pycom14g11300

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004963
Physical Location & Seq
Reverse (-)
14266199 .. 14267185
987 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g11300.1

Sequence Viewer

Length: 987 bp
ATGGGTTGTGTTTCTTCCAATCTCTTCAACCACGACGACGAGTTCTCCCAACTCGGCAGCTCCGCGCTCAGCCACCACATCGTCTCGCTCACTTCCTCCACCTACGGCCTTCTTACTCTAGACCCGCCGCCGCCTGCTCCCACCACTCCGCCGACCCCTCCTCCTCGATTCACTCTCGGGTCCATTTTTCCGAGCCCCCTGTCGGAGCCCAAGTCGCTCTGGTCTGAACCCAGGTCGCTATGGTCCGACCCGAGGCCGCTACGGTCCGACCCGGAAGTCATTAACTCATGGGAGCTTATGGCCGGCCTCGACGCTGATAGTTTCCGATTCTCGCCGCTCCCGCCGCCCAAACCCTTCGTTTTCAATGACCCAAAAACCCCCGATAAAGAAAACTCCAACCCGAATCGGGCAATTTTCAAATCTCCGTACGACGGTGTCTTCCGGGTCAACAACAAGAAGGACCCACTTGACCGGTTCGAAAAAATATGCCCACCGGGAGGAGAAAGCAAAGTGGTGGTCTACACTACGACGCTGCGAGGAGTGCGGCGGACTTTCGAGGAGTGTAACGCCGTCCGGGCGGCGATAGAAGGCCTCGGAGTGATGATTGTCGAGCGTGACGTGTCGATGGATCGCGGGTTCAGAGAAGAGTTGAACGATTTGATGAAGGAGAAAGGGAAGGATGTGGCGGTGCCGCCGCAGGTTTTTGTGAAGGGGAGATATATAGGCGGCGCTGAGGAGGTGTTGAAGATTTTGGAGGAGGGTTTGCTGGTTGAGATTCTTGCTGGGTGTCCAAAGAAAAAAGCTGGGTCTGTGTGTGAAGGGTGTGGGGAGGCAAGGTTCTTGCCCTGCTTCCAGTGCAATGGGAGCTCAAAGATGGTGCTGGTGGTGAAGGATGACGTGGCGGGGCAGCGGCAGGGGACGACGGTGGTGGTGAAGTGTCCTGAGTGTAATGAGAATGGGTTGGTGCTTTGCCCAATTTGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

329

Amino Acids

35.88

Weight (kDa)

