pycom11g23280

Alpha-1,3 1,6-mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0004054
Physical Location & Seq
Forward (+)
26292722 .. 26296243
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g23280.1

Sequence Viewer

Length: 1233 bp
ATGGCGGAGAAGAAAGGAGGCTCCAAGTTGAACGTTGCAATCATTCATCCCGATCTGGGCATAGGTGGAGCTGAAAGACTGATCGTCGACGCGGCTGTCGAACTTGCATCACATGGCCACAAAGTTCACGTCTTCACTTCCCACCACGATAAAAATCGATGCTTTGAGGAGACCGTTTCTGGTATCTTTCCTGTTACTGTATATGGATCTTTCCTACCCCGACATATATTCTATCGACTCCATGCTTTGTGTGCATATATACGGTGCCTTTTTGTTGCTCTCTGCATGCTGGTCATGTGGCCATCATTTGATGTAATACTAGCAGATCAGGTTTCTGTTGTCATCCCACTACTGAAGCTTAAGAAGGCAACAAAGGTTTTGTTTTACTGTCATTTTCCGGATTTATTGCTGGCTCAACACACAACTCTTTTGAGGAGGATATATAGAAAACCCATAGACTTCATAGAAGAAATGACAACTGGAATGGCACATAAGATTCTTGTTAACAGCAAATTTACAGCATCTATGTTTGCAAAGACATTTAAGCATCTTGCCGCTCAAGGGATTCAGCCAGCTGTTCTTTATCCGGCTGTCAATGTGAATCAGTTTAATGAACCCGCCAATTCTTACAAGCTAAATTTTCTGTCTATCAATCGTTTCGAGAGGAAAAAGAATATAGACTTGGCAATATCAGCTTTTGCTCTGCTTCGTACCCTTGAAGGGGATGTCCTTCAAGGTCCTGATCTGGCTGAAGCTTCCTTGACAATTGCAGGTGGCTTCGATAATCGTCTGAGAGAAAATGTTGAGTACTTAGAGGAGCTCAGAAGCCTAGCAGAAAGGGAAGGAGTGTCCAGTCAAGTTAACTTCATCACCTCTTGCTCAACAGCAGAAAGAAATGTACTTCTCTCCCAATGCCTATGCGTCCTTTATACGCCAAAGGATGAACACTTTGGCATTGTACCTCTGGAGGCAATGGCCGCTCATAAACCTGTTATTGCATGCAACAGTGGGGGCCCTGTAGAGACAGTTAAGAATGGTGAAACGGGATTTCTGTGTGACTGTAACCCAAGAGAGTTCTCCTTGGCTATGGCTAAACTCATAAAAGACCCGCAGATGGCTCAGAGAATGGGTATAGAAGCACGACGGCATGTCACAGAGTCATTCTCTACGAAAATATTTGGTCAGCACTTGAATCAATACATTGCTGATGTTGCTCGGACGAAAAGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko00513 ko01100 map00510 map00513 map01100
Pfam Domains
Protein Families
Glyco_transf_4 Glycos_transf_1

Protein Analysis

411

Amino Acids

45.81

Weight (kDa)

