Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0002974

Physical Location & Seq

Genomic Coordinates Forward (+)

14143666 .. 14146166

Gene Structure 2501 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1296 bp

ATGAGGTATGAAAAGTTGTTCTCCAACAAGCAGCCCCGCCTCTCTCTTTCCCCCCTTCTTTCCTCTTCCTCTGGTTCGCCGCCTCACCTTCTCTTTTGCTGCTGCGTTTCTTCTTCTTCCCTCTTCAGCTCTTCAATTCCAAATCCCACAGCAGTCTTGGAGTTTGAGGCCTTTTCTCTGTTGGGTTTTCTTCCAGTAGCTCAAAGCTCCAAAAGTGGTGGGTTCACCATGAACAACCTCTCCGCTTTCAAAGCCCTGGACTCCAGTCCACTCTCCAGCCCTCGCTCCTTTGGGCCCCACAACCACATACCTATTCTCCTCCAGCGCCCGAGGAATGCTGTCTTATGCTCAGTGAAACAATCAATTGCCACCCCTTCACCTCTAAGTGTTGATAGTTCCCCTAACCAGGGGCTTAATCGAATTGAATCACTAACCCAGGTTTCGGGGGTGTTGGGCTGCCAATGGGGTGATGAAGGTAAAGGAAAACTTGTTGACATCTTGGCCCAGCACTTTGACATCGTTGCTCGTTGTCAGGGTGGAGCTAATGCTGGACACACCATTTACAATGCAGAGGGAAAGAAGTTTGCACTTCACCTTGTTCCGTCGGGTATTCTTAATGAAGAAACTCTTTGTGTTATTGGGAATGGAGTTGTGGTGCATCTTCCAGGGCTCTTTAAAGAGATTGATGGCCTTGAATCTAATGGAGTCTCCTGCAAGGGAAGGATATTGATCTCTGATCGTGCTCACCTGTTATTTGATTTCCACCAAGTAGTGGATGGACTTAGAGAATCTGAGCTTGCTAAATCATTCATTGGCACTACCAAGAGGGGCATTGGACCATGTTACTCTAACAAGGTTAACCGAAATGGTATTCGAGTGAGTGACTTGAGGTACATGGATACTTTTCCTCAGAAGCTAGATCTTCTATTATCAGATGCTGCATCAAGATTCCAAGGTTTTGAGTATGGCCCCCATGTGCTCAAAGAAGAAGTCGAAAAATACAAGAGATTTGCTGAGAGATTGGAACCCTTCATTGCGGATACTGTGCTTGTCGTGAATGAAGCCATCTCTGAGCAGAAAAAGATTTTGGTCGAAGGTGGTCAGGCAACAATGTTGGATATTGACTTTGGAACTTACCCCTTTGTAACATCCTCTAGCCCATCAGCTGGTGGGATTTGCACTGGTCTCGGAATTGCTCCCAGAGTTTTAGGTCGATCTGGTTGGAGTGGTGAAAGCGTACACTACAAGAGTTGGTTCTGGTCCCTTTCCCACAGAAATCTTGGGTCAAGGGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004019 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006163 GO:0006164 GO:0006167 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010035 GO:0010038 GO:0016874 GO:0016879 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0034641 GO:0034654 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044208 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046033 GO:0046040 GO:0046390 GO:0046483 GO:0046686 GO:0048046 GO:0050896 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00230 ko00250 ko01100 map00230 map00250 map01100

Pfam Domains

Protein Families

Adenylsucc_synt

Protein Analysis

432

Amino Acids

46.69

Weight (kDa)

8.46

Isoelectric Point (pI)

