pycom09g11150

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002018
Physical Location & Seq
Reverse (-)
9279443 .. 9281257
1815 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 810 bp
ATGTACTCAGTAAATAATACGAATATTTTCGCTTCCTTCTGCATTCACAGGCAAACTCTCTCTCCCTCCCTCTCCCTCCCTCTCCCTCTCTCTCTCGCTCTCTCTCTCTCTCTCTCTGTGCAAACGCCGATGGCTGAGAGACCCAGATTGCAAATCCGTGACCTTCTATTTCGTATAGATTATTCCGCCTACAGCTTTACGACGAGTTCGAGCTCGCTGAAGATCGGCGAGACTCTCTGTGCGTTGTGCAGACCACTGATTGGCCTGGTCGAGGCTTTCATCTTCAGCTTAGCTGGTTGCTTCGATTGCCACTGTCAACCTCACCGCCTTTCCTTCACCCACGACGACGTCGTTCGACTCGCCGCCACCTCTCCATTTAGTGTGAATGAAGTGGAAGCATTGCGCGAACTGTTTAACAAGTTAAGTAGCTCCATTCTCGACGATGGATTACTTCACAAGGAGGAGCTGAGGTTGGCGCTTTTCAAAACTCTAGCTGGTGAAAATCTTTTTCTTGATAGGGTGTTTGATGTTTTTGACGAAAAGAAAGATGGAGTGATTGAATTTGAGGAGTTTGTCCACGCACTAAGTGTGTTTCATCCGTATGCTTCTCTTGAGGCCAAGATTGATTTCGCATTTAGGTTGTATGATTTGAGGCAAACCGGATACATCGGGCGGGAAGAAGTCCGGCAAATGGTAGTTGCTACTTTGTTGGAATCCGGCATTCAGATGCCGGATGAAACTCTGGAAGCTATCGTAGATAAGGCAAGAATTGTGGATGTTTTAAATTTGTGTTCTTTACCTTTATTCTGA

Protein Analysis

270

Amino Acids

30.31

Weight (kDa)

