pycom13g06510

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004201
Physical Location & Seq
Reverse (-)
4221875 .. 4225043
3169 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1134 bp
ATGTTTATCAAACGAGTCGATTTAAAAACCTTATCCCTCTGCTCAAACAGTCGTCTCTCCCGCTGCTCTGCAATTCTCTGCATGCTCAAAAGTTCGAATACAATATCAAAACCAGCAATGGTGTGCACGCTGTTCACATCTGTAGCTCAATCTTCCTTCTCCTTCTTATCAGACTCACCCAAGCCAACTTCCTCCAAAACCCAAATCCCCTCCCTTTTTCTCGACGGTCGCAGAAGCTTCACCGCCGGCTTCTCGCCGAGCCGATCGACCCAGCTTTCATGGCCCAGAAACGTCAGAAATCCGCCGTACATTTGCTTTTTCAGCGGCGGAGGAAAGCCCAAAGGCGATTTCCAAGAAAAGGAAAATGAGTCGCAGTGGCGGATTTTGAAGCGGTGGGATGTGCCCTGGGAATGGCAAACGGCGTCGTTAACATCGCTTGCTTGTGGGCTGAGTGTTTATAACCGTGCTTTGCGAATTTCGACATGGTCTGACTGGTTTGGTTATTGTTTCGCTAGTATTACAACTGCAGCTGTACTTGCTGTTCTATATACCGTTGCCAACACTTACCAACCACTGCCTGAAGATGTTTATCGCTACGATTTGAAGGAACCTTTCAATCTACAGAAGGGATGGCTATTGTGGGCAGGGATTGGTTTTGTTGGTGCCATAGCTGCTATTGCAGTGACAGGGGCTGCATTGTCCTTGTTGAGGGGGGAGAGCCCAGAACGAGAGAAGGACGCTCTCGTAAGTTTGCTTCCACTGATTGGATCTTCCGGTATTAGCACTGCTTGCTTGCTAGGCATCACTGGAGTTTTGGCTCCAATTCTTGAAGAAACTGTGTTTCGTGGGTTTTTTATGGTCTCCATGACTAAATGGGTACCTACACCAGTGGCTATTGTAATAAGTGCAGCTGTATTTGCATTCGCACATCTCACTCCTGGAGAATTTCCCCAGCTGTTTGTGCTAGGCACTGCTCTGGGATTGTCATATGCTCAAACTCGCAACCTTGTAACTCCCATCACAATACATGCTCTCTGGAACTCTTGGAGTCCTCTGATCGATCTTGCTTCTGACCTTCCTTCAGAGGATTGCTGCAAGGCTTGCTTAAAAGTCGTCAAAAATCAGGATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

378

Amino Acids

41.37

Weight (kDa)

8.93

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 263 - 348 2.6e-20 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
Acc65I GGTACC 1 cut(s) 877
AccB1I GGYRCC 2 cut(s) 662, 877
AccB7I CCANNNNNTGG 1 cut(s) 764
AciI CCGC 7 cut(s) 61, 243, 302, 324, 327, 379, 391
AclWI GGATC 1 cut(s) 775
AcsI RAATTY 2 cut(s) 474, 944
AcuI CTGAAG 2 cut(s) 600, 1065
AcyI GRCGYC 1 cut(s) 422
AfaI GTAC 3 cut(s) 308, 534, 879
AfiI CCNNNNNNNGG 4 cut(s) 358, 411, 708, 764
AgsI TTSAA 4 cut(s) 388, 604, 616, 830
AjnI CCWGG 2 cut(s) 404, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 7 cut(s) 146, 237, 274, 530, 671, 911, 955
AluI AGCT 7 cut(s) 146, 237, 274, 530, 671, 911, 955
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 2 cut(s) 59, 865
Alw44I GTGCAC 1 cut(s) 124
AlwI GGATC 1 cut(s) 775
AlwNI CAGNNNCTG 1 cut(s) 692
AoxI GGCC 1 cut(s) 281
ApaLI GTGCAC 1 cut(s) 124
ApeKI GCWGC 6 cut(s) 63, 527, 671, 692, 908, 1092
