pycom13g09980

Ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002610
Physical Location & Seq
Reverse (-)
6667375 .. 6670304
2930 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2418 bp
ATGGACGAGAATGCCTTTTTCCCTCTCTCTCTCTCCACCTATATACACAATCACAAAAGCCAACCACGAAGCTCTCCAGACGTTTCGCTCTTCGGAGGTTCCGCAAAACGAAACGTTTCGGAAATGACGGTGTTCGGGAGCTCCGCCACGGCTTACCTGTCCGGCGGGAAAGCGCACGCGCACGTGTTTCCGATCGACTACGAGGCCGAGGTTTCGCAGCGGTTGGTGGACGCCGCGCACGACAACGACTCGAAATCGGCGGGGGAGTGTCTGGGCGATCCTTTCGTGGACGTGAACTTTGTCGGAACGGTGTGCTTGAAGTCGAAGAAGACGGAGATTGTGGCGCAGGGCGAGTCGGCGCACGAGGTTCGCGTCGAGTACGAGGAGTTCAAGACTCAGATCACTGCCTTATTCCTCGCCGCTCACTCTGGGAATTTGGCGCTCGTTAGGAAGCTCCTGAGTTATGGAGCAAATGTGAATCAAAAGCTGTTCCGAGGCTATGCAGCAACAGCAGCAGTAAGGGAGGGACATTTGGAGATACTGGAGGTGCTCATCAATGGCGGTGCGTCTCAGGAGGCATGTGAGGAGGCCTTGTTGGAGGCAAGCTACTTGGGGAGGGCTAGGTCCGCCGAGATGCTCATGGGATCCGACATGATCCGCCCTCAGGCGGCAGTGCACGCTCTTGTTTCTGCTTCCTGCAGAGGCTTTGTTGATGTAGTTGACACACTCATCAAGTGTGGGGTGGATGCGGATGCAACTGACAGGGCGTTGCTTCAATCGTCCAAGCCATTTCTGTACACTAATGTGTACTGTAATGCGCTTGTTGCCGCCATTGTTAGCCGGCAAATTTCTGTAGTCAGACTCCTCTTGCAGGCTGGAGCCAGAACAGATATAAAAGTGAGTCTTGGGGGCTGGTCCTGGGATGTAAATACAGGAGAAGAGTTTCGGGTGGGTGCAGGACTAGCCGAGGCTTACAGTGTGACCTGGTGTGCTGTGGAGTATTTTGAAGCCAGTGGTGCTATCTTGCGCATGCTCTTGCAACACCTCTCTCCTAACATTCCTCACTTTGGGAGGACTCTCATTCACCACGCCGTATTATGTAACAATGAAAGAGCGGTAGAGGTTCTGTTAAGTTGCGGTGCTGATGTAGAAGTTCCAATTAAGACAACAAATTCAGAAACTGACTGCCCTGTTCACATGGCGTCACGACTTGGATTAAATAAGGTTCTTCAACGATTGGTTAGTGCCGGGTGCAATATCAACTCTCGGACAGAATCTGGAGAAACTGCACTAATGACCTGTGCTAGATATAAACACCAGGAATGCCTTAAAATCCTGGCTTCAGACGGTGCTGATTTTGGCCTGGTGAATTCTGCTGGTCAGAGTGCAAGCGTAATAGCAGAGTCAGCAAGGTGGACTCTTGGCTTCCGACAAGCAGTTGCAGACGTGATTCGATTTGGTGAAATTGTTCAATCAAGCGATACATCAATATTTTCTCCTCTAATGCTTGTGACTCAAGCAAACGATGTTGAGGCCTTGAAGAAACTGATTGAGGGAGCAGACGTTGATCTGAATGAGCAAGATGAAAAGGGATACTCAGCTGCCATGATAGCTGCAGAGGGTGGTTACCTGGAAACTTTCAAATTGCTTATCGATGCTGGGGCTGACATAAACCTGCAGAATAAATGTGGTCAAACAGTGATGGAACTATTCGTTACAAACCAAAACAGTGAAGAATTTGAAAAGTTGTTTCTTAAGAATGCTGCACCTCAAAAGGTACTTGATAGTCCTATTGAGTTTTATGGGACTCTACATCAGGCGGTACAGCACGGCGACACAGATTTCGTTCTTACATTGATAAGCAGGGGTTCTGATGTGAATGCTTCTGGTGCCGATGGATACACTCCATTGATGTTAGCAGCAAGAGGAGGCCACGCTACGCTGTGCGAGCTTTTGATTACTTCAGGGGCAAAATGCGACATTGCGAATGCAAGACACGAGACAGCCCTCTTACTTGCAAGGAAAAGTGGGATTAAAAACGATGCAGAGAACGTGATATTGGATGAGCTTGCAAGAAGCCTTGTTCTTGGTGGGAGTCGTGTGAAGAAGCACACAAAATGCGGTAGAGGATCTCCACATCGAAAAATGCTGAAAATGGTTGGGGCTGCCGGGATTTTGCAGTGGGGGAAGTCGAGCAAGAGAAATGTGATTTGCAGGAGGGCGGAAGTCGGAGCTAGTGACTCGTTCAGATGGAACCGCCGGAGGAAATTCGACACTGACGAGCCAGGGCTGTTCCATGTGGTGACTACAAAGCACAAGGAGCTGCACTTTGTGTGTGAAGGTGGGATTGAAATGGCTGAGCTCTGGGTGAGAGGGATCAAGCTTGTGACAATCAAAGCTGTTTTTGGCAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4

Protein Analysis

806

Amino Acids

87.16

Weight (kDa)

6.2

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 132 - 189 1.7e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 137 - 215 7.9e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 390 - 454 1.5e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 501 - 585 5.9e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 519 - 570 5.1e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 604 - 680 1.4e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 604 - 653 1.2e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1028
Acc36I ACCTGC 1 cut(s) 1683
AccB1I GGYRCC 1 cut(s) 1889
AccBSI CCGCTC 2 cut(s) 422, 1115
AccII CGCG 3 cut(s) 179, 236, 372
AclI AACGTT 1 cut(s) 114
AclWI GGATC 6 cut(s) 272, 639, 649, 652, 2137, 2384
AcsI RAATTY 6 cut(s) 433, 846, 1171, 1369, 1736, 2267
AcuI CTGAAG 2 cut(s) 1326, 1947
AcvI CACGTG 1 cut(s) 184
AcyI GRCGYC 2 cut(s) 231, 1202
AdeI CACNNNGTG 1 cut(s) 2332
AfaI GTAC 5 cut(s) 380, 797, 809, 1779, 1824
AfiI CCNNNNNNNGG 4 cut(s) 664, 667, 871, 1067
AflII CTTAAG 1 cut(s) 1754
AflIII ACRYGT 1 cut(s) 183
AjiI CACGTC 2 cut(s) 292, 1447
AjnI CCWGG 7 cut(s) 917, 983, 1317, 1335, 1362, 1629, 2284
AjuI GAANNNNNNNTTGG 2 cut(s) 2042, 2074
AleI CACNNNNGTG 1 cut(s) 803
Alw21I GWGCWC 4 cut(s) 143, 552, 678, 2364
Alw26I GTCTC 2 cut(s) 573, 1994
Alw44I GTGCAC 1 cut(s) 674
AlwI GGATC 6 cut(s) 272, 639, 649, 652, 2137, 2384
AlwNI CAGNNNCTG 2 cut(s) 1181, 1277
AoxI GGCC 5 cut(s) 204, 588, 1360, 1533, 1930
ApaLI GTGCAC 1 cut(s) 674
ApeKI GCWGC 9 cut(s) 217, 503, 512, 1601, 1613, 1763, 1919, 2165, 2323
ApoI RAATTY 6 cut(s) 433, 846, 1171, 1369, 1736, 2267
ArsI GACNNNNNNTTYG 4 cut(s) 281, 313, 1826, 1858
Asp700I GAANNNNTTC 3 cut(s) 115, 942, 1467
AspLEI GCGC 8 cut(s) 175, 181, 238, 346, 361, 442, 820, 1029
AspS9I GGNCC 2 cut(s) 624, 915
