Rh5AG212700

Alpha-1,3 1,6-mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004054
Physical Location & Seq
Reverse (-)
25126954 .. 25132444
5491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG212700.1

Sequence Viewer

Length: 1233 bp
ATGGCGAAGAGAGGAGGAAGCTCAACGTTGAACGTCGCTGTTATTCATCCCGATCTGGGTATAGGAGGAGCTGAAAGACTAATTGTGGATGCGGCTGTTGAGCTCGCATCGCATGGCCATAAAGTTCACGTCTTTACCTCACACCATGATAAAAATCGATGCTTTGAGGAGACCGTCTCTGGTGCCTTTCCTGTTACTGTGTACGGTGCTTTCCTACCCCGACATGTATTCTATCGTCTTCATGCTTTGTGTGCATACCTACGGTGCCTGTATGTTGCCCTCTGCGTGCTAGTCATGTGGCCGTCTTTTGATGTAATACTAGTAGATCAGGTTTCTGTTGTCATCCCATTGCTGAAGCTTAAGAAGTCGGCAAAGGTGTTGTTTTATTGTCATTTTCCAGATTTGTTGCTCGCTCAACACACAACTATTCTGAGGAGGATATATAGAAAACCCATAGACTTCATAGAAGAAATGACAACTGGAATGGCAGATAAGATTCTTGTTAACAGCAAATTTACAGCACTTACATTTGCAAAGACATTCAAGCATCTTGATGTACAAGGAATTAAGCCAGCTGTTCTTCACCCAGCTGTCAATGTGAATCAATTCGATGAACCTAGCAATTCTCCTAAGTTAAATTTTCTCTCCATCAACCGTTTTGAGAGGAAAAAGAATATAGACTTGGCAATTTCAGCTTTTGCCATGCTTCGCACCCTTGAAGGAGATATGCTTCAAGGTCTTAATCTGGCTGAAGCTTCCTTGACAATTGCAGGTGGCTTTGACAATCGGTTGAGAGAAAATGTTGAGTACTTGGAGGAGCTCAGAAGTTTAGCAGAAAGGGAAGGAGTGTCCAATCAGGTTAACTTCATCACATCTTGCTCAACAGCCGAAAGAAATGCACTACTCTCCCAGTGCCTATGTGTCGTTTATACACCAAAGGATGAACACTTTGGCATTGTTCCTCTGGAGGCAATGGCAGCTCATAAACCAGTTATTGCGTGCAACAGTGGGGGTCCTGTAGAGACAGTTAAGAATGGCGAAACGGGATTTCTTTGTGAAAGTAACCCAAGAGAGTTTTCCTTGGCTATGGCTAAACTCATTCAAGACCCACAGATGGCACAACGAATGGGTGTAGAAGCTCGGCGGCATGTTACAGAGTCATTCTCTACAAAAATATTCGGTCAGCATTTGAATCAATACCTAGTTGATGTTGCTCGGGCTAAAGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko00513 ko01100 map00510 map00513 map01100
Pfam Domains
Protein Families
Glyco_transf_4 Glycos_transf_1

Protein Analysis

410

Amino Acids

45.65

Weight (kDa)

