Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005175

Physical Location & Seq

Genomic Coordinates Reverse (-)

5770038 .. 5787933

Gene Structure 17896 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00004270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGGATAGATCTCGATCTTTCAAGAAGCGATCTACTTACATGAGTGGCAAGTATAGCTACCTCCCCAAGCCATTTACGACGTTGCTTCCATCAACCGGAGCACCTTTCCGGCAGGTTTCATATTTGGGACAGCTTCTTCAGCTTACCAGTTCGAAGGTGCTGCAAAAGAAGATGGAAGAGGACCAAGCATATGGGACACTTACACTCACAACATTCCAGGAGGATGTTGGCATCATGAAAAATATGGGTTTTGATGCTTACAGATTCTCCATCTCATGGTCTAGATTGTTACCAGGTCTAAAGCCCTTTGTCACACTCTTCCATTGGGATCTTCCCCAAGCTTTAGAAGATGAATACGGTGGCTTCTTAAGCCCTCAAATTGTAAACCATTTCCAAGACTACGCAGAGCTATGCTTCAAGGAATTTGGTGATAGAGTTAAGGACTGGATCACATTAAACGAGCCCTGGAGCTACGCTAATGGTGGTTATGTAATTGGGACGTTTGCACCATGCCGATGTTCTGAATGGCAAAACCTAAATTGCACCGGTGGAAATTCAGGAACAGAGCCATATTTAGTTTCACACTACCAGCTTCTAGCTCACGCAGCAGCCGTAAAGTTATACAAGGAGAAGTATCAGGCTGATCAGAAGGGTGTGATAGGGATAACAATACTGTCACACTGGTTTGTGCCCTTTTCTGATGCCAAGCACCATGAAGAAGCTGCTCTACGAGCATTGGATTTTATGTTTGGATGTTCGAAGGGGCTGCAAAAGAAGGTGGTAGAGGACCGTGTGTATGGGACGCCTTTACTCACAAGTATCCAGGAAGATGTGAAGATGTTGAAGGACATAGGTTGGGATGCTTACAGATTCTCAATCTCATGGTCTAGACTGTTACCAAATGGAAAGCTGAGTGGGGGTGTGAACCAGGAAGGAATCAAATATTACAAGAACCTCATCAACGAGCTTCTAGCAAATGGTAGTACTCATGAGCTTATCATTATGTATAAGCCCCTACGTCACACTCTTTCATTGGGATACGCCCCAAGCTTTAGAAGAAGAATACGGTGGTTTCCTAAGCCCTCAAATTGTGCAAAACAGAAGGGTTTGATAGGGATAACGGTAAACACAGGATGGTTTGTGCCATATTCCGACTCAAAGCGTGACCAAGATGCTGCACAACGAGCAATGGATTTCAACCTCGGATGTAAGAGGAGAGCAAATGTAAGAGTGGATGAGGTGAATGATCCTACATGGTCGCTTGAGAAAGCTCTTGACGATAGTAAAAGAATTGAATATTACGATCAACATCTTCAGAAACTTCATGCAGCAATTAATTCTCCAAAGCTCGCGGCGGTATTGGAACATCTTTCTCTAGGCGGCGACGATGGCACCATCGAGTTAGTTGGTGTCCGGGACGCGGGGGCTCCGGATCGGTTTGGAATCGAGTCCGGCTACCATGCTATGGTCAAGCGCCTTCTCGGTGATGGCATCGTGGTGGGGACAACGGTTTCCTCGAGTCATGGTCGTGGGGTGCACCGGTGCAAGGGAGGAGCGCACTTGCGGTGGCGGTTCGATGGAGGGTGGTTCATAGGCTCGCCGAATAAGTGTGCACTTGGTTGTCTAATGGGTGGTGCTGCTAGCATACCACGTCCTAAACTTGTCCTAGGATTGGCAAGAGCAGATGTGTTTTTATTCTTCAATAAATGTGATGGCAAGACTGCAACTATACACATCACAGTAAATCGAGGTGCTGCAAAAGAAGGGGGTCGAGGACCAAGTATATGGGACACATACACTCACAAGCATCCAGGAAGATGTTCGGACGATGAAGGACCTAGGTTTCGATGCTTACAGACTCTCATTCTCATGGTCCAGACTGTTACCAACAACCATTTTCAAGACTATGCAGAGCTCTGCTTTAAAGAATTCGGTGACAGGGTTAAGCATTGGATCACCTTAAATGAGCCCCATAGCTACAGCCGTAATGGCTATGCAACCGGAAAGTTTGCACCAGGTCGTTGTTCTGCTTGGCAAAAGCGAAATTGCACTGGTGGGGATTCAGGGACCGAGCCATACTTAGTGTCACATTACCAACTTCTAGCTCATGCAGCCGCTGTAAAGTTGTATAGACACAAGTATCAGACAAAACAGAATGGCTTGATAGGGATAACACTAGTGTCACACTGGTTTGTGCCATATTCTGATTCAAAGCAAGACCAAGCTGCAGCATTACGAGCATTGGATTTTATGTTTGGATGCCGTGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004338 GO:0004553 GO:0004565 GO:0006082 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009987 GO:0010033 GO:0015925 GO:0015926 GO:0016143 GO:0016145 GO:0016787 GO:0016798 GO:0019137 GO:0019748 GO:0019757 GO:0019759 GO:0019760 GO:0019762 GO:0033907 GO:0042221 GO:0043436 GO:0044237 GO:0044248 GO:0044273 GO:0044281 GO:0047701 GO:0050896 GO:0071704 GO:0080083 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658

