pycom11g13150

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001971
Physical Location & Seq
Forward (+)
12339271 .. 12360211
20941 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5967 bp
ATGAGGACAGATCTTCTTCAGCGTGCTGAAGTTCATGTCTGTGCATTTGATATTGAGACGACGAAGCTACCTTTGAAATTTCCAGATGCTGAATATGATTTAATAATGATGATTTCATACATGGTTGATGGACAAGGTTACTTGATTATTAACAGAGAGTGTGTTGGGGATGACATAGAGGATTTGGAGTACACTCCAAAACCAGAATTTGAAGGGTTTTTCAAGGTGACAAATGTGAAGAATGAGATAGAGCTTCTAAGAAAATGGTTTTCCCATATGCGGGAGGTGAAGCCTGGAATTTATGTCACATACAATGGTGATTTTTTCGACTGGCCTTTCCTGGAAACCAGAGCCGCGTATCATGGGTTCAAACTGAATGATGAAGTTGGATTTCAATGTGACAAGAATCAGGGGGAATGCCGAGCTAAATTTGCTTGCCATCTTGACTGTTTTGCATGGGTCAAACGTGATAGTTATCTTCCTCAAGGAAGCCAGGGCCTTAAGGCTGTTACAAAGGCAAAGTTAGGTTATCATCCGCTGGAGGTAAATCCTGAGGATATGGTTCGCTTTGCAAAGGAGAAGCCTCAGATGATGGCCTCCTATTCTGTTTCTGATGCCGTTGCGACTTATTACTTGTATATGACTTATGTTCATCCATTCATTTTCTCTCTCGCAACTATTATTCCAATGTCTCCGGACGAGGTTTTGCGCAAGGGTAGTGGAACTCTATGTGAAATGCTTCTCATGGTCCAGGCATACAAGGCAAATGTTATTTGCCCTAATAAACACCAATCTGATCCAGAAAAGTTCTATAAAAATCACCTTTTAGAGAGTGAGACTTATATAGGTGGCCATGTTGAATGCCTTGAAAGTGGTGTTTTTCGATCTGATATTCCAACTAGTTTCAAGCTTGACGCCTCTGCCTATGAGCAATTGATCAACAATCTTGATCGAGATCTTAAATATGCTATAAGAGTAGAGGGTAAGATGGACTTGGAATCAGTATCCAATTATGAAGATGTGAAGAGTTCTATATTGGAAAAGCTTATGGGGTTGCGAGATGAACCTATACGTGAAGAGTGCCCCCTTATTTATCATCTGGATGTTGCTGCAATGTATCCCAACATCATTTTGACAAACAGACTTCAGCCATCATCAATAGTTACAGACGAGATTTGCACTGCTTGTGATTTCAACCGTCCAGGCAAAACATGTCTTCGCAAGCTTGAATGGGTTTGGCGTGGAGAAATATTCATGGCAAGGAAAAGTGACTATTATCATTTGAAGAAGCAAATTGAGTCTGAGTTTGTTGACTGTGGCAATTCCAAGCTGTCAAAATCTTTTCTTGAGCTGCCTAAAATGGAACAACAGTCAAGGCTCAAAGAGCGTCTTAAAAAATACTGTCAGAAGGCGTATAGACGAGTAGTGGACAAGCCAGTCACTGAAGTTCGAGAAGCAGGGATATGCATGAGGGAAAATTCATTTTATGTTGATACAGTTCGTAGCTTTCGAGATAGAAGGTATGAGTACAAGGGGCTTAATAAGGTTTGGAAGGGGAAGTTGTCTGAAGCCAAGGCTAGTGGAAATTCTATAAAAATCCAGGAAGCACAAGATATGGTAGTGCTTTATGATTCATTACAACTTGCACATAAATGTATACTTAATTCCTTTTATGGATATGTCATGCGCAAGGGTGCAAGATGGTATTCCATGGAAATGGCTGGAGTAGTTACATATACCGGAGCCAAAATTATTCAGAATGCTCGCTTGCTGGTTGAGAAAATTGGAAAACCTCTCGAGTTAGACACAGATGGTATCTGGTGTGCACTCCCTGGATCTTTTCCAGAGAACTTTACATTCAAAACAAGAGACATGAAGAAGCTGACTATCTCATATCCATGTGTAATGCTTAATGTTGATGTGGCGAGAAACAATACAAATGACCAATACCAGACTCTTACAGATCCTGTTAAGAATACTTACACGACTCATAGCGAGTGTTCAATTGAATTTGAAGTGGATGGACCATACAAGGCAATGATTCTTCCTGCTTCTAAAGAAGAAGGCATTCTAATTAAGAAGCGATATGCTGTTTTTAATGATGATGGGACCCTTGCTGAACTTAAAGGTTTCGAGATCAAGCGTAGAGGTGAGCTGAAGCTCATCAAAGTTTTCCAGGCTGAGCTTTTTGATAAGTTCCTTCACGGTTCTACTTTAGAGGAATGCTATTTTGCAGTTGCTTCTGTCGCCAACCGTTGGCTTGATCTCCTTGATAATCAAGGAAAGGATATTGCAGACAGTGAGTTGCTGGATTATATATCAGAGTCAAGCACCATGAGCAAGTCTTTAGTAGACTATGGAGAGCAAAAGTCATGTGCAGTGACCACTGCAAGACGTCTTGCTGATTTTCTTGGTGATACGATGGTCAAAGAGAAAGGACTGCGTTGCCAATATATAGTTGCATGTGAACCAAAGGGCACACCTGTTAGTGAGCGTGCTGTTCCAGTTGCAATATTTGAAACAGATGCTGAAATTATGAAGTTTTACGTAAGGAAGTGGTGCAAGGTTTCATCAGATGTTGGCATTCGTTCCATTATTGACTGGTCCTATTACAAGCAACGCCTTGGTTCAGCAATTCAGAAAATTGTCACCATTCCGGCTGCAATGCAAAAGGTTGCAAATCCTGTTCCTAGGGTAGCTCATCCTGATTGGCTGCATAAGAAGGTTTGTGAGAAGGATGACAAATTTCGCCAGCGCAAGTTGGTTGATATCTTCAAGTCATTGAACAGGGATCATTTGCTAGAAAAGAGCAGTGATGCTATTGACTCTAATCATGTGATTGACCAAGAGATTGTGGATGATTTGGAAGACTTCAACAAAAAAAGCAGTTCCATAAGTGGACCCAGACCAATTGTTCGCTGTTATGAAGTCAATAGCAAGAAATATTCAGTCGAGACAACTTGCCAAATAAATTCACAGCAACAACAAACTAATAATAAGGAAAATGAGTGTCAAGAGCAAAATGGTGTCCCTTTAGAATGCATTGATAGAAATGTTGATTATAATGGTTGGCTTGAAATGAAGAAGAGAAAATGGAAAGATACTCTTGACAGGAGGAAGAAGCAAAGATTAAATAACGCAAGGTCTGCTCACCATGCGAATAATGAGCTGCGTGGTGGTGGTAGAAAGCAGAAAGGAACTCAGGGAAAAATAGGTGTGAATTCATATTTTACAAGGCATGAAGATGCCCTAACACGCTGTCATTGGCAGATATTACAACTAGTGCCTAGTTTTCAGGGTAACCAATTTTACGCTTGGGTGGTTACTGAAGGAATCATGATTAAGATTCCTGTTACTGTTCCAAGAGTTTTTTACATTAATTCTAAAGCACCTATTGAAAAGATATTTGAAAAGATATGTGAAGAAATACCTCCAGGAAAGGATGTCAAAAAGGCTCTTCCTCATGGAAGGCATATCTACCACCTATATGAGGCTGCAATTGATGAGGGTCGGTTTAGGACAATTAGTAAAAAGCTTGCAGCTCTTCTTGGCGATCCAGAAGTTGAGGGAATATATGAAACAAAGGTACCTTTGGAGTTCAATGCAATCGTTCAGATTGGTTGTGTCTGCAAAGTGGATAAAACTGCTAAGAAGAGAAACGTCCAAGATGGTTGGACTGTTAGTGAACTGCACATGAAGACAACTACGGAGTGTTCATATCTTGACCAGTCCATCTCATTCTTTTACTTGTATCAGAGCACATCTGAAGGGCGAGCTATATATATTGCCTATTTTCCTGCATCGGCAACAATAACGGTTGTGGTGGTAAATCCTTATCAAAATAAAGATTTGTCACCATCTTTTCTGGAAAAACTATTCCGTGATGCATGCCAAACATTATCAATTCAGCCACCTCCTAGGAATGGTATCATTATAAAGGTGGACTATGTTGGATTCGTCAAGGATGCTGAAACAATTTTGCACCGTGCAATTAATGAGTACAGACATGAACATTCCGGACCTACAATTGCTGTTATCGAATGCCCAAATTTTCAATTGATGAAGTCATGTATAGGAGCTCTTGATGATTTTCCTTGCGTGAGCATTCCCTCCAATGCTCGTGATAGTCTTTACCAGGTTCTTGGGTGGCAGCAAGTGGCTGCAAAAATTGGAATGCAGCGCTGTGCTGCATCACCTCAGTGGTTGAATGAGAGAATTTCACTCTCAAGATATGCTCATGTTCCATTGGGAAATTTCGAACTCGACTGGCTTATACATACAGCAGATATTTTCTTTTCAAGAGCACTACGTGATCAACAACAGGTGCTTTGGATATCTGATGATGGTATTCCAGATCTAGGAGGCATTAATGAAGAAGATTCATGTTTTGCTGATGAGGTCTGTGAACTTGATTTTTCTTACCCTGGAGCATATAGGAAGGTCACCGTGGAGTTAAAGATACATCACCTGGCTGTAAATGCTCTCTTAAAAAGCAACCAGGTGAATGAAATTGAAGGAGGTTCTTTGCTAGGATTTGAATCAGATGTGAACTCGGGAGTACATACTTTTGATGCACATGTTGGCTTTGATGAAGCTTCCTCATGTGCTCCTACATTCCGAGTCCTGAAACAGTTGATTCAGAGGTGTCTTTCAAATGCAGTTATGTCTGGAAATGTTTACGCTGATGCATTATTGCAACATTTATATCGATGGCTTTGCAGTCCCCAGTCTAAGCTTCATGATCCAGCTCTTCACCGCATACTTCATAAGGTCATGAAAAAGGTGCTTGCAATTTTAATGACTGAGTTCCGCAAATTGGGTGCCAAGATAATATTCGCGAATTTCTACAAGGTCATCATAGACACAGGAAAATTTGATCTGTCAGCTGCCAAAGCTTATTGTGATAGCTTGCTTAAAACTCTGCAAACGAGAGAACTATTTGAGTGGATTGAGCTTGAACCACTACACTTCTGGCATTCTTTTCTTTTCATGGACCAGTATAACTATGGTGGAATTCCGGTTAGAGATGAAGGAAGCATGGATGGTGAAGCTCAAATGGATATTGTATCAAGCTGGAATATTGCTGAATACTTACCCACGAAAATTCAGGACCACTTTGTTTATATTGTATCTCAATTTCTGTATATTCCCTGGGACTACGCACAAAAGCAGGCAGCAGTTAGAACATCTTTACAGAATGACAGCATTTGCACACCATCAATCAGTGTAGCAGATGCTGAGACTTTTGAGTCTCATATGATAGATTTCCTTAAAGGACAGATCAGCTCATATTTTACAGATAAACTTCTGGGAATTGTTCATGATACGATACTTCATATGAAGGAACTGAAAAAATCAGAGAATGACCATGATACATCTCTTGGGCTGCCACAAATTGCCGGTAATATCCACCAAGGGGATGCAGCACTGGAGTTCATTAAGCACGTCTGCGGTGTTTTCTCCATTGACCAAAATGTTATCCATGATGTTCTGGTAATGAGAAAGAATCTGTTGAAGATTGTACGTGTGAGGGAGTTTGCCCCTGAAGCAGAGTTTCGTGATCCCGGCGCAACCTTCACTTTACCAAATGTCATTTGCAGCTACTGCAACGACTGTAGAGACCTGGACTTGTGTCGCGACTCAGCTCTTCTAGCCGGGGAGTGGCGTTGTTGTGTACCACAATGTGGGCAGCTTTATGACAAAGAGGTGATGGAGAACGCCCTTCTTCAGATTGTGCGGCAGAGAGAGCGTCTCTACCAACTACAGGATCTCCAATGCCTCAGGTGTCACCAAGTTAAAGCTGCACGTTTAGCAGAGCAATGTGTATGCGCTGGCTCATATAAGTGCAGACAAAACGCAACTGAATTTCATACCCAAATGGAGTTAATTTGGAAGATTGCCATTCATCAGAAATTTCAACTTCTCCAAGAGTGCATTGAATGGAGTTTAGAAATGAATAACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009987 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

1989

Amino Acids

227.73

Weight (kDa)

6.37

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_B_exo1 PF03104 9 - 170 4.4e-47 DNA polymerase family B, exonuclease domain
DNA_pol_B PF00136 523 - 840 1.4e-17 DNA polymerase family B
POL2_thumb PF22634 715 - 890 1.3e-76 DNA polymerase epsilon catalytic subunit A, thumb domain
DUF1744 PF08490 1308 - 1672 1.4e-114 Domain of unknown function (DUF1744)
zf_DPOE_2 PF23250 1849 - 1915 2.6e-19 Zinc finger domain of DNA polymerase-epsilon
zf-DPOE PF22912 1920 - 1977 1.1e-12 Zinc finger domain of DNA polymerase-epsilon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3063, 3935
AasI GACNNNNNNGTC 2 cut(s) 1436, 5248
AatII GACGTC 1 cut(s) 2398
Acc16I TGCGCA 2 cut(s) 710, 1688
Acc65I GGTACC 1 cut(s) 3586
AccB1I GGYRCC 2 cut(s) 3586, 4820
AccB7I CCANNNNNTGG 2 cut(s) 2256, 5684
AccI GTMKAC 2 cut(s) 1657, 2352
AccII CGCG 3 cut(s) 356, 4838, 5637
AccIII TCCGGA 2 cut(s) 694, 4016
AciI CCGC 7 cut(s) 280, 354, 536, 4756, 4810, 5451, 5737
AclWI GGATC 8 cut(s) 791, 1843, 1958, 2799, 3548, 4736, 5555, 5775
AcoI YGGCCR 1 cut(s) 850
AcuI CTGAAG 9 cut(s) 48, 1130, 1464, 1587, 2177, 3348, 3786, 5565, 5711
AcyI GRCGYC 2 cut(s) 915, 2395
AdeI CACNNNGTG 2 cut(s) 4310, 5684
AfaI GTAC 7 cut(s) 191, 1529, 3588, 4001, 4560, 5523, 5676
AfeI AGCGCT 1 cut(s) 4181
AflII CTTAAG 1 cut(s) 500
AflIII ACRYGT 3 cut(s) 1211, 4576, 5524
AhlI ACTAGT 2 cut(s) 899, 3280
AjiI CACGTC 1 cut(s) 5446
AjuI GAANNNNNNNTTGG 2 cut(s) 4817, 4849
AleI CACNNNNGTG 1 cut(s) 4198
Alw21I GWGCWC 5 cut(s) 1828, 3761, 4081, 4306, 4609
Alw26I GTCTC 9 cut(s) 50, 696, 830, 1863, 2948, 5234, 5256, 5613, 5756
Alw44I GTGCAC 1 cut(s) 1824
AlwI GGATC 8 cut(s) 791, 1843, 1958, 2799, 3548, 4736, 5555, 5775
AlwNI CAGNNNCTG 6 cut(s) 89, 1442, 1967, 2528, 5237, 5604
Ama87I CYCGRG 2 cut(s) 1796, 4552
Aor13HI TCCGGA 2 cut(s) 694, 4016
