pycom11g21760

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006497
Physical Location & Seq
Forward (+)
24808951 .. 24810407
1457 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1014 bp
ATGGTAGAGCTAAACTCCAAAGCCAACGCTGAAAGGAACCGCATTCACCCCGGAGTCCTCGACCCATGCAAAAGACCAGGAGGGCCACATCCTGGTTGTAACGGTGACGTCAAAAGTCCACCCCAACCCGGGAACCCCTATAACCACGGTTGCTCAAAGATTTATCGGTGTCGCGGTGGTCATGATAAGGTTGATACTCACAAGGTGAGGGAAAACATTTATATATTTATGTTGCAACTATTACCTACTTTGTTTCTATTCTATAGTGAGAATAAGGGTGATAAATCTTTTAGGCAAAGTATTTTAATTTTTTACATTGCTAGTAATATTTTTACTTATAAATTATTTATTAGGCTAATATCTAATTACTTAGTCCAATTGTTATATATTGAGACAACACTTTTTAAACATTTTAAGAAAGGACATTTTTTAAACCCGTGTAAGATGATGTCATGTCCGGTTTTTCCTGATTTGAATCATTCAAATTATATTTTTGTTTCTTTGCTGTTTTTCTTTCTCTCTGAAGTTTATCTGTTCCGATTTATATTTCGTCATCTCCCTCAATTATCTCTCGCCATAATTTGTATTGGAATCATTCAAGTTTTCTTCGAAGGTAAGTGTTTCTCTCAAGTTTATCTCTCTGAATTTATATGTTCCGATTTAGATTTTCAAAGTGTTTGTCTGATTTTTCAGTTTTTTATTGGTCGCATTGAGTTTTTTTGGATTGTTCTTGTTTTTTGCAGTCACGGTTTTTTAGTTTTGAAGATTTTGATTCGGCTTTTGTTTTTCATTAAAGTTCCTTTTGGTTTTTGCAGTGTATTTAGCATTTGGCGAAAGTTATTAATATTTATGCAGATCAGGGTGGGGGAGGTTGGGGGGGACCCCCCACATAAACATTTAATAATTTTCAGCGAAATACTTGTGCATCATCAGTGTACTTGTGCAATATCAGCTCATAGAATGTCATTCTGTGATAAATCCTTGGTCAATAAAAGCATATTTGATAGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

338

Amino Acids

39.15

Weight (kDa)