AsuC2I CCSGG 2 cut(s) 1249, 2170
AsuHPI GGTGA 5 cut(s) 1076, 1378, 1472, 2314, 2380
AvaII GGWCC 2 cut(s) 624, 915
AxyI CCTNAGG 1 cut(s) 663
BaeGI GKGCMC 1 cut(s) 678
BamHI GGATCC 1 cut(s) 644
BanI GGYRCC 1 cut(s) 1889
BanII GRGCYC 2 cut(s) 143, 2364
BauI CACGAG 2 cut(s) 362, 1997
BbrPI CACGTG 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 335
Bbv12I GWGCWC 4 cut(s) 143, 552, 678, 2364
BbvI GCAGC 9 cut(s) 229, 515, 524, 1588, 1600, 1750, 1931, 2152, 2310
BccI CCATC 3 cut(s) 1696, 1889, 2244
BceAI ACGGC 3 cut(s) 165, 1076, 1846
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 7 cut(s) 919, 985, 1319, 1337, 1364, 1631, 2286
BciVI GTATCC 2 cut(s) 1586, 1892
BcnI CCSGG 2 cut(s) 1249, 2170
BcoDI GTCTC 2 cut(s) 573, 1994
BfaI CTAG 4 cut(s) 621, 962, 1305, 2235
BfmI CTRYAG 4 cut(s) 697, 852, 1614, 1676
BfoI RGCGCY 1 cut(s) 443
BfrI CTTAAG 1 cut(s) 1754
BfuAI ACCTGC 1 cut(s) 1683
BfuI GTATCC 2 cut(s) 1586, 1892
BlpI GCTNAGC 1 cut(s) 2358
Bme1390I CCNGG 9 cut(s) 919, 985, 1249, 1319, 1337, 1364, 1631, 2170, 2286
Bme18I GGWCC 2 cut(s) 624, 915
BmgBI CACGTC 2 cut(s) 292, 1447
BmgT120I GGNCC 2 cut(s) 624, 915
BmiI GGNNCC 5 cut(s) 100, 646, 880, 1891, 2255
BmrFI CCNGG 9 cut(s) 919, 985, 1249, 1319, 1337, 1364, 1631, 2170, 2286
BmsI GCATC 5 cut(s) 624, 736, 742, 1645, 2032
BpiI GAAGAC 1 cut(s) 335
BpmI CTGGAG 4 cut(s) 60, 563, 897, 1299
Bpu1102I GCTNAGC 1 cut(s) 2358
BpuEI CTTGAG 1 cut(s) 1500
BpuMI CCSGG 2 cut(s) 1249, 2170
Bsa29I ATCGAT 1 cut(s) 1653
BsaAI YACGTR 1 cut(s) 184
BsaHI GRCGYC 2 cut(s) 231, 1202
BsaJI CCNNGG 6 cut(s) 147, 207, 493, 918, 966, 2285
BsaXI ACNNNNNCTCC 6 cut(s) 459, 489, 578, 608, 1548, 1578
Bsc4I CCNNNNNNNGG 4 cut(s) 664, 667, 871, 1067
Bse118I RCCGGY 1 cut(s) 840
Bse1I ACTGG 2 cut(s) 546, 1011
Bse21I CCTNAGG 1 cut(s) 663
Bse3DI GCAATG 1 cut(s) 1980
BseBI CCWGG 7 cut(s) 919, 985, 1319, 1337, 1364, 1631, 2286
BseCI ATCGAT 1 cut(s) 1653
BseDI CCNNGG 6 cut(s) 147, 207, 493, 918, 966, 2285
BseGI GGATG 4 cut(s) 751, 757, 928, 2068
BseLI CCNNNNNNNGG 4 cut(s) 664, 667, 871, 1067
BseMI GCAATG 1 cut(s) 1980
BseMII CTCAG 6 cut(s) 410, 449, 584, 677, 1611, 2349
BseNI ACTGG 2 cut(s) 546, 1011
BseRI GAGGAG 5 cut(s) 398, 599, 854, 1488, 1941
BseSI GKGCMC 1 cut(s) 678
BseXI GCAGC 9 cut(s) 229, 515, 524, 1588, 1600, 1750, 1931, 2152, 2310