45.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Adenylsucc_synt	PF00709	150 - 403	2.4e-102	Adenylosuccinate synthetase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002974)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57610
fragaria_vesca	FvH4_2g02690 FvH4_7g07940
malus_domestica	MD00G1080700.v1.1 MD05G1111200.v1.1 MD15G1386600.v1.1
prunus_persica	Prupe.5G015200_v2.0.a1 Prupe.5G015200_v2.0.a1 Prupe.5G015200_v2.0.a1 Prupe.5G015200_v2.0.a1 Prupe.8G156300_v2.0.a1 Prupe.8G156300_v2.0.a1 Prupe.8G156300_v2.0.a1
pyrus_communis	pycom05g10700 pycom10g09910 pycom15g34650
rosa_chinensis	RchiOBHm_Chr6g0247091 RchiOBHm_Chr6g0247101 RchiOBHm_Chr7g0177111 RchiOBHm_Chr7g0177151
rosa_laevigata	RLG00000015220 RLG00000030365
rosa_multiflora	Rmu_sc0005310.1_g000020 Rmu_sc0010391.1_g000001 Rmu_sc0012524.1_g000001
rosa_roxburghii	Rroxscaffold_3G00276060 Rroxscaffold_7G00214350
rosa_rugosa	Rorug05G0522100 Rorug05G0522200 Rorug05G0522300
rosa_samantha	Rh6AG037300 Rh6BG032200 Rh6CG033300 Rh6DG031000 Rh7AG003500 Rh7CG003200
rosa_wichuraiana	Rw6G003200 Rw7G000290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	772, 1166
AciI	CCGC	4 cut(s)	37, 80, 243, 1037
AcuI	CTGAAG	1 cut(s)	109
AfaI	GTAC	2 cut(s)	893, 1239
AfiI	CCNNNNNNNGG	5 cut(s)	406, 407, 442, 772, 1166
AgsI	TTSAA	4 cut(s)	135, 250, 425, 695
AjnI	CCWGG	4 cut(s)	255, 405, 435, 664
AjuI	GAANNNNNNNTTGG	2 cut(s)	1070, 1102
AloI	GAACNNNNNNTCC	2 cut(s)	224, 256
AluBI	AGCT	7 cut(s)	129, 200, 207, 542, 796, 916, 1166
AluI	AGCT	7 cut(s)	129, 200, 207, 542, 796, 916, 1166
Alw21I	GWGCWC	2 cut(s)	745, 981
Alw26I	GTCTC	2 cut(s)	712, 1190
AlwNI	CAGNNNCTG	1 cut(s)	938
Ama87I	CYCGRG	1 cut(s)	328
AoxI	GGCC	5 cut(s)	168, 293, 501, 688, 967
ApaI	GGGCCC	1 cut(s)	297
ApeKI	GCWGC	5 cut(s)	31, 99, 102, 456, 938
ArsI	GACNNNNNNTTYG	2 cut(s)	975, 1007
AspLEI	GCGC	1 cut(s)	327
AspS9I	GGNCC	6 cut(s)	293, 294, 502, 836, 968, 1260
AsuHPI	GGTGA	7 cut(s)	77, 217, 369, 479, 584, 737, 1241
AvaI	CYCGRG	1 cut(s)	328
AvaII	GGWCC	2 cut(s)	836, 1260
BaeGI	GKGCMC	1 cut(s)	297
BanII	GRGCYC	2 cut(s)	297, 672
Bbv12I	GWGCWC	2 cut(s)	745, 981
