pycom05g03590

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006934
Physical Location & Seq
Forward (+)
4148747 .. 4151788
3042 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGGCAAGCAAAAAAATCACTTGCCCTAGTTGCAAGCAATGGGTTCAAATGACGCTGTATGCCACACAGCACCGATGCGTCTGCGGTCATGTAATCACGACTCGCAGTGCTGCTGCAGAAACACAACGAGAAACTAGTATTCATGGTCATGGAGATCAGAAACGTGAGCGTCATAGCTTGCGGAGACGGTTTGGTAGGTGTTTTCAAACCAAGGAATCATCATCAAACAATTCTTCTAGTACTTGTAGTTCAAGTCGTAATAGTTTCAAGCAATCAGGGACAGGGACAGGGACGCGGATGTCGTTGCCCTCCTCCAGCTCGGAGGAGCAAGTACCCAGTGGATCACGAGCAAACAAGCGTGCACTTCTTTGCGGGGTGACATACAAGCAACATAAATACAAGCTCAAAGGCACTTATAATGATGTGAAAAACATGGAAAACTTGCTGACTAAGACCTTCGGTTTTCCTGCCAACTGCATACGCATACTTTCAGAAGATGATCGATCGCTAGACCTAGAGCAGATTCCTACAAAGAAAAATATAGAAAATTCCTTGAAATGGCTTGTGGAGGGATGCCAGCGGGGAGACTCGTTGGTGTTTTACTTCTCGGGGCATGGCCTACGACAGCCTGATTTCAAAGACGATGAGCTTGATGGGTTCGACGAGACCATTTGCCCGGTTGATTTTATGAAAGAGGGAATGATCCTTGACAATGATATTAATGAAACCATTGTTTGGCCACTCGAGGATGGTGTCACACTTCACGCTATTGTTGATGCTTGTCACAGTGGAACGATTCTTGATCTGGAGCATGTGTATGACCGTAAACTGAATGCTTGGAAAGATAATAGTCCTCCATCTCGTGCTAGAAAAAGTACAAAGGGTGGGTTAGCGATTTCTCTTAGTGCTTGCGAAGATGATAAAATGGCAGCTGATACTACTGCTTTTGCTGGAAAATCAATGAATGGTGCAATGACATACATTCTCACTGAAACTGTTAGGAACAAGACCGACATAACATATGGAGATTTGCTCGACGAGATGGTGAAGTACATTAAAAAGATTAACAGCGAAAGATGGACTAACTCAAGATTCTTGAGGACTGTACTTCGACACAAAACCATACAGGAACCTATGTTGTCATCTTCTGAAACGTTTGATGTTAATTTGAAGAAATTTGTCTTGTAA

Protein Analysis

396

Amino Acids

44.5

Weight (kDa)

8.71

Isoelectric Point (pI)

