pycom11g00690

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006472
Physical Location & Seq
Reverse (-)
517512 .. 520598
3087 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 840 bp
ATGAAATGTAACCCATGGTTTGGTCATTATCCATTGTCTGATTCTTCTGCCACCAACCAACCTGGACTTCAAGAAAAACCCATGATTTCCCATAAATACCACACCAACCTCAAAACAAATCCAAACCGTCCAAGCCTCTCAAATCCAACGGTACAGGCTCCTCTAAACCCCTCACGTCCTCAATATATACACCCAAAAATCTTTCCCGGAAAGTCCAACCTGCAAAGGATACTCAAGAACCCGAAAAGCACAGAACTTTGTCAAAACCCACACACACTCTCTATAGCACCCGACCCGAATATGGCACAGAACCCGAAACCCCATCTGGGTCTGATCCCGATCCTCCTCTTCTCTCTCTCCGTCCTCCCAAGTCTCTCTCTTTCTCTCTCTAACTCCCCACCCACAGTCTTCGACATTTTACCCGAATACGGCCTCCCCCGCGGCCTCTTACCGTCGTCAGTCTCCAACCACACGCTCTCCGACGACGGCCGATTCGTCGTCGTTTTGCCCAAGACCTGCTACATTCAGTTCGATTACTTGGTCTACTACGAAAAGACCATAACGGGCAAGCTCACATACGGGGCGATCACGGACTTGAAGGGGATTCAGGTCCAGAGGTTCTTGTTCTGGTTCGACGTCGACGAGATCAGGGTCGATTTGCCGCCCTCGGATAGCATTTACTTCACTGTTGGGATCATCAACAAGAAGCTCGACGTCGATCAATTTGAAAATGTCCGGTCTTGCCGTGACGGGTTATCGGGTTCTTGCGTCGGGTTGTTCAAACGGGGCATTCAGCTTCCAACCCCGGTGGAAGAAATTGAGATGCTAATTACCGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

280

Amino Acids

31.26

Weight (kDa)

