Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0003802

Physical Location & Seq

Genomic Coordinates Forward (+)

8980574 .. 8983381

Gene Structure 2808 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2187 bp

ATGAAATCTGTTGGTGAGTCAATGGCGCTGGGTCGCACGTTTCAAGAGTCTTTTCAGAAAGCAGTTCGATCTTTGGAATGTGGTTTCTCTGGATGGGGCTGTGCTAAAATTAAGGAGCTTGACTGGGATTGGGAACAATTGAAGTATAGCCTCCGAGTCCCTAACCCAGAGCGTATCCATGCCATATATGCTGCAATGAAGAGGGGTATGAAGGTCGATGATATTCATGAGTTGAGTTTCATTGACAAATGGTTCCTCACACAGCTTAAGGAGCTGGTAGATGTGGAGCAATACCTTCTGGCAAGAAACTTGTCTGATTTAACAAAGGATGAGTTCTACGAAGTGAAAAAGAGAGGTTTCAGTGACAAACAGATAGCTTTTGCCACTAAATCCTCTGAAAAAGACGTCAGGATGAAGAGACTATCACTAGGTGTTGCTCCATCATATAAGCGGGTGGATACTTGTGCTGCAGAATTTGAGGCCAATACTCCTTACATGTACTCTTCCTATGACTATGAGTGTGAATCAGCTCCCACCCAAAGTAAGAAGGTTTTGATTTTAGGTGGAGGACCAAATCGAATCGGTCAAGGGATCGAGTTTGACTACTGTTGTTGCCATACGTCCTTTGCACTTCAGAAAGCAGGATATGAGACAATTATGATGAATTCAAATCCTGAAACGGTATCCACAGATTATGACACAAGTGACCGTCTATATTTTGAGCCACTGACTGTCGAAGACGTGCTGAATATAATTGATCTGGAAAGACCTGATGGTATCATTGTGCAGTTTGGAGGTCAAACACCGCTGAAGCTGTCTCTTCCTATTCAGCAGTATCTAGATGAGAACAAGCCTGAATGTGCTAGTGGGAATGGACATGTACGCATTTGGGGTACTTCACCGGCTTCCATAGACGATGCTGAGGACAGGGAAAAGTTCAATACAATCCTTAATGAGTTAAAGATTGAACAACCAAAAGGAGGTATTGCCAGGAGCGAAGCTGATGCTGTTGCCATCGCTAAGGACATCGGGTACCCAGTTGTTGTCCGGCCTTCGTATGTATTGGGTGGCCGAGCCATGGAGATTGTATATAGCGACAAAAAACTTGCGACCTACCTCGAAAATGCTGTTGAAGTGGACCCAGAGCGCCCTGTGTTGATTGACAAGTATCTATCTGATGCCATTGAGATTGATGTCGATGCACTTGCCGACTCTCAGGGGAACGTTGTCATTGGTGGGATAATGGAGCACATTGAACAGGCTGGAGTCCATTCTGGTGACTCTGCTTGCTCGATTCCAACAAAAACCATCCCTGCTTCTTGCTTGGACACAATTAGGACATGGACTACAAAATTGGCTAGGAGGCTGAATGTATGTGGGCTGATGAACTGTCAGTATGCAATTACCTTGTCTGGAGACGTTTTCTTACTCGAGGCAAACCCTCGTGCTTCCCGTACTGTCCCTTTTGTGTCCAAGGCGATTGGGCATCCATTGGCTAAATATGCTTCTCTTGTCATGTCTGGGAAGTCTCTTCATGATCGAAATTTCACAAAAGAGGTCATTCCTGCACATGTATCAGTAAAGGAAGCTGTTCTTCCATTTGAGAAGTTCCCAGGATGTGATGTGTTGCTGGGGCCTGAGATGCGTAGTACTGGGGAGGTGATGGGTATTGATTACGAGTTTCCCATTGCATTCGCCAAGGCTCAGATTTCTGCAGGGCAGAAGCTGCCACTTACTGGCACTGTGTTCCTCAGCTTAAATGATTTGACCAAACCTCATCTTGAAAAGATTGCCAAAGCCTTTTTGGGACTTGGATTTAAGATTGTTTCAACTTCTGGGACAGCTCATATTCTTGAATTAGCAAAACTTCCAGTGGAACGAGTGCTCAAACTTCATGAAGGACGGCCTCATGCCGCTGATATGGTTGCTAATGGCCAGATCCAGTTAATGGTTATCACAAGTTCGGGTGATGCACTTGATCAGATTGATGGACGGCAACTGAGGAGGTCGGGCCTTGCTTACAAGATTCCAGTAATAACAACTATTGCCGGAGCTTTGTCAACTGCAGAAGCAATAAAGAGCTTAAAGTCTAGCACTATTAAGATGATTGCACTGCAGGACTTCTTTGAGGATGAGAACAAAGCTGGGAGTGATAAAACATTGCAGTCAGTCACTTCCTCCCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004087 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_L_D2 CPSase_L_D3 MGS

