RchiOBHm_Chr5g0077931

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005175
Physical Location & Seq
Reverse (-)
83792934 .. 83796827
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35248

Sequence Viewer

Length: 693 bp
ATGAAAAATATGGGCTTTGAGTTCTCCCTCTCGTTATCTAGATTGTTACCAAATAAGACATTCAAATCGATTACATCACCACCCTGTCTAAGGCCCTTTGTCACACTCTTCCATTGGGATCTTCCCCAAGCTTTAGAAGGTGAATACGATGGCTTCTTAAGCCCTCAAATTGTCTATGCTTCAAGGAATTTGGTGATAGAGTTAAGGACTGGAACGAGCCCTGGAACTACGTATGCCAATGGTGGTTATGTAATTGGGACTTTTGCACCCTGCCAAAGTTTTGAATGGCATTGTACCAGTGGAAATTCAGGAACAGAGCCATATTTTGTCTCACACTATCAGCTTCTAGCTCACGCAGCAGCTGTAAAGTTATACAAGGAGAAGTACCGGGAAATGTGGGTGGGAGGTGGTAGTTGGGAGACAGAAGAGGTTGGGGGTTTTGGAGAGATGTATAGAGACGGTTTAGAGTCAAAACCGATCCAACCTAATCCTTGTGCAGCCCTAGTGATAGCGATAACAATATTGTCGCACTGGTTTGTGCCCTTTTCTGATGCCAAGCACCATGAAGAAGCTGCTATACGAGCATTGGATTTTATGTTTGGATGGTATGTAACAATTTTTAAATACCAATTAATTAAAGTGTGTGCACACACTTTCCATATACCATGTGGCATATGGAAAGTACTTGTCTAA

Protein Analysis

230

Amino Acids

25.96

Weight (kDa)