ApoI RAATTY 2 cut(s) 474, 944
Asp718I GGTACC 1 cut(s) 877
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 168, 232
AsuII TTCGAA 1 cut(s) 95
BaeGI GKGCMC 2 cut(s) 128, 405
BanI GGYRCC 2 cut(s) 662, 877
BanII GRGCYC 1 cut(s) 722
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 6 cut(s) 50, 539, 658, 679, 920, 1079
BccI CCATC 2 cut(s) 624, 1025
BceAI ACGGC 2 cut(s) 289, 435
BciT130I CCWGG 2 cut(s) 406, 939
BcoDI GTCTC 2 cut(s) 59, 865
BfaI CTAG 3 cut(s) 513, 797, 965
BfmI CTRYAG 3 cut(s) 141, 525, 620
BisI GCNGC 7 cut(s) 64, 325, 528, 672, 693, 909, 1093
BlsI GCNGC 7 cut(s) 65, 326, 529, 673, 694, 910, 1094
Bme1390I CCNGG 2 cut(s) 406, 939
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 4 cut(s) 609, 664, 819, 879
BmrFI CCNGG 2 cut(s) 406, 939
BmsI GCATC 1 cut(s) 810
BpmI CTGGAG 2 cut(s) 828, 960
Bpu14I TTCGAA 1 cut(s) 95
Bsa29I ATCGAT 1 cut(s) 1059
BsaBI GATNNNNATC 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 865
BsaJI CCNNGG 2 cut(s) 404, 405
BsaWI WCCGGW 1 cut(s) 773
Bsc4I CCNNNNNNNGG 4 cut(s) 358, 411, 708, 764
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 3 cut(s) 497, 811, 887
Bse3DI GCAATG 1 cut(s) 123
Bse8I GATNNNNATC 1 cut(s) 588
BseBI CCWGG 2 cut(s) 406, 939
BseCI ATCGAT 1 cut(s) 1059
BseDI CCNNGG 2 cut(s) 404, 405
BseGI GGATG 2 cut(s) 403, 635
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 4 cut(s) 358, 411, 708, 764
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 3 cut(s) 497, 811, 887
BseSI GKGCMC 2 cut(s) 128, 405
BseXI GCAGC 6 cut(s) 50, 539, 658, 679, 920, 1079
BseYI CCCAGC 2 cut(s) 270, 951
BsgI GTGCAG 1 cut(s) 927
Bsh1285I CGRYCG 2 cut(s) 229, 266
BshFI GGCC 1 cut(s) 283
BshNI GGYRCC 2 cut(s) 662, 877
BshVI ATCGAT 1 cut(s) 1059
BsiEI CGRYCG 2 cut(s) 229, 266
BsiHKAI GWGCWC 1 cut(s) 128
BsiSI CCGG 2 cut(s) 246, 774
BslI CCNNNNNNNGG 4 cut(s) 358, 411, 708, 764
BsmAI GTCTC 2 cut(s) 59, 865
BsmBI CGTCTC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 920
BsnI GGCC 1 cut(s) 283
Bso31I GGTCTC 1 cut(s) 865
Bsp119I TTCGAA 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 128, 405, 722
Bsp143I GATC 5 cut(s) 263, 767, 1056, 1060, 1126
BspACI CCGC 7 cut(s) 61, 243, 302, 324, 327, 379, 391
BspANI GGCC 1 cut(s) 283
BspCNI CTCAG 1 cut(s) 441
BspDI ATCGAT 1 cut(s) 1059
BspLI GGNNCC 4 cut(s) 609, 664, 819, 879