8.55

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_4 PF13439 21 - 180 3.9e-13 Glycosyltransferase Family 4
Glyco_trans_4_4 PF13579 22 - 175 7.6e-06 Glycosyl transferase 4-like domain
GT4-conflict PF20706 182 - 343 2.5e-08 Family 4 Glycosyltransferase in conflict systems
Glycos_transf_1 PF00534 205 - 382 4.6e-35 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 214 - 369 6.7e-19 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 761
AasI GACNNNNNNGTC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 761
AccB1I GGYRCC 1 cut(s) 264
AccBSI CCGCTC 2 cut(s) 557, 980
AccI GTMKAC 1 cut(s) 87
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 6 cut(s) 5, 92, 555, 618, 978, 1109
AclI AACGTT 1 cut(s) 33
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 3 cut(s) 115, 299, 975
AcsI RAATTY 2 cut(s) 512, 637
AcuI CTGAAG 2 cut(s) 374, 771
AfaI GTAC 4 cut(s) 712, 809, 900, 960
AfiI CCNNNNNNNGG 5 cut(s) 56, 561, 720, 721, 1114
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 4 cut(s) 31, 719, 734, 1192
AhdI GACNNNNNGTC 2 cut(s) 83, 1148
AjiI CACGTC 1 cut(s) 130
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
AluBI AGCT 7 cut(s) 71, 358, 575, 634, 695, 755, 820
AluI AGCT 7 cut(s) 71, 358, 575, 634, 695, 755, 820
Alw21I GWGCWC 1 cut(s) 822
Alw26I GTCTC 3 cut(s) 164, 1016, 1221
AlwI GGATC 1 cut(s) 214
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 4 cut(s) 115, 299, 975, 1012
ApaI GGGCCC 1 cut(s) 1016
ApoI RAATTY 2 cut(s) 512, 637
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
AspS9I GGNCC 3 cut(s) 737, 1012, 1013
AsuHPI GGTGA 2 cut(s) 862, 1049
AvaII GGWCC 1 cut(s) 737
BaeGI GKGCMC 1 cut(s) 1016
BalI TGGCCA 2 cut(s) 117, 301
BanI GGYRCC 1 cut(s) 264
BanII GRGCYC 2 cut(s) 822, 1016
BbsI GAAGAC 1 cut(s) 124
Bbv12I GWGCWC 1 cut(s) 822
BccI CCATC 2 cut(s) 310, 1108
BceAI ACGGC 1 cut(s) 1160
BcoDI GTCTC 3 cut(s) 164, 1016, 1221
BfaI CTAG 2 cut(s) 320, 830
BfmI CTRYAG 1 cut(s) 1017
BfrI CTTAAG 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 761
BisI GCNGC 3 cut(s) 93, 555, 978
BlsI GCNGC 3 cut(s) 94, 556, 979
BmcAI AGTACT 1 cut(s) 809
Bme18I GGWCC 1 cut(s) 737
BmeRI GACNNNNNGTC 2 cut(s) 83, 1148
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 3 cut(s) 737, 1012, 1013
BmiI GGNNCC 4 cut(s) 22, 266, 1013, 1014
BmsI GCATC 4 cut(s) 116, 149, 530, 556
BpiI GAAGAC 1 cut(s) 124
BplI GAGNNNNNCTC 2 cut(s) 1148, 1180
BpmI CTGGAG 1 cut(s) 986
BpuEI CTTGAG 1 cut(s) 543
Bsa29I ATCGAT 1 cut(s) 157
BsaBI GATNNNNATC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 1080
BsaWI WCCGGW 1 cut(s) 397
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 561, 720, 721, 1114
Bse1I ACTGG 2 cut(s) 484, 852
Bse3DI GCAATG 2 cut(s) 978, 1200