BbvI	GCAGC	5 cut(s)	43, 86, 89, 443, 925
BccI	CCATC	4 cut(s)	680, 770, 1073, 1168
BcgI	CGANNNNNNTGC	2 cut(s)	1168, 1202
BciT130I	CCWGG	4 cut(s)	257, 407, 437, 666
BciVI	GTATCC	2 cut(s)	892, 1033
BcoDI	GTCTC	2 cut(s)	712, 1190
BfaI	CTAG	2 cut(s)	917, 1155
BfoI	RGCGCY	1 cut(s)	328
BfuI	GTATCC	2 cut(s)	892, 1033
BglII	AGATCT	1 cut(s)	919
BisI	GCNGC	6 cut(s)	32, 80, 100, 103, 457, 939
BlsI	GCNGC	6 cut(s)	33, 81, 101, 104, 458, 940
Bme1390I	CCNGG	4 cut(s)	257, 407, 437, 666
Bme18I	GGWCC	2 cut(s)	836, 1260
BmeT110I	CYCGRG	1 cut(s)	328
BmgT120I	GGNCC	6 cut(s)	293, 294, 502, 836, 968, 1260
BmiI	GGNNCC	5 cut(s)	295, 296, 970, 1026, 1262
BmrFI	CCNGG	4 cut(s)	257, 407, 437, 666
BmsI	GCATC	3 cut(s)	667, 925, 950
BoxI	GACNNNNGTC	1 cut(s)	264
BpmI	CTGGAG	3 cut(s)	247, 259, 305
BpuEI	CTTGAG	1 cut(s)	907
BsaBI	GATNNNNATC	1 cut(s)	728
BsaI	GGTCTC	1 cut(s)	1190
BsaJI	CCNNGG	6 cut(s)	255, 329, 406, 435, 665, 952
BsaXI	ACNNNNNCTCC	6 cut(s)	224, 254, 300, 330, 639, 669
Bsc4I	CCNNNNNNNGG	5 cut(s)	406, 407, 442, 772, 1166
Bse1I	ACTGG	3 cut(s)	194, 264, 1186
Bse3DI	GCAATG	1 cut(s)	1032
Bse8I	GATNNNNATC	1 cut(s)	728
BseBI	CCWGG	4 cut(s)	257, 407, 437, 666
BseDI	CCNNGG	6 cut(s)	255, 329, 406, 435, 665, 952
BseGI	GGATG	2 cut(s)	781, 1148
BseJI	GATNNNNATC	1 cut(s)	728
BseLI	CCNNNNNNNGG	5 cut(s)	406, 407, 442, 772, 1166
BseMI	GCAATG	1 cut(s)	1032
BseMII	CTCAG	5 cut(s)	363, 783, 923, 1005, 1062
BseNI	ACTGG	3 cut(s)	194, 264, 1186
BseRI	GAGGAG	1 cut(s)	308
BseSI	GKGCMC	1 cut(s)	297
BseXI	GCAGC	5 cut(s)	43, 86, 89, 443, 925
BseYI	CCCAGC	1 cut(s)	504
BshFI	GGCC	5 cut(s)	170, 295, 503, 690, 969
BsiHKAI	GWGCWC	2 cut(s)	745, 981
BsiHKCI	CYCGRG	1 cut(s)	328
BslFI	GGGAC	1 cut(s)	1246
BslI	CCNNNNNNNGG	5 cut(s)	406, 407, 442, 772, 1166
BsmAI	GTCTC	2 cut(s)	712, 1190
BsmFI	GGGAC	1 cut(s)	1246
BsmI	GAATGC	1 cut(s)	340
BsnI	GGCC	5 cut(s)	170, 295, 503, 690, 969
Bso31I	GGTCTC	1 cut(s)	1190
BsoBI	CYCGRG	1 cut(s)	328
Bsp120I	GGGCCC	1 cut(s)	293
Bsp1286I	GDGCHC	4 cut(s)	297, 672, 745, 981
Bsp143I	GATC	4 cut(s)	729, 736, 919, 1214
BspACI	CCGC	4 cut(s)	37, 80, 243, 1037
BspANI	GGCC	5 cut(s)	170, 295, 503, 690, 969
