pycom05g10340

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005175
Physical Location & Seq
Reverse (-)
13714770 .. 13715458
689 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGAAATATTCACGATCCTTGCTCTTTGGTGTGCTGCTACTCATTGGCTTTGCATTGACAAATAGCAAAGGAACTAATACGGATCCACCTGTTCTTTGCGAATTTCTCAATCGCAGCAGTTTTGAACCAGGGTTCATATTTGGCTCAGGTTCAGCATCTTACCAGGTTAGTATATATATTACAGGTTTAAAGCCGTTTGTAACACTCTTTCATTGGGATGTTCCCCAAGCTTTAGAAGATGAATATGGTGGTTTCTTAAGCCCTCGTATTGTCGATCATTTTAAAGACTATGTAGAACTTTGTTATAAAGAATTTGGTGATCGAGTCAAGCACTGGTTCACGTTAAATGAGCCGCATACTTTTTGTAATATGGGTTATGCTCTTGGGAGTTACGCACCGGGACGCTGCTCTTCTTGGCAAAACCTAAACTGCACCGGTGGAGATTCAGCCATTGAACCATACTTGGTGGCACACCACCTTCTTCTTGCTCATGGAGCAGCTGTAGAATTGTACAAGAACAGATATCAGGTTCAATAA

Protein Analysis

179

Amino Acids

20.05

Weight (kDa)

6.13

Isoelectric Point (pI)

