pycom12g15920

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003984
Physical Location & Seq
Forward (+)
18332449 .. 18333491
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 885 bp
ATGTCCGTCGATTACTACAAGCTTCTTCAAGTTCAACGAAACGCCAGAGACGTGAGGAAAGCGGTCGAGGCCAAGCTCAATCTAATCAACGATGCCTACGAGACCGGCATGGCCCGGCTTACGTCTCAGGTTATGGAGTCCGTGAATCCGGAAGTGATATTCTCGACGTTTTTAGTCGGAGGGACGATAGGGGAAAATGGATTCCAGTGGTTTGAATGCACGGACTCAGGACCTGGAAATGGGCCGAGGAAAGCCGCGGCGATTGAGAGGACATCATTGCCTTGTACTTTGGAGGAACTGTATAAGGGCGCCACCAAGAAGCTGAAGATTTATAGGGATGTTCTTTGTGCTAGTGGAAGAAAAATCACAGTGGAGGAAGTTCTAACAATTGAGATCAAGCCAGGGTGGAAAAAGGGCACGAAAATCACTTTCCCAGAGAAAGGGCACGACACACGGCGAGGTGTGATACCAGCAGACATTGTCTTCATCGTCGATGAGAAGCCTCACGGTGTTTACAAGAGGGACGGGGACGATCTGATTGTCACTCAGACGATCTCTCTAGCGGAGGCTCTGACGGGTCACACCGCGCTGCTGACAGCCCTGGACGGGAGAAAATTGAGCGTCTCCATTGGTTCCATTATCAGCCAGACCCATGAAGAAGTGGTTATAGGGGAAGGGATGCCTATCCAGAAGGAACAATCCAAGAAGGGGAACTTGATTGTTAAGTTTAGCGTCAAGTTCCCCAAGCTCACCCCGGAGCAGAAAACCGGCATCAGAATGTTGCTTTTTATTTTTATTTTATTATTTTTTTATTTGGAAAACCTCGACGGTACGAGAGAAAAGCATCATAATGTTGCTGACATCTTTGTAAACGCCGGTTGTTGA

Protein Analysis

295

Amino Acids

32.67

Weight (kDa)

8.53

Isoelectric Point (pI)

