Rh5CG235200

Alpha-1,3 1,6-mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0004054
Physical Location & Seq
Reverse (-)
24519217 .. 24524025
4809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG235200.1

Sequence Viewer

Length: 1242 bp
ATGTCTAAAATGGCGAAGAGAGGAGGAAGCTCAACGTTGAACGTCGCTGTTATTCATCCCGATCTGGGTATAGGAGGAGCTGAAAGACTAATTGTGGATGCGGCTGTTGAACTCGCATCGCATGGCCATAAAGTTCACGTCTTTACCTCACACCATGATAAAAATCGATGCTTTGAGGAGACCATCTCTGGTGCCTTTCCTGTTACTGTGTACGGTGCTTTCCTACCCCGACATGTATTCTATCGTCTTCATGCTTTGTGTGCATACCTACGGTGCCTGTATGTTGCCCTCTGCGTGCTAGTCATGTGGCCGTCTTTTGATGTAATACTAGTAGATCAGGTTTCTGTTGTCATCCCATTGCTGAAGCTTAAGAAGTCGGCAAAGGTGTTGTTTTATTGTCATTTTCCAGATTTATTGCTCGCTCAACACACAACTATTCTGAGGAGGATATATAGAAAACCCATAGACTTCATAGAAGAAATGACAACTGGAATGGCAGATAAGATTCTTGTTAACAGCAAATTTACAGCACTTACATTTGCAAAGACATTCAAGCATCTTGATGCACGAGGAATTAAGCCAGCTGTTCTTCACCCAGCTGTCAATGTGAATCAATTCGATGAACCTAGCAATTCTCCTAAGTTAAATTTTCTCTCCATCAACCGTTTTGAGAGGAAAAAGAATGTAGACTTGGCAATTTCATCTTTTGCCATGCTTCGCACCCTTGAAGGAGATATGCTTCAAGGTCTTAATCTGGCTGAAGCTTCCTTGACAATTGCAGGTGGCTTTGACAACCGGTTGAGAGAAAATGTTGAGTACTTGGAGGAGCTCAGAAGTTTAGCAGAAAGGGAAGGAGTGTCCAATCAGGTTAACTTCATCACATCTTGCTCAACAGCCGAAAGAAATGCACTACTCTCCCAGTGCCTGTGTGTCATTTATACACCAAAGGATGAACACTTTGGCATTGTTCCTCTGGAGGCAATGGCAGCTCATAAACCAGTTATTGCGTGCAACAGTGGGGGTCCTGTAGAGACAGTTAAGAATGGCGAAACGGGATTTCTTTGTGAAAGTAACCCAAGAGAGTTTTCCTTGGCTATGGCTAAACTCATTCAAGACCCACAGATGGCGCAACGAATGGGTGTAGAAGCTAGGCGGCATGTTACAGAGTCATTCTCTACAAAAATATTCGGTCAGCATTTGAATCAATACCTAGTTGATGTTGCTCGGGCTAAAGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko00513 ko01100 map00510 map00513 map01100
Pfam Domains
Protein Families
Glyco_transf_4 Glycos_transf_1

Protein Analysis

413

Amino Acids

46.03

Weight (kDa)

