pycom05g32330

Transcription initiation factor IIF subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001724
Physical Location & Seq
Reverse (-)
31771735 .. 31775499
3765 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1527 bp
ATGGCTCTCAACCGCGCCAAGTGCAACGAGTGCGGCGCCGTCGTCACTCGCTTAATTCGAGAATATAATGTTCGAGCGAGTACTGTCAATGACAAGAACTACTTCATTGGGAGATTTGTGACGGGGTTGCCGAGTTTTTCGAAGACGAAGAATTCTGAAAATAAATGGTCTCTCCACAAGGATGGACTACAAGGACGTCAACTTAGTGATGCTATGCGGGAGAAGTACAAGAATAAACCCTGGGTTTTGGAGGACGAAAGTGGCCGGTCTCAGTACCAGGGTCATCTTGAAGGTGCACAGACTGCAACCTACTACTTGCTAATAAAGCATCCCTCTGGAAAGGAGTTTTCTGCCATTCCTGCTGGTTCTTGGTTCAATTTCAACAAGGTTGCACAGTACAAACAACTTACATTGGAGGAAGCCGAAGAGAAGATTAGTAATAGGAAAAAGACTGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAATAATGGAGCTGCTTTATTTGGTGAAGTGGAGAGGTTTGACAACGATAATGGTGTGGCTGGTGGGAAGGGACGAAAAAAGACAGCTGCTGGTGGTGATGATGAAGAAGGAAATGGTTCAGATAGAGGAGATGAGGATGATGAAGAAGAGTTGGAAAGGAAGAAAAGATTGAACAAAAGTGGTGGTGACGGTGATGGTGATGATGATGATGAAGGCGCAAGGGGAGGTGATGCGGACATGGATGACGATGATATCGAGAAGGGTGATGACTGGGAGCACGAAGAAATTTTCACTGATGATGATGAAGCTGTTGGCAACAATCCTGAGGAAAGGGAAGATTTGGAACCAGAGATTCCAGCTCCTCCAGAAATTAAGCAGGATGATGAGGACGAGGATGAAGATGACAAAGAGGGTGGGCTGAGCAAATCAGGAAAAGAGTTGAAGAAGCTACTTGGGCGAAGTGGTGGGCTGAATGATTCAGATGTGGAGGATGACGACGATGATGATGATGATATGGATGATGATATTGGCTTTCCTTCAGAGCTGGCTCCAAAGCAGAAGGATGCACCCAAAGAGGAACCATCTGACAACAGTCCCTCAAAACCAACACCTTCTGCGTCCAGTCGAGGAACTACATCAACCTCCAAGTCCTCAAAGGGAAAGAGAAAATCAAGTGGCGATGATGCTAAGGCATCTAACAGTGCACCTCCCAAGAAGGTCAAGACTGAAAATGAACAAAAATCCATCAAAGAGGAGCCTGTGTCTGTACCTGCTTCTAAAAGTAGTGCGCCTCCAAAAGGTACTCCGCCGGTGAAAACAGAGCCATCATCATCTGGTGGACGTGTCACCGAGGAAGAAATCAGGGCCGTGTTGATGCAGAGAACACCACTTACCACGAATGATCTTGTTTCTAGATTTAAGGGAAGACTCAAATCGTCTGAGGAAAAGGCTGCTTTTTCGAGTATTCTAAGGAGAATTTCTAAGATAAAGAAGGGCAACAACGGAACCAGCAGCTTTATAGTACTGAAAGAACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008022
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
TFIIF_alpha

Protein Analysis

509

Amino Acids

55.79

Weight (kDa)

4.84

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIF_alpha PF05793 10 - 505 3.9e-138 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 199
Acc36I ACCTGC 1 cut(s) 1267
AccB1I GGYRCC 1 cut(s) 35
AccII CGCG 1 cut(s) 15
AciI CCGC 5 cut(s) 13, 33, 217, 722, 1295
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 3 cut(s) 151, 774, 1464
AcuI CTGAAG 1 cut(s) 1011
AcyI GRCGYC 2 cut(s) 36, 196
AfaI GTAC 7 cut(s) 82, 227, 275, 398, 1257, 1291, 1512
AfiI CCNNNNNNNGG 1 cut(s) 1286
AflIII ACRYGT 1 cut(s) 1330
AgsI TTSAA 5 cut(s) 290, 376, 382, 661, 931
AjiI CACGTC 1 cut(s) 1331