5.63

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 171 - 241 8e-11 Glutaredoxin
GRXCR1-2_C PF23733 272 - 328 1.3e-17 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
Acc36I ACCTGC 1 cut(s) 688
AccB1I GGYRCC 1 cut(s) 688
AccBSI CCGCTC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 519
AccII CGCG 2 cut(s) 65, 633
AclWI GGATC 1 cut(s) 636
AcoI YGGCCR 1 cut(s) 300
AfaI GTAC 1 cut(s) 428
AfiI CCNNNNNNNGG 5 cut(s) 202, 252, 406, 431, 497
AflIII ACRYGT 1 cut(s) 618
AgeI ACCGGT 1 cut(s) 471
AgsI TTSAA 5 cut(s) 28, 364, 418, 652, 745
AjiI CACGTC 2 cut(s) 619, 898
AjnI CCWGG 1 cut(s) 230
AloI GAACNNNNNNTCC 4 cut(s) 620, 652, 821, 853
AluBI AGCT 5 cut(s) 60, 295, 803, 867, 984
AluI AGCT 5 cut(s) 60, 295, 803, 867, 984
Alw21I GWGCWC 1 cut(s) 869
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 1 cut(s) 636
Ama87I CYCGRG 2 cut(s) 176, 250
AoxI GGCC 5 cut(s) 106, 254, 300, 304, 589
ApeKI GCWGC 3 cut(s) 57, 532, 907
ArsI GACNNNNNNTTYG 2 cut(s) 501, 533
AsiGI ACCGGT 1 cut(s) 471
AspLEI GCGC 2 cut(s) 67, 731
AspS9I GGNCC 4 cut(s) 180, 243, 264, 460
AsuC2I CCSGG 4 cut(s) 272, 443, 495, 575
AsuHPI GGTGA 2 cut(s) 898, 943
AsuII TTCGAA 1 cut(s) 477
AvaI CYCGRG 2 cut(s) 176, 250
AvaII GGWCC 4 cut(s) 180, 243, 264, 460
BanI GGYRCC 1 cut(s) 688
BanII GRGCYC 3 cut(s) 197, 210, 869
BbsI GAAGAC 1 cut(s) 430
Bbv12I GWGCWC 1 cut(s) 869
BbvCI CCTCAGC 1 cut(s) 732
BbvI GCAGC 3 cut(s) 69, 519, 919
BccI CCATC 2 cut(s) 619, 868
BceAI ACGGC 2 cut(s) 121, 554
BciT130I CCWGG 1 cut(s) 232
BcnI CCSGG 4 cut(s) 272, 443, 495, 575
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 1 cut(s) 119
BfoI RGCGCY 1 cut(s) 732
BfuAI ACCTGC 1 cut(s) 688
BglI GCCNNNNNGGC 1 cut(s) 575
BlpI GCTNAGC 1 cut(s) 68
Bme1390I CCNGG 5 cut(s) 232, 272, 443, 495, 575
Bme18I GGWCC 4 cut(s) 180, 243, 264, 460
BmeT110I CYCGRG 2 cut(s) 176, 250
BmgBI CACGTC 2 cut(s) 619, 898
BmgT120I GGNCC 4 cut(s) 180, 243, 264, 460
BmiI GGNNCC 4 cut(s) 181, 207, 462, 690
BmrFI CCNGG 5 cut(s) 232, 272, 443, 495, 575
BpiI GAAGAC 1 cut(s) 430
Bpu10I CCTNAGC 1 cut(s) 732
Bpu1102I GCTNAGC 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 477
BpuMI CCSGG 4 cut(s) 272, 443, 495, 575
BsaJI CCNNGG 3 cut(s) 230, 251, 592
BsaWI WCCGGW 1 cut(s) 471
BsaXI ACNNNNNCTCC 8 cut(s) 29, 59, 145, 175, 197, 227, 821, 851
Bsc4I CCNNNNNNNGG 5 cut(s) 202, 252, 406, 431, 497
Bse118I RCCGGY 2 cut(s) 302, 471
Bse1I ACTGG 1 cut(s) 853
Bse3DI GCAATG 1 cut(s) 865
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 3 cut(s) 230, 251, 592
BseGI GGATG 2 cut(s) 685, 898
BseLI CCNNNNNNNGG 5 cut(s) 202, 252, 406, 431, 497
BseMI GCAATG 1 cut(s) 865
BseMII CTCAG 3 cut(s) 82, 723, 933
BseNI ACTGG 1 cut(s) 853
BseRI GAGGAG 7 cut(s) 150, 153, 513, 552, 572, 749, 770
BseXI GCAGC 3 cut(s) 69, 519, 919
BseYI CCCAGC 2 cut(s) 782, 803
Bsh1236I CGCG 2 cut(s) 65, 633
BshFI GGCC 5 cut(s) 108, 256, 302, 306, 591
BshNI GGYRCC 1 cut(s) 688
BshTI ACCGGT 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 869
BsiHKCI CYCGRG 2 cut(s) 176, 250
BsiSI CCGG 6 cut(s) 272, 303, 442, 472, 494, 574
BsiWI CGTACG 1 cut(s) 426
BslFI GGGAC 1 cut(s) 931
BslI CCNNNNNNNGG 5 cut(s) 202, 252, 406, 431, 497
BsmAI GTCTC 1 cut(s) 88
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 931
