Rroxscaffold_3G00267420

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004963
Physical Location & Seq
Forward (+)
60472619 .. 60473545
927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267420.1

Sequence Viewer

Length: 927 bp
ATGGGCTGTGTTTCTTCCAATCTATTCAACCACGACGACGACTTCTCCCAGCTCGGCAGCTCAGCCTTGAGCCACCACATCGTCTCCCTCACCTCCTCCACCTACGGCCTCCTCACCCTCGACCCCCCACCCCCACCCACCACCCCACCTTCTCGATTCACATTGGGTTCCATCTTCCCCAGCTCCCTGTCCGAGCCCAGATCGCTCTGGTCCGACCCGAAGCCCCTCCGGTCCGACCCGGAAGTCATCAACTCATGGGAGCTCATGGCCGGCCTCGACGCCGACAGCTTCCGGTTCTCGCCGCTCCCGCCGCCGAAACCCTTCTCGATTCGGACCCCTAAAACCGCGGATAAAGAGAACTCCAACCCGAACCGGTCTGTTTTAGTCAAAGGCCCGCTCGACCGGTTCGAGAGTATATGCCCGCCGGGGGGAGAGAACAAACTGGTGCTGTATACGACGTCGTTGCGGGGAGTGCGGAAGACTTTCGAGGCGTGCAATGCGGTCCGGGCGGCGGTGGAGAATCTCGGCGTCATGGTCTGCGAGCGTGACGTGTCGATGGATCGCGGATTCCGAGAAGAGCTGAATGACTTGATGGAGGGGAAAGGCAGAGACGCGGCGGTGCCGCCGAGGTTGTTTGTGAAGGGAAGATACGTGGGTGGATCCGAGGAGGTGTTGAAGATTGTGGAGGAGGGGTTGTTGGGGGAGATTCTAGAAGGTCTGCCCAAGAAGCGGGCCGGCTCGGTTTGCGAAGGGTGCGGAGAAGCGAAATTCTTGCCGTGTTTTCAGTGCAATGGGAGTTCGAAGATGGTGCTGGTGGTGAAAGAGGAGGTGGTGGTGGGGCAGAGGCAGGGAGGTGGCGGCACGGTGGTGGTGAGGTGTCCCGAGTGTAATGAGAATGGGTTGGTGCTCTGCCCAATTTGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

308

Amino Acids

33.27

Weight (kDa)