Aor51HI AGCGCT 1 cut(s) 4181
AoxI GGCC 4 cut(s) 332, 496, 594, 850
ApaLI GTGCAC 1 cut(s) 1824
ArsI GACNNNNNNTTYG 2 cut(s) 2898, 2930
AseI ATTAAT 3 cut(s) 3378, 3993, 4368
Asp700I GAANNNNTTC 2 cut(s) 738, 2067
Asp718I GGTACC 1 cut(s) 3586
AspA2I CCTAGG 2 cut(s) 2690, 3917
AspLEI GCGC 6 cut(s) 711, 1689, 2757, 4182, 5570, 5831
AspS9I GGNCC 9 cut(s) 496, 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
AsuC2I CCSGG 2 cut(s) 5565, 5656
AsuII TTCGAA 1 cut(s) 4257
AvaI CYCGRG 2 cut(s) 1796, 4552
AvaII GGWCC 8 cut(s) 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
AvrII CCTAGG 2 cut(s) 2690, 3917
AxyI CCTNAGG 2 cut(s) 552, 5780
BaeGI GKGCMC 3 cut(s) 1085, 1828, 2480
BaeI ACNNNNGTAYC 4 cut(s) 2409, 2442, 3570, 3603
BalI TGGCCA 1 cut(s) 852
BanI GGYRCC 2 cut(s) 3586, 4820
BanII GRGCYC 1 cut(s) 4081
BauI CACGAG 1 cut(s) 4119
BbsI GAAGAC 3 cut(s) 1208, 2874, 3704
Bbv12I GWGCWC 5 cut(s) 1828, 3761, 4081, 4306, 4609
BceAI ACGGC 1 cut(s) 602
BcgI CGANNNNNNTGC 2 cut(s) 4698, 4732
BciVI GTATCC 2 cut(s) 1015, 1128
BclI TGATCA 2 cut(s) 936, 4312
BcnI CCSGG 2 cut(s) 5565, 5656
BcoDI GTCTC 9 cut(s) 50, 696, 830, 1863, 2948, 5234, 5256, 5613, 5756
BcuI ACTAGT 2 cut(s) 899, 3280
BfmI CTRYAG 2 cut(s) 5614, 5762
BfoI RGCGCY 1 cut(s) 4183
BfrI CTTAAG 1 cut(s) 500
BfuI GTATCC 2 cut(s) 1015, 1128
BglII AGATCT 3 cut(s) 10, 955, 4354
BlnI CCTAGG 2 cut(s) 2690, 3917
BlpI GCTNAGC 1 cut(s) 2181
Bme18I GGWCC 8 cut(s) 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
BmeT110I CYCGRG 2 cut(s) 1796, 4552
BmgBI CACGTC 1 cut(s) 5446
BmgT120I GGNCC 9 cut(s) 496, 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
BmiI GGNNCC 6 cut(s) 1744, 2110, 2111, 2902, 3588, 4822
BmrI ACTGGG 1 cut(s) 4720
BmuI ACTGGG 1 cut(s) 4720
BoxI GACNNNNGTC 1 cut(s) 5631
BpiI GAAGAC 3 cut(s) 1208, 2874, 3704
BplI GAGNNNNNCTC 2 cut(s) 5736, 5768
BpmI CTGGAG 5 cut(s) 560, 1743, 3417, 4446, 5450
Bpu1102I GCTNAGC 1 cut(s) 2181
Bpu14I TTCGAA 1 cut(s) 4257
BpuEI CTTGAG 3 cut(s) 468, 1367, 4210
BpuMI CCSGG 2 cut(s) 5565, 5656
Bsa29I ATCGAT 1 cut(s) 4708
BsaAI YACGTR 4 cut(s) 1073, 2548, 4310, 5525
BsaBI GATNNNNATC 3 cut(s) 474, 954, 4537
BsaHI GRCGYC 2 cut(s) 915, 2395
BsaI GGTCTC 1 cut(s) 5613
BsaWI WCCGGW 4 cut(s) 694, 1739, 4016, 5017
BsaXI ACNNNNNCTCC 2 cut(s) 5754, 5784
Bse118I RCCGGY 1 cut(s) 5399
Bse1I ACTGG 9 cut(s) 335, 1436, 2504, 2606, 3727, 4271, 4726, 4996, 5433
Bse21I CCTNAGG 2 cut(s) 552, 5780
Bse3DI GCAATG 4 cut(s) 1119, 2043, 2670, 5825
Bse8I GATNNNNATC 3 cut(s) 474, 954, 4537
BseAI TCCGGA 2 cut(s) 694, 4016
BseCI ATCGAT 1 cut(s) 4708
BseJI GATNNNNATC 3 cut(s) 474, 954, 4537
BseMI GCAATG 4 cut(s) 1119, 2043, 2670, 5825
BseNI ACTGG 9 cut(s) 335, 1436, 2504, 2606, 3727, 4271, 4726, 4996, 5433