52.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 119 - 383 1.2e-32 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AasI GACNNNNNNGTC 1 cut(s) 298
AccB7I CCANNNNNTGG 1 cut(s) 735
AccII CGCG 1 cut(s) 295
AciI CCGC 5 cut(s) 84, 181, 295, 372, 580
AclI AACGTT 1 cut(s) 1154
AclWI GGATC 2 cut(s) 349, 697
AcoI YGGCCR 1 cut(s) 737
AcsI RAATTY 2 cut(s) 547, 1175
AfaI GTAC 5 cut(s) 241, 333, 877, 1052, 1107
AfiI CCNNNNNNNGG 2 cut(s) 558, 735
AgsI TTSAA 7 cut(s) 47, 206, 252, 268, 556, 637, 1171
AhlI ACTAGT 1 cut(s) 134
AluBI AGCT 5 cut(s) 177, 318, 403, 649, 932
AluI AGCT 5 cut(s) 177, 318, 403, 649, 932
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 3 cut(s) 178, 579, 659
Alw44I GTGCAC 1 cut(s) 360
AlwI GGATC 2 cut(s) 349, 697
Ama87I CYCGRG 2 cut(s) 607, 743
AoxI GGCC 2 cut(s) 616, 737
ApaLI GTGCAC 1 cut(s) 360
ApeKI GCWGC 3 cut(s) 110, 113, 929
ApoI RAATTY 2 cut(s) 547, 1175
AseI ATTAAT 1 cut(s) 720
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 2 cut(s) 388, 1057
AvaI CYCGRG 2 cut(s) 607, 743
BaeGI GKGCMC 1 cut(s) 364
BalI TGGCCA 1 cut(s) 739
BauI CACGAG 2 cut(s) 345, 861
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 3 cut(s) 97, 100, 941
BccI CCATC 5 cut(s) 647, 743, 865, 1036, 1071
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BcnI CCSGG 1 cut(s) 677
BcoDI GTCTC 3 cut(s) 178, 579, 659
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 6 cut(s) 27, 135, 237, 509, 515, 867
BfmI CTRYAG 1 cut(s) 114
BisI GCNGC 3 cut(s) 111, 114, 930
BlsI GCNGC 3 cut(s) 112, 115, 931
BmcAI AGTACT 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 677
BmeT110I CYCGRG 2 cut(s) 607, 743
BmiI GGNNCC 1 cut(s) 1131
BmrFI CCNGG 1 cut(s) 677
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 3 cut(s) 65, 563, 766
BmuI ACTGGG 1 cut(s) 330
BplI GAGNNNNNCTC 2 cut(s) 1017, 1049
BpmI CTGGAG 2 cut(s) 298, 827
BpuEI CTTGAG 2 cut(s) 1072, 1117
BpuMI CCSGG 1 cut(s) 677
Bsa29I ATCGAT 1 cut(s) 502
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 1 cut(s) 210
BsaXI ACNNNNNCTCC 2 cut(s) 800, 830
Bsc4I CCNNNNNNNGG 2 cut(s) 558, 735
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 2 cut(s) 44, 978
BseCI ATCGAT 1 cut(s) 502
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 3 cut(s) 303, 578, 754
BseLI CCNNNNNNNGG 2 cut(s) 558, 735
BseMI GCAATG 2 cut(s) 44, 978
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 2 cut(s) 301, 338
BseSI GKGCMC 1 cut(s) 364
BseXI GCAGC 3 cut(s) 97, 100, 941
Bsh1236I CGCG 1 cut(s) 295
Bsh1285I CGRYCG 1 cut(s) 506
BshFI GGCC 2 cut(s) 618, 739
BshVI ATCGAT 1 cut(s) 502
BsiEI CGRYCG 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 364
BsiHKCI CYCGRG 2 cut(s) 607, 743
BsiSI CCGG 1 cut(s) 677
BslFI GGGAC 3 cut(s) 292, 298, 304
BslI CCNNNNNNNGG 2 cut(s) 558, 735
BsmAI GTCTC 3 cut(s) 178, 579, 659
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 3 cut(s) 292, 298, 304
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 2 cut(s) 618, 739
Bso31I GGTCTC 1 cut(s) 659
BsoBI CYCGRG 2 cut(s) 607, 743
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 6 cut(s) 154, 341, 499, 503, 702, 802
BspACI CCGC 5 cut(s) 84, 181, 295, 372, 580
BspANI GGCC 2 cut(s) 618, 739
BspDI ATCGAT 1 cut(s) 502