AjnI CCWGG 2 cut(s) 239, 276
AluBI AGCT 8 cut(s) 485, 500, 575, 797, 848, 937, 1033, 1503
AluI AGCT 8 cut(s) 485, 500, 575, 797, 848, 937, 1033, 1503
Alw21I GWGCWC 3 cut(s) 298, 768, 1195
Alw26I GTCTC 2 cut(s) 174, 273
Alw44I GTGCAC 2 cut(s) 294, 1191
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 2 cut(s) 262, 1353
ApaLI GTGCAC 2 cut(s) 294, 1191
ApeKI GCWGC 5 cut(s) 485, 500, 575, 1439, 1500
ApoI RAATTY 3 cut(s) 151, 774, 1464
Asp700I GAANNNNTTC 2 cut(s) 101, 604
AspLEI GCGC 4 cut(s) 17, 38, 707, 1279
AspS9I GGNCC 1 cut(s) 1353
AsuHPI GGTGA 9 cut(s) 524, 596, 686, 692, 698, 728, 764, 1312, 1327
AsuII TTCGAA 1 cut(s) 140
AxyI CCTNAGG 1 cut(s) 813
BaeGI GKGCMC 2 cut(s) 298, 1195
BanI GGYRCC 1 cut(s) 35
BbsI GAAGAC 2 cut(s) 149, 1420
Bbv12I GWGCWC 3 cut(s) 298, 768, 1195
BbvI GCAGC 5 cut(s) 472, 487, 562, 1426, 1512
BccI CCATC 7 cut(s) 176, 452, 465, 677, 1078, 1241, 1321
BceAI ACGGC 2 cut(s) 23, 1340
BciT130I CCWGG 2 cut(s) 241, 278
BcoDI GTCTC 2 cut(s) 174, 273
BfaI CTAG 1 cut(s) 1401
BfmI CTRYAG 1 cut(s) 453
BfoI RGCGCY 1 cut(s) 39
BfuAI ACCTGC 1 cut(s) 1267
BisI GCNGC 6 cut(s) 34, 486, 501, 576, 1440, 1501
BlpI GCTNAGC 1 cut(s) 908
BlsI GCNGC 6 cut(s) 35, 487, 502, 577, 1441, 1502
BmcAI AGTACT 2 cut(s) 82, 1512
Bme1390I CCNGG 2 cut(s) 241, 278
BmgBI CACGTC 1 cut(s) 1331
BmgT120I GGNCC 1 cut(s) 1353
BmiI GGNNCC 6 cut(s) 37, 834, 1038, 1068, 1245, 1495
BmrFI CCNGG 2 cut(s) 241, 278
BmrI ACTGGG 1 cut(s) 769
BmsI GCATC 7 cut(s) 199, 337, 709, 1042, 1162, 1190, 1353
BmuI ACTGGG 1 cut(s) 769
BoxI GACNNNNGTC 1 cut(s) 1080
BpiI GAAGAC 2 cut(s) 149, 1420
BpmI CTGGAG 1 cut(s) 837
Bpu10I CCTNAGC 1 cut(s) 1176
Bpu1102I GCTNAGC 1 cut(s) 908
Bpu14I TTCGAA 1 cut(s) 140
BsaHI GRCGYC 2 cut(s) 36, 196
BsaI GGTCTC 2 cut(s) 174, 273
BsaJI CCNNGG 4 cut(s) 239, 240, 277, 1338
BsaXI ACNNNNNCTCC 2 cut(s) 1235, 1265
Bsc4I CCNNNNNNNGG 1 cut(s) 1286
Bse118I RCCGGY 2 cut(s) 264, 1297
Bse1I ACTGG 2 cut(s) 764, 1110
Bse21I CCTNAGG 1 cut(s) 813
BseBI CCWGG 2 cut(s) 241, 278
BseDI CCNNGG 4 cut(s) 239, 240, 277, 1338
BseLI CCNNNNNNNGG 1 cut(s) 1286
BseMII CTCAG 4 cut(s) 284, 804, 899, 1419
BseNI ACTGG 2 cut(s) 764, 1110
BseRI GAGGAG 3 cut(s) 630, 840, 1256
BseSI GKGCMC 2 cut(s) 298, 1195
BseXI GCAGC 5 cut(s) 472, 487, 562, 1426, 1512
Bsh1236I CGCG 1 cut(s) 15
BshFI GGCC 2 cut(s) 264, 1355
BshNI GGYRCC 1 cut(s) 35
BsiHKAI GWGCWC 3 cut(s) 298, 768, 1195
BsiSI CCGG 2 cut(s) 265, 1298
BslFI GGGAC 2 cut(s) 573, 1068
BslI CCNNNNNNNGG 1 cut(s) 1286
BsmAI GTCTC 2 cut(s) 174, 273
BsmFI GGGAC 2 cut(s) 573, 1068
BsnI GGCC 2 cut(s) 264, 1355