KEGG Pathways

Metabolic & Signaling

ko00380 ko00460 ko00500 ko00940 ko01100 ko01110 map00380 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

756

Amino Acids

84.99

Weight (kDa)

9.17

Isoelectric Point (pI)

40.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	71 - 98	5.6e-07	Glycosyl hydrolase family 1
Glyco_hydro_1	PF00232	99 - 250	8.1e-42	Glycosyl hydrolase family 1
Glyco_hydro_1	PF00232	274 - 327	5.2e-17	Glycosyl hydrolase family 1
Glyco_hydro_1	PF00232	630 - 752	2.2e-27	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005175)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g33570
pyrus_communis	pycom05g09700 pycom05g09740 pycom05g10280 pycom05g10310 pycom05g10340 pycom05g13120 pycom11g01900
rosa_chinensis	RchiOBHm_Chr5g0077931
rosa_laevigata	RLG00000035190 RLG00000036728 RLG00000036733
rosa_multiflora	Rmu_co7982122.1_g000001 Rmu_sc0000177.1_g000015 Rmu_sc0001711.1_g000004 Rmu_sc0001711.1_g000025 Rmu_sc0002475.1_g000022 Rmu_sc0004240.1_g000003 Rmu_sc0006499.1_g000002 Rmu_sc0006568.1_g000005 Rmu_sc0006862.1_g000003 Rmu_sc0011139.1_g000001
rosa_roxburghii	Rroxscaffold_1G00000250 Rroxscaffold_1G00004230 Rroxscaffold_1G00004270 Rroxscaffold_4G00277970
rosa_samantha	Rh2BG409200 Rh2BG603900 Rh5DG509600 Rh6BG197900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	103
AccB1I	GGYRCC	1 cut(s)	1391
AccB7I	CCANNNNNTGG	2 cut(s)	276, 1465
AccII	CGCG	2 cut(s)	1352, 1421
AccIII	TCCGGA	1 cut(s)	1429
AciI	CCGC	7 cut(s)	1352, 1355, 1380, 1421, 1564, 1570, 2115
AclWI	GGATC	5 cut(s)	336, 455, 1241, 1440, 1961
AcsI	RAATTY	3 cut(s)	422, 553, 1928
AcuI	CTGAAG	2 cut(s)	122, 1298
AcyI	GRCGYC	1 cut(s)	803
AfaI	GTAC	1 cut(s)	985
AfiI	CCNNNNNNNGG	6 cut(s)	95, 276, 1420, 1465, 1546, 1672
AflII	CTTAAG	1 cut(s)	367
AgeI	ACCGGT	2 cut(s)	545, 1539
AgsI	TTSAA	8 cut(s)	22, 418, 844, 1198, 1295, 1702, 1901, 2211
AhlI	ACTAGT	1 cut(s)	2176
AjiI	CACGTC	1 cut(s)	1652
AjnI	CCWGG	7 cut(s)	216, 292, 464, 822, 927, 1810, 2014
Alw21I	GWGCWC	4 cut(s)	103, 1539, 1615, 1917
Alw44I	GTGCAC	2 cut(s)	1535, 1611
AlwI	GGATC	5 cut(s)	336, 455, 1241, 1440, 1961
AlwNI	CAGNNNCTG	1 cut(s)	2117
Ama87I	CYCGRG	1 cut(s)	1516
Aor13HI	TCCGGA	1 cut(s)	1429
ApaLI	GTGCAC	2 cut(s)	1535, 1611
ApoI	RAATTY	3 cut(s)	422, 553, 1928
ArsI	GACNNNNNNTTYG	2 cut(s)	1827, 1859