BseYI CCCAGC 1 cut(s) 1658
BsgI GTGCAG 4 cut(s) 975, 1272, 1749, 2309
Bsh1236I CGCG 3 cut(s) 179, 236, 372
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 5 cut(s) 206, 590, 1362, 1535, 1932
BshNI GGYRCC 1 cut(s) 1889
BshVI ATCGAT 1 cut(s) 1653
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 4 cut(s) 143, 552, 678, 2364
BsiSI CCGG 5 cut(s) 162, 841, 1248, 2169, 2260
BslFI GGGAC 2 cut(s) 540, 1819
BslI CCNNNNNNNGG 4 cut(s) 664, 667, 871, 1067
BsmAI GTCTC 2 cut(s) 573, 1994
BsmBI CGTCTC 1 cut(s) 573
BsmFI GGGAC 2 cut(s) 540, 1819
BsmI GAATGC 5 cut(s) 16, 1328, 1765, 1885, 1993
BsnI GGCC 5 cut(s) 206, 590, 1362, 1535, 1932
Bsp1286I GDGCHC 4 cut(s) 143, 552, 678, 2364
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 8 cut(s) 192, 277, 399, 644, 654, 1567, 2129, 2376
Bsp1720I GCTNAGC 1 cut(s) 2358
BspANI GGCC 5 cut(s) 206, 590, 1362, 1535, 1932
BspCNI CTCAG 6 cut(s) 409, 450, 583, 676, 1610, 2350
BspDI ATCGAT 1 cut(s) 1653
BspFNI CGCG 3 cut(s) 179, 236, 372
BspLI GGNNCC 5 cut(s) 100, 646, 880, 1891, 2255
BspMAI CTGCAG 3 cut(s) 701, 1618, 1680
BspMI ACCTGC 1 cut(s) 1683
BspPI GGATC 6 cut(s) 272, 639, 649, 652, 2137, 2384
BspQI GCTCTTC 1 cut(s) 95
BspT107I GGYRCC 1 cut(s) 1889
BspTI CTTAAG 1 cut(s) 1754
BsrBI CCGCTC 2 cut(s) 422, 1115
BsrDI GCAATG 1 cut(s) 1980
BsrFI RCCGGY 1 cut(s) 840
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 2 cut(s) 546, 1011
BssAI RCCGGY 1 cut(s) 840
BssECI CCNNGG 6 cut(s) 147, 207, 493, 918, 966, 2285
BssMI GATC 8 cut(s) 192, 277, 399, 644, 654, 1567, 2129, 2376
BssNI GRCGYC 2 cut(s) 231, 1202
BssSI CACGAG 2 cut(s) 362, 1997
Bst2BI CACGAG 2 cut(s) 362, 1997
Bst2UI CCWGG 7 cut(s) 919, 985, 1319, 1337, 1364, 1631, 2286
Bst4CI ACNGT 7 cut(s) 130, 310, 812, 977, 1349, 1699, 1730
Bst6I CTCTTC 2 cut(s) 95, 933
BstACI GRCGYC 2 cut(s) 231, 1202
BstAFI CTTAAG 1 cut(s) 1754
BstAUI TGTACA 1 cut(s) 795
BstBAI YACGTR 1 cut(s) 184
BstDEI CTNAG 6 cut(s) 396, 458, 570, 663, 1597, 2358
BstDSI CCRYGG 1 cut(s) 147
BstEII GGTNACC 1 cut(s) 1625
BstENI CCTNNNNNAGG 1 cut(s) 869
BstF5I GGATG 4 cut(s) 751, 757, 928, 2068
BstFNI CGCG 3 cut(s) 179, 236, 372
BstH2I RGCGCY 1 cut(s) 443
BstHHI GCGC 8 cut(s) 175, 181, 238, 346, 361, 442, 820, 1029