BspCNI	CTCAG	5 cut(s)	362, 784, 922, 1006, 1063
BspLI	GGNNCC	5 cut(s)	295, 296, 970, 1026, 1262
BspQI	GCTCTTC	1 cut(s)	136
BspTNI	GGTCTC	1 cut(s)	1190
BsrDI	GCAATG	1 cut(s)	1032
BsrI	ACTGG	3 cut(s)	194, 264, 1186
BssECI	CCNNGG	6 cut(s)	255, 329, 406, 435, 665, 952
BssMI	GATC	4 cut(s)	729, 736, 919, 1214
BssT1I	CCWWGG	1 cut(s)	952
Bst2UI	CCWGG	4 cut(s)	257, 407, 437, 666
Bst4CI	ACNGT	1 cut(s)	1045
Bst6I	CTCTTC	3 cut(s)	70, 128, 136
BstC8I	GCNNGC	1 cut(s)	798
BstDEI	CTNAG	7 cut(s)	349, 383, 782, 792, 909, 1014, 1071
BstF5I	GGATG	2 cut(s)	781, 1148
BstH2I	RGCGCY	1 cut(s)	328
BstHHI	GCGC	1 cut(s)	327
BstKTI	GATC	4 cut(s)	732, 739, 922, 1217
BstMAI	GTCTC	2 cut(s)	712, 1190
BstMBI	GATC	4 cut(s)	729, 736, 919, 1214
BstMWI	GCNNNNNNNGC	1 cut(s)	251
BstNI	CCWGG	4 cut(s)	257, 407, 437, 666
BstPAI	GACNNNNGTC	1 cut(s)	264
BstSCI	CCNGG	4 cut(s)	255, 405, 435, 664
BstSLI	GKGCMC	1 cut(s)	297
BstV1I	GCAGC	5 cut(s)	43, 86, 89, 443, 925
BstX2I	RGATCY	1 cut(s)	919
BstYI	RGATCY	1 cut(s)	919
BsuI	GTATCC	2 cut(s)	892, 1033
BsuRI	GGCC	5 cut(s)	170, 295, 503, 690, 969
BtsCI	GGATG	2 cut(s)	781, 1148
BtsIMutI	CAGTG	2 cut(s)	357, 1179
Cac8I	GCNNGC	1 cut(s)	798
CaiI	CAGNNNCTG	1 cut(s)	938
CfoI	GCGC	1 cut(s)	327
Cfr13I	GGNCC	6 cut(s)	293, 294, 502, 836, 968, 1260
Csp6I	GTAC	2 cut(s)	892, 1238
CspCI	CAANNNNNGTGG	2 cut(s)	199, 234
CviAII	CATG	4 cut(s)	229, 840, 895, 974
CviQI	GTAC	2 cut(s)	892, 1238
DdeI	CTNAG	7 cut(s)	349, 383, 782, 792, 909, 1014, 1071
DpnI	GATC	4 cut(s)	731, 738, 921, 1216
DpnII	GATC	4 cut(s)	729, 736, 919, 1214
DraI	TTTAAA	1 cut(s)	676
Eam1104I	CTCTTC	3 cut(s)	70, 128, 136
EarI	CTCTTC	3 cut(s)	70, 128, 136
Eco130I	CCWWGG	1 cut(s)	952
Eco147I	AGGCCT	1 cut(s)	170
Eco24I	GRGCYC	2 cut(s)	297, 672
Eco31I	GGTCTC	1 cut(s)	1190
Eco47I	GGWCC	2 cut(s)	836, 1260
Eco57I	CTGAAG	1 cut(s)	109
Eco88I	CYCGRG	1 cut(s)	328
EcoO109I	RGGNCCY	1 cut(s)	294
EcoRII	CCWGG	4 cut(s)	255, 405, 435, 664
EcoT14I	CCWWGG	1 cut(s)	952
EcoT38I	GRGCYC	2 cut(s)	297, 672
ErhI	CCWWGG	1 cut(s)	952
FaeI	CATG	4 cut(s)	232, 843, 898, 977
FaiI	YATR	8 cut(s)	9, 230, 308, 346, 841, 896, 966, 975
FalI	AAGNNNNNCTT	4 cut(s)	471, 503, 612, 644
