pycom11g04260

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003100
Physical Location & Seq
Forward (+)
3305937 .. 3309619
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 915 bp
ATGAGAGCAGTAAAGAATACAAATAGAGGAAGAAAACACTCAGTCGCAGCCACACCCGCAGCGGAACAGGGAAACCAGCTCTCCGTTACATCAAACATTATGGCGGACGACAATCAGAATGAGGTGAAGGACGAGGTGGTAGCAGATATTGCTCCCTTTGATCCTACTAAAAAGAAGAAAAAGAAGAAGGTTGTTATTCAGGATACTACTGATGATTCTGTGGACAAGTTGGCCGAGAAAACGGAAACCTTGACAGTTTCTGAGGGGCAGGAGAGTACGTTTACTGGTTTGAAAAAGAAGAAGAAGAAGCCAGTTGAGACCAGTATCTTGAATGAAGAGAGTGGTGACCCAGTGGAAGATTTGAATGAGCGCACTGGTGAGGATGAGGAAGGAGAAGGGATTGTTCTAGAGACTCCATCATATCCTTGGGAAGGGAGTGATCGTGATTACACTTATGAAGAACTTCTTGATAGGGTGTTTAACATTCTTCGCGAAAATAACCCTGATCTGGCTGGAGACAGGCGTAGGACAGTGATGAGGCCTCCACAAGTTCTTCGTGAGGGCACAAAGAAAACGGTTTTTGTGAATTTCATGGATCTGTGCAAGACGATGCATAGGCAACCAGATCACGTCATGGCTTTCTTGCTTGCCGAACTGGGAACTAGTGGATCACTTGATGGACAGCAGAGGTTGGTTGTTAAGGGAAGATTTGCCCCAAAAAATTTCGAAGGAATACTGCGGCGATATGTCAATGAATATGTCATTTGCCTTGGGTGCAAAAGTCCAGACACTATACTTTCAAAGGAGAATCGTCTCTTCTTTCTCAGATGCGAGAAGTGTGGTTCTGGACGATCAGTTGCTCCGATTAAAGCTGGTTTTATGGCTCGGGTTGGGCGAAGGAACGCCGGGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
eIF-5_eIF-2B

Protein Analysis

305

Amino Acids

34.07

Weight (kDa)