26.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 91 - 248 1.1e-37 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccII CGCG 2 cut(s) 257, 587
AccIII TCCGGA 1 cut(s) 148
AciI CCGC 5 cut(s) 62, 255, 257, 563, 585
AcuI CTGAAG 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 2 cut(s) 286, 832
AfiI CCNNNNNNNGG 4 cut(s) 239, 440, 606, 708
AgsI TTSAA 3 cut(s) 29, 35, 215
AjiI CACGTC 1 cut(s) 52
AjnI CCWGG 3 cut(s) 232, 400, 600
AluBI AGCT 4 cut(s) 22, 76, 322, 748
AluI AGCT 4 cut(s) 22, 76, 322, 748
Alw26I GTCTC 4 cut(s) 42, 95, 129, 628
AlwNI CAGNNNCTG 1 cut(s) 233
Aor13HI TCCGGA 1 cut(s) 148
AoxI GGCC 3 cut(s) 69, 111, 242
ApeKI GCWGC 1 cut(s) 589
AspLEI GCGC 2 cut(s) 311, 589
AspS9I GGNCC 3 cut(s) 112, 230, 242
AsuC2I CCSGG 2 cut(s) 115, 755
AsuHPI GGTGA 1 cut(s) 742
AvaII GGWCC 1 cut(s) 230
BaeGI GKGCMC 2 cut(s) 419, 447
BanI GGYRCC 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 475
BbvI GCAGC 1 cut(s) 576
BceAI ACGGC 1 cut(s) 470
BciT130I CCWGG 3 cut(s) 234, 402, 602
BcnI CCSGG 2 cut(s) 115, 755
BcoDI GTCTC 4 cut(s) 42, 95, 129, 628
BfaI CTAG 2 cut(s) 351, 560
BfoI RGCGCY 1 cut(s) 312
BisI GCNGC 3 cut(s) 255, 258, 590
BlsI GCNGC 3 cut(s) 256, 259, 591
Bme1390I CCNGG 5 cut(s) 115, 234, 402, 602, 755
Bme18I GGWCC 1 cut(s) 230
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 3 cut(s) 112, 230, 242
BmiI GGNNCC 2 cut(s) 310, 634
BmrFI CCNGG 5 cut(s) 115, 234, 402, 602, 755
BmsI GCATC 4 cut(s) 82, 669, 780, 853
BpiI GAAGAC 1 cut(s) 475
BpuMI CCSGG 2 cut(s) 115, 755
BsaBI GATNNNNATC 1 cut(s) 683
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 5 cut(s) 245, 255, 401, 600, 753
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 440, 606, 708
Bse118I RCCGGY 3 cut(s) 104, 767, 875
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 275
Bse8I GATNNNNATC 1 cut(s) 683
BseAI TCCGGA 1 cut(s) 148
BseBI CCWGG 3 cut(s) 234, 402, 602
BseDI CCNNGG 5 cut(s) 245, 255, 401, 600, 753
BseGI GGATG 2 cut(s) 343, 684
BseJI GATNNNNATC 1 cut(s) 683
BseLI CCNNNNNNNGG 4 cut(s) 239, 440, 606, 708
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 3 cut(s) 140, 240, 560
BseNI ACTGG 1 cut(s) 205
BseSI GKGCMC 2 cut(s) 419, 447
BseXI GCAGC 1 cut(s) 576
Bsh1236I CGCG 2 cut(s) 257, 587
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 3 cut(s) 71, 113, 244
BshNI GGYRCC 1 cut(s) 308
BsiEI CGRYCG 1 cut(s) 66
BsiSI CCGG 6 cut(s) 105, 115, 149, 755, 768, 876
BslFI GGGAC 3 cut(s) 196, 536, 542
BslI CCNNNNNNNGG 4 cut(s) 239, 440, 606, 708
BsmAI GTCTC 4 cut(s) 42, 95, 129, 628
BsmBI CGTCTC 3 cut(s) 42, 129, 628
BsmFI GGGAC 3 cut(s) 196, 536, 542
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 3 cut(s) 71, 113, 244
Bso31I GGTCTC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 419, 447
Bsp13I TCCGGA 1 cut(s) 148
Bsp143I GATC 3 cut(s) 393, 532, 552
BspACI CCGC 5 cut(s) 62, 255, 257, 563, 585
BspANI GGCC 3 cut(s) 71, 113, 244
BspCNI CTCAG 3 cut(s) 139, 239, 559
BspEI TCCGGA 1 cut(s) 148
BspFNI CGCG 2 cut(s) 257, 587
BspLI GGNNCC 2 cut(s) 310, 634