27.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 61 - 176 2.3e-35 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 306
AciI CCGC 1 cut(s) 353
AclWI GGATC 3 cut(s) 9, 77, 90
AcsI RAATTY 2 cut(s) 101, 311
AfaI GTAC 1 cut(s) 512
AflII CTTAAG 1 cut(s) 256
AgeI ACCGGT 1 cut(s) 434
AgsI TTSAA 3 cut(s) 125, 455, 533
AjnI CCWGG 2 cut(s) 127, 162
AluBI AGCT 2 cut(s) 230, 500
AluI AGCT 2 cut(s) 230, 500
AlwI GGATC 3 cut(s) 9, 77, 90
ApeKI GCWGC 4 cut(s) 34, 114, 405, 497
ApoI RAATTY 2 cut(s) 101, 311
AsiGI ACCGGT 1 cut(s) 434
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 1 cut(s) 329
BamHI GGATCC 1 cut(s) 82
BbvI GCAGC 4 cut(s) 21, 126, 392, 509
BceAI ACGGC 1 cut(s) 178
BciT130I CCWGG 2 cut(s) 129, 164
BcnI CCSGG 1 cut(s) 399
BfmI CTRYAG 1 cut(s) 501
BfrI CTTAAG 1 cut(s) 256
BisI GCNGC 5 cut(s) 35, 115, 353, 406, 498
BlsI GCNGC 5 cut(s) 36, 116, 354, 407, 499
Bme1390I CCNGG 3 cut(s) 129, 164, 399
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 3 cut(s) 129, 164, 399
BmsI GCATC 1 cut(s) 164
Bpu10I CCTNAGC 1 cut(s) 145
BpuMI CCSGG 1 cut(s) 399
BsaJI CCNNGG 1 cut(s) 128
BsaWI WCCGGW 1 cut(s) 434
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 338
BseBI CCWGG 2 cut(s) 129, 164
BseDI CCNNGG 1 cut(s) 128
BseGI GGATG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 1 cut(s) 338
BseXI GCAGC 4 cut(s) 21, 126, 392, 509
BsgI GTGCAG 1 cut(s) 415
BshTI ACCGGT 1 cut(s) 434
BsiSI CCGG 2 cut(s) 398, 435
BslFI GGGAC 1 cut(s) 414
BsmFI GGGAC 1 cut(s) 414
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 4 cut(s) 14, 82, 274, 319
BspACI CCGC 1 cut(s) 353
BspCNI CTCAG 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 84
BspPI GGATC 3 cut(s) 9, 77, 90
BspQI GCTCTTC 1 cut(s) 415
BspTI CTTAAG 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 434
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 1 cut(s) 338
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 1 cut(s) 128
BssMI GATC 4 cut(s) 14, 82, 274, 319
Bst2UI CCWGG 2 cut(s) 129, 164
Bst6I CTCTTC 1 cut(s) 415
BstAFI CTTAAG 1 cut(s) 256
BstAUI TGTACA 1 cut(s) 510
BstDEI CTNAG 1 cut(s) 145
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 4 cut(s) 17, 85, 277, 322
BstMBI GATC 4 cut(s) 14, 82, 274, 319
BstMWI GCNNNNNNNGC 1 cut(s) 494
BstNI CCWGG 2 cut(s) 129, 164
BstSCI CCNGG 3 cut(s) 127, 162, 397
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 4 cut(s) 21, 126, 392, 509
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BtsCI GGATG 1 cut(s) 223
BtsIMutI CAGTG 1 cut(s) 331
Cfr10I RCCGGY 1 cut(s) 434
CseI GACGC 1 cut(s) 411
CsiI ACCWGGT 1 cut(s) 162
Csp6I GTAC 1 cut(s) 511
CspAI ACCGGT 1 cut(s) 434
CviAII CATG 1 cut(s) 491
CviJI RGCY 8 cut(s) 48, 144, 193, 230, 261, 352, 449, 500
CviKI_1 RGCY 8 cut(s) 48, 144, 193, 230, 261, 352, 449, 500
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 1 cut(s) 145
DpnI GATC 4 cut(s) 16, 84, 276, 321
DpnII GATC 4 cut(s) 14, 82, 274, 319
DraI TTTAAA 2 cut(s) 189, 283
Eam1104I CTCTTC 1 cut(s) 415
EarI CTCTTC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 524
EcoRII CCWGG 2 cut(s) 127, 162
EcoRV GATATC 1 cut(s) 524
FaeI CATG 1 cut(s) 494
FaqI GGGAC 1 cut(s) 414
FatI CATG 1 cut(s) 490
Fnu4HI GCNGC 5 cut(s) 35, 115, 353, 406, 498
FokI GGATG 1 cut(s) 230
Fsp4HI GCNGC 5 cut(s) 35, 115, 353, 406, 498
GluI GCNGC 5 cut(s) 35, 115, 353, 406, 498
HapII CCGG 2 cut(s) 398, 435
HgaI GACGC 1 cut(s) 411
Hin1II CATG 1 cut(s) 494
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 2 cut(s) 324, 443
HpaII CCGG 2 cut(s) 398, 435
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 339
Hpy188III TCNNGA 1 cut(s) 12
Hpy8I GTNNAC 1 cut(s) 339
HpyAV CCTTC 1 cut(s) 488
HpyCH4IV ACGT 1 cut(s) 341
HpyCH4V TGCA 2 cut(s) 53, 432
HpyF10VI GCNNNNNNNGC 1 cut(s) 494
HpyF3I CTNAG 1 cut(s) 145
HpySE526I ACGT 1 cut(s) 341
Hsp92II CATG 1 cut(s) 494
Kzo9I GATC 4 cut(s) 14, 82, 274, 319
LguI GCTCTTC 1 cut(s) 415
LmnI GCTCC 1 cut(s) 494
Lsp1109I GCAGC 4 cut(s) 21, 126, 392, 509
LweI GCATC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 162
MaeII ACGT 1 cut(s) 341
MaeIII GTNAC 2 cut(s) 199, 389
MalI GATC 4 cut(s) 16, 84, 276, 321
MboI GATC 4 cut(s) 14, 82, 274, 319
MboII GAAGA 3 cut(s) 248, 402, 473
MflI RGATCY 1 cut(s) 82
MluCI AATT 3 cut(s) 101, 311, 506
MlyI GAGTC 1 cut(s) 333
MnlI CCTC 1 cut(s) 273
MseI TTAA 4 cut(s) 188, 257, 282, 344
MslI CAYNNNNRTG 1 cut(s) 216
MspA1I CMGCKG 1 cut(s) 500
MspCI CTTAAG 1 cut(s) 256
MspI CCGG 2 cut(s) 398, 435
MspR9I CCNGG 3 cut(s) 129, 164, 399
MvaI CCWGG 2 cut(s) 129, 164
MwoI GCNNNNNNNGC 1 cut(s) 494
NciI CCSGG 1 cut(s) 399
NdeII GATC 4 cut(s) 14, 82, 274, 319
NlaIII CATG 1 cut(s) 494
NlaIV GGNNCC 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 415
PfeI GAWTC 1 cut(s) 443
PinAI ACCGGT 1 cut(s) 434
PkrI GCNGC 5 cut(s) 36, 116, 354, 407, 499
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
PsiI TTATAA 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 127, 162
PspGI CCWGG 2 cut(s) 127, 162
PspN4I GGNNCC 1 cut(s) 84
PsuI RGATCY 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
RseI CAYNNNNRTG 1 cut(s) 216
SapI GCTCTTC 1 cut(s) 415
SaqAI TTAA 4 cut(s) 188, 257, 282, 344
SatI GCNGC 5 cut(s) 35, 115, 353, 406, 498
Sau3AI GATC 4 cut(s) 14, 82, 274, 319
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 3 cut(s) 129, 164, 399
SexAI ACCWGGT 1 cut(s) 162
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 501
SgrAI CRCCGGYG 1 cut(s) 434
SmiMI CAYNNNNRTG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 3 cut(s) 101, 311, 506
SsiI CCGC 1 cut(s) 353
SspI AATATT 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 127, 162, 397
TaiI ACGT 1 cut(s) 344
TaqI TCGA 2 cut(s) 273, 322
TasI AATT 3 cut(s) 101, 311, 506
TatI WGTACW 1 cut(s) 510
TauI GCSGC 1 cut(s) 355
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 4 cut(s) 188, 257, 282, 344
Tru9I TTAA 4 cut(s) 188, 257, 282, 344
TscAI CASTG 1 cut(s) 338
TseI GCWGC 4 cut(s) 34, 114, 405, 497
TspDTI ATGAA 4 cut(s) 17, 124, 200, 255
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 1 cut(s) 338
Vha464I CTTAAG 1 cut(s) 256
XapI RAATTY 2 cut(s) 101, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.