Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0003176

Physical Location & Seq

Genomic Coordinates Reverse (-)

5749934 .. 5753880

Gene Structure 3947 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3627 bp

ATGAAATCCGCCAACATCAAAACCCTAGATTTCACCAGGCAACCCTTTATCGAACCCGATACGCACCGTACAGTTAAGGAACTGAAATTTATCGTTATGTCCCCGGCAGAGATTATGAAACTGGCGGAAGTTCAAATCTACATTGGCACATATTACAACTCCGGTTGGAAACCCATCGAAGGCGGCTTACTGGACCCGCGGCTAAGCCGGGCGAGCAAGCGGTTAATCTGCGCCACTTGTGAAGGGAACCACATCGAGTGCCCGGGGCATTTTGGGTATTTGGCCCTCGCCCTCCCCGTCTACAACGTCGGGTATCTGGGCACGGTTATGAACGTCCTGAAGTGTATCTGCAAGAATTGTTCTGGGTTTCTACTGAAGGACGAAATGCGGGTGGAGTATTTGGGGGAGATGAGGAATCTAAAGGATCCTATGAAAAAAATGGAACTGATGAAAATTGCGGTGAAGAAATGTACTGCATTGTGTGCCGGTAATCGGCGGACGGAGTGCTGGAAATGCGGGTATGTGAACGGGACGGTGAAGAAGGCGGTTGGGATGATCGGCATTCTTCACGACCGTTCGAAATTTGAAGATGTGATGGATGAGTTGAGAAAAACAATGGTGGGGACTAAAGTCTCTGCAGCGGGGTTGAGCGTGGAAGCTATGTTAGACCCCATAAAAGTTTTCACTTTGTTGAAAGAGATGAGTGATAAGGATTGTGAGGTGCTTTATTTGTCTCCTAGGCCGGAGAATCTCTTGATAACCAACATTCTGGTGCCGCCTACTGTGATTCACCCTTCAGTTTTTGTTGGGGGAGAAGTGAGCAATGAGAATGACATTACGGTGAGGTTGAAGCAGATCATTCAGTCGAATGCGCGCCTTACGCGCGATTTGTTTGATTTGAGGCTGGGGTATGCCCCCAGGGTTAAGCGGAGTTGGGATGAACTTCAGGTTGAGGTTACACAGTATATAAATAGTGAGGTTCGTGTGCCTTTCACGATGCAAGGTGGGAATCCGTTGAAAGGTTTTGTGCAGTGGCTCAAGGGGAAGCAAGGGCGTTTTCGCGGGAATTTGTCTGCGAAACGTGTTGAATATACTGGAAGAAGTGTTATATCTCTTGACCCTAATCTAAAAATCAATGAGGTTGGAGTCCCCATTGAAATGGCTCAAAGCTTAACGTACCTCGAATGGGTTACATGTCATAATATTGAGAAGTTGAGGAAGTGTGTGTGTGATGGACCTCATAAGTACCCCGGTGCCAGATCTTTGCATCGTGATGGTGAAGAATTCTCCTTGGCGATTACGAGGATCAGTAAAAATCTGGTTGATGGACTCCAATACGGTGACATAGTTGGACGCCATCTAGAAGACGGTGACATTCTTCTTTTCAACAGACAACCAAGCCTGCATAGGATGTCTTTCATGTCTCATCGGGCTAAGGTTATGCCTTGGAGGACGCTGAGGTTTAATGAATCTGTTTGCAATCCGTATAATGCTGATTTTGACGGCGATGAGATGAACATTCATGTCCCGCAAACAGAGGAGGCGTGGGCAGAGGCTGCTCTGTTGTTGGGGGTGGAAAACAATTTGTGCACACCCAAAAATGGGGAGATCTTGGTTGCTTCAACTCAAGATTTTCTGACATCATCTTTCCTTATAACGAGGAAGGACACATTTTATGACCGTTCATCATTTTCACTCATGTGTTCATATATGGGGAGTGTAGAGTTGTGGACAGGAAAGCAATTGTTTAGTGTTCTCGTACGCCCGAATGTAAACCTGAGAGTCTATCTGAATCTAATTGTCACAGAGAAGTCCTACACCACCACCAAGACCGAAGATGGGAGAGAAATTGAAGCAATGTGCCCGAAAGATGGGTTTGTTTATTTTCGTAACAGTGTGCTCATAGCCGGGCAACTTGGGAAGGCTACATTGGGAAATGGCAACAAGAATGGACTTTACTCTGCTCTTCTCAGAGACTATAAACCTCAGGCAGCTGTTGCTTGCATGAATTGCTTAGCTAAGTTAAGCGCGCAATGGATTGGTAACCATGGATTCTCAATTGGGATAAGTGATGTTCAGCCAAGTGGCAATTTGCGCGAAGAGAAAAAGAAGATAATTAAGGAAGGCTACGAAAAATGTTATGGCCAGATAAAGTTGTTCAATGAAGGGCAGCTCCAACTTATATCAGGTTCTGATGCAGCATATCCCTTGATCATGTCCCAATGTGGTTCTAAAGGATCTGCCAAAAATATAAGCCAGATGATTGTTTGTGGTTGCCAGCAATCTGTTGGTGGTTGTCGCGCCCCCAATGGGTTCATAGACCGAAGCCTTCCACACTTCCTTAGAGGTGTAAAAACCCCTGCCGCTAAAGGGTTTGTTGCTAGCTCATTTTATAGCGGCTTGAGTCCTACAGAGTTCTTCTTCCATACAATGGGAGGGTACCTAAGTCATAGATTGACCAAAGTTATGGAGGATCTATGTGTTCAGTATGATAAAACAGTGAGAAATGCGAGTGGATGTATACTTCAGTTCTGTTATGGAGATGATGGTATGGATCCTGCAGTGATGGAAGAAAAAGATGAAGCTCCGTTGGACTTGCCCCGGTTGTTTTTGAAAGCGAAAGCCACATGTCTTGCCGATAAAAATGAGCTTTCTGAACCAGATATGACTCCTGAGGCTGGGTGCTCTGCCGGTTTCAAAACTTCTTTGGAAGAATTTTTGGATGAGTATGTAGAATCATTCATAAAAAAACATGAGATATTTATGTTTGATGATGATCAAATCCCTGCTTGGAAGGAGAAGATTGTGCAAAATATTGCGGGTGTTACTTCTAGACAGTTGGAGGTTTTTCTTGATACTTGCATTTCACGTTACCATTCGAAGAAGATTGAGGGTGGAACAACAATTGGAATAATCGGAGCTCAAAGTATTTGCGAGCCTGCGACGCAGATGACATTGAAAACTTTCCACTTTGCTGGAGTTAACATCCACACGTCAATCATCACTGCGGCGCTCGAATGTGATACTAATGAAAAATCTGCAAGGGTGGTAGCAGGGCGGATTGAGAACACAAGTCTCGGGCAAATTGCTGAAAGTATACAGATTGTAATGACTGCAAGATCAGTGTCTATAGTGATCACGCTCGATATGTCCATGATTCAAGATGCGCACTTGTCTGTGGATGCTAATATGGTGAAAGAATCGATTTTACAAATTCGGGGAATCAAACTGAAGTCAGAAAATGTTAAGGTTTTGGATATTAGAAAATTGGAAGTTTTTCCTGCAGAGACTGACAGGAGCAAACTTTATTTTCGCCTTCACAGCTTAAAGAACAGGTTCTCATCAATCACACTAAGGGGCATAAACACAGTCAAGCGTGTCATGATTAACAACGAAAAAGAAATGAAAACCAATCACAAGAAGTACAAGTTGTTCGCTGAAAGCAGAGGGCTTCTAGAAGTGATGGGGACTAAAGGAGTAAACGGATGCAGAACCACAAGCAATGACGTTCTGAAAGTGCAGAGGACGCTTGGGATCGAAGCGGCACGAGTCTGCATCATTGAGGAGATAAAGAGCACGATGAAACAACATGGGATGAACATAGACTGGGGGAAGTGCTTCGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002376 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0046483 GO:0048518 GO:0050789 GO:0050896 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1209

Amino Acids

135.2

Weight (kDa)

8.64

Isoelectric Point (pI)

39.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	23 - 357	1.7e-68	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	360 - 524	6.6e-65	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	528 - 692	1.7e-31	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	735 - 793	1.2e-14	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	800 - 1202	4.2e-43	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60040 AT5G60040
fragaria_vesca	FvH4_3g34290 FvH4_3g34290 FvH4_3g34290 FvH4_6g15631
malus_domestica	MD03G1106800.v1.1 MD09G1157000.v1.1
prunus_persica	Prupe.6G089600_v2.0.a1 Prupe.6G089600_v2.0.a1
pyrus_communis	pycom03g08620 pycom09g07570