AseI	ATTAAT	1 cut(s)	1335
AsiGI	ACCGGT	2 cut(s)	545, 1539
Asp700I	GAANNNNTTC	1 cut(s)	1819
AspA2I	CCTAGG	2 cut(s)	1666, 1838
AspLEI	GCGC	2 cut(s)	1476, 1558
AspS9I	GGNCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
AsuC2I	CCSGG	1 cut(s)	1415
AsuHPI	GGTGA	5 cut(s)	440, 1252, 1496, 1946, 1948
AsuII	TTCGAA	2 cut(s)	152, 758
AsuNHI	GCTAGC	1 cut(s)	1640
AvaI	CYCGRG	1 cut(s)	1516
AvaII	GGWCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
AvrII	CCTAGG	2 cut(s)	1666, 1838
BaeGI	GKGCMC	3 cut(s)	693, 1539, 1615
BaeI	ACNNNNGTAYC	2 cut(s)	2123, 2156
BanI	GGYRCC	1 cut(s)	1391
BanII	GRGCYC	4 cut(s)	465, 1429, 1917, 1971
BarI	GAAGNNNNNNTAC	2 cut(s)	711, 743
Bbv12I	GWGCWC	4 cut(s)	103, 1539, 1615, 1917
BccI	CCATC	9 cut(s)	97, 166, 278, 1128, 1382, 1403, 1481, 1571, 1706
BceAI	ACGGC	3 cut(s)	596, 1968, 2247
BcgI	CGANNNNNNTGC	4 cut(s)	142, 176, 748, 782
BciT130I	CCWGG	7 cut(s)	218, 294, 466, 824, 929, 1812, 2016
BciVI	GTATCC	2 cut(s)	830, 1031
BclI	TGATCA	1 cut(s)	643
BcnI	CCSGG	1 cut(s)	1415
BcuI	ACTAGT	1 cut(s)	2176
BfmI	CTRYAG	2 cut(s)	1978, 2226
BfoI	RGCGCY	1 cut(s)	1477
BfrI	CTTAAG	1 cut(s)	367
BfuAI	ACCTGC	1 cut(s)	103
BfuI	GTATCC	2 cut(s)	830, 1031
BglI	GCCNNNNNGGC	1 cut(s)	1989
BglII	AGATCT	1 cut(s)	8
BlnI	CCTAGG	2 cut(s)	1666, 1838
BmcAI	AGTACT	1 cut(s)	985
Bme1390I	CCNGG	8 cut(s)	218, 294, 466, 824, 929, 1415, 1812, 2016
Bme18I	GGWCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
BmeT110I	CYCGRG	1 cut(s)	1516
BmgBI	CACGTC	1 cut(s)	1652
BmgT120I	GGNCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
BmiI	GGNNCC	3 cut(s)	1393, 1428, 2068
BmrFI	CCNGG	8 cut(s)	218, 294, 466, 824, 929, 1415, 1812, 2016
BmsI	GCATC	9 cut(s)	240, 244, 691, 850, 1162, 1500, 1816, 1838, 2249
BmtI	GCTAGC	1 cut(s)	1644
BpmI	CTGGAG	1 cut(s)	487
Bpu10I	CCTNAGC	1 cut(s)	1077
Bpu14I	TTCGAA	2 cut(s)	152, 758
BpuEI	CTTGAG	1 cut(s)	1283
BpuMI	CCSGG	1 cut(s)	1415
BsaBI	GATNNNNATC	2 cut(s)	13, 1308
BsaHI	GRCGYC	1 cut(s)	803
BsaJI	CCNNGG	4 cut(s)	464, 1201, 1666, 1838
BsaWI	WCCGGW	5 cut(s)	95, 545, 1429, 1539, 2000
BsaXI	ACNNNNNCTCC	2 cut(s)	251, 281
Bsc4I	CCNNNNNNNGG	6 cut(s)	95, 276, 1420, 1465, 1546, 1672
Bse118I	RCCGGY	2 cut(s)	545, 1539
Bse1I	ACTGG	5 cut(s)	147, 449, 686, 2056, 2192
Bse3DI	GCAATG	1 cut(s)	1194