5.61

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 149 - 218 1.3e-08 Glutaredoxin
GRXCR1-2_C PF23733 249 - 308 1.5e-16 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
AatII GACGTC 1 cut(s) 461
AccB1I GGYRCC 1 cut(s) 619
AccBSI CCGCTC 2 cut(s) 304, 397
AccI GTMKAC 1 cut(s) 452
AccII CGCG 3 cut(s) 347, 564, 614
AclWI GGATC 3 cut(s) 567, 654, 667
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 1 cut(s) 767
AcyI GRCGYC 3 cut(s) 279, 458, 528
AfiI CCNNNNNNNGG 4 cut(s) 427, 428, 511, 729
AflIII ACRYGT 1 cut(s) 549
AgeI ACCGGT 2 cut(s) 372, 402
AgsI TTSAA 2 cut(s) 28, 676
AjiI CACGTC 1 cut(s) 550
AleI CACNNNNGTG 1 cut(s) 866
AluBI AGCT 7 cut(s) 52, 60, 183, 262, 288, 580, 924
AluI AGCT 7 cut(s) 52, 60, 183, 262, 288, 580, 924
Alw21I GWGCWC 2 cut(s) 264, 909
Alw26I GTCTC 2 cut(s) 88, 603
AlwI GGATC 3 cut(s) 567, 654, 667
Ama87I CYCGRG 1 cut(s) 881
AoxI GGCC 5 cut(s) 106, 267, 271, 391, 732
ApeKI GCWGC 1 cut(s) 57
ApoI RAATTY 1 cut(s) 767
AsiGI ACCGGT 2 cut(s) 372, 402
Asp700I GAANNNNTTC 2 cut(s) 320, 482
AspS9I GGNCC 6 cut(s) 210, 231, 333, 392, 502, 732
AsuC2I CCSGG 3 cut(s) 239, 426, 506
AsuHPI GGTGA 4 cut(s) 82, 106, 829, 883
AsuII TTCGAA 1 cut(s) 800
AvaI CYCGRG 1 cut(s) 881
AvaII GGWCC 4 cut(s) 210, 231, 333, 502
BaeI ACNNNNGTAYC 2 cut(s) 640, 673
BamHI GGATCC 1 cut(s) 659
BanI GGYRCC 1 cut(s) 619
BanII GRGCYC 2 cut(s) 198, 264
BarI GAAGNNNNNNTAC 2 cut(s) 632, 664
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 2 cut(s) 264, 909
BbvI GCAGC 1 cut(s) 69
BccI CCATC 4 cut(s) 179, 550, 586, 799
BceAI ACGGC 2 cut(s) 121, 760
BcgI CGANNNNNNTGC 6 cut(s) 445, 479, 754, 788, 790, 824
BcnI CCSGG 3 cut(s) 239, 426, 506
BcoDI GTCTC 2 cut(s) 88, 603
BfaI CTAG 1 cut(s) 710
BisI GCNGC 7 cut(s) 58, 302, 311, 510, 615, 623, 859
BlpI GCTNAGC 1 cut(s) 61
BlsI GCNGC 7 cut(s) 59, 303, 312, 511, 616, 624, 860
Bme1390I CCNGG 3 cut(s) 239, 426, 506
Bme18I GGWCC 4 cut(s) 210, 231, 333, 502
BmeT110I CYCGRG 1 cut(s) 881
BmgBI CACGTC 1 cut(s) 550
BmgT120I GGNCC 6 cut(s) 210, 231, 333, 392, 502, 732
BmiI GGNNCC 4 cut(s) 169, 335, 621, 661
BmrFI CCNGG 3 cut(s) 239, 426, 506
BpiI GAAGAC 1 cut(s) 485
Bpu1102I GCTNAGC 1 cut(s) 61
Bpu14I TTCGAA 1 cut(s) 800
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 3 cut(s) 239, 426, 506
BsaAI YACGTR 1 cut(s) 652
BsaHI GRCGYC 3 cut(s) 279, 458, 528
BsaJI CCNNGG 4 cut(s) 345, 425, 626, 663
BsaWI WCCGGW 4 cut(s) 228, 291, 372, 402
BsaXI ACNNNNNCTCC 8 cut(s) 29, 59, 68, 98, 677, 680, 707, 710
Bsc4I CCNNNNNNNGG 4 cut(s) 427, 428, 511, 729
Bse118I RCCGGY 4 cut(s) 269, 372, 402, 734