rosa_chinensis	RchiOBHm_Chr3g0469301 RchiOBHm_Chr3g0469321 RchiOBHm_Chr5g0061861
rosa_laevigata	RLG00000024360 RLG00000035511
rosa_multiflora	Rmu_sc0004134.1_g000001 Rmu_sc0011641.1_g000013 Rmu_sc0011641.1_g000015 Rmu_sc0019271.1_g000001 Rmu_sc0022191.1_g000001
rosa_roxburghii	Rroxscaffold_1G00018870 Rroxscaffold_6G00411990 Rroxscaffold_6G00412000
rosa_rugosa	Rorug03G0100400 Rorug05G0344900 Rorug05G0345000
rosa_samantha	Rh3AG149800 Rh3BG173400 Rh3CG164500 Rh3DG202000 Rh5AG405500 Rh5BG418300 Rh5CG442800 Rh5DG432400
rosa_wichuraiana	Rw5G038070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1655
Acc16I	TGCGCA	1 cut(s)	3168
Acc65I	GGTACC	1 cut(s)	2439
AccB1I	GGYRCC	3 cut(s)	772, 1253, 2439
AccB7I	CCANNNNNTGG	1 cut(s)	2431
AccI	GTMKAC	3 cut(s)	300, 2521, 3097
AccII	CGCG	8 cut(s)	199, 874, 883, 885, 1062, 2030, 2097, 2301
AclWI	GGATC	8 cut(s)	419, 432, 1313, 2245, 2481, 2549, 2562, 3542
AcoI	YGGCCR	1 cut(s)	2143
AcsI	RAATTY	6 cut(s)	86, 581, 1066, 1283, 2714, 3213
AcuI	CTGAAG	6 cut(s)	359, 395, 780, 929, 2510, 3251
AcyI	GRCGYC	1 cut(s)	1354
AfaI	GTAC	7 cut(s)	70, 472, 1178, 1247, 1761, 2441, 3425
AflIII	ACRYGT	4 cut(s)	1081, 1193, 2627, 2991
AjiI	CACGTC	1 cut(s)	2994
AjnI	CCWGG	2 cut(s)	35, 917
AjuI	GAANNNNNNNTTGG	2 cut(s)	1913, 1945
AleI	CACNNNNGTG	1 cut(s)	1699
Alw21I	GWGCWC	5 cut(s)	1592, 1902, 2687, 2923, 3578
Alw26I	GTCTC	6 cut(s)	637, 738, 1428, 1968, 3080, 3281
Alw44I	GTGCAC	1 cut(s)	1588
AlwI	GGATC	8 cut(s)	419, 432, 1313, 2245, 2481, 2549, 2562, 3542
AlwNI	CAGNNNCTG	3 cut(s)	1556, 2192, 3290
Ama87I	CYCGRG	2 cut(s)	262, 3077
AoxI	GGCC	3 cut(s)	282, 740, 2143
ApaLI	GTGCAC	1 cut(s)	1588
ApeKI	GCWGC	5 cut(s)	638, 1556, 1991, 2170, 2198
ApoI	RAATTY	6 cut(s)	86, 581, 1066, 1283, 2714, 3213
Asp700I	GAANNNNTTC	4 cut(s)	2963, 3276, 3335, 3617
Asp718I	GGTACC	1 cut(s)	2439
AspA2I	CCTAGG	1 cut(s)	737
AspS9I	GGNCC	3 cut(s)	193, 283, 1235
AsuC2I	CCSGG	7 cut(s)	104, 209, 263, 264, 1251, 1909, 2602
AsuHPI	GGTGA	9 cut(s)	25, 472, 547, 782, 853, 1289, 1352, 1382, 3205
AsuII	TTCGAA	2 cut(s)	578, 2879
AsuNHI	GCTAGC	1 cut(s)	2381
AvaI	CYCGRG	2 cut(s)	262, 3077
AvaII	GGWCC	2 cut(s)	193, 1235
AvrII	CCTAGG	1 cut(s)	737
AxyI	CCTNAGG	2 cut(s)	1986, 2673
BaeGI	GKGCMC	4 cut(s)	263, 323, 1592, 1865
BaeI	ACNNNNGTAYC	2 cut(s)	2431, 2464
BalI	TGGCCA	1 cut(s)	2145
BamHI	GGATCC	2 cut(s)	424, 2554
BanI	GGYRCC	3 cut(s)	772, 1253, 2439
BanII	GRGCYC	1 cut(s)	2923
BarI	GAAGNNNNNNTAC	2 cut(s)	171, 203