Bso31I GGTCTC 2 cut(s) 174, 273
Bsp119I TTCGAA 1 cut(s) 140
Bsp1286I GDGCHC 3 cut(s) 298, 768, 1195
Bsp143I GATC 1 cut(s) 1390
Bsp1720I GCTNAGC 1 cut(s) 908
BspACI CCGC 5 cut(s) 13, 33, 217, 722, 1295
BspANI GGCC 2 cut(s) 264, 1355
BspCNI CTCAG 4 cut(s) 283, 805, 900, 1420
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 6 cut(s) 37, 834, 1038, 1068, 1245, 1495
BspMAI CTGCAG 1 cut(s) 457
BspMI ACCTGC 1 cut(s) 1267
BspT104I TTCGAA 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 35
BspTNI GGTCTC 2 cut(s) 174, 273
BsrFI RCCGGY 2 cut(s) 264, 1297
BsrI ACTGG 2 cut(s) 764, 1110
BssAI RCCGGY 2 cut(s) 264, 1297
BssECI CCNNGG 4 cut(s) 239, 240, 277, 1338
BssMI GATC 1 cut(s) 1390
BssNI GRCGYC 2 cut(s) 36, 196
Bst2UI CCWGG 2 cut(s) 241, 278
Bst4CI ACNGT 5 cut(s) 85, 396, 680, 1082, 1190
Bst6I CTCTTC 2 cut(s) 420, 630
BstACI GRCGYC 2 cut(s) 36, 196
BstAPI GCANNNNNTGC 2 cut(s) 30, 302
BstBI TTCGAA 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 1035
BstDEI CTNAG 8 cut(s) 203, 270, 813, 908, 1176, 1428, 1457, 1470
BstENI CCTNNNNNAGG 1 cut(s) 1284
BstFNI CGCG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 4 cut(s) 17, 38, 707, 1279
BstKTI GATC 1 cut(s) 1393
BstMAI GTCTC 2 cut(s) 174, 273
BstMBI GATC 1 cut(s) 1390
BstMWI GCNNNNNNNGC 6 cut(s) 21, 30, 302, 325, 359, 943
BstNI CCWGG 2 cut(s) 241, 278
BstPAI GACNNNNGTC 1 cut(s) 1080
BstSCI CCNGG 2 cut(s) 239, 276
BstSFI CTRYAG 1 cut(s) 453
BstSLI GKGCMC 2 cut(s) 298, 1195
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 5 cut(s) 472, 487, 562, 1426, 1512
BstV2I GAAGAC 2 cut(s) 149, 1420
BstXI CCANNNNNNTGG 1 cut(s) 182
Bsu36I CCTNAGG 1 cut(s) 813
BsuRI GGCC 2 cut(s) 264, 1355
BtgZI GCGATG 1 cut(s) 1182
BtrI CACGTC 1 cut(s) 1331
BtsIMutI CAGTG 2 cut(s) 780, 1195
BveI ACCTGC 1 cut(s) 1267
Cac8I GCNNGC 1 cut(s) 1035
CaiI CAGNNNCTG 1 cut(s) 578
CfoI GCGC 4 cut(s) 17, 38, 707, 1279
Cfr10I RCCGGY 2 cut(s) 264, 1297
Cfr13I GGNCC 1 cut(s) 1353
CseI GACGC 1 cut(s) 1095
Csp6I GTAC 7 cut(s) 81, 226, 274, 397, 1256, 1290, 1511
CspCI CAANNNNNGTGG 4 cut(s) 652, 687, 883, 918
CviAII CATG 1 cut(s) 727
CviQI GTAC 7 cut(s) 81, 226, 274, 397, 1256, 1290, 1511
DdeI CTNAG 8 cut(s) 203, 270, 813, 908, 1176, 1428, 1457, 1470
DinI GGCGCC 1 cut(s) 37
DpnI GATC 1 cut(s) 1392
DpnII GATC 1 cut(s) 1390
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 2 cut(s) 420, 630
EarI CTCTTC 2 cut(s) 420, 630
EciI GGCGGA 1 cut(s) 1284
Eco31I GGTCTC 2 cut(s) 174, 273
Eco32I GATATC 1 cut(s) 742
Eco57I CTGAAG 1 cut(s) 1011
Eco81I CCTNAGG 1 cut(s) 813
EcoNI CCTNNNNNAGG 1 cut(s) 1284