BseSI GKGCMC 3 cut(s) 1085, 1828, 2480
BsgI GTGCAG 4 cut(s) 2397, 3674, 5787, 5866
Bsh1236I CGCG 3 cut(s) 356, 4838, 5637
BshFI GGCC 4 cut(s) 334, 498, 596, 852
BshNI GGYRCC 2 cut(s) 3586, 4820
BshVI ATCGAT 1 cut(s) 4708
BsiHKAI GWGCWC 5 cut(s) 1828, 3761, 4081, 4306, 4609
BsiHKCI CYCGRG 2 cut(s) 1796, 4552
BsiSI CCGG 8 cut(s) 695, 1740, 2657, 4017, 5018, 5400, 5565, 5655
BslFI GGGAC 4 cut(s) 2122, 3014, 4707, 5168
BsmAI GTCTC 9 cut(s) 50, 696, 830, 1863, 2948, 5234, 5256, 5613, 5756
BsmBI CGTCTC 2 cut(s) 50, 5756
BsmFI GGGAC 4 cut(s) 2122, 3014, 4707, 5168
BsnI GGCC 4 cut(s) 334, 498, 596, 852
Bso31I GGTCTC 1 cut(s) 5613
BsoBI CYCGRG 2 cut(s) 1796, 4552
Bsp119I TTCGAA 1 cut(s) 4257
Bsp1286I GDGCHC 7 cut(s) 1085, 1828, 2480, 3761, 4081, 4306, 4609
Bsp13I TCCGGA 2 cut(s) 694, 4016
Bsp1720I GCTNAGC 1 cut(s) 2181
Bsp19I CCATGG 1 cut(s) 1710
Bsp68I TCGCGA 2 cut(s) 4838, 5637
BspACI CCGC 7 cut(s) 280, 354, 536, 4756, 4810, 5451, 5737
BspANI GGCC 4 cut(s) 334, 498, 596, 852
BspDI ATCGAT 1 cut(s) 4708
BspEI TCCGGA 2 cut(s) 694, 4016
BspFNI CGCG 3 cut(s) 356, 4838, 5637
BspHI TCATGA 4 cut(s) 3336, 4738, 4773, 5320
BspLI GGNNCC 6 cut(s) 1744, 2110, 2111, 2902, 3588, 4822
BspPI GGATC 8 cut(s) 791, 1843, 1958, 2799, 3548, 4736, 5555, 5775
BspQI GCTCTTC 4 cut(s) 3462, 3549, 4755, 5652
BspT104I TTCGAA 1 cut(s) 4257
BspT107I GGYRCC 2 cut(s) 3586, 4820
BspTI CTTAAG 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 5613
BsrDI GCAATG 4 cut(s) 1119, 2043, 2670, 5825
BsrFI RCCGGY 1 cut(s) 5399
BsrI ACTGG 9 cut(s) 335, 1436, 2504, 2606, 3727, 4271, 4726, 4996, 5433
BssAI RCCGGY 1 cut(s) 5399
BssNAI GTATAC 1 cut(s) 1658
BssNI GRCGYC 2 cut(s) 915, 2395
BssSI CACGAG 1 cut(s) 4119
BssT1I CCWWGG 6 cut(s) 1572, 1710, 2623, 2690, 3917, 5413
Bst1107I GTATAC 1 cut(s) 1658
Bst2BI CACGAG 1 cut(s) 4119
Bst6I CTCTTC 8 cut(s) 1019, 1071, 3080, 3462, 3549, 3647, 4755, 5652
BstACI GRCGYC 2 cut(s) 915, 2395
BstAFI CTTAAG 1 cut(s) 500
BstAPI GCANNNNNTGC 2 cut(s) 3146, 5604
BstBAI YACGTR 4 cut(s) 1073, 2548, 4310, 5525
BstBI TTCGAA 1 cut(s) 4257
BstDSI CCRYGG 2 cut(s) 1710, 4446
BstEII GGTNACC 2 cut(s) 3299, 4441
BstENI CCTNNNNNAGG 1 cut(s) 3488
BstFNI CGCG 3 cut(s) 356, 4838, 5637
BstH2I RGCGCY 1 cut(s) 4183
BstHHI GCGC 6 cut(s) 711, 1689, 2757, 4182, 5570, 5831
BstMAI GTCTC 9 cut(s) 50, 696, 830, 1863, 2948, 5234, 5256, 5613, 5756
BstNSI RCATGY 4 cut(s) 1215, 2466, 3891, 4580
BstPAI GACNNNNGTC 1 cut(s) 5631
BstPI GGTNACC 2 cut(s) 3299, 4441
BstSFI CTRYAG 2 cut(s) 5614, 5762
BstSLI GKGCMC 3 cut(s) 1085, 1828, 2480
BstSNI TACGTA 1 cut(s) 2548
BstUI CGCG 3 cut(s) 356, 4838, 5637
BstV2I GAAGAC 3 cut(s) 1208, 2874, 3704
BstX2I RGATCY 6 cut(s) 10, 955, 1835, 1963, 4354, 5767
BstXI CCANNNNNNTGG 2 cut(s) 1717, 4142