BauI	CACGAG	1 cut(s)	3546
BbsI	GAAGAC	1 cut(s)	1371
Bbv12I	GWGCWC	5 cut(s)	1592, 1902, 2687, 2923, 3578
BbvCI	CCTCAGC	1 cut(s)	1457
BbvI	GCAGC	5 cut(s)	650, 1543, 2003, 2182, 2210
BceAI	ACGGC	1 cut(s)	1519
BciT130I	CCWGG	2 cut(s)	37, 919
BclI	TGATCA	3 cut(s)	2211, 2776, 3135
BcnI	CCSGG	7 cut(s)	104, 209, 263, 264, 1251, 1909, 2602
BcoDI	GTCTC	6 cut(s)	637, 738, 1428, 1968, 3080, 3281
BfaI	CTAG	6 cut(s)	26, 738, 1361, 2382, 2832, 3455
BfmI	CTRYAG	5 cut(s)	636, 2409, 2559, 3129, 3282
BfoI	RGCGCY	1 cut(s)	3014
BglII	AGATCT	2 cut(s)	1259, 1608
BlnI	CCTAGG	1 cut(s)	737
BlpI	GCTNAGC	2 cut(s)	203, 2014
Bme1390I	CCNGG	9 cut(s)	37, 104, 209, 263, 264, 919, 1251, 1909, 2602
Bme18I	GGWCC	2 cut(s)	193, 1235
BmeT110I	CYCGRG	2 cut(s)	262, 3077
BmgBI	CACGTC	1 cut(s)	2994
BmgT120I	GGNCC	3 cut(s)	193, 283, 1235
BmiI	GGNNCC	7 cut(s)	195, 248, 426, 774, 1255, 2441, 2556
BmrFI	CCNGG	9 cut(s)	37, 104, 209, 263, 264, 919, 1251, 1909, 2602
BmrI	ACTGGG	1 cut(s)	3616
BmsI	GCATC	7 cut(s)	987, 1276, 2185, 3154, 3172, 3476, 3564
BmtI	GCTAGC	1 cut(s)	2385
BmuI	ACTGGG	1 cut(s)	3616
BoxI	GACNNNNGTC	1 cut(s)	629
BpiI	GAAGAC	1 cut(s)	1371
BpmI	CTGGAG	1 cut(s)	2997
Bpu10I	CCTNAGC	2 cut(s)	1434, 1457
Bpu1102I	GCTNAGC	2 cut(s)	203, 2014
Bpu14I	TTCGAA	2 cut(s)	578, 2879
BpuEI	CTTGAG	3 cut(s)	1022, 1611, 2422
BpuMI	CCSGG	7 cut(s)	104, 209, 263, 264, 1251, 1909, 2602
Bsa29I	ATCGAT	1 cut(s)	3203
BsaBI	GATNNNNATC	1 cut(s)	2775
BsaHI	GRCGYC	1 cut(s)	1354
BsaWI	WCCGGW	1 cut(s)	161
Bse118I	RCCGGY	2 cut(s)	485, 2690
Bse1I	ACTGG	4 cut(s)	126, 195, 1099, 3611
Bse21I	CCTNAGG	2 cut(s)	1986, 2673
Bse3DI	GCAATG	4 cut(s)	829, 1863, 2039, 3508
Bse8I	GATNNNNATC	1 cut(s)	2775
BseBI	CCWGG	2 cut(s)	37, 919
BseCI	ATCGAT	1 cut(s)	3203
BseJI	GATNNNNATC	1 cut(s)	2775
BseMI	GCAATG	4 cut(s)	829, 1863, 2039, 3508
BseMII	CTCAG	5 cut(s)	1448, 1769, 1984, 2000, 2664
BseNI	ACTGG	4 cut(s)	126, 195, 1099, 3611
BsePI	GCGCGC	2 cut(s)	872, 2028
BseRI	GAGGAG	2 cut(s)	1553, 3578
BseSI	GKGCMC	4 cut(s)	263, 323, 1592, 1865
BseXI	GCAGC	5 cut(s)	650, 1543, 2003, 2182, 2210
BseYI	CCCAGC	2 cut(s)	904, 2678
BsgI	GTGCAG	2 cut(s)	1049, 3539
Bsh1236I	CGCG	8 cut(s)	199, 874, 883, 885, 1062, 2030, 2097, 2301
Bsh1285I	CGRYCG	1 cut(s)	574
BshFI	GGCC	3 cut(s)	284, 742, 2145
BshNI	GGYRCC	3 cut(s)	772, 1253, 2439
BshVI	ATCGAT	1 cut(s)	3203
BsiEI	CGRYCG	1 cut(s)	574
BsiHKAI	GWGCWC	5 cut(s)	1592, 1902, 2687, 2923, 3578
BsiHKCI	CYCGRG	2 cut(s)	262, 3077