Bse8I	GATNNNNATC	2 cut(s)	13, 1308
BseAI	TCCGGA	1 cut(s)	1429
BseBI	CCWGG	7 cut(s)	218, 294, 466, 824, 929, 1812, 2016
BseDI	CCNNGG	4 cut(s)	464, 1201, 1666, 1838
BseGI	GGATG	8 cut(s)	229, 758, 865, 1139, 1211, 1240, 1807, 2264
BseJI	GATNNNNATC	2 cut(s)	13, 1308
BseLI	CCNNNNNNNGG	6 cut(s)	95, 276, 1420, 1465, 1546, 1672
BseMI	GCAATG	1 cut(s)	1194
BseMII	CTCAG	1 cut(s)	902
BseNI	ACTGG	5 cut(s)	147, 449, 686, 2056, 2192
BseRI	GAGGAG	2 cut(s)	1228, 1566
BseSI	GKGCMC	3 cut(s)	693, 1539, 1615
BsgI	GTGCAG	1 cut(s)	1161
Bsh1236I	CGCG	2 cut(s)	1352, 1421
BshNI	GGYRCC	1 cut(s)	1391
BshTI	ACCGGT	2 cut(s)	545, 1539
BsiHKAI	GWGCWC	4 cut(s)	103, 1539, 1615, 1917
BsiHKCI	CYCGRG	1 cut(s)	1516
BsiSI	CCGG	8 cut(s)	96, 109, 546, 1414, 1430, 1452, 1540, 2001
BslFI	GGGAC	8 cut(s)	141, 208, 511, 814, 1430, 1516, 1802, 2080
BslI	CCNNNNNNNGG	6 cut(s)	95, 276, 1420, 1465, 1546, 1672
BsmFI	GGGAC	8 cut(s)	141, 208, 511, 814, 1430, 1516, 1802, 2080
BsoBI	CYCGRG	1 cut(s)	1516
Bsp119I	TTCGAA	2 cut(s)	152, 758
Bsp1286I	GDGCHC	8 cut(s)	103, 465, 693, 1429, 1539, 1615, 1917, 1971
Bsp13I	TCCGGA	1 cut(s)	1429
BspACI	CCGC	7 cut(s)	1352, 1355, 1380, 1421, 1564, 1570, 2115
BspCNI	CTCAG	1 cut(s)	903
BspEI	TCCGGA	1 cut(s)	1429
BspFNI	CGCG	2 cut(s)	1352, 1421
BspHI	TCATGA	2 cut(s)	234, 988
BspLI	GGNNCC	3 cut(s)	1393, 1428, 2068
BspMAI	CTGCAG	1 cut(s)	2230
BspMI	ACCTGC	1 cut(s)	103
BspOI	GCTAGC	1 cut(s)	1644
BspPI	GGATC	5 cut(s)	336, 455, 1241, 1440, 1961
BspT104I	TTCGAA	2 cut(s)	152, 758
BspT107I	GGYRCC	1 cut(s)	1391
BspTI	CTTAAG	1 cut(s)	367
BsrDI	GCAATG	1 cut(s)	1194
BsrFI	RCCGGY	2 cut(s)	545, 1539
BsrI	ACTGG	5 cut(s)	147, 449, 686, 2056, 2192
BssAI	RCCGGY	2 cut(s)	545, 1539
BssECI	CCNNGG	4 cut(s)	464, 1201, 1666, 1838
BssNI	GRCGYC	1 cut(s)	803
BssT1I	CCWWGG	2 cut(s)	1666, 1838
Bst2UI	CCWGG	7 cut(s)	218, 294, 466, 824, 929, 1812, 2016
Bst4CI	ACNGT	9 cut(s)	359, 675, 791, 894, 1068, 1123, 1510, 1741, 1882
Bst6I	CTCTTC	2 cut(s)	171, 323
BstACI	GRCGYC	1 cut(s)	803
BstAFI	CTTAAG	1 cut(s)	367
BstBI	TTCGAA	2 cut(s)	152, 758
BstC8I	GCNNGC	3 cut(s)	1350, 1598, 1642
BstDEI	CTNAG	3 cut(s)	911, 1077, 2080
BstF5I	GGATG	8 cut(s)	229, 758, 865, 1139, 1211, 1240, 1807, 2264
BstFNI	CGCG	2 cut(s)	1352, 1421
BstH2I	RGCGCY	1 cut(s)	1477
BstHHI	GCGC	2 cut(s)	1476, 1558