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 1 cut(s) 775
BspT104I TTCGAA 1 cut(s) 95
BspT107I GGYRCC 2 cut(s) 662, 877
BspTNI GGTCTC 1 cut(s) 865
BsrDI GCAATG 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 3 cut(s) 497, 811, 887
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 404, 405
BssMI GATC 5 cut(s) 263, 767, 1056, 1060, 1126
BssNI GRCGYC 1 cut(s) 422
Bst2UI CCWGG 2 cut(s) 406, 939
Bst4CI ACNGT 5 cut(s) 50, 227, 464, 553, 838
BstACI GRCGYC 1 cut(s) 422
BstAPI GCANNNNNTGC 2 cut(s) 789, 1101
BstBI TTCGAA 1 cut(s) 95
BstC8I GCNNGC 7 cut(s) 83, 128, 247, 438, 790, 794, 1102
BstDEI CTNAG 1 cut(s) 449
BstENI CCTNNNNNAGG 1 cut(s) 706
BstF5I GGATG 2 cut(s) 403, 635
BstKTI GATC 5 cut(s) 266, 770, 1059, 1063, 1129
BstMAI GTCTC 2 cut(s) 59, 865
BstMBI GATC 5 cut(s) 263, 767, 1056, 1060, 1126
BstMCI CGRYCG 2 cut(s) 229, 266
BstNI CCWGG 2 cut(s) 406, 939
BstNSI RCATGY 2 cut(s) 85, 1031
BstSCI CCNGG 2 cut(s) 404, 937
BstSFI CTRYAG 3 cut(s) 141, 525, 620
BstSLI GKGCMC 2 cut(s) 128, 405
BstV1I GCAGC 6 cut(s) 50, 539, 658, 679, 920, 1079
BstX2I RGATCY 2 cut(s) 767, 1126
BstYI RGATCY 2 cut(s) 767, 1126
Bsu15I ATCGAT 1 cut(s) 1059
BsuRI GGCC 1 cut(s) 283
BsuTUI ATCGAT 1 cut(s) 1059
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 2 cut(s) 403, 635
BtsI GCAGTG 5 cut(s) 380, 572, 687, 783, 969
BtsIMutI CAGTG 8 cut(s) 380, 572, 687, 758, 783, 804, 894, 969
Cac8I GCNNGC 7 cut(s) 83, 128, 247, 438, 790, 794, 1102
CaiI CAGNNNCTG 1 cut(s) 692
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 1 cut(s) 282
ClaI ATCGAT 1 cut(s) 1059
CseI GACGC 2 cut(s) 411, 746
Csp6I GTAC 3 cut(s) 307, 533, 878
CviAII CATG 5 cut(s) 82, 279, 483, 865, 1028
CviQI GTAC 3 cut(s) 307, 533, 878
DdeI CTNAG 1 cut(s) 449
DpnI GATC 5 cut(s) 265, 769, 1058, 1062, 1128
DpnII GATC 5 cut(s) 263, 767, 1056, 1060, 1126
DraI TTTAAA 1 cut(s) 24
EciI GGCGGA 3 cut(s) 291, 342, 394
Eco24I GRGCYC 1 cut(s) 722
Eco31I GGTCTC 1 cut(s) 865
Eco57I CTGAAG 2 cut(s) 600, 1065
EcoNI CCTNNNNNAGG 1 cut(s) 706
EcoRII CCWGG 2 cut(s) 404, 937
EcoT38I GRGCYC 1 cut(s) 722
Esp3I CGTCTC 1 cut(s) 59
FaeI CATG 5 cut(s) 85, 282, 486, 868, 1031
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FatI CATG 5 cut(s) 81, 278, 482, 864, 1027
FauI CCCGC 1 cut(s) 68
FauNDI CATATG 1 cut(s) 988
Fnu4HI GCNGC 7 cut(s) 64, 325, 528, 672, 693, 909, 1093
