pycom06g09180

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002430
Physical Location & Seq
Forward (+)
14397681 .. 14398981
1301 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGGCGGAGCGTACGGACGGAGTGAAGGCGGCGGAGTCTTTGATGGACAGGATATCCGACAAGATCCACGGCCACGATTCGTCGTCGGTGGTGCCGTCGTCGGGGGTGGTGCACGACAAGGGCATTGGCGGTGGCCATTCAACGTCGTTGAAGTCGAAGGTTAACAGGCTCTTTGGGAGGGAGAAGCCCGTGCACCACGTCTTCGGTGGCGGAAAAGCGGCTGATGTTTTCCTGTGGAGGGACAAGAAGGCGTCCGCAGGTGTTCTTATCGCCTTAACGATCATATGGGTCTTCTTTGAATTGTTTGAATATCACCTGCTGACTCTGGTCTCCCACTTGTTGATTCTTGGGATTGCTTCGTTGTTCTTATGGTCCAACGCTTCAACCTTCATTAACAAGTCTCCACCCCACATTCCACAAGTTCAAATTCCAGAAAAGCCGGTTCTACAGATTGCGTCTGTAATCACAATGGAGATCAATCGGGCTTTTGTTATCCTGCGGGAGATTGCTTCTGGCAAGGATCTGAAGCAGTTCCTCTCTGTATGTTTGTTGTGCTCTAGCTCTTTCTTTTCTAATTGA

Protein Analysis

193

Amino Acids

21.02

Weight (kDa)