8.27

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_4 PF13439 24 - 202 2.7e-13 Glycosyltransferase Family 4
Glyco_trans_4_4 PF13579 25 - 178 3.8e-06 Glycosyl transferase 4-like domain
GT4-conflict PF20706 190 - 348 4.1e-08 Family 4 Glycosyltransferase in conflict systems
Glycos_transf_1 PF00534 205 - 385 1.3e-35 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 217 - 372 1.3e-19 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 770
Acc36I ACCTGC 1 cut(s) 770
AccB1I GGYRCC 2 cut(s) 191, 273
AccI GTMKAC 1 cut(s) 687
AciI CCGC 2 cut(s) 101, 1153
AclI AACGTT 1 cut(s) 35
AcoI YGGCCR 2 cut(s) 124, 308
AcsI RAATTY 2 cut(s) 521, 646
AcuI CTGAAG 2 cut(s) 383, 780
AfaI GTAC 2 cut(s) 212, 818
AfiI CCNNNNNNNGG 2 cut(s) 65, 1123
AflII CTTAAG 1 cut(s) 368
AflIII ACRYGT 1 cut(s) 232
AgeI ACCGGT 1 cut(s) 795
AgsI TTSAA 7 cut(s) 40, 110, 553, 728, 743, 1112, 1201
AhlI ACTAGT 1 cut(s) 328
AjiI CACGTC 1 cut(s) 139
AjuI GAANNNNNNNTTGG 4 cut(s) 674, 706, 1069, 1101
AluBI AGCT 9 cut(s) 30, 80, 367, 584, 599, 764, 829, 989, 1148
AluI AGCT 9 cut(s) 30, 80, 367, 584, 599, 764, 829, 989, 1148
Alw21I GWGCWC 1 cut(s) 831
Alw26I GTCTC 2 cut(s) 173, 1025
AlwNI CAGNNNCTG 1 cut(s) 925
Ama87I CYCGRG 1 cut(s) 1224
AoxI GGCC 2 cut(s) 124, 308
ApeKI GCWGC 1 cut(s) 986
ApoI RAATTY 2 cut(s) 521, 646
AsiGI ACCGGT 1 cut(s) 795
Asp700I GAANNNNTTC 1 cut(s) 614
AspLEI GCGC 1 cut(s) 1129
AspS9I GGNCC 1 cut(s) 1022
AsuHPI GGTGA 1 cut(s) 584
AvaI CYCGRG 1 cut(s) 1224
AvaII GGWCC 1 cut(s) 1022
BalI TGGCCA 1 cut(s) 126
BanI GGYRCC 2 cut(s) 191, 273
BanII GRGCYC 1 cut(s) 831
BauI CACGAG 1 cut(s) 567
BbsI GAAGAC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 831
BbvI GCAGC 1 cut(s) 998
BccI CCATC 3 cut(s) 191, 665, 1117
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 887, 921
BcoDI GTCTC 2 cut(s) 173, 1025
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 5 cut(s) 299, 329, 627, 1149, 1211
BfmI CTRYAG 1 cut(s) 1026
BfrI CTTAAG 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 770
BisI GCNGC 3 cut(s) 102, 987, 1154
BlsI GCNGC 3 cut(s) 103, 988, 1155
BmcAI AGTACT 1 cut(s) 818
Bme18I GGWCC 1 cut(s) 1022
BmeT110I CYCGRG 1 cut(s) 1224
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 1022
BmiI GGNNCC 3 cut(s) 193, 275, 1023
BmrI ACTGGG 1 cut(s) 913
BmsI GCATC 5 cut(s) 88, 125, 158, 553, 565
BmuI ACTGGG 1 cut(s) 913
BpiI GAAGAC 1 cut(s) 239
BplI GAGNNNNNCTC 4 cut(s) 170, 202, 1157, 1189
BpmI CTGGAG 1 cut(s) 995
Bsa29I ATCGAT 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 1089
BsaWI WCCGGW 1 cut(s) 795
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 1123