5.1

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 171 - 196 5.7e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
AccB7I CCANNNNNTGG 1 cut(s) 260
AccII CGCG 1 cut(s) 405
AciI CCGC 4 cut(s) 186, 325, 363, 673
AcsI RAATTY 2 cut(s) 560, 784
AcuI CTGAAG 2 cut(s) 239, 268
AcyI GRCGYC 1 cut(s) 348
AdeI CACNNNGTG 1 cut(s) 587
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 3 cut(s) 260, 271, 691
AgsI TTSAA 2 cut(s) 484, 560
AjnI CCWGG 1 cut(s) 264
AjuI GAANNNNNNNTTGG 2 cut(s) 611, 643
AluBI AGCT 8 cut(s) 195, 213, 288, 293, 429, 466, 494, 749
AluI AGCT 8 cut(s) 195, 213, 288, 293, 429, 466, 494, 749
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 2 cut(s) 133, 224
AoxI GGCC 2 cut(s) 262, 615
ApoI RAATTY 2 cut(s) 560, 784
Asp700I GAANNNNTTC 1 cut(s) 26
AspLEI GCGC 2 cut(s) 405, 478
AsuHPI GGTGA 3 cut(s) 314, 328, 509
BanII GRGCYC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 215
BbvCI CCTCAGC 1 cut(s) 467
BccI CCATC 3 cut(s) 124, 437, 542
BciT130I CCWGG 1 cut(s) 266
BciVI GTATCC 1 cut(s) 656
BcoDI GTCTC 2 cut(s) 133, 224
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 190
BfoI RGCGCY 1 cut(s) 479
BfuI GTATCC 1 cut(s) 656
BisI GCNGC 1 cut(s) 363
BlpI GCTNAGC 1 cut(s) 289
BlsI GCNGC 1 cut(s) 364
Bme1390I CCNGG 1 cut(s) 266
BmrFI CCNGG 1 cut(s) 266
BmsI GCATC 1 cut(s) 717
Bpu10I CCTNAGC 1 cut(s) 467
Bpu1102I GCTNAGC 1 cut(s) 289
BpuEI CTTGAG 1 cut(s) 632
BsaHI GRCGYC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 133
BsaWI WCCGGW 1 cut(s) 659
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 455, 485
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 271, 691
Bse3DI GCAATG 1 cut(s) 398
BseBI CCWGG 1 cut(s) 266
BseGI GGATG 3 cut(s) 595, 739, 781
BseLI CCNNNNNNNGG 3 cut(s) 260, 271, 691
BseMI GCAATG 1 cut(s) 398
BseMII CTCAG 3 cut(s) 21, 126, 458
BseRI GAGGAG 2 cut(s) 476, 581
BsgI GTGCAG 1 cut(s) 268
Bsh1236I CGCG 1 cut(s) 405
BshFI GGCC 2 cut(s) 264, 617
BsiHKAI GWGCWC 1 cut(s) 215
BsiSI CCGG 4 cut(s) 660, 685, 717, 731
BslI CCNNNNNNNGG 3 cut(s) 260, 271, 691
BsmAI GTCTC 2 cut(s) 133, 224
BsmI GAATGC 2 cut(s) 42, 720
BsnI GGCC 2 cut(s) 264, 617
Bso31I GGTCTC 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 1 cut(s) 222
Bsp1720I GCTNAGC 1 cut(s) 289
BspACI CCGC 4 cut(s) 186, 325, 363, 673
BspANI GGCC 2 cut(s) 264, 617
BspCNI CTCAG 3 cut(s) 20, 127, 459
BspFNI CGCG 1 cut(s) 405
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 398
BssMI GATC 1 cut(s) 222
BssNI GRCGYC 1 cut(s) 348
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 2 cut(s) 314, 411
BstACI GRCGYC 1 cut(s) 348
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 5 cut(s) 7, 135, 289, 467, 584
BstENI CCTNNNNNAGG 1 cut(s) 269
BstF5I GGATG 3 cut(s) 595, 739, 781
BstFNI CGCG 1 cut(s) 405
BstH2I RGCGCY 1 cut(s) 479
BstHHI GCGC 2 cut(s) 405, 478
BstKTI GATC 1 cut(s) 225
BstMAI GTCTC 2 cut(s) 133, 224
BstMBI GATC 1 cut(s) 222
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 1 cut(s) 266
BstSCI CCNGG 1 cut(s) 264
BstSFI CTRYAG 1 cut(s) 190
BstUI CGCG 1 cut(s) 405
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 2 cut(s) 264, 617
BtsCI GGATG 3 cut(s) 595, 739, 781
BtsIMutI CAGTG 2 cut(s) 254, 310
Cac8I GCNNGC 1 cut(s) 215
CfoI GCGC 2 cut(s) 405, 478
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 2 cut(s) 753, 788
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 5 cut(s) 7, 135, 289, 467, 584
DpnI GATC 1 cut(s) 224
DpnII GATC 1 cut(s) 222
DraI TTTAAA 1 cut(s) 783
DraIII CACNNNGTG 1 cut(s) 587
EciI GGCGGA 1 cut(s) 175
Ecl136II GAGCTC 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 215
Eco31I GGTCTC 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 213
