pycom14g04740

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001646
Physical Location & Seq
Forward (+)
4468162 .. 4470244
2083 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
ATGGCCACGACTGCAGCTCCCGGATCACACATGGCAGCGGTTAAGCGTTGCATATCGTCCTCTCAAAGAACTTATATGTCATGCACAGCAAAGTTCGGTACAAACAGCGAGGTGGGAGTTTGGCGAAGGCTTAAGAGTTCTTCGCACATCTCAGCAACACAGCCATTCTCCCAGAGCTTGAAATCAGGGCCTGCGAAATCCGTGAAGGTTGTCACAAGAGCAATGTCAGGAGTAGCTGAGAACACTCCACTACCAGGATTGCCTATTGATTTGAGAGGAAAGAGAGCATTCATTGCTGGTGTGGCTGATGATCATGGATATGGATGGGCAATATCAAAAGCTCTTGCTGCTGCTGGGGCTGAAATTCTAGTTGGTACATGGGTGCCGGCACTGAACATTTTTGAGTCCAGTTTAAGGCGTGGAAAGTTTGACGAGACACGCAAACTGCCAGATGGCTCTTTGATGGAAATTACCAAGGTTTACCCATTAGATGCAGTTTTTGACAGTCCCGAGGATGTTCCTGAAGATATAAAAACCAACAAACGCTACTTGGGGTCCTCAAATTGGACTGTTAAGGAGGTTGTGGAATCTGTGAAGCAGGATTTTGGCAGCATTGATATCCTCGTCCACTCTCTTGCCAATGGGCCAGAGGTTACCAAACCTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

223

Amino Acids

23.84

Weight (kDa)