Bse118I RCCGGY 1 cut(s) 795
Bse1I ACTGG 3 cut(s) 493, 919, 998
Bse3DI GCAATG 2 cut(s) 356, 987
Bse8I GATNNNNATC 1 cut(s) 162
BseCI ATCGAT 1 cut(s) 166
BseDI CCNNGG 1 cut(s) 1089
BseGI GGATG 4 cut(s) 55, 103, 351, 955
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 2 cut(s) 65, 1123
BseMI GCAATG 2 cut(s) 356, 987
BseMII CTCAG 2 cut(s) 431, 844
BseNI ACTGG 3 cut(s) 493, 919, 998
BseRI GAGGAG 5 cut(s) 36, 90, 191, 457, 839
BseXI GCAGC 1 cut(s) 998
BseYI CCCAGC 1 cut(s) 595
BshFI GGCC 2 cut(s) 126, 310
BshNI GGYRCC 2 cut(s) 191, 273
BshTI ACCGGT 1 cut(s) 795
BshVI ATCGAT 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 831
BsiHKCI CYCGRG 1 cut(s) 1224
BsiSI CCGG 1 cut(s) 796
BslI CCNNNNNNNGG 2 cut(s) 65, 1123
BsmAI GTCTC 2 cut(s) 173, 1025
BsnI GGCC 2 cut(s) 126, 310
Bso31I GGTCTC 1 cut(s) 173
BsoBI CYCGRG 1 cut(s) 1224
Bsp1286I GDGCHC 1 cut(s) 831
Bsp143I GATC 2 cut(s) 61, 334
BspACI CCGC 2 cut(s) 101, 1153
BspANI GGCC 2 cut(s) 126, 310
BspCNI CTCAG 2 cut(s) 432, 843
BspDI ATCGAT 1 cut(s) 166
BspLI GGNNCC 3 cut(s) 193, 275, 1023
BspMI ACCTGC 1 cut(s) 770
BspT107I GGYRCC 2 cut(s) 191, 273
BspTI CTTAAG 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 356, 987
BsrFI RCCGGY 1 cut(s) 795
BsrI ACTGG 3 cut(s) 493, 919, 998
BssAI RCCGGY 1 cut(s) 795
BssECI CCNNGG 1 cut(s) 1089
BssMI GATC 2 cut(s) 61, 334
BssSI CACGAG 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 1089
Bst2BI CACGAG 1 cut(s) 567
Bst4CI ACNGT 6 cut(s) 208, 215, 273, 665, 1016, 1036
Bst6I CTCTTC 1 cut(s) 11
BstAFI CTTAAG 1 cut(s) 368
BstC8I GCNNGC 4 cut(s) 296, 420, 582, 1009
BstDEI CTNAG 3 cut(s) 440, 639, 830
BstF5I GGATG 4 cut(s) 55, 103, 351, 955
BstHHI GCGC 1 cut(s) 1129
BstKTI GATC 2 cut(s) 64, 337
BstMAI GTCTC 2 cut(s) 173, 1025
BstMBI GATC 2 cut(s) 61, 334
BstMWI GCNNNNNNNGC 2 cut(s) 260, 986
BstNSI RCATGY 2 cut(s) 236, 1160
BstSFI CTRYAG 1 cut(s) 1026
BstV1I GCAGC 1 cut(s) 998
BstV2I GAAGAC 1 cut(s) 239
Bsu15I ATCGAT 1 cut(s) 166
BsuRI GGCC 2 cut(s) 126, 310
BsuTUI ATCGAT 1 cut(s) 166
BtgZI GCGATG 1 cut(s) 102
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 4 cut(s) 55, 103, 351, 955
BtsIMutI CAGTG 2 cut(s) 926, 1021
BveI ACCTGC 1 cut(s) 770
Cac8I GCNNGC 4 cut(s) 296, 420, 582, 1009
CaiI CAGNNNCTG 1 cut(s) 925
CfoI GCGC 1 cut(s) 1129
Cfr10I RCCGGY 1 cut(s) 795
Cfr13I GGNCC 1 cut(s) 1022
ClaI ATCGAT 1 cut(s) 166
Csp6I GTAC 2 cut(s) 211, 817
CspAI ACCGGT 1 cut(s) 795
CviAII CATG 7 cut(s) 122, 155, 233, 251, 304, 712, 1157
CviQI GTAC 2 cut(s) 211, 817
DdeI CTNAG 3 cut(s) 440, 639, 830
DpnI GATC 2 cut(s) 63, 336