7.64

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_4 PF13439 21 - 201 1.1e-13 Glycosyltransferase Family 4
Glyco_trans_4_4 PF13579 22 - 174 2.2e-06 Glycosyl transferase 4-like domain
GT4-conflict PF20706 187 - 345 6.1e-09 Family 4 Glycosyltransferase in conflict systems
Glycos_transf_1 PF00534 202 - 382 1.2e-36 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 214 - 369 1.7e-20 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 761
Acc36I ACCTGC 1 cut(s) 761
AccB1I GGYRCC 2 cut(s) 182, 264
AciI CCGC 2 cut(s) 92, 1144
AclI AACGTT 1 cut(s) 26
AcoI YGGCCR 2 cut(s) 115, 299
AcsI RAATTY 2 cut(s) 512, 637
AcuI CTGAAG 2 cut(s) 374, 771
AfaI GTAC 3 cut(s) 203, 558, 809
AfiI CCNNNNNNNGG 2 cut(s) 56, 1114
AflII CTTAAG 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 223
AgsI TTSAA 6 cut(s) 31, 544, 719, 734, 1103, 1192
AhlI ACTAGT 1 cut(s) 319
AjiI CACGTC 1 cut(s) 130
AjuI GAANNNNNNNTTGG 4 cut(s) 665, 697, 1060, 1092
Alw21I GWGCWC 2 cut(s) 105, 822
Alw26I GTCTC 3 cut(s) 164, 181, 1016
Ama87I CYCGRG 1 cut(s) 1215
AoxI GGCC 2 cut(s) 115, 299
ApeKI GCWGC 1 cut(s) 977
ApoI RAATTY 2 cut(s) 512, 637
Asp700I GAANNNNTTC 1 cut(s) 605
AspS9I GGNCC 1 cut(s) 1013
AsuHPI GGTGA 1 cut(s) 575
AvaI CYCGRG 1 cut(s) 1215
AvaII GGWCC 1 cut(s) 1013
BalI TGGCCA 1 cut(s) 117
BanI GGYRCC 2 cut(s) 182, 264
BanII GRGCYC 2 cut(s) 105, 822
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 2 cut(s) 105, 822
BbvI GCAGC 1 cut(s) 989
BccI CCATC 2 cut(s) 656, 1108
BceAI ACGGC 1 cut(s) 286
BcgI CGANNNNNNTGC 2 cut(s) 878, 912
BcoDI GTCTC 3 cut(s) 164, 181, 1016
BcuI ACTAGT 1 cut(s) 319
BfaI CTAG 4 cut(s) 290, 320, 618, 1202
BfmI CTRYAG 1 cut(s) 1017
BfrI CTTAAG 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 761
BisI GCNGC 3 cut(s) 93, 978, 1145
BlsI GCNGC 3 cut(s) 94, 979, 1146
BmcAI AGTACT 1 cut(s) 809
Bme18I GGWCC 1 cut(s) 1013
BmeT110I CYCGRG 1 cut(s) 1215
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 1013
BmiI GGNNCC 3 cut(s) 184, 266, 1014
BmrI ACTGGG 1 cut(s) 904
BmsI GCATC 4 cut(s) 79, 116, 149, 556
BmuI ACTGGG 1 cut(s) 904
BpiI GAAGAC 1 cut(s) 230
BplI GAGNNNNNCTC 4 cut(s) 161, 193, 1148, 1180
BpmI CTGGAG 1 cut(s) 986
Bsa29I ATCGAT 1 cut(s) 157
BsaBI GATNNNNATC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 1080
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 1114
Bse1I ACTGG 3 cut(s) 484, 910, 989
Bse3DI GCAATG 2 cut(s) 347, 978
Bse8I GATNNNNATC 1 cut(s) 153
BseCI ATCGAT 1 cut(s) 157
BseDI CCNNGG 1 cut(s) 1080
BseGI GGATG 4 cut(s) 46, 94, 342, 946
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 2 cut(s) 56, 1114
BseMI GCAATG 2 cut(s) 347, 978
BseMII CTCAG 2 cut(s) 422, 835
BseNI ACTGG 3 cut(s) 484, 910, 989
BseRI GAGGAG 5 cut(s) 27, 81, 182, 448, 830
BseXI GCAGC 1 cut(s) 989
BseYI CCCAGC 1 cut(s) 586
BshFI GGCC 2 cut(s) 117, 301
BshNI GGYRCC 2 cut(s) 182, 264
BshVI ATCGAT 1 cut(s) 157
BsiHKAI GWGCWC 2 cut(s) 105, 822
BsiHKCI CYCGRG 1 cut(s) 1215
BslI CCNNNNNNNGG 2 cut(s) 56, 1114
BsmAI GTCTC 3 cut(s) 164, 181, 1016
BsmBI CGTCTC 1 cut(s) 181
BsnI GGCC 2 cut(s) 117, 301
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 1215
Bsp1286I GDGCHC 2 cut(s) 105, 822
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 2 cut(s) 52, 325