9.17

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 74 - 184 2.9e-34 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 248, 324
Acc36I ACCTGC 2 cut(s) 248, 324
AccB1I GGYRCC 1 cut(s) 91
AciI CCGC 8 cut(s) 5, 29, 32, 129, 210, 218, 255, 499
AclWI GGATC 2 cut(s) 58, 528
AcoI YGGCCR 2 cut(s) 70, 133
AcsI RAATTY 1 cut(s) 426
AcuI CTGAAG 1 cut(s) 545
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 2 cut(s) 101, 238
AgsI TTSAA 6 cut(s) 141, 151, 299, 308, 384, 425
AjiI CACGTC 1 cut(s) 199
AluBI AGCT 1 cut(s) 561
AluI AGCT 1 cut(s) 561
Alw21I GWGCWC 3 cut(s) 114, 195, 557
Alw26I GTCTC 2 cut(s) 334, 405
Alw44I GTGCAC 2 cut(s) 110, 191
AlwI GGATC 2 cut(s) 58, 528
AoxI GGCC 2 cut(s) 70, 133
ApaLI GTGCAC 2 cut(s) 110, 191
ApoI RAATTY 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 530
AspS9I GGNCC 1 cut(s) 372
AsuHPI GGTGA 1 cut(s) 305
AvaII GGWCC 1 cut(s) 372
BaeGI GKGCMC 2 cut(s) 114, 195
BalI TGGCCA 1 cut(s) 135
BanI GGYRCC 1 cut(s) 91
BbsI GAAGAC 2 cut(s) 193, 283
Bbv12I GWGCWC 3 cut(s) 114, 195, 557
BccI CCATC 1 cut(s) 37
BceAI ACGGC 2 cut(s) 79, 85
BcoDI GTCTC 2 cut(s) 334, 405
BfaI CTAG 1 cut(s) 558
BfmI CTRYAG 1 cut(s) 446
BfuAI ACCTGC 2 cut(s) 248, 324
BisI GCNGC 2 cut(s) 30, 219
BlsI GCNGC 2 cut(s) 31, 220
Bme18I GGWCC 1 cut(s) 372
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 372
BmiI GGNNCC 1 cut(s) 93
BoxI GACNNNNGTC 1 cut(s) 326
BpiI GAAGAC 2 cut(s) 193, 283
BsaHI GRCGYC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 1 cut(s) 67
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 238
Bse118I RCCGGY 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 67
BseLI CCNNNNNNNGG 2 cut(s) 101, 238
BseSI GKGCMC 2 cut(s) 114, 195
BshFI GGCC 2 cut(s) 72, 135
BshNI GGYRCC 1 cut(s) 91
BsiHKAI GWGCWC 3 cut(s) 114, 195, 557
BsiSI CCGG 1 cut(s) 440
BsiWI CGTACG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 101, 238
BsmAI GTCTC 2 cut(s) 334, 405
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 2 cut(s) 72, 135
Bso31I GGTCTC 1 cut(s) 334
Bsp1286I GDGCHC 3 cut(s) 114, 195, 557
Bsp143I GATC 4 cut(s) 63, 279, 474, 520
BspACI CCGC 8 cut(s) 5, 29, 32, 129, 210, 218, 255, 499
BspANI GGCC 2 cut(s) 72, 135
BspLI GGNNCC 1 cut(s) 93
BspMI ACCTGC 2 cut(s) 248, 324
BspPI GGATC 2 cut(s) 58, 528
BspT107I GGYRCC 1 cut(s) 91
BspTNI GGTCTC 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 439
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 4 cut(s) 63, 279, 474, 520
BssNI GRCGYC 1 cut(s) 251
BstACI GRCGYC 1 cut(s) 251
BstDSI CCRYGG 1 cut(s) 67
BstKTI GATC 4 cut(s) 66, 282, 477, 523
BstMAI GTCTC 2 cut(s) 334, 405
BstMBI GATC 4 cut(s) 63, 279, 474, 520
BstPAI GACNNNNGTC 1 cut(s) 326
BstSFI CTRYAG 1 cut(s) 446
BstSLI GKGCMC 2 cut(s) 114, 195
BstV2I GAAGAC 2 cut(s) 193, 283
BstX2I RGATCY 2 cut(s) 63, 520
BstYI RGATCY 2 cut(s) 63, 520
BsuRI GGCC 2 cut(s) 72, 135
BtgI CCRYGG 1 cut(s) 67
BtrI CACGTC 1 cut(s) 199
BveI ACCTGC 2 cut(s) 248, 324
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 372
CseI GACGC 2 cut(s) 240, 444
Csp6I GTAC 1 cut(s) 12
CviJI RGCY 8 cut(s) 72, 135, 169, 187, 221, 439, 485, 561
CviKI_1 RGCY 8 cut(s) 72, 135, 169, 187, 221, 439, 485, 561
CviQI GTAC 1 cut(s) 12
DpnI GATC 4 cut(s) 65, 281, 476, 522
DpnII GATC 4 cut(s) 63, 279, 474, 520
EaeI YGGCCR 2 cut(s) 70, 133
EciI GGCGGA 3 cut(s) 20, 47, 225
Eco31I GGTCTC 1 cut(s) 334
Eco32I GATATC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 545
EcoRV GATATC 1 cut(s) 54
FaiI YATR 4 cut(s) 284, 286, 370, 544
FaqI GGGAC 1 cut(s) 254
FauI CCCGC 1 cut(s) 492
FauNDI CATATG 1 cut(s) 284
Fnu4HI GCNGC 2 cut(s) 30, 219
Fsp4HI GCNGC 2 cut(s) 30, 219
FspBI CTAG 1 cut(s) 558
GluI GCNGC 2 cut(s) 30, 219
HaeIII GGCC 2 cut(s) 72, 135
HapII CCGG 1 cut(s) 440
HgaI GACGC 2 cut(s) 240, 444
Hin1I GRCGYC 1 cut(s) 251
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 4 cut(s) 35, 77, 322, 343
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 1 cut(s) 440
HphI GGTGA 1 cut(s) 305
Hpy166II GTNNAC 3 cut(s) 112, 163, 193
Hpy188I TCNGA 2 cut(s) 58, 525
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 3 cut(s) 112, 163, 193
Hpy99I CGWCG 5 cut(s) 85, 88, 100, 103, 148
HpyAV CCTTC 4 cut(s) 19, 151, 241, 397
HpyCH4IV ACGT 2 cut(s) 143, 198
HpyCH4V TGCA 2 cut(s) 112, 193
HpySE526I ACGT 2 cut(s) 143, 198
Hsp92I GRCGYC 1 cut(s) 251
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 4 cut(s) 63, 279, 474, 520
LmnI GCTCC 1 cut(s) 7
MaeI CTAG 1 cut(s) 558
MaeII ACGT 2 cut(s) 143, 198
MalI GATC 4 cut(s) 65, 281, 476, 522
MboI GATC 4 cut(s) 63, 279, 474, 520
MboII GAAGA 2 cut(s) 193, 283
MflI RGATCY 2 cut(s) 63, 520
MhlI GDGCHC 3 cut(s) 114, 195, 557
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 3 cut(s) 299, 426, 574
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 2 cut(s) 44, 316
MmeI TCCRAC 2 cut(s) 81, 399
MnlI CCTC 3 cut(s) 171, 231, 545
Mox20I TGGCCA 1 cut(s) 135
MroXI GAANNNNTTC 1 cut(s) 530
MscI TGGCCA 1 cut(s) 135
MseI TTAA 3 cut(s) 162, 275, 393
Msp20I TGGCCA 1 cut(s) 135
MspI CCGG 1 cut(s) 440
NdeI CATATG 1 cut(s) 284
NdeII GATC 4 cut(s) 63, 279, 474, 520
NlaIV GGNNCC 1 cut(s) 93
PaqCI CACCTGC 2 cut(s) 248, 324
PcsI WCGNNNNNNNCGW 2 cut(s) 92, 152
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 77, 343
Pfl23II CGTACG 1 cut(s) 11
PkrI GCNGC 2 cut(s) 31, 220
PleI GAGTC 2 cut(s) 43, 316
PpsI GAGTC 2 cut(s) 43, 316
PshAI GACNNNNGTC 1 cut(s) 326
PspLI CGTACG 1 cut(s) 11
PspN4I GGNNCC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 372
PsuI RGATCY 2 cut(s) 63, 520
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 162, 275, 393
SatI GCNGC 2 cut(s) 30, 219
Sau3AI GATC 4 cut(s) 63, 279, 474, 520
Sau96I GGNCC 1 cut(s) 372
SchI GAGTC 2 cut(s) 44, 316
SduI GDGCHC 3 cut(s) 114, 195, 557
SetI ASST 7 cut(s) 146, 162, 201, 262, 318, 389, 563
SfcI CTRYAG 1 cut(s) 446
SinI GGWCC 1 cut(s) 372
Sse9I AATT 3 cut(s) 299, 426, 574
SsiI CCGC 8 cut(s) 5, 29, 32, 129, 210, 218, 255, 499
SspMI CTAG 1 cut(s) 558
TaiI ACGT 2 cut(s) 146, 201
TaqI TCGA 1 cut(s) 155
TasI AATT 3 cut(s) 299, 426, 574
TauI GCSGC 2 cut(s) 32, 221
TfiI GAWTC 2 cut(s) 77, 343
Tru1I TTAA 3 cut(s) 162, 275, 393
Tru9I TTAA 3 cut(s) 162, 275, 393
TspDTI ATGAA 1 cut(s) 379
TspGWI ACGGA 2 cut(s) 29, 33
VneI GTGCAC 2 cut(s) 110, 191
VpaK11BI GGWCC 1 cut(s) 372
XapI RAATTY 1 cut(s) 426
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.