DpnII GATC 2 cut(s) 61, 334
EaeI YGGCCR 2 cut(s) 124, 308
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Ecl136II GAGCTC 1 cut(s) 829
Eco130I CCWWGG 1 cut(s) 1089
Eco24I GRGCYC 1 cut(s) 831
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 1022
Eco53kI GAGCTC 1 cut(s) 829
Eco57I CTGAAG 2 cut(s) 383, 780
Eco88I CYCGRG 1 cut(s) 1224
EcoICRI GAGCTC 1 cut(s) 829
EcoO109I RGGNCCY 1 cut(s) 1022
EcoT14I CCWWGG 1 cut(s) 1089
EcoT38I GRGCYC 1 cut(s) 831
ErhI CCWWGG 1 cut(s) 1089
FaeI CATG 7 cut(s) 125, 158, 236, 254, 307, 715, 1160
FatI CATG 7 cut(s) 121, 154, 232, 250, 303, 711, 1156
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 3 cut(s) 102, 987, 1154
FokI GGATG 4 cut(s) 42, 110, 338, 962
FriOI GRGCYC 1 cut(s) 831
Fsp4HI GCNGC 3 cut(s) 102, 987, 1154
FspBI CTAG 5 cut(s) 299, 329, 627, 1149, 1211
GlaI GCGC 1 cut(s) 1128
GluI GCNGC 3 cut(s) 102, 987, 1154
GsaI CCCAGC 1 cut(s) 599
GsuI CTGGAG 1 cut(s) 995
HaeIII GGCC 2 cut(s) 126, 310
HapII CCGG 1 cut(s) 796
HhaI GCGC 1 cut(s) 1129
Hin1II CATG 7 cut(s) 125, 158, 236, 254, 307, 715, 1160
Hin6I GCGC 1 cut(s) 1127
HinP1I GCGC 1 cut(s) 1127
HincII GTYRAC 2 cut(s) 514, 871
HindII GTYRAC 2 cut(s) 514, 871
HindIII AAGCTT 2 cut(s) 365, 762
HinfI GANTC 4 cut(s) 505, 610, 1166, 1201
HpaI GTTAAC 2 cut(s) 514, 871
HpaII CCGG 1 cut(s) 796
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 5 cut(s) 136, 211, 514, 688, 871
Hpy188I TCNGA 2 cut(s) 441, 833
Hpy188III TCNNGA 5 cut(s) 59, 407, 560, 974, 1112
Hpy8I GTNNAC 5 cut(s) 136, 211, 514, 688, 871
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 2 cut(s) 722, 845
HpyCH4III ACNGT 6 cut(s) 208, 215, 273, 665, 1016, 1036
HpyCH4IV ACGT 3 cut(s) 35, 42, 138
HpyCH4V TGCA 6 cut(s) 263, 542, 566, 779, 908, 1011
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 986
HpyF3I CTNAG 3 cut(s) 440, 639, 830
HpySE526I ACGT 3 cut(s) 35, 42, 138
Hsp92II CATG 7 cut(s) 125, 158, 236, 254, 307, 715, 1160
HspAI GCGC 1 cut(s) 1127
KspAI GTTAAC 2 cut(s) 514, 871
Kzo9I GATC 2 cut(s) 61, 334
LmnI GCTCC 2 cut(s) 77, 826
Lsp1109I GCAGC 1 cut(s) 998
LweI GCATC 5 cut(s) 88, 125, 158, 553, 565
MaeI CTAG 5 cut(s) 299, 329, 627, 1149, 1211
MaeII ACGT 3 cut(s) 35, 42, 138
MaeIII GTNAC 3 cut(s) 202, 1070, 1159
MalI GATC 2 cut(s) 63, 336
MboI GATC 2 cut(s) 61, 334
MboII GAAGA 4 cut(s) 28, 239, 488, 581
MfeI CAATTG 1 cut(s) 774
MhlI GDGCHC 1 cut(s) 831
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 8 cut(s) 90, 521, 573, 614, 631, 646, 696, 774
MluNI TGGCCA 1 cut(s) 126
MlyI GAGTC 1 cut(s) 1175
Mox20I TGGCCA 1 cut(s) 126
MroXI GAANNNNTTC 1 cut(s) 614