BstKTI GATC 8 cut(s) 195, 280, 402, 647, 657, 1570, 2132, 2379
BstMAI GTCTC 2 cut(s) 573, 1994
BstMBI GATC 8 cut(s) 192, 277, 399, 644, 654, 1567, 2129, 2376
BstMCI CGRYCG 1 cut(s) 195
BstNI CCWGG 7 cut(s) 919, 985, 1319, 1337, 1364, 1631, 2286
BstNSI RCATGY 2 cut(s) 582, 1033
BstPI GGTNACC 1 cut(s) 1625
BstSCI CCNGG 9 cut(s) 917, 983, 1247, 1317, 1335, 1362, 1629, 2168, 2284
BstSFI CTRYAG 4 cut(s) 697, 852, 1614, 1676
BstSLI GKGCMC 1 cut(s) 678
BstUI CGCG 3 cut(s) 179, 236, 372
BstV1I GCAGC 9 cut(s) 229, 515, 524, 1588, 1600, 1750, 1931, 2152, 2310
BstV2I GAAGAC 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 644, 2129
BstYI RGATCY 2 cut(s) 644, 2129
Bsu15I ATCGAT 1 cut(s) 1653
Bsu36I CCTNAGG 1 cut(s) 663
BsuI GTATCC 2 cut(s) 1586, 1892
BsuRI GGCC 5 cut(s) 206, 590, 1362, 1535, 1932
BsuTUI ATCGAT 1 cut(s) 1653
BtgI CCRYGG 1 cut(s) 147
BtrI CACGTC 2 cut(s) 292, 1447
BtsCI GGATG 4 cut(s) 751, 757, 928, 2068
BtsI GCAGTG 3 cut(s) 402, 678, 2186
BtsIMutI CAGTG 8 cut(s) 402, 678, 982, 1018, 1704, 1735, 2186, 2274
BveI ACCTGC 1 cut(s) 1683
CaiI CAGNNNCTG 2 cut(s) 1181, 1277
CfoI GCGC 8 cut(s) 175, 181, 238, 346, 361, 442, 820, 1029
Cfr10I RCCGGY 1 cut(s) 840
Cfr13I GGNCC 2 cut(s) 624, 915
ClaI ATCGAT 1 cut(s) 1653
CseI GACGC 4 cut(s) 239, 361, 555, 1191
CsiI ACCWGGT 1 cut(s) 983
Csp6I GTAC 5 cut(s) 379, 796, 808, 1778, 1823
CviAII CATG 7 cut(s) 579, 640, 652, 1030, 1198, 1606, 2297
CviQI GTAC 5 cut(s) 379, 796, 808, 1778, 1823
DdeI CTNAG 6 cut(s) 396, 458, 570, 663, 1597, 2358
DpnI GATC 8 cut(s) 194, 279, 401, 646, 656, 1569, 2131, 2378
DpnII GATC 8 cut(s) 192, 277, 399, 644, 654, 1567, 2129, 2376
DraIII CACNNNGTG 1 cut(s) 2332
Eam1104I CTCTTC 2 cut(s) 95, 933
EarI CTCTTC 2 cut(s) 95, 933
EciI GGCGGA 4 cut(s) 133, 616, 647, 2237
Ecl136II GAGCTC 2 cut(s) 141, 2362
Eco147I AGGCCT 2 cut(s) 590, 1535
Eco24I GRGCYC 2 cut(s) 143, 2364
Eco47I GGWCC 2 cut(s) 624, 915
Eco53kI GAGCTC 2 cut(s) 141, 2362
Eco57I CTGAAG 2 cut(s) 1326, 1947
Eco72I CACGTG 1 cut(s) 184
Eco81I CCTNAGG 1 cut(s) 663
Eco91I GGTNACC 1 cut(s) 1625
EcoICRI GAGCTC 2 cut(s) 141, 2362
EcoNI CCTNNNNNAGG 1 cut(s) 869
EcoO65I GGTNACC 1 cut(s) 1625
EcoRI GAATTC 1 cut(s) 1369
EcoRII CCWGG 7 cut(s) 917, 983, 1317, 1335, 1362, 1629, 2284
EcoT38I GRGCYC 2 cut(s) 143, 2364
Esp3I CGTCTC 1 cut(s) 573