BstNI	CCWGG	7 cut(s)	218, 294, 466, 824, 929, 1812, 2016
BstSCI	CCNGG	8 cut(s)	216, 292, 464, 822, 927, 1413, 1810, 2014
BstSFI	CTRYAG	2 cut(s)	1978, 2226
BstSLI	GKGCMC	3 cut(s)	693, 1539, 1615
BstUI	CGCG	2 cut(s)	1352, 1421
BstX2I	RGATCY	2 cut(s)	8, 328
BstXI	CCANNNNNNTGG	2 cut(s)	191, 1785
BstYI	RGATCY	2 cut(s)	8, 328
BsuI	GTATCC	2 cut(s)	830, 1031
BtrI	CACGTC	1 cut(s)	1652
BtsCI	GGATG	8 cut(s)	229, 758, 865, 1139, 1211, 1240, 1807, 2264
BtsIMutI	CAGTG	3 cut(s)	679, 2049, 2185
BveI	ACCTGC	1 cut(s)	103
Cac8I	GCNNGC	3 cut(s)	1350, 1598, 1642
CaiI	CAGNNNCTG	1 cut(s)	2117
CciI	TCATGA	2 cut(s)	234, 988
CfoI	GCGC	2 cut(s)	1476, 1558
Cfr10I	RCCGGY	2 cut(s)	545, 1539
Cfr13I	GGNCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
CseI	GACGC	2 cut(s)	811, 1427
CsiI	ACCWGGT	2 cut(s)	292, 2014
Csp6I	GTAC	1 cut(s)	984
CspAI	ACCGGT	2 cut(s)	545, 1539
CviQI	GTAC	1 cut(s)	984
DdeI	CTNAG	3 cut(s)	911, 1077, 2080
DraI	TTTAAA	1 cut(s)	1924
Eam1104I	CTCTTC	2 cut(s)	171, 323
EarI	CTCTTC	2 cut(s)	171, 323
Ecl136II	GAGCTC	1 cut(s)	1915
Eco130I	CCWWGG	2 cut(s)	1666, 1838
Eco24I	GRGCYC	4 cut(s)	465, 1429, 1917, 1971
Eco47I	GGWCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
Eco53kI	GAGCTC	1 cut(s)	1915
Eco57I	CTGAAG	2 cut(s)	122, 1298
Eco88I	CYCGRG	1 cut(s)	1516
EcoICRI	GAGCTC	1 cut(s)	1915
EcoO109I	RGGNCCY	1 cut(s)	1835
EcoRI	GAATTC	1 cut(s)	1928
EcoRII	CCWGG	7 cut(s)	216, 292, 464, 822, 927, 1810, 2014
EcoT14I	CCWWGG	2 cut(s)	1666, 1838
EcoT38I	GRGCYC	4 cut(s)	465, 1429, 1917, 1971
ErhI	CCWWGG	2 cut(s)	1666, 1838
FaqI	GGGAC	8 cut(s)	141, 208, 511, 814, 1430, 1516, 1802, 2080
FauI	CCCGC	1 cut(s)	1414
FauNDI	CATATG	1 cut(s)	190
FbaI	TGATCA	1 cut(s)	643
FokI	GGATG	7 cut(s)	236, 765, 872, 1146, 1218, 1247, 1794
FriOI	GRGCYC	4 cut(s)	465, 1429, 1917, 1971
GlaI	GCGC	2 cut(s)	1475, 1557
GsuI	CTGGAG	1 cut(s)	487
HaeII	RGCGCY	1 cut(s)	1477
HapII	CCGG	8 cut(s)	96, 109, 546, 1414, 1430, 1452, 1540, 2001
HgaI	GACGC	2 cut(s)	811, 1427
HhaI	GCGC	2 cut(s)	1476, 1558
Hin1I	GRCGYC	1 cut(s)	803
Hin6I	GCGC	2 cut(s)	1474, 1556
HinP1I	GCGC	2 cut(s)	1474, 1556
HindIII	AAGCTT	2 cut(s)	339, 1048
HpaII	CCGG	8 cut(s)	96, 109, 546, 1414, 1430, 1452, 1540, 2001
HphI	GGTGA	5 cut(s)	440, 1252, 1496, 1946, 1948
Hpy166II	GTNNAC	5 cut(s)	385, 925, 1126, 1537, 1613