MscI TGGCCA 1 cut(s) 126
MseI TTAA 7 cut(s) 369, 513, 576, 644, 750, 870, 1038
MslI CAYNNNNRTG 1 cut(s) 561
Msp20I TGGCCA 1 cut(s) 126
MspA1I CMGCKG 2 cut(s) 584, 599
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 1 cut(s) 796
MunI CAATTG 1 cut(s) 774
MwoI GCNNNNNNNGC 2 cut(s) 260, 986
NdeII GATC 2 cut(s) 61, 334
NlaIII CATG 7 cut(s) 125, 158, 236, 254, 307, 715, 1160
NlaIV GGNNCC 3 cut(s) 193, 275, 1023
NspI RCATGY 2 cut(s) 236, 1160
PaqCI CACCTGC 1 cut(s) 770
PciI ACATGT 1 cut(s) 232
PdmI GAANNNNTTC 1 cut(s) 614
PfeI GAWTC 3 cut(s) 505, 610, 1201
PinAI ACCGGT 1 cut(s) 795
PkrI GCNGC 3 cut(s) 103, 988, 1155
PleI GAGTC 1 cut(s) 1174
PpsI GAGTC 1 cut(s) 1174
PpuMI RGGWCCY 1 cut(s) 1022
PscI ACATGT 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 831
Psp1406I AACGTT 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 1022
PspFI CCCAGC 1 cut(s) 595
PspN4I GGNNCC 3 cut(s) 193, 275, 1023
PspPI GGNCC 1 cut(s) 1022
PspPPI RGGWCCY 1 cut(s) 1022
PstNI CAGNNNCTG 1 cut(s) 925
PvuII CAGCTG 2 cut(s) 584, 599
RsaI GTAC 2 cut(s) 212, 818
RsaNI GTAC 2 cut(s) 211, 817
RseI CAYNNNNRTG 1 cut(s) 561
SacI GAGCTC 1 cut(s) 831
SaqAI TTAA 7 cut(s) 369, 513, 576, 644, 750, 870, 1038
SatI GCNGC 3 cut(s) 102, 987, 1154
Sau3AI GATC 2 cut(s) 61, 334
Sau96I GGNCC 1 cut(s) 1022
ScaI AGTACT 1 cut(s) 818
SchI GAGTC 1 cut(s) 1175
SduI GDGCHC 1 cut(s) 831
SfaNI GCATC 5 cut(s) 88, 125, 158, 553, 565
SfcI CTRYAG 1 cut(s) 1026
SinI GGWCC 1 cut(s) 1022
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
SpeI ACTAGT 1 cut(s) 328
Sse9I AATT 8 cut(s) 90, 521, 573, 614, 631, 646, 696, 774
SsiI CCGC 2 cut(s) 101, 1153
SspI AATATT 1 cut(s) 1185
SspMI CTAG 5 cut(s) 299, 329, 627, 1149, 1211
SstI GAGCTC 1 cut(s) 831
StyI CCWWGG 1 cut(s) 1089
TaaI ACNGT 6 cut(s) 208, 215, 273, 665, 1016, 1036
TaiI ACGT 3 cut(s) 38, 45, 141
TaqI TCGA 2 cut(s) 166, 618
TaqII GACCGA 1 cut(s) 1178
TasI AATT 8 cut(s) 90, 521, 573, 614, 631, 646, 696, 774
TatI WGTACW 1 cut(s) 816
TauI GCSGC 2 cut(s) 104, 1156
TfiI GAWTC 3 cut(s) 505, 610, 1201
Tru1I TTAA 7 cut(s) 369, 513, 576, 644, 750, 870, 1038
Tru9I TTAA 7 cut(s) 369, 513, 576, 644, 750, 870, 1038
TscAI CASTG 2 cut(s) 926, 1021
TseI GCWGC 1 cut(s) 986
TspDTI ATGAA 7 cut(s) 44, 239, 460, 636, 690, 865, 966
TspRI CASTG 2 cut(s) 926, 1021
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 1022
XapI RAATTY 2 cut(s) 521, 646
XceI RCATGY 2 cut(s) 236, 1160
XmiI GTMKAC 1 cut(s) 687
XmnI GAANNNNTTC 1 cut(s) 614
XspI CTAG 5 cut(s) 299, 329, 627, 1149, 1211
ZrmI AGTACT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.