6.49

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 30 - 135 9.4e-12 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 126, 472
AcsI RAATTY 2 cut(s) 187, 304
AfaI GTAC 3 cut(s) 295, 386, 684
AfiI CCNNNNNNNGG 1 cut(s) 90
AflII CTTAAG 1 cut(s) 157
AgsI TTSAA 3 cut(s) 64, 183, 284
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 5 cut(s) 131, 343, 350, 362, 572
AluI AGCT 5 cut(s) 131, 343, 350, 362, 572
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 3 cut(s) 334, 413, 450
Alw44I GTGCAC 1 cut(s) 645
AlwI GGATC 2 cut(s) 126, 472
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 1 cut(s) 92
ApaLI GTGCAC 1 cut(s) 645
ApeKI GCWGC 4 cut(s) 356, 359, 497, 572
ApoI RAATTY 2 cut(s) 187, 304
AseI ATTAAT 1 cut(s) 632
AspS9I GGNCC 1 cut(s) 93
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 3 cut(s) 69, 152, 205
BaeGI GKGCMC 2 cut(s) 543, 649
BanII GRGCYC 1 cut(s) 221
BarI GAAGNNNNNNTAC 2 cut(s) 561, 593
Bbv12I GWGCWC 1 cut(s) 649
BbvI GCAGC 4 cut(s) 368, 371, 509, 559
BccI CCATC 2 cut(s) 143, 597
BciT130I CCWGG 1 cut(s) 222
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 3 cut(s) 334, 413, 450
BfaI CTAG 3 cut(s) 39, 347, 503
BfrI CTTAAG 1 cut(s) 157
BisI GCNGC 4 cut(s) 357, 360, 498, 573
BlsI GCNGC 4 cut(s) 358, 361, 499, 574
BmcAI AGTACT 1 cut(s) 684
Bme1390I CCNGG 2 cut(s) 222, 389
BmgT120I GGNCC 1 cut(s) 93
BmrFI CCNGG 2 cut(s) 222, 389
BmsI GCATC 1 cut(s) 541
BpuMI CCSGG 1 cut(s) 389
Bsa29I ATCGAT 1 cut(s) 68
BsaAI YACGTR 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 90
Bse1I ACTGG 3 cut(s) 214, 297, 536
BseBI CCWGG 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 1 cut(s) 608
BseLI CCNNNNNNNGG 1 cut(s) 90
BseNI ACTGG 3 cut(s) 214, 297, 536
BseSI GKGCMC 2 cut(s) 543, 649
BseXI GCAGC 4 cut(s) 368, 371, 509, 559
BsgI GTGCAG 1 cut(s) 516
BshFI GGCC 1 cut(s) 94
BshVI ATCGAT 1 cut(s) 68
BsiHKAI GWGCWC 1 cut(s) 649
BsiSI CCGG 1 cut(s) 388
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 1 cut(s) 90
BsmAI GTCTC 3 cut(s) 334, 413, 450
BsmBI CGTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 271
BsnI GGCC 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 221, 543, 649
Bsp143I GATC 2 cut(s) 118, 477
BspANI GGCC 1 cut(s) 94
BspDI ATCGAT 1 cut(s) 68
BspPI GGATC 2 cut(s) 126, 472
BspTI CTTAAG 1 cut(s) 157
BsrI ACTGG 3 cut(s) 214, 297, 536
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 2 cut(s) 118, 477
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 2 cut(s) 113, 420
BstAFI CTTAAG 1 cut(s) 157
BstBAI YACGTR 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 89
BstENI CCTNNNNNAGG 1 cut(s) 88
BstF5I GGATG 1 cut(s) 608
BstKTI GATC 2 cut(s) 121, 480
BstMAI GTCTC 3 cut(s) 334, 413, 450
BstMBI GATC 2 cut(s) 118, 477
BstMWI GCNNNNNNNGC 3 cut(s) 159, 356, 581
BstNI CCWGG 1 cut(s) 222
BstSCI CCNGG 2 cut(s) 220, 387
BstSLI GKGCMC 2 cut(s) 543, 649
BstSNI TACGTA 1 cut(s) 231
BstV1I GCAGC 4 cut(s) 368, 371, 509, 559
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
Bsu15I ATCGAT 1 cut(s) 68
BsuRI GGCC 1 cut(s) 94
BsuTUI ATCGAT 1 cut(s) 68
BtsCI GGATG 1 cut(s) 608
BtsIMutI CAGTG 2 cut(s) 304, 529
CaiI CAGNNNCTG 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 68
Csp6I GTAC 3 cut(s) 294, 385, 683
CviAII CATG 2 cut(s) 563, 666
CviQI GTAC 3 cut(s) 294, 385, 683
DdeI CTNAG 1 cut(s) 89
DpnI GATC 2 cut(s) 120, 479
DpnII GATC 2 cut(s) 118, 477
DraI TTTAAA 1 cut(s) 622
Eam1104I CTCTTC 2 cut(s) 113, 420
EarI CTCTTC 2 cut(s) 113, 420
Eco105I TACGTA 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 221
EcoNI CCTNNNNNAGG 1 cut(s) 88