FokI GGATG 2 cut(s) 410, 642
FriOI GRGCYC 1 cut(s) 722
Fsp4HI GCNGC 7 cut(s) 64, 325, 528, 672, 693, 909, 1093
FspBI CTAG 3 cut(s) 513, 797, 965
GluI GCNGC 7 cut(s) 64, 325, 528, 672, 693, 909, 1093
GsaI CCCAGC 2 cut(s) 274, 955
GsuI CTGGAG 2 cut(s) 828, 960
HaeIII GGCC 1 cut(s) 283
HapII CCGG 2 cut(s) 246, 774
HgaI GACGC 2 cut(s) 411, 746
Hin1I GRCGYC 1 cut(s) 422
Hin1II CATG 5 cut(s) 85, 282, 486, 868, 1031
HincII GTYRAC 1 cut(s) 429
HindII GTYRAC 1 cut(s) 429
HindIII AAGCTT 1 cut(s) 235
HinfI GANTC 4 cut(s) 15, 173, 368, 1048
HpaI GTTAAC 1 cut(s) 429
HpaII CCGG 2 cut(s) 246, 774
HphI GGTGA 2 cut(s) 168, 232
Hpy166II GTNNAC 3 cut(s) 126, 135, 429
Hpy188I TCNGA 6 cut(s) 172, 296, 490, 1056, 1072, 1084
Hpy188III TCNNGA 4 cut(s) 221, 827, 1036, 1124
Hpy8I GTNNAC 3 cut(s) 126, 135, 429
Hpy99I CGWCG 2 cut(s) 227, 427
HpyAV CCTTC 7 cut(s) 166, 172, 598, 619, 727, 1085, 1089
HpyCH4III ACNGT 5 cut(s) 50, 227, 464, 553, 838
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 9 cut(s) 71, 81, 126, 527, 680, 695, 908, 920, 1095
HpyF3I CTNAG 1 cut(s) 449
HpySE526I ACGT 1 cut(s) 291
Hsp92I GRCGYC 1 cut(s) 422
Hsp92II CATG 5 cut(s) 85, 282, 486, 868, 1031
KpnI GGTACC 1 cut(s) 881
KroI GCCGGC 1 cut(s) 245
KroNI GCCGGC 1 cut(s) 247
KspAI GTTAAC 1 cut(s) 429
Kzo9I GATC 5 cut(s) 263, 767, 1056, 1060, 1126
LmnI GCTCC 1 cut(s) 823
Lsp1109I GCAGC 6 cut(s) 50, 539, 658, 679, 920, 1079
LweI GCATC 1 cut(s) 810
MaeI CTAG 3 cut(s) 513, 797, 965
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 2 cut(s) 682, 1009
MalI GATC 5 cut(s) 265, 769, 1058, 1062, 1128
MboI GATC 5 cut(s) 263, 767, 1056, 1060, 1126
MboII GAAGA 4 cut(s) 144, 593, 762, 842
MflI RGATCY 2 cut(s) 767, 1126
MhlI GDGCHC 3 cut(s) 128, 405, 722
MluCI AATT 4 cut(s) 72, 474, 822, 944
MlyI GAGTC 4 cut(s) 24, 167, 377, 1057
MnlI CCTC 7 cut(s) 47, 202, 220, 323, 702, 1062, 1078
MroNI GCCGGC 1 cut(s) 245
MseI TTAA 4 cut(s) 23, 428, 1106, 1132
MspA1I CMGCKG 5 cut(s) 63, 324, 530, 911, 955
MspI CCGG 2 cut(s) 246, 774
MspR9I CCNGG 2 cut(s) 406, 939
Mva1269I GAATGC 1 cut(s) 920
MvaI CCWGG 2 cut(s) 406, 939
NaeI GCCGGC 1 cut(s) 247
NdeI CATATG 1 cut(s) 988
NdeII GATC 5 cut(s) 263, 767, 1056, 1060, 1126
NgoMIV GCCGGC 1 cut(s) 245
NlaIII CATG 5 cut(s) 85, 282, 486, 868, 1031
NlaIV GGNNCC 4 cut(s) 609, 664, 819, 879
