pycom08g10330

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003666
Physical Location & Seq
Reverse (-)
8671991 .. 8674056
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
ATGGCTTCTTCCGACATGGATCACAACGCCCCTCACTCTATGGGTACCACCATCATCGGCGTCACCTACGACGGAGGTGTCGTCCTTGGCGCCGACTCTCGTACCAGCACCGGAGTATACGTGGCCAATCGCGCCTCCGACAAAATCACCCAGCTCACCGACAATGTCTACGTCTGCCGCTCTGGATCGGCTGCCGATTCCCAGGTTGTTTCCGATTACGTTCGTTACTTCCTTCATCAGCACACTCAGCTTGGACAACCTGCGACCGTCAAAGTTTGTGCAAACCTCGTCAGGCTGCTGTCCTATGGTAACAAGAATATGTTGGAAACTGGTCTGATTGTTGGCGGGTGGGACAAGTACGAAGGCGGTAAGATTTATGGGATTCCTCTTGGTGGCACACTGCTAGAGCTGCCCTTTGCCATTGGAGGATCTGGCTCCAGTTACTTGTATGGATTTATCGATCAAGCATGGAAAGAAGGAATGACCAAGGACGAAGCTGAGCAATTGGTGGTTAAGGCTGTTTCCCTCGCCATTGCACGAGATGGTGCCAGTGGGGGTGTTGTCCGTACTGTAGTTATAAATTCTGAGGGAGTGACAAGAAACTACTATCCTGGCGACAAACTTCCACTCTGGCATGAGGAGTTGGAGCCTCAGAACTCATTGTTGGACATATTGAACACTGCTAGTCCCGAGCCAATGAACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

236

Amino Acids

25.19

Weight (kDa)