BspACI CCGC 2 cut(s) 92, 1144
BspANI GGCC 2 cut(s) 117, 301
BspCNI CTCAG 2 cut(s) 423, 834
BspDI ATCGAT 1 cut(s) 157
BspLI GGNNCC 3 cut(s) 184, 266, 1014
BspMI ACCTGC 1 cut(s) 761
BspT107I GGYRCC 2 cut(s) 182, 264
BspTI CTTAAG 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 164
BsrDI GCAATG 2 cut(s) 347, 978
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 3 cut(s) 484, 910, 989
BssECI CCNNGG 1 cut(s) 1080
BssMI GATC 2 cut(s) 52, 325
BssT1I CCWWGG 1 cut(s) 1080
Bst4CI ACNGT 7 cut(s) 175, 199, 206, 264, 656, 1007, 1027
Bst6I CTCTTC 1 cut(s) 2
BstAFI CTTAAG 1 cut(s) 359
BstAUI TGTACA 1 cut(s) 556
BstC8I GCNNGC 5 cut(s) 105, 287, 411, 573, 1000
BstDEI CTNAG 3 cut(s) 431, 630, 821
BstF5I GGATG 4 cut(s) 46, 94, 342, 946
BstKTI GATC 2 cut(s) 55, 328
BstMAI GTCTC 3 cut(s) 164, 181, 1016
BstMBI GATC 2 cut(s) 52, 325
BstMWI GCNNNNNNNGC 4 cut(s) 109, 251, 692, 977
BstNSI RCATGY 2 cut(s) 227, 1151
BstSFI CTRYAG 1 cut(s) 1017
BstV1I GCAGC 1 cut(s) 989
BstV2I GAAGAC 1 cut(s) 230
Bsu15I ATCGAT 1 cut(s) 157
BsuRI GGCC 2 cut(s) 117, 301
BsuTUI ATCGAT 1 cut(s) 157
BtgZI GCGATG 1 cut(s) 93
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 4 cut(s) 46, 94, 342, 946
BtsIMutI CAGTG 2 cut(s) 917, 1012
BveI ACCTGC 1 cut(s) 761
Cac8I GCNNGC 5 cut(s) 105, 287, 411, 573, 1000
Cfr13I GGNCC 1 cut(s) 1013
ClaI ATCGAT 1 cut(s) 157
Csp6I GTAC 3 cut(s) 202, 557, 808
CviAII CATG 7 cut(s) 113, 146, 224, 242, 295, 703, 1148
CviQI GTAC 3 cut(s) 202, 557, 808
DdeI CTNAG 3 cut(s) 431, 630, 821
DpnI GATC 2 cut(s) 54, 327
DpnII GATC 2 cut(s) 52, 325
EaeI YGGCCR 2 cut(s) 115, 299
Eam1104I CTCTTC 1 cut(s) 2
EarI CTCTTC 1 cut(s) 2
Ecl136II GAGCTC 2 cut(s) 103, 820
Eco130I CCWWGG 1 cut(s) 1080
Eco24I GRGCYC 2 cut(s) 105, 822
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 1013
Eco53kI GAGCTC 2 cut(s) 103, 820
Eco57I CTGAAG 2 cut(s) 374, 771
Eco88I CYCGRG 1 cut(s) 1215
EcoICRI GAGCTC 2 cut(s) 103, 820
EcoO109I RGGNCCY 1 cut(s) 1013
EcoT14I CCWWGG 1 cut(s) 1080
EcoT38I GRGCYC 2 cut(s) 105, 822
ErhI CCWWGG 1 cut(s) 1080
Esp3I CGTCTC 1 cut(s) 181
FaeI CATG 7 cut(s) 116, 149, 227, 245, 298, 706, 1151
FatI CATG 7 cut(s) 112, 145, 223, 241, 294, 702, 1147
Fnu4HI GCNGC 3 cut(s) 93, 978, 1145
FokI GGATG 4 cut(s) 33, 101, 329, 953
FriOI GRGCYC 2 cut(s) 105, 822
Fsp4HI GCNGC 3 cut(s) 93, 978, 1145
FspBI CTAG 4 cut(s) 290, 320, 618, 1202
GluI GCNGC 3 cut(s) 93, 978, 1145
GsaI CCCAGC 1 cut(s) 590
GsuI CTGGAG 1 cut(s) 986
HaeIII GGCC 2 cut(s) 117, 301
Hin1II CATG 7 cut(s) 116, 149, 227, 245, 298, 706, 1151
HincII GTYRAC 2 cut(s) 505, 862
HindII GTYRAC 2 cut(s) 505, 862
HindIII AAGCTT 2 cut(s) 356, 753
HinfI GANTC 4 cut(s) 496, 601, 1157, 1192
HpaI GTTAAC 2 cut(s) 505, 862
HphI GGTGA 1 cut(s) 575
Hpy166II GTNNAC 4 cut(s) 127, 202, 505, 862
Hpy188I TCNGA 2 cut(s) 432, 824
Hpy188III TCNNGA 5 cut(s) 50, 398, 551, 965, 1103
Hpy8I GTNNAC 4 cut(s) 127, 202, 505, 862
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 713, 836
HpyCH4III ACNGT 7 cut(s) 175, 199, 206, 264, 656, 1007, 1027
HpyCH4IV ACGT 3 cut(s) 26, 33, 129
HpyCH4V TGCA 5 cut(s) 254, 533, 770, 899, 1002
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 251, 692, 977
HpyF3I CTNAG 3 cut(s) 431, 630, 821
HpySE526I ACGT 3 cut(s) 26, 33, 129