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 275
BsrFI RCCGGY 3 cut(s) 104, 767, 875
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 3 cut(s) 104, 767, 875
BssECI CCNNGG 5 cut(s) 245, 255, 401, 600, 753
BssMI GATC 3 cut(s) 393, 532, 552
BssNI GRCGYC 1 cut(s) 309
Bst2UI CCWGG 3 cut(s) 234, 402, 602
Bst4CI ACNGT 4 cut(s) 300, 370, 509, 830
BstACI GRCGYC 1 cut(s) 309
BstDEI CTNAG 3 cut(s) 126, 226, 546
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 2 cut(s) 343, 684
BstFNI CGCG 2 cut(s) 257, 587
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 2 cut(s) 311, 589
BstKTI GATC 3 cut(s) 396, 535, 555
BstMAI GTCTC 4 cut(s) 42, 95, 129, 628
BstMBI GATC 3 cut(s) 393, 532, 552
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 3 cut(s) 234, 402, 602
BstSCI CCNGG 5 cut(s) 113, 232, 400, 600, 753
BstSLI GKGCMC 2 cut(s) 419, 447
BstUI CGCG 2 cut(s) 257, 587
BstV1I GCAGC 1 cut(s) 576
BstV2I GAAGAC 1 cut(s) 475
BsuRI GGCC 3 cut(s) 71, 113, 244
BtgI CCRYGG 1 cut(s) 255
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 2 cut(s) 343, 684
BtsIMutI CAGTG 2 cut(s) 212, 375
CaiI CAGNNNCTG 1 cut(s) 233
CfoI GCGC 2 cut(s) 311, 589
Cfr10I RCCGGY 3 cut(s) 104, 767, 875
Cfr13I GGNCC 3 cut(s) 112, 230, 242
Cfr42I CCGCGG 1 cut(s) 258
CseI GACGC 2 cut(s) 610, 721
Csp6I GTAC 2 cut(s) 285, 831
CviAII CATG 2 cut(s) 109, 653
CviQI GTAC 2 cut(s) 285, 831
DdeI CTNAG 3 cut(s) 126, 226, 546
DinI GGCGCC 1 cut(s) 310
DpnI GATC 3 cut(s) 395, 534, 554
DpnII GATC 3 cut(s) 393, 532, 552
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 230
EcoRII CCWGG 3 cut(s) 232, 400, 600
EgeI GGCGCC 1 cut(s) 310
EheI GGCGCC 1 cut(s) 310
Esp3I CGTCTC 3 cut(s) 42, 129, 628
FaeI CATG 2 cut(s) 112, 656
FaiI YATR 7 cut(s) 110, 134, 303, 333, 654, 668, 849
FalI AAGNNNNNCTT 2 cut(s) 698, 730
FaqI GGGAC 3 cut(s) 196, 536, 542
FatI CATG 2 cut(s) 108, 652
Fnu4HI GCNGC 3 cut(s) 255, 258, 590
FokI GGATG 2 cut(s) 350, 691
Fsp4HI GCNGC 3 cut(s) 255, 258, 590
FspBI CTAG 2 cut(s) 351, 560
GlaI GCGC 2 cut(s) 310, 588
GluI GCNGC 3 cut(s) 255, 258, 590
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 3 cut(s) 71, 113, 244
HapII CCGG 6 cut(s) 105, 115, 149, 755, 768, 876
HgaI GACGC 2 cut(s) 610, 721
HhaI GCGC 2 cut(s) 311, 589
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 2 cut(s) 112, 656
Hin6I GCGC 2 cut(s) 309, 587
HinP1I GCGC 2 cut(s) 309, 587
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 4 cut(s) 137, 145, 201, 224
HpaII CCGG 6 cut(s) 105, 115, 149, 755, 768, 876
HphI GGTGA 1 cut(s) 742
Hpy166II GTNNAC 2 cut(s) 514, 871
Hpy188I TCNGA 5 cut(s) 179, 537, 549, 573, 776
Hpy188III TCNNGA 4 cut(s) 149, 163, 228, 688
Hpy8I GTNNAC 2 cut(s) 514, 871
Hpy99I CGWCG 4 cut(s) 11, 169, 494, 830
HpyAV CCTTC 3 cut(s) 668, 685, 700
HpyCH4III ACNGT 4 cut(s) 300, 370, 509, 830
HpyCH4IV ACGT 3 cut(s) 51, 122, 167
HpyCH4V TGCA 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 3 cut(s) 126, 226, 546
HpySE526I ACGT 3 cut(s) 51, 122, 167
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 2 cut(s) 112, 656
HspAI GCGC 2 cut(s) 309, 587