BsiWI	CGTACG	1 cut(s)	1759
BslFI	GGGAC	7 cut(s)	85, 544, 637, 1133, 1511, 2203, 3481
BsmAI	GTCTC	6 cut(s)	637, 738, 1428, 1968, 3080, 3281
BsmFI	GGGAC	7 cut(s)	85, 544, 637, 1133, 1511, 2203, 3481
BsmI	GAATGC	2 cut(s)	561, 874
BsnI	GGCC	3 cut(s)	284, 742, 2145
BsoBI	CYCGRG	2 cut(s)	262, 3077
Bsp119I	TTCGAA	2 cut(s)	578, 2879
Bsp1286I	GDGCHC	8 cut(s)	263, 323, 1592, 1865, 1902, 2687, 2923, 3578
Bsp1720I	GCTNAGC	2 cut(s)	203, 2014
Bsp19I	CCATGG	1 cut(s)	2047
BspANI	GGCC	3 cut(s)	284, 742, 2145
BspCNI	CTCAG	5 cut(s)	1449, 1770, 1983, 1999, 2665
BspDI	ATCGAT	1 cut(s)	3203
BspFNI	CGCG	8 cut(s)	199, 874, 883, 885, 1062, 2030, 2097, 2301
BspHI	TCATGA	1 cut(s)	3381
BspLI	GGNNCC	7 cut(s)	195, 248, 426, 774, 1255, 2441, 2556
BspMAI	CTGCAG	3 cut(s)	640, 2563, 3286
BspOI	GCTAGC	1 cut(s)	2385
BspPI	GGATC	8 cut(s)	419, 432, 1313, 2245, 2481, 2549, 2562, 3542
BspQI	GCTCTTC	1 cut(s)	1971
BspT104I	TTCGAA	2 cut(s)	578, 2879
BspT107I	GGYRCC	3 cut(s)	772, 1253, 2439
BsrDI	GCAATG	4 cut(s)	829, 1863, 2039, 3508
BsrFI	RCCGGY	2 cut(s)	485, 2690
BsrI	ACTGG	4 cut(s)	126, 195, 1099, 3611
BssAI	RCCGGY	2 cut(s)	485, 2690
BssHII	GCGCGC	2 cut(s)	872, 2028
BssNAI	GTATAC	2 cut(s)	2522, 3098
BssNI	GRCGYC	1 cut(s)	1354
BssSI	CACGAG	1 cut(s)	3546
BssT1I	CCWWGG	4 cut(s)	737, 1290, 1445, 2047
Bst1107I	GTATAC	2 cut(s)	2522, 3098
Bst2BI	CACGAG	1 cut(s)	3546
Bst2UI	CCWGG	2 cut(s)	37, 919
Bst6I	CTCTTC	2 cut(s)	1971, 2094
BstACI	GRCGYC	1 cut(s)	1354
BstAPI	GCANNNNNTGC	4 cut(s)	482, 1556, 1997, 2010
BstBI	TTCGAA	2 cut(s)	578, 2879
BstDSI	CCRYGG	2 cut(s)	197, 2047
BstEII	GGTNACC	1 cut(s)	2042
BstFNI	CGCG	8 cut(s)	199, 874, 883, 885, 1062, 2030, 2097, 2301
BstH2I	RGCGCY	1 cut(s)	3014
BstMAI	GTCTC	6 cut(s)	637, 738, 1428, 1968, 3080, 3281
BstMCI	CGRYCG	1 cut(s)	574
BstNI	CCWGG	2 cut(s)	37, 919
BstNSI	RCATGY	2 cut(s)	1197, 2631
BstPAI	GACNNNNGTC	1 cut(s)	629
BstPI	GGTNACC	1 cut(s)	2042
BstSCI	CCNGG	9 cut(s)	35, 102, 207, 261, 262, 917, 1249, 1907, 2600
BstSFI	CTRYAG	5 cut(s)	636, 2409, 2559, 3129, 3282
BstSLI	GKGCMC	4 cut(s)	263, 323, 1592, 1865
BstUI	CGCG	8 cut(s)	199, 874, 883, 885, 1062, 2030, 2097, 2301
BstV1I	GCAGC	5 cut(s)	650, 1543, 2003, 2182, 2210
BstV2I	GAAGAC	1 cut(s)	1371
BstX2I	RGATCY	6 cut(s)	424, 1259, 1608, 2237, 2473, 2554
BstXI	CCANNNNNNTGG	4 cut(s)	769, 1159, 2467, 2975
BstYI	RGATCY	6 cut(s)	424, 1259, 1608, 2237, 2473, 2554
BstZ17I	GTATAC	2 cut(s)	2522, 3098