Protein Analysis

729

Amino Acids

80.21

Weight (kDa)

5.58

Isoelectric Point (pI)

38.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_L_D3	PF02787	50 - 128	1.4e-27	Carbamoyl-phosphate synthetase large chain, oligomerisation domain
CPSase_L_D1	PF25596	181 - 305	3.3e-45	Carbamoyl phosphate synthase preATP-grasp domain
CPSase_L_D2	PF02786	309 - 512	9.6e-37	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	317 - 483	1.3e-06	ATP-grasp domain
Dala_Dala_lig_C	PF07478	336 - 481	6e-07	D-ala D-ala ligase C-terminus
MGS	PF02142	594 - 680	1.3e-18	MGS-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29900
fragaria_vesca	FvH4_1g25660 FvH4_1g25660
malus_domestica	MD03G1111400.v1.1 MD11G1125100.v1.1 MD12G1166200.v1.1
prunus_persica	Prupe.6G093800_v2.0.a1
pyrus_communis	pycom11g10560 pycom11g10570
rosa_chinensis	RchiOBHm_Chr2g0124141 RchiOBHm_Chr7g0219181
rosa_laevigata	RLG00000002304 RLG00000018786
rosa_multiflora	Rmu_co8484899.1_g000001 Rmu_sc0003840.1_g000003 Rmu_sc0008812.1_g000002 Rmu_sc0009785.1_g000029
rosa_roxburghii	Rroxscaffold_2G00120100 Rroxscaffold_3G00240170
rosa_rugosa	Rorug02G0248800 Rorug07G0182900 Rorug07G0183000 Rorug07G0183000
rosa_samantha	Rh2AG307800 Rh2BG316800 Rh2CG294600 Rh2DG331600 Rh2DG331800 Rh7AG326600 Rh7BG316400 Rh7CG343900 Rh7DG321900
rosa_wichuraiana	Rw2G024810 Rw7G027510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	408
Acc65I	GGTACC	1 cut(s)	1032
AccB1I	GGYRCC	1 cut(s)	1032
AccB7I	CCANNNNNTGG	2 cut(s)	1736, 1948
AciI	CCGC	3 cut(s)	451, 806, 1914
AclI	AACGTT	1 cut(s)	1224
AclWI	GGATC	2 cut(s)	599, 1933
AcoI	YGGCCR	2 cut(s)	1069, 1933
AcsI	RAATTY	3 cut(s)	473, 664, 1543
AcuI	CTGAAG	2 cut(s)	617, 830
AcyI	GRCGYC	1 cut(s)	405
AdeI	CACNNNGTG	1 cut(s)	431
AfaI	GTAC	6 cut(s)	500, 882, 895, 1034, 1456, 1651
AfiI	CCNNNNNNNGG	4 cut(s)	980, 1078, 1736, 1948
AflII	CTTAAG	1 cut(s)	266
AflIII	ACRYGT	3 cut(s)	495, 877, 1570
AjiI	CACGTC	1 cut(s)	742
AjnI	CCWGG	2 cut(s)	989, 1612
Alw21I	GWGCWC	2 cut(s)	1251, 1887
Alw26I	GTCTC	5 cut(s)	412, 644, 822, 1410, 1533
AlwI	GGATC	2 cut(s)	599, 1933
AlwNI	CAGNNNCTG	1 cut(s)	1726
Ama87I	CYCGRG	1 cut(s)	1430
AoxI	GGCC	7 cut(s)	480, 1049, 1069, 1634, 1904, 1933, 2011
ApeKI	GCWGC	3 cut(s)	191, 467, 1726