Bse8I GATNNNNATC 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 397
BseCI ATCGAT 1 cut(s) 157
BseDI CCNNGG 1 cut(s) 1080
BseGI GGATG 4 cut(s) 46, 342, 730, 946
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 5 cut(s) 56, 561, 720, 721, 1114
BseMI GCAATG 2 cut(s) 978, 1200
BseMII CTCAG 3 cut(s) 782, 835, 1133
BseNI ACTGG 2 cut(s) 484, 852
BseRI GAGGAG 3 cut(s) 182, 448, 830
BseSI GKGCMC 1 cut(s) 1016
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 4 cut(s) 117, 301, 977, 1014
BshNI GGYRCC 1 cut(s) 264
BshVI ATCGAT 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 822
BsiSI CCGG 2 cut(s) 398, 587
BslI CCNNNNNNNGG 5 cut(s) 56, 561, 720, 721, 1114
BsmAI GTCTC 3 cut(s) 164, 1016, 1221
BsnI GGCC 4 cut(s) 117, 301, 977, 1014
Bso31I GGTCTC 1 cut(s) 164
Bsp120I GGGCCC 1 cut(s) 1012
Bsp1286I GDGCHC 2 cut(s) 822, 1016
Bsp13I TCCGGA 1 cut(s) 397
Bsp143I GATC 5 cut(s) 52, 81, 206, 325, 742
BspACI CCGC 6 cut(s) 5, 92, 555, 618, 978, 1109
BspANI GGCC 4 cut(s) 117, 301, 977, 1014
BspCNI CTCAG 3 cut(s) 783, 834, 1132
BspDI ATCGAT 1 cut(s) 157
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 4 cut(s) 22, 266, 1013, 1014
BspMI ACCTGC 1 cut(s) 761
BspPI GGATC 1 cut(s) 214
BspT107I GGYRCC 1 cut(s) 264
BspTI CTTAAG 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 2 cut(s) 557, 980
BsrDI GCAATG 2 cut(s) 978, 1200
BsrI ACTGG 2 cut(s) 484, 852
BssECI CCNNGG 1 cut(s) 1080
BssMI GATC 5 cut(s) 52, 81, 206, 325, 742
BssT1I CCWWGG 1 cut(s) 1080
Bst4CI ACNGT 7 cut(s) 175, 199, 264, 389, 1007, 1027, 1061
BstAFI CTTAAG 1 cut(s) 359
BstC8I GCNNGC 4 cut(s) 287, 411, 573, 1000
BstDEI CTNAG 4 cut(s) 791, 811, 821, 1119
BstF5I GGATG 4 cut(s) 46, 342, 730, 946
BstFNI CGCG 1 cut(s) 92
BstKTI GATC 5 cut(s) 55, 84, 209, 328, 745
BstMAI GTCTC 3 cut(s) 164, 1016, 1221
BstMBI GATC 5 cut(s) 52, 81, 206, 325, 742
BstMWI GCNNNNNNNGC 4 cut(s) 251, 692, 977, 1211
BstNSI RCATGY 3 cut(s) 289, 1002, 1151
BstSFI CTRYAG 1 cut(s) 1017
BstSLI GKGCMC 1 cut(s) 1016
BstUI CGCG 1 cut(s) 92
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
Bsu15I ATCGAT 1 cut(s) 157
BsuRI GGCC 4 cut(s) 117, 301, 977, 1014
BsuTUI ATCGAT 1 cut(s) 157
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 4 cut(s) 46, 342, 730, 946
BtsIMutI CAGTG 1 cut(s) 1012
BveI ACCTGC 1 cut(s) 761
Cac8I GCNNGC 4 cut(s) 287, 411, 573, 1000
Cfr13I GGNCC 3 cut(s) 737, 1012, 1013
ClaI ATCGAT 1 cut(s) 157
CseI GACGC 2 cut(s) 98, 910
Csp6I GTAC 4 cut(s) 711, 808, 899, 959
CviAII CATG 6 cut(s) 113, 242, 286, 295, 999, 1148
CviQI GTAC 4 cut(s) 711, 808, 899, 959
DdeI CTNAG 4 cut(s) 791, 811, 821, 1119
DpnI GATC 5 cut(s) 54, 83, 208, 327, 744
DpnII GATC 5 cut(s) 52, 81, 206, 325, 742