5.05

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 14 - 193 2.4e-47 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 578
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 268
Acc65I GGTACC 1 cut(s) 44
AccB1I GGYRCC 3 cut(s) 44, 89, 545
AccBSI CCGCTC 1 cut(s) 180
AccI GTMKAC 2 cut(s) 117, 168
AccII CGCG 1 cut(s) 132
AciI CCGC 3 cut(s) 178, 345, 366
AclWI GGATC 3 cut(s) 27, 193, 436
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 1 cut(s) 580
AcyI GRCGYC 2 cut(s) 60, 90
AfaI GTAC 4 cut(s) 46, 103, 359, 568
AfiI CCNNNNNNNGG 1 cut(s) 392
AgsI TTSAA 1 cut(s) 676
AjnI CCWGG 2 cut(s) 201, 610
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
AluBI AGCT 4 cut(s) 154, 250, 409, 497
AluI AGCT 4 cut(s) 154, 250, 409, 497
AlwI GGATC 3 cut(s) 27, 193, 436
Ama87I CYCGRG 1 cut(s) 689
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 3 cut(s) 191, 295, 409
ApoI RAATTY 1 cut(s) 580
Asp718I GGTACC 1 cut(s) 44
AspLEI GCGC 2 cut(s) 92, 134
AsuHPI GGTGA 3 cut(s) 55, 139, 148
AvaI CYCGRG 1 cut(s) 689
BaeI ACNNNNGTAYC 2 cut(s) 85, 118
BalI TGGCCA 1 cut(s) 125
BanI GGYRCC 3 cut(s) 44, 89, 545
BauI CACGAG 1 cut(s) 537
BbvI GCAGC 3 cut(s) 178, 282, 396
BccI CCATC 2 cut(s) 59, 536
BciT130I CCWGG 2 cut(s) 203, 612
BfaI CTAG 2 cut(s) 404, 684
BfmI CTRYAG 1 cut(s) 570
BfoI RGCGCY 1 cut(s) 93
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 4 cut(s) 178, 192, 296, 410
BlpI GCTNAGC 1 cut(s) 498
BlsI GCNGC 4 cut(s) 179, 193, 297, 411
Bme1390I CCNGG 2 cut(s) 203, 612
BmeT110I CYCGRG 1 cut(s) 689
BmiI GGNNCC 5 cut(s) 46, 91, 436, 547, 648
BmrFI CCNGG 2 cut(s) 203, 612
BpmI CTGGAG 1 cut(s) 421
Bpu1102I GCTNAGC 1 cut(s) 498
Bsa29I ATCGAT 1 cut(s) 459
BsaAI YACGTR 1 cut(s) 121
BsaHI GRCGYC 2 cut(s) 60, 90
BsaJI CCNNGG 3 cut(s) 85, 201, 486
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 105, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse1I ACTGG 3 cut(s) 334, 438, 549
Bse3DI GCAATG 1 cut(s) 531
BseBI CCWGG 2 cut(s) 203, 612
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 85, 201, 486
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 4 cut(s) 260, 489, 576, 665
BseNI ACTGG 3 cut(s) 334, 438, 549
BseRI GAGGAG 1 cut(s) 653
BseXI GCAGC 3 cut(s) 178, 282, 396
BseYI CCCAGC 1 cut(s) 150
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 1 cut(s) 125
BshNI GGYRCC 3 cut(s) 44, 89, 545
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 2 cut(s) 365, 672
BslI CCNNNNNNNGG 1 cut(s) 392
BsmFI GGGAC 2 cut(s) 365, 672
BsnI GGCC 1 cut(s) 125
BsoBI CYCGRG 1 cut(s) 689
Bsp143I GATC 4 cut(s) 19, 185, 428, 460
Bsp1720I GCTNAGC 1 cut(s) 498
BspACI CCGC 3 cut(s) 178, 345, 366
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 4 cut(s) 259, 490, 577, 664
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 5 cut(s) 46, 91, 436, 547, 648
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 3 cut(s) 27, 193, 436
BspT107I GGYRCC 3 cut(s) 44, 89, 545
BsrBI CCGCTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 531
BsrI ACTGG 3 cut(s) 334, 438, 549
BssECI CCNNGG 3 cut(s) 85, 201, 486
BssMI GATC 4 cut(s) 19, 185, 428, 460
BssNAI GTATAC 1 cut(s) 118
BssNI GRCGYC 2 cut(s) 60, 90
BssSI CACGAG 1 cut(s) 537
BssT1I CCWWGG 2 cut(s) 85, 486
Bst1107I GTATAC 1 cut(s) 118
Bst2BI CACGAG 1 cut(s) 537
Bst2UI CCWGG 2 cut(s) 203, 612
Bst4CI ACNGT 2 cut(s) 268, 571
BstACI GRCGYC 2 cut(s) 60, 90
BstBAI YACGTR 1 cut(s) 121
BstDEI CTNAG 4 cut(s) 246, 498, 585, 651
BstFNI CGCG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 134
BstKTI GATC 4 cut(s) 22, 188, 431, 463
BstMBI GATC 4 cut(s) 19, 185, 428, 460
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 3 cut(s) 131, 247, 409
BstNI CCWGG 2 cut(s) 203, 612
BstSCI CCNGG 2 cut(s) 201, 610
BstSFI CTRYAG 1 cut(s) 570
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 178, 282, 396
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BstZ17I GTATAC 1 cut(s) 118
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 1 cut(s) 125
BsuTUI ATCGAT 1 cut(s) 459
BtsI GCAGTG 2 cut(s) 398, 678
BtsIMutI CAGTG 3 cut(s) 398, 556, 678
BveI ACCTGC 1 cut(s) 268
CfoI GCGC 2 cut(s) 92, 134
ClaI ATCGAT 1 cut(s) 459
CseI GACGC 1 cut(s) 49
Csp6I GTAC 4 cut(s) 45, 102, 358, 567
CviAII CATG 3 cut(s) 16, 468, 635
CviQI GTAC 4 cut(s) 45, 102, 358, 567
DdeI CTNAG 4 cut(s) 246, 498, 585, 651
DinI GGCGCC 1 cut(s) 91
DpnI GATC 4 cut(s) 21, 187, 430, 462