ApoI	RAATTY	3 cut(s)	473, 664, 1543
Asp700I	GAANNNNTTC	1 cut(s)	1590
Asp718I	GGTACC	1 cut(s)	1032
AspLEI	GCGC	2 cut(s)	28, 1149
AspS9I	GGNCC	4 cut(s)	569, 1138, 1634, 2011
AsuHPI	GGTGA	5 cut(s)	26, 891, 1289, 1672, 1979
AvaI	CYCGRG	1 cut(s)	1430
AvaII	GGWCC	2 cut(s)	569, 1138
BalI	TGGCCA	1 cut(s)	1935
BanI	GGYRCC	1 cut(s)	1032
BauI	CACGAG	1 cut(s)	1443
BbsI	GAAGAC	1 cut(s)	744
Bbv12I	GWGCWC	2 cut(s)	1251, 1887
BbvCI	CCTCAGC	2 cut(s)	921, 1751
BbvI	GCAGC	3 cut(s)	178, 454, 1713
BccI	CCATC	7 cut(s)	87, 448, 767, 1022, 1316, 1657, 1982
BceAI	ACGGC	2 cut(s)	1919, 2009
BcgI	CGANNNNNNTGC	4 cut(s)	986, 1020, 1187, 1221
BciT130I	CCWGG	2 cut(s)	991, 1614
BciVI	GTATCC	3 cut(s)	185, 451, 694
BclI	TGATCA	1 cut(s)	1978
BcoDI	GTCTC	5 cut(s)	412, 644, 822, 1410, 1533
BfaI	CTAG	5 cut(s)	428, 839, 864, 1359, 2091
BfmI	CTRYAG	4 cut(s)	468, 1713, 2064, 2114
BfoI	RGCGCY	2 cut(s)	29, 1150
BfrI	CTTAAG	1 cut(s)	266
BfuI	GTATCC	3 cut(s)	185, 451, 694
BisI	GCNGC	4 cut(s)	192, 468, 1727, 1914
BlsI	GCNGC	4 cut(s)	193, 469, 1728, 1915
BmcAI	AGTACT	1 cut(s)	1651
Bme1390I	CCNGG	2 cut(s)	991, 1614
Bme18I	GGWCC	2 cut(s)	569, 1138
BmeT110I	CYCGRG	1 cut(s)	1430
BmgBI	CACGTC	1 cut(s)	742
BmgT120I	GGNCC	4 cut(s)	569, 1138, 1634, 2011
BmiI	GGNNCC	4 cut(s)	254, 1034, 1140, 1635
BmrFI	CCNGG	2 cut(s)	991, 1614
BmrI	ACTGGG	3 cut(s)	133, 1031, 1662
BmsI	GCATC	7 cut(s)	907, 994, 1168, 1189, 1495, 1632, 1960
BmuI	ACTGGG	3 cut(s)	133, 1031, 1662
BpiI	GAAGAC	1 cut(s)	744
BpmI	CTGGAG	2 cut(s)	1284, 1434
Bpu10I	CCTNAGC	3 cut(s)	921, 1020, 1751
BsaHI	GRCGYC	1 cut(s)	405
BsaJI	CCNNGG	4 cut(s)	1077, 1473, 1612, 1698
Bsc4I	CCNNNNNNNGG	4 cut(s)	980, 1078, 1736, 1948
Bse118I	RCCGGY	1 cut(s)	901
Bse1I	ACTGG	7 cut(s)	128, 1037, 1657, 1741, 1871, 1942, 2030
Bse3DI	GCAATG	3 cut(s)	201, 1686, 2159
BseBI	CCWGG	2 cut(s)	991, 1614
BseDI	CCNNGG	4 cut(s)	1077, 1473, 1612, 1698
BseGI	GGATG	7 cut(s)	98, 334, 417, 1308, 1486, 1622, 2137
BseLI	CCNNNNNNNGG	4 cut(s)	980, 1078, 1736, 1948
BseMI	GCAATG	3 cut(s)	201, 1686, 2159
BseMII	CTCAG	6 cut(s)	912, 1229, 1629, 1718, 1765, 1991
BseNI	ACTGG	7 cut(s)	128, 1037, 1657, 1741, 1871, 1942, 2030
BseRI	GAGGAG	1 cut(s)	2017
BseXI	GCAGC	3 cut(s)	178, 454, 1713
BseYI	CCCAGC	3 cut(s)	28, 1630, 2144
BsgI	GTGCAG	2 cut(s)	806, 1551