DrdI GACNNNNNNGTC 1 cut(s) 95
DriI GACNNNNNGTC 2 cut(s) 83, 1148
DseDI GACNNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 3 cut(s) 115, 299, 975
Eam1105I GACNNNNNGTC 2 cut(s) 83, 1148
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 820
Eco130I CCWWGG 1 cut(s) 1080
Eco24I GRGCYC 2 cut(s) 822, 1016
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 737
Eco53kI GAGCTC 1 cut(s) 820
Eco57I CTGAAG 2 cut(s) 374, 771
EcoICRI GAGCTC 1 cut(s) 820
EcoO109I RGGNCCY 3 cut(s) 737, 1012, 1013
EcoT14I CCWWGG 1 cut(s) 1080
EcoT38I GRGCYC 2 cut(s) 822, 1016
ErhI CCWWGG 1 cut(s) 1080
FaeI CATG 6 cut(s) 116, 245, 289, 298, 1002, 1151
FatI CATG 6 cut(s) 112, 241, 285, 294, 998, 1147
FauI CCCGC 2 cut(s) 625, 1116
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 3 cut(s) 93, 555, 978
FokI GGATG 4 cut(s) 33, 329, 737, 953
FriOI GRGCYC 2 cut(s) 822, 1016
Fsp4HI GCNGC 3 cut(s) 93, 555, 978
FspBI CTAG 2 cut(s) 320, 830
GluI GCNGC 3 cut(s) 93, 555, 978
GsuI CTGGAG 1 cut(s) 986
HaeIII GGCC 4 cut(s) 117, 301, 977, 1014
HapII CCGG 2 cut(s) 398, 587
HgaI GACGC 2 cut(s) 98, 910
Hin1II CATG 6 cut(s) 116, 245, 289, 298, 1002, 1151
HincII GTYRAC 3 cut(s) 88, 505, 862
HindII GTYRAC 3 cut(s) 88, 505, 862
HindIII AAGCTT 2 cut(s) 356, 753
HinfI GANTC 6 cut(s) 237, 496, 565, 601, 1157, 1192
HpaI GTTAAC 2 cut(s) 505, 862
HpaII CCGG 2 cut(s) 398, 587
HphI GGTGA 2 cut(s) 862, 1049
Hpy166II GTNNAC 4 cut(s) 88, 127, 505, 862
Hpy188I TCNGA 4 cut(s) 792, 824, 1122, 1218
Hpy188III TCNNGA 5 cut(s) 50, 398, 661, 740, 965
Hpy8I GTNNAC 4 cut(s) 88, 127, 505, 862
Hpy99I CGWCG 3 cut(s) 89, 92, 1146
HpyAV CCTTC 4 cut(s) 358, 713, 740, 836
HpyCH4III ACNGT 7 cut(s) 175, 199, 264, 389, 1007, 1027, 1061
HpyCH4IV ACGT 2 cut(s) 33, 129
HpyCH4V TGCA 8 cut(s) 38, 107, 254, 285, 533, 770, 998, 1002
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 692, 977, 1211
HpyF3I CTNAG 4 cut(s) 791, 811, 821, 1119
HpySE526I ACGT 2 cut(s) 33, 129
Hsp92II CATG 6 cut(s) 116, 245, 289, 298, 1002, 1151
Kpn2I TCCGGA 1 cut(s) 397
KspAI GTTAAC 2 cut(s) 505, 862
Kzo9I GATC 5 cut(s) 52, 81, 206, 325, 742
LmnI GCTCC 3 cut(s) 26, 68, 817
LweI GCATC 4 cut(s) 116, 149, 530, 556
MaeI CTAG 2 cut(s) 320, 830
MaeII ACGT 2 cut(s) 33, 129
MaeIII GTNAC 4 cut(s) 193, 1055, 1061, 1150
MalI GATC 5 cut(s) 54, 83, 208, 327, 744
MbiI CCGCTC 2 cut(s) 557, 980
MboI GATC 5 cut(s) 52, 81, 206, 325, 742
MboII GAAGA 3 cut(s) 22, 124, 479
MfeI CAATTG 1 cut(s) 765
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 2 cut(s) 822, 1016
MlsI TGGCCA 2 cut(s) 117, 301
MluCI AATT 4 cut(s) 512, 622, 637, 765
MluNI TGGCCA 2 cut(s) 117, 301
MlyI GAGTC 2 cut(s) 231, 1166
MnlI CCTC 9 cut(s) 11, 160, 426, 429, 657, 808, 883, 961, 972
Mox20I TGGCCA 2 cut(s) 117, 301