FaqI	GGGAC	1 cut(s)	1246
FatI	CATG	4 cut(s)	228, 839, 894, 973
FauI	CCCGC	1 cut(s)	44
Fnu4HI	GCNGC	6 cut(s)	32, 80, 100, 103, 457, 939
FokI	GGATG	2 cut(s)	788, 1135
FriOI	GRGCYC	2 cut(s)	297, 672
Fsp4HI	GCNGC	6 cut(s)	32, 80, 100, 103, 457, 939
FspBI	CTAG	2 cut(s)	917, 1155
GlaI	GCGC	1 cut(s)	326
GluI	GCNGC	6 cut(s)	32, 80, 100, 103, 457, 939
GsaI	CCCAGC	1 cut(s)	508
GsuI	CTGGAG	3 cut(s)	247, 259, 305
HaeII	RGCGCY	1 cut(s)	328
HaeIII	GGCC	5 cut(s)	170, 295, 503, 690, 969
HhaI	GCGC	1 cut(s)	327
Hin1II	CATG	4 cut(s)	232, 843, 898, 977
Hin6I	GCGC	1 cut(s)	325
HinP1I	GCGC	1 cut(s)	325
HincII	GTYRAC	2 cut(s)	493, 859
HindII	GTYRAC	2 cut(s)	493, 859
HinfI	GANTC	6 cut(s)	260, 425, 695, 705, 788, 948
HpaI	GTTAAC	1 cut(s)	859
HphI	GGTGA	7 cut(s)	77, 217, 369, 479, 584, 737, 1241
Hpy166II	GTNNAC	5 cut(s)	225, 269, 493, 859, 1240
Hpy188I	TCNGA	6 cut(s)	736, 793, 912, 934, 1072, 1190
Hpy188III	TCNNGA	2 cut(s)	945, 1054
Hpy8I	GTNNAC	5 cut(s)	225, 269, 493, 859, 1240
Hpy99I	CGWCG	1 cut(s)	607
HpyAV	CCTTC	7 cut(s)	65, 98, 384, 467, 714, 1039, 1088
HpyCH4III	ACNGT	1 cut(s)	1045
HpyCH4V	TGCA	6 cut(s)	569, 587, 658, 714, 941, 1179
HpyF10VI	GCNNNNNNNGC	1 cut(s)	251
HpyF3I	CTNAG	7 cut(s)	349, 383, 782, 792, 909, 1014, 1071
Hsp92II	CATG	4 cut(s)	232, 843, 898, 977
HspAI	GCGC	1 cut(s)	325
KspAI	GTTAAC	1 cut(s)	859
Kzo9I	GATC	4 cut(s)	729, 736, 919, 1214
LguI	GCTCTTC	1 cut(s)	136
LmnI	GCTCC	4 cut(s)	212, 290, 539, 1201
Lsp1109I	GCAGC	5 cut(s)	43, 86, 89, 443, 925
LweI	GCATC	3 cut(s)	667, 925, 950
MaeI	CTAG	2 cut(s)	917, 1155
MaeIII	GTNAC	3 cut(s)	842, 881, 1144
MalI	GATC	4 cut(s)	731, 738, 921, 1216
MboI	GATC	4 cut(s)	729, 736, 919, 1214
MfeI	CAATTG	1 cut(s)	363
MflI	RGATCY	1 cut(s)	919
MhlI	GDGCHC	4 cut(s)	297, 672, 745, 981
MluCI	AATT	4 cut(s)	135, 363, 420, 1191
MlyI	GAGTC	2 cut(s)	254, 714
MmeI	TCCRAC	3 cut(s)	48, 1095, 1202
MseI	TTAA	4 cut(s)	414, 615, 675, 858
MspA1I	CMGCKG	1 cut(s)	1166
MspR9I	CCNGG	4 cut(s)	257, 407, 437, 666
MunI	CAATTG	1 cut(s)	363
Mva1269I	GAATGC	1 cut(s)	340
MvaI	CCWGG	4 cut(s)	257, 407, 437, 666
MwoI	GCNNNNNNNGC	1 cut(s)	251
NdeII	GATC	4 cut(s)	729, 736, 919, 1214