Bsu15I	ATCGAT	1 cut(s)	3203
Bsu36I	CCTNAGG	2 cut(s)	1986, 2673
BsuRI	GGCC	3 cut(s)	284, 742, 2145
BsuTUI	ATCGAT	1 cut(s)	3203
BtgI	CCRYGG	2 cut(s)	197, 2047
BtgZI	GCGATG	1 cut(s)	1521
BtrI	CACGTC	1 cut(s)	2994
BtsI	GCAGTG	3 cut(s)	1037, 2568, 3003
BtsIMutI	CAGTG	6 cut(s)	1037, 1900, 2505, 2568, 3003, 3129
CaiI	CAGNNNCTG	3 cut(s)	1556, 2192, 3290
CciI	TCATGA	1 cut(s)	3381
Cfr10I	RCCGGY	2 cut(s)	485, 2690
Cfr13I	GGNCC	3 cut(s)	193, 283, 1235
Cfr42I	CCGCGG	1 cut(s)	200
Cfr9I	CCCGGG	1 cut(s)	262
ClaI	ATCGAT	1 cut(s)	3203
CseI	GACGC	4 cut(s)	1362, 1462, 2953, 3535
Csp6I	GTAC	7 cut(s)	69, 471, 1177, 1246, 1760, 2440, 3424
CspCI	CAANNNNNGTGG	2 cut(s)	2614, 2649
CviQI	GTAC	7 cut(s)	69, 471, 1177, 1246, 1760, 2440, 3424
EaeI	YGGCCR	1 cut(s)	2143
Eam1104I	CTCTTC	2 cut(s)	1971, 2094
EarI	CTCTTC	2 cut(s)	1971, 2094
EciI	GGCGGA	3 cut(s)	140, 511, 3073
Ecl136II	GAGCTC	1 cut(s)	2921
Eco130I	CCWWGG	4 cut(s)	737, 1290, 1445, 2047
Eco24I	GRGCYC	1 cut(s)	2923
Eco47I	GGWCC	2 cut(s)	193, 1235
Eco53kI	GAGCTC	1 cut(s)	2921
Eco57I	CTGAAG	6 cut(s)	359, 395, 780, 929, 2510, 3251
Eco81I	CCTNAGG	2 cut(s)	1986, 2673
Eco88I	CYCGRG	2 cut(s)	262, 3077
Eco91I	GGTNACC	1 cut(s)	2042
EcoICRI	GAGCTC	1 cut(s)	2921
EcoO65I	GGTNACC	1 cut(s)	2042
EcoRI	GAATTC	1 cut(s)	1283
EcoRII	CCWGG	2 cut(s)	35, 917
EcoT14I	CCWWGG	4 cut(s)	737, 1290, 1445, 2047
EcoT38I	GRGCYC	1 cut(s)	2923
ErhI	CCWWGG	4 cut(s)	737, 1290, 1445, 2047
FaqI	GGGAC	7 cut(s)	85, 544, 637, 1133, 1511, 2203, 3481
FauI	CCCGC	7 cut(s)	204, 381, 509, 634, 1055, 1536, 2812
FbaI	TGATCA	3 cut(s)	2211, 2776, 3135
FblI	GTMKAC	3 cut(s)	300, 2521, 3097
FriOI	GRGCYC	1 cut(s)	2923
FspAI	RTGCGCAY	1 cut(s)	3168
FspBI	CTAG	6 cut(s)	26, 738, 1361, 2382, 2832, 3455
FspI	TGCGCA	1 cut(s)	3168
GsaI	CCCAGC	2 cut(s)	908, 2682
GsuI	CTGGAG	1 cut(s)	2997
HaeII	RGCGCY	1 cut(s)	3014
HaeIII	GGCC	3 cut(s)	284, 742, 2145
HgaI	GACGC	4 cut(s)	1362, 1462, 2953, 3535
Hin1I	GRCGYC	1 cut(s)	1354
HincII	GTYRAC	1 cut(s)	2983
HindII	GTYRAC	1 cut(s)	2983
HindIII	AAGCTT	1 cut(s)	1168
HpaI	GTTAAC	1 cut(s)	2983
HphI	GGTGA	9 cut(s)	25, 472, 547, 782, 853, 1289, 1352, 1382, 3205
Hpy166II	GTNNAC	9 cut(s)	301, 526, 1590, 1731, 1774, 2522, 2983, 3098, 3481
Hpy188I	TCNGA	8 cut(s)	1638, 1791, 1973, 2194, 2656, 2918, 3238, 3513
Hpy8I	GTNNAC	9 cut(s)	301, 526, 1590, 1731, 1774, 2522, 2983, 3098, 3481
Hpy99I	CGWCG	2 cut(s)	311, 2947
HpyCH4IV	ACGT	7 cut(s)	306, 333, 1081, 1175, 2869, 2993, 3507