KasI GGCGCC 1 cut(s) 308
Kpn2I TCCGGA 1 cut(s) 148
KspI CCGCGG 1 cut(s) 258
Kzo9I GATC 3 cut(s) 393, 532, 552
LmnI GCTCC 1 cut(s) 757
Lsp1109I GCAGC 1 cut(s) 576
LweI GCATC 4 cut(s) 82, 669, 780, 853
MaeI CTAG 2 cut(s) 351, 560
MaeII ACGT 3 cut(s) 51, 122, 167
MaeIII GTNAC 2 cut(s) 541, 578
MalI GATC 3 cut(s) 395, 534, 554
MboI GATC 3 cut(s) 393, 532, 552
MboII GAAGA 5 cut(s) 17, 337, 369, 475, 668
MfeI CAATTG 1 cut(s) 387
MhlI GDGCHC 2 cut(s) 419, 447
MluCI AATT 2 cut(s) 387, 614
Mly113I GGCGCC 1 cut(s) 309
MlyI GAGTC 2 cut(s) 146, 218
MmeI TCCRAC 1 cut(s) 157
MroI TCCGGA 1 cut(s) 148
MseI TTAA 1 cut(s) 723
MslI CAYNNNNRTG 2 cut(s) 776, 849
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 6 cut(s) 105, 115, 149, 755, 768, 876
MspR9I CCNGG 5 cut(s) 115, 234, 402, 602, 755
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 3 cut(s) 234, 402, 602
MvnI CGCG 2 cut(s) 257, 587
MwoI GCNNNNNNNGC 1 cut(s) 68
NarI GGCGCC 1 cut(s) 309
NciI CCSGG 2 cut(s) 115, 755
NdeII GATC 3 cut(s) 393, 532, 552
NlaIII CATG 2 cut(s) 112, 656
NlaIV GGNNCC 2 cut(s) 310, 634
NmeAIII GCCGAG 1 cut(s) 270
NmuCI GTSAC 2 cut(s) 541, 578
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 96
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 145, 201
PflFI GACNNNGTC 1 cut(s) 479
PkrI GCNGC 3 cut(s) 256, 259, 591
PleI GAGTC 2 cut(s) 145, 218
PluTI GGCGCC 1 cut(s) 312
PpsI GAGTC 2 cut(s) 145, 218
PpuMI RGGWCCY 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 230
Psp6I CCWGG 3 cut(s) 232, 400, 600
PspGI CCWGG 3 cut(s) 232, 400, 600
PspN4I GGNNCC 2 cut(s) 310, 634
PspPI GGNCC 3 cut(s) 112, 230, 242
PspPPI RGGWCCY 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 233
PsyI GACNNNGTC 1 cut(s) 479
RsaI GTAC 2 cut(s) 286, 832
RsaNI GTAC 2 cut(s) 285, 831
RseI CAYNNNNRTG 2 cut(s) 776, 849
SacII CCGCGG 1 cut(s) 258
SaqAI TTAA 1 cut(s) 723
SatI GCNGC 3 cut(s) 255, 258, 590
Sau3AI GATC 3 cut(s) 393, 532, 552
Sau96I GGNCC 3 cut(s) 112, 230, 242
SchI GAGTC 2 cut(s) 146, 218
ScrFI CCNGG 5 cut(s) 115, 234, 402, 602, 755
SduI GDGCHC 2 cut(s) 419, 447
SfaNI GCATC 4 cut(s) 82, 669, 780, 853
SfoI GGCGCC 1 cut(s) 310
Sfr303I CCGCGG 1 cut(s) 258
SgrBI CCGCGG 1 cut(s) 258
SinI GGWCC 1 cut(s) 230
SmiMI CAYNNNNRTG 2 cut(s) 776, 849
Sse9I AATT 2 cut(s) 387, 614
SsiI CCGC 5 cut(s) 62, 255, 257, 563, 585
SspDI GGCGCC 1 cut(s) 308
SspMI CTAG 2 cut(s) 351, 560
StyD4I CCNGG 5 cut(s) 113, 232, 400, 600, 753
TaaI ACNGT 4 cut(s) 300, 370, 509, 830
TaiI ACGT 3 cut(s) 54, 125, 170
TaqI TCGA 5 cut(s) 9, 66, 164, 492, 825
TasI AATT 2 cut(s) 387, 614
TatI WGTACW 1 cut(s) 284
TauI GCSGC 2 cut(s) 257, 260
TfiI GAWTC 2 cut(s) 145, 201
Tru1I TTAA 1 cut(s) 723
Tru9I TTAA 1 cut(s) 723
TscAI CASTG 2 cut(s) 212, 375
TseFI GTSAC 2 cut(s) 541, 578
TseI GCWGC 1 cut(s) 589
Tsp45I GTSAC 2 cut(s) 541, 578
TspDTI ATGAA 2 cut(s) 475, 669
TspGWI ACGGA 2 cut(s) 130, 236
TspRI CASTG 2 cut(s) 212, 375
Tth111I GACNNNGTC 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 230
XspI CTAG 2 cut(s) 351, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.