EcoO109I RGGNCCY 1 cut(s) 93
EcoRII CCWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 221
Esp3I CGTCTC 1 cut(s) 450
FaeI CATG 2 cut(s) 566, 669
FaqI GGGAC 1 cut(s) 271
FatI CATG 2 cut(s) 562, 665
FauNDI CATATG 1 cut(s) 674
Fnu4HI GCNGC 4 cut(s) 357, 360, 498, 573
FokI GGATG 1 cut(s) 615
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 4 cut(s) 357, 360, 498, 573
FspBI CTAG 3 cut(s) 39, 347, 503
GluI GCNGC 4 cut(s) 357, 360, 498, 573
HaeIII GGCC 1 cut(s) 94
HapII CCGG 1 cut(s) 388
Hin1II CATG 2 cut(s) 566, 669
HindIII AAGCTT 1 cut(s) 129
HinfI GANTC 1 cut(s) 467
HpaII CCGG 1 cut(s) 388
HphI GGTGA 3 cut(s) 69, 152, 205
Hpy166II GTNNAC 1 cut(s) 647
Hpy188I TCNGA 1 cut(s) 550
Hpy188III TCNNGA 2 cut(s) 39, 309
Hpy8I GTNNAC 1 cut(s) 647
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 3 cut(s) 266, 497, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 356, 581
HpyF3I CTNAG 1 cut(s) 89
HpySE526I ACGT 1 cut(s) 230
Hsp92II CATG 2 cut(s) 566, 669
Kzo9I GATC 2 cut(s) 118, 477
LpnPI CCDG 9 cut(s) 97, 195, 207, 234, 283, 294, 310, 401, 517
Lsp1109I GCAGC 4 cut(s) 368, 371, 509, 559
LweI GCATC 1 cut(s) 541
MaeI CTAG 3 cut(s) 39, 347, 503
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 3 cut(s) 45, 100, 610
MalI GATC 2 cut(s) 120, 479
MboI GATC 2 cut(s) 118, 477
MboII GAAGA 4 cut(s) 100, 113, 437, 578
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 3 cut(s) 221, 543, 649
MluCI AATT 7 cut(s) 168, 187, 252, 304, 615, 629, 633
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 4 cut(s) 38, 174, 398, 421
MseI TTAA 5 cut(s) 158, 203, 621, 632, 636
MspA1I CMGCKG 1 cut(s) 362
MspCI CTTAAG 1 cut(s) 157
MspI CCGG 1 cut(s) 388
MspR9I CCNGG 2 cut(s) 222, 389
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 3 cut(s) 159, 356, 581
NciI CCSGG 1 cut(s) 389
NdeI CATATG 1 cut(s) 674
NdeII GATC 2 cut(s) 118, 477
NlaIII CATG 2 cut(s) 566, 669
NmuCI GTSAC 1 cut(s) 100
PacI TTAATTAA 1 cut(s) 636
PkrI GCNGC 4 cut(s) 358, 361, 499, 574
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
Ppu21I YACGTR 1 cut(s) 231
PshBI ATTAAT 1 cut(s) 632
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspPI GGNCC 1 cut(s) 93
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 3 cut(s) 295, 386, 684
RsaNI GTAC 3 cut(s) 294, 385, 683
SaqAI TTAA 5 cut(s) 158, 203, 621, 632, 636
SatI GCNGC 4 cut(s) 357, 360, 498, 573
Sau3AI GATC 2 cut(s) 118, 477
Sau96I GGNCC 1 cut(s) 93
ScaI AGTACT 1 cut(s) 684
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 2 cut(s) 222, 389
SduI GDGCHC 3 cut(s) 221, 543, 649
SfaNI GCATC 1 cut(s) 541
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SnaBI TACGTA 1 cut(s) 231
Sse9I AATT 7 cut(s) 168, 187, 252, 304, 615, 629, 633
SspI AATATT 1 cut(s) 522
SspMI CTAG 3 cut(s) 39, 347, 503
StyD4I CCNGG 2 cut(s) 220, 387
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 1 cut(s) 233
TaqI TCGA 1 cut(s) 68
TasI AATT 7 cut(s) 168, 187, 252, 304, 615, 629, 633
TatI WGTACW 1 cut(s) 682
Tru1I TTAA 5 cut(s) 158, 203, 621, 632, 636
Tru9I TTAA 5 cut(s) 158, 203, 621, 632, 636
TscAI CASTG 2 cut(s) 304, 536
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 4 cut(s) 356, 359, 497, 572
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 2 cut(s) 17, 579
TspRI CASTG 2 cut(s) 304, 536
Vha464I CTTAAG 1 cut(s) 157
VneI GTGCAC 1 cut(s) 645
VspI ATTAAT 1 cut(s) 632
XagI CCTNNNNNAGG 1 cut(s) 88
XapI RAATTY 2 cut(s) 187, 304
XbaI TCTAGA 1 cut(s) 38
XcmI CCANNNNNNNNNTGG 2 cut(s) 665, 672
XspI CTAG 3 cut(s) 39, 347, 503
ZrmI AGTACT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.