8.21

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 138 - 243 6.2e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 639, 717
Acc36I ACCTGC 2 cut(s) 228, 524
AccB7I CCANNNNNTGG 1 cut(s) 20
AccI GTMKAC 2 cut(s) 543, 639
AccII CGCG 1 cut(s) 441
AciI CCGC 3 cut(s) 439, 441, 662
AclWI GGATC 3 cut(s) 328, 334, 701
AcoI YGGCCR 1 cut(s) 487
AcyI GRCGYC 2 cut(s) 636, 714
AfaI GTAC 1 cut(s) 153
AfiI CCNNNNNNNGG 4 cut(s) 20, 301, 326, 428
AgsI TTSAA 4 cut(s) 71, 598, 728, 781
AjiI CACGTC 1 cut(s) 176
AjnI CCWGG 1 cut(s) 61
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AluBI AGCT 3 cut(s) 571, 709, 796
AluI AGCT 3 cut(s) 571, 709, 796
Alw26I GTCTC 2 cut(s) 377, 466
AlwI GGATC 3 cut(s) 328, 334, 701
AoxI GGCC 3 cut(s) 430, 442, 487
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
AspS9I GGNCC 1 cut(s) 610
AsuC2I CCSGG 2 cut(s) 207, 806
AvaII GGWCC 1 cut(s) 610
BbsI GAAGAC 1 cut(s) 400
BccI CCATC 1 cut(s) 330
BceAI ACGGC 3 cut(s) 445, 502, 729
BciT130I CCWGG 1 cut(s) 63
BciVI GTATCC 1 cut(s) 222
BcnI CCSGG 2 cut(s) 207, 806
BcoDI GTCTC 2 cut(s) 377, 466
BfmI CTRYAG 1 cut(s) 282
BfuAI ACCTGC 2 cut(s) 228, 524
BfuI GTATCC 1 cut(s) 222
BisI GCNGC 2 cut(s) 442, 662
BlsI GCNGC 2 cut(s) 443, 663
Bme1390I CCNGG 3 cut(s) 63, 207, 806
Bme18I GGWCC 1 cut(s) 610
BmgBI CACGTC 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 610
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 3 cut(s) 63, 207, 806
BmsI GCATC 1 cut(s) 813
BpiI GAAGAC 1 cut(s) 400
BpuEI CTTGAG 1 cut(s) 218
BpuMI CCSGG 2 cut(s) 207, 806
BsaBI GATNNNNATC 1 cut(s) 338
BsaHI GRCGYC 2 cut(s) 636, 714
BsaJI CCNNGG 4 cut(s) 14, 439, 666, 804
BsaWI WCCGGW 1 cut(s) 735
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 301, 326, 428
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 4 cut(s) 14, 439, 666, 804
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 4 cut(s) 20, 301, 326, 428
BseRI GAGGAG 2 cut(s) 150, 335
BseX3I CGGCCG 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 441
Bsh1285I CGRYCG 1 cut(s) 490
BshFI GGCC 3 cut(s) 432, 444, 489
BsiEI CGRYCG 1 cut(s) 490
BsiSI CCGG 3 cut(s) 207, 736, 806
BslI CCNNNNNNNGG 4 cut(s) 20, 301, 326, 428
BsmAI GTCTC 2 cut(s) 377, 466
BsmI GAATGC 1 cut(s) 789
BsnI GGCC 3 cut(s) 432, 444, 489
Bsp143I GATC 6 cut(s) 333, 339, 585, 645, 693, 718
Bsp19I CCATGG 1 cut(s) 14
BspACI CCGC 3 cut(s) 439, 441, 662
BspANI GGCC 3 cut(s) 432, 444, 489
BspFNI CGCG 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 159
BspMI ACCTGC 2 cut(s) 228, 524
BspPI GGATC 3 cut(s) 328, 334, 701
BssECI CCNNGG 4 cut(s) 14, 439, 666, 804
BssMI GATC 6 cut(s) 333, 339, 585, 645, 693, 718
BssNI GRCGYC 2 cut(s) 636, 714
BssT1I CCWWGG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 5 cut(s) 128, 151, 406, 453, 688
Bst6I CTCTTC 1 cut(s) 353
BstACI GRCGYC 2 cut(s) 636, 714
BstC8I GCNNGC 1 cut(s) 569
BstDSI CCRYGG 2 cut(s) 14, 439
BstFNI CGCG 1 cut(s) 441
BstKTI GATC 6 cut(s) 336, 342, 588, 648, 696, 721
BstMAI GTCTC 2 cut(s) 377, 466
BstMBI GATC 6 cut(s) 333, 339, 585, 645, 693, 718
BstMCI CGRYCG 1 cut(s) 490
BstMWI GCNNNNNNNGC 1 cut(s) 438
BstNI CCWGG 1 cut(s) 63
BstSCI CCNGG 3 cut(s) 61, 205, 804
BstSFI CTRYAG 1 cut(s) 282
BstUI CGCG 1 cut(s) 441
BstV2I GAAGAC 1 cut(s) 400
BstZI CGGCCG 1 cut(s) 487
BsuI GTATCC 1 cut(s) 222
BsuRI GGCC 3 cut(s) 432, 444, 489
BtgI CCRYGG 2 cut(s) 14, 439
BtrI CACGTC 1 cut(s) 176
BtsIMutI CAGTG 1 cut(s) 684
BveI ACCTGC 2 cut(s) 228, 524
Cac8I GCNNGC 1 cut(s) 569
Cfr13I GGNCC 1 cut(s) 610
Cfr42I CCGCGG 1 cut(s) 442
CseI GACGC 1 cut(s) 757
Csp6I GTAC 1 cut(s) 152
CspCI CAANNNNNGTGG 2 cut(s) 789, 824
CviAII CATG 2 cut(s) 15, 82
CviJI RGCY 8 cut(s) 135, 158, 432, 444, 489, 571, 709, 796