8.16

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-5_eIF-2B PF01873 173 - 282 1.4e-39 Domain found in IF2B/IF5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 492
AciI CCGC 4 cut(s) 57, 62, 104, 739
AclWI GGATC 3 cut(s) 155, 603, 676
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 2 cut(s) 586, 721
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 2 cut(s) 431, 508
AgsI TTSAA 4 cut(s) 292, 331, 364, 801
AhlI ACTAGT 1 cut(s) 662
AjiI CACGTC 1 cut(s) 631
AluBI AGCT 2 cut(s) 79, 872
AluI AGCT 2 cut(s) 79, 872
Alw26I GTCTC 4 cut(s) 311, 404, 510, 818
AlwI GGATC 3 cut(s) 155, 603, 676
AlwNI CAGNNNCTG 1 cut(s) 260
Ama87I CYCGRG 1 cut(s) 885
AoxI GGCC 2 cut(s) 231, 539
ApeKI GCWGC 2 cut(s) 47, 59
ApoI RAATTY 2 cut(s) 586, 721
Asp700I GAANNNNTTC 1 cut(s) 462
AspLEI GCGC 1 cut(s) 372
AsuC2I CCSGG 1 cut(s) 907
AsuHPI GGTGA 3 cut(s) 136, 356, 389
AsuII TTCGAA 1 cut(s) 726
AvaI CYCGRG 1 cut(s) 885
BaeGI GKGCMC 1 cut(s) 566
BbvI GCAGC 2 cut(s) 59, 71
BccI CCATC 2 cut(s) 424, 671
BciVI GTATCC 1 cut(s) 196
BcnI CCSGG 1 cut(s) 907
BcoDI GTCTC 4 cut(s) 311, 404, 510, 818
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 2 cut(s) 407, 663
BfuI GTATCC 1 cut(s) 196
BisI GCNGC 3 cut(s) 48, 60, 740
BlsI GCNGC 3 cut(s) 49, 61, 741
Bme1390I CCNGG 1 cut(s) 907
BmeT110I CYCGRG 1 cut(s) 885
BmgBI CACGTC 1 cut(s) 631
BmrFI CCNGG 1 cut(s) 907
BmrI ACTGGG 2 cut(s) 344, 665
BmsI GCATC 2 cut(s) 600, 818
BmuI ACTGGG 2 cut(s) 344, 665
BpmI CTGGAG 1 cut(s) 534
Bpu14I TTCGAA 1 cut(s) 726
BpuMI CCSGG 1 cut(s) 907
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 425, 769
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 263, 293, 427, 457
Bsc4I CCNNNNNNNGG 2 cut(s) 431, 508
Bse1I ACTGG 6 cut(s) 289, 311, 321, 350, 379, 660
BseDI CCNNGG 2 cut(s) 425, 769
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 2 cut(s) 431, 508
BseMII CTCAG 3 cut(s) 54, 252, 838
BseNI ACTGG 6 cut(s) 289, 311, 321, 350, 379, 660
BseSI GKGCMC 1 cut(s) 566
BseXI GCAGC 2 cut(s) 59, 71
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 2 cut(s) 233, 541
BsiHKCI CYCGRG 1 cut(s) 885
BsiSI CCGG 1 cut(s) 906
BslI CCNNNNNNNGG 2 cut(s) 431, 508
BsmAI GTCTC 4 cut(s) 311, 404, 510, 818
BsmBI CGTCTC 1 cut(s) 818
BsnI GGCC 2 cut(s) 233, 541
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 885
Bsp119I TTCGAA 1 cut(s) 726
Bsp1286I GDGCHC 1 cut(s) 566
Bsp143I GATC 7 cut(s) 160, 439, 505, 595, 625, 668, 851
Bsp68I TCGCGA 1 cut(s) 492
BspACI CCGC 4 cut(s) 57, 62, 104, 739
BspANI GGCC 2 cut(s) 233, 541
BspCNI CTCAG 3 cut(s) 53, 253, 837
BspFNI CGCG 1 cut(s) 492
BspPI GGATC 3 cut(s) 155, 603, 676
BspT104I TTCGAA 1 cut(s) 726
BspTNI GGTCTC 1 cut(s) 311
BsrI ACTGG 6 cut(s) 289, 311, 321, 350, 379, 660
BssECI CCNNGG 2 cut(s) 425, 769
BssMI GATC 7 cut(s) 160, 439, 505, 595, 625, 668, 851
BssT1I CCWWGG 2 cut(s) 425, 769
Bst4CI ACNGT 3 cut(s) 256, 532, 577
Bst6I CTCTTC 2 cut(s) 330, 821
BstAPI GCANNNNNTGC 1 cut(s) 149
BstBI TTCGAA 1 cut(s) 726
BstC8I GCNNGC 1 cut(s) 648
BstDEI CTNAG 3 cut(s) 40, 261, 824
BstEII GGTNACC 1 cut(s) 344
BstENI CCTNNNNNAGG 1 cut(s) 429
BstF5I GGATG 1 cut(s) 388
BstFNI CGCG 1 cut(s) 492
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 7 cut(s) 163, 442, 508, 598, 628, 671, 854
BstMAI GTCTC 4 cut(s) 311, 404, 510, 818
BstMBI GATC 7 cut(s) 160, 439, 505, 595, 625, 668, 851
BstMWI GCNNNNNNNGC 3 cut(s) 56, 149, 774
BstPI GGTNACC 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 905
BstSLI GKGCMC 1 cut(s) 566
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 2 cut(s) 59, 71
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BsuI GTATCC 1 cut(s) 196
BsuRI GGCC 2 cut(s) 233, 541
BtrI CACGTC 1 cut(s) 631
BtsCI GGATG 1 cut(s) 388
BtsIMutI CAGTG 3 cut(s) 357, 372, 537
BtuMI TCGCGA 1 cut(s) 492
Cac8I GCNNGC 1 cut(s) 648
CaiI CAGNNNCTG 1 cut(s) 260
CfoI GCGC 1 cut(s) 372
Csp6I GTAC 1 cut(s) 276
CviAII CATG 2 cut(s) 592, 634
CviJI RGCY 9 cut(s) 50, 79, 233, 310, 512, 541, 638, 872, 884
CviKI_1 RGCY 9 cut(s) 50, 79, 233, 310, 512, 541, 638, 872, 884
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 3 cut(s) 40, 261, 824
DpnI GATC 7 cut(s) 162, 441, 507, 597, 627, 670, 853
DpnII GATC 7 cut(s) 160, 439, 505, 595, 625, 668, 851
