pycom13g16620

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003569
Physical Location & Seq
Reverse (-)
12397556 .. 12398303
748 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 699 bp
ATGTCTAAAATCCAATGCAAGAACTTCCACTGCTTCCCTAATTTCATCAAATGCCTGCCCACAATCCAAGCTCCACCGGATCAAGCCTCAAACGAATTAGACCACCAAAACCAGCCACCACGTCCCTCTACCACCACATGTACATCTCTTATCATCAAGAACTTCAACTCCCTCTACCATGACTTCAACTTGGTGAACTCATCATCAGATCACTCAACCTTCAAATCCCTCACAGCCTCCTCCTCCTCCGCCGACGATCACTTCTACAACTCCTCCGAAGAATCAGACGCCGACCAGTATTCCCCTCCAGATTTCGCCACCATCTTCGCCTCTCAACGCTTCTTCTTCTCCTCCCCGGGACAATCCAACTCCATCGTCGAGTCTCCAGACACCACCACAAAACCCAAGTCTTCTCACAAAAGAATGTTGGTCACCAAAGGCGTGCAAGTTCCCAAGTATTCCTCAAACCCTTACATGGATTTTCGGCGTGATCATGTTCATGCTACAAGCGATGACATGGAGTATTTACATGAGCTTCTCTTGTGCTACCTTTCGCTCAATCCGACACACGCGCACAAGGACATTATCAGTGCCTTCACCGATCTTGTCATCGGCCTACTGTGTGCTGAAGATTCACCAGCACCTGCACCAGCTTCAGCGGCTGATATGACCGTGAAAAAAAAGCCGGAAACTCATTAG

Protein Analysis

233

Amino Acids

25.82

Weight (kDa)