Hsp92II CATG 7 cut(s) 116, 149, 227, 245, 298, 706, 1151
KspAI GTTAAC 2 cut(s) 505, 862
Kzo9I GATC 2 cut(s) 52, 325
LmnI GCTCC 2 cut(s) 68, 817
Lsp1109I GCAGC 1 cut(s) 989
LweI GCATC 4 cut(s) 79, 116, 149, 556
MaeI CTAG 4 cut(s) 290, 320, 618, 1202
MaeII ACGT 3 cut(s) 26, 33, 129
MaeIII GTNAC 3 cut(s) 193, 1061, 1150
MalI GATC 2 cut(s) 54, 327
MboI GATC 2 cut(s) 52, 325
MboII GAAGA 4 cut(s) 19, 230, 479, 572
MfeI CAATTG 1 cut(s) 765
MhlI GDGCHC 2 cut(s) 105, 822
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 8 cut(s) 81, 512, 564, 605, 622, 637, 687, 765
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 1166
Mox20I TGGCCA 1 cut(s) 117
MroXI GAANNNNTTC 1 cut(s) 605
MscI TGGCCA 1 cut(s) 117
MseI TTAA 7 cut(s) 360, 504, 567, 635, 741, 861, 1029
MslI CAYNNNNRTG 1 cut(s) 552
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 2 cut(s) 575, 590
MspCI CTTAAG 1 cut(s) 359
MunI CAATTG 1 cut(s) 765
MwoI GCNNNNNNNGC 4 cut(s) 109, 251, 692, 977
NdeII GATC 2 cut(s) 52, 325
NlaIII CATG 7 cut(s) 116, 149, 227, 245, 298, 706, 1151
NlaIV GGNNCC 3 cut(s) 184, 266, 1014
NmeAIII GCCGAG 1 cut(s) 1120
NspI RCATGY 2 cut(s) 227, 1151
PaqCI CACCTGC 1 cut(s) 761
PciI ACATGT 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 3 cut(s) 496, 601, 1192
PkrI GCNGC 3 cut(s) 94, 979, 1146
PleI GAGTC 1 cut(s) 1165
PpsI GAGTC 1 cut(s) 1165
PpuMI RGGWCCY 1 cut(s) 1013
PscI ACATGT 1 cut(s) 223
Psp124BI GAGCTC 2 cut(s) 105, 822
Psp1406I AACGTT 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 1013
PspFI CCCAGC 1 cut(s) 586
PspN4I GGNNCC 3 cut(s) 184, 266, 1014
PspPI GGNCC 1 cut(s) 1013
PspPPI RGGWCCY 1 cut(s) 1013
PvuII CAGCTG 2 cut(s) 575, 590
RsaI GTAC 3 cut(s) 203, 558, 809
RsaNI GTAC 3 cut(s) 202, 557, 808
RseI CAYNNNNRTG 1 cut(s) 552
SacI GAGCTC 2 cut(s) 105, 822
SaqAI TTAA 7 cut(s) 360, 504, 567, 635, 741, 861, 1029
SatI GCNGC 3 cut(s) 93, 978, 1145
Sau3AI GATC 2 cut(s) 52, 325
Sau96I GGNCC 1 cut(s) 1013
ScaI AGTACT 1 cut(s) 809
SchI GAGTC 1 cut(s) 1166
SduI GDGCHC 2 cut(s) 105, 822
SfaNI GCATC 4 cut(s) 79, 116, 149, 556
SfcI CTRYAG 1 cut(s) 1017
SinI GGWCC 1 cut(s) 1013
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SpeI ACTAGT 1 cut(s) 319
Sse9I AATT 8 cut(s) 81, 512, 564, 605, 622, 637, 687, 765
SsiI CCGC 2 cut(s) 92, 1144
SspI AATATT 1 cut(s) 1176
SspMI CTAG 4 cut(s) 290, 320, 618, 1202
SstI GAGCTC 2 cut(s) 105, 822
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 7 cut(s) 175, 199, 206, 264, 656, 1007, 1027
TaiI ACGT 3 cut(s) 29, 36, 132
TaqI TCGA 2 cut(s) 157, 609
TaqII GACCGA 1 cut(s) 1169
TasI AATT 8 cut(s) 81, 512, 564, 605, 622, 637, 687, 765
TatI WGTACW 2 cut(s) 556, 807
TauI GCSGC 2 cut(s) 95, 1147
TfiI GAWTC 3 cut(s) 496, 601, 1192
Tru1I TTAA 7 cut(s) 360, 504, 567, 635, 741, 861, 1029
Tru9I TTAA 7 cut(s) 360, 504, 567, 635, 741, 861, 1029
TscAI CASTG 2 cut(s) 917, 1012
TseI GCWGC 1 cut(s) 977
TspDTI ATGAA 6 cut(s) 35, 230, 451, 627, 856, 957
TspRI CASTG 2 cut(s) 917, 1012
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 1013
XapI RAATTY 2 cut(s) 512, 637
XceI RCATGY 2 cut(s) 227, 1151
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 4 cut(s) 290, 320, 618, 1202
ZrmI AGTACT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.