9.2

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AciI CCGC 1 cut(s) 38
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 2 cut(s) 100, 376
AfiI CCNNNNNNNGG 3 cut(s) 254, 414, 564
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 1 cut(s) 253
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AluBI AGCT 4 cut(s) 17, 177, 236, 341
AluI AGCT 4 cut(s) 17, 177, 236, 341
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 1 cut(s) 31
AlwNI CAGNNNCTG 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 3 cut(s) 3, 188, 644
ApeKI GCWGC 5 cut(s) 14, 35, 347, 350, 609
ApoI RAATTY 1 cut(s) 363
AspS9I GGNCC 3 cut(s) 188, 555, 644
AsuC2I CCSGG 1 cut(s) 21
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 1 cut(s) 555
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 382
BbvI GCAGC 5 cut(s) 26, 47, 334, 337, 621
BccI CCATC 3 cut(s) 318, 446, 457
BciT130I CCWGG 1 cut(s) 255
BclI TGATCA 1 cut(s) 310
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 12
BfrI CTTAAG 1 cut(s) 131
BisI GCNGC 5 cut(s) 15, 36, 348, 351, 610
BlsI GCNGC 5 cut(s) 16, 37, 349, 352, 611
Bme1390I CCNGG 2 cut(s) 21, 255
Bme18I GGWCC 1 cut(s) 555
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 3 cut(s) 188, 555, 644
BmiI GGNNCC 2 cut(s) 384, 556
BmrFI CCNGG 2 cut(s) 21, 255
BmsI GCATC 1 cut(s) 481
BpuMI CCSGG 1 cut(s) 21
BsaJI CCNNGG 2 cut(s) 474, 510
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 414, 564
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 2 cut(s) 228, 291
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 2 cut(s) 474, 510
BseGI GGATG 2 cut(s) 329, 520
BseLI CCNNNNNNNGG 3 cut(s) 254, 414, 564
BseMI GCAATG 2 cut(s) 228, 291
BseMII CTCAG 2 cut(s) 165, 228
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 5 cut(s) 26, 47, 334, 337, 621
BseYI CCCAGC 1 cut(s) 353
BshFI GGCC 3 cut(s) 5, 190, 646
BshNI GGYRCC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 2 cut(s) 21, 386
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 3 cut(s) 254, 414, 564
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 3 cut(s) 5, 190, 646
BsoBI CYCGRG 1 cut(s) 509
Bsp143I GATC 2 cut(s) 23, 310
BspACI CCGC 1 cut(s) 38
BspANI GGCC 3 cut(s) 5, 190, 646
BspCNI CTCAG 2 cut(s) 164, 229
BspLI GGNNCC 2 cut(s) 384, 556
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 1 cut(s) 31
BspT107I GGYRCC 1 cut(s) 382
BspTI CTTAAG 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 228, 291
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 2 cut(s) 474, 510
BssMI GATC 2 cut(s) 23, 310
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 2 cut(s) 506, 571
BstAFI CTTAAG 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 2 cut(s) 192, 387
BstDEI CTNAG 2 cut(s) 151, 237
BstEII GGTNACC 1 cut(s) 652
BstF5I GGATG 2 cut(s) 329, 520
BstKTI GATC 2 cut(s) 26, 313
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 2 cut(s) 23, 310
BstMWI GCNNNNNNNGC 5 cut(s) 11, 293, 302, 347, 356
BstNI CCWGG 1 cut(s) 255
BstPI GGTNACC 1 cut(s) 652
BstSCI CCNGG 2 cut(s) 19, 253
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 5 cut(s) 26, 47, 334, 337, 621
BsuRI GGCC 3 cut(s) 5, 190, 646
BtsCI GGATG 2 cut(s) 329, 520
BtsIMutI CAGTG 1 cut(s) 389
Cac8I GCNNGC 2 cut(s) 192, 387
CaiI CAGNNNCTG 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 3 cut(s) 188, 555, 644
Csp6I GTAC 2 cut(s) 99, 375
CviAII CATG 4 cut(s) 31, 81, 314, 378
CviQI GTAC 2 cut(s) 99, 375
DdeI CTNAG 2 cut(s) 151, 237
DpnI GATC 2 cut(s) 25, 312
DpnII GATC 2 cut(s) 23, 310
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 474
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 1 cut(s) 555
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 652
EcoO109I RGGNCCY 2 cut(s) 188, 555
EcoO65I GGTNACC 1 cut(s) 652
EcoRII CCWGG 1 cut(s) 253
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 4 cut(s) 34, 84, 317, 381
FaiI YATR 9 cut(s) 32, 53, 75, 77, 82, 315, 321, 379, 530
FaqI GGGAC 1 cut(s) 492
FatI CATG 4 cut(s) 30, 80, 313, 377
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 5 cut(s) 15, 36, 348, 351, 610
FokI GGATG 2 cut(s) 336, 527
Fsp4HI GCNGC 5 cut(s) 15, 36, 348, 351, 610
FspBI CTAG 1 cut(s) 368
GluI GCNGC 5 cut(s) 15, 36, 348, 351, 610
GsaI CCCAGC 1 cut(s) 357
HaeIII GGCC 3 cut(s) 5, 190, 646
HapII CCGG 2 cut(s) 21, 386
Hin1II CATG 4 cut(s) 34, 84, 317, 381
HinfI GANTC 2 cut(s) 404, 587
HpaII CCGG 2 cut(s) 21, 386
Hpy166II GTNNAC 2 cut(s) 481, 628
Hpy188III TCNNGA 3 cut(s) 228, 509, 521
Hpy8I GTNNAC 2 cut(s) 481, 628
HpyAV CCTTC 2 cut(s) 120, 199
HpyCH4III ACNGT 2 cut(s) 506, 571
HpyCH4V TGCA 4 cut(s) 14, 51, 84, 494
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 293, 302, 347, 356
HpyF3I CTNAG 2 cut(s) 151, 237
Hsp92II CATG 4 cut(s) 34, 84, 317, 381
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 2 cut(s) 23, 310
LmnI GCTCC 1 cut(s) 22
Lsp1109I GCAGC 5 cut(s) 26, 47, 334, 337, 621
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 368
MaeIII GTNAC 2 cut(s) 211, 652
MalI GATC 2 cut(s) 25, 312
MboI GATC 2 cut(s) 23, 310
MboII GAAGA 2 cut(s) 132, 536
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 363, 468, 562
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 413
MnlI CCTC 8 cut(s) 70, 103, 269, 505, 568, 571, 632, 643
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 385
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 42, 132, 413, 573
MslI CAYNNNNRTG 1 cut(s) 318
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 38
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 2 cut(s) 21, 386
MspR9I CCNGG 2 cut(s) 21, 255
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 11, 293, 302, 347, 356
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 1 cut(s) 21
NdeII GATC 2 cut(s) 23, 310
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 4 cut(s) 34, 84, 317, 381
NlaIV GGNNCC 2 cut(s) 384, 556
NmuCI GTSAC 1 cut(s) 211
PctI GAATGC 1 cut(s) 287
PdiI GCCGGC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 587
PfoI TCCNGGA 1 cut(s) 19
PkrI GCNGC 5 cut(s) 16, 37, 349, 352, 611
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
PpuMI RGGWCCY 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 1 cut(s) 253
PspEI GGTNACC 1 cut(s) 652
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 384, 556
PspPI GGNCC 3 cut(s) 188, 555, 644
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 1 cut(s) 16
PstNI CAGNNNCTG 1 cut(s) 191
RsaI GTAC 2 cut(s) 100, 376
RsaNI GTAC 2 cut(s) 99, 375
RseI CAYNNNNRTG 1 cut(s) 318
SaqAI TTAA 4 cut(s) 42, 132, 413, 573
SatI GCNGC 5 cut(s) 15, 36, 348, 351, 610
Sau3AI GATC 2 cut(s) 23, 310
Sau96I GGNCC 3 cut(s) 188, 555, 644
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 2 cut(s) 21, 255
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 1 cut(s) 555
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 3 cut(s) 363, 468, 562
SsiI CCGC 1 cut(s) 38
SspMI CTAG 1 cut(s) 368
StyD4I CCNGG 2 cut(s) 19, 253
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 2 cut(s) 506, 571
TasI AATT 3 cut(s) 363, 468, 562
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 4 cut(s) 42, 132, 413, 573
Tru9I TTAA 4 cut(s) 42, 132, 413, 573
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 5 cut(s) 14, 35, 347, 350, 609
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 1 cut(s) 280
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 396
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 555
XapI RAATTY 1 cut(s) 363
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.