BsnI GGCC 5 cut(s) 108, 256, 302, 306, 591
BsoBI CYCGRG 2 cut(s) 176, 250
Bsp119I TTCGAA 1 cut(s) 477
Bsp1286I GDGCHC 3 cut(s) 197, 210, 869
Bsp143I GATC 1 cut(s) 628
Bsp1720I GCTNAGC 1 cut(s) 68
BspANI GGCC 5 cut(s) 108, 256, 302, 306, 591
BspCNI CTCAG 3 cut(s) 81, 724, 934
BspFNI CGCG 2 cut(s) 65, 633
BspLI GGNNCC 4 cut(s) 181, 207, 462, 690
BspMI ACCTGC 1 cut(s) 688
BspPI GGATC 1 cut(s) 636
BspT104I TTCGAA 1 cut(s) 477
BspT107I GGYRCC 1 cut(s) 688
BsrBI CCGCTC 1 cut(s) 337
BsrDI GCAATG 1 cut(s) 865
BsrFI RCCGGY 2 cut(s) 302, 471
BsrI ACTGG 1 cut(s) 853
BssAI RCCGGY 2 cut(s) 302, 471
BssECI CCNNGG 3 cut(s) 230, 251, 592
BssMI GATC 1 cut(s) 628
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 3 cut(s) 264, 434, 925
Bst6I CTCTTC 2 cut(s) 29, 639
BstBI TTCGAA 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 135, 304
BstDEI CTNAG 3 cut(s) 68, 732, 942
BstF5I GGATG 2 cut(s) 685, 898
BstFNI CGCG 2 cut(s) 65, 633
BstH2I RGCGCY 1 cut(s) 732
BstHHI GCGC 2 cut(s) 67, 731
BstKTI GATC 1 cut(s) 631
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 1 cut(s) 628
BstMWI GCNNNNNNNGC 7 cut(s) 214, 340, 343, 541, 575, 855, 864
BstNI CCWGG 1 cut(s) 232
BstSCI CCNGG 5 cut(s) 230, 270, 441, 493, 573
BstUI CGCG 2 cut(s) 65, 633
BstV1I GCAGC 3 cut(s) 69, 519, 919
BstV2I GAAGAC 1 cut(s) 430
BstXI CCANNNNNNTGG 1 cut(s) 860
BsuRI GGCC 5 cut(s) 108, 256, 302, 306, 591
BtrI CACGTC 2 cut(s) 619, 898
BtsCI GGATG 2 cut(s) 685, 898
BtsIMutI CAGTG 1 cut(s) 860
BveI ACCTGC 1 cut(s) 688
Cac8I GCNNGC 2 cut(s) 135, 304
CfoI GCGC 2 cut(s) 67, 731
Cfr10I RCCGGY 2 cut(s) 302, 471
Cfr13I GGNCC 4 cut(s) 180, 243, 264, 460
CpoI CGGWCCG 1 cut(s) 264
CseI GACGC 2 cut(s) 320, 538
Csp6I GTAC 1 cut(s) 427
CspAI ACCGGT 1 cut(s) 471
CspI CGGWCCG 1 cut(s) 264
CviAII CATG 1 cut(s) 288
CviQI GTAC 1 cut(s) 427
DdeI CTNAG 3 cut(s) 68, 732, 942
DpnI GATC 1 cut(s) 630
DpnII GATC 1 cut(s) 628
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 29, 639
EarI CTCTTC 2 cut(s) 29, 639
EciI GGCGGA 2 cut(s) 138, 562
Ecl136II GAGCTC 1 cut(s) 867
Eco147I AGGCCT 1 cut(s) 591
Eco24I GRGCYC 3 cut(s) 197, 210, 869
Eco47I GGWCC 4 cut(s) 180, 243, 264, 460
Eco53kI GAGCTC 1 cut(s) 867
Eco88I CYCGRG 2 cut(s) 176, 250
EcoICRI GAGCTC 1 cut(s) 867
EcoO109I RGGNCCY 1 cut(s) 460
EcoRII CCWGG 1 cut(s) 230
EcoT38I GRGCYC 3 cut(s) 197, 210, 869
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 1 cut(s) 291
FaiI YATR 6 cut(s) 241, 289, 299, 487, 720, 722
FaqI GGGAC 1 cut(s) 931
FatI CATG 1 cut(s) 287
FauI CCCGC 4 cut(s) 132, 348, 626, 895
FblI GTMKAC 1 cut(s) 519
FokI GGATG 2 cut(s) 692, 905
FriOI GRGCYC 3 cut(s) 197, 210, 869
FseI GGCCGGCC 1 cut(s) 306
FspBI CTAG 1 cut(s) 119
GlaI GCGC 2 cut(s) 66, 730
GsaI CCCAGC 2 cut(s) 786, 807
HaeII RGCGCY 1 cut(s) 732
HaeIII GGCC 5 cut(s) 108, 256, 302, 306, 591
HapII CCGG 6 cut(s) 272, 303, 442, 472, 494, 574
HgaI GACGC 2 cut(s) 320, 538
HhaI GCGC 2 cut(s) 67, 731
Hin1II CATG 1 cut(s) 291
Hin6I GCGC 2 cut(s) 65, 729
HinP1I GCGC 2 cut(s) 65, 729
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 4 cut(s) 168, 327, 403, 775
HpaII CCGG 6 cut(s) 272, 303, 442, 472, 494, 574
HphI GGTGA 2 cut(s) 898, 943
Hpy166II GTNNAC 2 cut(s) 448, 520
Hpy188I TCNGA 8 cut(s) 192, 205, 226, 247, 268, 326, 596, 641