BshFI	GGCC	7 cut(s)	482, 1051, 1071, 1636, 1906, 1935, 2013
BshNI	GGYRCC	1 cut(s)	1032
BsiHKAI	GWGCWC	2 cut(s)	1251, 1887
BsiHKCI	CYCGRG	1 cut(s)	1430
BsiSI	CCGG	3 cut(s)	902, 1048, 2049
BslFI	GGGAC	4 cut(s)	143, 1445, 1821, 1852
BslI	CCNNNNNNNGG	4 cut(s)	980, 1078, 1736, 1948
BsmAI	GTCTC	5 cut(s)	412, 644, 822, 1410, 1533
BsmBI	CGTCTC	1 cut(s)	1410
BsmFI	GGGAC	4 cut(s)	143, 1445, 1821, 1852
BsmI	GAATGC	1 cut(s)	1691
BsnI	GGCC	7 cut(s)	482, 1051, 1071, 1636, 1906, 1935, 2013
BsoBI	CYCGRG	1 cut(s)	1430
Bsp1286I	GDGCHC	2 cut(s)	1251, 1887
Bsp143I	GATC	6 cut(s)	68, 591, 757, 1537, 1938, 1978
Bsp19I	CCATGG	1 cut(s)	1077
BspACI	CCGC	3 cut(s)	451, 806, 1914
BspANI	GGCC	7 cut(s)	482, 1051, 1071, 1636, 1906, 1935, 2013
BspCNI	CTCAG	6 cut(s)	913, 1228, 1630, 1717, 1764, 1992
BspHI	TCATGA	3 cut(s)	226, 1534, 1894
BspLI	GGNNCC	4 cut(s)	254, 1034, 1140, 1635
BspMAI	CTGCAG	4 cut(s)	472, 1717, 2068, 2118
BspPI	GGATC	2 cut(s)	599, 1933
BspT107I	GGYRCC	1 cut(s)	1032
BspTI	CTTAAG	1 cut(s)	266
BsrDI	GCAATG	3 cut(s)	201, 1686, 2159
BsrFI	RCCGGY	1 cut(s)	901
BsrI	ACTGG	7 cut(s)	128, 1037, 1657, 1741, 1871, 1942, 2030
BssAI	RCCGGY	1 cut(s)	901
BssECI	CCNNGG	4 cut(s)	1077, 1473, 1612, 1698
BssMI	GATC	6 cut(s)	68, 591, 757, 1537, 1938, 1978
BssNI	GRCGYC	1 cut(s)	405
BssSI	CACGAG	1 cut(s)	1443
BssT1I	CCWWGG	3 cut(s)	1077, 1473, 1698
Bst2BI	CACGAG	1 cut(s)	1443
Bst2UI	CCWGG	2 cut(s)	991, 1614
Bst4CI	ACNGT	7 cut(s)	608, 682, 710, 733, 1391, 1459, 1744
Bst6I	CTCTTC	5 cut(s)	194, 410, 508, 825, 1536
BstACI	GRCGYC	1 cut(s)	405
BstAFI	CTTAAG	1 cut(s)	266
BstAPI	GCANNNNNTGC	1 cut(s)	1726
BstC8I	GCNNGC	1 cut(s)	1288
BstDEI	CTNAG	7 cut(s)	921, 1020, 1215, 1638, 1704, 1751, 2000
BstDSI	CCRYGG	1 cut(s)	1077
BstF5I	GGATG	7 cut(s)	98, 334, 417, 1308, 1486, 1622, 2137
BstH2I	RGCGCY	2 cut(s)	29, 1150
BstHHI	GCGC	2 cut(s)	28, 1149
BstKTI	GATC	6 cut(s)	71, 594, 760, 1540, 1941, 1981
BstMAI	GTCTC	5 cut(s)	412, 644, 822, 1410, 1533
BstMBI	GATC	6 cut(s)	68, 591, 757, 1537, 1938, 1978
BstMWI	GCNNNNNNNGC	5 cut(s)	188, 271, 1502, 1642, 1726
BstNI	CCWGG	2 cut(s)	991, 1614
BstNSI	RCATGY	3 cut(s)	499, 881, 1574
BstSCI	CCNGG	2 cut(s)	989, 1612
BstSFI	CTRYAG	4 cut(s)	468, 1713, 2064, 2114
BstV1I	GCAGC	3 cut(s)	178, 454, 1713