NmeAIII GCCGAG 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 682
NspI RCATGY 2 cut(s) 85, 1031
NspV TTCGAA 1 cut(s) 95
PaeI GCATGC 1 cut(s) 85
PasI CCCWGGG 1 cut(s) 405
PctI GAATGC 1 cut(s) 920
PdiI GCCGGC 1 cut(s) 247
PflFI GACNNNGTC 1 cut(s) 484
PflMI CCANNNNNTGG 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 937
PkrI GCNGC 7 cut(s) 65, 326, 529, 673, 694, 910, 1094
Ple19I CGATCG 1 cut(s) 266
PleI GAGTC 4 cut(s) 23, 167, 376, 1056
PpsI GAGTC 4 cut(s) 23, 167, 376, 1056
PsiI TTATAA 1 cut(s) 459
Psp6I CCWGG 2 cut(s) 404, 937
PspFI CCCAGC 2 cut(s) 270, 951
PspGI CCWGG 2 cut(s) 404, 937
PspN4I GGNNCC 4 cut(s) 609, 664, 819, 879
PspPI GGNCC 1 cut(s) 282
PsrI GAACNNNNNNTAC 2 cut(s) 525, 557
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 1 cut(s) 692
PsuI RGATCY 2 cut(s) 767, 1126
PsyI GACNNNGTC 1 cut(s) 484
PvuI CGATCG 1 cut(s) 266
PvuII CAGCTG 3 cut(s) 530, 911, 955
RsaI GTAC 3 cut(s) 308, 534, 879
RsaNI GTAC 3 cut(s) 307, 533, 878
SaqAI TTAA 4 cut(s) 23, 428, 1106, 1132
SatI GCNGC 7 cut(s) 64, 325, 528, 672, 693, 909, 1093
Sau3AI GATC 5 cut(s) 263, 767, 1056, 1060, 1126
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 4 cut(s) 24, 167, 377, 1057
ScrFI CCNGG 2 cut(s) 406, 939
SduI GDGCHC 3 cut(s) 128, 405, 722
SfaNI GCATC 1 cut(s) 810
SfcI CTRYAG 3 cut(s) 141, 525, 620
SfuI TTCGAA 1 cut(s) 95
SphI GCATGC 1 cut(s) 85
Sse9I AATT 4 cut(s) 72, 474, 822, 944
SsiI CCGC 7 cut(s) 61, 243, 302, 324, 327, 379, 391
SspMI CTAG 3 cut(s) 513, 797, 965
StyD4I CCNGG 2 cut(s) 404, 937
TaaI ACNGT 5 cut(s) 50, 227, 464, 553, 838
TaiI ACGT 1 cut(s) 294
TaqI TCGA 6 cut(s) 18, 95, 222, 266, 479, 1059
TasI AATT 4 cut(s) 72, 474, 822, 944
TatI WGTACW 1 cut(s) 532
TauI GCSGC 1 cut(s) 327
Tru1I TTAA 4 cut(s) 23, 428, 1106, 1132
Tru9I TTAA 4 cut(s) 23, 428, 1106, 1132
TscAI CASTG 8 cut(s) 380, 579, 687, 765, 790, 811, 894, 976
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 6 cut(s) 63, 527, 671, 692, 908, 1092
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 1 cut(s) 267
TspRI CASTG 8 cut(s) 380, 579, 687, 765, 790, 811, 894, 976
Tth111I GACNNNGTC 1 cut(s) 484
Van91I CCANNNNNTGG 1 cut(s) 764
VneI GTGCAC 1 cut(s) 124
XagI CCTNNNNNAGG 1 cut(s) 706
XapI RAATTY 2 cut(s) 474, 944
XceI RCATGY 2 cut(s) 85, 1031
XspI CTAG 3 cut(s) 513, 797, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.