pycom12g18100
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001828
Physical Location & Seq
Forward (+)
20361815 .. 20364847
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1080 bp
ATGGAAGTGGGAAGGAGAATATCGGCAAGTCCTCGTCCGTGCTCCGTCCGGCGAGTGGTGGCGAAGAAGAGGGGTGGCTCCGATGGGTTTGTAAACAGCGTGAAGAAGCTTCAGAGGCGTGAGATTTCTTCGAAGCGTGATCGAGCTTTTGGGATGAGCAATTCCCAGGAGAGGTTCCGCAACATGCACCTCGTGGAGGAATATGATACTCATGACCCCAAGGGACATTCTTCGGCCATATTGCCTTTTCTGATGAAAAGGACAAAGGTCATTGAGATAGTTGCCGCACGTGACATCGTCTTTGCTCTCGCACACTCTGGTGTTTGCGCAGCTTTTAGTAGAGAGAAAAACGAGAGGATTTGTTTTATGAATGTTAGTCCTGATGAAGTCATTCGGAGCTTGTTCTATAATAAGAACAACGATTCGCTCATCACAGTTTCAGTTTATGCTTCAGACAACTTCAGCTCTTTGAAATGCAGATCCACAAGGATTGAATATATAAGGAGGGGACAGCCAGAAGCTGGCTTTGCTCTTTTCGAGTCCGAGTCTTTGAAGTGGCCTGGGTTTGTAGAGTTTGATGATGTTAATGGCAAGGTGCTGACATACTCAGCACAGGATAGTATATACAAGGTGTTTGACCTTAAAAACTATACCATGCTGTACTCTATATCAGACAAACATGTACAAGAAATCAAAATCAGTCCAGGCATCATGCTGTTGATTTATAACAGAGCTACTAGCCATGTTCCTCTGAAGATTCTATCAATAGAAGATGGCACGGTTCTCAAAGACTTCAATCATCTACTTCATAGAAATAAGAAGGTGGACTTCATAGAACAGTTCAATGAAAAGCTTCTTGTCAAGCAAGAAAATGAGAACCTTCAGATTCTGGATGTTCGAAATGCTGAGATGATGGAAGTTAGCAGAACAGACTTCATAACTCCATCAGCATTTATCTTTCTGTATGAGAACCAGTTATTCCTGACGTTCAGAAATCGATTGGTGTCTGTGTGGAACTTTCGTGGAGAACTTGTAACTGCGTTTGAGGATCGATCCCCCGAACTTGTTATGGCATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

360

Amino Acids

41.4

Weight (kDa)