BstYI RGATCY 6 cut(s) 10, 955, 1835, 1963, 4354, 5767
BstZ17I GTATAC 1 cut(s) 1658
Bsu15I ATCGAT 1 cut(s) 4708
Bsu36I CCTNAGG 2 cut(s) 552, 5780
BsuI GTATCC 2 cut(s) 1015, 1128
BsuRI GGCC 4 cut(s) 334, 498, 596, 852
BsuTUI ATCGAT 1 cut(s) 4708
BtgI CCRYGG 2 cut(s) 1710, 4446
BtrI CACGTC 1 cut(s) 5446
BtsI GCAGTG 4 cut(s) 1179, 2385, 2385, 2818
BtsIMutI CAGTG 9 cut(s) 1179, 1440, 2305, 2385, 2385, 2818, 4205, 5230, 5426
BtuMI TCGCGA 2 cut(s) 4838, 5637
CaiI CAGNNNCTG 6 cut(s) 89, 1442, 1967, 2528, 5237, 5604
CciI TCATGA 4 cut(s) 3336, 4738, 4773, 5320
CfoI GCGC 6 cut(s) 711, 1689, 2757, 4182, 5570, 5831
Cfr10I RCCGGY 1 cut(s) 5399
Cfr13I GGNCC 9 cut(s) 496, 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
ClaI ATCGAT 1 cut(s) 4708
CseI GACGC 3 cut(s) 923, 1376, 5738
CsiI ACCWGGT 2 cut(s) 4134, 4497
Csp6I GTAC 7 cut(s) 190, 1528, 3587, 4000, 4559, 5522, 5675
CspCI CAANNNNNGTGG 4 cut(s) 700, 735, 1561, 1596
CviQI GTAC 7 cut(s) 190, 1528, 3587, 4000, 4559, 5522, 5675
DraIII CACNNNGTG 2 cut(s) 4310, 5684
DrdI GACNNNNNNGTC 2 cut(s) 1436, 5248
DseDI GACNNNNNNGTC 2 cut(s) 1436, 5248
EaeI YGGCCR 1 cut(s) 850
Eam1104I CTCTTC 8 cut(s) 1019, 1071, 3080, 3462, 3549, 3647, 4755, 5652
EarI CTCTTC 8 cut(s) 1019, 1071, 3080, 3462, 3549, 3647, 4755, 5652
Ecl136II GAGCTC 1 cut(s) 4079
Eco105I TACGTA 1 cut(s) 2548
Eco130I CCWWGG 6 cut(s) 1572, 1710, 2623, 2690, 3917, 5413
Eco24I GRGCYC 1 cut(s) 4081
Eco31I GGTCTC 1 cut(s) 5613
Eco32I GATATC 2 cut(s) 2770, 4335
Eco47I GGWCC 8 cut(s) 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
Eco47III AGCGCT 1 cut(s) 4181
Eco53kI GAGCTC 1 cut(s) 4079
Eco57I CTGAAG 9 cut(s) 48, 1130, 1464, 1587, 2177, 3348, 3786, 5565, 5711
Eco81I CCTNAGG 2 cut(s) 552, 5780
Eco88I CYCGRG 2 cut(s) 1796, 4552
Eco91I GGTNACC 2 cut(s) 3299, 4441
EcoICRI GAGCTC 1 cut(s) 4079
EcoNI CCTNNNNNAGG 1 cut(s) 3488
EcoO109I RGGNCCY 2 cut(s) 496, 2109
EcoO65I GGTNACC 2 cut(s) 3299, 4441
EcoRI GAATTC 2 cut(s) 3220, 5013
EcoRV GATATC 2 cut(s) 2770, 4335
EcoT14I CCWWGG 6 cut(s) 1572, 1710, 2623, 2690, 3917, 5413
EcoT22I ATGCAT 4 cut(s) 1469, 3044, 3889, 4690
EcoT38I GRGCYC 1 cut(s) 4081
ErhI CCWWGG 6 cut(s) 1572, 1710, 2623, 2690, 3917, 5413
Esp3I CGTCTC 2 cut(s) 50, 5756
FalI AAGNNNNNCTT 6 cut(s) 977, 1009, 1374, 1406, 3090, 3122
FaqI GGGAC 4 cut(s) 2122, 3014, 4707, 5168
FauI CCCGC 1 cut(s) 273
FauNDI CATATG 3 cut(s) 276, 5256, 5337
FbaI TGATCA 2 cut(s) 936, 4312
FblI GTMKAC 2 cut(s) 1657, 2352
FriOI GRGCYC 1 cut(s) 4081
FspI TGCGCA 2 cut(s) 710, 1688
GlaI GCGC 6 cut(s) 710, 1688, 2756, 4181, 5569, 5830
GsuI CTGGAG 5 cut(s) 560, 1743, 3417, 4446, 5450
HaeII RGCGCY 1 cut(s) 4183
HaeIII GGCC 4 cut(s) 334, 498, 596, 852
HapII CCGG 8 cut(s) 695, 1740, 2657, 4017, 5018, 5400, 5565, 5655