Bse1I ACTGG 1 cut(s) 447
Bse3DI GCAATG 2 cut(s) 502, 796
BseDI CCNNGG 4 cut(s) 345, 425, 626, 663
BseLI CCNNNNNNNGG 4 cut(s) 427, 428, 511, 729
BseMI GCAATG 2 cut(s) 502, 796
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 447
BseRI GAGGAG 5 cut(s) 85, 101, 680, 701, 839
BseXI GCAGC 1 cut(s) 69
BseYI CCCAGC 2 cut(s) 48, 179
Bsh1236I CGCG 3 cut(s) 347, 564, 614
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 5 cut(s) 108, 269, 273, 393, 734
BshNI GGYRCC 1 cut(s) 619
BshTI ACCGGT 2 cut(s) 372, 402
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 2 cut(s) 264, 909
BsiHKCI CYCGRG 1 cut(s) 881
BsiSI CCGG 9 cut(s) 229, 239, 270, 292, 373, 403, 425, 505, 735
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 4 cut(s) 427, 428, 511, 729
BsmAI GTCTC 2 cut(s) 88, 603
BsmBI CGTCTC 2 cut(s) 88, 603
BsmFI GGGAC 1 cut(s) 864
BsnI GGCC 5 cut(s) 108, 269, 273, 393, 734
BsoBI CYCGRG 1 cut(s) 881
Bsp119I TTCGAA 1 cut(s) 800
Bsp1286I GDGCHC 3 cut(s) 198, 264, 909
Bsp143I GATC 3 cut(s) 200, 559, 659
Bsp1720I GCTNAGC 1 cut(s) 61
BspANI GGCC 5 cut(s) 108, 269, 273, 393, 734
BspCNI CTCAG 1 cut(s) 74
BspFNI CGCG 3 cut(s) 347, 564, 614
BspLI GGNNCC 4 cut(s) 169, 335, 621, 661
BspPI GGATC 3 cut(s) 567, 654, 667
BspQI GCTCTTC 1 cut(s) 570
BspT104I TTCGAA 1 cut(s) 800
BspT107I GGYRCC 1 cut(s) 619
BsrBI CCGCTC 2 cut(s) 304, 397
BsrDI GCAATG 2 cut(s) 502, 796
BsrFI RCCGGY 4 cut(s) 269, 372, 402, 734
BsrI ACTGG 1 cut(s) 447
BssAI RCCGGY 4 cut(s) 269, 372, 402, 734
BssECI CCNNGG 4 cut(s) 345, 425, 626, 663
BssMI GATC 3 cut(s) 200, 559, 659
BssNAI GTATAC 1 cut(s) 453
BssNI GRCGYC 3 cut(s) 279, 458, 528
Bst1107I GTATAC 1 cut(s) 453
Bst4CI ACNGT 1 cut(s) 865
Bst6I CTCTTC 1 cut(s) 570
BstACI GRCGYC 3 cut(s) 279, 458, 528
BstBAI YACGTR 1 cut(s) 652
BstBI TTCGAA 1 cut(s) 800
BstC8I GCNNGC 7 cut(s) 271, 395, 422, 493, 542, 732, 736
BstDEI CTNAG 1 cut(s) 61
BstDSI CCRYGG 1 cut(s) 345
BstFNI CGCG 3 cut(s) 347, 564, 614
BstKTI GATC 3 cut(s) 203, 562, 662
BstMAI GTCTC 2 cut(s) 88, 603
BstMBI GATC 3 cut(s) 200, 559, 659
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 9 cut(s) 202, 307, 310, 472, 497, 506, 727, 744, 753
BstSCI CCNGG 3 cut(s) 237, 424, 504
BstUI CGCG 3 cut(s) 347, 564, 614
BstV1I GCAGC 1 cut(s) 69
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 1 cut(s) 659
BstYI RGATCY 1 cut(s) 659
BstZ17I GTATAC 1 cut(s) 453
BsuRI GGCC 5 cut(s) 108, 269, 273, 393, 734
BtgI CCRYGG 1 cut(s) 345
BtrI CACGTC 1 cut(s) 550
BtsIMutI CAGTG 1 cut(s) 791
Cac8I GCNNGC 7 cut(s) 271, 395, 422, 493, 542, 732, 736