9.11

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7899 PF25463 48 - 351 7.1e-175 Domain of unknown function (DUF7899)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
Acc16I TGCGCA 1 cut(s) 328
AccB7I CCANNNNNTGG 1 cut(s) 521
AciI CCGC 2 cut(s) 178, 285
AclWI GGATC 3 cut(s) 474, 1047, 1056
AcoI YGGCCR 1 cut(s) 234
AcuI CTGAAG 5 cut(s) 95, 435, 445, 773, 866
AcvI CACGTG 1 cut(s) 290
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 2 cut(s) 662, 684
AfiI CCNNNNNNNGG 4 cut(s) 55, 171, 196, 521
AflIII ACRYGT 1 cut(s) 679
AgsI TTSAA 5 cut(s) 472, 494, 553, 796, 844
AjnI CCWGG 3 cut(s) 165, 559, 703
AleI CACNNNNGTG 1 cut(s) 318
AluBI AGCT 8 cut(s) 109, 146, 332, 399, 465, 521, 734, 853
AluI AGCT 8 cut(s) 109, 146, 332, 399, 465, 521, 734, 853
Alw21I GWGCWC 1 cut(s) 44
AlwI GGATC 3 cut(s) 474, 1047, 1056
AlwNI CAGNNNCTG 2 cut(s) 521, 889
AoxI GGCC 2 cut(s) 234, 557
ApeKI GCWGC 1 cut(s) 329
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
Asp700I GAANNNNTTC 2 cut(s) 390, 852
AspLEI GCGC 1 cut(s) 329
AsuII TTCGAA 2 cut(s) 131, 898
BauI CACGAG 1 cut(s) 191
BbrPI CACGTG 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 341
BccI CCATC 4 cut(s) 77, 767, 907, 952
BciT130I CCWGG 3 cut(s) 167, 561, 705
BfaI CTAG 1 cut(s) 738
BisI GCNGC 2 cut(s) 285, 330
BlsI GCNGC 2 cut(s) 286, 331
Bme1390I CCNGG 3 cut(s) 167, 561, 705
BmiI GGNNCC 2 cut(s) 79, 176
BmrFI CCNGG 3 cut(s) 167, 561, 705
BmsI GCATC 1 cut(s) 717
BoxI GACNNNNGTC 1 cut(s) 266
Bpu14I TTCGAA 2 cut(s) 131, 898
Bsa29I ATCGAT 2 cut(s) 997, 1051
BsaAI YACGTR 1 cut(s) 290
BsaJI CCNNGG 3 cut(s) 165, 219, 560
BsaXI ACNNNNNCTCC 2 cut(s) 1017, 1047
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 171, 196, 521
Bse1I ACTGG 1 cut(s) 973
BseBI CCWGG 3 cut(s) 167, 561, 705
BseCI ATCGAT 2 cut(s) 997, 1051
BseDI CCNNGG 3 cut(s) 165, 219, 560
BseGI GGATG 3 cut(s) 159, 898, 1073
BseLI CCNNNNNNNGG 4 cut(s) 55, 171, 196, 521
BseMII CTCAG 2 cut(s) 621, 897
BseNI ACTGG 1 cut(s) 973
BseXI GCAGC 1 cut(s) 341
BshFI GGCC 2 cut(s) 236, 559
BshVI ATCGAT 2 cut(s) 997, 1051
BsiHKAI GWGCWC 1 cut(s) 44
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 2 cut(s) 237, 522
BslI CCNNNNNNNGG 4 cut(s) 55, 171, 196, 521
BsmFI GGGAC 2 cut(s) 237, 522
BsnI GGCC 2 cut(s) 236, 559
Bsp119I TTCGAA 2 cut(s) 131, 898
Bsp1286I GDGCHC 1 cut(s) 44
Bsp1407I TGTACA 1 cut(s) 682
Bsp143I GATC 4 cut(s) 139, 479, 1048, 1052
BspACI CCGC 2 cut(s) 178, 285
BspANI GGCC 2 cut(s) 236, 559
BspCNI CTCAG 2 cut(s) 620, 898
BspDI ATCGAT 2 cut(s) 997, 1051
BspHI TCATGA 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 79, 176
BspPI GGATC 3 cut(s) 474, 1047, 1056
BspT104I TTCGAA 2 cut(s) 131, 898
BsrGI TGTACA 1 cut(s) 682
BsrI ACTGG 1 cut(s) 973
BssECI CCNNGG 3 cut(s) 165, 219, 560
BssMI GATC 4 cut(s) 139, 479, 1048, 1052
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 219
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 3 cut(s) 167, 561, 705
Bst4CI ACNGT 3 cut(s) 436, 781, 840
Bst6I CTCTTC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 682
BstBAI YACGTR 1 cut(s) 290
BstBI TTCGAA 2 cut(s) 131, 898
BstC8I GCNNGC 1 cut(s) 523
BstDEI CTNAG 2 cut(s) 607, 906
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 3 cut(s) 159, 898, 1073
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 4 cut(s) 142, 482, 1051, 1055
BstMBI GATC 4 cut(s) 139, 479, 1048, 1052
BstMWI GCNNNNNNNGC 2 cut(s) 115, 527
BstNI CCWGG 3 cut(s) 167, 561, 705
BstNSI RCATGY 2 cut(s) 187, 683
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 3 cut(s) 165, 559, 703
BstV1I GCAGC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
Bsu15I ATCGAT 2 cut(s) 997, 1051
BsuRI GGCC 2 cut(s) 236, 559
BsuTUI ATCGAT 2 cut(s) 997, 1051
BtsCI GGATG 3 cut(s) 159, 898, 1073
Cac8I GCNNGC 1 cut(s) 523
CaiI CAGNNNCTG 2 cut(s) 521, 889
CciI TCATGA 1 cut(s) 211
CfoI GCGC 1 cut(s) 329
ClaI ATCGAT 2 cut(s) 997, 1051
Csp6I GTAC 2 cut(s) 661, 683
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviAII CATG 6 cut(s) 184, 212, 655, 680, 712, 743
CviQI GTAC 2 cut(s) 661, 683
DdeI CTNAG 2 cut(s) 607, 906
DpnI GATC 4 cut(s) 141, 481, 1050, 1054
DpnII GATC 4 cut(s) 139, 479, 1048, 1052
DraIII CACNNNGTG 1 cut(s) 193