FaeI CATG 7 cut(s) 582, 643, 655, 1033, 1201, 1609, 2300
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 2 cut(s) 540, 1819
FatI CATG 7 cut(s) 578, 639, 651, 1029, 1197, 1605, 2296
FauI CCCGC 2 cut(s) 158, 253
FokI GGATG 4 cut(s) 758, 764, 935, 2075
FriOI GRGCYC 2 cut(s) 143, 2364
FspBI CTAG 4 cut(s) 621, 962, 1305, 2235
FspI TGCGCA 1 cut(s) 1028
GlaI GCGC 8 cut(s) 174, 180, 237, 345, 360, 441, 819, 1028
GsaI CCCAGC 1 cut(s) 1662
GsuI CTGGAG 4 cut(s) 60, 563, 897, 1299
HaeII RGCGCY 1 cut(s) 443
HaeIII GGCC 5 cut(s) 206, 590, 1362, 1535, 1932
HapII CCGG 5 cut(s) 162, 841, 1248, 2169, 2260
HgaI GACGC 4 cut(s) 239, 361, 555, 1191
HhaI GCGC 8 cut(s) 175, 181, 238, 346, 361, 442, 820, 1029
Hin1I GRCGYC 2 cut(s) 231, 1202
Hin1II CATG 7 cut(s) 582, 643, 655, 1033, 1201, 1609, 2300
Hin6I GCGC 8 cut(s) 173, 179, 236, 344, 359, 440, 818, 1027
HinP1I GCGC 8 cut(s) 173, 179, 236, 344, 359, 440, 818, 1027
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HindIII AAGCTT 2 cut(s) 2381, 2411
HpaII CCGG 5 cut(s) 162, 841, 1248, 2169, 2260
HphI GGTGA 5 cut(s) 1076, 1378, 1472, 2314, 2380
Hpy166II GTNNAC 9 cut(s) 229, 289, 295, 676, 721, 798, 808, 1195, 1416
Hpy188III TCNNGA 7 cut(s) 77, 136, 391, 457, 572, 1206, 1278
Hpy8I GTNNAC 9 cut(s) 229, 289, 295, 676, 721, 798, 808, 1195, 1416
Hpy99I CGWCG 1 cut(s) 377
HpyAV CCTTC 1 cut(s) 2333
HpyCH4III ACNGT 7 cut(s) 130, 310, 812, 977, 1349, 1699, 1730
HpyCH4IV ACGT 7 cut(s) 81, 114, 183, 291, 1446, 1563, 2052
HpyF3I CTNAG 6 cut(s) 396, 458, 570, 663, 1597, 2358
HpySE526I ACGT 7 cut(s) 81, 114, 183, 291, 1446, 1563, 2052
Hsp92I GRCGYC 2 cut(s) 231, 1202
Hsp92II CATG 7 cut(s) 582, 643, 655, 1033, 1201, 1609, 2300
HspAI GCGC 8 cut(s) 173, 179, 236, 344, 359, 440, 818, 1027
KroI GCCGGC 1 cut(s) 840
KroNI GCCGGC 1 cut(s) 842
Kzo9I GATC 8 cut(s) 192, 277, 399, 644, 654, 1567, 2129, 2376
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 8 cut(s) 138, 146, 459, 467, 878, 1556, 2231, 2320
Lsp1109I GCAGC 9 cut(s) 229, 515, 524, 1588, 1600, 1750, 1931, 2152, 2310
LweI GCATC 5 cut(s) 624, 736, 742, 1645, 2032
MabI ACCWGGT 1 cut(s) 983
MaeI CTAG 4 cut(s) 621, 962, 1305, 2235
MaeII ACGT 7 cut(s) 81, 114, 183, 291, 1446, 1563, 2052
MaeIII GTNAC 9 cut(s) 979, 1100, 1203, 1510, 1625, 1714, 2237, 2302, 2386