NlaIII	CATG	4 cut(s)	232, 843, 898, 977
NlaIV	GGNNCC	5 cut(s)	295, 296, 970, 1026, 1262
NmuCI	GTSAC	1 cut(s)	881
PceI	AGGCCT	1 cut(s)	170
PciSI	GCTCTTC	1 cut(s)	136
PctI	GAATGC	1 cut(s)	340
PfeI	GAWTC	4 cut(s)	425, 695, 788, 948
PflMI	CCANNNNNTGG	2 cut(s)	772, 1166
PkrI	GCNGC	6 cut(s)	33, 81, 101, 104, 458, 940
PleI	GAGTC	2 cut(s)	254, 713
PpsI	GAGTC	2 cut(s)	254, 713
PshAI	GACNNNNGTC	1 cut(s)	264
Psp6I	CCWGG	4 cut(s)	255, 405, 435, 664
PspFI	CCCAGC	1 cut(s)	504
PspGI	CCWGG	4 cut(s)	255, 405, 435, 664
PspN4I	GGNNCC	5 cut(s)	295, 296, 970, 1026, 1262
PspOMI	GGGCCC	1 cut(s)	293
PspPI	GGNCC	6 cut(s)	293, 294, 502, 836, 968, 1260
PstNI	CAGNNNCTG	1 cut(s)	938
PsuI	RGATCY	1 cut(s)	919
PvuII	CAGCTG	1 cut(s)	1166
RsaI	GTAC	2 cut(s)	893, 1239
RsaNI	GTAC	2 cut(s)	892, 1238
SapI	GCTCTTC	1 cut(s)	136
SaqAI	TTAA	4 cut(s)	414, 615, 675, 858
SatI	GCNGC	6 cut(s)	32, 80, 100, 103, 457, 939
Sau3AI	GATC	4 cut(s)	729, 736, 919, 1214
Sau96I	GGNCC	6 cut(s)	293, 294, 502, 836, 968, 1260
SchI	GAGTC	2 cut(s)	254, 714
ScrFI	CCNGG	4 cut(s)	257, 407, 437, 666
SduI	GDGCHC	4 cut(s)	297, 672, 745, 981
SfaNI	GCATC	3 cut(s)	667, 925, 950
SinI	GGWCC	2 cut(s)	836, 1260
SmlI	CTYRAG	1 cut(s)	886
SmoI	CTYRAG	1 cut(s)	886
Sse9I	AATT	4 cut(s)	135, 363, 420, 1191
SseBI	AGGCCT	1 cut(s)	170
SsiI	CCGC	4 cut(s)	37, 80, 243, 1037
SspMI	CTAG	2 cut(s)	917, 1155
StuI	AGGCCT	1 cut(s)	170
StyD4I	CCNGG	4 cut(s)	255, 405, 435, 664
StyI	CCWWGG	1 cut(s)	952
TaaI	ACNGT	1 cut(s)	1045
TaqI	TCGA	5 cut(s)	418, 874, 993, 1092, 1213
TasI	AATT	4 cut(s)	135, 363, 420, 1191
TauI	GCSGC	1 cut(s)	82
TfiI	GAWTC	4 cut(s)	425, 695, 788, 948
Tru1I	TTAA	4 cut(s)	414, 615, 675, 858
Tru9I	TTAA	4 cut(s)	414, 615, 675, 858
TscAI	CASTG	2 cut(s)	357, 1186
TseFI	GTSAC	1 cut(s)	881
TseI	GCWGC	5 cut(s)	31, 99, 102, 456, 938
Tsp45I	GTSAC	1 cut(s)	881
TspDTI	ATGAA	7 cut(s)	24, 245, 486, 633, 799, 1021, 1074
TspGWI	ACGGA	1 cut(s)	591
TspRI	CASTG	2 cut(s)	357, 1186
Van91I	CCANNNNNTGG	2 cut(s)	772, 1166
VpaK11BI	GGWCC	2 cut(s)	836, 1260
XcmI	CCANNNNNNNNNTGG	3 cut(s)	154, 773, 1277
XspI	CTAG	2 cut(s)	917, 1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.