CviKI_1 RGCY 8 cut(s) 135, 158, 432, 444, 489, 571, 709, 796
CviQI GTAC 1 cut(s) 152
DpnI GATC 6 cut(s) 335, 341, 587, 647, 695, 720
DpnII GATC 6 cut(s) 333, 339, 585, 645, 693, 718
EaeI YGGCCR 1 cut(s) 487
EagI CGGCCG 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EclXI CGGCCG 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 610
Eco52I CGGCCG 1 cut(s) 487
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 14
ErhI CCWWGG 1 cut(s) 14
FaeI CATG 2 cut(s) 18, 85
FaiI YATR 9 cut(s) 16, 83, 93, 186, 188, 284, 302, 560, 577
FatI CATG 2 cut(s) 14, 81
FauI CCCGC 1 cut(s) 446
FblI GTMKAC 2 cut(s) 543, 639
Fnu4HI GCNGC 2 cut(s) 442, 662
Fsp4HI GCNGC 2 cut(s) 442, 662
GluI GCNGC 2 cut(s) 442, 662
HaeIII GGCC 3 cut(s) 432, 444, 489
HapII CCGG 3 cut(s) 207, 736, 806
HgaI GACGC 1 cut(s) 757
Hin1I GRCGYC 2 cut(s) 636, 714
Hin1II CATG 2 cut(s) 18, 85
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HinfI GANTC 3 cut(s) 41, 492, 604
HpaII CCGG 3 cut(s) 207, 736, 806
Hpy166II GTNNAC 2 cut(s) 544, 640
Hpy188I TCNGA 4 cut(s) 40, 333, 481, 670
Hpy188III TCNNGA 4 cut(s) 71, 235, 337, 613
Hpy8I GTNNAC 2 cut(s) 544, 640
HpyAV CCTTC 1 cut(s) 592
HpyCH4III ACNGT 5 cut(s) 128, 151, 406, 453, 688
HpyCH4IV ACGT 3 cut(s) 175, 636, 714
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 438
HpySE526I ACGT 3 cut(s) 175, 636, 714
Hsp92I GRCGYC 2 cut(s) 636, 714
Hsp92II CATG 2 cut(s) 18, 85
KspI CCGCGG 1 cut(s) 442
Kzo9I GATC 6 cut(s) 333, 339, 585, 645, 693, 718
LmnI GCTCC 1 cut(s) 163
LweI GCATC 1 cut(s) 813
MaeII ACGT 3 cut(s) 175, 636, 714
MaeIII GTNAC 2 cut(s) 8, 746
MalI GATC 6 cut(s) 335, 341, 587, 647, 695, 720
MboI GATC 6 cut(s) 333, 339, 585, 645, 693, 718
MboII GAAGA 4 cut(s) 36, 340, 400, 824
MluCI AATT 3 cut(s) 722, 816, 828
MmeI TCCRAC 5 cut(s) 170, 240, 489, 504, 824
MspA1I CMGCKG 1 cut(s) 441
MspI CCGG 3 cut(s) 207, 736, 806
MspR9I CCNGG 3 cut(s) 63, 207, 806
Mva1269I GAATGC 1 cut(s) 789
MvaI CCWGG 1 cut(s) 63
MvnI CGCG 1 cut(s) 441
MwoI GCNNNNNNNGC 1 cut(s) 438
NciI CCSGG 2 cut(s) 207, 806
NcoI CCATGG 1 cut(s) 14
NdeII GATC 6 cut(s) 333, 339, 585, 645, 693, 718
NlaIII CATG 2 cut(s) 18, 85
NlaIV GGNNCC 1 cut(s) 159
NmuCI GTSAC 1 cut(s) 746
PcsI WCGNNNNNNNCGW 2 cut(s) 492, 639
PctI GAATGC 1 cut(s) 789
PfeI GAWTC 3 cut(s) 41, 492, 604
PflMI CCANNNNNTGG 1 cut(s) 20
PfoI TCCNGGA 1 cut(s) 205
PkrI GCNGC 2 cut(s) 443, 663
Psp6I CCWGG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 61
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 1 cut(s) 610
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
SacII CCGCGG 1 cut(s) 442
SalI GTCGAC 1 cut(s) 638
SatI GCNGC 2 cut(s) 442, 662
Sau3AI GATC 6 cut(s) 333, 339, 585, 645, 693, 718
Sau96I GGNCC 1 cut(s) 610
ScrFI CCNGG 3 cut(s) 63, 207, 806
SfaNI GCATC 1 cut(s) 813
SfcI CTRYAG 1 cut(s) 282
Sfr303I CCGCGG 1 cut(s) 442
SgrBI CCGCGG 1 cut(s) 442
SgrDI CGTCGACG 1 cut(s) 638
SinI GGWCC 1 cut(s) 610
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 3 cut(s) 722, 816, 828
SsiI CCGC 3 cut(s) 439, 441, 662
StyD4I CCNGG 3 cut(s) 61, 205, 804
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 5 cut(s) 128, 151, 406, 453, 688
TaiI ACGT 3 cut(s) 178, 639, 717
TaqI TCGA 7 cut(s) 411, 531, 633, 639, 654, 711, 717
TasI AATT 3 cut(s) 722, 816, 828
TauI GCSGC 2 cut(s) 444, 664
TfiI GAWTC 3 cut(s) 41, 492, 604
TscAI CASTG 1 cut(s) 691
TseFI GTSAC 1 cut(s) 746
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 349, 605
TspRI CASTG 1 cut(s) 691
Van91I CCANNNNNTGG 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 610
XmiI GTMKAC 2 cut(s) 543, 639
ZraI GACGTC 2 cut(s) 637, 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.