BstV2I	GAAGAC	1 cut(s)	744
BstX2I	RGATCY	1 cut(s)	1938
BstYI	RGATCY	1 cut(s)	1938
BsuI	GTATCC	3 cut(s)	185, 451, 694
BsuRI	GGCC	7 cut(s)	482, 1051, 1071, 1636, 1906, 1935, 2013
BtgI	CCRYGG	1 cut(s)	1077
BtgZI	GCGATG	1 cut(s)	1000
BtrI	CACGTC	1 cut(s)	742
BtsCI	GGATG	7 cut(s)	98, 334, 417, 1308, 1486, 1622, 2137
BtsI	GCAGTG	1 cut(s)	2111
BtsIMutI	CAGTG	5 cut(s)	367, 725, 1740, 1878, 2111
Cac8I	GCNNGC	1 cut(s)	1288
CaiI	CAGNNNCTG	1 cut(s)	1726
CciI	TCATGA	3 cut(s)	226, 1534, 1894
CfoI	GCGC	2 cut(s)	28, 1149
Cfr10I	RCCGGY	1 cut(s)	901
Cfr13I	GGNCC	4 cut(s)	569, 1138, 1634, 2011
Csp6I	GTAC	6 cut(s)	499, 881, 894, 1033, 1455, 1650
CviQI	GTAC	6 cut(s)	499, 881, 894, 1033, 1455, 1650
DdeI	CTNAG	7 cut(s)	921, 1020, 1215, 1638, 1704, 1751, 2000
DpnI	GATC	6 cut(s)	70, 593, 759, 1539, 1940, 1980
DpnII	GATC	6 cut(s)	68, 591, 757, 1537, 1938, 1978
DraIII	CACNNNGTG	1 cut(s)	431
EaeI	YGGCCR	2 cut(s)	1069, 1933
Eam1104I	CTCTTC	5 cut(s)	194, 410, 508, 825, 1536
EarI	CTCTTC	5 cut(s)	194, 410, 508, 825, 1536
Eco130I	CCWWGG	3 cut(s)	1077, 1473, 1698
Eco47I	GGWCC	2 cut(s)	569, 1138
Eco57I	CTGAAG	2 cut(s)	617, 830
Eco88I	CYCGRG	1 cut(s)	1430
EcoO109I	RGGNCCY	1 cut(s)	1634
EcoRI	GAATTC	1 cut(s)	664
EcoRII	CCWGG	2 cut(s)	989, 1612
EcoT14I	CCWWGG	3 cut(s)	1077, 1473, 1698
ErhI	CCWWGG	3 cut(s)	1077, 1473, 1698
Esp3I	CGTCTC	1 cut(s)	1410
FalI	AAGNNNNNCTT	2 cut(s)	1578, 1610
FaqI	GGGAC	4 cut(s)	143, 1445, 1821, 1852
FauI	CCCGC	1 cut(s)	444
FbaI	TGATCA	1 cut(s)	1978
Fnu4HI	GCNGC	4 cut(s)	192, 468, 1727, 1914
FokI	GGATG	7 cut(s)	105, 341, 424, 1295, 1473, 1629, 2144
Fsp4HI	GCNGC	4 cut(s)	192, 468, 1727, 1914
FspBI	CTAG	5 cut(s)	428, 839, 864, 1359, 2091
GlaI	GCGC	2 cut(s)	27, 1148
GluI	GCNGC	4 cut(s)	192, 468, 1727, 1914
GsaI	CCCAGC	3 cut(s)	32, 1634, 2148
GsuI	CTGGAG	2 cut(s)	1284, 1434
HaeII	RGCGCY	2 cut(s)	29, 1150
HaeIII	GGCC	7 cut(s)	482, 1051, 1071, 1636, 1906, 1935, 2013
HapII	CCGG	3 cut(s)	902, 1048, 2049
HhaI	GCGC	2 cut(s)	28, 1149
Hin1I	GRCGYC	1 cut(s)	405
Hin6I	GCGC	2 cut(s)	26, 1147
HinP1I	GCGC	2 cut(s)	26, 1147
HincII	GTYRAC	1 cut(s)	2061
HindII	GTYRAC	1 cut(s)	2061
HpaII	CCGG	3 cut(s)	902, 1048, 2049
HphI	GGTGA	5 cut(s)	26, 891, 1289, 1672, 1979
Hpy166II	GTNNAC	2 cut(s)	1138, 2061