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 219
Eco57I CTGAAG 5 cut(s) 95, 435, 445, 773, 866
Eco72I CACGTG 1 cut(s) 290
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoRII CCWGG 3 cut(s) 165, 559, 703
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 6 cut(s) 187, 215, 658, 683, 715, 746
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FaqI GGGAC 2 cut(s) 237, 522
FatI CATG 6 cut(s) 183, 211, 654, 679, 711, 742
Fnu4HI GCNGC 2 cut(s) 285, 330
FokI GGATG 3 cut(s) 166, 905, 1060
Fsp4HI GCNGC 2 cut(s) 285, 330
FspBI CTAG 1 cut(s) 738
FspI TGCGCA 1 cut(s) 328
GlaI GCGC 1 cut(s) 328
GluI GCNGC 2 cut(s) 285, 330
HaeIII GGCC 2 cut(s) 236, 559
HapII CCGG 1 cut(s) 49
HhaI GCGC 1 cut(s) 329
Hin1II CATG 6 cut(s) 187, 215, 658, 683, 715, 746
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HindIII AAGCTT 2 cut(s) 107, 851
HinfI GANTC 5 cut(s) 422, 539, 545, 757, 886
HpaII CCGG 1 cut(s) 49
Hpy166II GTNNAC 2 cut(s) 94, 826
Hpy188III TCNNGA 5 cut(s) 212, 380, 890, 982, 1077
Hpy8I GTNNAC 2 cut(s) 94, 826
HpyAV CCTTC 3 cut(s) 6, 814, 890
HpyCH4III ACNGT 3 cut(s) 436, 781, 840
HpyCH4IV ACGT 2 cut(s) 289, 986
HpyCH4V TGCA 2 cut(s) 187, 477
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 527
HpyF3I CTNAG 2 cut(s) 607, 906
HpySE526I ACGT 2 cut(s) 289, 986
Hsp92II CATG 6 cut(s) 187, 215, 658, 683, 715, 746
HspAI GCGC 1 cut(s) 327
Kzo9I GATC 4 cut(s) 139, 479, 1048, 1052
LmnI GCTCC 3 cut(s) 47, 83, 396
Lsp1109I GCAGC 1 cut(s) 341
LweI GCATC 1 cut(s) 717
MaeI CTAG 1 cut(s) 738
MaeII ACGT 2 cut(s) 289, 986
MaeIII GTNAC 2 cut(s) 290, 1033
MalI GATC 4 cut(s) 141, 481, 1050, 1054
MboI GATC 4 cut(s) 139, 479, 1048, 1052
MboII GAAGA 7 cut(s) 76, 79, 115, 120, 222, 766, 782
MflI RGATCY 1 cut(s) 479
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 1 cut(s) 160
MlyI GAGTC 2 cut(s) 548, 554
MroXI GAANNNNTTC 2 cut(s) 390, 852
MseI TTAA 2 cut(s) 585, 642
MslI CAYNNNNRTG 1 cut(s) 318
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 3 cut(s) 167, 561, 705
MvaI CCWGG 3 cut(s) 167, 561, 705
MwoI GCNNNNNNNGC 2 cut(s) 115, 527
NdeII GATC 4 cut(s) 139, 479, 1048, 1052
NlaIII CATG 6 cut(s) 187, 215, 658, 683, 715, 746
NlaIV GGNNCC 2 cut(s) 79, 176
NmuCI GTSAC 1 cut(s) 290
NsbI TGCGCA 1 cut(s) 328
NspI RCATGY 2 cut(s) 187, 683
NspV TTCGAA 2 cut(s) 131, 898
OliI CACNNNNGTG 1 cut(s) 318
PagI TCATGA 1 cut(s) 211
PciI ACATGT 1 cut(s) 679
PdmI GAANNNNTTC 2 cut(s) 390, 852
PfeI GAWTC 3 cut(s) 422, 757, 886
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 521
PkrI GCNGC 2 cut(s) 286, 331
PleI GAGTC 2 cut(s) 547, 553
PmaCI CACGTG 1 cut(s) 290
PmlI CACGTG 1 cut(s) 290
PpsI GAGTC 2 cut(s) 547, 553
Ppu21I YACGTR 1 cut(s) 290
PscI ACATGT 1 cut(s) 679
PshAI GACNNNNGTC 1 cut(s) 266
PsiI TTATAA 1 cut(s) 726
Psp6I CCWGG 3 cut(s) 165, 559, 703
PspCI CACGTG 1 cut(s) 290
PspGI CCWGG 3 cut(s) 165, 559, 703
PspN4I GGNNCC 2 cut(s) 79, 176
PstNI CAGNNNCTG 2 cut(s) 521, 889
PsuI RGATCY 1 cut(s) 479
PsyI GACNNNGTC 1 cut(s) 296
RsaI GTAC 2 cut(s) 662, 684
RsaNI GTAC 2 cut(s) 661, 683
RseI CAYNNNNRTG 1 cut(s) 318
SaqAI TTAA 2 cut(s) 585, 642
SatI GCNGC 2 cut(s) 285, 330
Sau3AI GATC 4 cut(s) 139, 479, 1048, 1052
SchI GAGTC 2 cut(s) 548, 554
ScrFI CCNGG 3 cut(s) 167, 561, 705
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 1 cut(s) 717
SfuI TTCGAA 2 cut(s) 131, 898
SmiMI CAYNNNNRTG 1 cut(s) 318
Sse9I AATT 1 cut(s) 160
SsiI CCGC 2 cut(s) 178, 285
SspMI CTAG 1 cut(s) 738
StyD4I CCNGG 3 cut(s) 165, 559, 703
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 3 cut(s) 436, 781, 840
TaiI ACGT 2 cut(s) 292, 989
TaqI TCGA 6 cut(s) 131, 142, 537, 898, 997, 1051
TasI AATT 1 cut(s) 160
TatI WGTACW 2 cut(s) 660, 682
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 3 cut(s) 422, 757, 886
Tru1I TTAA 2 cut(s) 585, 642
Tru9I TTAA 2 cut(s) 585, 642
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 7 cut(s) 269, 383, 399, 797, 820, 861, 925
TspGWI ACGGA 2 cut(s) 27, 34
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 1 cut(s) 521
XagI CCTNNNNNAGG 1 cut(s) 194
XceI RCATGY 2 cut(s) 187, 683
XmnI GAANNNNTTC 2 cut(s) 390, 852
XspI CTAG 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.