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 2 cut(s) 239, 276
EcoRV GATATC 1 cut(s) 742
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
FaeI CATG 1 cut(s) 730
FaiI YATR 6 cut(s) 66, 215, 465, 728, 1004, 1508
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 2 cut(s) 573, 1068
FatI CATG 1 cut(s) 726
FauI CCCGC 1 cut(s) 210
Fnu4HI GCNGC 6 cut(s) 34, 486, 501, 576, 1440, 1501
Fsp4HI GCNGC 6 cut(s) 34, 486, 501, 576, 1440, 1501
FspBI CTAG 1 cut(s) 1401
GlaI GCGC 4 cut(s) 16, 37, 706, 1278
GluI GCNGC 6 cut(s) 34, 486, 501, 576, 1440, 1501
GsuI CTGGAG 1 cut(s) 837
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 2 cut(s) 264, 1355
HapII CCGG 2 cut(s) 265, 1298
HgaI GACGC 1 cut(s) 1095
HhaI GCGC 4 cut(s) 17, 38, 707, 1279
Hin1I GRCGYC 2 cut(s) 36, 196
Hin1II CATG 1 cut(s) 730
Hin6I GCGC 4 cut(s) 15, 36, 705, 1277
HinP1I GCGC 4 cut(s) 15, 36, 705, 1277
HincII GTYRAC 1 cut(s) 200
HindII GTYRAC 1 cut(s) 200
HinfI GANTC 3 cut(s) 841, 965, 1416
HpaII CCGG 2 cut(s) 265, 1298
HphI GGTGA 9 cut(s) 524, 596, 686, 692, 698, 728, 764, 1312, 1327
Hpy166II GTNNAC 4 cut(s) 200, 296, 1193, 1328
Hpy188I TCNGA 6 cut(s) 157, 610, 970, 1030, 1075, 1429
Hpy188III TCNNGA 9 cut(s) 59, 287, 336, 745, 812, 854, 918, 1210, 1401
Hpy8I GTNNAC 4 cut(s) 200, 296, 1193, 1328
Hpy99I CGWCG 2 cut(s) 44, 989
HpyCH4III ACNGT 5 cut(s) 85, 396, 680, 1082, 1190
HpyCH4IV ACGT 2 cut(s) 196, 1330
HpyCH4V TGCA 9 cut(s) 24, 296, 305, 392, 455, 488, 1055, 1193, 1366
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 30, 302, 325, 359, 943
HpyF3I CTNAG 8 cut(s) 203, 270, 813, 908, 1176, 1428, 1457, 1470
HpySE526I ACGT 2 cut(s) 196, 1330
Hsp92I GRCGYC 2 cut(s) 36, 196
Hsp92II CATG 1 cut(s) 730
HspAI GCGC 4 cut(s) 15, 36, 705, 1277
KasI GGCGCC 1 cut(s) 35
Kzo9I GATC 1 cut(s) 1390
LmnI GCTCC 5 cut(s) 497, 763, 853, 1042, 1243
Lsp1109I GCAGC 5 cut(s) 472, 487, 562, 1426, 1512
LweI GCATC 7 cut(s) 199, 337, 709, 1042, 1162, 1190, 1353
MaeI CTAG 1 cut(s) 1401
MaeII ACGT 2 cut(s) 196, 1330
MaeIII GTNAC 4 cut(s) 43, 118, 674, 1333
MalI GATC 1 cut(s) 1392
MboI GATC 1 cut(s) 1390
MhlI GDGCHC 3 cut(s) 298, 768, 1195
MluCI AATT 6 cut(s) 54, 151, 376, 774, 858, 1464
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 1 cut(s) 1410
MmeI TCCRAC 1 cut(s) 621
MroXI GAANNNNTTC 2 cut(s) 101, 604
MseI TTAA 3 cut(s) 53, 861, 1407
MslI CAYNNNNRTG 1 cut(s) 180
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 2 cut(s) 265, 1298
MspR9I CCNGG 2 cut(s) 241, 278
MvaI CCWGG 2 cut(s) 241, 278