Cfr10I RCCGGY 4 cut(s) 269, 372, 402, 734
Cfr13I GGNCC 6 cut(s) 210, 231, 333, 392, 502, 732
Cfr42I CCGCGG 1 cut(s) 348
CpoI CGGWCCG 2 cut(s) 231, 502
CseI GACGC 3 cut(s) 287, 517, 620
CspAI ACCGGT 2 cut(s) 372, 402
CspI CGGWCCG 2 cut(s) 231, 502
CviAII CATG 3 cut(s) 255, 265, 532
DdeI CTNAG 1 cut(s) 61
DpnI GATC 3 cut(s) 202, 561, 661
DpnII GATC 3 cut(s) 200, 559, 659
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Ecl136II GAGCTC 1 cut(s) 262
Eco24I GRGCYC 2 cut(s) 198, 264
Eco47I GGWCC 4 cut(s) 210, 231, 333, 502
Eco53kI GAGCTC 1 cut(s) 262
Eco88I CYCGRG 1 cut(s) 881
EcoICRI GAGCTC 1 cut(s) 262
EcoT38I GRGCYC 2 cut(s) 198, 264
Esp3I CGTCTC 2 cut(s) 88, 603
FaeI CATG 3 cut(s) 258, 268, 535
FaiI YATR 6 cut(s) 256, 266, 416, 418, 453, 533
FaqI GGGAC 1 cut(s) 864
FatI CATG 3 cut(s) 254, 264, 531
FauI CCCGC 5 cut(s) 315, 402, 429, 459, 723
FblI GTMKAC 1 cut(s) 452
Fnu4HI GCNGC 7 cut(s) 58, 302, 311, 510, 615, 623, 859
FriOI GRGCYC 2 cut(s) 198, 264
FseI GGCCGGCC 1 cut(s) 273
Fsp4HI GCNGC 7 cut(s) 58, 302, 311, 510, 615, 623, 859
FspBI CTAG 1 cut(s) 710
GluI GCNGC 7 cut(s) 58, 302, 311, 510, 615, 623, 859
GsaI CCCAGC 2 cut(s) 52, 183
HaeIII GGCC 5 cut(s) 108, 269, 273, 393, 734
HapII CCGG 9 cut(s) 229, 239, 270, 292, 373, 403, 425, 505, 735
HgaI GACGC 3 cut(s) 287, 517, 620
Hin1I GRCGYC 3 cut(s) 279, 458, 528
Hin1II CATG 3 cut(s) 258, 268, 535
HinfI GANTC 5 cut(s) 156, 328, 520, 567, 706
HpaII CCGG 9 cut(s) 229, 239, 270, 292, 373, 403, 425, 505, 735
HphI GGTGA 4 cut(s) 82, 106, 829, 883
Hpy166II GTNNAC 1 cut(s) 453
Hpy188I TCNGA 6 cut(s) 193, 214, 235, 333, 572, 664
Hpy188III TCNNGA 5 cut(s) 153, 325, 409, 710, 881
Hpy8I GTNNAC 1 cut(s) 453
Hpy99I CGWCG 5 cut(s) 38, 41, 281, 460, 463
HpyAV CCTTC 5 cut(s) 159, 331, 634, 707, 743
HpyCH4III ACNGT 1 cut(s) 865
HpyCH4IV ACGT 3 cut(s) 458, 549, 651
HpyCH4V TGCA 2 cut(s) 495, 789
HpyF10VI GCNNNNNNNGC 9 cut(s) 202, 307, 310, 472, 497, 506, 727, 744, 753
HpyF3I CTNAG 1 cut(s) 61
HpySE526I ACGT 3 cut(s) 458, 549, 651
Hsp92I GRCGYC 3 cut(s) 279, 458, 528
Hsp92II CATG 3 cut(s) 258, 268, 535
KroI GCCGGC 2 cut(s) 269, 734
KroNI GCCGGC 2 cut(s) 271, 736
KspI CCGCGG 1 cut(s) 348
Kzo9I GATC 3 cut(s) 200, 559, 659
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 3 cut(s) 188, 259, 309
Lsp1109I GCAGC 1 cut(s) 69
MaeI CTAG 1 cut(s) 710
MaeII ACGT 3 cut(s) 458, 549, 651
MaeIII GTNAC 1 cut(s) 545
MalI GATC 3 cut(s) 202, 561, 661
MbiI CCGCTC 2 cut(s) 304, 397
MboI GATC 3 cut(s) 200, 559, 659
MboII GAAGA 7 cut(s) 6, 166, 490, 587, 657, 688, 814
MflI RGATCY 1 cut(s) 659
