Rw1G012370

Alpha-1,3 1,6-mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004054
Physical Location & Seq
Forward (+)
27569288 .. 27572095
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G012370.1

Sequence Viewer

Length: 1179 bp
ATGAAACTTACAGCCTGCGCCGACGAAATAGAGCAGACGAAGCAGCTTCGGCGAGGAGCTGAAAGACTAATTGTGGATGCGGCTGTTGAGCTCGCATCGCATGGCCATAAAGTTCACGTCTTTACCTCACACCATGATAAAAATCGATGCTTTGAGGAGACCATCTCTGGTGCCTTTCCTGTTACTGTGTACGGTGCTTTTCTACCCCGACATGTATTCTATCGTCTTCATGCTTTGTGTGCATACCTACGGTGCCTGTATGTTGCCCTCTGCGTGCTAGTCATGTGGCCGTCTTTTGATGTAATACTAGTAGATCAGGTTTCTGTTGTCATCCCATTGCTGAAGCTTAAGAAGTCGGCAAAGGTGTTGTTTTATTGTCATTTTCCAGATTTGTTGCTCGCTCAACACACAACTATTCTGAGGAGGATATATAGAAAACCCATAGACTTCATAGAAGAAATGACAACTGGAATGGCAGATAAGATTCTTGTTAACAGCAAATTTACAGCACTTACATTTGCAAGGACATTCAAGCATCTTGATGCACAAGGAATTAAGCCAGCTGTTCTTCACCCAGCTGTCAATGTGAATCAATTCGATGAACCTAGCAATTCTCCTAAGTTAAATTTTCTCTCCATCAACCGTTTTGAGAGGAAAAAGAATATAGACTTGGCAATTTCAGCTTTTGCCATGCTTCGCACCCTTGAAGGAGATATGCTTCAAGGTCTTAATCTGGCTGAAGCTTCCTTGACAATTGCAGGTGGCTTTGACAATCGGTTGAGAGAAAATGTTGAGTACTTGGAGGAGCTCAGAAGTTTAGCAGAAAGGGAAGGAGTGTCCAATCAGGTTAACTTCATCACATCTTGCTCAACAGCCGAAAGAAATGCACTACTCTCCCAGTGCCTATGTGTCGTTTATACACCAAAGGATGAACACTTTGGCATTGTTCCTCTGGAGGCAATGGCAGCTCATAAACCAGTTATTGCGTGCAACAGTGGGGGTCCTGTACAGACAGTTAAGAATGGCGAAACGGGATTTCTTTGTGAAAGTAACCCAAGAGAGTTTTCCTTGGCTATGGCTAAACTCATTCAAGACCCACAGATGGCACAACGAATGGGTGTAGAAGCTCGGTGGCATGTTACAGAGTCATTCTCTACAAAAAATTCGGTCAGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko00513 ko01100 map00510 map00513 map01100
Pfam Domains
Protein Families
Glyco_transf_4 Glycos_transf_1

Protein Analysis

392

Amino Acids

43.94

Weight (kDa)