Eco57I CTGAAG 2 cut(s) 239, 268
EcoICRI GAGCTC 1 cut(s) 213
EcoNI CCTNNNNNAGG 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 215
FaiI YATR 3 cut(s) 176, 603, 645
FauI CCCGC 1 cut(s) 666
Fnu4HI GCNGC 1 cut(s) 363
FokI GGATG 3 cut(s) 582, 746, 788
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 363
FspBI CTAG 1 cut(s) 491
GlaI GCGC 2 cut(s) 404, 477
GluI GCNGC 1 cut(s) 363
HaeII RGCGCY 1 cut(s) 479
HaeIII GGCC 2 cut(s) 264, 617
HapII CCGG 4 cut(s) 660, 685, 717, 731
HhaI GCGC 2 cut(s) 405, 478
Hin1I GRCGYC 1 cut(s) 348
Hin6I GCGC 2 cut(s) 403, 476
HinP1I GCGC 2 cut(s) 403, 476
HincII GTYRAC 1 cut(s) 317
HindII GTYRAC 1 cut(s) 317
HinfI GANTC 3 cut(s) 232, 357, 713
HpaII CCGG 4 cut(s) 660, 685, 717, 731
HphI GGTGA 3 cut(s) 314, 328, 509
Hpy166II GTNNAC 2 cut(s) 317, 577
Hpy188I TCNGA 2 cut(s) 726, 809
Hpy188III TCNNGA 4 cut(s) 437, 512, 611, 743
Hpy8I GTNNAC 2 cut(s) 317, 577
Hpy99I CGWCG 5 cut(s) 205, 347, 350, 353, 443
HpyAV CCTTC 3 cut(s) 46, 173, 343
HpyCH4III ACNGT 2 cut(s) 314, 411
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 4 cut(s) 42, 121, 151, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 5 cut(s) 7, 135, 289, 467, 584
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 1 cut(s) 348
HspAI GCGC 2 cut(s) 403, 476
Kzo9I GATC 1 cut(s) 222
LmnI GCTCC 2 cut(s) 434, 463
LweI GCATC 1 cut(s) 717
MaeI CTAG 1 cut(s) 491
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 1 cut(s) 158
MalI GATC 1 cut(s) 224
MboI GATC 1 cut(s) 222
MboII GAAGA 3 cut(s) 232, 274, 689
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 3 cut(s) 560, 768, 784
MlyI GAGTC 2 cut(s) 226, 351
MmeI TCCRAC 1 cut(s) 690
MroXI GAANNNNTTC 1 cut(s) 26
MseI TTAA 3 cut(s) 414, 422, 782
MslI CAYNNNNRTG 2 cut(s) 600, 725
MspI CCGG 4 cut(s) 660, 685, 717, 731
MspR9I CCNGG 1 cut(s) 266
Mva1269I GAATGC 2 cut(s) 42, 720
MvaI CCWGG 1 cut(s) 266
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 1 cut(s) 306
NdeII GATC 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 2 cut(s) 206, 348
PctI GAATGC 2 cut(s) 42, 720
PdmI GAANNNNTTC 1 cut(s) 26
PfeI GAWTC 1 cut(s) 713
PflFI GACNNNGTC 1 cut(s) 347
PflMI CCANNNNNTGG 1 cut(s) 260
PkrI GCNGC 1 cut(s) 364
PleI GAGTC 2 cut(s) 226, 351
PpsI GAGTC 2 cut(s) 226, 351
Psp124BI GAGCTC 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 264
PspGI CCWGG 1 cut(s) 264
PsyI GACNNNGTC 1 cut(s) 347
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 600, 725
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 3 cut(s) 414, 422, 782
SatI GCNGC 1 cut(s) 363
Sau3AI GATC 1 cut(s) 222
SchI GAGTC 2 cut(s) 226, 351
ScrFI CCNGG 1 cut(s) 266
SduI GDGCHC 1 cut(s) 215
SfaNI GCATC 1 cut(s) 717
SfcI CTRYAG 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 600, 725
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 3 cut(s) 560, 768, 784
SsiI CCGC 4 cut(s) 186, 325, 363, 673
SspI AATATT 1 cut(s) 25
SspMI CTAG 1 cut(s) 491
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 314, 411
TaiI ACGT 1 cut(s) 351
TaqI TCGA 5 cut(s) 209, 270, 303, 355, 438
TasI AATT 3 cut(s) 560, 768, 784
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 1 cut(s) 713
Tru1I TTAA 3 cut(s) 414, 422, 782
Tru9I TTAA 3 cut(s) 414, 422, 782
TscAI CASTG 2 cut(s) 261, 317
TseFI GTSAC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 4 cut(s) 268, 402, 584, 750
TspGWI ACGGA 2 cut(s) 146, 588
TspRI CASTG 2 cut(s) 261, 317
Tth111I GACNNNGTC 1 cut(s) 347
Van91I CCANNNNNTGG 1 cut(s) 260
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 2 cut(s) 560, 784
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 1 cut(s) 491
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.