HpySE526I	ACGT	7 cut(s)	306, 333, 1081, 1175, 2869, 2993, 3507
Hsp92I	GRCGYC	1 cut(s)	1354
KpnI	GGTACC	1 cut(s)	2443
Ksp22I	TGATCA	3 cut(s)	2211, 2776, 3135
KspAI	GTTAAC	1 cut(s)	2983
KspI	CCGCGG	1 cut(s)	200
LguI	GCTCTTC	1 cut(s)	1971
LmnI	GCTCC	4 cut(s)	2178, 2590, 2918, 3297
Lsp1109I	GCAGC	5 cut(s)	650, 1543, 2003, 2182, 2210
LweI	GCATC	7 cut(s)	987, 1276, 2185, 3154, 3172, 3476, 3564
MaeI	CTAG	6 cut(s)	26, 738, 1361, 2382, 2832, 3455
MaeII	ACGT	7 cut(s)	306, 333, 1081, 1175, 2869, 2993, 3507
MaeIII	GTNAC	9 cut(s)	955, 1189, 1340, 1370, 1801, 1889, 2042, 2824, 2870
MfeI	CAATTG	3 cut(s)	1742, 2058, 2904
MflI	RGATCY	6 cut(s)	424, 1259, 1608, 2237, 2473, 2554
MhlI	GDGCHC	8 cut(s)	263, 323, 1592, 1865, 1902, 2687, 2923, 3578
MlsI	TGGCCA	1 cut(s)	2145
MluNI	TGGCCA	1 cut(s)	2145
MlyI	GAGTC	6 cut(s)	1155, 1323, 1791, 2413, 2662, 3558
MmeI	TCCRAC	6 cut(s)	146, 1123, 1330, 2200, 2571, 2820
Mox20I	TGGCCA	1 cut(s)	2145
MroXI	GAANNNNTTC	4 cut(s)	2963, 3276, 3335, 3617
MscI	TGGCCA	1 cut(s)	2145
MslI	CAYNNNNRTG	6 cut(s)	326, 770, 839, 1157, 1272, 1699
Msp20I	TGGCCA	1 cut(s)	2145
MspA1I	CMGCKG	3 cut(s)	199, 641, 1994
MspR9I	CCNGG	9 cut(s)	37, 104, 209, 263, 264, 919, 1251, 1909, 2602
MunI	CAATTG	3 cut(s)	1742, 2058, 2904
Mva1269I	GAATGC	2 cut(s)	561, 874
MvaI	CCWGG	2 cut(s)	37, 919
MvnI	CGCG	8 cut(s)	199, 874, 883, 885, 1062, 2030, 2097, 2301
NciI	CCSGG	7 cut(s)	104, 209, 263, 264, 1251, 1909, 2602
NcoI	CCATGG	1 cut(s)	2047
NheI	GCTAGC	1 cut(s)	2381
NlaIV	GGNNCC	7 cut(s)	195, 248, 426, 774, 1255, 2441, 2556
NmuCI	GTSAC	3 cut(s)	1340, 1370, 1801
NsbI	TGCGCA	1 cut(s)	3168
NspI	RCATGY	2 cut(s)	1197, 2631
NspV	TTCGAA	2 cut(s)	578, 2879
OliI	CACNNNNGTG	1 cut(s)	1699
PagI	TCATGA	1 cut(s)	3381
PasI	CCCWGGG	1 cut(s)	918
PauI	GCGCGC	2 cut(s)	872, 2028
PciI	ACATGT	2 cut(s)	1193, 2627
PciSI	GCTCTTC	1 cut(s)	1971
PcsI	WCGNNNNNNNCGW	1 cut(s)	294
PctI	GAATGC	2 cut(s)	561, 874
PdmI	GAANNNNTTC	4 cut(s)	2963, 3276, 3335, 3617
Pfl23II	CGTACG	1 cut(s)	1759
PflMI	CCANNNNNTGG	1 cut(s)	2431
PleI	GAGTC	6 cut(s)	1154, 1323, 1790, 2412, 2662, 3557
PpsI	GAGTC	6 cut(s)	1154, 1323, 1790, 2412, 2662, 3557
PscI	ACATGT	2 cut(s)	1193, 2627
PshAI	GACNNNNGTC	1 cut(s)	629
PsiI	TTATAA	1 cut(s)	1655
Psp124BI	GAGCTC	1 cut(s)	2923
Psp6I	CCWGG	2 cut(s)	35, 917
PspEI	GGTNACC	1 cut(s)	2042
PspFI	CCCAGC	2 cut(s)	904, 2678
PspGI	CCWGG	2 cut(s)	35, 917
PspLI	CGTACG	1 cut(s)	1759