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 330, 821
EarI CTCTTC 2 cut(s) 330, 821
EciI GGCGGA 1 cut(s) 119
Eco130I CCWWGG 2 cut(s) 425, 769
Eco147I AGGCCT 1 cut(s) 541
Eco31I GGTCTC 1 cut(s) 311
Eco88I CYCGRG 1 cut(s) 885
Eco91I GGTNACC 1 cut(s) 344
EcoNI CCTNNNNNAGG 1 cut(s) 429
EcoO65I GGTNACC 1 cut(s) 344
EcoT14I CCWWGG 2 cut(s) 425, 769
EcoT22I ATGCAT 1 cut(s) 615
ErhI CCWWGG 2 cut(s) 425, 769
Esp3I CGTCTC 1 cut(s) 818
FaeI CATG 2 cut(s) 595, 637
FalI AAGNNNNNCTT 2 cut(s) 450, 482
FatI CATG 2 cut(s) 591, 633
FauI CCCGC 1 cut(s) 64
Fnu4HI GCNGC 3 cut(s) 48, 60, 740
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 3 cut(s) 48, 60, 740
FspBI CTAG 2 cut(s) 407, 663
GlaI GCGC 1 cut(s) 371
GluI GCNGC 3 cut(s) 48, 60, 740
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 2 cut(s) 233, 541
HapII CCGG 1 cut(s) 906
HhaI GCGC 1 cut(s) 372
Hin1II CATG 2 cut(s) 595, 637
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HinfI GANTC 3 cut(s) 215, 412, 808
HpaII CCGG 1 cut(s) 906
HphI GGTGA 3 cut(s) 136, 356, 389
Hpy166II GTNNAC 2 cut(s) 223, 282
Hpy188I TCNGA 4 cut(s) 117, 262, 827, 864
Hpy188III TCNNGA 9 cut(s) 200, 328, 407, 443, 467, 491, 557, 785, 846
Hpy8I GTNNAC 2 cut(s) 223, 282
HpyAV CCTTC 7 cut(s) 121, 181, 383, 389, 425, 722, 891
HpyCH4III ACNGT 3 cut(s) 256, 532, 577
HpyCH4IV ACGT 2 cut(s) 278, 630
HpyCH4V TGCA 3 cut(s) 603, 613, 777
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 149, 774
HpyF3I CTNAG 3 cut(s) 40, 261, 824
HpySE526I ACGT 2 cut(s) 278, 630
Hsp92II CATG 2 cut(s) 595, 637
HspAI GCGC 1 cut(s) 370
Kzo9I GATC 7 cut(s) 160, 439, 505, 595, 625, 668, 851
LmnI GCTCC 2 cut(s) 157, 865
Lsp1109I GCAGC 2 cut(s) 59, 71
LweI GCATC 2 cut(s) 600, 818
MaeI CTAG 2 cut(s) 407, 663
MaeII ACGT 2 cut(s) 278, 630
MaeIII GTNAC 2 cut(s) 85, 344
MalI GATC 7 cut(s) 162, 441, 507, 597, 627, 670, 853
MboI GATC 7 cut(s) 160, 439, 505, 595, 625, 668, 851
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 566
MluCI AATT 2 cut(s) 586, 721
MlyI GAGTC 1 cut(s) 406
Mph1103I ATGCAT 1 cut(s) 615
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 3 cut(s) 480, 699, 867
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 1 cut(s) 906
MspR9I CCNGG 1 cut(s) 907
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 3 cut(s) 56, 149, 774
NciI CCSGG 1 cut(s) 907
NdeII GATC 7 cut(s) 160, 439, 505, 595, 625, 668, 851
NlaIII CATG 2 cut(s) 595, 637
NmeAIII GCCGAG 1 cut(s) 259
NmuCI GTSAC 1 cut(s) 344
NruI TCGCGA 1 cut(s) 492
NsiI ATGCAT 1 cut(s) 615
NspV TTCGAA 1 cut(s) 726
PceI AGGCCT 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 892
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 2 cut(s) 215, 808
PkrI GCNGC 3 cut(s) 49, 61, 741
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PspEI GGTNACC 1 cut(s) 344
PstNI CAGNNNCTG 1 cut(s) 260
PsuI RGATCY 1 cut(s) 595
RruI TCGCGA 1 cut(s) 492
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
SaqAI TTAA 3 cut(s) 480, 699, 867
SatI GCNGC 3 cut(s) 48, 60, 740
Sau3AI GATC 7 cut(s) 160, 439, 505, 595, 625, 668, 851
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 1 cut(s) 907
SduI GDGCHC 1 cut(s) 566
SetI ASST 9 cut(s) 81, 126, 138, 192, 251, 281, 633, 692, 874
SfaNI GCATC 2 cut(s) 600, 818
SfuI TTCGAA 1 cut(s) 726
SpeI ACTAGT 1 cut(s) 662
Sse9I AATT 2 cut(s) 586, 721
SseBI AGGCCT 1 cut(s) 541
SsiI CCGC 4 cut(s) 57, 62, 104, 739
SspMI CTAG 2 cut(s) 407, 663
StuI AGGCCT 1 cut(s) 541
StyD4I CCNGG 1 cut(s) 905
StyI CCWWGG 2 cut(s) 425, 769
TaaI ACNGT 3 cut(s) 256, 532, 577
TaiI ACGT 2 cut(s) 281, 633
TaqI TCGA 1 cut(s) 726
TasI AATT 2 cut(s) 586, 721
TauI GCSGC 1 cut(s) 742
TfiI GAWTC 2 cut(s) 215, 808
Tru1I TTAA 3 cut(s) 480, 699, 867
Tru9I TTAA 3 cut(s) 480, 699, 867
TscAI CASTG 3 cut(s) 357, 379, 537
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 2 cut(s) 47, 59
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 4 cut(s) 348, 471, 580, 768
TspGWI ACGGA 2 cut(s) 73, 257
TspRI CASTG 3 cut(s) 357, 379, 537
XagI CCTNNNNNAGG 1 cut(s) 429
XapI RAATTY 2 cut(s) 586, 721
XbaI TCTAGA 1 cut(s) 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 2 cut(s) 407, 663
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.