9.11

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 9 - 59 1.4e-07 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AatII GACGTC 1 cut(s) 111
AccB7I CCANNNNNTGG 1 cut(s) 92
AccII CGCG 1 cut(s) 174
AciI CCGC 2 cut(s) 40, 174
AcsI RAATTY 1 cut(s) 644
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 205
AfaI GTAC 1 cut(s) 937
AfiI CCNNNNNNNGG 3 cut(s) 92, 128, 129
AgsI TTSAA 5 cut(s) 475, 483, 599, 671, 763
AjnI CCWGG 2 cut(s) 76, 91
AluBI AGCT 2 cut(s) 10, 953
AluI AGCT 2 cut(s) 10, 953
Alw26I GTCTC 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 644
ArsI GACNNNNNNTTYG 2 cut(s) 664, 696
AseI ATTAAT 1 cut(s) 842
AspS9I GGNCC 2 cut(s) 83, 880
AsuC2I CCSGG 3 cut(s) 51, 129, 130
AsuHPI GGTGA 4 cut(s) 38, 116, 217, 290
AsuII TTCGAA 1 cut(s) 609
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 880
BciT130I CCWGG 2 cut(s) 78, 93
BcnI CCSGG 3 cut(s) 51, 129, 130
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 1 cut(s) 262
Bme1390I CCNGG 5 cut(s) 51, 78, 93, 129, 130
Bme18I GGWCC 1 cut(s) 880
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 83, 880
BmiI GGNNCC 4 cut(s) 38, 134, 881, 882
BmrFI CCNGG 5 cut(s) 51, 78, 93, 129, 130
BmsI GCATC 1 cut(s) 934
BplI GAGNNNNNCTC 1 cut(s) 31
Bpu14I TTCGAA 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 612
BpuMI CCSGG 3 cut(s) 51, 129, 130
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 4 cut(s) 49, 128, 145, 981
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 128, 129
Bse3DI GCAATG 1 cut(s) 315
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 2 cut(s) 78, 93
BseDI CCNNGG 4 cut(s) 49, 128, 145, 981
BseGI GGATG 1 cut(s) 88
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 3 cut(s) 92, 128, 129
BseMI GCAATG 1 cut(s) 315
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 85
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 3 cut(s) 51, 129, 458
BslFI GGGAC 1 cut(s) 893
BslI CCNNNNNNNGG 3 cut(s) 92, 128, 129
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 893
BsmI GAATGC 1 cut(s) 42
BsnI GGCC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 128
Bsp119I TTCGAA 1 cut(s) 609
Bsp143I GATC 1 cut(s) 855
BspACI CCGC 2 cut(s) 40, 174
BspANI GGCC 1 cut(s) 85
BspFNI CGCG 1 cut(s) 174
BspHI TCATGA 2 cut(s) 181, 1010
BspLI GGNNCC 4 cut(s) 38, 134, 881, 882
BspT104I TTCGAA 1 cut(s) 609
BsrDI GCAATG 1 cut(s) 315
BssECI CCNNGG 4 cut(s) 49, 128, 145, 981
BssMI GATC 1 cut(s) 855
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 981
Bst2UI CCWGG 2 cut(s) 78, 93
Bst4CI ACNGT 3 cut(s) 104, 149, 749
BstACI GRCGYC 1 cut(s) 108
BstBI TTCGAA 1 cut(s) 609
BstDEI CTNAG 1 cut(s) 370
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 1 cut(s) 88
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 1 cut(s) 858
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 1 cut(s) 855
BstMWI GCNNNNNNNGC 1 cut(s) 950
BstNI CCWGG 2 cut(s) 78, 93
BstSCI CCNGG 5 cut(s) 49, 76, 91, 127, 128
BstSFI CTRYAG 1 cut(s) 262
BstUI CGCG 1 cut(s) 174
BsuRI GGCC 1 cut(s) 85
BtgI CCRYGG 1 cut(s) 145
BtsCI GGATG 1 cut(s) 88
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 2 cut(s) 820, 938
CciI TCATGA 2 cut(s) 181, 1010
Cfr13I GGNCC 2 cut(s) 83, 880
Cfr9I CCCGGG 1 cut(s) 128
Csp6I GTAC 1 cut(s) 936
CviAII CATG 4 cut(s) 66, 182, 453, 1011
CviJI RGCY 6 cut(s) 10, 23, 85, 355, 778, 953
CviKI_1 RGCY 6 cut(s) 10, 23, 85, 355, 778, 953
CviQI GTAC 1 cut(s) 936
DdeI CTNAG 1 cut(s) 370
DpnI GATC 1 cut(s) 857
DpnII GATC 1 cut(s) 855
DraI TTTAAA 2 cut(s) 406, 432
DraIII CACNNNGTG 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 981
Eco47I GGWCC 1 cut(s) 880
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 880
EcoRII CCWGG 2 cut(s) 76, 91