PspN4I	GGNNCC	7 cut(s)	195, 248, 426, 774, 1255, 2441, 2556
PspPI	GGNCC	3 cut(s)	193, 283, 1235
PsrI	GAACNNNNNNTAC	2 cut(s)	3416, 3448
PstI	CTGCAG	3 cut(s)	640, 2563, 3286
PstNI	CAGNNNCTG	3 cut(s)	1556, 2192, 3290
PsuI	RGATCY	6 cut(s)	424, 1259, 1608, 2237, 2473, 2554
PteI	GCGCGC	2 cut(s)	872, 2028
PvuII	CAGCTG	1 cut(s)	1994
RsaI	GTAC	7 cut(s)	70, 472, 1178, 1247, 1761, 2441, 3425
RsaNI	GTAC	7 cut(s)	69, 471, 1177, 1246, 1760, 2440, 3424
RseI	CAYNNNNRTG	6 cut(s)	326, 770, 839, 1157, 1272, 1699
SacI	GAGCTC	1 cut(s)	2923
SacII	CCGCGG	1 cut(s)	200
SapI	GCTCTTC	1 cut(s)	1971
Sau96I	GGNCC	3 cut(s)	193, 283, 1235
SchI	GAGTC	6 cut(s)	1155, 1323, 1791, 2413, 2662, 3558
ScrFI	CCNGG	9 cut(s)	37, 104, 209, 263, 264, 919, 1251, 1909, 2602
SduI	GDGCHC	8 cut(s)	263, 323, 1592, 1865, 1902, 2687, 2923, 3578
SfaNI	GCATC	7 cut(s)	987, 1276, 2185, 3154, 3172, 3476, 3564
SfcI	CTRYAG	5 cut(s)	636, 2409, 2559, 3129, 3282
Sfr303I	CCGCGG	1 cut(s)	200
SfuI	TTCGAA	2 cut(s)	578, 2879
SgrBI	CCGCGG	1 cut(s)	200
SinI	GGWCC	2 cut(s)	193, 1235
SmaI	CCCGGG	1 cut(s)	264
SmiMI	CAYNNNNRTG	6 cut(s)	326, 770, 839, 1157, 1272, 1699
SmlI	CTYRAG	3 cut(s)	1037, 1626, 2401
SmoI	CTYRAG	3 cut(s)	1037, 1626, 2401
SspI	AATATT	2 cut(s)	1204, 2815
SspMI	CTAG	6 cut(s)	26, 738, 1361, 2382, 2832, 3455
SstI	GAGCTC	1 cut(s)	2923
StyD4I	CCNGG	9 cut(s)	35, 102, 207, 261, 262, 917, 1249, 1907, 2600
StyI	CCWWGG	4 cut(s)	737, 1290, 1445, 2047
TaiI	ACGT	7 cut(s)	309, 336, 1084, 1178, 2872, 2996, 3510
TaqII	GACCGA	2 cut(s)	1847, 2337
TatI	WGTACW	2 cut(s)	470, 3423
TauI	GCSGC	7 cut(s)	186, 202, 778, 2366, 2400, 3011, 3545
TscAI	CASTG	6 cut(s)	1037, 1900, 2505, 2568, 3010, 3129
TseFI	GTSAC	3 cut(s)	1340, 1370, 1801
TseI	GCWGC	5 cut(s)	638, 1556, 1991, 2170, 2198
Tsp45I	GTSAC	3 cut(s)	1340, 1370, 1801
TspGWI	ACGGA	5 cut(s)	515, 1002, 1473, 2577, 3498
TspMI	CCCGGG	1 cut(s)	262
TspRI	CASTG	6 cut(s)	1037, 1900, 2505, 2568, 3010, 3129
Van91I	CCANNNNNTGG	1 cut(s)	2431
VneI	GTGCAC	1 cut(s)	1588
VpaK11BI	GGWCC	2 cut(s)	193, 1235
XapI	RAATTY	6 cut(s)	86, 581, 1066, 1283, 2714, 3213
XbaI	TCTAGA	3 cut(s)	1360, 2831, 3454
XceI	RCATGY	2 cut(s)	1197, 2631
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2285
XmaI	CCCGGG	1 cut(s)	262
XmaJI	CCTAGG	1 cut(s)	737
XmiI	GTMKAC	3 cut(s)	300, 2521, 3097
XmnI	GAANNNNTTC	4 cut(s)	2963, 3276, 3335, 3617
XspI	CTAG	6 cut(s)	26, 738, 1361, 2382, 2832, 3455

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.