Hpy188I	TCNGA	9 cut(s)	523, 648, 700, 1153, 1205, 1317, 1825, 2145, 2206
Hpy8I	GTNNAC	5 cut(s)	385, 925, 1126, 1537, 1613
Hpy99I	CGWCG	2 cut(s)	82, 1388
HpyCH4III	ACNGT	9 cut(s)	359, 675, 791, 894, 1068, 1123, 1510, 1741, 1882
HpyCH4IV	ACGT	4 cut(s)	80, 500, 1018, 1651
HpyF3I	CTNAG	3 cut(s)	911, 1077, 2080
HpySE526I	ACGT	4 cut(s)	80, 500, 1018, 1651
Hsp92I	GRCGYC	1 cut(s)	803
HspAI	GCGC	2 cut(s)	1474, 1556
Kpn2I	TCCGGA	1 cut(s)	1429
Ksp22I	TGATCA	1 cut(s)	643
LmnI	GCTCC	4 cut(s)	98, 468, 1432, 1553
LweI	GCATC	9 cut(s)	240, 244, 691, 850, 1162, 1500, 1816, 1838, 2249
MabI	ACCWGGT	2 cut(s)	292, 2014
MaeII	ACGT	4 cut(s)	80, 500, 1018, 1651
MflI	RGATCY	2 cut(s)	8, 328
MhlI	GDGCHC	8 cut(s)	103, 465, 693, 1429, 1539, 1615, 1917, 1971
MlyI	GAGTC	4 cut(s)	1148, 1457, 1528, 1852
MmeI	TCCRAC	1 cut(s)	1176
MroI	TCCGGA	1 cut(s)	1429
MroXI	GAANNNNTTC	1 cut(s)	1819
MseI	TTAA	7 cut(s)	368, 438, 455, 1335, 1923, 1944, 1961
MslI	CAYNNNNRTG	4 cut(s)	514, 1496, 1527, 1868
MspA1I	CMGCKG	1 cut(s)	2117
MspCI	CTTAAG	1 cut(s)	367
MspI	CCGG	8 cut(s)	96, 109, 546, 1414, 1430, 1452, 1540, 2001
MspR9I	CCNGG	8 cut(s)	218, 294, 466, 824, 929, 1415, 1812, 2016
MvaI	CCWGG	7 cut(s)	218, 294, 466, 824, 929, 1812, 2016
MvnI	CGCG	2 cut(s)	1352, 1421
NciI	CCSGG	1 cut(s)	1415
NdeI	CATATG	1 cut(s)	190
NheI	GCTAGC	1 cut(s)	1640
NlaIV	GGNNCC	3 cut(s)	1393, 1428, 2068
NmuCI	GTSAC	7 cut(s)	310, 675, 1019, 1163, 1934, 2085, 2181
NspV	TTCGAA	2 cut(s)	152, 758
PaeR7I	CTCGAG	1 cut(s)	1516
PagI	TCATGA	2 cut(s)	234, 988
PcsI	WCGNNNNNNNCGW	2 cut(s)	609, 1514
PdmI	GAANNNNTTC	1 cut(s)	1819
PfeI	GAWTC	6 cut(s)	264, 870, 936, 1443, 2060, 2207
PflMI	CCANNNNNTGG	2 cut(s)	276, 1465
PfoI	TCCNGGA	4 cut(s)	216, 822, 1413, 1810
PinAI	ACCGGT	2 cut(s)	545, 1539
PleI	GAGTC	4 cut(s)	1148, 1456, 1527, 1852
PpsI	GAGTC	4 cut(s)	1148, 1456, 1527, 1852
PpuMI	RGGWCCY	1 cut(s)	1835
PshBI	ATTAAT	1 cut(s)	1335
Psp124BI	GAGCTC	1 cut(s)	1917
Psp5II	RGGWCCY	1 cut(s)	1835
Psp6I	CCWGG	7 cut(s)	216, 292, 464, 822, 927, 1810, 2014
PspGI	CCWGG	7 cut(s)	216, 292, 464, 822, 927, 1810, 2014
PspN4I	GGNNCC	3 cut(s)	1393, 1428, 2068
PspPI	GGNCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
PspPPI	RGGWCCY	1 cut(s)	1835
PspXI	VCTCGAGB	1 cut(s)	1516
PstI	CTGCAG	1 cut(s)	2230