7.98

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_4 PF13439 19 - 195 3.2e-12 Glycosyltransferase Family 4
Glyco_trans_4_4 PF13579 19 - 175 6.8e-06 Glycosyl transferase 4-like domain
GT4-conflict PF20706 183 - 339 7.1e-09 Family 4 Glycosyltransferase in conflict systems
Glycos_transf_1 PF00534 198 - 377 1.2e-35 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 210 - 365 7.2e-20 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 749
Acc36I ACCTGC 1 cut(s) 749
AccB1I GGYRCC 2 cut(s) 170, 252
AciI CCGC 1 cut(s) 80
AcoI YGGCCR 2 cut(s) 103, 287
AcsI RAATTY 3 cut(s) 500, 625, 1162
AcuI CTGAAG 2 cut(s) 362, 759
AfaI GTAC 3 cut(s) 191, 797, 1008
AfiI CCNNNNNNNGG 1 cut(s) 1102
AflII CTTAAG 1 cut(s) 347
AflIII ACRYGT 1 cut(s) 211
AgsI TTSAA 4 cut(s) 532, 707, 722, 1091
AhlI ACTAGT 1 cut(s) 307
AjiI CACGTC 1 cut(s) 118
AjuI GAANNNNNNNTTGG 4 cut(s) 653, 685, 1048, 1080
Alw21I GWGCWC 2 cut(s) 93, 810
Alw26I GTCTC 1 cut(s) 152
AoxI GGCC 2 cut(s) 103, 287
ApeKI GCWGC 2 cut(s) 43, 965
ApoI RAATTY 3 cut(s) 500, 625, 1162
Asp700I GAANNNNTTC 1 cut(s) 593
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 1001
AsuHPI GGTGA 1 cut(s) 563
AvaII GGWCC 1 cut(s) 1001
BalI TGGCCA 1 cut(s) 105
BanI GGYRCC 2 cut(s) 170, 252
BanII GRGCYC 2 cut(s) 93, 810
BbsI GAAGAC 1 cut(s) 218
Bbv12I GWGCWC 2 cut(s) 93, 810
BbvI GCAGC 2 cut(s) 55, 977
BccI CCATC 3 cut(s) 170, 644, 1096
BceAI ACGGC 1 cut(s) 274
BcgI CGANNNNNNTGC 2 cut(s) 866, 900
BcoDI GTCTC 1 cut(s) 152
BcuI ACTAGT 1 cut(s) 307
BfaI CTAG 3 cut(s) 278, 308, 606
BfrI CTTAAG 1 cut(s) 347
BfuAI ACCTGC 1 cut(s) 749
BisI GCNGC 3 cut(s) 44, 81, 966
BlsI GCNGC 3 cut(s) 45, 82, 967
BmcAI AGTACT 1 cut(s) 797
Bme18I GGWCC 1 cut(s) 1001
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 1001
BmiI GGNNCC 3 cut(s) 172, 254, 1002
BmrI ACTGGG 1 cut(s) 892
BmsI GCATC 5 cut(s) 67, 104, 137, 532, 544
BmuI ACTGGG 1 cut(s) 892
BpiI GAAGAC 1 cut(s) 218
BplI GAGNNNNNCTC 4 cut(s) 149, 181, 1136, 1168
BpmI CTGGAG 1 cut(s) 974
Bsa29I ATCGAT 1 cut(s) 145
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 1068
Bsc4I CCNNNNNNNGG 1 cut(s) 1102
Bse1I ACTGG 3 cut(s) 472, 898, 977
Bse3DI GCAATG 2 cut(s) 335, 966
Bse8I GATNNNNATC 1 cut(s) 141
BseCI ATCGAT 1 cut(s) 145
BseDI CCNNGG 1 cut(s) 1068
BseGI GGATG 3 cut(s) 82, 330, 934
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 1102
BseMI GCAATG 2 cut(s) 335, 966
BseMII CTCAG 2 cut(s) 410, 823
BseNI ACTGG 3 cut(s) 472, 898, 977
BseRI GAGGAG 4 cut(s) 69, 170, 436, 818
BseXI GCAGC 2 cut(s) 55, 977
BseYI CCCAGC 1 cut(s) 574
BshFI GGCC 2 cut(s) 105, 289
BshNI GGYRCC 2 cut(s) 170, 252
BshVI ATCGAT 1 cut(s) 145
BsiHKAI GWGCWC 2 cut(s) 93, 810
BslI CCNNNNNNNGG 1 cut(s) 1102
BsmAI GTCTC 1 cut(s) 152
BsnI GGCC 2 cut(s) 105, 289
Bso31I GGTCTC 1 cut(s) 152
Bsp1286I GDGCHC 2 cut(s) 93, 810
Bsp1407I TGTACA 1 cut(s) 1006
Bsp143I GATC 1 cut(s) 313
BspACI CCGC 1 cut(s) 80
BspANI GGCC 2 cut(s) 105, 289
BspCNI CTCAG 2 cut(s) 411, 822
BspDI ATCGAT 1 cut(s) 145
BspLI GGNNCC 3 cut(s) 172, 254, 1002
BspMI ACCTGC 1 cut(s) 749
BspT107I GGYRCC 2 cut(s) 170, 252