DpnII GATC 4 cut(s) 19, 185, 428, 460
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 123
Eco130I CCWWGG 2 cut(s) 85, 486
Eco88I CYCGRG 1 cut(s) 689
EcoRII CCWGG 2 cut(s) 201, 610
EcoT14I CCWWGG 2 cut(s) 85, 486
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 2 cut(s) 85, 486
FaeI CATG 3 cut(s) 19, 471, 638
FaqI GGGAC 2 cut(s) 365, 672
FatI CATG 3 cut(s) 15, 467, 634
FauI CCCGC 1 cut(s) 338
FauNDI CATATG 1 cut(s) 704
FblI GTMKAC 2 cut(s) 117, 168
Fnu4HI GCNGC 4 cut(s) 178, 192, 296, 410
Fsp4HI GCNGC 4 cut(s) 178, 192, 296, 410
FspBI CTAG 2 cut(s) 404, 684
GlaI GCGC 2 cut(s) 91, 133
GluI GCNGC 4 cut(s) 178, 192, 296, 410
GsaI CCCAGC 1 cut(s) 154
GsuI CTGGAG 1 cut(s) 421
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 1 cut(s) 125
HapII CCGG 1 cut(s) 111
HgaI GACGC 1 cut(s) 49
HhaI GCGC 2 cut(s) 92, 134
Hin1I GRCGYC 2 cut(s) 60, 90
Hin1II CATG 3 cut(s) 19, 471, 638
Hin6I GCGC 2 cut(s) 90, 132
HinP1I GCGC 2 cut(s) 90, 132
HinfI GANTC 3 cut(s) 95, 197, 382
HpaII CCGG 1 cut(s) 111
HphI GGTGA 3 cut(s) 55, 139, 148
Hpy166II GTNNAC 2 cut(s) 118, 169
Hpy188I TCNGA 6 cut(s) 13, 139, 214, 336, 586, 654
Hpy188III TCNNGA 2 cut(s) 183, 689
Hpy8I GTNNAC 2 cut(s) 118, 169
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 3 cut(s) 242, 356, 470
HpyCH4III ACNGT 2 cut(s) 268, 571
HpyCH4IV ACGT 3 cut(s) 120, 171, 219
HpyCH4V TGCA 2 cut(s) 281, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 247, 409
HpyF3I CTNAG 4 cut(s) 246, 498, 585, 651
HpySE526I ACGT 3 cut(s) 120, 171, 219
Hsp92I GRCGYC 2 cut(s) 60, 90
Hsp92II CATG 3 cut(s) 19, 471, 638
HspAI GCGC 2 cut(s) 90, 132
KasI GGCGCC 1 cut(s) 89
KpnI GGTACC 1 cut(s) 48
Kzo9I GATC 4 cut(s) 19, 185, 428, 460
LmnI GCTCC 2 cut(s) 440, 646
Lsp1109I GCAGC 3 cut(s) 178, 282, 396
MaeI CTAG 2 cut(s) 404, 684
MaeII ACGT 3 cut(s) 120, 171, 219
MaeIII GTNAC 5 cut(s) 61, 224, 308, 440, 592
MalI GATC 4 cut(s) 21, 187, 430, 462
MbiI CCGCTC 1 cut(s) 180
MboI GATC 4 cut(s) 19, 185, 428, 460
MfeI CAATTG 1 cut(s) 503
MflI RGATCY 1 cut(s) 428
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 2 cut(s) 503, 580
MluNI TGGCCA 1 cut(s) 125
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 5 cut(s) 36, 162, 303, 624, 645
Mox20I TGGCCA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 125
MseI TTAA 1 cut(s) 513
Msp20I TGGCCA 1 cut(s) 125
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 2 cut(s) 203, 612
MunI CAATTG 1 cut(s) 503
MvaI CCWGG 2 cut(s) 203, 612
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 3 cut(s) 131, 247, 409
NarI GGCGCC 1 cut(s) 90
NdeI CATATG 1 cut(s) 704
NdeII GATC 4 cut(s) 19, 185, 428, 460
NlaIII CATG 3 cut(s) 19, 471, 638
NlaIV GGNNCC 5 cut(s) 46, 91, 436, 547, 648
NmuCI GTSAC 2 cut(s) 61, 592
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PfeI GAWTC 2 cut(s) 197, 382
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 4 cut(s) 179, 193, 297, 411
PleI GAGTC 1 cut(s) 89
PluTI GGCGCC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 121
PsiI TTATAA 1 cut(s) 578
Psp6I CCWGG 2 cut(s) 201, 610
PspFI CCCAGC 1 cut(s) 150
PspGI CCWGG 2 cut(s) 201, 610
PspN4I GGNNCC 5 cut(s) 46, 91, 436, 547, 648
PsuI RGATCY 1 cut(s) 428
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 4 cut(s) 46, 103, 359, 568
RsaNI GTAC 4 cut(s) 45, 102, 358, 567
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 4 cut(s) 178, 192, 296, 410
Sau3AI GATC 4 cut(s) 19, 185, 428, 460
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 203, 612
SfcI CTRYAG 1 cut(s) 570
SfoI GGCGCC 1 cut(s) 91
Sse9I AATT 2 cut(s) 503, 580
SsiI CCGC 3 cut(s) 178, 345, 366
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 2 cut(s) 404, 684
StyD4I CCNGG 2 cut(s) 201, 610
StyI CCWWGG 2 cut(s) 85, 486
TaaI ACNGT 2 cut(s) 268, 571
TaiI ACGT 3 cut(s) 123, 174, 222
TaqI TCGA 1 cut(s) 459
TasI AATT 2 cut(s) 503, 580
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 2 cut(s) 197, 382
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TscAI CASTG 3 cut(s) 405, 556, 685
TseFI GTSAC 2 cut(s) 61, 592
TseI GCWGC 3 cut(s) 191, 295, 409
Tsp45I GTSAC 2 cut(s) 61, 592
TspDTI ATGAA 1 cut(s) 224
TspGWI ACGGA 2 cut(s) 87, 554
TspRI CASTG 3 cut(s) 405, 556, 685
Tth111I GACNNNGTC 1 cut(s) 164
XapI RAATTY 1 cut(s) 580
XmiI GTMKAC 2 cut(s) 117, 168
XspI CTAG 2 cut(s) 404, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.