BspFNI CGCG 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 1131
BspMAI CTGCAG 1 cut(s) 118
BspPI GGATC 2 cut(s) 349, 697
BspTNI GGTCTC 1 cut(s) 659
BsrDI GCAATG 2 cut(s) 44, 978
BsrI ACTGG 1 cut(s) 336
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 6 cut(s) 154, 341, 499, 503, 702, 802
BssSI CACGAG 2 cut(s) 345, 861
BssT1I CCWWGG 1 cut(s) 210
Bst2BI CACGAG 2 cut(s) 345, 861
Bst4CI ACNGT 5 cut(s) 189, 788, 824, 997, 1105
BstC8I GCNNGC 6 cut(s) 7, 35, 179, 360, 578, 910
BstDEI CTNAG 2 cut(s) 450, 902
BstF5I GGATG 3 cut(s) 303, 578, 754
BstFNI CGCG 1 cut(s) 295
BstKTI GATC 6 cut(s) 157, 344, 502, 506, 705, 805
BstMAI GTCTC 3 cut(s) 178, 579, 659
BstMBI GATC 6 cut(s) 154, 341, 499, 503, 702, 802
BstMCI CGRYCG 1 cut(s) 506
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 815
BstSCI CCNGG 1 cut(s) 675
BstSFI CTRYAG 1 cut(s) 114
BstSLI GKGCMC 1 cut(s) 364
BstUI CGCG 1 cut(s) 295
BstV1I GCAGC 3 cut(s) 97, 100, 941
Bsu15I ATCGAT 1 cut(s) 502
BsuRI GGCC 2 cut(s) 618, 739
BsuTUI ATCGAT 1 cut(s) 502
BtsCI GGATG 3 cut(s) 303, 578, 754
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 4 cut(s) 112, 343, 793, 987
Cac8I GCNNGC 6 cut(s) 7, 35, 179, 360, 578, 910
ClaI ATCGAT 1 cut(s) 502
CseI GACGC 4 cut(s) 61, 67, 158, 301
Csp6I GTAC 5 cut(s) 240, 332, 876, 1051, 1106
CviAII CATG 6 cut(s) 89, 143, 149, 433, 614, 812
CviJI RGCY 9 cut(s) 177, 318, 403, 562, 618, 628, 649, 739, 932
CviKI_1 RGCY 9 cut(s) 177, 318, 403, 562, 618, 628, 649, 739, 932
CviQI GTAC 5 cut(s) 240, 332, 876, 1051, 1106
DdeI CTNAG 2 cut(s) 450, 902
DpnI GATC 6 cut(s) 156, 343, 501, 505, 704, 804
DpnII GATC 6 cut(s) 154, 341, 499, 503, 702, 802
DrdI GACNNNNNNGTC 1 cut(s) 298
DseDI GACNNNNNNGTC 1 cut(s) 298
EaeI YGGCCR 1 cut(s) 737
Eco130I CCWWGG 1 cut(s) 210
Eco31I GGTCTC 1 cut(s) 659
Eco88I CYCGRG 2 cut(s) 607, 743
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 178
FaeI CATG 6 cut(s) 92, 146, 152, 436, 617, 815
FaqI GGGAC 3 cut(s) 292, 298, 304
FatI CATG 6 cut(s) 88, 142, 148, 432, 613, 811
FauI CCCGC 2 cut(s) 365, 573
FauNDI CATATG 1 cut(s) 1021
Fnu4HI GCNGC 3 cut(s) 111, 114, 930
FokI GGATG 3 cut(s) 310, 585, 761
Fsp4HI GCNGC 3 cut(s) 111, 114, 930
FspBI CTAG 6 cut(s) 27, 135, 237, 509, 515, 867
GluI GCNGC 3 cut(s) 111, 114, 930
GsuI CTGGAG 2 cut(s) 298, 827
HaeIII GGCC 2 cut(s) 618, 739
HapII CCGG 1 cut(s) 677
HgaI GACGC 4 cut(s) 61, 67, 158, 301
Hin1II CATG 6 cut(s) 92, 146, 152, 436, 617, 815
HinfI GANTC 6 cut(s) 100, 215, 523, 587, 796, 1092
HpaII CCGG 1 cut(s) 677
HphI GGTGA 2 cut(s) 388, 1057
Hpy166II GTNNAC 2 cut(s) 362, 827
Hpy188I TCNGA 4 cut(s) 159, 322, 493, 1150
Hpy188III TCNNGA 6 cut(s) 97, 345, 800, 806, 1089, 1096
Hpy8I GTNNAC 2 cut(s) 362, 827
Hpy99I CGWCG 2 cut(s) 665, 1040
HpyAV CCTTC 1 cut(s) 466
HpyCH4III ACNGT 5 cut(s) 189, 788, 824, 997, 1105
HpyCH4IV ACGT 2 cut(s) 163, 1154
HpyCH4V TGCA 5 cut(s) 33, 116, 362, 477, 971
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 2 cut(s) 450, 902
HpySE526I ACGT 2 cut(s) 163, 1154
Hsp92II CATG 6 cut(s) 92, 146, 152, 436, 617, 815
Kzo9I GATC 6 cut(s) 154, 341, 499, 503, 702, 802
LmnI GCTCC 2 cut(s) 325, 808
Lsp1109I GCAGC 3 cut(s) 97, 100, 941
LweI GCATC 3 cut(s) 65, 563, 766
MaeI CTAG 6 cut(s) 27, 135, 237, 509, 515, 867
MaeII ACGT 2 cut(s) 163, 1154
MaeIII GTNAC 3 cut(s) 376, 754, 782
MalI GATC 6 cut(s) 156, 343, 501, 505, 704, 804
MboI GATC 6 cut(s) 154, 341, 499, 503, 702, 802
MboII GAAGA 5 cut(s) 225, 506, 926, 1137, 1183
MhlI GDGCHC 1 cut(s) 364
MlsI TGGCCA 1 cut(s) 739
MluCI AATT 4 cut(s) 229, 547, 1165, 1175
MluNI TGGCCA 1 cut(s) 739
MlyI GAGTC 2 cut(s) 94, 581
MnlI CCTC 8 cut(s) 316, 319, 322, 562, 688, 739, 864, 1092
Mox20I TGGCCA 1 cut(s) 739
MscI TGGCCA 1 cut(s) 739
MseI TTAA 4 cut(s) 720, 1056, 1065, 1164
MslI CAYNNNNRTG 2 cut(s) 147, 816
Msp20I TGGCCA 1 cut(s) 739
MspA1I CMGCKG 2 cut(s) 580, 932
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 1 cut(s) 677
Mva1269I GAATGC 1 cut(s) 838
MvnI CGCG 1 cut(s) 295
MwoI GCNNNNNNNGC 1 cut(s) 30
NciI CCSGG 1 cut(s) 677
NdeI CATATG 1 cut(s) 1021
NdeII GATC 6 cut(s) 154, 341, 499, 503, 702, 802
NlaIII CATG 6 cut(s) 92, 146, 152, 436, 617, 815
NlaIV GGNNCC 1 cut(s) 1131
NmuCI GTSAC 3 cut(s) 376, 754, 782
NspI RCATGY 1 cut(s) 815
PaeR7I CTCGAG 1 cut(s) 743
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PctI GAATGC 1 cut(s) 838
PfeI GAWTC 4 cut(s) 215, 523, 796, 1092
PflMI CCANNNNNTGG 1 cut(s) 735
PkrI GCNGC 3 cut(s) 112, 115, 931
Ple19I CGATCG 1 cut(s) 506
PleI GAGTC 2 cut(s) 94, 581
PpsI GAGTC 2 cut(s) 94, 581
PshBI ATTAAT 1 cut(s) 720
PsiI TTATAA 1 cut(s) 417
Psp1406I AACGTT 1 cut(s) 1154
PspN4I GGNNCC 1 cut(s) 1131
PspXI VCTCGAGB 1 cut(s) 743
PsrI GAACNNNNNNTAC 2 cut(s) 232, 264
PstI CTGCAG 1 cut(s) 118
PvuI CGATCG 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 932
RsaI GTAC 5 cut(s) 241, 333, 877, 1052, 1107
RsaNI GTAC 5 cut(s) 240, 332, 876, 1051, 1106
RseI CAYNNNNRTG 2 cut(s) 147, 816
SaqAI TTAA 4 cut(s) 720, 1056, 1065, 1164
SatI GCNGC 3 cut(s) 111, 114, 930
Sau3AI GATC 6 cut(s) 154, 341, 499, 503, 702, 802
ScaI AGTACT 1 cut(s) 241
SchI GAGTC 2 cut(s) 94, 581
ScrFI CCNGG 1 cut(s) 677
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 3 cut(s) 65, 563, 766
SfcI CTRYAG 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 743
SlaI CTCGAG 1 cut(s) 743
SmiMI CAYNNNNRTG 2 cut(s) 147, 816
SmlI CTYRAG 3 cut(s) 743, 1087, 1096
SmoI CTYRAG 3 cut(s) 743, 1087, 1096
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 4 cut(s) 229, 547, 1165, 1175
SsiI CCGC 5 cut(s) 84, 181, 295, 372, 580
SspMI CTAG 6 cut(s) 27, 135, 237, 509, 515, 867
StyD4I CCNGG 1 cut(s) 675
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 5 cut(s) 189, 788, 824, 997, 1105
TaiI ACGT 2 cut(s) 166, 1157
TaqI TCGA 5 cut(s) 502, 660, 744, 1035, 1111
TaqII GACCGA 1 cut(s) 1025
TasI AATT 4 cut(s) 229, 547, 1165, 1175
TatI WGTACW 4 cut(s) 239, 875, 1050, 1105
TfiI GAWTC 4 cut(s) 215, 523, 796, 1092
Tru1I TTAA 4 cut(s) 720, 1056, 1065, 1164
Tru9I TTAA 4 cut(s) 720, 1056, 1065, 1164
TscAI CASTG 4 cut(s) 112, 343, 793, 994
TseFI GTSAC 3 cut(s) 376, 754, 782
TseI GCWGC 3 cut(s) 110, 113, 929
Tsp45I GTSAC 3 cut(s) 376, 754, 782
TspDTI ATGAA 4 cut(s) 131, 704, 738, 977
TspRI CASTG 4 cut(s) 112, 343, 793, 994
Van91I CCANNNNNTGG 1 cut(s) 735
VneI GTGCAC 1 cut(s) 360
VspI ATTAAT 1 cut(s) 720
XapI RAATTY 2 cut(s) 547, 1175
XceI RCATGY 1 cut(s) 815
XhoI CTCGAG 1 cut(s) 743
XspI CTAG 6 cut(s) 27, 135, 237, 509, 515, 867
ZrmI AGTACT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.