5.9

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 154 - 206 6.3e-13 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 652
Acc36I ACCTGC 1 cut(s) 652
AccII CGCG 1 cut(s) 572
AciI CCGC 2 cut(s) 249, 659
AclWI GGATC 1 cut(s) 87
AcuI CTGAAG 2 cut(s) 639, 648
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 1 cut(s) 142
AfiI CCNNNNNNNGG 1 cut(s) 475
AflIII ACRYGT 1 cut(s) 137
AgsI TTSAA 3 cut(s) 166, 187, 223
AjiI CACGTC 1 cut(s) 122
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 3 cut(s) 71, 535, 653
AluI AGCT 3 cut(s) 71, 535, 653
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 1 cut(s) 87
AlwNI CAGNNNCTG 2 cut(s) 644, 662
Ama87I CYCGRG 1 cut(s) 355
AoxI GGCC 1 cut(s) 613
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AspLEI GCGC 1 cut(s) 574
AsuC2I CCSGG 2 cut(s) 356, 357
AsuHPI GGTGA 4 cut(s) 205, 424, 589, 627
AvaI CYCGRG 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 402
BccI CCATC 2 cut(s) 329, 380
BclI TGATCA 1 cut(s) 490
BcnI CCSGG 2 cut(s) 356, 357
BcoDI GTCTC 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 652
BisI GCNGC 1 cut(s) 660
BlsI GCNGC 1 cut(s) 661
Bme1390I CCNGG 2 cut(s) 356, 357
BmeT110I CYCGRG 1 cut(s) 355
BmgBI CACGTC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 356, 357
BpiI GAAGAC 1 cut(s) 402
BpmI CTGGAG 2 cut(s) 291, 369
BpuMI CCSGG 2 cut(s) 356, 357
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 354, 355
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse1I ACTGG 1 cut(s) 295
BseDI CCNNGG 2 cut(s) 354, 355
BseLI CCNNNNNNNGG 1 cut(s) 475
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 5 cut(s) 229, 232, 235, 262, 340
BsgI GTGCAG 1 cut(s) 630
Bsh1236I CGCG 1 cut(s) 572
BshFI GGCC 1 cut(s) 615
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 3 cut(s) 77, 356, 686
BslFI GGGAC 2 cut(s) 108, 372
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 387
BsmFI GGGAC 2 cut(s) 108, 372
BsnI GGCC 1 cut(s) 615
BsoBI CYCGRG 1 cut(s) 355
Bsp1407I TGTACA 1 cut(s) 140
Bsp143I GATC 5 cut(s) 79, 208, 256, 490, 601
BspACI CCGC 2 cut(s) 249, 659
BspANI GGCC 1 cut(s) 615
BspFNI CGCG 1 cut(s) 572
BspMI ACCTGC 1 cut(s) 652
BspPI GGATC 1 cut(s) 87
BsrGI TGTACA 1 cut(s) 140
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 2 cut(s) 354, 355
BssMI GATC 5 cut(s) 79, 208, 256, 490, 601
BssNI GRCGYC 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 621, 673
BstACI GRCGYC 1 cut(s) 288
BstAUI TGTACA 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 56, 443
BstEII GGTNACC 1 cut(s) 430
BstFNI CGCG 1 cut(s) 572
BstHHI GCGC 1 cut(s) 574
BstKTI GATC 5 cut(s) 82, 211, 259, 493, 604
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 5 cut(s) 79, 208, 256, 490, 601
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNSI RCATGY 1 cut(s) 141
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 2 cut(s) 354, 355
BstUI CGCG 1 cut(s) 572
BstV2I GAAGAC 1 cut(s) 402
BsuRI GGCC 1 cut(s) 615
BtgZI GCGATG 1 cut(s) 525
BtrI CACGTC 1 cut(s) 122
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 2 cut(s) 28, 595
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 2 cut(s) 56, 443
CaiI CAGNNNCTG 2 cut(s) 644, 662
CfoI GCGC 1 cut(s) 574
Cfr9I CCCGGG 1 cut(s) 355
CseI GACGC 1 cut(s) 296
Csp6I GTAC 1 cut(s) 141
CviAII CATG 7 cut(s) 138, 179, 475, 494, 500, 517, 530
CviJI RGCY 9 cut(s) 71, 86, 115, 236, 535, 615, 653, 662, 685
CviKI_1 RGCY 9 cut(s) 71, 86, 115, 236, 535, 615, 653, 662, 685
CviQI GTAC 1 cut(s) 141
DpnI GATC 5 cut(s) 81, 210, 258, 492, 603
DpnII GATC 5 cut(s) 79, 208, 256, 490, 601
EciI GGCGGA 1 cut(s) 238
Eco57I CTGAAG 2 cut(s) 639, 648
Eco88I CYCGRG 1 cut(s) 355
Eco91I GGTNACC 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 430
FaeI CATG 7 cut(s) 141, 182, 478, 497, 503, 520, 533
FaiI YATR 8 cut(s) 139, 180, 476, 495, 501, 518, 531, 668
FaqI GGGAC 2 cut(s) 108, 372
FatI CATG 7 cut(s) 137, 178, 474, 493, 499, 516, 529
FbaI TGATCA 1 cut(s) 490
Fnu4HI GCNGC 1 cut(s) 660
Fsp4HI GCNGC 1 cut(s) 660
GlaI GCGC 1 cut(s) 573
GluI GCNGC 1 cut(s) 660
GsuI CTGGAG 2 cut(s) 291, 369
HaeIII GGCC 1 cut(s) 615
HapII CCGG 3 cut(s) 77, 356, 686
HgaI GACGC 1 cut(s) 296
HhaI GCGC 1 cut(s) 574
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 7 cut(s) 141, 182, 478, 497, 503, 520, 533
Hin6I GCGC 1 cut(s) 572
HinP1I GCGC 1 cut(s) 572
HinfI GANTC 3 cut(s) 281, 380, 632
HpaII CCGG 3 cut(s) 77, 356, 686
HphI GGTGA 4 cut(s) 205, 424, 589, 627
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 4 cut(s) 208, 277, 286, 564
Hpy188III TCNNGA 3 cut(s) 157, 308, 386
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 2 cut(s) 257, 380
HpyAV CCTTC 2 cut(s) 229, 604
HpyCH4III ACNGT 2 cut(s) 621, 673
HpyCH4IV ACGT 1 cut(s) 121
HpyCH4V TGCA 3 cut(s) 18, 445, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpySE526I ACGT 1 cut(s) 121
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 7 cut(s) 141, 182, 478, 497, 503, 520, 533
HspAI GCGC 1 cut(s) 572
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 5 cut(s) 79, 208, 256, 490, 601
LmnI GCTCC 1 cut(s) 76
MaeII ACGT 1 cut(s) 121
MaeIII GTNAC 1 cut(s) 430
MalI GATC 5 cut(s) 81, 210, 258, 492, 603
MboI GATC 5 cut(s) 79, 208, 256, 490, 601
MboII GAAGA 6 cut(s) 290, 316, 334, 337, 402, 641
MluCI AATT 2 cut(s) 40, 95
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 2 cut(s) 390, 587
MslI CAYNNNNRTG 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 3 cut(s) 77, 356, 686
MspR9I CCNGG 2 cut(s) 356, 357
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 1 cut(s) 659
NciI CCSGG 2 cut(s) 356, 357
NdeII GATC 5 cut(s) 79, 208, 256, 490, 601
NlaIII CATG 7 cut(s) 141, 182, 478, 497, 503, 520, 533
NmuCI GTSAC 1 cut(s) 430
NspI RCATGY 1 cut(s) 141
PaqCI CACCTGC 1 cut(s) 652
PciI ACATGT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 560
PfeI GAWTC 2 cut(s) 281, 632
PkrI GCNGC 1 cut(s) 661
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PscI ACATGT 1 cut(s) 137
PspEI GGTNACC 1 cut(s) 430
PstNI CAGNNNCTG 2 cut(s) 644, 662
RsaI GTAC 1 cut(s) 142
RsaNI GTAC 1 cut(s) 141
RseI CAYNNNNRTG 1 cut(s) 498
SatI GCNGC 1 cut(s) 660
Sau3AI GATC 5 cut(s) 79, 208, 256, 490, 601
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 356, 357
SetI ASST 7 cut(s) 73, 124, 221, 537, 552, 646, 655
SmaI CCCGGG 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 498
Sse9I AATT 2 cut(s) 40, 95
SsiI CCGC 2 cut(s) 249, 659
StyD4I CCNGG 2 cut(s) 354, 355
TaaI ACNGT 2 cut(s) 621, 673
TaiI ACGT 1 cut(s) 124
TaqI TCGA 1 cut(s) 378
TasI AATT 2 cut(s) 40, 95
TatI WGTACW 1 cut(s) 140
TauI GCSGC 1 cut(s) 662
TfiI GAWTC 2 cut(s) 281, 632
TscAI CASTG 2 cut(s) 35, 595
TseFI GTSAC 1 cut(s) 430
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 2 cut(s) 34, 488
TspMI CCCGGG 1 cut(s) 355
TspRI CASTG 2 cut(s) 35, 595
XceI RCATGY 1 cut(s) 141
XmaI CCCGGG 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.