PstNI	CAGNNNCTG	1 cut(s)	2117
PsuI	RGATCY	2 cut(s)	8, 328
RsaI	GTAC	1 cut(s)	985
RsaNI	GTAC	1 cut(s)	984
RseI	CAYNNNNRTG	4 cut(s)	514, 1496, 1527, 1868
SacI	GAGCTC	1 cut(s)	1917
SaqAI	TTAA	7 cut(s)	368, 438, 455, 1335, 1923, 1944, 1961
Sau96I	GGNCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
ScaI	AGTACT	1 cut(s)	985
SchI	GAGTC	4 cut(s)	1148, 1457, 1528, 1852
ScrFI	CCNGG	8 cut(s)	218, 294, 466, 824, 929, 1415, 1812, 2016
SduI	GDGCHC	8 cut(s)	103, 465, 693, 1429, 1539, 1615, 1917, 1971
SexAI	ACCWGGT	2 cut(s)	292, 2014
SfaNI	GCATC	9 cut(s)	240, 244, 691, 850, 1162, 1500, 1816, 1838, 2249
SfcI	CTRYAG	2 cut(s)	1978, 2226
Sfr274I	CTCGAG	1 cut(s)	1516
SfuI	TTCGAA	2 cut(s)	152, 758
SgrAI	CRCCGGYG	2 cut(s)	545, 1539
SinI	GGWCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
SlaI	CTCGAG	1 cut(s)	1516
SmiMI	CAYNNNNRTG	4 cut(s)	514, 1496, 1527, 1868
SmlI	CTYRAG	3 cut(s)	367, 1262, 1516
SmoI	CTYRAG	3 cut(s)	367, 1262, 1516
SpeI	ACTAGT	1 cut(s)	2176
SsiI	CCGC	7 cut(s)	1352, 1355, 1380, 1421, 1564, 1570, 2115
SspI	AATATT	2 cut(s)	944, 1298
SstI	GAGCTC	1 cut(s)	1917
StyD4I	CCNGG	8 cut(s)	216, 292, 464, 822, 927, 1413, 1810, 2014
StyI	CCWWGG	2 cut(s)	1666, 1838
TaaI	ACNGT	9 cut(s)	359, 675, 791, 894, 1068, 1123, 1510, 1741, 1882
TaiI	ACGT	4 cut(s)	83, 503, 1021, 1654
TaqII	GACCGA	1 cut(s)	2084
TatI	WGTACW	1 cut(s)	983
TauI	GCSGC	3 cut(s)	1355, 1383, 2117
TfiI	GAWTC	6 cut(s)	264, 870, 936, 1443, 2060, 2207
Tru1I	TTAA	7 cut(s)	368, 438, 455, 1335, 1923, 1944, 1961
Tru9I	TTAA	7 cut(s)	368, 438, 455, 1335, 1923, 1944, 1961
TscAI	CASTG	3 cut(s)	686, 2056, 2192
TseFI	GTSAC	7 cut(s)	310, 675, 1019, 1163, 1934, 2085, 2181
Tsp45I	GTSAC	7 cut(s)	310, 675, 1019, 1163, 1934, 2085, 2181
TspDTI	ATGAA	8 cut(s)	108, 251, 366, 729, 1020, 1313, 1579, 1845
TspRI	CASTG	3 cut(s)	686, 2056, 2192
Van91I	CCANNNNNTGG	2 cut(s)	276, 1465
Vha464I	CTTAAG	1 cut(s)	367
VneI	GTGCAC	2 cut(s)	1535, 1611
VpaK11BI	GGWCC	6 cut(s)	181, 787, 1775, 1835, 1873, 2067
VspI	ATTAAT	1 cut(s)	1335
XapI	RAATTY	3 cut(s)	422, 553, 1928
XbaI	TCTAGA	2 cut(s)	281, 887
XcmI	CCANNNNNNNNNTGG	1 cut(s)	224
XhoI	CTCGAG	1 cut(s)	1516
XmaJI	CCTAGG	2 cut(s)	1666, 1838
XmnI	GAANNNNTTC	1 cut(s)	1819
ZrmI	AGTACT	1 cut(s)	985

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.