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 6 cut(s) 21, 30, 302, 325, 359, 943
NarI GGCGCC 1 cut(s) 36
NdeII GATC 1 cut(s) 1390
NlaIII CATG 1 cut(s) 730
NlaIV GGNNCC 6 cut(s) 37, 834, 1038, 1068, 1245, 1495
NmeAIII GCCGAG 1 cut(s) 156
NmuCI GTSAC 4 cut(s) 43, 118, 674, 1333
NspV TTCGAA 1 cut(s) 140
PasI CCCWGGG 1 cut(s) 240
PcsI WCGNNNNNNNCGW 3 cut(s) 55, 128, 741
PdmI GAANNNNTTC 2 cut(s) 101, 604
PfeI GAWTC 2 cut(s) 841, 965
PkrI GCNGC 6 cut(s) 35, 487, 502, 577, 1441, 1502
PleI GAGTC 1 cut(s) 1410
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 1410
PshAI GACNNNNGTC 1 cut(s) 1080
Psp6I CCWGG 2 cut(s) 239, 276
PspGI CCWGG 2 cut(s) 239, 276
PspN4I GGNNCC 6 cut(s) 37, 834, 1038, 1068, 1245, 1495
PspPI GGNCC 1 cut(s) 1353
PsrI GAACNNNNNNTAC 2 cut(s) 1363, 1395
PstI CTGCAG 1 cut(s) 457
PstNI CAGNNNCTG 1 cut(s) 578
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 7 cut(s) 82, 227, 275, 398, 1257, 1291, 1512
RsaNI GTAC 7 cut(s) 81, 226, 274, 397, 1256, 1290, 1511
RseI CAYNNNNRTG 1 cut(s) 180
SaqAI TTAA 3 cut(s) 53, 861, 1407
SatI GCNGC 6 cut(s) 34, 486, 501, 576, 1440, 1501
Sau3AI GATC 1 cut(s) 1390
Sau96I GGNCC 1 cut(s) 1353
ScaI AGTACT 2 cut(s) 82, 1512
SchI GAGTC 1 cut(s) 1410
ScrFI CCNGG 2 cut(s) 241, 278
SduI GDGCHC 3 cut(s) 298, 768, 1195
SfaNI GCATC 7 cut(s) 199, 337, 709, 1042, 1162, 1190, 1353
SfcI CTRYAG 1 cut(s) 453
SfoI GGCGCC 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 140
SgrAI CRCCGGYG 1 cut(s) 1297
SmiMI CAYNNNNRTG 1 cut(s) 180
Sse9I AATT 6 cut(s) 54, 151, 376, 774, 858, 1464
SsiI CCGC 5 cut(s) 13, 33, 217, 722, 1295
SspDI GGCGCC 1 cut(s) 35
SspMI CTAG 1 cut(s) 1401
StyD4I CCNGG 2 cut(s) 239, 276
TaaI ACNGT 5 cut(s) 85, 396, 680, 1082, 1190
TaiI ACGT 2 cut(s) 199, 1333
TaqI TCGA 6 cut(s) 58, 73, 140, 744, 1114, 1448
TasI AATT 6 cut(s) 54, 151, 376, 774, 858, 1464
TatI WGTACW 4 cut(s) 80, 225, 396, 1510
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 2 cut(s) 841, 965
Tru1I TTAA 3 cut(s) 53, 861, 1407
Tru9I TTAA 3 cut(s) 53, 861, 1407
TscAI CASTG 2 cut(s) 787, 1195
TseFI GTSAC 4 cut(s) 43, 118, 674, 1333
TseI GCWGC 5 cut(s) 485, 500, 575, 1439, 1500
Tsp45I GTSAC 4 cut(s) 43, 118, 674, 1333
TspDTI ATGAA 9 cut(s) 94, 480, 494, 606, 645, 714, 807, 900, 1236
TspGWI ACGGA 1 cut(s) 1506
TspRI CASTG 2 cut(s) 787, 1195
VneI GTGCAC 2 cut(s) 294, 1191
XagI CCTNNNNNAGG 1 cut(s) 1284
XapI RAATTY 3 cut(s) 151, 774, 1464
XbaI TCTAGA 1 cut(s) 1400
XmnI GAANNNNTTC 2 cut(s) 101, 604
XspI CTAG 1 cut(s) 1401
ZraI GACGTC 1 cut(s) 197
ZrmI AGTACT 2 cut(s) 82, 1512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.