MhlI GDGCHC 3 cut(s) 198, 264, 909
MluCI AATT 2 cut(s) 767, 915
MmeI TCCRAC 3 cut(s) 237, 258, 387
MroNI GCCGGC 2 cut(s) 269, 734
MroXI GAANNNNTTC 2 cut(s) 320, 482
MslI CAYNNNNRTG 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 9 cut(s) 229, 239, 270, 292, 373, 403, 425, 505, 735
MspR9I CCNGG 3 cut(s) 239, 426, 506
MvnI CGCG 3 cut(s) 347, 564, 614
MwoI GCNNNNNNNGC 9 cut(s) 202, 307, 310, 472, 497, 506, 727, 744, 753
NaeI GCCGGC 2 cut(s) 271, 736
NciI CCSGG 3 cut(s) 239, 426, 506
NdeII GATC 3 cut(s) 200, 559, 659
NgoMIV GCCGGC 2 cut(s) 269, 734
NlaIII CATG 3 cut(s) 258, 268, 535
NlaIV GGNNCC 4 cut(s) 169, 335, 621, 661
NmeAIII GCCGAG 3 cut(s) 33, 504, 651
NmuCI GTSAC 1 cut(s) 545
NspV TTCGAA 1 cut(s) 800
OliI CACNNNNGTG 1 cut(s) 866
PciSI GCTCTTC 1 cut(s) 570
PcsI WCGNNNNNNNCGW 2 cut(s) 405, 568
PdiI GCCGGC 2 cut(s) 271, 736
PdmI GAANNNNTTC 2 cut(s) 320, 482
PfeI GAWTC 5 cut(s) 156, 328, 520, 567, 706
PinAI ACCGGT 2 cut(s) 372, 402
PkrI GCNGC 7 cut(s) 59, 303, 312, 511, 616, 624, 860
Ppu21I YACGTR 1 cut(s) 652
Psp124BI GAGCTC 1 cut(s) 264
PspFI CCCAGC 2 cut(s) 48, 179
PspN4I GGNNCC 4 cut(s) 169, 335, 621, 661
PspPI GGNCC 6 cut(s) 210, 231, 333, 392, 502, 732
PsuI RGATCY 1 cut(s) 659
RigI GGCCGGCC 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 866
Rsr2I CGGWCCG 2 cut(s) 231, 502
RsrII CGGWCCG 2 cut(s) 231, 502
SacI GAGCTC 1 cut(s) 264
SacII CCGCGG 1 cut(s) 348
SapI GCTCTTC 1 cut(s) 570
SatI GCNGC 7 cut(s) 58, 302, 311, 510, 615, 623, 859
Sau3AI GATC 3 cut(s) 200, 559, 659
Sau96I GGNCC 6 cut(s) 210, 231, 333, 392, 502, 732
ScrFI CCNGG 3 cut(s) 239, 426, 506
SduI GDGCHC 3 cut(s) 198, 264, 909
Sfr303I CCGCGG 1 cut(s) 348
SfuI TTCGAA 1 cut(s) 800
SgrBI CCGCGG 1 cut(s) 348
SinI GGWCC 4 cut(s) 210, 231, 333, 502
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 2 cut(s) 767, 915
SspMI CTAG 1 cut(s) 710
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 237, 424, 504
TaaI ACNGT 1 cut(s) 865
TaiI ACGT 3 cut(s) 461, 552, 654
TaqI TCGA 9 cut(s) 120, 154, 276, 326, 399, 408, 486, 554, 800
TasI AATT 2 cut(s) 767, 915
TauI GCSGC 6 cut(s) 304, 313, 512, 617, 625, 861
TfiI GAWTC 5 cut(s) 156, 328, 520, 567, 706
TscAI CASTG 1 cut(s) 791
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 1 cut(s) 57
Tsp45I GTSAC 1 cut(s) 545
TspRI CASTG 1 cut(s) 791
VpaK11BI GGWCC 4 cut(s) 210, 231, 333, 502
XapI RAATTY 1 cut(s) 767
XbaI TCTAGA 1 cut(s) 709
XmiI GTMKAC 1 cut(s) 452
XmnI GAANNNNTTC 2 cut(s) 320, 482
XspI CTAG 1 cut(s) 710
ZraI GACGTC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.