Hpy188III TCNNGA 2 cut(s) 119, 941
Hpy8I GTNNAC 2 cut(s) 448, 520
Hpy99I CGWCG 6 cut(s) 38, 41, 314, 434, 532, 925
HpyAV CCTTC 9 cut(s) 119, 364, 451, 581, 658, 670, 703, 812, 883
HpyCH4III ACNGT 3 cut(s) 264, 434, 925
HpyCH4IV ACGT 2 cut(s) 618, 897
HpyCH4V TGCA 1 cut(s) 858
HpyF10VI GCNNNNNNNGC 7 cut(s) 214, 340, 343, 541, 575, 855, 864
HpyF3I CTNAG 3 cut(s) 68, 732, 942
HpySE526I ACGT 2 cut(s) 618, 897
Hsp92II CATG 1 cut(s) 291
HspAI GCGC 2 cut(s) 65, 729
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Kzo9I GATC 1 cut(s) 628
LmnI GCTCC 6 cut(s) 65, 142, 205, 292, 342, 864
Lsp1109I GCAGC 3 cut(s) 69, 519, 919
MaeI CTAG 1 cut(s) 119
MaeII ACGT 2 cut(s) 618, 897
MaeIII GTNAC 2 cut(s) 563, 614
MalI GATC 1 cut(s) 630
MbiI CCGCTC 1 cut(s) 337
MboI GATC 1 cut(s) 628
MboII GAAGA 5 cut(s) 6, 16, 430, 656, 757
MhlI GDGCHC 3 cut(s) 197, 210, 869
MluCI AATT 2 cut(s) 411, 975
MmeI TCCRAC 4 cut(s) 183, 270, 291, 420
MroNI GCCGGC 1 cut(s) 302
MseI TTAA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 910
MspI CCGG 6 cut(s) 272, 303, 442, 472, 494, 574
MspR9I CCNGG 5 cut(s) 232, 272, 443, 495, 575
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 2 cut(s) 65, 633
MwoI GCNNNNNNNGC 7 cut(s) 214, 340, 343, 541, 575, 855, 864
NaeI GCCGGC 1 cut(s) 304
NciI CCSGG 4 cut(s) 272, 443, 495, 575
NdeII GATC 1 cut(s) 628
NgoMIV GCCGGC 1 cut(s) 302
NlaIII CATG 1 cut(s) 291
NlaIV GGNNCC 4 cut(s) 181, 207, 462, 690
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 614
NspV TTCGAA 1 cut(s) 477
PceI AGGCCT 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PdiI GCCGGC 1 cut(s) 304
PfeI GAWTC 4 cut(s) 168, 327, 403, 775
Pfl23II CGTACG 1 cut(s) 426
PflFI GACNNNGTC 1 cut(s) 434
PinAI ACCGGT 1 cut(s) 471
PpuMI RGGWCCY 1 cut(s) 460
Psp124BI GAGCTC 1 cut(s) 869
Psp5II RGGWCCY 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 230
PspFI CCCAGC 2 cut(s) 782, 803
PspGI CCWGG 1 cut(s) 230
PspLI CGTACG 1 cut(s) 426
PspN4I GGNNCC 4 cut(s) 181, 207, 462, 690
PspPI GGNCC 4 cut(s) 180, 243, 264, 460
PspPPI RGGWCCY 1 cut(s) 460
PsyI GACNNNGTC 1 cut(s) 434
RigI GGCCGGCC 1 cut(s) 306
RsaI GTAC 1 cut(s) 428
RsaNI GTAC 1 cut(s) 427
Rsr2I CGGWCCG 1 cut(s) 264
RsrII CGGWCCG 1 cut(s) 264
SacI GAGCTC 1 cut(s) 869
SaqAI TTAA 1 cut(s) 282
Sau3AI GATC 1 cut(s) 628
Sau96I GGNCC 4 cut(s) 180, 243, 264, 460
ScrFI CCNGG 5 cut(s) 232, 272, 443, 495, 575
SduI GDGCHC 3 cut(s) 197, 210, 869
SfuI TTCGAA 1 cut(s) 477
SinI GGWCC 4 cut(s) 180, 243, 264, 460
Sse9I AATT 2 cut(s) 411, 975
SseBI AGGCCT 1 cut(s) 591
SspMI CTAG 1 cut(s) 119
SstI GAGCTC 1 cut(s) 869
StuI AGGCCT 1 cut(s) 591
StyD4I CCNGG 5 cut(s) 230, 270, 441, 493, 573
TaaI ACNGT 3 cut(s) 264, 434, 925
TaiI ACGT 2 cut(s) 621, 900
TaqI TCGA 6 cut(s) 166, 309, 477, 555, 609, 623
TasI AATT 2 cut(s) 411, 975
TfiI GAWTC 4 cut(s) 168, 327, 403, 775
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 860
TseFI GTSAC 1 cut(s) 614
TseI GCWGC 3 cut(s) 57, 532, 907
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 1 cut(s) 677
TspGWI ACGGA 1 cut(s) 414
TspRI CASTG 1 cut(s) 860
Tth111I GACNNNGTC 1 cut(s) 434
VpaK11BI GGWCC 4 cut(s) 180, 243, 264, 460
XbaI TCTAGA 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 519
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.