Hpy188I	TCNGA	9 cut(s)	57, 155, 316, 397, 636, 1177, 1707, 1983, 2186
Hpy8I	GTNNAC	2 cut(s)	1138, 2061
HpyAV	CCTTC	5 cut(s)	205, 305, 541, 1062, 1892
HpyCH4III	ACNGT	7 cut(s)	608, 682, 710, 733, 1391, 1459, 1744
HpyCH4IV	ACGT	6 cut(s)	38, 405, 620, 741, 1224, 1419
HpyF10VI	GCNNNNNNNGC	5 cut(s)	188, 271, 1502, 1642, 1726
HpyF3I	CTNAG	7 cut(s)	921, 1020, 1215, 1638, 1704, 1751, 2000
HpySE526I	ACGT	6 cut(s)	38, 405, 620, 741, 1224, 1419
Hsp92I	GRCGYC	1 cut(s)	405
HspAI	GCGC	2 cut(s)	26, 1147
KpnI	GGTACC	1 cut(s)	1036
Ksp22I	TGATCA	1 cut(s)	1978
Kzo9I	GATC	6 cut(s)	68, 591, 757, 1537, 1938, 1978
LmnI	GCTCC	8 cut(s)	115, 271, 286, 442, 535, 993, 1246, 2051
Lsp1109I	GCAGC	3 cut(s)	178, 454, 1713
LweI	GCATC	7 cut(s)	907, 994, 1168, 1189, 1495, 1632, 1960
MaeI	CTAG	5 cut(s)	428, 839, 864, 1359, 2091
MaeII	ACGT	6 cut(s)	38, 405, 620, 741, 1224, 1419
MaeIII	GTNAC	4 cut(s)	362, 704, 1277, 2170
MalI	GATC	6 cut(s)	70, 593, 759, 1539, 1940, 1980
MboI	GATC	6 cut(s)	68, 591, 757, 1537, 1938, 1978
MboII	GAAGA	7 cut(s)	211, 427, 495, 749, 812, 1523, 1586
MfeI	CAATTG	1 cut(s)	137
MflI	RGATCY	1 cut(s)	1938
MhlI	GDGCHC	2 cut(s)	1251, 1887
MlsI	TGGCCA	1 cut(s)	1935
MluNI	TGGCCA	1 cut(s)	1935
MlyI	GAGTC	6 cut(s)	26, 56, 165, 1205, 1274, 1275
MmeI	TCCRAC	1 cut(s)	1322
Mox20I	TGGCCA	1 cut(s)	1935
MroXI	GAANNNNTTC	1 cut(s)	1590
MscI	TGGCCA	1 cut(s)	1935
MslI	CAYNNNNRTG	1 cut(s)	1275
Msp20I	TGGCCA	1 cut(s)	1935
MspA1I	CMGCKG	2 cut(s)	808, 1916
MspCI	CTTAAG	1 cut(s)	266
MspI	CCGG	3 cut(s)	902, 1048, 2049
MspR9I	CCNGG	2 cut(s)	991, 1614
MunI	CAATTG	1 cut(s)	137
Mva1269I	GAATGC	1 cut(s)	1691
MvaI	CCWGG	2 cut(s)	991, 1614
MwoI	GCNNNNNNNGC	5 cut(s)	188, 271, 1502, 1642, 1726
NcoI	CCATGG	1 cut(s)	1077
NdeII	GATC	6 cut(s)	68, 591, 757, 1537, 1938, 1978
NlaIV	GGNNCC	4 cut(s)	254, 1034, 1140, 1635
NmeAIII	GCCGAG	1 cut(s)	1097
NmuCI	GTSAC	4 cut(s)	362, 704, 1277, 2170
NspI	RCATGY	3 cut(s)	499, 881, 1574
PaeR7I	CTCGAG	1 cut(s)	1430
PagI	TCATGA	3 cut(s)	226, 1534, 1894
PciI	ACATGT	3 cut(s)	495, 877, 1570
PcsI	WCGNNNNNNNCGW	1 cut(s)	1450
PctI	GAATGC	1 cut(s)	1691
PdmI	GAANNNNTTC	1 cut(s)	1590
PfeI	GAWTC	4 cut(s)	524, 579, 1294, 2026
PflMI	CCANNNNNTGG	2 cut(s)	1736, 1948
PkrI	GCNGC	4 cut(s)	193, 469, 1728, 1915