MalI GATC 8 cut(s) 194, 279, 401, 646, 656, 1569, 2131, 2378
MbiI CCGCTC 2 cut(s) 422, 1115
MboI GATC 8 cut(s) 192, 277, 399, 644, 654, 1567, 2129, 2376
MboII GAAGA 8 cut(s) 82, 337, 340, 950, 1220, 1552, 1745, 2116
MflI RGATCY 2 cut(s) 644, 2129
MhlI GDGCHC 4 cut(s) 143, 552, 678, 2364
MluCI AATT 9 cut(s) 433, 846, 1158, 1171, 1369, 1464, 1643, 1736, 2267
MmeI TCCRAC 5 cut(s) 283, 576, 672, 1453, 2209
MroNI GCCGGC 1 cut(s) 840
MroXI GAANNNNTTC 3 cut(s) 115, 942, 1467
MseI TTAA 6 cut(s) 1130, 1161, 1217, 1329, 1755, 2034
MslI CAYNNNNRTG 1 cut(s) 803
MspA1I CMGCKG 2 cut(s) 220, 1601
MspCI CTTAAG 1 cut(s) 1754
MspI CCGG 5 cut(s) 162, 841, 1248, 2169, 2260
MspR9I CCNGG 9 cut(s) 919, 985, 1249, 1319, 1337, 1364, 1631, 2170, 2286
Mva1269I GAATGC 5 cut(s) 16, 1328, 1765, 1885, 1993
MvaI CCWGG 7 cut(s) 919, 985, 1319, 1337, 1364, 1631, 2286
MvnI CGCG 3 cut(s) 179, 236, 372
NaeI GCCGGC 1 cut(s) 842
NciI CCSGG 2 cut(s) 1249, 2170
NdeII GATC 8 cut(s) 192, 277, 399, 644, 654, 1567, 2129, 2376
NgoMIV GCCGGC 1 cut(s) 840
NlaIII CATG 7 cut(s) 582, 643, 655, 1033, 1201, 1609, 2300
NlaIV GGNNCC 5 cut(s) 100, 646, 880, 1891, 2255
NmeAIII GCCGAG 3 cut(s) 232, 655, 991
NmuCI GTSAC 6 cut(s) 979, 1203, 1510, 2237, 2302, 2386
NsbI TGCGCA 1 cut(s) 1028
NspI RCATGY 2 cut(s) 582, 1033
OliI CACNNNNGTG 1 cut(s) 803
PaeI GCATGC 1 cut(s) 1033
PceI AGGCCT 2 cut(s) 590, 1535
PciSI GCTCTTC 1 cut(s) 95
PcsI WCGNNNNNNNCGW 3 cut(s) 237, 369, 2277
PctI GAATGC 5 cut(s) 16, 1328, 1765, 1885, 1993
PdiI GCCGGC 1 cut(s) 842
PdmI GAANNNNTTC 3 cut(s) 115, 942, 1467
PfeI GAWTC 3 cut(s) 478, 1274, 1450
Ple19I CGATCG 1 cut(s) 195
PmaCI CACGTG 1 cut(s) 184
PmlI CACGTG 1 cut(s) 184
Ppu21I YACGTR 1 cut(s) 184
Psp124BI GAGCTC 2 cut(s) 143, 2364
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 7 cut(s) 917, 983, 1317, 1335, 1362, 1629, 2284
PspCI CACGTG 1 cut(s) 184
PspEI GGTNACC 1 cut(s) 1625
PspFI CCCAGC 1 cut(s) 1658
PspGI CCWGG 7 cut(s) 917, 983, 1317, 1335, 1362, 1629, 2284
PspN4I GGNNCC 5 cut(s) 100, 646, 880, 1891, 2255
PspPI GGNCC 2 cut(s) 624, 915
PsrI GAACNNNNNNTAC 2 cut(s) 371, 403
PstI CTGCAG 3 cut(s) 701, 1618, 1680
PstNI CAGNNNCTG 2 cut(s) 1181, 1277
PsuI RGATCY 2 cut(s) 644, 2129
PvuI CGATCG 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 1601