BspTI CTTAAG 1 cut(s) 347
BspTNI GGTCTC 1 cut(s) 152
BsrDI GCAATG 2 cut(s) 335, 966
BsrGI TGTACA 1 cut(s) 1006
BsrI ACTGG 3 cut(s) 472, 898, 977
BssECI CCNNGG 1 cut(s) 1068
BssMI GATC 1 cut(s) 313
BssT1I CCWWGG 1 cut(s) 1068
Bst4CI ACNGT 6 cut(s) 187, 194, 252, 644, 995, 1015
BstAFI CTTAAG 1 cut(s) 347
BstAUI TGTACA 1 cut(s) 1006
BstC8I GCNNGC 6 cut(s) 16, 93, 275, 399, 561, 988
BstDEI CTNAG 3 cut(s) 419, 618, 809
BstF5I GGATG 3 cut(s) 82, 330, 934
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 1 cut(s) 316
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 1 cut(s) 313
BstMWI GCNNNNNNNGC 6 cut(s) 40, 49, 97, 239, 680, 965
BstNSI RCATGY 2 cut(s) 215, 1139
BstV1I GCAGC 2 cut(s) 55, 977
BstV2I GAAGAC 1 cut(s) 218
Bsu15I ATCGAT 1 cut(s) 145
BsuRI GGCC 2 cut(s) 105, 289
BsuTUI ATCGAT 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 81
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 3 cut(s) 82, 330, 934
BtsIMutI CAGTG 2 cut(s) 905, 1000
BveI ACCTGC 1 cut(s) 749
Cac8I GCNNGC 6 cut(s) 16, 93, 275, 399, 561, 988
CfoI GCGC 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 1001
ClaI ATCGAT 1 cut(s) 145
Csp6I GTAC 3 cut(s) 190, 796, 1007
CviAII CATG 7 cut(s) 101, 134, 212, 230, 283, 691, 1136
CviQI GTAC 3 cut(s) 190, 796, 1007
DdeI CTNAG 3 cut(s) 419, 618, 809
DpnI GATC 1 cut(s) 315
DpnII GATC 1 cut(s) 313
EaeI YGGCCR 2 cut(s) 103, 287
Ecl136II GAGCTC 2 cut(s) 91, 808
Eco130I CCWWGG 1 cut(s) 1068
Eco24I GRGCYC 2 cut(s) 93, 810
Eco31I GGTCTC 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 1001
Eco53kI GAGCTC 2 cut(s) 91, 808
Eco57I CTGAAG 2 cut(s) 362, 759
EcoICRI GAGCTC 2 cut(s) 91, 808
EcoO109I RGGNCCY 1 cut(s) 1001
EcoT14I CCWWGG 1 cut(s) 1068
EcoT38I GRGCYC 2 cut(s) 93, 810
ErhI CCWWGG 1 cut(s) 1068
FaeI CATG 7 cut(s) 104, 137, 215, 233, 286, 694, 1139
FatI CATG 7 cut(s) 100, 133, 211, 229, 282, 690, 1135
Fnu4HI GCNGC 3 cut(s) 44, 81, 966
FokI GGATG 3 cut(s) 89, 317, 941
FriOI GRGCYC 2 cut(s) 93, 810
Fsp4HI GCNGC 3 cut(s) 44, 81, 966
FspBI CTAG 3 cut(s) 278, 308, 606
GlaI GCGC 1 cut(s) 19
GluI GCNGC 3 cut(s) 44, 81, 966
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 1 cut(s) 974
HaeIII GGCC 2 cut(s) 105, 289
HhaI GCGC 1 cut(s) 20
Hin1II CATG 7 cut(s) 104, 137, 215, 233, 286, 694, 1139
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HincII GTYRAC 2 cut(s) 493, 850
HindII GTYRAC 2 cut(s) 493, 850
HindIII AAGCTT 2 cut(s) 344, 741
HinfI GANTC 3 cut(s) 484, 589, 1145
HpaI GTTAAC 2 cut(s) 493, 850
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 4 cut(s) 115, 190, 493, 850
Hpy188I TCNGA 2 cut(s) 420, 812
Hpy188III TCNNGA 4 cut(s) 386, 539, 953, 1091
Hpy8I GTNNAC 4 cut(s) 115, 190, 493, 850
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 2 cut(s) 701, 824
HpyCH4III ACNGT 6 cut(s) 187, 194, 252, 644, 995, 1015
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 6 cut(s) 242, 521, 545, 758, 887, 990
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 49, 97, 239, 680, 965
HpyF3I CTNAG 3 cut(s) 419, 618, 809
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 7 cut(s) 104, 137, 215, 233, 286, 694, 1139