PleI	GAGTC	6 cut(s)	25, 55, 164, 1205, 1274, 1274
PpsI	GAGTC	6 cut(s)	25, 55, 164, 1205, 1274, 1274
PscI	ACATGT	3 cut(s)	495, 877, 1570
Psp1406I	AACGTT	1 cut(s)	1224
Psp6I	CCWGG	2 cut(s)	989, 1612
PspFI	CCCAGC	3 cut(s)	28, 1630, 2144
PspGI	CCWGG	2 cut(s)	989, 1612
PspN4I	GGNNCC	4 cut(s)	254, 1034, 1140, 1635
PspPI	GGNCC	4 cut(s)	569, 1138, 1634, 2011
PspXI	VCTCGAGB	1 cut(s)	1430
PstI	CTGCAG	4 cut(s)	472, 1717, 2068, 2118
PstNI	CAGNNNCTG	1 cut(s)	1726
PsuI	RGATCY	1 cut(s)	1938
RsaI	GTAC	6 cut(s)	500, 882, 895, 1034, 1456, 1651
RsaNI	GTAC	6 cut(s)	499, 881, 894, 1033, 1455, 1650
RseI	CAYNNNNRTG	1 cut(s)	1275
SatI	GCNGC	4 cut(s)	192, 468, 1727, 1914
Sau3AI	GATC	6 cut(s)	68, 591, 757, 1537, 1938, 1978
Sau96I	GGNCC	4 cut(s)	569, 1138, 1634, 2011
ScaI	AGTACT	1 cut(s)	1651
SchI	GAGTC	6 cut(s)	26, 56, 165, 1205, 1274, 1275
ScrFI	CCNGG	2 cut(s)	991, 1614
SduI	GDGCHC	2 cut(s)	1251, 1887
SfaNI	GCATC	7 cut(s)	907, 994, 1168, 1189, 1495, 1632, 1960
SfcI	CTRYAG	4 cut(s)	468, 1713, 2064, 2114
Sfr274I	CTCGAG	1 cut(s)	1430
SinI	GGWCC	2 cut(s)	569, 1138
SlaI	CTCGAG	1 cut(s)	1430
SmiMI	CAYNNNNRTG	1 cut(s)	1275
SmlI	CTYRAG	2 cut(s)	266, 1430
SmoI	CTYRAG	2 cut(s)	266, 1430
SsiI	CCGC	3 cut(s)	451, 806, 1914
SspMI	CTAG	5 cut(s)	428, 839, 864, 1359, 2091
StyD4I	CCNGG	2 cut(s)	989, 1612
StyI	CCWWGG	3 cut(s)	1077, 1473, 1698
TaaI	ACNGT	7 cut(s)	608, 682, 710, 733, 1391, 1459, 1744
TaiI	ACGT	6 cut(s)	41, 408, 623, 744, 1227, 1422
TaqII	GACCGA	1 cut(s)	572
TatI	WGTACW	2 cut(s)	498, 1649
TauI	GCSGC	1 cut(s)	1916
TfiI	GAWTC	4 cut(s)	524, 579, 1294, 2026
TscAI	CASTG	5 cut(s)	367, 732, 1747, 1878, 2118
TseFI	GTSAC	4 cut(s)	362, 704, 1277, 2170
TseI	GCWGC	3 cut(s)	191, 467, 1726
Tsp45I	GTSAC	4 cut(s)	362, 704, 1277, 2170
TspRI	CASTG	5 cut(s)	367, 732, 1747, 1878, 2118
Van91I	CCANNNNNTGG	2 cut(s)	1736, 1948
Vha464I	CTTAAG	1 cut(s)	266
VpaK11BI	GGWCC	2 cut(s)	569, 1138
XapI	RAATTY	3 cut(s)	473, 664, 1543
XbaI	TCTAGA	1 cut(s)	838
XceI	RCATGY	3 cut(s)	499, 881, 1574
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1801
XhoI	CTCGAG	1 cut(s)	1430
XmnI	GAANNNNTTC	1 cut(s)	1590
XspI	CTAG	5 cut(s)	428, 839, 864, 1359, 2091
ZraI	GACGTC	1 cut(s)	406
ZrmI	AGTACT	1 cut(s)	1651

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.