HgaI GACGC 3 cut(s) 923, 1376, 5738
HhaI GCGC 6 cut(s) 711, 1689, 2757, 4182, 5570, 5831
Hin1I GRCGYC 2 cut(s) 915, 2395
Hin6I GCGC 6 cut(s) 709, 1687, 2755, 4180, 5568, 5829
HinP1I GCGC 6 cut(s) 709, 1687, 2755, 4180, 5568, 5829
HincII GTYRAC 1 cut(s) 1312
HindII GTYRAC 1 cut(s) 1312
HindIII AAGCTT 7 cut(s) 908, 1043, 1223, 3533, 4593, 4733, 4893
HpaII CCGG 8 cut(s) 695, 1740, 2657, 4017, 5018, 5400, 5565, 5655
Hpy99I CGWCG 1 cut(s) 64
HpyCH4IV ACGT 9 cut(s) 466, 1072, 2395, 2547, 3660, 4309, 5445, 5524, 5806
HpySE526I ACGT 9 cut(s) 466, 1072, 2395, 2547, 3660, 4309, 5445, 5524, 5806
Hsp92I GRCGYC 2 cut(s) 915, 2395
HspAI GCGC 6 cut(s) 709, 1687, 2755, 4180, 5568, 5829
KflI GGGWCCC 1 cut(s) 2109
Kpn2I TCCGGA 2 cut(s) 694, 4016
KpnI GGTACC 1 cut(s) 3590
Ksp22I TGATCA 2 cut(s) 936, 4312
LguI GCTCTTC 4 cut(s) 3462, 3549, 4755, 5652
LmnI GCTCC 4 cut(s) 1742, 4076, 4427, 4612
MabI ACCWGGT 2 cut(s) 4134, 4497
MaeII ACGT 9 cut(s) 466, 1072, 2395, 2547, 3660, 4309, 5445, 5524, 5806
MfeI CAATTG 6 cut(s) 932, 2004, 2910, 3498, 4026, 4055
MflI RGATCY 6 cut(s) 10, 955, 1835, 1963, 4354, 5767
MhlI GDGCHC 7 cut(s) 1085, 1828, 2480, 3761, 4081, 4306, 4609
MlsI TGGCCA 1 cut(s) 852
MluNI TGGCCA 1 cut(s) 852
MlyI GAGTC 8 cut(s) 1307, 1948, 1981, 2333, 2819, 4629, 5258, 5633
MmeI TCCRAC 4 cut(s) 367, 920, 3653, 3931
Mox20I TGGCCA 1 cut(s) 852
Mph1103I ATGCAT 4 cut(s) 1469, 3044, 3889, 4690
MroI TCCGGA 2 cut(s) 694, 4016
MroXI GAANNNNTTC 2 cut(s) 738, 2067
MscI TGGCCA 1 cut(s) 852
MslI CAYNNNNRTG 9 cut(s) 39, 1101, 1651, 1715, 1897, 3243, 3486, 4198, 5842
Msp20I TGGCCA 1 cut(s) 852
MspA1I CMGCKG 2 cut(s) 538, 4886
MspCI CTTAAG 1 cut(s) 500
MspI CCGG 8 cut(s) 695, 1740, 2657, 4017, 5018, 5400, 5565, 5655
MunI CAATTG 6 cut(s) 932, 2004, 2910, 3498, 4026, 4055
MvnI CGCG 3 cut(s) 356, 4838, 5637
NciI CCSGG 2 cut(s) 5565, 5656
NcoI CCATGG 1 cut(s) 1710
NdeI CATATG 3 cut(s) 276, 5256, 5337
NlaIV GGNNCC 6 cut(s) 1744, 2110, 2111, 2902, 3588, 4822
NmeAIII GCCGAG 1 cut(s) 446
NruI TCGCGA 2 cut(s) 4838, 5637
NsbI TGCGCA 2 cut(s) 710, 1688
NsiI ATGCAT 4 cut(s) 1469, 3044, 3889, 4690
NspI RCATGY 4 cut(s) 1215, 2466, 3891, 4580
NspV TTCGAA 1 cut(s) 4257
OliI CACNNNNGTG 1 cut(s) 4198
PaeI GCATGC 1 cut(s) 3891
PaeR7I CTCGAG 1 cut(s) 1796
PagI TCATGA 4 cut(s) 3336, 4738, 4773, 5320
PasI CCCWGGG 1 cut(s) 5151
PciI ACATGT 2 cut(s) 1211, 4576
PciSI GCTCTTC 4 cut(s) 3462, 3549, 4755, 5652
PcsI WCGNNNNNNNCGW 1 cut(s) 1507
PdmI GAANNNNTTC 2 cut(s) 738, 2067
PflMI CCANNNNNTGG 2 cut(s) 2256, 5684
PfoI TCCNGGA 3 cut(s) 339, 1599, 3433
PleI GAGTC 8 cut(s) 1306, 1948, 1981, 2332, 2819, 4628, 5257, 5633
PpsI GAGTC 8 cut(s) 1306, 1948, 1981, 2332, 2819, 4628, 5257, 5633