HspAI GCGC 1 cut(s) 18
KspAI GTTAAC 2 cut(s) 493, 850
Kzo9I GATC 1 cut(s) 313
LmnI GCTCC 2 cut(s) 56, 805
Lsp1109I GCAGC 2 cut(s) 55, 977
LweI GCATC 5 cut(s) 67, 104, 137, 532, 544
MaeI CTAG 3 cut(s) 278, 308, 606
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 3 cut(s) 181, 1049, 1138
MalI GATC 1 cut(s) 315
MboI GATC 1 cut(s) 313
MboII GAAGA 3 cut(s) 218, 467, 560
MfeI CAATTG 1 cut(s) 753
MhlI GDGCHC 2 cut(s) 93, 810
MlsI TGGCCA 1 cut(s) 105
MluCI AATT 9 cut(s) 69, 500, 552, 593, 610, 625, 675, 753, 1162
MluNI TGGCCA 1 cut(s) 105
MlyI GAGTC 1 cut(s) 1154
Mox20I TGGCCA 1 cut(s) 105
MroXI GAANNNNTTC 1 cut(s) 593
MscI TGGCCA 1 cut(s) 105
MseI TTAA 7 cut(s) 348, 492, 555, 623, 729, 849, 1017
MslI CAYNNNNRTG 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 105
MspA1I CMGCKG 2 cut(s) 563, 578
MspCI CTTAAG 1 cut(s) 347
MunI CAATTG 1 cut(s) 753
MwoI GCNNNNNNNGC 6 cut(s) 40, 49, 97, 239, 680, 965
NdeII GATC 1 cut(s) 313
NlaIII CATG 7 cut(s) 104, 137, 215, 233, 286, 694, 1139
NlaIV GGNNCC 3 cut(s) 172, 254, 1002
NspI RCATGY 2 cut(s) 215, 1139
PaqCI CACCTGC 1 cut(s) 749
PciI ACATGT 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 484, 589
PkrI GCNGC 3 cut(s) 45, 82, 967
PleI GAGTC 1 cut(s) 1153
PpsI GAGTC 1 cut(s) 1153
PpuMI RGGWCCY 1 cut(s) 1001
PscI ACATGT 1 cut(s) 211
Psp124BI GAGCTC 2 cut(s) 93, 810
Psp5II RGGWCCY 1 cut(s) 1001
PspFI CCCAGC 1 cut(s) 574
PspN4I GGNNCC 3 cut(s) 172, 254, 1002
PspPI GGNCC 1 cut(s) 1001
PspPPI RGGWCCY 1 cut(s) 1001
PvuII CAGCTG 2 cut(s) 563, 578
RsaI GTAC 3 cut(s) 191, 797, 1008
RsaNI GTAC 3 cut(s) 190, 796, 1007
RseI CAYNNNNRTG 1 cut(s) 540
SacI GAGCTC 2 cut(s) 93, 810
SaqAI TTAA 7 cut(s) 348, 492, 555, 623, 729, 849, 1017
SatI GCNGC 3 cut(s) 44, 81, 966
Sau3AI GATC 1 cut(s) 313
Sau96I GGNCC 1 cut(s) 1001
ScaI AGTACT 1 cut(s) 797
SchI GAGTC 1 cut(s) 1154
SduI GDGCHC 2 cut(s) 93, 810
SfaNI GCATC 5 cut(s) 67, 104, 137, 532, 544
SinI GGWCC 1 cut(s) 1001
SmiMI CAYNNNNRTG 1 cut(s) 540
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SpeI ACTAGT 1 cut(s) 307
Sse9I AATT 9 cut(s) 69, 500, 552, 593, 610, 625, 675, 753, 1162
SsiI CCGC 1 cut(s) 80
SspMI CTAG 3 cut(s) 278, 308, 606
SstI GAGCTC 2 cut(s) 93, 810
StyI CCWWGG 1 cut(s) 1068
TaaI ACNGT 6 cut(s) 187, 194, 252, 644, 995, 1015
TaiI ACGT 1 cut(s) 120
TaqI TCGA 2 cut(s) 145, 597
TaqII GACCGA 1 cut(s) 1156
TasI AATT 9 cut(s) 69, 500, 552, 593, 610, 625, 675, 753, 1162
TatI WGTACW 2 cut(s) 795, 1006
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 2 cut(s) 484, 589
Tru1I TTAA 7 cut(s) 348, 492, 555, 623, 729, 849, 1017
Tru9I TTAA 7 cut(s) 348, 492, 555, 623, 729, 849, 1017
TscAI CASTG 2 cut(s) 905, 1000
TseI GCWGC 2 cut(s) 43, 965
TspDTI ATGAA 6 cut(s) 17, 218, 439, 615, 844, 945
TspRI CASTG 2 cut(s) 905, 1000
Vha464I CTTAAG 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 1001
XapI RAATTY 3 cut(s) 500, 625, 1162
XceI RCATGY 2 cut(s) 215, 1139
XmnI GAANNNNTTC 1 cut(s) 593
XspI CTAG 3 cut(s) 278, 308, 606
ZrmI AGTACT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.