EcoT14I CCWWGG 1 cut(s) 981
ErhI CCWWGG 1 cut(s) 981
FaeI CATG 4 cut(s) 69, 185, 456, 1014
FaqI GGGAC 1 cut(s) 893
FatI CATG 4 cut(s) 65, 181, 452, 1010
FokI GGATG 1 cut(s) 75
FspBI CTAG 1 cut(s) 321
HaeIII GGCC 1 cut(s) 85
HapII CCGG 3 cut(s) 51, 129, 458
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 4 cut(s) 69, 185, 456, 1014
HinfI GANTC 4 cut(s) 54, 475, 591, 772
HpaII CCGG 3 cut(s) 51, 129, 458
HphI GGTGA 4 cut(s) 38, 116, 217, 290
Hpy166II GTNNAC 2 cut(s) 119, 936
Hpy188I TCNGA 5 cut(s) 523, 539, 643, 658, 684
Hpy188III TCNNGA 3 cut(s) 182, 467, 1011
Hpy8I GTNNAC 2 cut(s) 119, 936
HpyAV CCTTC 1 cut(s) 605
HpyCH4III ACNGT 3 cut(s) 104, 149, 749
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 7 cut(s) 69, 235, 741, 813, 853, 925, 944
HpyF10VI GCNNNNNNNGC 1 cut(s) 950
HpyF3I CTNAG 1 cut(s) 370
HpySE526I ACGT 1 cut(s) 108
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 4 cut(s) 69, 185, 456, 1014
KflI GGGWCCC 1 cut(s) 880
Kzo9I GATC 1 cut(s) 855
LpnPI CCDG 9 cut(s) 63, 64, 78, 90, 105, 142, 471, 480, 844
LweI GCATC 1 cut(s) 934
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 3 cut(s) 98, 104, 743
MalI GATC 1 cut(s) 857
MboI GATC 1 cut(s) 855
MboII GAAGA 2 cut(s) 598, 775
MfeI CAATTG 1 cut(s) 377
MluCI AATT 9 cut(s) 306, 341, 364, 377, 484, 563, 579, 644, 903
MlyI GAGTC 1 cut(s) 63
MnlI CCTC 5 cut(s) 68, 74, 201, 570, 862
MseI TTAA 7 cut(s) 305, 405, 414, 431, 792, 842, 899
MspI CCGG 3 cut(s) 51, 129, 458
MspR9I CCNGG 5 cut(s) 51, 78, 93, 129, 130
MunI CAATTG 1 cut(s) 377
Mva1269I GAATGC 1 cut(s) 42
MvaI CCWGG 2 cut(s) 78, 93
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 950
NciI CCSGG 3 cut(s) 51, 129, 130
NdeII GATC 1 cut(s) 855
NlaIII CATG 4 cut(s) 69, 185, 456, 1014
NlaIV GGNNCC 4 cut(s) 38, 134, 881, 882
NmuCI GTSAC 2 cut(s) 104, 743
NspV TTCGAA 1 cut(s) 609
PagI TCATGA 2 cut(s) 181, 1010
PctI GAATGC 1 cut(s) 42
PfeI GAWTC 3 cut(s) 475, 591, 772
PflMI CCANNNNNTGG 1 cut(s) 92
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 880
PshBI ATTAAT 1 cut(s) 842
PsiI TTATAA 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 880
Psp6I CCWGG 2 cut(s) 76, 91
PspGI CCWGG 2 cut(s) 76, 91
PspN4I GGNNCC 4 cut(s) 38, 134, 881, 882
PspPI GGNCC 2 cut(s) 83, 880
PspPPI RGGWCCY 1 cut(s) 880
RsaI GTAC 1 cut(s) 937
RsaNI GTAC 1 cut(s) 936
SaqAI TTAA 7 cut(s) 305, 405, 414, 431, 792, 842, 899
Sau3AI GATC 1 cut(s) 855
Sau96I GGNCC 2 cut(s) 83, 880
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 5 cut(s) 51, 78, 93, 129, 130
SetI ASST 8 cut(s) 12, 111, 192, 207, 247, 616, 873, 955
SfaNI GCATC 1 cut(s) 934
SfcI CTRYAG 1 cut(s) 262
SfuI TTCGAA 1 cut(s) 609
SinI GGWCC 1 cut(s) 880
SmaI CCCGGG 1 cut(s) 130
SmlI CTYRAG 1 cut(s) 627
SmoI CTYRAG 1 cut(s) 627
Sse9I AATT 9 cut(s) 306, 341, 364, 377, 484, 563, 579, 644, 903
SsiI CCGC 2 cut(s) 40, 174
SspI AATATT 2 cut(s) 328, 846
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 5 cut(s) 49, 76, 91, 127, 128
StyI CCWWGG 1 cut(s) 981
TaaI ACNGT 3 cut(s) 104, 149, 749
TaiI ACGT 1 cut(s) 111
TaqI TCGA 2 cut(s) 60, 609
TasI AATT 9 cut(s) 306, 341, 364, 377, 484, 563, 579, 644, 903
TatI WGTACW 1 cut(s) 935
TfiI GAWTC 3 cut(s) 475, 591, 772
Tru1I TTAA 7 cut(s) 305, 405, 414, 431, 792, 842, 899
Tru9I TTAA 7 cut(s) 305, 405, 414, 431, 792, 842, 899
TscAI CASTG 2 cut(s) 820, 938
TseFI GTSAC 2 cut(s) 104, 743
Tsp45I GTSAC 2 cut(s) 104, 743
TspDTI ATGAA 2 cut(s) 778, 999
TspMI CCCGGG 1 cut(s) 128
TspRI CASTG 2 cut(s) 820, 938
Van91I CCANNNNNTGG 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 880
VspI ATTAAT 1 cut(s) 842
XapI RAATTY 1 cut(s) 644
XmaI CCCGGG 1 cut(s) 128
XspI CTAG 1 cut(s) 321
ZraI GACGTC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.