201, 480, 707, 1227, 1305
MslI	CAYNNNNRTG	2 cut(s)	1739, 1755
MspR9I	CCNGG	8 cut(s)	192, 237, 238, 277, 656, 1291, 1472, 1708
MunI	CAATTG	2 cut(s)	1035, 1554
MvaI	CCWGG	2 cut(s)	192, 1472
MvnI	CGCG	3 cut(s)	230, 307, 1056
MwoI	GCNNNNNNNGC	7 cut(s)	80, 281, 618, 722, 803, 1280, 1709
NaeI	GCCGGC	2 cut(s)	39, 1329
NarI	GGCGCC	1 cut(s)	72
NciI	CCSGG	6 cut(s)	237, 238, 277, 656, 1291, 1708
NcoI	CCATGG	1 cut(s)	1384
NdeII	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
NgoMIV	GCCGGC	2 cut(s)	37, 1327
NlaIII	CATG	8 cut(s)	554, 853, 1099, 1154, 1302, 1388, 1501, 1574
NlaIV	GGNNCC	7 cut(s)	19, 73, 234, 418, 660, 774, 1579
NmeAIII	GCCGAG	1 cut(s)	961
NmuCI	GTSAC	4 cut(s)	194, 508, 1057, 1474
NsiI	ATGCAT	1 cut(s)	961
PagI	TCATGA	3 cut(s)	550, 1095, 1150
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PdiI	GCCGGC	2 cut(s)	39, 1329
PdmI	GAANNNNTTC	1 cut(s)	1103
PfeI	GAWTC	2 cut(s)	388, 448
PleI	GAGTC	4 cut(s)	147, 320, 955, 1289
PluTI	GGCGCC	1 cut(s)	75
PpsI	GAGTC	4 cut(s)	147, 320, 955, 1289
PpuMI	RGGWCCY	1 cut(s)	416
Psp124BI	GAGCTC	1 cut(s)	1117
Psp5II	RGGWCCY	1 cut(s)	416
Psp6I	CCWGG	2 cut(s)	190, 1470
PspEI	GGTNACC	1 cut(s)	468
PspGI	CCWGG	2 cut(s)	190, 1470
PspN4I	GGNNCC	7 cut(s)	19, 73, 234, 418, 660, 774, 1579
PspPI	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
PspPPI	RGGWCCY	1 cut(s)	416
PsrI	GAACNNNNNNTAC	2 cut(s)	1589, 1621
PstI	CTGCAG	2 cut(s)	733, 955
PstNI	CAGNNNCTG	1 cut(s)	1318
PsuI	RGATCY	1 cut(s)	658
RsaI	GTAC	1 cut(s)	281
RsaNI	GTAC	1 cut(s)	280
RseI	CAYNNNNRTG	2 cut(s)	1739, 1755
SacI	GAGCTC	1 cut(s)	1117
SaqAI	TTAA	5 cut(s)	201, 480, 707, 1227, 1305
Sau3AI	GATC	9 cut(s)	658, 1051, 1166, 1170, 1364, 1500, 1566, 1590, 1874
Sau96I	GGNCC	6 cut(s)	416, 773, 974, 1073, 1577, 1663
SchI	GAGTC	4 cut(s)	147, 321, 956, 1289
ScrFI	CCNGG	8 cut(s)	192, 237, 238, 277, 656, 1291, 1472, 1708
SduI	GDGCHC	6 cut(s)	237, 243, 351, 882, 923, 1117
SfaNI	GCATC	6 cut(s)	112, 485, 535, 793, 946, 1612
SfcI	CTRYAG	4 cut(s)	729, 951, 991, 1159
SfoI	GGCGCC	1 cut(s)	73
SinI	GGWCC	4 cut(s)	416, 974, 1577, 1663
SmaI	CCCGGG	1 cut(s)	238
SmiMI	CAYNNNNRTG	2 cut(s)	1739, 1755
SmlI	CTYRAG	2 cut(s)	1130, 1419
SmoI	CTYRAG	2 cut(s)	1130, 1419
SpeI	ACTAGT	1 cut(s)	1615
SrfI	GCCCGGGC	1 cut(s)	238
Sse9I	AATT	6 cut(s)	570, 633, 1035, 1322, 1554, 1893
SsiI	CCGC	9 cut(s)	148, 230, 307, 567, 579, 725, 1338, 1545, 1813
SspDI	GGCGCC	1 cut(s)	71
SspMI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
SstI	GAGCTC	1 cut(s)	1117
StyD4I	CCNGG	8 cut(s)	190, 235, 236, 275, 654, 1289, 1470, 1706
StyI	CCWWGG	2 cut(s)	1384, 1659
TaaI	ACNGT	6 cut(s)	109, 187, 340, 383, 508, 1480
TaiI	ACGT	4 cut(s)	30, 54, 180, 1192
TaqI	TCGA	6 cut(s)	24, 216, 425, 784, 1169, 1873
TaqII	GACCGA	1 cut(s)	991
TasI	AATT	6 cut(s)	570, 633, 1035, 1322, 1554, 1893
TauI	GCSGC	2 cut(s)	728, 1815
TfiI	GAWTC	2 cut(s)	388, 448
Tru1I	TTAA	5 cut(s)	201, 480, 707, 1227, 1305
Tru9I	TTAA	5 cut(s)	201, 480, 707, 1227, 1305
TscAI	CASTG	8 cut(s)	100, 190, 343, 358, 388, 756, 1615, 1787
TseFI	GTSAC	4 cut(s)	194, 508, 1057, 1474
TseI	GCWGC	8 cut(s)	57, 255, 258, 618, 713, 728, 1045, 1802
Tsp45I	GTSAC	4 cut(s)	194, 508, 1057, 1474
TspDTI	ATGAA	8 cut(s)	567, 883, 1068, 1112, 1389, 1441, 1723, 1844
TspGWI	ACGGA	3 cut(s)	657, 1590, 1655
TspMI	CCCGGG	1 cut(s)	236
TspRI	CASTG	8 cut(s)	100, 190, 343, 358, 388, 756, 1615, 1787
VpaK11BI	GGWCC	4 cut(s)	416, 974, 1577, 1663
XapI	RAATTY	1 cut(s)	633
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1358
XmaI	CCCGGG	1 cut(s)	236
XmnI	GAANNNNTTC	1 cut(s)	1103
XspI	CTAG	5 cut(s)	300, 818, 828, 1616, 1791
ZraI	GACGTC	2 cut(s)	52, 1190
Zsp2I	ATGCAT	1 cut(s)	961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.