MroI TCCGGA 1 cut(s) 397
MscI TGGCCA 2 cut(s) 117, 301
MseI TTAA 6 cut(s) 360, 504, 543, 609, 861, 1029
Msp20I TGGCCA 2 cut(s) 117, 301
MspA1I CMGCKG 1 cut(s) 575
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 2 cut(s) 398, 587
MunI CAATTG 1 cut(s) 765
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 251, 692, 977, 1211
NdeII GATC 5 cut(s) 52, 81, 206, 325, 742
NlaIII CATG 6 cut(s) 116, 245, 289, 298, 1002, 1151
NlaIV GGNNCC 4 cut(s) 22, 266, 1013, 1014
NmuCI GTSAC 2 cut(s) 1055, 1150
NspI RCATGY 3 cut(s) 289, 1002, 1151
PaeI GCATGC 2 cut(s) 289, 1002
PaqCI CACCTGC 1 cut(s) 761
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 4 cut(s) 496, 565, 601, 1192
PkrI GCNGC 3 cut(s) 94, 556, 979
PleI GAGTC 2 cut(s) 231, 1165
PpsI GAGTC 2 cut(s) 231, 1165
PpuMI RGGWCCY 1 cut(s) 737
Psp124BI GAGCTC 1 cut(s) 822
Psp1406I AACGTT 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 737
PspN4I GGNNCC 4 cut(s) 22, 266, 1013, 1014
PspOMI GGGCCC 1 cut(s) 1012
PspPI GGNCC 3 cut(s) 737, 1012, 1013
PspPPI RGGWCCY 1 cut(s) 737
PsuI RGATCY 1 cut(s) 206
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 4 cut(s) 712, 809, 900, 960
RsaNI GTAC 4 cut(s) 711, 808, 899, 959
SacI GAGCTC 1 cut(s) 822
SalI GTCGAC 1 cut(s) 86
SaqAI TTAA 6 cut(s) 360, 504, 543, 609, 861, 1029
SatI GCNGC 3 cut(s) 93, 555, 978
Sau3AI GATC 5 cut(s) 52, 81, 206, 325, 742
Sau96I GGNCC 3 cut(s) 737, 1012, 1013
ScaI AGTACT 1 cut(s) 809
SchI GAGTC 2 cut(s) 231, 1166
SduI GDGCHC 2 cut(s) 822, 1016
SfaNI GCATC 4 cut(s) 116, 149, 530, 556
SfcI CTRYAG 1 cut(s) 1017
SgrDI CGTCGACG 1 cut(s) 86
SinI GGWCC 1 cut(s) 737
SmlI CTYRAG 2 cut(s) 359, 558
SmoI CTYRAG 2 cut(s) 359, 558
SphI GCATGC 2 cut(s) 289, 1002
Sse9I AATT 4 cut(s) 512, 622, 637, 765
SsiI CCGC 6 cut(s) 5, 92, 555, 618, 978, 1109
SspI AATATT 1 cut(s) 1176
SspMI CTAG 2 cut(s) 320, 830
SstI GAGCTC 1 cut(s) 822
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 7 cut(s) 175, 199, 264, 389, 1007, 1027, 1061
TaiI ACGT 2 cut(s) 36, 132
TaqI TCGA 6 cut(s) 87, 99, 157, 235, 660, 780
TasI AATT 4 cut(s) 512, 622, 637, 765
TatI WGTACW 2 cut(s) 807, 898
TauI GCSGC 3 cut(s) 95, 557, 980
TfiI GAWTC 4 cut(s) 496, 565, 601, 1192
Tru1I TTAA 6 cut(s) 360, 504, 543, 609, 861, 1029
Tru9I TTAA 6 cut(s) 360, 504, 543, 609, 861, 1029
TscAI CASTG 1 cut(s) 1012
TseFI GTSAC 2 cut(s) 1055, 1150
Tsp45I GTSAC 2 cut(s) 1055, 1150
TspDTI ATGAA 5 cut(s) 35, 451, 627, 856, 957
TspRI CASTG 1 cut(s) 1012
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 737
XapI RAATTY 2 cut(s) 512, 637
XceI RCATGY 3 cut(s) 289, 1002, 1151
XmiI GTMKAC 1 cut(s) 87
XspI CTAG 2 cut(s) 320, 830
ZrmI AGTACT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.