Ppu21I YACGTR 4 cut(s) 1073, 2548, 4310, 5525
PpuMI RGGWCCY 1 cut(s) 2109
PscI ACATGT 2 cut(s) 1211, 4576
PshAI GACNNNNGTC 1 cut(s) 5631
PshBI ATTAAT 3 cut(s) 3378, 3993, 4368
PsiI TTATAA 2 cut(s) 3063, 3935
Psp124BI GAGCTC 1 cut(s) 4081
Psp5II RGGWCCY 1 cut(s) 2109
PspEI GGTNACC 2 cut(s) 3299, 4441
PspN4I GGNNCC 6 cut(s) 1744, 2110, 2111, 2902, 3588, 4822
PspPI GGNCC 9 cut(s) 496, 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
PspPPI RGGWCCY 1 cut(s) 2109
PsrI GAACNNNNNNTAC 2 cut(s) 350, 382
PstNI CAGNNNCTG 6 cut(s) 89, 1442, 1967, 2528, 5237, 5604
PsuI RGATCY 6 cut(s) 10, 955, 1835, 1963, 4354, 5767
PvuII CAGCTG 1 cut(s) 4886
RruI TCGCGA 2 cut(s) 4838, 5637
RsaI GTAC 7 cut(s) 191, 1529, 3588, 4001, 4560, 5523, 5676
RsaNI GTAC 7 cut(s) 190, 1528, 3587, 4000, 4559, 5522, 5675
RseI CAYNNNNRTG 9 cut(s) 39, 1101, 1651, 1715, 1897, 3243, 3486, 4198, 5842
SacI GAGCTC 1 cut(s) 4081
SapI GCTCTTC 4 cut(s) 3462, 3549, 4755, 5652
Sau96I GGNCC 9 cut(s) 496, 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
SchI GAGTC 8 cut(s) 1307, 1948, 1981, 2333, 2819, 4629, 5258, 5633
SduI GDGCHC 7 cut(s) 1085, 1828, 2480, 3761, 4081, 4306, 4609
SexAI ACCWGGT 2 cut(s) 4134, 4497
SfcI CTRYAG 2 cut(s) 5614, 5762
Sfr274I CTCGAG 1 cut(s) 1796
SfuI TTCGAA 1 cut(s) 4257
SinI GGWCC 8 cut(s) 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
SlaI CTCGAG 1 cut(s) 1796
SmiMI CAYNNNNRTG 9 cut(s) 39, 1101, 1651, 1715, 1897, 3243, 3486, 4198, 5842
SmlI CTYRAG 5 cut(s) 483, 500, 1346, 1796, 4225
SmoI CTYRAG 5 cut(s) 483, 500, 1346, 1796, 4225
SnaBI TACGTA 1 cut(s) 2548
SpeI ACTAGT 2 cut(s) 899, 3280
SphI GCATGC 1 cut(s) 3891
SsiI CCGC 7 cut(s) 280, 354, 536, 4756, 4810, 5451, 5737
SspI AATATT 5 cut(s) 1251, 2514, 2945, 4833, 5080
SstI GAGCTC 1 cut(s) 4081
StyI CCWWGG 6 cut(s) 1572, 1710, 2623, 2690, 3917, 5413
TaiI ACGT 9 cut(s) 469, 1075, 2398, 2550, 3663, 4312, 5448, 5527, 5809
TatI WGTACW 4 cut(s) 189, 1527, 3999, 4558
TauI GCSGC 2 cut(s) 356, 5740
TscAI CASTG 9 cut(s) 1186, 1447, 2305, 2385, 2392, 2818, 4205, 5230, 5433
TspGWI ACGGA 2 cut(s) 3722, 3869
TspRI CASTG 9 cut(s) 1186, 1447, 2305, 2385, 2392, 2818, 4205, 5230, 5433
Van91I CCANNNNNTGG 2 cut(s) 2256, 5684
Vha464I CTTAAG 1 cut(s) 500
VneI GTGCAC 1 cut(s) 1824
VpaK11BI GGWCC 8 cut(s) 748, 2024, 2109, 2604, 2900, 4019, 4993, 5110
VspI ATTAAT 3 cut(s) 3378, 3993, 4368
XagI CCTNNNNNAGG 1 cut(s) 3488
XceI RCATGY 4 cut(s) 1215, 2466, 3891, 4580
XcmI CCANNNNNNNNNTGG 2 cut(s) 4968, 5003
XhoI CTCGAG 1 cut(s) 1796
XmaJI CCTAGG 2 cut(s) 2690, 3917
XmiI GTMKAC 2 cut(s) 1657, 2352
XmnI GAANNNNTTC 2 cut(s) 738, 2067
ZraI GACGTC 1 cut(s) 2396
Zsp2I ATGCAT 4 cut(s) 1469, 3044, 3889, 4690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.