RsaI GTAC 5 cut(s) 380, 797, 809, 1779, 1824
RsaNI GTAC 5 cut(s) 379, 796, 808, 1778, 1823
RseI CAYNNNNRTG 1 cut(s) 803
SacI GAGCTC 2 cut(s) 143, 2364
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 6 cut(s) 1130, 1161, 1217, 1329, 1755, 2034
Sau3AI GATC 8 cut(s) 192, 277, 399, 644, 654, 1567, 2129, 2376
Sau96I GGNCC 2 cut(s) 624, 915
ScrFI CCNGG 9 cut(s) 919, 985, 1249, 1319, 1337, 1364, 1631, 2170, 2286
SduI GDGCHC 4 cut(s) 143, 552, 678, 2364
SexAI ACCWGGT 1 cut(s) 983
SfaNI GCATC 5 cut(s) 624, 736, 742, 1645, 2032
SfcI CTRYAG 4 cut(s) 697, 852, 1614, 1676
SinI GGWCC 2 cut(s) 624, 915
SmiMI CAYNNNNRTG 1 cut(s) 803
SmlI CTYRAG 2 cut(s) 1515, 1754
SmoI CTYRAG 2 cut(s) 1515, 1754
SphI GCATGC 1 cut(s) 1033
Sse9I AATT 9 cut(s) 433, 846, 1158, 1171, 1369, 1464, 1643, 1736, 2267
SseBI AGGCCT 2 cut(s) 590, 1535
SspI AATATT 1 cut(s) 1491
SspMI CTAG 4 cut(s) 621, 962, 1305, 2235
SstI GAGCTC 2 cut(s) 143, 2364
StuI AGGCCT 2 cut(s) 590, 1535
StyD4I CCNGG 9 cut(s) 917, 983, 1247, 1317, 1335, 1362, 1629, 2168, 2284
TaaI ACNGT 7 cut(s) 130, 310, 812, 977, 1349, 1699, 1730
TaiI ACGT 7 cut(s) 84, 117, 186, 294, 1449, 1566, 2055
TaqI TCGA 9 cut(s) 195, 251, 323, 375, 1453, 1653, 2140, 2192, 2271
TasI AATT 9 cut(s) 433, 846, 1158, 1171, 1369, 1464, 1643, 1736, 2267
TatI WGTACW 2 cut(s) 795, 807
TauI GCSGC 4 cut(s) 236, 422, 671, 830
TfiI GAWTC 3 cut(s) 478, 1274, 1450
Tru1I TTAA 6 cut(s) 1130, 1161, 1217, 1329, 1755, 2034
Tru9I TTAA 6 cut(s) 1130, 1161, 1217, 1329, 1755, 2034
TscAI CASTG 8 cut(s) 409, 678, 982, 1018, 1704, 1735, 2186, 2281
TseFI GTSAC 6 cut(s) 979, 1203, 1510, 2237, 2302, 2386
TseI GCWGC 9 cut(s) 217, 503, 512, 1601, 1613, 1763, 1919, 2165, 2323
Tsp45I GTSAC 6 cut(s) 979, 1203, 1510, 2237, 2302, 2386
TspDTI ATGAA 2 cut(s) 1122, 1599
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 8 cut(s) 409, 678, 982, 1018, 1704, 1735, 2186, 2281
Vha464I CTTAAG 1 cut(s) 1754
VneI GTGCAC 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 624, 915
XagI CCTNNNNNAGG 1 cut(s) 869
XapI RAATTY 6 cut(s) 433, 846, 1171, 1369, 1736, 2267
XceI RCATGY 2 cut(s) 582, 1033
XmnI GAANNNNTTC 3 cut(s) 115, 942, 1467
XspI CTAG 4 cut(s) 621, 962, 1305, 2235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.